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1.
Zhongguo Zhong Yao Za Zhi ; 44(14): 2996-3001, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31602845

RESUMO

Through the records of herbal and market investigations,the samples collected from different market and origin were analyzed,and the appearance character indexes were determined in order to revise the commodity specification and grade standard of Dipsaci Radix combined with production practice. This is also to analyze association of the appearance with quality different of intrinsic components. The investigation results indicated the root's long and thick was better,and atrovirens of fracture surface,there are most of the market is dominated by the ungraded goods. Through principal component analysis,variance analysis and cluster analysis,combining with the feasibility of actual operation and herbal record,the length,middle diameter and phloem color were filtrated and the specification was divided into 2 ranks: the selection and ungraded goods,and the grade was divided into 2 ranks for the selection goods: big,small selected goods. Moreover,there were no significant correlation between the appearance and the intrinsic quality index of Dipsaci Radix,the content of extractum were significantly positive correlation with the moisture,but had extremely negative correlation with the total ash. Multiple comparisons indicated that the content of the moisture,extractum and asperosaponin VI of the selected goods were higher than the ungraded goods,while the total ash content were lower,and they hasn't significant difference. The commodity specification and grade standard of Dipsaci Radix as a basis provide commodity specification and grade standard of communities and standardizing market trade order.


Assuntos
Dipsacaceae/química , Medicamentos de Ervas Chinesas/normas , Raízes de Plantas/química , Análise por Conglomerados , Plantas Medicinais/química , Análise de Componente Principal
2.
Zhongguo Zhong Yao Za Zhi ; 44(14): 3010-3014, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31602847

RESUMO

The Dao-di herbs are recognized as high-quality Chinese medicines with good medicinal properties. The factors of their growth environment affects the quality of Dao-di herbs. Choosing the right place to produce,rationally planting appropriate local medicinal materials,and improving the quality and yield of authentic medicinal materials are prerequisites for ensuring the steady development of Chinese medicine industry. In this paper,a knowledge model of the relationship between organic medicinal materials and growth environment factors was constructed by integrating the dataset of environmental factors of medicinal materials,and a knowledge base was constructed to provide a decision for the study of suitable medicinal materials under different environmental conditions. Based on this,it is expected to provide a platform for further research on the relationship between the medicinal properties of medicinal herbs and environmental factors.


Assuntos
Asteraceae/crescimento & desenvolvimento , Bases de Dados Factuais , Ecossistema , Plantas Medicinais/crescimento & desenvolvimento , Medicamentos de Ervas Chinesas , Medicina Tradicional Chinesa
3.
Zhongguo Zhong Yao Za Zhi ; 44(14): 3094-3099, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31602858

RESUMO

To evaluate the safety of heavy metals contaminated Astragalus membranaceus,an appropriate protocol was established to study the heavy metals pollution level by health risk assessment. This study provided a detailed procedure to assess the medicinal herbs in quality control and safety evaluation,and expected to create awareness among the public on the safety of consuming of A. membranaceus or any other kinds of medicinal herbs. The heavy metals content of Cu,As,Cd,Pb and Hg in a total of 45 batches of A. membranaceus were carefully analyzed with a developed inductively coupled plasma mass spectrometry( ICP-MS). Besides,the heavy metal contamination level was further evaluated through 4 main assessment parameters,including maximum residue limit( MRL) set by International Standard Organization,estimated daily intake( EDI) set by IUPAC,target hazard quotients( THQ) and Total THQ set by USEPA and total THQs in raw herbs of A. membranaceus. In addition,the recommended MRLs of 5 main heavy metals aimed to A. membranaceus were calculated based on the regulated consumption quantity. The result showed that,under the ISO international standard of Chinese medicine-Chinese herbal medicine heavy metals,the unqualified rate was 8. 89% for A. membranaceus,which including 4 batches of A. membranaceus exceeded the MRL of As. Here,the standard THQ value of A. membranaceus was firstly proposed as 0. 02 and 0. 011 25 for adults and children,respectively,which were calculated with the recommended consumption quantity of 30 g and 9 g for adults and children. Furthermore,the values of THQ for As and total THQs in adults and children were exceeded the standard THQ in A. membranaceus,and the recommended MRLs of Pb,Cd,Hg and Cu in above medicinal materials that calculated based on health risk assessment model were higher than the regulated MRLs that set by ISO and Chinese Pharmacopeia. The research showed that the contents of heavy metals in A. membranaceus were not in the safe range and the certain non-carcinogenic risks to human body cannot be neglected. Based on above investigation result,it is easily known that the common evaluation method for raw herbs based on the comparison of MRL of heavy metals was not precise enough,and the international model of health risk assessment should be built for each medicinal herb. Above all,this study provided a more realistic research approach for safety evaluation of any other kinds of heavy metals contaminated medicinal herbs,including the establishment of heavy metals standard limit in a specified medicinal herb under recommended consumption quantity,and it is expected to create awareness among the public on the safety of consuming any other medicinal herbs.


