RESUMO
Phenolic compounds (PC) were analyzed by UHPLC-ESI-QTOF-MSE in two sorghum genotypes, harvested in two growing seasons (GS) at five distinct days after flowering (DAF) to evaluate how genotype/GS influences the PC synthesis and antioxidant capacity during grain growth. Total phenolic contents were strongly correlated with antioxidant capacity (r > 0.9, p < 0.05). Globally, 97 PC were annotated, including 20 PC found irrespective of the grain developmental stage and genotype/GS. The phenolic profile clearly differs between stages: phenolic acids were the most abundant class in early stages (50%), and flavonoid accumulation becomes predominant in late ones (3/5 of total ion abundance). Dimeric and trimeric tannins were identified even in 10DAF grains. Chemometry revealed great PC variability between genotypes (27%) and important biomarkers of GS differentiation (e.g., ferulic acid). This work can input open databases of PC and paves the way to understand biosynthetic pathways of PC in sorghum and future sorghum selection.
Assuntos
Antioxidantes , Metabolômica , Fenóis , Sorghum , Sorghum/metabolismo , Sorghum/crescimento & desenvolvimento , Sorghum/química , Sorghum/genética , Antioxidantes/metabolismo , Antioxidantes/química , Fenóis/metabolismo , Fenóis/química , Cromatografia Líquida de Alta Pressão , Sementes/crescimento & desenvolvimento , Sementes/química , Sementes/metabolismo , Sementes/genética , Extratos Vegetais/química , Extratos Vegetais/metabolismo , Genótipo , Flavonoides/metabolismo , Flavonoides/químicaRESUMO
We analyze the proteome changes during the development of the carnauba palm (Copernicia prunifera) seedlings under skotomorphogenic conditions, by separating the embryo into its two components: haustorium (HA) and cotyledonary petiole (CP) and established the descriptive and quantitative proteomes of these tissues across four developmental stages. 5205 proteins were identified in HA and 6028 in CP. These proteomes are rich in proteins known to maintain the skotomorphogenic state, and in a complete set of proteins involved in cellular respiration and biosynthesis of secondary metabolites. The quantitative analysis employing a label-free approach revealed that 583 proteins in HA and 383 in CP were differentially abundant, with 251 proteins shared between the datasets. The results showed that HA participates in the digestion of food reserves present in HA itself and in the endosperm, acting as a conduit of nitrogen and carbon sources for the growing embryo axis. Among the differentially abundant proteins in the CP, we identified the presence of proteins from the cellular metabolism and proteins involved in the hydrolysis of food reserves such as starch and proteins. This indicates that the CP, in addition to the endosperm and HA, serves as a source of food reserves for the embryo axis. SIGNIFICANCE: Our results also reveal the differential regulation of specific proteins involved in reactive oxygen species scavenging, cell wall remodeling, respiratory metabolism, and protein repair in seeds and seedlings of C. prunifera. These findings have broad implications for understanding the energy metabolism that drives the transition from seed to seedling. For this study, we employed state-of-the-art proteomic techniques, including quantitative mass spectrometry and bioinformatic analysis, that allowed us to create a large dataset that will be a valuable resource for future research on the physiological and biochemical aspects of skotomorphogenesis, photomorphogenesis, and the transition between these states.
Assuntos
Arecaceae , Germinação , Proteínas de Plantas , Proteoma , Plântula , Proteoma/metabolismo , Proteínas de Plantas/metabolismo , Plântula/metabolismo , Plântula/crescimento & desenvolvimento , Arecaceae/metabolismo , Arecaceae/crescimento & desenvolvimento , Germinação/fisiologia , Proteômica/métodos , Sementes/metabolismo , Sementes/crescimento & desenvolvimentoRESUMO
Cafestol is an ent-kaurene skeleton diterpene that is present in coffee beans and brews. Although several biological activities have been described in the literature for cafestol, such as hypercholesterolemic, anti-inflammatory, anticerous, and antidiabetic effects, its metabolism within the human body remains poorly understood. Therefore, this study aimed to quantify cafestol in boiled coffee brew, assess its bioaccessibility using a static in vitro digestion model, and investigate the metabolites formed during the digestion process using liquid chromatography coupled to high-resolution mass spectrometry. Cafestol content in the boiled coffee brew ranged from 127.47 to 132.65 mg L-1. The bioaccessibility of cafestol from boiled coffee brew using the in vitro digestion model was 93.65%; additionally, in the intestinal phase, cafestol was mainly found in its alcohol form. Additionally, a novel carboxylic acid derivative metabolite from cafestol with m/z 331.1909 [M + H]+ formed in the oral digestion phase is proposed. This metabolite was also detected in other digestion phases. Thus, this is the first article to investigate the metabolism of cafestol during digestion using an in vitro digestion model. The results indicate that cafestol is bioaccessible, is available to absorption, in its alcohol form, and suffers an oxidation reaction during the oral phase of digestion.
