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1.
Int J Pharm Compd ; 26(5): 400-408, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36053767

RESUMO

Over the years, numerous approaches have been used to increase drug penetration of the skin. Transdermal drug delivery has potential advantages over other conventional routes of drug delivery, as it can provide a non- invasive and painless alternative to parenteral routes. Also, the pharmacokinetic profiles of drugs can be more uniform with less variability, resulting in improved patient compliance. Classical topical formulations are different from those intended for transdermal drug delivery, as they are generally applied to broken, diseased, or damaged skin, whereas, transdermal drugs are applied to healthy skin. On the negative side, the use of transdermal drug delivery is limited by the restrictions inherent in the lipophilic stratum corneum barrier, which allows a limited number of drug molecules with certain physicochemical properties to be delivered. This article, which represents part 1 of a series of articles on compounding with microneedles, provides an introduction and overview of this topic.


Assuntos
Sistemas de Liberação de Medicamentos , Agulhas , Administração Cutânea , Humanos , Preparações Farmacêuticas , Pele/metabolismo , Absorção Cutânea
2.
Eur J Dermatol ; 32(3): 305-311, 2022 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-36065535

RESUMO

Short- and long-term exposure to atmospheric pollution has significant health effects. The skin is the organ directly in contact with pollutants and is responsible for protection of the organism. Particulate matter (PM) such as polycyclic aromatic hydrocarbons (PAHs) are the basis of certain pulmonary as well as dermatological complications. Pollution exacerbates certain illnesses such as atopic dermatitis and cancer, and it may also participate in delaying wound healing and in the occurrence of chronic ailments such as diabetes. The aryl hydrocarbon receptor (AhR) transcription factor, at the core of these responses to pollutants, is expressed by all cells of the skin. The AhR is subject to tight regulation that depends on its ligand. Pollutants act in a deleterious manner via the AhR, influencing the behaviour of keratinocytes as well as fibroblasts. Natural ligands, on the other hand, allow the noxious effects of pollution to be countered. This non-systematic review of the literature shows that modulation of AhR appears to be an excellent therapeutic approach to improve or stop the cutaneous problems linked to pollution.


Assuntos
Dermatite Atópica , Poluentes Ambientais , Receptores de Hidrocarboneto Arílico , Fatores de Transcrição Hélice-Alça-Hélice Básicos , Dermatite Atópica/metabolismo , Poluentes Ambientais/toxicidade , Humanos , Queratinócitos/efeitos dos fármacos , Queratinócitos/metabolismo , Receptores de Hidrocarboneto Arílico/fisiologia , Pele/efeitos dos fármacos , Pele/metabolismo
3.
Nihon Yakurigaku Zasshi ; 157(5): 335-339, 2022.
Artigo em Japonês | MEDLINE | ID: mdl-36047148

RESUMO

The skin is not only the site of drug application, but also the site of exposure to various chemical substances. Prediction of skin permeability, body absorption, and local skin concentration of chemicals are very important to assure efficacy and safety. Most in silico models for skin permeation of chemical are based on the prediction of its permeability coefficient (P, unit cm/s), and a certain level of prediction accuracy has been obtained. On the other hand, the amount absorbed in the body of a chemical is calculated from the sum of the amount of skin permeation and the amount under the stratum corneum (viable epidermis and dermis). The amount of skin permeation can be calculated from the P value as mentioned above, however, the amount under the stratum corneum, which is related to the local skin safety of the chemical, cannot be predicted. This article describes the principles of permeation of chemicals across the skin, the relationship between skin permeability and concentration in the skin, and the method for calculating the chemical concentration under the stratum corneum using skin permeation parameters.