Assuntos
Astragalus propinquus/química , Contaminação de Medicamentos , Medicamentos de Ervas Chinesas/normas , Metais Pesados/análise , Medicamentos de Ervas Chinesas/análise , Humanos , Plantas Medicinais/química , Medição de Risco
4.
Zhongguo Zhong Yao Za Zhi ; 44(15): 3151-3156, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602866

RESUMO

"Qi medicinal herbs" in China refers to a kind of regional national folk herbs related to the treatment of five labors and seven injuries,the last word of which is "Qi". Our study is to sort out and standardize the name and basic confused varieties through the establishment of " Qi medicinal herbs" VFP information database. " Qi medicinal herbs" variety sorting model of " literature research-variety survey-data mining-spatial distribution" was developed by means of literature analysis which the names and varieties of " Qi medicinal herbs" in the literature were summarized and sorted out. The relationship between the distribution of " Qi medicinal herbs" resources and the use of ethnic groups were visualized by Cytoscape 2. 8. 0 software. The information database of " Qi medicinal herbs" involved in 230 kinds of medicinal materials which including 211 species of plants( including varieties) from 66 families. Medicinal materials standard in China have 9 kinds of " Qi medicinal herbs". Among them,there are 31 kinds of " Qi medicinal herbs" with the confusion of " the different names of the same" and " the different substance of the same names". The most used ethnic groups are Tujia,Qiang and Miao. The main efficacy is clearing heat and detoxification,dispelling wind and removing dampness,etc.,and the main treatment is for injury,rheumatic arthralgia and so on. Names and varieties of " Qi medicinal herbs" among Chinese ethnic groups and folk are standardized and sorted out,which is served to promotethe " Qi medicinal herbs" reasonable protection and utilization of resources,and provide effective reference for exploring the information technology and geographical distribution of ethnic medicine and standardizing clinical medication.


Assuntos
Medicamentos de Ervas Chinesas/normas , Plantas Medicinais/classificação , China , Humanos , Medicina Tradicional Chinesa , Qi , Terminologia como Assunto
5.
Zhongguo Zhong Yao Za Zhi ; 44(15): 3203-3212, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602873

RESUMO

In order to provide guidance for the protection and utilization of resources,quality control and breeding of improved varieties,we compared the main phenotypic characters and quality of wild and transplanted Paris polyphylla var. yunnanensis collected from different producing areas. Seven phenotypic characters of 33 samples of P. polyphylla var. yunnanensis collected from Yunnan,Guizhou and Sichuan were determined by conventional methods,and the principal component analysis and cluster analysis were used to analyze the diversity of the samples. The parissaponin( polyphyllin Ⅰ,Ⅱ,Ⅵ,Ⅶ) content of the samples were detected by HPLC,and analyzed by cluster analysis. Correlation analysis of the phenotypic characters and the parissaponin content was performed. There were significant differences in seven phenotypic characters between wild and transplanted samples of P. polyphylla var. yunnanensis from different habitats,with high phenotypic diversity and abundant genetic variation. The results of principal component analysis showed that leaf shape index was the main factor of morphological variation of P. polyphylla var. yunnanensis. Cluster analysis showed that the phenotypic characters of wild and transplanted P. polyphylla var. yunnanensis could not be completely separated. The content of saponins in wild and transplanted samples from different habitats was quite different. Saponins content of 93. 94% samples met the criterion of Chinese Pharmacopoeia 2015 edition,and the overall quality was relatively steady. The results of independent sample t-test showed that there was no significant difference of all the active ingredient between wild and transplanted samples,and it couldn't be used to distinguish between wild and transplanted samples. It is the same as the results of cluster analysis. The results of correlation analysis showed that the phenotypic traits of P. polyphylla var. yunnanensis were correlated with its medicine quality,and the total content of saponins was positively correlated with leaf length and leaf shape index( r = 0. 389,0. 441; P<0. 05). Yunnan,Guizhou and Sichuan are suitable for the growth of P. polyphylla var. yunnanensis. And the transplaned P. polyphylla var. yunnanensis can be used as the same as the wild ones completely. The results provide reference for the protection and selective breeding of P. polyphylla var. yunnanensis.