Assuntos
Coffea , Café , Digestão , Espectrometria de Massas , Modelos Biológicos , Humanos , Coffea/química , Coffea/metabolismo , Café/química , Café/metabolismo , Espectrometria de Massas/métodos , Sementes/química , Sementes/metabolismo , Diterpenos/metabolismo , Diterpenos/química , Diterpenos/análise , Diterpenos/farmacocinética , Disponibilidade Biológica , Cromatografia Líquida de Alta Pressão , CulináriaRESUMO
Plants have developed various strategies to deal with abiotic stresses throughout their lifetimes. However, environmental stresses can have long-lasting effects, positively modifying plant physiological responses to subsequent stress episodes, a phenomenon known as preconditioning or stress memory. Intriguingly, this memory can even be transmitted to offspring, referred to as "inter- or transgenerational memory". Chenopodium quinoa is a pseudocereal that can withstand several abiotic stresses, including nitrogen (N) limitation. This research highlights the critical role of maternal N conditions in shaping the physiological and metabolic responses of their offspring. Mother quinoa plants (F0) were grown under High N (HN) or Low N (LN) conditions. LNF0 plants exhibited lower panicle biomass, net photosynthesis, and yield compared to HNF0 plants. Seeds from LNF0 retained proteins, reduced amino acids' levels, and increased lipids (such as PI 34:2), especially phosphatidylcholines, and their unsaturation level, which was associated with faster germination compared to HNF0 seeds. Offsprings seedlings (F1) grown under either HN or LN had similar proteins and amino acid proportions of their seeds. However, LNF0LNF1 seedlings displayed significantly higher biomass and number of root tips. These changes were significantly correlated with transpiration, net photosynthesis, and stomatal conductance, as well as with starch content, suggesting higher CO2 fixation at the whole plant level in LNF0LNF1 plants. Our findings suggest that quinoa transmits maternal environmental stress information to its offspring, modulating their resilience. This work underscores the potential of utilizing maternal environmental conditions as a natural priming tool to enhance crop resilience against nutritional stress.
Assuntos
Chenopodium quinoa , Nitrogênio , Sementes , Estresse Fisiológico , Chenopodium quinoa/fisiologia , Chenopodium quinoa/metabolismo , Chenopodium quinoa/crescimento & desenvolvimento , Nitrogênio/metabolismo , Sementes/fisiologia , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Fotossíntese/fisiologia , Plântula/fisiologia , Plântula/crescimento & desenvolvimento , Plântula/metabolismo , Biomassa , Germinação/fisiologiaRESUMO
OBJECTIVES: In vitro seed propagation can enhance plant species growth and enable the rapid production of seedlings while preserving genetic variability. This study aimed to develop in vitro seed propagation and acclimatization protocols for Dyckia rariflora to support conservation efforts of this bromeliad endemic to ferruginous campos rupestres. Seed germination and plant growth were tested using MS (Murashige & Skoog) culture medium with varying salt concentrations, sucrose levels, and the presence or absence of polyvinylpyrrolidone (PVP). Following these treatments, seedlings were acclimatized after removal from the controlled environment. RESULTS: Germination rates varied between 65 and 90%, unaffected by treatment. The highest germination speed index was in half MS salts without PVP, while full MS salts, sucrose, and PVP slowed germination. Half MS salts resulted in seedlings with greater height, more leaves, and longer roots. Complete MS salts were less effective. No seed oxidation was observed. After 120 days of acclimatization, survival rates exceeded 70%, with plants in half MS salts and 15 g sucrose showing the best growth. In vitro propagation of D. rariflora is viable for large-scale plant production, with half MS salt and sucrose concentrations, without PVP, recommended for better plant growth and cost reduction.
