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1.
World J Microbiol Biotechnol ; 41(1): 20, 2024 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-39739081

RESUMO

Arsenic (As) contamination in agricultural groundwater and soil is a significant economic and health problem worldwide. It inhibits soybean (Glycine max (L.) Merr.) nodulation and biological nitrogen fixation in symbiosis with Bradyrhizobium japonicum E109 (E109), a commonly used rhizobial strain for commercial biofertiliser formulation in Argentina. In the context of sustainable and climate-smart agriculture promoted by FAO, co-inoculating legumes with As-tolerant plant growth-promoting bacteria (PGPB) is suggested as a superior alternative to single inoculation. This study aimed to evaluate the impact of As on plant growth-promoting (PGP) traits -siderophore and indole acetic acid production, phosphate solubilisation, diazotrophic activity and hydrolytic enzymes activity- in E109 and three other PGPB strains: Pseudomonas sp. AW4 (AW4), Bacillus pumilus SF5 (SF5) and Bacillus toyonensis SFC 500-1E (Bt). In addition, bacterial compatibility and adhesion on soybean seed were evaluated. Arsenic significantly reduced PGP traits of E109 even at low concentrations, AW4's traits remained unchanged, while those of SF5 and Bt traits were affected (positively or negatively) only at the highest concentrations tested (500 µM arsenate, 250 µM arsenite). All PGPB strains were compatible with E109 under both control and As-stress conditions. Soybean seed adhesion was reduced for E109, only under As stress. Findings suggest that the effect of As on PGP traits is highly strain-dependent and influenced by As concentration and speciation. AW4, SF5, and Bt strains show promise for co-inoculation with E109 in soybean cultivation.


Assuntos
Arsênio , Bradyrhizobium , Glycine max , Fixação de Nitrogênio , Sementes , Microbiologia do Solo , Simbiose , Glycine max/microbiologia , Glycine max/crescimento & desenvolvimento , Arsênio/metabolismo , Sementes/microbiologia , Sementes/crescimento & desenvolvimento , Bradyrhizobium/fisiologia , Bradyrhizobium/metabolismo , Bacillus/fisiologia , Bacillus/metabolismo , Aderência Bacteriana , Pseudomonas , Poluentes do Solo/metabolismo , Argentina , Fosfatos/metabolismo , Ácidos Indolacéticos/metabolismo , Nodulação
2.
World J Microbiol Biotechnol ; 41(1): 7, 2024 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-39690265

RESUMO

The association of bacteria resistant to potentially toxic metals (PTMs) with plants to remove, transfer, or stabilize these elements from the soil is an appropriate tool for phytoremediation processes in metal-contaminated environments. The objective of this study was to evaluate the potential of Rhizobium sp. LBMP-C04 for phytoremediation processes and plant growth promotion in metal-contaminated soils. Functional annotation allowed us to predict a variety of genes related to PTMs resistance and plant growth promotion in the bacterial genome. Resistance genes are mainly associated with DNA repair, and the import or export of metals in bacterial cells to maintain cell homeostasis. Genes that promote plant growth are related to mechanisms of osmotic stress tolerance, phosphate solubilization, nitrogen metabolism, biological nitrogen fixation, biofilm formation, heat shock responses, indole-3-acetic acid (IAA) biosynthesis, tryptophan, and organic acids metabolism. Biochemical tests indicated that Rhizobium sp. LBMP-C04 can solubilize calcium phosphate and produce siderophores and IAA in vitro in the presence of the PTMs Cd2+,Cu2+,Cr3+,Cr6+, Zn2+, and Ni2+. Results indicate the possibility of using Rhizobium sp. LBMP-C04 as a potentially efficient bacterium in phytoremediation processesin environments contaminated by PTMs and simultaneously promote plant growth.


Assuntos
Biodegradação Ambiental , Ácidos Indolacéticos , Desenvolvimento Vegetal , Rhizobium , Microbiologia do Solo , Poluentes do Solo , Rhizobium/genética , Rhizobium/metabolismo , Poluentes do Solo/metabolismo , Poluentes do Solo/toxicidade , Ácidos Indolacéticos/metabolismo , Metais/metabolismo , Metais Pesados/toxicidade , Metais Pesados/metabolismo , Sideróforos/metabolismo , Fosfatos/metabolismo , Genoma Bacteriano
3.
J Hazard Mater ; 480: 136301, 2024 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-39504770