Assuntos
Melanthiaceae/química , Compostos Fitoquímicos/análise , Saponinas/análise , China , Cromatografia Líquida de Alta Pressão , Ecossistema , Melhoramento Vegetal , Folhas de Planta , Plantas Medicinais/química
6.
Zhongguo Zhong Yao Za Zhi ; 44(15): 3253-3260, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602880

RESUMO

Flavonoids are a group of secondary metabolites found in plants. They have many pharmacological functions and play an important role in Chinese sumac( Rhus chinensis),which is a well-known traditional Chinese medicinal plant. Chalcone isomerase( CHI,EC 5. 5. 1. 6) is one of the key enzymes in the flavonoids biosynthesis pathway. In this paper,the full-length c DNA sequence encoding the chalcone isomerase from R. chinensis( designated as Rc CHI) was cloned by RT-PCR and rapid-amplification of c DNA Ends( RACE). The Rc CHI c DNA sequence was 1 058 bp and the open reading frame( ORF) was 738 bp. The ORF predicted to encode a 245-amino acid polypeptide. Rc CHI gene contained an intron and two exons. The sequence alignments revealed Rc CHI shared47. 1%-71. 6% identity with the homologues in other plants. Real-time PCR analysis showed that the total flavonoid levels were positively correlated with tissue-specific expressions of Rc CHI mRNA in different tissues. The recombinant protein was successfully expressed in an Escherichia coli strain with the p GEX-6 P-1 vector. In this paper,the CHI gene was cloned and characterized in the family of Anacardiaceae and will help us to obtain better knowledge of the flavonoids biosynthesis of the flavonoid compounds in R. chinensis.


Assuntos
Flavonoides/biossíntese , Liases Intramoleculares/genética , Rhus/enzimologia , Clonagem Molecular , DNA Complementar , Plantas Medicinais/enzimologia , Plantas Medicinais/genética , Rhus/genética
7.
Zhongguo Zhong Yao Za Zhi ; 44(17): 3633-3636, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31602934

RESUMO

As a treasure of traditional Chinese medicine culture,Dao-di herbs are famous for their high quality and good effect.However,traditional characteristics of Dao-di herbs and their producing areas are mostly confined to qualitative description,lacking objective evaluation indicators. Numerical taxonomy,which uses mathematical methods to improve the research of taxonomy from qualitative description to quantitative comprehensive analysis,is objective and accurate,so is widely used in the field of biology and medicine.This paper reviews the concept and main research fields of numerical taxonomy,and summarizes its application status and development prospects in the study of Dao-di herbs and characteristics of production areas,in order to lay the foundation for the objectification and standardization of Dao-di herbs.