Assuntos
Bromeliaceae , Germinação , Plântula , Sementes , Plântula/crescimento & desenvolvimento , Plântula/metabolismo , Germinação/efeitos dos fármacos , Bromeliaceae/metabolismo , Bromeliaceae/crescimento & desenvolvimento , Bromeliaceae/genética , Sementes/crescimento & desenvolvimento , Sementes/efeitos dos fármacos , Sementes/metabolismo , Espécies em Perigo de Extinção , Conservação dos Recursos Naturais/métodos , Sacarose/metabolismo , Aclimatação , Meios de Cultura/química , PovidonaRESUMO
Cocoa (Theobroma cacao L.) is the basic raw material to produce chocolate and other derivatives such as cocoa butter, cocoa powder and cocoa liquor (cocoa paste), which requires a fermentation process that affects its chemical composition and sensory profile. The objective of this study was to monitor the biochemical, physical and sensory changes during fermentation of cocoa beans in cocoa bean processing plants in the department of Caquetá, Colombia. During fermentation, the temperature of the mass and the pH of the pulp and beans were monitored at the different cocoa bean processing plants (Sites ASOACASAN ASA, COMICACAO CMI, COMCAP COC). Also, at two points during fermentation (days 4 and 7), physical properties of the bean were determined, such as variables related to bromatological composition, polyphenolic compounds and antioxidant activity as sensory attributes at the different sites. An increase in dough temperature was found, however the pH of the cotyledon decreased during the fermentation process and the fat and moisture content varied with fermentation time. At the site level, total polyphenol content (TPC), total flavonoids (TF), 1,1-diphenyl-2-picrylhydrazil (DPPH) and ferric reducing antioxidant power (FRAP) contents were statistically different, with COC being different from the other sites. The TPC was higher at the COC site (507 mg gallic acid equivalent GAE/g Cocoa) with respect to the other sites (< 360 mg GAE/g Cocoa). The TF content followed a similar behavior to TPC, with significant differences between sites and differences between fermentation times for ASA. The TF was higher in COC (309.1 mg catechin/g cocoa) with respect to CMI (215.6 mg catechin/g cocoa) and ASA (185.7 mg catechin/g cocoa). Values in DPPH ranged from 5869.3 to 7781.8 µmol Trolox/g cocoa and for the FRAP assay ranged from 369.8 to 606.7 mg ascorbic acid AA/g cocoa among the sites. It was found that the time and management of the fermentation process has a significant impact on the parameters (biochemical, physical and sensory) of cocoa beans. Therefore, it is necessary to standardize the fermentation process to achieve a quality product that meets the needs of the market.
Assuntos
Antioxidantes , Cacau , Fermentação , Polifenóis , Cacau/química , Cacau/metabolismo , Colômbia , Polifenóis/análise , Polifenóis/metabolismo , Antioxidantes/análise , Antioxidantes/metabolismo , Humanos , Flavonoides/análise , Flavonoides/metabolismo , Concentração de Íons de Hidrogênio , Paladar , Sementes/química , Sementes/metabolismo , Chocolate/análiseRESUMO
The Andean domesticated common beans (Phaseolus vulgaris) are significant sources of phenolic compounds associated with health benefits. However, the regulation of biosynthesis of these compounds during bean seed development remains unclear. To elucidate the gene expression patterns involved in the regulation of the flavonoid pathway, we conducted a transcriptome analysis of two contrasting Chilean varieties, Negro Argel (black bean) and Coscorron (white bean), at three developmental stages associated with seed color change, as well as different flavonoid compound accumulations. Our study reveals that phenolic compound synthesis initiates during seed filling, although it exhibits desynchronization between both varieties. We identified 10,153 Differentially Expressed Genes (DEGs) across all comparisons. The KEGG pathway 'Flavonoid biosynthesis' showed enrichment of induced DEGs in Negro Argel (PV172), consistent with the accumulation of delphinidin, petunidin, and malvidin hexosides in their seeds, while catechin glucoside, procyanidin and kaempferol derivatives were predominantly detected in Coscorrón (PV24). Furthermore, while the flavonoid pathway was active in both varieties, our results suggest that enzymes involved in the final steps, such as ANS and UGT, were crucial, inducing anthocyanin formation in Negro Argel. Additionally, during active anthocyanin biosynthesis, the accumulation of reserve proteins or those related to seed protection and germination was induced. These findings provide valuable insights and serve as a guide for plant breeding aimed at enhancing the health and nutritional properties of common beans.
