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1.
Medicine (Baltimore) ; 99(1): e18635, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31895824

RESUMO

This cross sectional study examined patients' perceptions of professional support regarding use of psychotropic medication in a specialist mental health care setting. The aims were to evaluate reliability and validity of the MedSupport inventory, and investigate possible associations between MedSupport scores and patient characteristics.A cross-sectional study was performed. The patients completed the MedSupport, a newly developed self-reported 6 item questionnaire on a Likert scale ranged 1 to 5 (1 = strongly disagree to 5 = strongly agree), and the Beliefs about Medicines Questionnaire. Diagnosis and treatment information were obtained at the clinical visits and from patient records.Among the 992 patients recruited, 567 patients (57%) used psychotropic medications, and 514 (91%) of these completed the MedSupport and were included in the study. The MedSupport showed an adequate internal consistency (Cronbach alpha.87; 95% CI.86-89) and a convergent validity toward the available variables. The MedSupport mean score was 3.8 (standard deviation.9, median 3.8). Increasing age and the experience of stronger needs for psychotropic medication were associated with perception of more support to cope with medication, whereas higher concern toward use of psychotropic medication was associated with perception of less support. Patients diagnosed with behavioral and emotional disorders, onset in childhood and adolescence perceived more support than patients with Mood disorders.The MedSupport inventory was suitable for assessing the patients' perceived support from health care service regarding their medication. Awareness of differences in patients' perceptions might enable the service to provide special measures for patients who perceive insufficient medication support.


Assuntos
Transtornos Mentais/tratamento farmacológico , Serviços de Saúde Mental , Psicotrópicos/uso terapêutico , Adulto , Estudos Transversais , Feminino , Humanos , Masculino , Transtornos Mentais/psicologia , Pessoa de Meia-Idade , Reprodutibilidade dos Testes , Inquéritos e Questionários , Adulto Jovem
2.
Medicine (Baltimore) ; 99(1): e18569, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31895800

RESUMO

An adverse drug reactions avoidability tool called the Liverpool ADR avoidability assessment tool (LAAT) was recently developed (for research purposes), and subsequently validated with mixed interrater reliability (IRR). We investigated the comparative IRR of this tool in an inpatient cohort to ascertain its practical application in this setting.The patient population was comprised of 44 ADR drug pairs drawn from an observational prospective cohort of patents with ADR attending a Weill Cornell Medicine-affiliated tertiary medical Centre in Doha Qatar (Hamad General Hospital). Using the LAAT, and modified Hallas tools, 4 independent raters (2 Clinical Pharmacologists, and 2 General Physicians) assessed and scored the 44 ADR-drug pairs. Agreement proportions between the rating pairs were evaluated as well individual/overall kappa statistics and intraclass correlation coefficients. We evaluated the weight of each of the 7 questions on the LAAT tool to ascertain its determinative role.Across 44 ADR-drug pairs, the overall median Fleiss kappa using the LAAT, and modified Hallas tools were 0.67 (interquartile range (IQR) 0.55, 0.76), 0.36 (IQR, 0.23-0.71) respectively. The overall percentage pairwise agreement with the LAAT and modified Hallas tools were 78.5%, and 62.2% respectively. Exact pairwise agreement occurred in 37 out of 44 (range 0.71-1), and 27 of 44 (0.53-0.77) ADR-drug pairs using the LAAT and modified Hallas tools respectively. Using the LAAT tool, the overall intraclass correlation coefficient was 0.68 (CI 0.55, 0.79), and 0.37 (CI 0.22, 0.53) with the modified Hallas tool.We report a higher proportion of "possible" and "definite" avoidability outcomes of adverse drug reactions compared with the modified Hallas, or that reported by developers of the LAAT tool. Although initially developed for research purposes, our report has suggested for the first time a potential applicability of this tool in clinical environment as well.