Assuntos
Absorção Cutânea , Pele , Administração Cutânea , Difusão , Epiderme/metabolismo , Pele/metabolismo
4.
Nihon Yakurigaku Zasshi ; 157(5): 345-350, 2022.
Artigo em Japonês | MEDLINE | ID: mdl-36047150

RESUMO

Amino acid Derivative Reactivity Assay (ADRA) is an alternative method developed based on the principle of covalent bonding between sensitizer and proteins in the early stage of the mechanism of skin sensitization. The Direct Peptide Reactivity Assay (DPRA) with same principle previously listed in the OECD test guidelines (TG) have some problems such as precipitation of the test chemical in the reaction solution and co-elution of the peptide with the test chemical. While, instead of DPRA, the ADRA was developed using two chemically synthesized nucleophilic reagents-namely, NAC and NAL in which naphthalene rings with a high molar absorbance coefficient (MAC) in the ultraviolet range have been introduced to N-termini of the cysteine and lysine that can react with the test chemical. Therefore, in March 2016, we set up a validation team with the aim for adoption in the OECD TG, ADRA's validation tests were conducted. After reporting the results of validation study, holding a third-party evaluation meeting and two commenting rounds, ADRA was able to be adopted in the OECD TG in June 2019. In addition, since the introduction of naphthalene with a high MAC has made it possible to reduce the concentration, enabling the following items. 1) Decrease in the frequency of precipitation of the test chemicals in the reaction solution. 2) Decrease in the frequency of co-eluting of the nucleating reagent and the chemical. 3) Evaluation of chemicals with unknown molecular weight using the gravimetric approach. 4) High-sensitivity detection of nucleophilic reagents by the fluorescence method. 5) Evaluation of the mixture by a combination of the gravimetric approach and fluorescence detection.


Assuntos
Alternativas aos Testes com Animais , Organização para a Cooperação e Desenvolvimento Econômico , Alternativas aos Testes com Animais/métodos , Animais , Naftalenos , Peptídeos/química , Pele/metabolismo
5.
Int J Mol Sci ; 23(17)2022 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-36076953

RESUMO

Disruption of the skin microbial balance can exacerbate certain skin diseases and affect prognosis and treatment. Changes in the distribution and prevalence of certain microbial species on the skin, such as Staphylococcus aureus (SA), can impact the development of severe atopic dermatitis (AD) or psoriasis (Pso). A dysfunctional skin barrier develops in AD and Pso due to SA colonization, resulting in keratinization and chronic or progressive chronic inflammation. Disruption of the skin barrier following SA colonization can elevate the production of T helper 2 (Th2)-derived cytokines, which can cause an imbalance in Th1, Th2, and Th17 cells. This study examined the ability of potential therapeutic skin microbiomes, such as Cutibacterium avidum R-CH3 and Staphylococcus hominis R9, to inhibit SA biofilm formation and restore skin barrier function-related genes through the activation of the aryl hydrocarbon receptor (AhR) and the nuclear factor erythroid-2-related factor 2 (Nrf2) downstream target. We observed that IL-4/IL-13-induced downregulation of FLG, LOR, and IVL induced by SA colonization could be reversed by dual AhR/Nrf2 activation. Further, OVOL1 expression may be modulated by functional microbiomes via dual AhR/Nrf2 activation. Our results suggest that our potential therapeutic skin microbiomes can prevent SA-derived Th2-biased skin barrier disruption via IL-13 and IL-4-dependent FLG deregulation, STAT3 activation, and AhR-mediated STAT6 expression.


Assuntos
Microbiota , Psoríase , Humanos , Imunidade , Interleucina-13/metabolismo , Interleucina-4/metabolismo , Proteínas de Filamentos Intermediários/genética , Queratinócitos/metabolismo , Fator 2 Relacionado a NF-E2/genética , Fator 2 Relacionado a NF-E2/metabolismo , Psoríase/metabolismo , Receptores de Hidrocarboneto Arílico/metabolismo , Transdução de Sinais , Pele/metabolismo , Staphylococcus aureus/metabolismo
6.
Int J Mol Sci ; 23(17)2022 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-36077018

RESUMO

Skin soft tissue expansion is one of the most basic and commonly used techniques in plastic surgery to obtain excess skin for a variety of medical uses. However, skin soft tissue expansion is faced with many problems, such as long treatment process, poor skin quality, high retraction rate, and complications. Therefore, a deeper understanding of the mechanisms of skin soft tissue expansion is needed. The key to skin soft tissue expansion lies in the mechanical stretch applied to the skin by an inflatable expander. Mechanical stimulation activates multiple signaling pathways through cellular adhesion molecules and regulates gene expression profiles in cells. Meanwhile, various types of cells contribute to skin expansion, including keratinocytes, dermal fibroblasts, and mesenchymal stem cells, which are also regulated by mechanical stretch. This article reviews the molecular and cellular mechanisms of skin regeneration induced by mechanical stretch during skin soft tissue expansion.