Assuntos
Flavonoides , Perfilação da Expressão Gênica , Phaseolus , Sementes , Sementes/genética , Sementes/metabolismo , Sementes/crescimento & desenvolvimento , Phaseolus/genética , Phaseolus/metabolismo , Flavonoides/biossíntese , Flavonoides/metabolismo , Regulação da Expressão Gênica de Plantas , TranscriptomaRESUMO
Thevetia thevetioides is a species within the Apocynaceae family known for containing cardenolide-glycosides, commonly referred to as cardiac glycosides, which are characteristic of this genus. The seeds of the Thevetia species are frequently used as a model source for studying cardiac steroids, as these glycosides can be more readily extracted from the oil-rich seeds than from the plant's green tissues. In this work, the cardenolide profile of ripe and immature seeds was determined and compared to establish the main differences. Ripe seeds contain six related cardenolides and triosides, with thevetin B being the predominant component. In contrast, immature seeds exhibit a total of thirteen cardiac glycosides, including monoglycosides such as neriifolin and peruvosides A, B, and C, as well as diglycosides like thevebiosides A, B, and C. Some of these compounds have previously been identified as degradation products of more complex cardiac glycosides; however, their presence in immature seeds, as described in this study, suggests that they may serve as biosynthetic precursors to the triosides observed in mature seeds. The glycoside patterns observed via HPTLC are associated with specific chemical structures characteristic of this genus, typically featuring thevetose or acetyl-thevetose at the first position, followed by glucose or gentibiose in di- or trisaccharides, independent of the trioside aglycones identified: digitoxigenin, cannogenin, or yccotligenin. Ripe seeds predominantly contain triosides, including thevetin B, C, and A, the latter of which has not been previously reported.
Assuntos
Cardenolídeos , Glicosídeos Cardíacos , Sementes , Espectrometria de Massas em Tandem , Sementes/química , Sementes/metabolismo , Cardenolídeos/metabolismo , Cardenolídeos/química , Glicosídeos Cardíacos/química , Glicosídeos Cardíacos/metabolismo , Espectrometria de Massas em Tandem/métodos , Cromatografia em Camada Fina/métodos , Vias Biossintéticas , Apocynaceae/química , Apocynaceae/metabolismoRESUMO
There is a limited number of studies analyzing the molecular and biochemical processes regulating the metabolism of the maturation of Cocos nucifera L. zygotic embryos. Our research focused on the regulation of carbohydrate and lipid metabolic pathways occurring at three developmental stages of embryos from the Mexican Pacific tall (MPT) and the Yucatan green dwarf (YGD) cultivars. We used the TMT-synchronous precursor selection (SPS)-MS3 strategy to analyze the dynamics of proteomes from both embryos; 1044 and 540 proteins were determined for the MPT and YGD, respectively. A comparison of the differentially accumulated proteins (DAPs) revealed that the biological processes (BP) enriched in the MPT embryo included the glyoxylate and dicarboxylate metabolism along with fatty acid degradation, while in YGD, the nitrogen metabolism and pentose phosphate pathway were the most enriched BPs. Findings suggest that the MPT embryos use fatty acids to sustain a higher glycolytic/gluconeogenic metabolism than the YGD embryos. Moreover, the YGD proteome was enriched with proteins associated with biotic or abiotic stresses, e.g., peroxidase and catalase. The goal of this study was to highlight the differences in the regulation of carbohydrate and lipid metabolic pathways during the maturation of coconut YGD and MPT zygotic embryos.
Assuntos
Metabolismo dos Carboidratos , Cocos , Ácidos Graxos , Proteínas de Plantas , Sementes , Ácidos Graxos/metabolismo , Proteínas de Plantas/metabolismo , Sementes/metabolismo , Sementes/crescimento & desenvolvimento , Cocos/metabolismo , Proteômica/métodos , Proteoma/metabolismo , Metabolismo dos Lipídeos , Regulação da Expressão Gênica de PlantasRESUMO
Seed water imbibition is critical to seedling establishment in tropical forests. The seeds of the neotropical tree Hymenaea courbaril have no oil reserves and have been used as a model to study storage cell wall polysaccharide (xyloglucan - XyG) mobilization. We studied pathways of water imbibition in Hymenaea seeds. To understand seed features, we performed carbohydrate analysis and scanning electron microscopy. We found that the seed coat comprises a palisade of lignified cells, below which are several cell layers with cell walls rich in pectin. The cotyledons are composed mainly of storage XyG. From a single point of scarification on the seed surface, we followed water imbibition pathways in the entire seed using fluorescent dye and NMRi spectroscopy. We constructed composites of cellulose with Hymenaea pectin or XyG. In vitro experiments demonstrated cell wall polymer capacity to imbibe water, with XyG imbibition much slower than the pectin-rich layer of the seed coat. We found that water rapidly crosses the lignified layer and reaches the pectin-rich palisade layer so that water rapidly surrounds the whole seed. Water travels very slowly in cotyledons (most of the seed mass) because it is imbibed in the XyG-rich storage walls. However, there are channels among the cotyledon cells through which water travels rapidly, so the primary cell walls containing pectins will retain water around each storage cell. The different seed tissue dynamic interactions between water and wall polysaccharides (pectins and XyG) are essential to determining water distribution and preparing the seed for germination.