Assuntos
Rotas de Resultados Adversos , Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos/prevenção & controle , Adulto , Algoritmos , Feminino , Humanos , Pacientes Internados/estatística & dados numéricos , Masculino , Pessoa de Meia-Idade , Variações Dependentes do Observador , Estudos Prospectivos , Catar , Reprodutibilidade dos Testes
3.
Food Chem ; 307: 125645, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31644983

RESUMO

A simple electrochemical sensor was developed to determine the concentration of Ca2+ in meat. Graphene was treated with oxygen plasma for 10 s and 30 s comparing with the pristine graphene. Through analyzing morphology and chemical composition, the graphene with the lowest defect density was chosen to mix with bovine serum albumin molecule-functionalized gold nanoparticles. It was interesting that only a few gold nanoparticles were trapped in the graphene with 10 s plasma treatment. Then, under the optimal condition measured, the limit of detection was detected as 3.9 × 10-8 M with a linear relationship from 5 × 10-8 to 3 × 10-4 M. Finally, the proposed electrochemical method was applied to detect Ca2+ in the pork sample with stability and reproducibility verified by parallel detections. Thus, the proposed method demonstrates its potential for effectively detecting Ca2+ in meat and prominently reduces time consumption on operations and pretreatments.


Assuntos
Técnicas Biossensoriais/instrumentação , Cálcio na Dieta/análise , Técnicas Eletroquímicas/métodos , Carne/análise , Nanopartículas Metálicas/química , Técnicas Biossensoriais/métodos , Cálcio na Dieta/metabolismo , Ouro/química , Grafite/química , Limite de Detecção , Reprodutibilidade dos Testes , Troponina C/metabolismo
4.
Food Chem ; 305: 125426, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31522124

RESUMO

Genetically modified (GM) Atlantic salmon, AquAdvantage (AquAd), was the first GM animal approved officially for human consumption. Many countries monitor the use of this product under their GM regulations, but a pragmatic system for AquAd-specific detection is needed. Here, we developed a real-time polymerase chain reaction method with high sensitivity for detection of AquAd in foods. This method showed high specificity for the AquAd transgene and the detection limit was 12.5-25 targeted DNA copies per test reaction. An inter-laboratory study using the method developed demonstrated reproducibility at >0.1% (w/w) AquAd content.


Assuntos
Alimentos Geneticamente Modificados , Reação em Cadeia da Polimerase em Tempo Real/métodos , Salmo salar/genética , Alimentos Marinhos/análise , Animais , Animais Geneticamente Modificados , Reprodutibilidade dos Testes
5.
Spec Care Dentist ; 40(1): 71-78, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31858622

RESUMO

AIMS: The dental activities test (DAT), designed to assess individuals' ability to perform oral health-related activities, was initially tested among 90 assisted living residents with normal to severely impaired cognition. This study aimed to examine the reliability (internal consistency), convergent validity, and structural validity of DAT among individuals with stroke. METHODS AND RESULTS: This study was a secondary analysis of data collected for a cross-sectional study that aimed to understand oral disease patterns among 207 individuals with stroke from three hospitals in China. Convergent validity was examined using Spearman's Correlation coefficient (rs ) for correlation between DAT scores and the assessments of physical function and cognition. Structural validity was examined using Confirmatory Factor Analysis (CFA). The DAT demonstrated good estimates for internal consistency (Kuder Richardson-20 = 0.85, 95% confidence of interval [CI] = 0.82, 0.88), convergent validity (rs range: 0.43-0.61, all P < .001), and good structural validity with a one-factor structure. CONCLUSION: Findings supported the reliability and validity of DAT as a unidimensional construct in measuring the ability to perform oral health-related activities in persons with stroke. Further testing among different patient populations and care settings is needed to accumulate evidence and expand the use of the test.


Assuntos
Acidente Vascular Cerebral , China , Estudos Transversais , Análise Fatorial , Humanos , Psicometria , Reprodutibilidade dos Testes , Inquéritos e Questionários
6.
Chemosphere ; 239: 124666, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31479911

RESUMO

The toxicity of polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs) is well known, and for this reason studying and monitoring these chemicals is fundamental. Activated carbon fibers (ACFs) are made of an adsorbent material widely used in the industrial field for the removal of micropollutants. The first step in this work was to perform a physico-chemical characterization of the adsorbent, focused on the analytical use of it. In particular, its specific surface area was defined around 2500 m2/g consisting in a homogeneous microporosity distribution and the characterization of ACF surface functional groups pointed out a balance between basic and acidic group. The validity of using the ACF as solid phase extraction and as passive sampler for PCDD/Fs and PCBs in water, has been evaluated by the percentage recovery (R %) of 13C12-labeled standards of PCDD/Fs and PCBs added in a known volume of water. The results were compared to the R% of Liquid-Liquid Extraction which showed a better reproducibility of the results and the proposed method satisfy completely the requirements of US EPA reference methods.


