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1.
Food Chem ; 368: 130777, 2022 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-34392118

RESUMO

In this study, oils extracted from nigella seeds (NS) subjected to microwave roasting at 180, 360, 540 and 720 W for 5 and 10 min were evaluated for quality and stability characteristics. The impact of microwave powers on oil yield, pigment content, Maillard reaction products (MRPs), radical scavenging activity (RSA), color, viscosity, total phenolic content (TPC), oxidative stability index (OSI) and fatty acid composition (FAC) of oil was studied. TPC, RSA, OSI, MRPs, viscosity, chlorophyll and carotenoid contents were higher in oil of NS heated at 720 W for 10 min while maximum oil yield and low acid value was observed for NS heated at 540 W for 10 min. FAC was slightly influenced by microwave roasting and FTIR spectra exhibited a minor difference in intensities of peaks at 3009, 2925, 2854, 1745 and 1161 cm-1. The results of the study indicated that microwave roasting improves NS oil quality characteristics.


Assuntos
Nigella sativa , Nigella , Antioxidantes , Produtos Finais de Glicação Avançada , Micro-Ondas , Estresse Oxidativo , Óleos Vegetais , Sementes
2.
Food Chem ; 368: 130842, 2022 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-34419794

RESUMO

This study proposes a preliminary assessment of the homogeneity and stability through digital image acquisition of a candidate for mechanically processed pumpkin seed meal reference material, exploring the concepts of homogeneity curve and the analysis of texture characteristics by Continuous-Level Moving Block through Robust Principal Component Analysis. This innovative methodology allowed us to examine the percentage of homogeneity in a set of samples, revealing an average of 41% with only one outlier in relation to the entire sample, indicating low homogeneity. In the stability study carried out after storing samples for 12 months at different temperatures, 83% of the samples were considered regular and 17% were outlier, which means that most of them were considered stable. Therefore, this methodology is useful for screening samples for homogeneity, by textural analysis, and detected non-homogeneity can be corrected in advance for quantification by standard protocols.


Assuntos
Cucurbita , Farinha , Computadores , Farinha/análise , Análise de Componente Principal , Padrões de Referência , Sementes
3.
Food Chem ; 368: 130799, 2022 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-34425343

RESUMO

This study aims to investigate the changes in physical and chemical properties of edamame during bean development and apply a spectroscopy-based machine learning (ML) technique to determine optimal harvest time. The edamame harvested at R5 (beginning seed), R6 (full seed), and R7 (beginning maturity) growth stages were characterized for physical and chemical properties, and pods were measured for spectral reflectance (360-740 nm) using a handheld spectrophotometer. The samples were categorized into 'early', 'ready', and 'late' based on the characterized properties. The results showed that pod/bean weight and pod thickness peaked at R6 and remained stable thereafter. Sugar, starch, alanine, and glycine also peaked at R6 but proceeded to decline. The ML method (random forest classification) using pods' spectral reflectance had a high accuracy of 0.95 for classifying 'early' and 'late' samples and 0.87 for classifying 'early' and 'ready' samples. Therefore, this method can determine the optimal harvest time of edamame.


Assuntos
Aprendizado de Máquina , Soja , Sementes , Análise Espectral , Açúcares
4.
Food Chem ; 366: 130645, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34325243

RESUMO

The chemical and thermal characteristics of goldenberry pomace oil (GPO) and goldenberry seed oil (GSO) were investigated. GPO and GSO contained high levels of unsaturated fatty acids (90.1% and 85.1%, respectively), and the major fatty acid was linoleic (62.0% and 72.8%, respectively). Additionally, GPO contained eleven triacylglycerol (TAG) species, three of which represented 82.7%, namely C54:6, C54:4 and C52:4, and trilinolein was the dominant one (35.5%). GSO contained nine TAG species, two of which represented 80.3%, namely C54:6 and C52:4, and trilinolein was dominant (53.3%). The DSC analysis of GPO and GSO revealed that three exothermal peaks were detected during cooling. Three endothermal peaks (one of which is exothermal for GSO) were detected during melting, and the most significant peaks occurred at low temperatures. FTIR spectra indicated that GPO and GSO did not contain peroxides or trans fatty acids, but they did contain low concentrations of free fatty acids.