Assuntos
Parede Celular , Cotilédone , Hymenaea , Pectinas , Polissacarídeos , Sementes , Água , Hymenaea/metabolismo , Parede Celular/metabolismo , Parede Celular/ultraestrutura , Parede Celular/química , Sementes/ultraestrutura , Sementes/metabolismo , Polissacarídeos/metabolismo , Água/metabolismo , Cotilédone/metabolismo , Pectinas/metabolismo , Glucanos/metabolismo , Xilanos/metabolismo , Celulose/metabolismo , Microscopia Eletrônica de VarreduraRESUMO
DNA glycosylases initiate the base excision repair (BER) pathway by catalyzing the removal of damaged or mismatched bases from DNA. The Arabidopsis DNA glycosylase methyl-CpG-binding domain protein 4 like (MBD4L) is a nuclear enzyme triggering BER in response to the genotoxic agents 5-fluorouracil and 5-bromouracil. To date, the involvement of MBD4L in plant physiological processes has not been analyzed. To address this, we studied the enzyme functions in seeds. We found that imbibition induced the MBD4L gene expression by generating two alternative transcripts, MBD4L.3 and MBD4L.4. Gene activation was stronger in aged than in non-aged seeds. Seeds from mbd4l-1 mutants displayed germination failures when maintained under control or ageing conditions, while 35S:MBD4L.3/mbd4l-1 and 35S:MBD4L.4/mbd4l-1 seeds reversed these phenotypes. Seed nuclear DNA repair, assessed by comet assays, was exacerbated in an MBD4L-dependent manner at 24 h post-imbibition. Under this condition, the BER genes ARP, APE1L, and LIG1 showed higher expression in 35S:MBD4L.3/mbd4l-1 and 35S:MBD4L.4/mbd4l-1 than in mbd4l-1 seeds, suggesting that these components could coordinate with MBD4L to repair damaged DNA bases in seeds. Interestingly, the ATM, ATR, BRCA1, RAD51, and WEE1 genes associated with the DNA damage response (DDR) pathway were activated in mbd4l-1, but not in 35S:MBD4L.3/mbd4l-1 or 35S:MBD4L.4/mbd4l-1 seeds. These results indicate that MBD4L is a key enzyme of a BER cascade that operates during seed imbibition, whose deficiency would cause genomic damage detected by DDR, generating a delay or reduction in germination.
Assuntos
Proteínas de Arabidopsis , Arabidopsis , DNA Glicosilases , Reparo do DNA , Germinação , Sementes , Sementes/genética , Sementes/metabolismo , Arabidopsis/genética , Arabidopsis/metabolismo , Proteínas de Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , DNA Glicosilases/metabolismo , DNA Glicosilases/genética , Regulação da Expressão Gênica de Plantas , Dano ao DNARESUMO
This study aimed to evaluate the effect of extrusion and of open-pan cooking on whole germinated and non-germinated grains of pearl millet (Pennisetum glaucum L. R. Br.), on its chemical-nutritional composition and in vitro iron bioavailability. The experimental design consisted of three flours: non-germination open-pan cooked millet flour (NGOPCMF), germination open-pan cooked millet flour (GOPCMF), and extrusion cooked millet flour (ECMF). The ECMF increased the carbohydrates, iron, manganese, diosmin, and cyanidin and decreased the total dietary fiber, resistant starch, lipids, and total vitamin E, in relation to NGOPCMF. The GOPCMF increased the lysine and vitamin C and decreased the phytate, lipids, total phenolic, total vitamin E, and riboflavin concentration, in relation to NGOPCMF. Furthermore, germinated cooked millet flour and extruded millet flour improved iron availability in vitro compared to non-germinated cooked millet flour. GOPCMF and ECMF generally preserved the chemical-nutritional composition of pearl millet and improved in vitro iron bioavailability; therefore, they are nutritionally equivalent and can be used to develop pearl millet-based products.