Assuntos
Carvão Vegetal/química , Dibenzofuranos Policlorados/análise , Monitoramento Ambiental/métodos , Recuperação e Remediação Ambiental/métodos , Bifenilos Policlorados/análise , Dibenzodioxinas Policloradas/análise , Benzofuranos/análise , Fibra de Carbono , Reprodutibilidade dos Testes , Extração em Fase Sólida , Água
7.
Chemosphere ; 239: 124670, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31505441

RESUMO

The objectives of this study were to determine the viability of removing Orange II (OII) dye by simulated solar photoelectro-Fenton (SSPEF) and to evaluate the stability of a WO2.72/Vulcan XC72 gas diffusion electrode (GDE) and thus determine its best operating parameters. The GDE cathode was combined with a BDD anode for decolorization and mineralization of 350 mL of 0.26 mM OII by anodic oxidation with electrogenerated H2O2 (AO-H2O2), electro-Fenton (EF) and photoelectro-Fenton (PEF) at 100, 150 and 200 mA cm-2 and SSPEF at 150 mA cm-2. The GDE showed successful operation for electrogeneration, good reproducibility and low leaching of W. Decolorization and OII decay were directly proportional to the current density (j). AO-H2O2 had a reduced performance that was only half of the SSPEF, PEF and EF treatments. The mineralization efficiency was in the following order: AO-H2O2 < EF < PEF ≈ SSPEF. This showed that the GDE, BDD anode and light radiation combination was advantageous and indicated that the SSPEF process is promising with both a lower cost than using UV lamps and simulating solar photoelectro-Fenton process. The PEF process with the lowest j (100 mA cm-2) showed the best performance-mineralization current efficiency.


Assuntos
Compostos Azo/análise , Benzenossulfonatos/análise , Técnicas Eletroquímicas , Peróxido de Hidrogênio/química , Ferro/química , Poluentes Químicos da Água/análise , Eletrodos , Oxirredução , Reprodutibilidade dos Testes , Luz Solar
8.
Chemosphere ; 240: 124953, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31574435

RESUMO

Chemical characterization of PM2.5 and PM10 is important to identify potential compounds that induce biological responses that translate into cardio-respiratory health problems. This study shows the reliability of the use of crystalline phases, identified in samples from receptor sites, as source markers, helping researchers to infer the main sources of air pollution, even without the use of receptor models. PM2.5 and PM10 samples were collected at two sites in an urban industrialized region located at southeast of Brazil and analyzed by Synchrotron X-ray Diffraction to identify crystalline compounds. Results show 5 PM10 and PM2.5 species not previously reported in the literature. We propose reaction mechanisms for these species and identify specific sources for each crystalline phase found: BaTiO3 was found in PM10 receptor samples and proved to be a vehicular marker formed during brake action; maghemite (γ-Fe2O3), pyracmonite [(NH4)3Fe(SO4)3], ammonium perchlorate (NH3OHClO4) and potassium ferrate (K2Fe2O4) were found in PM2.5 proved to be markers of industrial activities. The crystalline phases found in PM samples from receptor sites and the mechanisms of reactions showed the reliability of the use of crystalline phases as source markers in the identification of potential sources of air pollution without misinterpretation of the likely source.


Assuntos
Poluentes Atmosféricos/análise , Poluição do Ar/análise , Monitoramento Ambiental/métodos , Material Particulado/análise , Material Particulado/química , Sulfato de Amônio/análise , Compostos de Bário/análise , Brasil , Compostos Férricos/análise , Indústrias , Compostos de Ferro/análise , Tamanho da Partícula , Percloratos/análise , Compostos de Potássio/análise , Compostos de Amônio Quaternário/análise , Reprodutibilidade dos Testes , Síncrotrons , Titânio/análise , Difração de Raios X/métodos
9.
Ann Otol Rhinol Laryngol ; 129(1): 12-17, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31394908