Assuntos
Medicamentos de Ervas Chinesas/classificação , Plantas Medicinais/classificação , Medicamentos de Ervas Chinesas/normas , Medicina Tradicional Chinesa
8.
Rev. Pesqui. (Univ. Fed. Estado Rio J., Online) ; 11(5): 1129-1134, out.-dez. 2019. ilus
Artigo em Inglês, Português | LILACS, BDENF - Enfermagem | ID: biblio-1021911

RESUMO

Objetivo: Identificar as plantas utilizadas com fins terapêuticos por usuários de uma unidade pré-hospitalar pública do município de Campos dos Goytacazes/RJ, os modos de preparo e uso, e o conhecimento deles sobre às propriedades terapêuticas, mecanismos de ação e indicação das plantas que utilizam. Métodos: Pesquisa descritiva, exploratória e qualitativa, operacionalizada por dez entrevistas semiestruturadas em abril de 2016, cujos dados foram tratados por análise temática. Resultados: Foram citadas 40 plantas, sendo a mais frequente a erva cidreira (Lippia alba), utilizada como calmante. A principal forma de obtenção das plantas é por meio de cultivo próprio; e o chá, preparado por infusão ou decocção, a principal forma de consumo. Foram citados malefícios associados ao uso da arnica (Solidago chilensis). Conclusão: Verificou-se que a maior parte das plantas utilizadas é de procedência regional, cujo conhecimento de uso foi adquirido por mães e avós


Objective: The study's purpose has been to identify the plants used for therapeutic purposes by users of a public pre-hospital unit in the Campos dos Goytacazes city, Rio de Janeiro State. Furthermore, to identify the methods of preparation and use, and their knowledge about the therapeutic properties, mechanisms of action and indication of the plants they use. Methods: It is a descriptive-exploratory research with a qualitative approach, which was performed through ten semi-structured interviews in April 2016, whose data were processed by thematic analysis. Results: 40 plants were mentioned, the most frequent being the lemongrass (Lippia alba) that was used as a soothing medicine; the main way of obtaining the plants is by means of own cultivation; and the tea, prepared by infusion or decoction, the main form of consumption. Unpleasant effects associated with the use of bush arnica (Solidago chilensis) were mentioned. Conclusion: It was verified that most of the plants used are of regional origin, whose knowledge of use was acquired through their relatives


Objetivo: Identificar las plantas utilizadas con fines terapéuticos por los usuarios de una unidad pública prehospitalaria en la ciudad de Campos dos Goytacazes/RJ, los métodos de preparación, uso y sus conocimientos sobre las propiedades terapéuticas, mecanismos de acción e indicación de las plantas. Método: Se desarrolló una investigación descriptiva, exploratoria y cualitativa, operada por diez entrevistas semiestructuradas en abril de 2016, cuyos datos fueron tratados por análisis temático. Resultados: Se mencionaron 40 plantas, siendo la más frecuente la hierba de limón (Lippia alba). La forma principal de obtención de las plantas es mediante el cultivo propio; y el té, preparado por infusión o decocción, la forma principal de consumo. Se citaron los efectos de la enfermedad asociados con el uso de arnica (Solidago chilensis). Conclusión: La mayoría de las plantas utilizadas son de origen regional, cuyo conocimiento de uso fue adquirido por familiares


Assuntos
Humanos , Plantas Medicinais , Terapias Complementares , Conhecimentos, Atitudes e Prática em Saúde , Fitoterapia , Etnobotânica
9.
J Agric Food Chem ; 67(38): 10660-10666, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31479263

RESUMO

Two new indolyl diketopiperazines, gartryprostatins A and B (1 and 2), with an unusual 2,3-furan-fused pyrano[2,3-g]pyrrolo[1″,2″:4',5']pyrazino[1',2':1,5]pyrrolo[2,3-b]indole nucleus, along with a new naturally occurring compound (gartryprostatin C, 3) were isolated from the solid culture of Aspergillus sp. GZWMJZ-258, an endophyte from Garcinia multiflora (Guttiferae). The structures of compounds 1-3 were determined by nuclear magnetic resonance, mass spectrometry, Marfey's analysis of amino acids, and chemical calculation. Compounds 1-3 displayed selective inhibition on human FLT3-ITD mutant AML cell line, MV4-11, with IC50 values of 7.2, 10.0, and 0.22 µM, respectively.