Assuntos
Disponibilidade Biológica , Culinária , Farinha , Germinação , Ferro , Pennisetum , Pennisetum/química , Pennisetum/metabolismo , Pennisetum/crescimento & desenvolvimento , Ferro/análise , Ferro/metabolismo , Farinha/análise , Valor Nutritivo , Sementes/química , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Fibras na Dieta/análise , Fibras na Dieta/metabolismoRESUMO
The research aimed to assess the effects of incorporating germinated Lupinus angustifolius flour into corn extrudates for different periods (3, 5, and 7 days), focusing on starch digestibility, morphological structure, thermal, and pasting properties. Extrudate with germinated lupinus flour for 7 days (EG7) significantly increased the content of slowly digestible starch up to 10.56% (p < 0.05). Crystallinity increased up to 20% in extrudates with germinated flour compared to extrudates with ungerminated flour (EUG), observing changes at the molecular level by FTIR that impact the thermal and pasting properties. X-ray diffraction revealed angles of 2θ = 11.31, 16.60, 19.91, and 33.04 as a result of the germination and extrusion processes. Microstructural analysis indicated starch-protein interactions influencing changes in calorimetry, viscosity, X-ray diffraction, and digestibility. PCA allowed establishing that the addition of germinated flours significantly affected the properties and microstructural characteristics of extruded products, potentially affecting digestibility and nutritional quality.
Assuntos
Digestão , Germinação , Lupinus , Amido , Difração de Raios X , Zea mays , Zea mays/química , Zea mays/crescimento & desenvolvimento , Zea mays/metabolismo , Lupinus/química , Lupinus/metabolismo , Lupinus/crescimento & desenvolvimento , Amido/química , Amido/metabolismo , Farinha/análise , Viscosidade , Sementes/química , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Manipulação de AlimentosRESUMO
Enhancing the initial stages of plant growth by using polymeric gels for seed priming presents a significant challenge. This study aimed to investigate a microgel derived from polyetheramine-poly(propylene oxide) (PPO) and a bisepoxide (referred to as micro-PPO) as a promising alternative to optimize the seed germination process. The micro-PPO integrated with an iron micronutrient showed a positive impact on seed germination compared with control (Fe solutions) in which the root length yield improved up to 39%. Therefore, the element map by synchrotron-based X-ray fluorescence shows that the Fe intensities in the seed primers with the micro-PPO-Fe gel are about 3-fold higher than those in the control group, leading to a gradual distribution of Fe species through most internal embryo tissues. The use of micro-PPO for seed priming underscores their potential for industrial applications due to the nontoxicity results in zebrafish assays and environmentally friendly synthesis of the water-dispersible monomers employed.
Assuntos
Aminas , Cucumis sativus , Germinação , Ferro , Microgéis , Sementes , Germinação/efeitos dos fármacos , Sementes/química , Sementes/metabolismo , Sementes/crescimento & desenvolvimento , Sementes/efeitos dos fármacos , Cucumis sativus/metabolismo , Cucumis sativus/crescimento & desenvolvimento , Cucumis sativus/química , Ferro/metabolismo , Ferro/química , Aminas/química , Aminas/metabolismo , Microgéis/química , Compostos de Epóxi/química , Compostos de Epóxi/metabolismo , Peixe-Zebra/metabolismo , AnimaisRESUMO
Assessing the bacterial community composition across cacao crops is important to understand its potential role as a modulator of cadmium (Cd) translocation to plant tissues under field conditions; Cd mobility between soil and plants is a complex and multifactorial problem that cannot be captured only by experimentation. Although microbes have been shown to metabolize and drive the speciation of Cd under controlled conditions, regardless of the link between soil bacterial community (SBC) dynamics and Cd mobilization in the rhizosphere, only a few studies have addressed the relationship between soil bacterial community composition (SBCC) and Cd content in cacao seeds (Cdseed). Therefore, this study aimed to explore the association between SBCC and different factors influencing the distribution of Cd across cacao crop systems. This study comprised 225 samples collected across five farms, where we used an amplicon sequencing approach to characterize the bacterial community composition. The soil Cd concentration alone (Cdsoil) was a poor predictor of Cdseed. Still, we found that this relationship was more apparent when the variation within farms was controlled, suggesting a role of heterogeneity within farms in modulating Cd translocation and, thus, seed Cd content. Our results provide evidence of the link between soil bacterial communities and the distribution of Cd across Colombian cacao crops, and highlight the importance of incorporating fine-spatial-scale studies to advance the understanding of factors driving Cd uptake and accumulation in cacao plants. IMPORTANCE: Cadmium (Cd) content in cacao crops is an issue that generates interest due to the commercialization of chocolate for human consumption. Several studies provided evidence about the non-biological factors involved in its translocation into the cacao plant. However, factors related to this process, including soil bacterial community composition (SBCC), still need to be addressed. It is well known that soil microbiome could impact compounds' chemical transformation, including Cd, on the field. Here, we found the first evidence of the link between soil bacterial community composition and Cd concentration in cacao soils and seeds. It highlights the importance of including the variation of bacterial communities to assess the factors driving the Cd translocation into cacao seeds. Moreover, the results highlight the relevance of the spatial heterogeneity within and across cacao farms, influencing the variability of Cd concentrations.