Assuntos
Células-Tronco Mesenquimais , Expansão de Tecido , Queratinócitos , Células-Tronco Mesenquimais/metabolismo , Transdução de Sinais/fisiologia , Pele/metabolismo
7.
Int J Mol Sci ; 23(17)2022 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-36077103

RESUMO

The peroxisome proliferator-activated receptor PPAR-γ is one of three PPAR nuclear receptors that act as ligand-activated transcription factors. In immune cells, the skin, and other organs, PPAR-γ regulates lipid, glucose, and amino acid metabolism. The receptor translates nutritional, pharmacological, and metabolic stimuli into the changes in gene expression. The activation of PPAR-γ promotes cell differentiation, reduces the proliferation rate, and modulates the immune response. In the skin, PPARs also contribute to the functioning of the skin barrier. Since we know that the route from identification to the registration of drugs is long and expensive, PPAR-γ agonists already approved for other diseases may also represent a high interest for psoriasis. In this review, we discuss the role of PPAR-γ in the activation, differentiation, and proliferation of skin and immune cells affected by psoriasis and in contributing to the pathogenesis of the disease. We also evaluate whether the agonists of PPAR-γ may become one of the therapeutic options to suppress the inflammatory response in lesional psoriatic skin and decrease the influence of comorbidities associated with psoriasis.


Assuntos
Psoríase , Dermatopatias , Humanos , PPAR gama/metabolismo , Psoríase/metabolismo , Pele/metabolismo , Dermatopatias/metabolismo , Fatores de Transcrição/metabolismo
8.
Int J Mol Sci ; 23(17)2022 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-36077254

RESUMO

Boswellic acids, triterpenoids derived from the genus Boswellia (Burseraceae), are known for their anti-inflammatory and anti-tumor efficacy. Atopic dermatitis is a chronic, non-infectious inflammatory skin disease. However, the effects of α-boswellic acid on atopic dermatitis have not been studied. Therefore, in this study we examined the expression level of pro-inflammatory cytokines, histopathological analysis, and physiological data from BALB/c mice with atopic-like dermatitis induced by 2,4-dinitrochlorobenzene and TNF-α/IFN-γ-stimulated HaCaT cells to better understand the agent's anti-atopic dermatitis efficacy. First, we found that α-boswellic reduced the epidermal thickening, mast cell numbers, and dermal infiltration of 2,4-dinitrochlorobenzene-induced atopic-like dermatitis in BALB/c mice. Furthermore, we also found that α-boswellic acid can restore transepidermal water loss and skin reddening in mice. In human keratinocytes inflamed by TNF-α/IFN-γ, α-boswellic acid inhibited MAP kinase activation and showed a reduction in NF-κB nuclear translocation. Finally, α-boswellic acid can reduce the expression level of cytokines (IL-1ß, IL-6, and IL-8) following the stimulation of TNF-α/IFN-γ in HaCaT cells. Taken together, our study suggests that α-boswellic acids are a potential component for the development of anti-atopic dermatitis drugs.


Assuntos
Dermatite Atópica , Triterpenos , Animais , Citocinas/metabolismo , Dermatite Atópica/induzido quimicamente , Dermatite Atópica/tratamento farmacológico , Dermatite Atópica/metabolismo , Dinitroclorobenzeno/toxicidade , Células HaCaT , Humanos , Interferon gama/genética , Interferon gama/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , NF-kappa B/metabolismo , Extratos Vegetais/farmacologia , Pele/metabolismo , Triterpenos/metabolismo , Fator de Necrose Tumoral alfa/metabolismo
9.
Biochem Biophys Res Commun ; 628: 57-63, 2022 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-36081279

RESUMO

Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by type 2 immune responses. Interleukin-25 (IL-25) is produced predominantly by epithelial cells. It can activate Th2 cells to produce type 2 cytokines such as IL-4, IL-5 and IL-13, contributing to host defense against nematodes. However, excessive/inappropriate production of IL-25 is considered to be involved in development of type 2 cytokine-associated allergic disorders such as asthma. On the other hand, the contribution of IL-25 to the pathogenesis of AD remains poorly understood. In the present study, we found that expression of Il25 mRNA was significantly increased in the skin of mice during oxazolone-induced chronic contact hypersensitivity (CHS), which is a mouse model of human AD. In addition, development of oxazolone-induced chronic CHS was significantly reduced in IL-25-deficient (Il25-/-) mice compared with wild-type mice on the C57BL/6, but not BALB/c, background, although IL-25 was not essential for IL-4 production by hapten-specific T cells. Therefore, IL-25 is crucial for development of chronic CHS, although that is partly dependent on the genetic background of the mice.