Assuntos
Physalis , Cristalização , Ácidos Graxos , Sementes , Espectroscopia de Infravermelho com Transformada de Fourier
5.
Food Chem ; 367: 130647, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34343806

RESUMO

To increase the reuse of food residues, multifrequency countercurrent ultrasonic treatment was used to enhance the extraction yield of defatted mulberry seed protein (DMSP), and sweet-flavored peptides from DMSP hydrolysates (DMSPHs) were obtained for the first time. Here, the DMSP yield was increased by 16.2% (p < 0.05) while the power density was halved compared with single-frequency ultrasonic treatment. According to Fick's second law, a molecular diffusion dynamics model was developed to be suitable for predicting the pretreatment conditions (R2 = 0.9785). After that, the sweet-flavored peptides were purified and the main amino acid sequences were identified, i.e., FEGGSIE, KDFPEAHSQAT, and GSQPAEGAK. Moreover, the antioxidant activities of DMSPHs prepared with tri-frequency treatment was higher than 60%. The DMSPHs retarded the growth of HepG2 cells in vitro, increased the necrotic quadrant (Q1-UL), and extended the S phase. Therefore, the sweet-flavored peptides prepared from DMSPHs using the multifrequency-ultrasonic treatment have significant biological activities.


Assuntos
Morus , Antioxidantes , Peptídeos , Sementes , Tecnologia , Ultrassom
6.
Food Chem ; 367: 130722, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34375893

RESUMO

Lupin is slated as a potential contributor towards future food security. Lupin possesses several nutritional and nutraceutical attributes, many linked to seed proteins. For in-depth characterisation of the lupin proteome, liquid chromatography-tandem mass spectrometry was used to evaluate four protein extraction procedures. The proteomes of three narrow-leafed lupin were qualitatively evaluated using protein/peptide identifications and further quantitatively assessed by data-independent proteome measurement. Each extraction buffer led to unique protein identifications; altogether yielding 2,760 protein identifications from lupin varieties. The analysis of protein abundance data highlighted distinct differences between Tris-HCl and urea extracted proteomes, while also revealing variation amongst the cultivar proteomes with the wild accession (P27255) distinctly different from the domesticated cultivars (Tanjil, Unicrop). The extraction buffer used influenced the proteome coverage, downstream functional annotation results and consequently the biological interpretation demonstrating the need to optimise and understand the impact of protein extraction conditions.


Assuntos
Lupinus , Lupinus/genética , Espectrometria de Massas , Folhas de Planta , Proteoma , Sementes
7.
Food Chem ; 367: 130760, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34390911

RESUMO

Rapid volatile detection methods for seed vigour rely heavily on artificial ageing (AA), however the comparability of volatile organic compounds (VOCs) to natural ageing (NA) and practicability of the detection models were not well known. In this study, VOCs between AA and NA sweet corn seeds were compared and Partial Least Squares Regression (PLS-R) modelswere constructed based on AA to predict the seed vigour of NA. A total of 33 VOCs were identified, among which aldehydes showed the highest consistency between NA and AA. Furthermore, a AS-PLS-R model with variable importance in projection (VIP > 1) and Pearson Correlation Coefficient (r > 0.9) algorithms, which was built on 3 volatile markers: benzaldehyde monomer, n-nonanal, 1-butanol monomer, achieved the best performance (R2p of 0.901 and RMSEP of 0.050). Therefore, coupling Gas Chromatography- Ion Mobility Spectrometry (GC-IMS) with chemometrics can be an effective way to monitor and predict stored seeds vigour.


Assuntos
Compostos Orgânicos Voláteis , Cromatografia Gasosa-Espectrometria de Massas , Espectrometria de Mobilidade Iônica , Análise dos Mínimos Quadrados , Sementes/química , Compostos Orgânicos Voláteis/análise
8.
J Plant Res ; 134(6): 1155-1158, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34613490

RESUMO

Plant cell walls have multiple functions, including determining cell shape and size, cell-cell adhesion, controlling cell differentiation and growth, and promoting abiotic and biotic stress tolerance. This virtual issue introduces the physiological functions of cell walls in growth and environmental responses. The articles detail research on (1) embryogenesis and seed development, (2) vegetative growth, (3) reproductive growth, and (4) environmental responses. These articles, published in the Journal of Plant Research, will provide valuable information for future research on the function and dynamics of cell walls at various growth stages, and in response to environmental factors.