Assuntos
Bactérias , Cacau , Cádmio , Produtos Agrícolas , Microbiota , Rizosfera , Sementes , Microbiologia do Solo , Poluentes do Solo , Cádmio/metabolismo , Cádmio/análise , Cacau/microbiologia , Cacau/metabolismo , Bactérias/classificação , Bactérias/genética , Bactérias/metabolismo , Bactérias/isolamento & purificação , Poluentes do Solo/metabolismo , Poluentes do Solo/análise , Colômbia , Produtos Agrícolas/microbiologia , Produtos Agrícolas/metabolismo , Sementes/microbiologia , Sementes/metabolismo , Solo/químicaRESUMO
Plants synchronize their growth and development with environmental changes, which is critical for their survival. Among their life cycle transitions, seed germination is key for ensuring the survival and optimal growth of the next generation. However, even under favorable conditions, often germination can be blocked by seed dormancy, a regulatory multilayered checkpoint integrating internal and external signals. Intricate genetic and epigenetic mechanisms underlie seed dormancy establishment, maintenance, and release. In this review, we focus on recent advances that shed light on the complex mechanisms associated with physiological dormancy, prevalent in seed plants, with Arabidopsis thaliana serving as a model. Here, we summarize the role of multiple epigenetic regulators, but with a focus on histone modifications such as acetylation and methylation, that finely tune dormancy responses and influence dormancy-associated gene expression. Understanding these mechanisms can lead to a better understanding of seed biology in general, as well as resulting in the identification of possible targets for breeding climate-resilient plants.
Assuntos
Arabidopsis , Epigênese Genética , Histonas , Dormência de Plantas , Processamento de Proteína Pós-Traducional , Arabidopsis/genética , Arabidopsis/fisiologia , Arabidopsis/metabolismo , Arabidopsis/crescimento & desenvolvimento , Dormência de Plantas/genética , Histonas/metabolismo , Histonas/genética , Sementes/crescimento & desenvolvimento , Sementes/genética , Sementes/fisiologia , Sementes/metabolismo , Regulação da Expressão Gênica de Plantas , Proteínas de Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , GerminaçãoRESUMO
Cereal grains play an important role in human health as a source of macro- and micronutrients, besides phytochemicals. The metabolite diversity was investigated in cereal crops and their milling fractions by untargeted metabolomics ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) of 69 samples: 7 species (barley, oat, pearl millet, rye, sorghum, triticale, and wheat), 23 genotypes, and 4 milling fractions (husk, bran, flour, and wholegrain). Samples were also analyzed by in vitro antioxidant activity. UHPLC-MS/MS signals were processed using XCMS, and metabolite annotation was based on SIRIUS and GNPS libraries. Bran and husk showed the highest antioxidant capacity and phenolic content/diversity. The major metabolite classes were phenolic acids, flavonoids, fatty acyls, and organic acids. Sorghum, millet, barley, and oats showed distinct metabolite profiles, especially related to the bran fraction. Molecular networking and chemometrics provided a comprehensive insight into the metabolic profiling of cereal crops, unveiling the potential of coproducts and super cereals such as sorghum and millet as sources of polyphenols.
Assuntos
Antioxidantes , Grão Comestível , Espectrometria de Massas em Tandem , Antioxidantes/metabolismo , Antioxidantes/química , Antioxidantes/análise , Grão Comestível/química , Grão Comestível/metabolismo , Cromatografia Líquida de Alta Pressão , Sorghum/química , Sorghum/metabolismo , Avena/química , Avena/metabolismo , Avena/genética , Triticum/química , Triticum/metabolismo , Triticum/genética , Flavonoides/metabolismo , Flavonoides/análise , Flavonoides/química , Extratos Vegetais/química , Extratos Vegetais/metabolismo , Milhetes/química , Milhetes/metabolismo , Milhetes/genética , Hordeum/química , Hordeum/metabolismo , Hordeum/genética , Sementes/química , Sementes/metabolismo , Metabolômica , Produtos Agrícolas/química , Produtos Agrícolas/metabolismo , Produtos Agrícolas/genéticaRESUMO
Amaranth is a pseudocereal that contains between 50 and 60% starch, gluten-free protein, and essential amino acids. This study investigates the physicochemical changes in Amaranthus spp. grains, flour, isolated starch and nanocrystals during germination and malting. The moisture content increased from 8.9% to 41% over 2 h of soaking. The percentage of germination increased rapidly, reaching 96% after 60 h, a remarkable advantage over other cereals. The nutrient composition varied, including protein synthesis and lipid degradation. Lipid concentration decreased during malting, except for soaking, which increased by 62%. Scanning electron microscopy shows that germination does not cause morphological changes on the outer surface of the grains, while transmission electron microscopy indicates the presence of isolated nanocrystals with orthorhombic crystal structure confirmed by X-ray diffraction. The viscosity profile shows a decrease in peak viscosity. Therefore, amaranth is a potential pseudocereal that can be used as an additive in the production of fermented beverages.