RESUMO

OBJECTIVE: To define a new anatomic relationship in pediatric sinus surgery, assessing the maxillary roof as a constant safe landmark to avoid skull base injury in the pediatric population. STUDY DESIGN: Retrospective analysis. SETTING: Tertiary care children hospital. SUBJECTS AND METHODS: A retrospective analysis was performed of all computed tomography scans of the sinuses and facial bones at the emergency department of a tertiary children's hospital over the course of a year. Radiographic measurements included the lowest cribriform plate and planum sphenoidale (PS) heights, or posterior skull base when not yet pneumatized, as well as the highest maxillary roof height. The nasal floor was used for reference. Statistics were performed via Shapiro-Wilks test with a P-value of .05 indicating statistical significance. RESULTS: Three hundred and seven unique scans were reviewed (38.9% female; n = 122; P = .58). Age stratification was based on previously described sinus growth patterns. In all patients, the maximum maxillary height was inferior to the lowest measured cribriform lamella and PS (P < .001; CI, 98.5%-99%). Inter- and intrarater reliability and accuracy were verified through blinded review and re-review (ρ = .99 and .98 respectively, P ≤ .001). The validity of sole coronal measurements due to incomplete sagittal reformatting was also confirmed (ρ = 1.00, P ≤ .001). CONCLUSION: Despite variation in sinus growth and development in children, the current study demonstrated the validity of the maxillary sinus roof as a constant safe landmark in the pediatric population, offering a novel anatomic relationship for teaching safety in performing pediatric sinus surgery. LEVEL OF EVIDENCE: 4.


Assuntos
Pontos de Referência Anatômicos , Endoscopia/métodos , Seio Maxilar/diagnóstico por imagem , Procedimentos Cirúrgicos Otorrinolaringológicos/métodos , Base do Crânio/diagnóstico por imagem , Adolescente , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Seio Maxilar/cirurgia , Reprodutibilidade dos Testes , Estudos Retrospectivos , Base do Crânio/cirurgia , Tomografia Computadorizada por Raios X , Adulto Jovem
11.
Food Chem ; 305: 125512, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31610422

RESUMO

This study represents the first attempt to combine mid infrared (MIR) spectroscopy and multivariate data processing for prediction of alcohol degree, sugars content and total acidity in straw wine. 302 Italian samples, representing different vintages, production regions and grape varieties, were analysed using FT-MIR spectroscopy and reference methods. New regression functions based on a combination of Orthogonal Signal Correction and Partial Least Squares regression are proposed for prediction of quality parameters: this approach allows overcoming the issue of matrix complexity, reducing spectral interferences and enhancing the information embodied in fingerprinting data. The models proposed are characterised by an excellent reliability, with low error in prediction (alcohol: 0.28%; sugars: 9.9 g/L; acidity: 0.29 g/L) comparable both to reference methods and table wine models. Results demonstrate that vibrational spectroscopy, combined with a proper multivariate data strategy, represents a suitable strategy for the quick and non-destructive assessment of quality parameters of straw wine.


Assuntos
Qualidade dos Alimentos , Informática/métodos , Espectroscopia de Infravermelho com Transformada de Fourier , Vinho/análise , Análise dos Mínimos Quadrados , Análise Multivariada , Reprodutibilidade dos Testes , Vitis/química
12.
J Environ Manage ; 254: 109825, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31733467

RESUMO

Microalgae harvesting is one of the major bottlenecks for the production of high-value microalgal products on a large scale, which encourages investigations of harvesting methods with better cost-benefits. Among these harvesting techniques, flotation stands out as a promising method, however it is still minimally explored when compared to the sedimentation method. In this study, the pH modulation followed by dissolved air flotation (DAF) was tested as a harvesting method for Chlorella sorokiniana cultivated in wastewater. The main aims of this study were to optimize the operational parameters of coagulation (pH, velocity gradient, and mixing time) and flotation (recirculation rate), check their reproducibility and resilience with the variability of wastewater characteristics, and evaluate the final wastewater quality after treatment using an optimized harvesting method. Parameter optimization was carried out using the one-factor-at-a-time method. The optimal parameters were a velocity gradient of 500 s-1, mixing time of 30 s, pH 12, and 20% of recirculation rate. High efficiencies were obtained for C. sorokiniana removal (96.5-97.9%), making it a successful process. Moreover, the photobioreactor effluent quality was also improved significantly after microalgae harvesting, with high nutrient removal (88.6-95.1% of total Kjeldahl nitrogen and 91.8-98.3% of total phosphorus) and organic matter removal (80.5-86.8% of chemical oxygen demand). The results showed the pH modulation and DAF as an effective process for wastewater treatment and biomass harvesting. This study also indicated the importance of operational optimization, not studied until now, in which the achieved results could be potentially applied as practical guidelines for microalgae harvesting on a large scale.