Assuntos
Desenvolvimento Vegetal , Plantas , Parede Celular , Regulação da Expressão Gênica de Plantas , Sementes , Estresse Fisiológico
9.
Nature ; 598(7881): 397-398, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34646029
10.
Plant Physiol Biochem ; 167: 1024-1034, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34598022

RESUMO

Paphiopedilum spicerianum (P. spicerianum) is a rare orchid species with high ornamental value. Asymbiotic germination is the most efficient propagation method for conservation and commercial purposes because clonal propagation is very difficult and the separation of native species of Paphiopedilum through aseptic seeding is uncommon owing to their conservatism. However, a high protocorm developmental arresting rate during the asymbiotic germination is the major obstacle for seedling establishment. The fundamental understanding of embryo and protocorm developmental mechanisms will guide the development of an effective propagation method. The morphological and physiological characterization of the key developmental process of embryos and protocorms shows that the mature seeds of P. spicerianum consist of a spherical embryo without an endosperm. Seed coats become heavily lignified once the embryo is mature. Embryo cell size is relatively uniform, and significant structure polarity and cell size gradients occur at the early protocorm stage. The high level of auxin and cytokinin accumulation at the early stage of embryo development and protocorm stage may help to facilitate cell division. The transcriptome profiles of protocorms at three different developmental stages were compared to explore the regulatory mechanism of protocorm development. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that differentially expressed genes were implicated in secondary metabolite metabolism, plant hormone signal transduction and photosynthesis. The temporal expression patterns of candidate genes related to embryo and shoot development were analyzed to reveal their roles in protocorm development: in the early stage of protocorm development, embryonic development related genes such as SERKs and BBM1 were active, while in the late stage of protocorm, shoot apical meristem related genes such as WOX8, CLAVATA2, CUC2, and SCR were active.


Assuntos
Orchidaceae , Germinação , Orchidaceae/genética , Plântula , Sementes , Transcriptoma
11.
J Wound Care ; 30(Sup9a): XIIIi-XIIIx, 2021 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-34597172

RESUMO

OBJECTIVE: Providing a suitable environment to improve the healing process is the main target of wound dressing that also protects the wound from additional harms. In the present study, fabrication and characterisation of a new kind of electrospun wound dressing composed of polyvinyl alcohol (PVA) and quince seed mucilage (QSM) is reported. METHOD: QSM was extracted from quince seeds, purified, freeze-dried and used to produce aqueous solutions containing different amounts of PVA and QSM. The wound dressings were fabricated via the electrospinning method and their characteristics were investigated with scanning electron microscope (SEM) images, Fourier transform infrared (FTIR) spectra, tensile and swelling test, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) cytotoxicity assay against fibroblast cells. RESULTS: SEM images confirmed that proper, uniform, non-oriented nanofibres with an average diameter in the range of 60-240nm, depending on the QSM content had been fabricated. The tensile test showed that with increasing QSM content, the tensile strength of fibre increased while elongation at break was decreased, which was consistent with SEM images where the diameter of samples decreased by increasing QSM content. MTT assay showed significant biocompatibility against fibroblast cells; however, it was increased by increased QSM proportion. In addition, SEM images supported the proper adhesion of fibroblast cells on the sample one day after culturing. CONCLUSION: Overall, the findings of the current study support the potential of PVA/QSM nanofibres as a proper candidate for biomedical applications, especially as a wound dressing.