RESUMO

Copper (Cu) is an essential element for plants, participating in photosynthesis, oxidative metabolism and cell wall synthesis. However, excessive Cu may become toxic, as Cu participates in Fenton chemistry and cause oxidative stress. Grapevine (Vitis sp.) is an important perennial crop, used for in natura consumption as well as for wine and juice. Vineyards are susceptible to fungal diseases that are commonly controlled by using Cu-based fungicides, which can lead to Cu accumulation in the soil. Since grape production is based on grafting scions of consumed-friendly varieties onto rootstocks that can withstand soil-borne diseases and stresses, it is important to identify rootstock genotypes that are tolerant to Cu excess. In this work, we compared physiological and molecular responses of four Vitis sp. rootstock genotypes to Cu excess, namely IAC, IBCA, Paulsen and Isabel. While IAC, IBCA, Paulsen were similarly tolerant, Isabel was the most sensitive to Cu excess. IAC and IBCA showed higher Cu accumulation in shoots, suggesting distinct partitioning strategy. We identified core Cu excess-responsive genes in grapevine roots of all four genotypes, including a putative HMA vacuolar Cu transporter and Cu-binding proteins. Genes related to the homeostasis of other elements are altered, such as iron (Fe) and phosphorus (P), suggesting that Cu excess alters the ionome balance. IAC and IBCA had extensive changes in their laccase gene repertoire, suggesting that could be related to the distinct Cu partitioning. Moreover, genes associated specifically with Isabel could be related to the genotype Cu excess sensitivity. Our work provides a valuable dataset for understanding variation in Cu tolerance how roots respond transcriptionally to Cu stress, and provide candidate genes for engineering Cu tolerance in grapevines.


Assuntos
Cobre , Raízes de Plantas , Vitis , Vitis/genética , Vitis/metabolismo , Vitis/efeitos dos fármacos , Raízes de Plantas/metabolismo , Raízes de Plantas/genética , Raízes de Plantas/efeitos dos fármacos , Cobre/toxicidade , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Genótipo , Poluentes do Solo/toxicidade , Poluentes do Solo/metabolismo
4.
Environ Geochem Health ; 46(11): 478, 2024 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-39412703

RESUMO

Anthropogenic activities, encompassing vast agricultural and industrial operations around the world, exert substantial pressure on the environment, culminating in profound ecological impacts. These activities exacerbate soil contamination problems with pollutants such as mercury (Hg) and chlorpyrifos (CPF) that are notable for their widespread presence and detrimental effects. The objective of this study is to evaluate the phytoremediation potential of Phaseolus vulgaris L., augmented with various combinations of biochar, mycorrhizal, and compost amendments, as a sustainable alternative for the remediation of soils contaminated with Hg and CPF. For this purpose, soil from a mining area with mercury contamination has been taken, to which CPF has been added in different concentrations. Then, previously germinated Phaseolus vulgaris L. seedlings with an average height of 10 cm were planted. Electrical conductivity, pH, organic matter, CPF, and Hg, as well as seedling growth parameters, have been evaluated to determine the processes of absorption of soil contaminants into the plant. A combination of biochar with mycorrhiza has been found to be an optimal choice for CPF and Hg remediation. However, all amendments have proven to be efficient in the remediation processes of the tested contaminants.


Assuntos
Biodegradação Ambiental , Carvão Vegetal , Clorpirifos , Compostagem , Mercúrio , Micorrizas , Phaseolus , Poluentes do Solo , Carvão Vegetal/química , Phaseolus/metabolismo , Poluentes do Solo/metabolismo , Mercúrio/metabolismo , Micorrizas/metabolismo , Clorpirifos/metabolismo , Solo/química , Plântula/metabolismo
5.
Environ Sci Pollut Res Int ; 31(54): 63120-63135, 2024 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-39472373

RESUMO

Mercury (Hg) is a non-essential trace metal, toxic to living beings and complex to quantify and mitigate in the environment. In this study, 25 plant species native to an Amazon-Cerrado transition area were tested for use in Hg remediation. Species identification, Hg quantification in plant biomass and soil at each sampling point, and evaluation of Hg compartmentalization in each plant were carried out. The results were subjected to statistical tests and evaluated using translocation coefficients (FT), bioconcentration (FBC), and bioaccumulation (FB). The results demonstrated that the distribution and accumulation of Hg differed between species and between the parts of the plant evaluated. Soil was the predominant source of Hg in the study area. The study highlighted seven species with Hg phytoremediation potential. Five translocator species were characterized, among these a preferentially bioaccumulating and bioconcentrating species, in addition to a bioconcentrating species and a preferentially bioconcentrating and bioaccumulating species of Hg. Potentially accumulating species stood out, Blechnum serrulatum Rich. (Blechnaceae), Mauritia flexuosa L.f. (Arecaceae), and Montrichardia arborescens (L.) Schott (Araceae), all widely distributed in tropical regions, characterized as rooted, terrestrial, or amphibious and associated with ruderal environments.