Assuntos
Chlorella , Microalgas , Biomassa , Concentração de Íons de Hidrogênio , Reprodutibilidade dos Testes , Águas Residuárias
13.
Angiology ; 71(1): 38-47, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31554413

RESUMO

This study investigated whether a novel index of stress hyperglycemia might have a better prognostic value compared to admission glycemia alone in patients with ST-segment elevation myocardial infarction (STEMI) undergoing percutaneous coronary intervention (PCI). The acute-to-chronic glycemic ratio was expressed as admission blood glucose (ABG) devided by the estimated average glucose (eAG), and eAG was derived from the glycated hemoglobin (HbA1c). A total of 1300 consecutive patients with STEMI treated with PCI were included. Baseline data and outcomes were analyzed. The study end point was a composite of in-hospital all-cause death, cardiogenic shock, and acute pulmonary edema. Accuracy was defined with area under the curve (AUC) by a receiver-operating characteristic (ROC) curve analysis. After multivariate adjustment, both ABG/eAG and ABG were closely associated with an increased risk of the composite end point in nondiabetic patients. However, only ABG/eAG (odds ratio = 2.45, 95% confidence interval: 1.24-4.82, P = .010), instead of ABG, was associated with the outcomes in diabetic patients. Compared to ABG, ABG/eAG had an equivalent predictive value in nondiabetic patients but a superior discriminatory ability in diabetic patients (AUC improved from 0.52-0.63, P < .001). Taken together, ABG/eAG provides more significant in-hospital prognostic information than ABG in diabetic patients with STEMI after PCI.


Assuntos
Glicemia/metabolismo , Hemoglobina A Glicada/metabolismo , Admissão do Paciente , Intervenção Coronária Percutânea , Infarto do Miocárdio com Supradesnível do Segmento ST/terapia , Idoso , Biomarcadores/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Intervenção Coronária Percutânea/efeitos adversos , Valor Preditivo dos Testes , Reprodutibilidade dos Testes , Infarto do Miocárdio com Supradesnível do Segmento ST/sangue , Infarto do Miocárdio com Supradesnível do Segmento ST/diagnóstico , Fatores de Tempo , Resultado do Tratamento
14.
Sports Biomech ; 19(1): 1-25, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29781788

RESUMO

The use of isometric strength testing, particularly the isometric mid-thigh pull (IMTP) has increased dramatically over the last decade. The IMTP and isometric squat (ISqT) provide one aspect of performance monitoring with variables such as peak force and rate of force development being derived from the force-time curve. The reliability of some of these variables is conflicting in the literature, and the reporting of the reliability is not standardised across the research. The majority of research only reports intraclass correlation coefficients with very few studies reporting coefficient of variation and 90% confidence intervals. Additionally, methods used to calculate variables from the force-time curve differ across studies. An aim of muscle strength testing is to provide normative values for specific sports, allowing coaches to distinguish between performance levels or evaluate the effects of training on performance. This narrative review aims to evaluate studies that have researched the reliability and/or reported normative data for both tests. Additionally, the testing protocols and the force-time curve analysis techniques utilised are discussed, concluding with practical applications for coaches on the uses and limitations of these tests. Results demonstrate that peak force is the most reliable measure and can be used to determine maximum strength capabilities.


Assuntos
Teste de Esforço/métodos , Força Muscular/fisiologia , Coxa da Perna/fisiologia , Levantamento de Peso , Fenômenos Biomecânicos , Humanos , Contração Isométrica/fisiologia , Valores de Referência , Reprodutibilidade dos Testes , Esportes/fisiologia
15.
Bioelectrochemistry ; 131: 107350, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31518962

