Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 23.853
Filtrar
1.
Braz. j. biol ; 84: e254016, 2024. tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1364529

RESUMO

The present study was conducted to isolate and characterize bacteria from water and soil sample taken from the Lahore Canal at different sites i.e. Mall Road, Mohlanwal and Khera site. Isolated bacterial strains were identified on the basis of morphological and biochemical tests. Identification was confirmed by culturing bacteria on selective media. Antibiotic resistance test was also performed to observe the resistance of bacteria against different antibiotics. Blood agar test was performed for identification of different pathogenic bacteria. The result revealed that water and soil samples of Lahore Canal Lahore from different sites were contaminated with Escherichia coli, Salmonella sp., Vibrio sp., Bacillus spp., Enterococcus sp. and Staphylococcus spp. Due to presence of these pathogens, this water is not suitable for any domestic and irrigation use. Study also revealed that water of the Lahore Canal is harmful for human health as it is contaminated with bacteria that can cause severe disease e.g., Escherichia coli can cause gastroenteritis, Bacillus spp. can cause nausea and vomiting, Enterococcus may infect urinary tract, Salmonella sp. is responsible for Bacteremia, Staphylococcus spp. can cause mild fever and Vibrio sp. can be the reason of cholera. Thus it is rendered unfit for any kind of human use even other than drinking like swimming, bathing, washing etc., until and unless some remedial measures are employed to eradicate pathogenic microorganisms by WASA and LWMS according to standards of WHO. Similarly, it is quite harmful, when and where ever it is used for irrigation without proper treatment.


O presente estudo foi realizado para isolar e caracterizar bactérias de amostras de água e solo retiradas do Canal Lahore, em Lahore, em diferentes locais, ou seja, Mall Road, Mohlanwal e Khera. As cepas bacterianas isoladas foram identificadas com base em testes morfológicos e bioquímicos. A identificação foi confirmada por cultura de bactérias em testes de meios seletivos. O teste de resistência aos antibióticos também foi realizado para observar a resistência das bactérias a diferentes antibióticos. Foi realizado o teste de ágar sangue para identificar diferentes bactérias patogênicas. O resultado revelou que amostras de água e solo do Canal Lahore, Lahore, de diferentes localidades estavam contaminadas com Escherichia coli, Salmonella sp., Vibrio sp., Bacillus spp., Enterococcus sp. e Staphylococcus spp. Por causa da presença desses patógenos, essa água não é adequada para qualquer uso doméstico e de irrigação. O estudo revelou que a água do Canal Lahore é prejudicial à saúde humana, pois está contaminada com bactérias que podem causar doenças graves, por exemplo: Escherichia coli pode ocasionar gastroenterite; Bacillus spp. pode causar náuseas e vômitos; Enterococcus sp. pode infectar o trato urinário; Salmonella sp. é responsável pela bacteremia; Staphylococcus spp. pode causar febre leve; e Vibrio sp. pode ser a razão da cólera. Assim, torna-se imprópria para uso humano, como natação, banho, lavagem etc., até que algumas medidas corretivas sejam empregadas para erradicar microrganismos patogênicos por WASA e LWMS de acordo com os padrões da OMS. Da mesma forma, é bastante prejudicial, quando usada para irrigação sem tratamento adequado.


Assuntos
Animais , Solo , Staphylococcus , Vibrio , Resistência Microbiana a Medicamentos , Amostras de Água , Enterococcus , Escherichia coli
2.
Georgian Med News ; (328-329): 154-157, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36318861

RESUMO

The purpose of the was to compare the antibiotic resistance of the oropharyngeal normoflora in children with frequent Viral respiratory infections (VRIs). Investigated were 33 children in 2016 and 33 children in 2021 who were treated in the Infectious Diseases Department of the Ternopil Regional (Ukraine) Children's Clinical Hospital. The obtained material from the oropharynx from the examined contingent was studied by a generally accepted microbiological method. Determination of antibiotic susceptibility of the selected strains was performed by the method of standard disks. Among surveyed children in 2016 year were 20 (60.60%) carriers of staphylococci, with 12 children of them actually S. aureus. In 2021 were different types of streptococci were isolated from almost half (15 patient) of the subjects: α-hemolytic - 51.60%, ß-hemolytic - 45.50% of patients with VRIs and S. aureus. -48.5 %. Analysis of antibiotic susceptibility of staphylococci and streptococci revealed a significant reduction in the number of susceptible staphylococcal strains to cephalosporin and penicillin, moderate - against aminoglycosides and chloramphenicol. Almost all of the studied microorganisms were insensitive to erythromycin. Over the past 5 years, the number of staphylococcal strains sensitive to ceftriaxone decreased by more than 50% (65.0% in 2016 and 14.3% in 2021), to chloramphenicol by 28.7%, ampicillin by 14.4%. The number of streptococci sensitive to all groups of antibiotics decreased by 3-4% 2021 compared to 2016, only to ampicillin the number of sensitive strains decreased by 16%.