Assuntos
Biodegradação Ambiental , Mercúrio , Poluentes do Solo , Mercúrio/metabolismo , Poluentes do Solo/metabolismo , Brasil , Plantas/metabolismo , Bioacumulação
6.
Bull Environ Contam Toxicol ; 113(4): 39, 2024 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-39242363

RESUMO

Phytoremediation is a rapidly expanding process due to its technical and economic viability. The objective of this work was to evaluate the phytoremediation potential of Helianthus annuus in three cultivation media: artificially contaminated Catalão soil, hydroponics and roadside soil. In hydroponics, ZnCl2 doses 0.32 mgL- 1, 29.94 mgL- 1, 60.06 mgL- 1, 119.94 mgL- 1 were used. While in the artificially contaminated soil, the doses were 0 mgkg- 1, 299 mgkg- 1, 599 mgkg- 1, 1498 mgkg- 1. Physiological analyzes made it possible to demonstrate that treatments T3 and T4, with the highest concentrations of the metal, inhibited growth and promoted darkening of the roots. The highest Zn contents occurred in the aerial part. The results indicated that Helianthus annuus was classified as hyperaccumulator due to its ability to accumulate high levels of Zn mainly in artificially contaminated soil.


Assuntos
Biodegradação Ambiental , Helianthus , Poluentes do Solo , Clima Tropical , Zinco , Helianthus/metabolismo , Poluentes do Solo/metabolismo , Poluentes do Solo/análise , Zinco/metabolismo , Raízes de Plantas/metabolismo , Hidroponia
7.
Environ Sci Pollut Res Int ; 31(38): 50372-50387, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-39090300

RESUMO

Potentially harmful element (PHE) bioavailability is important to environmental contamination and must be checked under several soil conditions. This study aimed to assess Fe, Mn, and PHE uptake by rice (Oryza sativa) grown on flooded and non-flooded Fe tailings collected from the Doce River basin after its collapse in Brazil. After 65 days of sowing, shoots and roots were harvested to determine PHE concentrations. The mean concentrations of Mn in shoots and Fe in the roots of rice grown on the flooded tailings were 2140 mg kg-1 and 15,219 mg kg-1, respectively. Mn was extensively translocated from roots to shoots (translocation factor (TF) = 2). Conversely, Fe accumulated in roots (TF = 0.015) and caused morphological damage to this rice organ. The application of macro and micronutrients lessened Fe toxicity in the roots of rice cultivated on the flooded tailings. The flooding of tailings influenced more Fe accumulation than Mn accumulation by rice plants. The PHE Ag, As, Cd, Ni, Hg, Pb, and Sb exhibited low total concentrations (maximum of 9 mg kg-1 for Ni and a minimum of 0.2 mg kg-1 for Cd, Hg, and Sb), and it was not observed an increase in their availability under tailings flooding conditions.


Assuntos
Ferro , Manganês , Oryza , Rios , Brasil , Ferro/metabolismo , Inundações , Poluentes do Solo/metabolismo , Monitoramento Ambiental , Raízes de Plantas/metabolismo
8.
Microbiome ; 12(1): 146, 2024 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-39113100

RESUMO

BACKGROUND: Bioaugmentation is considered a sustainable and cost-effective methodology to recover contaminated environments, but its outcome is highly variable. Predation is a key top-down control mechanism affecting inoculum establishment, however, its effects on this process have received little attention. This study focused on the impact of trophic interactions on bioaugmentation success in two soils with different pollution exposure histories. We inoculated a 13C-labelled pollutant-degrading consortium in these soils and tracked the fate of the labelled biomass through stable isotope probing (SIP) of DNA. We identified active bacterial and eukaryotic inoculum-biomass consumers through amplicon sequencing of 16S rRNA and 18S rRNA genes coupled to a novel enrichment factor calculation. RESULTS: Inoculation effectively increased PAH removal in the short-term, but not in the long-term polluted soil. A decrease in the relative abundance of the inoculated genera was observed already on day 15 in the long-term polluted soil, while growth of these genera was observed in the short-term polluted soil, indicating establishment of the inoculum. In both soils, eukaryotic genera dominated as early incorporators of 13C-labelled biomass, while bacteria incorporated the labelled biomass at the end of the incubation period, probably through cross-feeding. We also found different successional patterns between the two soils. In the short-term polluted soil, Cercozoa and Fungi genera predominated as early incorporators, whereas Ciliophora, Ochrophyta and Amoebozoa were the predominant genera in the long-term polluted soil. CONCLUSION: Our results showed differences in the inoculum establishment and predator community responses, affecting bioaugmentation efficiency. This highlights the need to further study predation effects on inoculum survival to increase the applicability of inoculation-based technologies. Video Abstract.