Assuntos
Aspergillus/química , Dicetopiperazinas/farmacologia , Endófitos/química , Garcinia/microbiologia , Plantas Medicinais/microbiologia , Aspergillus/genética , Aspergillus/isolamento & purificação , Aspergillus/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Dicetopiperazinas/química , Dicetopiperazinas/metabolismo , Endófitos/genética , Endófitos/isolamento & purificação , Endófitos/metabolismo , Humanos , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Estrutura Molecular
10.
Acta Virol ; 63(3): 316-321, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31507198

RESUMO

The recent Zika virus (ZIKV) outbreaks and rapid spread in tropical Latin America since introduction to Brazil in 2014, and now appearing cases in the USA, are alarming. World Health Organization (WHO) has considered transmission of ZIKV, a serious public health problem because of the increasing number of outbreaks. There are currently no drugs approved for the treatment of ZIKV infection. Discovery of safe and effective drugs are hampered by the risk in treating pregnant woman and toxicity to the fetus. Sweet basil, known as Ocimum basilicum in the scientific community, is a very well-known medicinal herb. Numerous studies have documented its beneficial activity against a great variety of human pathogens ranging from bacteria and virus to fungus and protozoans. Although, basil extracts and oils have been tested successfully against other viruses, its application to tackle ZIKV infection has not been exploited at all. In this study, we report for the first time that highly diluted ethanol extracts prepared from basil leaves can effectively inhibit ZIKV replication in Vero E6 cells with a half maximal inhibitory concentration (IC50) value of 1:134. The diluted extract as well as the amount of ethanol that goes into its preparation have been found to be completely non-toxic to the above mentioned cell line. The extract seems to inhibit the virus at the step of attachment and entry into the host cell. The specific inhibition of ZIKV observed using the basil leaf extract suggests a new alternative mode of treatment against flavivirus. Keywords: Zika virus; basil extract; antiviral.


Assuntos
Ocimum basilicum , Extratos Vegetais , Internalização do Vírus , Infecção por Zika virus , Animais , Sobrevivência Celular/efeitos dos fármacos , Cercopithecus aethiops , Etanol/química , Ocimum basilicum/química , Extratos Vegetais/farmacologia , Folhas de Planta/química , Plantas Medicinais/química , Células Vero , Internalização do Vírus/efeitos dos fármacos , Zika virus/efeitos dos fármacos , Zika virus/fisiologia
11.
Medicine (Baltimore) ; 98(37): e17005, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31517818

RESUMO

BACKGROUND: Overactive bladder (OAB) is a common disease in the urinary system. The morbidity is increasing annually. Herbal medicines have been pervasively used in the therapy of OAB. However, systematic review or meta-analysis has not been found to assess the effects and safety of herbal medicines in curing OAB at present. Hence, the systematic review is conducted to scientifically and methodically evaluate the value of its effectiveness and safety of herbal medicines on OAB. METHODS: We will collect all randomized controlled trials (RCTs) to assess the effectiveness and safety of herbal medicines on OAB. The RCTs will be searched from the electronic databases, including 7 English databases, consisting of PubMed, Excerpta Medica Database (EMBASE), MEDLINE, Web of Science, Cochrane Library, SpringerLink, and WHO International Clinical Trials Registry Platform (ICTRP), and 4 Chinese databases, namely Wanfang Chinese database, China National Knowledge Infrastructure (CNKI), Chinese Biomedical Literature Database (CBM), and Chinese Scientific Journal Database (VIP), others valid search strategy will be adopted. All the RCTs will be assessed from the databases establishment to July 2019. We will perform the meta-analysis of RCTs when the dissertation is appropriate. We will conduct an assessment including data synthesis, bias risk, and subgroup analysis by means of Review Manager software (RevMan) V.5.3.5 software while the setting condition is met. RESULTS: This systematic view and meta-analysis will generate a summary based on the current relevant research to calculate the effects and safety of herbal medicines in promoting the therapy of OAB. Furthermore, it will provide a high-quality synthesis for participants who use herbal medicine to cure OAB. CONCLUSION: The summary of our systematic view will verify whether herbal medicines intervention could be an effective and safety approach in curing OAB.