Assuntos
Infecções Respiratórias , Staphylococcus aureus , Criança , Humanos , Testes de Sensibilidade Microbiana , Resistência Microbiana a Medicamentos , Antibacterianos/uso terapêutico , Streptococcus , Cloranfenicol/uso terapêutico , Staphylococcus , Infecções Respiratórias/tratamento farmacológico , Orofaringe , Ampicilina/uso terapêutico
3.
PLoS One ; 17(11): e0277753, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36409704

RESUMO

Inflammatory outcomes, including toxic anterior segment syndrome (TASS) and infectious endophthalmitis, are potentially painful, blinding complications following cataract surgery. In an in vitro pilot study, commercially available, sterile foldable intraocular lenses (IOLs) used during routine canine cataract surgery, and their packaging fluid were surveyed for the presence of bacterial DNA and/or viable (cultivable) bacteria. Swabs from IOLs and packaging fluid from three different veterinary manufacturers and three different production lots/manufacturer were collected for 16S ribosomal ribonucleic acid (rRNA) sequencing. Packaging fluid samples were collected for aerobic/capnophilic bacterial culture. Culture yielded one isolate, identified as Staphylococcus epidermidis. 16S rRNA sequencing revealed distinct brand-specific bacterial DNA profiles, conserved between IOLs and packaging fluid of all production lots within each manufacturer. The dominant taxonomy differentiating each manufacturer was annotated as Staphylococcus sp, and was a 100% match to S. epidermidis. Distinct mixtures of bacterial DNA are present and consistent in IOLs and packaging fluid depending on the manufacturer, and Staphylococcus is the dominant contributor to the bacterial DNA detected. Caralens products had a significantly lower amount of Staphylococcus spp. compared to Anvision and Dioptrix products.


Assuntos
Catarata , Lentes Intraoculares , Cães , Animais , RNA Ribossômico 16S/genética , DNA Bacteriano/genética , Projetos Piloto , Aderência Bacteriana , Staphylococcus epidermidis/genética , Staphylococcus/genética
4.
World J Microbiol Biotechnol ; 39(1): 5, 2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36346468

RESUMO

Aureocin A53 is an N-formylated antimicrobial peptide (AMP) produced by Staphylococcus aureus. Aureocin A53 has a broad spectrum of antimicrobial activity against human and animal pathogens. In the present study, its antagonistic activity was investigated towards 30 strains of S. aureus and 30 strains of Streptococcus spp. isolated from bovine mastitis cases in Brazil. Bovine mastitis is a disease that causes a major economic impact worldwide. Aureocin A53 inhibited the growth of all 60 strains tested, including multidrug-resistant streptococcal isolates and strains of S. aureus belonging to different pulsotypes. This AMP proved to be bactericidal against the six target strains randomly selected among staphylococci and streptococci, also exhibiting a lytic mode of action against the staphylococcal cells. Furthermore, it was determined that 2,048 AU/mL of the AMP were required to inhibit 99.99% of the cell growth of the strain less sensitive to aureocin A53. Aureocin A53 was not toxic to bovine mammary gland epithelial cells after a 24-h exposure and maintained its antimicrobial activity when tested in the excised-teat model against strains of S. aureus and Streptococcus agalactiae, the species responsible for most intramammary infections, not only in Brazil but in other countries as well. Therefore, the use of aureocin A53 in the development of new pharmacological products for the prophylaxis and/or treatment of bovine mastitis was considered promising.