Assuntos
Bactérias , Biodegradação Ambiental , RNA Ribossômico 16S , RNA Ribossômico 18S , Microbiologia do Solo , Poluentes do Solo , Solo , Solo/química , Poluentes do Solo/metabolismo , RNA Ribossômico 16S/genética , RNA Ribossômico 18S/genética , Bactérias/metabolismo , Bactérias/classificação , Bactérias/genética , Biomassa , Isótopos de Carbono/metabolismo , Cadeia Alimentar , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Marcação por Isótopo
9.
Int J Phytoremediation ; 26(13): 2194-2205, 2024 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-39008081

RESUMO

Soil salinity is a significant abiotic stress and poses risks to environmental sustainability. Thus, the improvement of the time for recovering the salt-affect soil is crucial for the phytoextraction process using halophytes plants, especially regarding on nutritional management. We evaluated the responses of Atriplex nummularia Lindl. to nitrogen (N) and phosphorus (P) under different salinity levels. The treatments comprised doses of N (N1 = 80 kg ha-1) and P (P1 = 60 kg ha-1): (1) without N and P (N0P0) (control); (2) with N and without P (N1P0); (3) without N and with P (N0P1); and (4) with N and P (N1P1) and five levels of electrical conductivity from irrigation water: 0.08, 1.7, 4.8, 8.6, and 12.5 dS m-1. The. We evaluated dry biomass of leaves, stems, and roots 93 days after transplantation. We also assessed the leaf and osmotic water potential, the osmotic adjustment, and the nutrient contents (N, P, Na, and K). N application increased 22.3, 17.8, and 32.8% the leaf biomass, stem biomass, and osmotic adjustment, respectively; and consequently, boosts Na extraction in 27.8%. Thus, the time of the phytoextraction process can be improved with N fertilizer at a rate of 80 kg ha-1.


Very few studies have investigated the nutrient dynamics responses in Atriplex species in salt-affected soils; thus, this study represents a novelty. We tested the management of nitrogen (N) and phosphate (P) fertilizers to increase crop yield and optimize the phytoextraction process in salt-affected soils. We believe our results contribute to the improvement of the knowledge of this relevant topic, mainly in terms of the recovery of areas degraded by salinity. There is a paucity of studies associating salinity and nutritional management of soils worldwide.


Assuntos
Atriplex , Biodegradação Ambiental , Nitrogênio , Fósforo , Poluentes do Solo , Solo , Atriplex/fisiologia , Fósforo/metabolismo , Nitrogênio/metabolismo , Poluentes do Solo/metabolismo , Solo/química , Salinidade , Plantas Tolerantes a Sal/fisiologia , Folhas de Planta , Fertilizantes
10.
Int J Phytoremediation ; 26(13): 2175-2182, 2024 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-39010720

RESUMO

The objective of this study was to determine the efficiency of the microbial rhizosphere (Canavalia ensiformis) in the phytoremediation of sulfentrazone using quantification methods (CO2 evolution, microbial biomass carbon, and metabolic quotient) and identification of bacteria (PCR-DGGE technique). The experiment was conducted in a completely randomized design, in a 2x4 factorial scheme, with four replications. The treatments were composed of rhizospheric soil (cultivated with C. ensiformis) and non-rhizosphere soil (uncultivated soil); and four levels of contamination by sulfentrazone (0, 200, 400, and 800 g ha-1 a.i.). The microbiota associated with the rhizosphere of C. ensiformis efficiently reduced sulfentrazone residues in the soil, with better performance at the dose of 200 g ha-1 a.i. Using the PCR-DGGE technique allowed the distinction of two profiles of bacteria in the rhizospheric activity of C. ensiformis. The second bacterial profile formed was more efficient in decontaminating soil contaminated with sulfentrazone residue. The microbiota associated with the rhizosphere of C. ensiformis has an efficient profile in decontaminating soils with residues equivalent to 200 g ha-1 a.i. the herbicide sulfentrazone.


Phytoremediation of soils contaminated with herbicide residues is a viable technique for decontamination of the environment.Canavalia ensiformis has an efficient profile in the decontamination of soils with residue equivalent to 200 g ha−1 a.i. of the herbicide sulfentrazone.The PCR technique and microbial respiration used to analyze the diversity and estimate the bacterial population of a soil are viable tools to evaluate the phytoremediation potential of the microbiota associated with plant species.