Assuntos
Metanálise como Assunto , Fitoterapia , Revisão Sistemática como Assunto , Bexiga Urinária Hiperativa/tratamento farmacológico , Humanos , Fitoterapia/efeitos adversos , Plantas Medicinais , Ensaios Clínicos Controlados Aleatórios como Assunto
12.
Fitoterapia ; 137: 104282, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31381956

RESUMO

Four new polyketides, alternatains A-D (1-4), along with 17 known compounds (5-21) were obtained from the solid substrate fermentation cultures of Alternaria alternata MT-47, an endophytic fungus isolated from the medicinal plant of Huperzia serrata. Their structures were elucidated by extensive spectroscopic and spectrometric techniques (1D and 2D NMR, IR, and HRESIMS) and calculated electronic circular dichroism (ECD) method. Compounds 4, 6, 15, and 21 exhibited inhibitory activities on ATP release of thrombin-activated platelets with IC50 values in the range of 18.2-68.8 µM.


Assuntos
Alternaria/química , Anticoagulantes/farmacologia , Plaquetas/efeitos dos fármacos , Huperzia/microbiologia , Policetídeos/farmacologia , Acetilcolinesterase , Trifosfato de Adenosina , Anticoagulantes/isolamento & purificação , Butirilcolinesterase , China , Inibidores da Colinesterase , Endófitos/química , Humanos , Estrutura Molecular , Plantas Medicinais/microbiologia , Policetídeos/isolamento & purificação
13.
Fitoterapia ; 137: 104285, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31386897

RESUMO

Botanical-based natural products are an important resource for medicinal drug discovery and continue to provide diverse pharmacophores with therapeutic potential against cancer and other human diseases. A prototype Traditional Chinese Medicine (TCM) plant extract library has been established at the US National Cancer Institute, which contains both the organic and aqueous extracts of 132 authenticated medicinal plant species that collectively represent the potential therapeutic contents of most commonly used TCM herbal prescriptions. This library is publicly available in 96- and 384- well plates for high throughput screening across a broad array of biological targets, as well as in larger quantities for isolation of active chemical ingredients. Herein, we present the methodology used to generate the library and the preliminary assessment of the anti-proliferative activity of this crude extract library in NCI-60 human cancer cell lines screen. Particularly, we report the chemical profiling and metabolome comparison analysis of four commonly used TCM plants, namely Brucea javanica, Dioscorea nipponica, Cynanchum atratum, and Salvia miltiorrhiza. Bioassay-guided isolation resulted in the identification of the active compounds, and different extraction methods were compared for their abilities to extract cytotoxic compounds and to concentrate biologically active natural products.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/química , Plantas Medicinais/química , Antineoplásicos Fitogênicos/isolamento & purificação , Brucea/química , Linhagem Celular Tumoral , China , Cynanchum/química , Dioscorea/química , Descoberta de Drogas , Humanos , Medicina Tradicional Chinesa , National Cancer Institute (U.S.) , Compostos Fitoquímicos/isolamento & purificação , Salvia miltiorrhiza/química , Estados Unidos
14.
Stud Health Technol Inform ; 264: 278-282, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31437929

RESUMO

Chemicals derived from plants (phytochemicals) are major concepts of interest in the study of medicinal plants. To date, efforts to catalogue and organize phytochemical knowledge have resorted to manual approaches. This study explored the potential to leverage publicly accessible semantic knowledge sources for identifying possible phytochemicals. Within the context of this feasibility study, putative phytochemicals were identified for more than 4,000 plants from the Medical Subject Headings Supplementary Concept Records and the Semantic MEDLINE Database. An examination of phytochemicals identified for five selected plant species using the method developed here reveals that there is a disparity in electronically catalogued phytochemical knowledge compared to information from Dr. Duke's Phytochemical and Ethnobotanical Databases maintained by the United States Department of Agriculture. The results therefore suggest that semantic knowledge sources for biomedicine can be utilized as a source for identifying potential phytochemicals and thus contribute to the overall curation of plant phytochemical knowledge.