Assuntos
Dermatite Atópica , Dermatite de Contato , Animais , Citocinas/metabolismo , Dermatite Atópica/induzido quimicamente , Dermatite Atópica/genética , Dermatite de Contato/genética , Haptenos , Humanos , Interleucina-13 , Interleucina-17/genética , Interleucina-4/genética , Interleucina-5 , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Oxazolona , RNA Mensageiro , Pele/metabolismo
10.
Pak J Biol Sci ; 25(9): 822-826, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36098084

RESUMO

<b>Background and Objective:</b> <i> Chalcorana chalconota </i>is a complex frog species in West Sumatra which has been revised and designated as <i>Chalcorana parvaccola</i> and <i>Chalcorana rufipes</i> based on several studies such as morphology and genetics. Other studies such as protein band patterns can be a marker to differentiate species. This research was done to determine and prove the variations of protein band patterns found in skin secretions of the <i>C. chalconota </i>species complex. <b>Materials and Methods:</b> Frog samples were collected in the Pasia Laweh area, Pesisir Selatan, West Sumatra. The standard length measurement of the frog was carried out to determine the voltage that will be applied to the frog using an electric shock device (TAS/transcutaneous amphibian stimulator) in the process of removing the frog's skin secretions. Frog skin secretions were taken and used to see the pattern of protein bands using the SDS-PAGE method. <b>Results:</b> The protein band patterns of skin secretions of <i>C. chalconota</i> species complex were different between <i>C. parvaccola</i> and <i>C. rufipes</i>. In the skin secretions of <i>C. parvaccola</i>, there were eight protein bands with a molecular weight ranging between 12-103 kDa while for <i>C. rufipes</i> there were seven protein bands with a molecular weight ranging between 12-102 kDa. There were six protein bands shared by these two species. Two bands were only found in the skin secretions of <i>C. parvaccola</i> and one band was only found in <i>C. rufipes</i>. <b>Conclusion:</b> Pattern and molecular weight of protein in <i>C. parvaccola</i> and <i>C. rufipes</i> skin secretions can be used as protein markers to distinguish the two species.


Assuntos
Pele , Indonésia , Peso Molecular , Pele/metabolismo
11.
Molecules ; 27(17)2022 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-36080249

RESUMO

One of the critical elements in evaluating the quality of cashmere is its fineness, but we still know little about how it is regulated at the metabolic level. In this paper, we use UHPLC-MS/MS detection and analysis technology to compare the difference in metabolites between coarse cashmere (CT_LCG) and fine cashmere (FT_LCG) skin of Liaoning cashmere goats. According to the data, under positive mode four metabolites were significantly up-regulated and seven were significantly down-regulated. In negative mode, seven metabolites were significantly up-regulated and fourteen metabolites were significantly down-regulated. The two groups' most significant metabolites, Gly-Phe and taurochenodeoxycholate, may be crucial in controlling cashmere's growth, development, and fineness. In addition, we enriched six KEGG pathways, of which cholesterol metabolism, primary bile acid biosynthesis, and bile secretion were enriched in positive and negative modes. These findings offer a new research idea for further study into the critical elements influencing cashmere's fineness.