Assuntos
Biodegradação Ambiental , Microbiota , Rizosfera , Microbiologia do Solo , Poluentes do Solo , Poluentes do Solo/metabolismo , Triazóis , Bactérias/metabolismo , Herbicidas/metabolismo
11.
Environ Sci Pollut Res Int ; 31(34): 47116-47131, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38985418

RESUMO

Mining is a major economic activity in many developing countries. However, it disturbs the environment, producing enormous quantities of waste, known as mine tailings, which can have deleterious environmental impact, due to their high heavy metals (HM) content. Often, foundation species that establish on mine tailings are good candidates to study the effects of HM bioaccumulation at different levels of biological organization. Prosopis laevigata is considered a HM hyperaccumulator which presents attributes of a foundation species (FS) and establishes naturally on mine tailings. We evaluated the bioaccumulation of Cu, Pb, and Zn in P. laevigata foliar tissue, the leaf micro- and macro-morphological characters, DNA damage, and population genetic effects. In total, 80 P. laevigata individuals (20/site) belonging to four populations: The individuals from both sites (exposed and reference) bioaccumulated HMs (Pb > Cu > Zn). However, in the exposed individuals, Pb and Cu bioaccumulation was significantly higher. Also, a significant effect of macro- and micro-morphological characters was registered, showing significantly lower values in individuals from the exposed sites. In addition, we found significant differences in genotoxic damage in P. laevigata individuals, between the exposed and reference sites. In contrast, for the micro-morphological characters, none of the analyzed metals had any influence. P. laevigata did not show significant differences in the genetic structure and diversity between exposed and reference populations. However, four haplotypes and four private alleles were found in the exposed populations. Since P. laevigata is a species that establishes naturally in polluted sites and bioaccumulates HM in its foliar tissues, the resulting genetic, individual and population effects have not been severe enough to show detrimental effects; hence, P. laevigata can be a useful tool in phytoremediation strategies for soils polluted with Pb and Cu, maintaining its important ecological functions.


Assuntos
Bioacumulação , Metais Pesados , Prosopis , Poluentes do Solo , Metais Pesados/metabolismo , Prosopis/metabolismo , Poluentes do Solo/metabolismo , Biomarcadores/metabolismo , Mineração , Monitoramento Ambiental/métodos
12.
Int J Phytoremediation ; 26(13): 2084-2102, 2024 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-38975678

RESUMO

This article seeks to evaluate the scientific landscape of the phytoremediation of mine tailings through a series of bibliometric and scientometric techniques. Phytoremediation has emerged as a sustainable approach to remediate metal-contaminated mine waste areas. A scientometric analysis of 913 publications indexed in Web of Science from 1999 to 2023 was conducted using CiteSpace. The results reveal an expanding, interdisciplinary field with environmental sciences as the core category. Keyword analysis of 561 nodes and 2,825 links shows a focus on plant-metal interactions, microbial partnerships, bioavailability, and field validation. Co-citation analysis of 1,032 nodes and 2,944 links identifies seminal works on native species, plant-microbe interactions, and amendments. Temporal mapping of 15 co-citation clusters indicates a progression from early risk assessments and native plant inquiries to integrated biological systems, economic feasibility, and sustainability considerations. Recent trends emphasize multidimensional factors influencing adoption, such as plant-soil-microbe interactions, organic amendments, and field-scale performance evaluation. The findings demonstrate an intensifying translation of phytoremediation from scientific novelty to engineering practice. This quantitative and qualitative analysis of research trends aids in understanding the development of phytoremediation for mine tailings. The results provide valuable insights for researchers and practitioners in this evolving field.


Assuntos
Bibliometria , Biodegradação Ambiental , Mineração , Poluentes do Solo/metabolismo , Plantas/metabolismo
13.
Chemosphere ; 364: 142867, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39019183

RESUMO

Pesticides pose significant risks to both human health, such as cancer, neurological disorders, and endocrine disruption, and ecosystems, through the destruction of beneficial insects, contamination of soil and water, and impact on non-target species. In the face of escalating pesticide pollution, there is an urgent need for multifaceted approaches to address the issue. Bioremediation emerges as a potent tool in the environmental pollution mitigation arsenal. Ideally aiming for the complete decomposition of pesticides into harmless molecules, bioremediation encompasses diverse approaches - from bioabsorption, bioadsorption, and biotransformation using enzymes and nanoenzymes to comprehensive degradation facilitated by microorganisms such as bacteria, fungi, macro- and microalgae, or phytoremediation. Exploring nature's biodiversity offers a promising avenue to find solutions to this pressing human-induced problem. The acceleration of biodegradation necessitates identifying and developing efficient organisms, achieved through bioprospection and targeted modifications. Specific strategies to enhance process efficiency and throughput include optimizing biomass production, strategic inoculation in diverse environments, and employing bioreactor systems for processing heavily contaminated waters or soils. This comprehensive review presents various bioremediation approaches, emphasizing the importance of microorganisms' exploration and new technologies development, including current innovations and patents to effectively combat pesticide pollution. Furthermore, challenges regarding the effective implementation of these technologies are also addressed.