RESUMO

Curcumin (Cur), the yellow pigment of well-known turmeric (Curcuma longa L.) is effective in multiple cancers including triple negative breast cancer (TNBC). In combination with electrical pulses (EP), enhanced effects of curcumin (Cur + EP) are observed in TNBC cells. To gain insights into the mechanisms of enhanced anticancer effects of Cur + EP, we studied the proteins involved in the anticancer activity of Cur + EP in MDA-MB-231, human TNBC cells using high-throughput global proteomics. A curcumin dose of 50 µM was applied with eight, 1200 V/cm, 100 µs pulses, the most commonly used electrochemotherapy (ECT) parameter in clinics. Results show that the Cur + EP treatment reduced the clonogenic ability in MDA-MB-231 cells, with the induction of apoptosis. Proteomic analysis identified a total of 1456 proteins, of which 453 proteins were differentially regulated, including kinases, heat shock proteins, transcription factors, structural proteins, and metabolic enzymes. Eight key glycolysis proteins (ALDOA, ENO2, LDHA, LDHB, PFKP, PGM1, PGAM1 and PGK1) were downregulated in Cur + EP from Cur. There was a switch in the metabolism with upregulation of 10 oxidative phosphorylation pathway proteins and 8 tricarboxylic acid (TCA) cycle proteins in the Cur + EP sample, compared to curcumin. These results provide novel systematic insights into the mechanisms of ECT with curcumin.


Assuntos
Antineoplásicos/uso terapêutico , Curcumina/uso terapêutico , Eletroquimioterapia/métodos , Proteínas de Neoplasias/metabolismo , Proteômica , Neoplasias de Mama Triplo Negativas/tratamento farmacológico , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Curcumina/farmacologia , Regulação para Baixo/efeitos dos fármacos , Feminino , Glicólise , Humanos , Fosforilação Oxidativa , Via de Pentose Fosfato/efeitos dos fármacos , Reprodutibilidade dos Testes , Neoplasias de Mama Triplo Negativas/metabolismo , Neoplasias de Mama Triplo Negativas/patologia
16.
Bioelectrochemistry ; 131: 107393, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31698180

RESUMO

A tryptophan (Trp) molecularly imprinted electrochemical sensor was fabricated by drop-coating an imprinted chitosan film on the surface of a glassy carbon electrode modified with multi-walled carbon nanotubes (MIP-MWCNTs/GCE). The surface morphology and electrochemical properties of the MIP-MWCNTs/GCE were characterized by scanning electron microscopy (SEM) and cyclic voltammetry (CV), respectively. The formation of hydrogen bonds between the functional polymer and the template molecule was confirmed by infrared spectroscopy. The electrochemical performance of the MIP-MWCNTs/GCE with Trp showed that the signal of the oxidation current of Trp obtained with MIP-MWCNTs/GCE was significantly enhanced relative to that of the uncovered GCE, indicating that the modified electrode can accelerate electron transfer and has strong selectivity for Trp. The experimental conditions were optimized in parallel, and under the optimal conditions, the MIP-MWCNTs/GCE showed a good linear relationship between the Trp oxidation peak current and Trp concentrations in the ranges of 2.0 nM-0.2 µM, 0.2 µM-10 µM and 10 µM-100 µM The limit of detection (LOD) was 1.0 nM (S/N = 3), and the modified electrode had good reproducibility and stability. Finally, the MIP-MWCNTs/GCE was successfully applied to the determination of Trp in the human serum samples.


Assuntos
Carbono/química , Eletrodos , Impressão Molecular , Nanotubos de Carbono/química , Triptofano/análise , Técnicas Eletroquímicas/métodos , Reprodutibilidade dos Testes , Espectroscopia de Infravermelho com Transformada de Fourier
17.
Food Chem ; 308: 125647, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-31648088

RESUMO

An analyser was constructed on the basis of on-line connection of capillary electrophoresis over a short separation path with continuous mini-dialysis sample collection. The developed instrument was employed for simultaneous determination of the majority minerals K+, Ca2+, Na+ and Mg2+ (and possibly NH4+ ions) in commercially available unflavoured yoghurts. The cations are released from the organic structures by digestion with boiling 6 mol/L HCl. They were separated from residues of the organic matrix by a dialysis probe and were transferred to a stream of water. From the continuous stream, the dialysate was injected into the separation capillary through a flow-gating interface. Within the reliability interval, the determined total mineral content was equal to their contents stated on the yoghurt labels and the content determined by flame atomic absorption spectrometry and complexometric titration. The relative standard deviation of the electrophoretic determination is mostly about 5%.