Assuntos
Cabras , Espectrometria de Massas em Tandem , Animais , Pele/metabolismo
12.
FASEB J ; 36(10): e22533, 2022 10.
Artigo em Inglês | MEDLINE | ID: mdl-36065711

RESUMO

During animal fasting, the nutrient supply and metabolism switch from carbohydrates to a new reliance on the catabolism of energy-dense lipid stores. Assembled under tight regulation, ßγ-CAT (a complex of non-lens ßγ-crystallin and trefoil factor) is a pore-forming protein and trefoil factor complex identified in toad Bombina maxima. Here, we determined that this protein complex is a constitutive component in toad blood, that actively responds to the animal fasting. The protein complex was able to promote cellular albumin and albumin-bound fatty acid (FA) uptake in a variety of epithelial and endothelial cells, and the effects were attenuated by a macropinocytosis inhibitor. Endothelial cell-derived exosomes containing largely enriched albumin and FAs, called nutrisomes, were released in the presence of ßγ-CAT. These specific nutrient vesicles were readily taken up by starved myoblast cells to support their survival. The results uncovered that pore-forming protein ßγ-CAT is a fasting responsive element able to drive cell vesicular import and export of macromolecular nutrients.


Assuntos
Células Endoteliais , Fatores Trefoil , Albuminas/metabolismo , Animais , Células Endoteliais/metabolismo , Jejum , Nutrientes , Peptídeos/metabolismo , Pele/metabolismo , Fatores Trefoil/metabolismo
13.
Immunity ; 55(9): 1586-1588, 2022 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-36103856

RESUMO

The full range of receptors through which antimicrobial peptides exert their immunologic effects remains incompletely explored. Dong and colleagues identify Mgrpra2 as a G-coupled protein receptor on neutrophils, for which keratinocyte-derived Beta-defensins serve as key ligands. Binding of Mgrpra2 leads to release of neutrophil granules and Il-1ß, which helps shape skin microbiome composition and augments cutaneous defense against bacterial infection.


Assuntos
beta-Defensinas , Proteínas de Transporte , Queratinócitos/metabolismo , Neutrófilos/metabolismo , Pele/metabolismo , beta-Defensinas/química , beta-Defensinas/metabolismo
14.
Sci Rep ; 12(1): 14244, 2022 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-35987944

RESUMO

Herein, Imiquimod (IMQ) was incorporated in nanotransethosomes (nTES) to develop the IMQ-nTES nano-drug delivery system. IMQ-nTES was optimized using 23 factorial design. The optimized formulation was expressed with a particle size of 192.4 ± 1.60 nm, Poly-dispersibility of 0.115 ± 0.008, and IMQ percent entrapment efficiency of 91.05 ± 3.22%. Smooth and round morphology of IMQ-nTES vesicles was confirmed by TEM micrographs. Moreover, FTIR results have shown drug-excipient compatibility. The IMQ-nTES was laden inside the low molecular weight chitosan gel, which exhibited easy application, spreadability and no irritation to the applied skin. The release pattern has clearly exhibited improved dissolution properties of IMQ with the provision of the sustain release pattern. Higher IMQ content was deposited in deeper epidermis and dermis with IMQ-nTES gel, in contrast to ALDARA. In vivo, comparative toxicity study on BALB/c mice has shown significantly reduced (p < 0.001) psoriatic area severity index (PASI) score and less increment in ear thickness. Epidermal hyperplasia was an obvious finding with ALDARA which was, providentially, minimal in IMQ-nTES gel-treated skin. FTIR analysis of skin tissue has shown an enhancement of lipid and protein content in the ALDARA group, however, in the IMQ-nTES group no such change was observed. With ALDARA application, CD4+ T-cells and constitutive NF-κß expression were significantly elevated, in comparison to the IMQ-nTES gel treated group. Moreover, the adequate expression of IFN-γ and cytotoxic CD8+ T-cells were suggesting the preserved IMQ efficacy with IMQ-nTES gel. Quantification of cutaneous as well as systemic inflammatory markers has also suggested the reduced psoriatic potential of IMQ-nTES gel. In essence, IMQ-nTES gel can be a suitable alternative to ALDARA owing to its better safety profile.