Assuntos
Plantas Medicinais , Semântica , Bases de Dados Factuais , Compostos Fitoquímicos
15.
Stud Health Technol Inform ; 264: 1548-1549, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31438225

RESUMO

The purpose of this study is to describe the design and development of the first release of the West African Herbal based Traditional Medicine Knowledge Graph (WATRIMed). It is a resource containing Traditional Medicine (TM) related entities and linked with publicly available knowledge bases in order to facilitate bringing West African TM into the digital world. The core model comprises currently 556 concepts including 143 identified West African medicinal plants and 108 recipes used by tradi-practitioners to treat 110 diseases and symptoms which are commonly encountered in this part of the world.


Assuntos
Plantas Medicinais , Conhecimento , Medicina Tradicional Africana , Reconhecimento Automatizado de Padrão , Fitoterapia
16.
An Acad Bras Cienc ; 91(suppl 3): e20190208, 2019 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-31411242

RESUMO

The development of our society has been based on the use of biodiversity, especially for nutrition, medicines and beauty. Brazil is the nation with the largest biodiversity in the world, with a rich chemical diversity, which is a potential source for bioeconomy. Considering the chemical and biological diversity of the Brazilian territory, we would like to highlight the value of secondary metabolites from Brazilian biodiversity with potential application for new products and technologies and the importance of scientific programs to support the sustainable use of biodiversity.


Assuntos
Biodiversidade , Produtos Biológicos , Descoberta de Drogas , Farmacoeconomia , Plantas Medicinais/química , Brasil , Plantas Medicinais/classificação
17.
Int J Nanomedicine ; 14: 5303-5321, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31406461

RESUMO

Metabolic syndrome is a common metabolic disorder which has become a public health challenge worldwide. There has been growing interest in medications including natural products as complementary or alternative choices for common chemical therapeutics regarding their limited side effects and ease of access. Nanosizing these compounds may help to increase their solubility, bioavailability, and promisingly enhance their efficacy. This study, for the first time, provides a comprehensive overview of the application of natural-products-based nanoformulations in the management of metabolic syndrome. Different phytochemicals including curcumin, berberine, Capsicum oleoresin, naringenin, emodin, gymnemic acid, resveratrol, quercetin, scutellarin, stevioside, silybin, baicalin, and others have been nanosized hitherto, and their nanosizing method and effect in treatment and alleviating metabolic syndrome have been reviewed and discussed in this study. It has been discovered that there are several pathways or molecular targets relevant to metabolic disorders which are affected by these compounds. Various natural-based nanoformulations have shown promising effect in treatment of metabolic syndrome, and therefore can be considered as future candidates instead of or in conjunction with pharmaceutical drugs if they pass clinical trials successfully.


Assuntos
Produtos Biológicos/uso terapêutico , Composição de Medicamentos , Síndrome Metabólica/tratamento farmacológico , Nanopartículas/química , Humanos , Síndrome Metabólica/fisiopatologia , Plantas Medicinais/química , Pesquisa Médica Translacional
18.
Zhongguo Zhong Yao Za Zhi ; 44(12): 2421-2432, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31359707

RESUMO

With the development of various biotechnology,the research on molecular genetics of medicinal plants has gradually deepened. In this paper,the research system of molecular genetics of medicinal plants was proposed for the first time,which was elaborated from the aspects of genetic resources,genome,gene function and research methods. The application fields of medicinal plant mainly contain species identification,molecular breeding and biosynthesis. The research directions of molecular genetics of medicinal plants in genetic resources,model platform,synthetic biology and molecular breeding were put forward,which include 1 000 genome projects of medicinal plants,model species and mutant libraries,gene original libraries of heterologous synthetic systems,construction gene original library and specific chassis cells in heterologous synthesis system of active ingredient,breeding of new varieties of medicinal plants with high active ingredient and high resistance based on molecular markers andtransgenes.