Assuntos
Iogurte/análise , Cátions/química , Diálise , Eletroforese Capilar/métodos , Reprodutibilidade dos Testes , Espectrofotometria Atômica , Fatores de Tempo , Água/análise
18.
Dentomaxillofac Radiol ; 49(1): 20190107, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31386555

RESUMO

OBJECTIVES: To investigate the current clinical applications and diagnostic performance of artificial intelligence (AI) in dental and maxillofacial radiology (DMFR). METHODS: Studies using applications related to DMFR to develop or implement AI models were sought by searching five electronic databases and four selected core journals in the field of DMFR. The customized assessment criteria based on QUADAS-2 were adapted for quality analysis of the studies included. RESULTS: The initial electronic search yielded 1862 titles, and 50 studies were eventually included. Most studies focused on AI applications for an automated localization of cephalometric landmarks, diagnosis of osteoporosis, classification/segmentation of maxillofacial cysts and/or tumors, and identification of periodontitis/periapical disease. The performance of AI models varies among different algorithms. CONCLUSION: The AI models proposed in the studies included exhibited wide clinical applications in DMFR. Nevertheless, it is still necessary to further verify the reliability and applicability of the AI models prior to transferring these models into clinical practice.


Assuntos
Inteligência Artificial , Radiografia Dentária , Radiologia , Algoritmos , Inteligência Artificial/normas , Inteligência Artificial/tendências , Humanos , Radiografia Dentária/métodos , Radiografia Dentária/tendências , Reprodutibilidade dos Testes
19.
J Clin Pathol ; 73(1): 35-41, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31296605

RESUMO

AIMS: Precision medicine therapy is remodelling the diagnostic landscape of cancer. The success of these new therapies is often based on the presence or absence of a specific mutation in a tumour. The Idylla platform is designed to determine the mutational status of a tumour as quickly and accurately as possible, as a rapid, accurate diagnosis is of the utmost importance for the treatment of patients. This is the first complete prospective study to investigate the robustness of the Idylla platform for EGFR, KRAS and BRAF mutations in non-small cell lung cancer, metastatic colorectal cancer and metastatic melanoma, respectively. METHODS: We compared prospectively the Idylla platform with the results we obtained from parallel high-throughput next-generation sequencing, which is the current gold standard for mutational testing. Furthermore, we evaluated the benefits and disadvantages of the Idylla platform in clinical practice. Additionally, we reviewed all the published Idylla performance articles. RESULTS: There was an overall agreement of 100%, 94% and 94% between the next-generation panel and the Idylla BRAF, KRAS and EGFR mutation test. Two interesting discordant findings among 48 cases were observed and will be discussed together with the advantages and shortcoming of both techniques. CONCLUSION: Our observations demonstrate that the Idylla cartridge for the EGFR, KRAS and BRAF mutations is highly accurate, rapid and has a limited hands-on time compared with next-generation sequencing.


Assuntos
Biomarcadores Tumorais/genética , Análise Mutacional de DNA/métodos , Sequenciamento de Nucleotídeos em Larga Escala , Mutação , Neoplasias/genética , Proteínas Proto-Oncogênicas B-raf/genética , Proteínas Proto-Oncogênicas p21(ras)/genética , Carcinoma Pulmonar de Células não Pequenas/genética , Carcinoma Pulmonar de Células não Pequenas/patologia , Neoplasias Colorretais/genética , Neoplasias Colorretais/patologia , Receptores ErbB/genética , Humanos , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/patologia , Melanoma/genética , Melanoma/secundário , Neoplasias/patologia , Valor Preditivo dos Testes , Estudos Prospectivos , Reprodutibilidade dos Testes , Neoplasias Cutâneas/genética , Neoplasias Cutâneas/patologia , Fluxo de Trabalho
20.
J Clin Pathol ; 73(1): 1-6, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31308255

RESUMO

Traditionally, immunohistochemistry (IHC) is used by pathologists to localise specific proteins or peptides in tissue slides. In the era of personalised medicine, however, molecular tissue analysis becomes indispensable for correct diagnosis, prognosis and therapeutic decision, not only on the DNA or mRNA level but also on the protein level. Combining molecular information with imaging presents many advantages. Therefore, matrix-assisted laser desorption/ionisation imaging mass spectrometry (MALDI IMS) is a promising technique to be added to the armamentarium of the pathologist. Here, we focus on the workflow, advantages and drawbacks of both MALDI IMS and IHC. We also briefly discuss a few other protein imaging modalities and give examples of applications.


Assuntos
Ensaios de Triagem em Larga Escala , Imuno-Histoquímica , Serviço Hospitalar de Patologia , Patologia Clínica/métodos , Proteômica/métodos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Difusão de Inovações , Humanos , Valor Preditivo dos Testes , Reprodutibilidade dos Testes , Fluxo de Trabalho
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