Assuntos
Biodegradação Ambiental , Praguicidas , Praguicidas/metabolismo , Bactérias/metabolismo , Poluição Ambiental/prevenção & controle , Humanos , Poluentes Ambientais/metabolismo , Fungos/metabolismo , Poluentes do Solo/metabolismo
14.
Sci Total Environ ; 949: 174876, 2024 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-39067601

RESUMO

Plastics derived from fossil fuels are used ubiquitously owing to their exceptional physicochemical characteristics. However, the extensive and short-term use of plastics has caused environmental challenges. The biotechnological plastic conversion can help address the challenges related to plastic pollution, offering sustainable alternatives that can operate using bioeconomic concepts and promote socioeconomic benefits. In this context, using soil from a plastic-contaminated landfill, two consortia were established (ConsPlastic-A and -B) displaying versatility in developing and consuming polyethylene or polyethylene terephthalate as the carbon source of nutrition. The ConsPlastic-A and -B metagenomic sequencing, taxonomic profiling, and the reconstruction of 79 draft bacterial genomes significantly expanded the knowledge of plastic-degrading microorganisms and enzymes, disclosing novel taxonomic groups associated with polymer degradation. The microbial consortium was utilized to obtain a novel Pseudomonas putida strain (BR4), presenting a striking metabolic arsenal for aromatic compound degradation and assimilation, confirmed by genomic analyses. The BR4 displays the inherent capacity to degrade polyethylene terephthalate (PET) and produce polyhydroxybutyrate (PHB) containing hydroxyvalerate (HV) units that contribute to enhanced copolymer properties, such as increased flexibility and resistance to breakage, compared with pure PHB. Therefore, BR4 is a promising strain for developing a bioconsolidated plastic depolymerization and upcycling process. Collectively, our study provides insights that may extend beyond the artificial ecosystems established during our experiments and supports future strategies for effectively decomposing and valorizing plastic waste. Furthermore, the functional genomic analysis described herein serves as a valuable guide for elucidating the genetic potential of microbial communities and microorganisms in plastic deconstruction and upcycling.


Assuntos
Biodegradação Ambiental , Microbiota , Plásticos , Plásticos/metabolismo , Microbiologia do Solo , Polietilenotereftalatos/metabolismo , Poluentes do Solo/metabolismo , Polímeros/metabolismo , Bactérias/metabolismo , Bactérias/genética , Plásticos Biodegradáveis/metabolismo , Consórcios Microbianos , Pseudomonas putida/metabolismo , Pseudomonas putida/genética
15.
World J Microbiol Biotechnol ; 40(8): 237, 2024 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-38853194

RESUMO

Industrial activities contribute to environmental pollution, particularly through unregulated effluent discharges, causing adverse effects on ecosystems. Vegetable oils, as insoluble substances, exacerbate this pollution, forming impermeable films and affecting the oxygen transfer, leading to serious habitat disruption. Organic wastes, such as soybean texturized waste, spent mushroom substrate, and stabilized poultry litter, were assessed for their efficacy in enhancing the degradation of vegetable oil in contaminated soil. For this purpose, contaminated soil was amended with each of the wastes (10% w/w) using microcosm systems, which were monitored physico-chemically, microbiologically and toxicologically. Results indicate that the wastes promoted significant oil degradation, achieving 83.1, 90.7, and 86.2% removal for soybean texturized waste, spent mushroom substrate, and stabilized poultry litter, respectively, within a 90-day period. Additionally, they positively influenced soil microbial activity, as evidenced by increased levels of culturable microorganisms and hydrolytic microbial activity. While bioassays indicated no phytotoxicity in most cases, soybean texturized waste exhibited inhibitory effects on seed germination and root elongation of Lactuca sativa. This study significantly enhances our comprehension of remediation techniques for sites tainted with vegetable oils, highlighting the critical role of organic waste as eco-friendly agents in soil restoration. Emphasizing the practical implications of these findings is imperative to underscore the relevance and urgency of addressing vegetable oil contamination in soil. Moving forward, tailored strategies considering both contaminant characteristics and soil ecosystem traits are vital for ensuring effective and sustainable soil remediation.


Assuntos
Biodegradação Ambiental , Glycine max , Óleos de Plantas , Aves Domésticas , Microbiologia do Solo , Poluentes do Solo , Solo , Animais , Poluentes do Solo/metabolismo , Glycine max/crescimento & desenvolvimento , Glycine max/microbiologia , Óleos de Plantas/metabolismo , Solo/química , Agaricales/metabolismo , Agaricales/crescimento & desenvolvimento , Lactuca/crescimento & desenvolvimento , Bactérias/metabolismo , Germinação/efeitos dos fármacos , Resíduos Industriais
16.
Braz J Microbiol ; 55(3): 2131-2147, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-38842788