Assuntos
Amaranthus , Farinha , Germinação , Nanopartículas , Amido , Amaranthus/química , Amaranthus/crescimento & desenvolvimento , Amaranthus/metabolismo , Farinha/análise , Amido/química , Amido/metabolismo , Nanopartículas/química , Viscosidade , Sementes/química , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Manipulação de AlimentosRESUMO
Fertilization with nickel (Ni) can positively affect plant development due to the role of this micronutrient in nitrogen (N) metabolism, namely, through urease and NiFe-hydrogenase. Although the application of Ni is an emerging practice in modern agriculture, its effectiveness strongly depends on the chosen application method, making further research in this area essential. The individual and combined effects of different Ni application methods-seed treatment, leaf spraying and/or soil fertilization-were investigated in soybean plants under different edaphoclimatic conditions (field and greenhouse). Beneficial effects of the Soil, Soil + Leaf and Seed + Leaf treatments were observed, with gains of 7 to 20% in biological nitrogen fixation, 1.5-fold in ureides, 14% in shoot dry weight and yield increases of up to 1161 kg ha-1. All the Ni application methods resulted in a 1.1-fold increase in the SPAD index, a 1.2-fold increase in photosynthesis, a 1.4-fold increase in nitrogenase, and a 3.9-fold increase in urease activity. Edaphoclimatic conditions exerted a significant influence on the treatments. The integrated approaches, namely, leaf application in conjunction with soil or seed fertilization, were more effective for enhancing yield in soybean cultivation systems. The determination of the ideal method is crucial for ensuring optimal absorption and utilization of this micronutrient and thus a feasible and sustainable management technology. Further research is warranted to establish official guidelines for the application of Ni in agricultural practices.
Assuntos
Fertilizantes , Glycine max , Níquel , Solo , Glycine max/crescimento & desenvolvimento , Glycine max/efeitos dos fármacos , Glycine max/metabolismo , Fertilizantes/análise , Solo/química , Urease/metabolismo , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Folhas de Planta/efeitos dos fármacos , Fixação de Nitrogênio/efeitos dos fármacos , Nitrogênio/metabolismo , Fotossíntese/efeitos dos fármacos , Sementes/crescimento & desenvolvimento , Sementes/efeitos dos fármacos , Sementes/metabolismo , Agricultura/métodosRESUMO
Chia (Salvia hispanica L.) seed (CS) and Pumpkin (Cucurbita moschata) seed (PS) are used in ruminant diets as energy sources. The current experiment studied the impact of dietary inclusion of CS and PS on nutrient intake and digestibility, milk yield, and milk composition of dairy sheep. Twelve primiparous Texel × Suffolk ewes [70 ± 5 days in milk (DIM); 0.320 ± 0.029 kg milk yield] were distributed in a 4 × 3 Latin square design and fed either a butter-based control diet [CON; 13 g/kg dry matter] or two diets with 61 g/kg DM of either CS or PS. Dietary inclusion of CS and PS did not alter live weight (p >0.1) and DM intake (p >0.1). However, compared to the CON, dietary inclusion of both CS and PS increased the digestibility of neutral detergent fiber (p <0.001) and acid detergent lignin (p < 0.001). Milk production (p = 0.001), fat-corrected milk (p < 0.001), and feed efficiency (p < 0.001) were enhanced with PS, while the highest milk protein yield (p < 0.05) and lactose yield (p < 0.001) were for CS-fed ewes. Compared to the CON diet, the ingestion of either CS and/or PS decreased (p < 0.001) the C16:0 in milk. Moreover, both CS and PS tended to enhance the content of C18:3n6 (p > 0.05) and C18:3n3 (p > 0.05). Overall short-term feeding of CS and/or PS (up to 6.1% DM of diet) not only maintains the production performance and digestibility of nutrients but also positively modifies the milk FA composition.