Assuntos
Álcool de Polivinil , Rosaceae , Bandagens , Sementes , Cicatrização
12.
Planta ; 254(5): 97, 2021 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-34655341

RESUMO

MAIN CONCLUSION: Overexpression of forage sorghum oleosin genes in Arabidopsis oleosin-deficient mutant and yeast showed increased germination rate, triacylglycerol content, and protection against lipase-mediated TAG degradation. Plant lipids are an important source of ration for cattle or other livestock animals to fulfil their energy needs. Poor energy containing green forages are still one of the major sources of food for livestock animals, leaving the animals undernourished. This lowers the milk and meat production efficiency, thereby affecting human consumption. Oleosin, an essential oil body surface protein, is capable of enhancing and stabilizing the lipid content in plants. We identified and functionally characterized three forage sorghum oleosin genes (SbOle1, SbOle2, and SbOle3) in Arabidopsis and yeast. Phylogenetic analysis of SbOle proteins showed a close relationship with rice and maize oleosins. Expression analysis of SbOle genes determined a higher expression pattern in embryo followed by endosperm, while its expression in the non-seed tissues remained negligible. Overexpression of SbOle genes in Arabidopsis ole1-deficient mutants showed restoration of normal germination whereas control mutant seeds showed lower germination rates. Heterologous overexpression of SbOle in yeast cells resulted in increased TAG accumulation. Additionally, the TAG turnover assay showed the effectiveness of SbOle genes in reducing the yeast endogenous and rumen bacterial lipase-mediated TAG degradation. Taken together, our findings not only provide insights into forage sorghum oleosin for increasing the energy content in non-seed organs but also opened up the direction towards implication of oleosin in rumen protection of fodders.


Assuntos
Proteínas de Arabidopsis , Arabidopsis , Sorghum , Animais , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Bovinos , Filogenia , Proteínas de Plantas/genética , Saccharomyces cerevisiae/genética , Sementes/genética , Sorghum/genética , Triglicerídeos
13.
J Agric Food Chem ; 69(39): 11606-11615, 2021 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-34553917

RESUMO

Raffinose family oligosaccharides (RFOs) are accumulated during the late stage of seed development and hydrolyzed during seed germination. The process of raffinose hydrolysis during seed germination and how this process affects seed vigor remains unknown. We report here that maize alkaline α-galactosidase 1 (ZmAGA1) protein is translationally induced and is capable of hydrolyzing RFOs as well as a precursor, galactinol, during seed germination. Constitutively overexpressing ZmAGA1 in Arabidopsis decreased both RFOs and galactinol contents of mature, desiccated, and 30 hours after imbibition (HAI) seeds, yet enhanced the seed germination percentage under either salt or somewhat osmotic-stress conditions at earlier times during the time course. However, ZmAGA1 overexpression also decreased the seed aging tolerance of mature, desiccated seeds as compared with wild type (WT) or those overexpressing GFP. Compared to that of WT control seeds, the atsip2 (mutant of Arabidopsis AtSIP2 (seed imbibition protein 2, encoding alkaline α-galactosidase)) seeds have similar RFOs and galactinol contents in mature, desiccated seeds but significantly increased the amount of these metabolites at 30 HAI. This retention of RFOs and galactinol in atsip2 results in seeds that exhibit lowered seed germination percentage under either salt or osmotic stress conditions, and yet, increased seed aging tolerance relative to WT. Similarly, when maize seeds were imbibed in the presence of a specific α-galactosidase inhibitor (1-deoxygalactonojirimycin) as compared to those imbibed in water, greater amounts of raffinose and galactinol were detected; the seeds exhibited decreased seed germination percentages but increased seed aging tolerance. Taken together, these data suggest that both maize seed germination and seed aging tolerance can be simultaneously regulated through careful temporal manipulation of ZmAGA1 expression.


Assuntos
Arabidopsis , Germinação , Arabidopsis/genética , Oligossacarídeos , Rafinose , Sementes
14.
BMC Plant Biol ; 21(1): 417, 2021 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-34507551