RESUMO

Improper electronic waste management in the world especially in developing countries such as Iran has resulted in environmental pollution. Copper, nickel, and manganese are from the most concerned soil contaminating heavy metals which found in many electronic devices that are not properly processed. The aim of this study was to investigate the biological removal of copper, nickel, and manganese by Bacillus species isolated from a landfill of electronic waste (Zainal Pass hills located in Isfahan, Iran) which is the and to produce nanoparticles from the studied metals by the isolated bacteria. The amounts of copper, nickel, and manganese in the soil was measured as 1.9 × 104 mg/kg, 0.011 × 104 mg/kg and 0.013 × 104 mg/kg, respectively based on ICP-OES analysis, which was significantly higher than normal (0.02 mg/kg, 0.05 mg/kg, and 2 mg/kg, respectively. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of metals on the bacterial isolates was determined. The biosorption of metals by the bacteria was evaluated by inductively coupled plasma optical emission spectroscopy (ICP-OES). The metal nanoparticles were synthetized utilizing the isolates in culture media containing the heavy metals with the concentrations to which the isolates had shown resistance. X ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDS) were used for the evaluation of the fabrication of the produced metal nanoparticles. Based on the findings of this study, a total of 15 bacterial isolates were obtained from the soil samples. The obtained MICs of copper, nickel, and manganese on the isolates were 40-300 mM, 4-10 mM, and 60-120 mM, respectively. The most resistant isolates to copper were FM1 and FM2 which were able to bio-remove 79.81% and 68.69% of the metal, respectively. FM4 and FM5 were respectively the most resistant isolate to nickel and manganese and were able to bio-remove 86.74% and 91.96% of the metals, respectively. FM1, FM2, FM4, and FM5 was molecularly identified as Bacillus cereus, Bacillus thuringiensis, Bacillus paramycoides, and Bacillus wiedmannii, respectively. The results of XRD, SEM and EDS showed conversion of the copper and manganese into spherical and oval nanoparticles with the approximate sizes of 20-40 nm. Due to the fact that the novel strains in this study showed high resistance to copper, nickel, and manganese and high adsorption of the metals, they can be used in the future, as suitable strains for the bio-removal of these metals from electronic and other industrial wastes.


Assuntos
Bacillus , Cobre , Resíduo Eletrônico , Manganês , Nanopartículas Metálicas , Níquel , Microbiologia do Solo , Poluentes do Solo , Níquel/metabolismo , Níquel/química , Cobre/metabolismo , Bacillus/metabolismo , Bacillus/isolamento & purificação , Bacillus/classificação , Poluentes do Solo/metabolismo , Nanopartículas Metálicas/química , Manganês/metabolismo , Testes de Sensibilidade Microbiana , Irã (Geográfico) , Biodegradação Ambiental , Solo/química , Metais Pesados/metabolismo
17.
Environ Res ; 257: 119287, 2024 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-38823610

RESUMO

Heavy metal pollution in soil has emerged as a major environmental concern. This can be attributed to human activities such as mining, modern agriculture, and industrialization. This study was conducted to determine how heavy metals spread from mine tailings to surrounding farmland. Metal absorption and accumulation were also investigated in the root and shoot biomass of tapioca crops grown in those farmlands. Metal concentrations in MTAS1 were 85.3 ± 1.2, 45.8 ± 1.5, 134.8 ± 1.7, 92.4 ± 2.2, and 78.95 ± 1.4 mg kg-1, respectively. Heavy metal concentrations in MTAS2 and MTAS3 were found to be 79.62 ± 1.6, 75.4 ± 1.5, 41.31 ± 1.1, 47.8 ± 1.6, 142.5 ± 2.1, 128.4 ± 1.4, 86.2 ± 1.9, 79.5 ± 1.3, and 83.4 ± 1.2 mg kg-1, respectively. Tapioca crop shoot and root biomass grown at these metal polluted sites absorbed and accumulated significant amounts of Cd, Cu, Zn, Pb, Ni, and Mn. Notably, the metal content of the tapioca crop's root and shoot biomass exceeded national standards.


Assuntos
Biomassa , Metais Pesados , Mineração , Raízes de Plantas , Brotos de Planta , Poluentes do Solo , Metais Pesados/análise , Metais Pesados/metabolismo , Poluentes do Solo/análise , Poluentes do Solo/metabolismo , Raízes de Plantas/metabolismo , Raízes de Plantas/crescimento & desenvolvimento , Brotos de Planta/metabolismo , Brotos de Planta/crescimento & desenvolvimento , Manihot/crescimento & desenvolvimento , Manihot/metabolismo , Manihot/química , Agricultura/métodos , Monitoramento Ambiental
18.
Biodegradation ; 35(6): 939-949, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-38910213