Assuntos
Biologia Molecular/tendências , Plantas Medicinais/genética , Biotecnologia , Biblioteca Gênica , Marcadores Genéticos , Genoma de Planta , Melhoramento Vegetal , Pesquisa , Transgenes
19.
Zhongguo Zhong Yao Za Zhi ; 44(12): 2580-2587, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31359727

RESUMO

Panax japonicus is a traditional Chinese medicine,and its principle components have shown certain pharmacological activities for cell damage,aging and cell apoptosis. In order to clarify the pharmacological mechanism and involved metabolic pathways of P. japonicas,the gene expression of Tetrahymena thermophila under P. japonicus treatment was analyzed through high-throughput transcriptome sequencing in this study. Based on the transcriptome analysis,3 544 differentially expressed genes were identified in control group,of which 1 945 genes showed up-regulated expression and 1 599 genes showed down-regulated expression. Under P. japonicas treatment in the experiment group,3 312 differentially expressed genes were screened,of which 1 `493 genes showed up-regulated expression and 1 819 genes showed down-regulated expression. GO enrichment analysis indicated that in control group,the genes in the cells in a series of fundamental biological process were down-regulated,such as DNA replication and protein synthesis; while the signal transduction process and fatty acids oxidizing process were enriched. Whereas in the experiment group,down-regulated genes were mainly enriched in oxidation-reduction,cofactor metabolic process and vitamin metabolic process; up-regulated genes were enriched in signal transduction process and protein modification process. In the analysis using KEGG database,cell cycle pathway was enhanced and autophagy pathway was inhibited under the condition of P. japonicas treatment. Real-time quantitative polymerase chain reaction( RT-qPCR) was used to detect the expression differences between 6 up-regulated and 4 down-regulated genes in related metabolic pathways. The RT-q PCR results and RNA-Seq data were highly correlated and consistent with each other. This study could provide important direction and basis for further study on the mechanism of cell growth regulation with the treatment of P. japonica.


Assuntos
Panax/química , Plantas Medicinais/química , Tetrahymena thermophila/efeitos dos fármacos , Tetrahymena thermophila/genética , Transcriptoma , Expressão Gênica , Perfilação da Expressão Gênica , Redes e Vias Metabólicas
20.
Zhongguo Zhong Yao Za Zhi ; 44(12): 2600-2606, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31359730

RESUMO

Books on Chinese herbal medicines have shown that Dendrobium has the effect of nourishing Yin and reinforcing Yin,usually used for constipation induced by spleen Yin deficiency in clinical application. D. huoshanense,as an independent species among many species of Dendrobium,has no experimental studies about its effects on spleen Yin deficiency-type constipation. The purpose of this experiment was to illustrate the therapeutic effect of D. huoshanense on the constipation of spleen Yin deficiency type in rats,investigate its preliminary mechanism,and compare it with the D. officinale and D. nobile contained in the Chinese Pharmacopoeia to clarify its characteristics. The spleen Yin deficiency model was replicated in 70 rats by the composite factor method,and then the model rats were randomly divided into 7 groups: model group,Liuwei Dihuang Pills group( LWDHP),D. huoshanense high( DHS-H),medium( DHS-M),low( DHS-L) dose groups,D. nobile group( DNS),and D. officinale group( DOS),and another 10 rats were used as normal group( Normal). After 7 continuous days of administration,the fecal water content and intestine propulsion rate of each group were detected. HE staining was used to observe the pathological damage of ileum and colon in each group. Immunohistochemistry and Western blot were used to detect aquaporin 3( AQP3) expressions,while the expression levels of the somatostatin( SS) and motilin( MTL) in the ileum of each group were detected by enzyme-linked immunosorbent assay. The results showed that as compared with the model group,the rats in each drug-administered group had increased number of fecal pellets,increased fecal water content,and the increased intestinal propulsion rate( P<0. 01),while the pathological damage of the ileum and colon was significantly reduced; the expression of AQP3 protein was significantly decreased( P<0. 01); the level of MTL was significantly increased and the level of SS was decreased( P<0. 01). All DHS groups showed a good dose-effect relationship,and the same dose treatment effect was equivalent to that of DOS,but it was superior to DNS. Therefore,DHS has a significant therapeutic effect on constipation of spleen Yin deficiency type,and its mechanism may be related to intestinal motility and water-liquid metabolism,with a good therapeutic effect.


Assuntos
Constipação Intestinal/tratamento farmacológico , Dendrobium/química , Medicamentos de Ervas Chinesas/farmacologia , Plantas Medicinais/química , Deficiência da Energia Yin/tratamento farmacológico , Animais , Intestinos , Distribuição Aleatória , Ratos , Baço
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