Assuntos
Psoríase , Dermatopatias , Administração Cutânea , Animais , Linfócitos T CD8-Positivos/metabolismo , Modelos Animais de Doenças , Imiquimode/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Psoríase/tratamento farmacológico , Psoríase/metabolismo , Pele/metabolismo , Dermatopatias/induzido quimicamente , Dermatopatias/tratamento farmacológico , Dermatopatias/metabolismo
15.
Int J Mol Sci ; 23(16)2022 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-36012178

RESUMO

Ectodysplasin A (EDA) signaling is initially identified as morphogenic signaling regulating the formation of skin appendages including teeth, hair follicles, exocrine glands in mammals, feathers in birds and scales in fish. Gene mutation in EDA signaling causes hypohidrotic ectodermal dysplasia (HED), a congenital hereditary disease with malformation of skin appendages. Interestingly, emerging evidence suggests that EDA and its receptors can modulate the proliferation, apoptosis, differentiation and migration of cancer cells, and thus may regulate tumorigenesis and cancer progression. More recently, as a newly discovered hepatocyte factor, EDA pathway has been demonstrated to be involved in the pathogenesis of nonalcoholic fatty liver disease (NAFLD) and type II diabetes by regulating glucose and lipid metabolism. In this review, we summarize the function of EDA signaling from skin appendage development to multiple other diseases, and discuss the clinical application of recombinant EDA protein as well as other potential targets for disease intervention.


Assuntos
Diabetes Mellitus Tipo 2 , Displasia Ectodérmica Anidrótica Tipo 1 , Animais , Diabetes Mellitus Tipo 2/metabolismo , Ectodisplasinas/genética , Ectodisplasinas/metabolismo , Mamíferos/metabolismo , Transdução de Sinais , Pele/metabolismo
16.
Int J Mol Sci ; 23(16)2022 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-36012184

RESUMO

Human umbilical cord lining epithelial cells [CLECs) are naïve in nature and can be ethically recovered from cords that are routinely discarded. The success of using oral mucosal epithelial cells for cornea defects hints at the feasibility of treating cutaneous wounds using non-native CLECs. Herein, we characterized CLECs using flow cytometry (FC) and skin organotypic cultures in direct comparison with skin keratinocytes (KCs). This was followed by wound healing study to compare the effects of CLEC application and the traditional use of human skin allografts (HSGs) in a porcine wound model. While CLECs were found to express all the epidermal cell markers probed, the major difference between CLECs and KCs lies in the level of expression (in FC analysis) as well as in the location of expression (of the epithelium in organotypic cultures) of some of the basal cell markers probed. On the pig wounds, CLEC application promoted accelerated healing with no adverse reaction compared to HSG use. Though CLECs, like HSGs, elicited high levels of local and systemic immune responses in the animals during the first week, these effects were tapered off more quickly in the CLEC-treated group. Overall, the in vivo porcine data point to the potential of CLECs as a non-native and safe source of cells to treat cutaneous wounds.


Assuntos
Cordão Umbilical , Cicatrização , Animais , Células Epiteliais/metabolismo , Humanos , Queratinócitos , Pele/metabolismo , Suínos
17.
Front Immunol ; 13: 932090, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35967445

RESUMO

Mast cells occupy a unique niche within tissues as long lived perpetrators of IgE mediated hypersensitivity and anaphylaxis, as well as other immune responses. However, mast cells are not identical in different tissues and the impact of this tissue heterogeneity on the interaction with other immune cells and on defined immune responses is still unclear. In this review, we synthesize the characteristics of mast cell heterogeneity in the gut and the skin. Furthermore, we attempt to connect mast cell heterogeneity with functional diversity by exploring differences in mast cell-induced immune cell recruitment in these two model organs. The differential expression of certain receptors on mast cells of different tissues, notably tissue-specific expression patterns of integrins, complement receptors and MRGPRX2, could indicate that tissue environment-dependent factors skew mast cell-immune cell interactions, for example by regulating the expression of these receptors.


Assuntos
Anafilaxia , Mastócitos , Humanos , Proteínas do Tecido Nervoso , Receptores de Complemento , Receptores Acoplados a Proteínas G , Receptores de Neuropeptídeos , Pele/metabolismo
18.
Oxid Med Cell Longev ; 2022: 4392256, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35979399