RESUMO

The main challenge in treating aged soils highly contaminated with total petroleum hydrocarbons (TPH) is to enhance their bioavailability for microbial degradation. Hydrocarbons in soils undergo chemical changes that make them more resistant to biodegradation. This study investigates toluene's efficacy in enhancing the biodegradation of aged hydrocarbon-contaminated soil containing 292,000 mg TPH kg-1 dry soil. Toluene's effect was compared between solid phase (SOP) and slurry phase (SLP) treatments using a microbial consortium isolated from Cyperus laxus rhizosphere. TPH biodegradation and microbial respiration were measured, the latter to estimate the respiratory quotient (RQ, the ratio between moles of carbon dioxide released and moles of oxygen absorbed during respiration). Toluene significantly accelerated TPH biodegradation in both treatments, achieving ~ 30% higher removal than in a non-solvent control, possibly through improved bioavailability of aromatic compounds and other low molecular weight compounds. According to the RQ analysis, toluene enhanced microbial respiratory processes and hydrocarbon catabolism with higher hydrocarbon mineralization (RQ = ~ 0.5) in both SOP and SLP assays. Our results reveal toluene's potential to increase hydrocarbon availability and microbial degradation efficiency in aged contaminated soils; its use in various bioremediation techniques could be of broad applicability across diverse soil types and pollutants.


Assuntos
Biodegradação Ambiental , Hidrocarbonetos , Microbiologia do Solo , Poluentes do Solo , Tolueno , Tolueno/metabolismo , Poluentes do Solo/metabolismo , Hidrocarbonetos/metabolismo , Solo/química , Cyperus/metabolismo , Rizosfera , Petróleo/metabolismo
19.
Environ Pollut ; 357: 124431, 2024 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-38925214

RESUMO

Soil contamination by hydrocarbons is a problem that causes severe damage to the environment and public health. Technologies such as bioremediation using native microbial species represent a promising and environmentally friendly alternative for decontamination. This study aimed to isolate indigenous fungi species from the State of Rio de Janeiro, Brazil and evaluate their diesel degrading capacity in soils contaminated with crude oil. Seven filamentous fungi were isolated after enrichment cultivation from soils collected from contaminated sites and subjected to growth analysis on diesel nutrient media. Two fungal species were pre-selected and identified by morphological genus analysis and molecular techniques as Trichoderma asperellum and Penicillium pedernalense. The microdilution test showed that T. asperellum presented better fungal growth in high diesel concentrations than P. pedernalense. In addition, T. asperellum was able to degrade 41 and 54% of the total petroleum hydrocarbon (TPH) content present in soil artificially contaminated with diesel (10 g/kg of soil) in 7 and 14 days of incubation, respectively. In higher diesel concentration (1000 g of diesel/kg of soil) the TPH degradation reached 26%, 45%, and 48%, in 9, 16, and 30 d, respectively. The results demonstrated that the selected species was suitable for diesel degradation. We can also conclude that the isolation and selection process proposed in this work was successful and represents a simple alternative for obtaining native species with hydrocarbon degradation capacity, for use in the bioremediation process in the recovery of contaminated areas in an ecologically acceptable way.


Assuntos
Biodegradação Ambiental , Fungos , Gasolina , Hidrocarbonetos , Microbiologia do Solo , Poluentes do Solo , Poluentes do Solo/metabolismo , Brasil , Hidrocarbonetos/metabolismo , Fungos/metabolismo , Penicillium/metabolismo , Solo/química , Petróleo/metabolismo , Trichoderma/metabolismo
20.
Int J Phytoremediation ; 26(11): 1854-1862, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-38804225

RESUMO

The widespread use of petroleum-based fuels poses a significant environmental problem due to the risks associated with accidental spills. Among the treatments available, phytoremediation is increasingly accepted as an effective and low-cost solution. This study aimed to evaluate the degradation of the aromatic fraction of biodiesel B10 and the soil fertility analysis in artificially contaminated soils treated with phytoremediation. The experimental design consisted of a 3x3 factorial, with three types of soil treatment: control, autoclaved, and planted with C. ensiformis L, and three levels of B10 biodiesel contamination: 0, 1, and 2%, to simulate spills of 30,000 and 60,000 L ha-1. The soil was analyzed at three depths: 0-10, 10-20, and 20-30 cm. The results indicated that aromatic compound degradation after phytoremediation was superior to 92,76% and 88,65% for 1% and 2% B10 soil contamination, respectively. The fuel contamination affected soil fertility, reducing the availability of phosphorus and zinc while increasing the Total Organic Carbon (TOC), pH, and the availability of manganese and iron for plants.


Numerous studies have been carried out on the phytoremediation of soils contaminated with diesel. However, the potential impact of biodiesel-diesel blends on soil fertility has not been fully explored, leading to a critical knowledge gap. This work investigates the novel effects of biodiesel-diesel blends on phytoremediation and soil fertility, allowing us to expand our understanding of the effectiveness of this approach to remediate contaminated sites.


Assuntos
Biodegradação Ambiental , Biocombustíveis , Poluentes do Solo , Solo , Poluentes do Solo/metabolismo , Solo/química , Traqueófitas/metabolismo
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