RESUMO

BACKGROUND: The future productivity of wheat (T. aestivum L.) as the most grown crop worldwide is of utmost importance for global food security. Thousand kernel weight (TKW) in wheat is closely associated with grain architecture-related traits, e.g. kernel length (KL), kernel width (KW), kernel area (KA), kernel diameter ratio (KDR), and factor form density (FFD). Discovering the genetic architecture of natural variation in these traits, identifying QTL and candidate genes are the main aims of this study. Therefore, grain architecture-related traits in 261 worldwide winter accessions over three field-year experiments were evaluated. RESULTS: Genome-wide association analysis using 90K SNP array in FarmCPU model revealed several interesting genomic regions including 17 significant SNPs passing false discovery rate threshold and strongly associated with the studied traits. Four of associated SNPs were physically located inside candidate genes within LD interval e.g. BobWhite_c5872_589 (602,710,399 bp) found to be inside TraesCS6A01G383800 (602,699,767-602,711,726 bp). Further analysis reveals the four novel candidate genes potentially involved in more than one grain architecture-related traits with a pleiotropic effects e.g. TraesCS6A01G383800 gene on 6A encoding oxidoreductase activity was associated with TKW and KA. The allelic variation at the associated SNPs showed significant differences betweeen the accessions carying the wild and mutated alleles e.g. accessions carying C allele of BobWhite_c5872_589, TraesCS6A01G383800 had significantly higher TKW than the accessions carying T allele. Interestingly, these genes were highly expressed in the grain-tissues, demonstrating their pivotal role in controlling the grain architecture. CONCLUSIONS: These results are valuable for identifying regions associated with kernel weight and dimensions and potentially help breeders in improving kernel weight and architecture-related traits in order to increase wheat yield potential and end-use quality.


Assuntos
Genótipo , Fenótipo , Polimorfismo de Nucleotídeo Único , Sementes/anatomia & histologia , Sementes/genética , Triticum/anatomia & histologia , Triticum/genética , Grão Comestível/anatomia & histologia , Grão Comestível/genética , Variação Genética , Genoma de Planta , Estudo de Associação Genômica Ampla , Locos de Características Quantitativas
15.
J Agric Food Chem ; 69(38): 11154-11162, 2021 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-34529438

RESUMO

In this study, the enantioselective activity of the chiral fungicide triticonazole (TRZ) against target pathogens of seed-borne diseases and the effect on the early phenotypic indexes and physiological and biochemical indexes of wheat following the soaking of seeds were studied. S-TRZ had low fungicidal activity and significantly inhibited the germination of wheat seeds. Seedlings in the S-TRZ treatment group suffered severe oxidative damage; the contents of glutathione (GSH) and malondialdehyde (MDA) were significantly increased. S-TRZ significantly inhibited the synthesis of gibberellin (GA) and ethylene (ETH), while it significantly increased that of jasmonic acid (JA). R-TRZ showed the highest activity against two smut fungi; the treatment of seeds with 5 g (ai)/100 kg R-TRZ had little influence on early wheat growth. The plant hormones were synthesized normally; seedlings grew well, and the fresh weight increased significantly following this treatment. Based on these data, we propose to develop and apply R-TRZ using the national recommended doses to control seed-borne diseases of wheat.


Assuntos
Praguicidas , Triticum , Ciclopentanos , Germinação , Plântula , Sementes , Estereoisomerismo , Triazóis/toxicidade
16.
J Genet ; 1002021.
Artigo em Inglês | MEDLINE | ID: mdl-34553697

RESUMO

The Waxy locus of rice is a highly polymorphic region embedded with microsatellite repeats in the 5'UTR leader intron 1 region, 23-bp duplication (wx motif) in exon 2, SNPs in exons 4, 6 and 10, p-Sine-r2 element in intron 1 and TnR-1 element in inton 13. Of the 80 polymorphic sites detected on the Wx gene, 24 are located in p-Sine-r2 and TnR-1 elements, revealing a higher substitution rate of bases in these two regions. All the cultivars with chalky endosperm had the 5'-AGTTATA-3' haplotype in intron 1 and 'A' to 'G' substitution at ?497 in exon 4. The AAC of starch from grains of all the accessions showed strong correlation (r=0.967) with GBSS-I activity in the grains. Based on the polymorphic sites of the Waxy locus and the GBSS-I activities, six allelic variants were defined which included wx, Wxop, Wxb, Wxin, Wxa2 and Wxa1, respectively, corresponded to glutinous, very low, low, intermediate, highII and highI amylose classes. Phylogenetic tree developed from alignment matrix of nucleotide sequences of the Waxy locus identified wx, Wxb and Wxin alleles with japonica lineage of Oryza sativa and the Wxop, Wxa2 and Wxa1 with indica lineage.