Assuntos
Anti-Infecciosos , Mastite Bovina , Infecções Estafilocócicas , Feminino , Humanos , Bovinos , Animais , Staphylococcus aureus , Streptococcus agalactiae , Peptídeos Antimicrobianos , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/veterinária , Staphylococcus , Antibacterianos/farmacologia , Streptococcus , Anti-Infecciosos/farmacologia , Monofosfato de Adenosina/farmacologia
5.
PLoS One ; 17(11): e0276960, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36327238

RESUMO

BACKGROUND: Atopic dermatitis (AD) primarily affects the pediatric population, which is highly colonized by S. aureus. However, little is known about the genetic features of this microorganism and other staphylococcal species that colonize AD patients. OBJECTIVE: This study aimed to characterize Staphylococcus spp. isolated from the nares and skin (with and without lesion) of 30 AD and 12 non-AD Brazilian children. METHODS: Skin and nasal swabs were cultured onto mannitol salt agar, and bacterial colonies were counted and identified by matrix assisted laser desorption ionization time of flight mass spectrometry and polymerase chain reaction (PCR). Antimicrobial susceptibility was evaluated by phenotypic and genotypic tests. In S. aureus isolates, Panton-Valentine leukocidin genes were detected by PCR, and their clonality was assessed by pulsed-field gel electrophoresis and multilocus sequence typing. RESULTS: S. aureus was more prevalent in the nares (P = 0.005) and lesional skin (P = 0.0002) of children with AD, while S. hominis was more frequent in the skin of non-AD children (P < 0.0001). All children in the study, except one from each group, were colonized by methicillin-resistant coagulase-negative Staphylococcus and 24% by methicillin-resistant S. aureus. Despite the great clonal diversity of S. aureus (18 sequence types identified), most AD children (74.1%) were colonized by the same genotype in both niches. CONCLUSION: High colonization by polyclonal S. aureus isolates was found among children with AD, while S. hominis was more frequent among non-AD children. The high prevalence of methicillin-resistant staphylococcal isolates highlights the importance of continued surveillance, especially when considering empiric antibiotic therapy for the treatment of skin infections in these patients.


Assuntos
Dermatite Atópica , Staphylococcus aureus Resistente à Meticilina , Infecções Estafilocócicas , Humanos , Criança , Staphylococcus aureus/genética , Meticilina , Resistência a Meticilina , Dermatite Atópica/epidemiologia , Brasil/epidemiologia , Testes de Sensibilidade Microbiana , Infecções Estafilocócicas/microbiologia , Staphylococcus
6.
BMC Microbiol ; 22(1): 266, 2022 11 05.
Artigo em Inglês | MEDLINE | ID: mdl-36335318

RESUMO

Macrococcus caseolyticus is an opportunistic pathogen that is frequently isolated from dairy products and veterinary infections. Recent studies have reported the possibility of methicillin resistance that be transferred among staphylococcal species in foods. The present study examined the population structure, antimicrobial resistance, virulence factors, and morphology of methicillin-resistant M. caseolyticus by investigation of 94 genomes derived from both isolates in beef (n = 7) and pork (n = 2) at Shanghai and those deposited in public domain (n = 85). Phylogenetically, M. caseolyticus were divided into four clades, which each consisted of genomes isolated from continent of European countries (82.4%, n = 78), Asian countries (11.3%, n = 10), United States (4.1%, n = 4), Australia (1%, n = 1), and Sudan (1%, n = 1). The M. caseolyticus isolated from present study formed a genetically distinguished clade, which was characterized by novel alleles in the traditional 7-gene MLST scheme. Furthermore, we identified 24 AMR genes that were associated with 10 classes of antimicrobial agents in M. caseolyticus. Most AMR genes were carried by dominant plasmids such as rep7a, rep22 and repUS56. The genomes in the global clades carried significantly less AMR genes (p < 0.05) and more virulence factors (p < 0.001) than present clade. Virulence factors were detected in methicillin resistant M. caseolyticus including genes coding hemolysin, adherence, biofilm formation, exotoxin, and capsule that associated to human health and infection. Finally, as the close relative of the genus Staphylococcus, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were performed for morphological comparison that M. caseolyticus has a larger diameter and thicker cell wall compared with S. aureus ATCC 25,923. Taken together, our study suggested that M. caseolyticus mediating divergent antimicrobial resistance and virulence factors could serve as the vector for methicillin resistance habitats in foodborne microorganisms.