RESUMO

The regulation of collagen synthesis, which occurs in fibroblasts in the dermal layer, is a key process in dermis regeneration and skin reconstruction. Herein, we investigated whether Aronia melanocarpa extract affects the human skin condition. We focused on type I collagen synthesis using two different types of model systems: a monolayer of cells and a bioprinted 3D dermal equivalent. The Aronia extract showed no cytotoxicity and increased cell proliferation in neonatal human dermal fibroblasts. Treatment with Aronia extract increased the transcription of COL1A1 mRNA in direct proportion to the extract concentration without causing a decrease in COL1A1 mRNA degradation. Additionally, the Aronia extract inhibited the expression of MMP1 and MMP3, and an increase in type I collagen was observed along with a decrease in MMP1 protein. We also fabricated dermal equivalents from type I collagen (the major component of the dermis) and dermal fibroblasts by bioprinting. In the 3D dermis model, the compressive modulus directly affected by collagen synthesis increased in direct proportion to the Aronia extract concentration, and expression levels of MMP1 and MMP3 decreased in exactly inverse proportion to its concentration. The findings that the Aronia extract increases synthesis of type I collagen and decreases MMP1 and MMP3 expression suggest that this extract may be useful for the treatment of damaged or aged skin.


Assuntos
Metaloproteinase 1 da Matriz , Photinia , Idoso , Células Cultivadas , Colágeno Tipo I/metabolismo , Fibroblastos/metabolismo , Humanos , Recém-Nascido , Metaloproteinase 1 da Matriz/genética , Metaloproteinase 1 da Matriz/metabolismo , Metaloproteinase 3 da Matriz/metabolismo , Photinia/metabolismo , Pele/metabolismo
19.
Sci Rep ; 12(1): 13629, 2022 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-35948745

RESUMO

Atopic dermatitis is a common skin disease characterized by loss of skin integrity. Risk and severity have been associated with genetic variation especially with respect to the filaggrin gene, suggesting the importance of skin barrier function in atopic dermatitis pathogenesis. The keratin protein plays a role in epithelial health but its relationship with disease severity would benefit from further exploration. In this study, we evaluate the association between common keratin 6 variants and severity of atopic dermatitis over time using a Bayesian generalized linear mixed model to account for repeated measures. We identify groups of variants within which individual variants have similar effects on skin repair. Further assessment of the biological mechanisms by which these contribute to repair of epidermis may inform treatment of atopic dermatitis.


Assuntos
Dermatite Atópica , Teorema de Bayes , Dermatite Atópica/patologia , Predisposição Genética para Doença , Humanos , Proteínas de Filamentos Intermediários/genética , Proteínas de Filamentos Intermediários/metabolismo , Queratina-6/genética , Pele/metabolismo
20.
J Zhejiang Univ Sci B ; 23(8): 699-704, 2022 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-35953762

RESUMO

Keloids are a common type of pathological scar as a result of skin healing, which are extremely difficult to prevent and treat without recurrence. The pathological mechanism of keloids is the excessive proliferation of fibroblasts, which synthesize more extracellular matrices (ECMs), including type I/III collagen (COL-1/3), mucopolysaccharides, connective tissue growth factor (CTGF, also known as cellular communication network factor 2 (CCN2)), and fibronectin (FN) in scar tissue, mostly through the abnormal activation of transforming growth factor-|ß (TGF-|ß)/Smads pathway (Finnson et al., 2013; Song et al., 2018). Genetic factors, including race and skin tone, are considered to contribute to keloid formation. The reported incidence of keloids in black people is as high as 16%, whereas white people are less affected. The prevalence ratio of colored people to white people is 5:1||-||15:1 (Rockwell et al., 1989; LaRanger et al., 2019). In addition, keloids have not been reported in albinism patients of any race, and those with darker skin in the same race are more likely to develop this disease (LaRanger et al., 2019). Skin melanocyte activity is significantly different among people with different skin tones. The more active the melanocyte function, the more melanin is produced and the darker the skin. Similarly, in the same individual, the incidence of keloids increases during periods when melanocytes are active, such as adolescence and pregnancy. Keloids rarely appear in areas where melanocytes synthesize less melanin, such as in the palms and soles. Thus, the formation of keloids seems to be closely related to melanocyte activity.


Assuntos
Exossomos , Queloide , Adolescente , Células Cultivadas , Exossomos/metabolismo , Fibroblastos/metabolismo , Humanos , Queloide/patologia , Melaninas/metabolismo , Melanócitos/metabolismo , Melanócitos/patologia , Projetos Piloto , Pele/metabolismo , Fator de Crescimento Transformador beta/metabolismo
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