Assuntos
Alelos , Amilose/metabolismo , Domesticação , Oryza/genética , Amido/metabolismo , Sequência de Bases , Regulação da Expressão Gênica de Plantas , Loci Gênicos , Repetições de Microssatélites/genética , Modelos Moleculares , Motivos de Nucleotídeos/genética , Oryza/enzimologia , Filogenia , Polimorfismo Genético , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Sementes/anatomia & histologia , Sementes/genética , Sintase do Amido/química , Sintase do Amido/genética , Sintase do Amido/metabolismo
17.
Planta ; 254(5): 87, 2021 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-34585280

RESUMO

MAIN CONCLUSION: The seed coat gene VviAGL11 coordinates with endosperm development genes FIS2, PHERESE1 and IKU2 and functions as the key regulator in seed development and abortion processes in grapevine. Seed development is essential for the reproduction of flowering plants. Seed abortion is a specific characteristic that produces seedless berries and is often observed in cultivated grapevines. Although seedlessness is an important trait for table and dried grapevine production, the mechanism of seed abortion remains poorly understood. This research aimed to analyze the co-expression of the seed coat development gene VviAGL11 and the endosperm development genes FERTILIZATION INDEPENDENT SEED2 (FIS2), PHERESE1 and HAIKU2 (IKU2) that regulate seedless fruit development in grapevine. The transcript levels of VviAGL11, FIS2, PHERESE1 and IKU2 all decreased during seed abortion in the seedless grape 'Thompson Seedless' plants, compared to those of the seeded grape 'Pinot Noir'. The transcript levels of the salicylic acid (SA)-dependent defense response genes EDS1, NPR1, NDR1 and SID2 were higher in 'Thompson Seedless' than 'Pinot Noir' during seed development. Also, WRKY3, WRKY6 and WRKY52, which participate in the SA pathway, were higher expressed in 'Thompson Seedless' than in 'Pinot Noir', indicating that SA-dependent defense responses may regulate seed abortion. The genes related to synthesis and metabolism of gibberellic acid (GA) and abscisic acid (ABA) also showed differential expression between 'Thompson Seedless' and 'Pinot Noir'. Exogenous applications of plant growth regulators (PGRs) to inflorescences of three stenospermocarpy grapevines before flowering showed that GA3 was critical prominently in seed development. Therefore, the co-expression of seed coat and endosperm development-related genes, SA pathway genes, and genes for the synthesis and metabolism of GA3 together enhance seed abortion in seedless grapes.


Assuntos
Regulação da Expressão Gênica de Plantas , Vitis , Endosperma/genética , Reprodução , Sementes/genética , Vitis/genética
18.
New Phytol ; 232(2): 476-478, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34547105

Assuntos
Germinação , Sementes
19.
Trop Anim Health Prod ; 53(5): 486, 2021 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-34586503

RESUMO

The aim of this study was to evaluate the effects of partial replacement of forage and concentrate with pomegranate pulp silage (PPS) and dried pomegranate seed pulp (PSP) on performance, dry matter intake (DMI), and carcass characteristics of fattening Mehraban lambs. Twenty-four male lambs (mean body weight 27.0 ± 3.5 kg) were fed with three isonitrogenous and isoenergetic diets (n = 8 per diet), consisting of a control diet, a PPS diet containing 27.2% pomegranate pulp silage, and a PSP diet containing 31.4% dried pomegranate seed pulp. The experimental diets were fed ad libitum as total mixed rations for 65-day fattening period, on two meals per day, and then the growth performance, feed intake, and carcass characteristics were determined. The initial BW, final BW, average daily gain (ADG), and feed conversion ratio (FCR) were not different among the experimental diets. The amount of DMI in PSP diet was higher than that in the control diet (P = 0.023) but was not different between the control and PPS diets. There was no significant difference among diets for carcass characteristics. Using PPS and PSP in the diets decreased (P < 0.05) the kidney fat, but had no impact on the heart, liver, kidneys, spleen, and lungs. The results showed that PPS and PSP could be used to replace part of the diet for fattening lambs, while decreasing the dietary cost without having any negative effects on animal performance.


Assuntos
Romã (Fruta) , Silagem , Ração Animal/análise , Animais , Sementes , Ovinos , Carneiro Doméstico , Silagem/análise
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