Assuntos
Staphylococcus aureus Resistente à Meticilina , Infecções Estafilocócicas , Bovinos , Animais , Humanos , Resistência a Meticilina/genética , Antibacterianos/farmacologia , Tipagem de Sequências Multilocus , Fatores de Virulência/genética , Staphylococcus aureus , Farmacorresistência Bacteriana/genética , Filogenia , China , Staphylococcus , Staphylococcus aureus Resistente à Meticilina/genética , Testes de Sensibilidade Microbiana
7.
Appl Environ Microbiol ; 88(22): e0121722, 2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36286507

RESUMO

Periprosthetic joint infection (PJI) after joint replacement is a major clinical issue requiring multiple surgeries and antibiotic interventions. Recent in vitro research has shown that PJI staphylococcal strains rapidly form antibiotic-resistant free-floating aggregates in the presence of bovine synovial fluid (BSF). Staphylococcal aggregates are also present in human PJI joint fluid. However, the influence of surface roughness and fluid shear on the attachment and retention of such aggregates on surfaces is not known. Our aim was to assess how surface roughness and fluid shear stress influenced the attachment and retention of Staphylococcus aureus BSF-mediated aggregates on smooth- and rough-patterned titanium in flow cells compared to nonaggregated cells. The attachment of S. aureus aggregates was significantly greater than that of single cells but was independent of surface roughness; however, on the patterned surfaces, aggregates preferentially accumulated in the grooves. Fibrous components in the BSF were also colocalized with the grooves. After a 24-h attachment-and-incubation period, different shear stresses were applied. There was significant detachment from flat surfaces at a flow rate of 1 mL/min (τw = 0.0012 Pa) but minimal detachment from the patterned surfaces, even at flow rates as high as 13.9 mL/min (τw = 0.0169 Pa). The retention of bacterial aggregates and biofilms on rough surfaces exposed to shear might be an important consideration for the location of colonization on orthopedic implants, which can have wide ranges of roughness and surface features and can influence the efficacy of shear-based debridement methods such as pulse lavage. IMPORTANCE Periprosthetic joint infections occurring after joint replacement are a major clinical problem requiring repeated surgeries and antibiotic interventions. Staphylococcus aureus is the most prominent bacterium causing most implant-related infections. S. aureus can form a biofilm, which is defined as a group of attached bacteria with the formation of an envelope that is resistant to antibiotics. The attachment and retention of these bacteria on implant surfaces are not clearly understood. Recent in vitro research investigations have shown that staphylococcal strains rapidly form aggregates in the presence of bovine synovial fluid (BSF) in the joints, which allows bacteria time to attach to the implant surface, leading to biofilm formation. Thus, in this study, we examined the attachment of aggregates on titanium surfaces with varying roughnesses and found robust bacterial attachment and retention along the ridges and grooves, which colocalized with the deposition of fibrous components present in the BSF.


Assuntos
Infecções Estafilocócicas , Staphylococcus aureus , Bovinos , Animais , Humanos , Líquido Sinovial/microbiologia , Titânio , Infecções Estafilocócicas/microbiologia , Antibacterianos/farmacologia , Staphylococcus , Bactérias
8.
Appl Environ Microbiol ; 88(21): e0114622, 2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36226992

RESUMO

Members of the Staphylococcaceae family, particularly those of the genus Staphylococcus, encompass important human and animal pathogens. We collected and characterized Staphylococcaceae strains from apparently healthy and diseased camels (n = 84) and cattle (n = 7) in Somalia and Kenya. We phenotypically characterized the strains, including their antimicrobial inhibitory concentrations. Then, we sequenced their genomes using long-read sequencing, closed their genomes, and subsequently compared and mapped their virulence- and resistance-associated gene pools. Genome-based phylogenetics revealed 13 known Staphylococcaceae and at least two novel species. East African strains of different species encompassed novel sequence types and phylogenetically distant clades. About one-third of the strains had non-wild-type MICs. They were resistant to at least one of the following antimicrobials: tetracycline, benzylpenicillin, oxacillin, erythromycin, clindamycin, trimethoprim, gentamicin, or streptomycin, encoded by tet(K), blaZ/blaARL, mecA/mecA1, msrA/mphC, salA, dfrG, aacA-aphD, and str, respectively. We identified the first methicillin- and multidrug-resistant camel S. epidermidis strain of sequence type (ST) 1136 in East Africa. The pool of virulence-encoding genes was largest in the S. aureus strains, as expected, although other rather commensal strains contained distinct virulence-encoding genes. We identified toxin-antitoxin (TA) systems such as the hicA/hicB and abiEii/abiEi families, reported here for the first time for certain species of Staphylococcaceae. All strains contained at least one intact prophage sequence, mainly belonging to the Siphoviridae family. We pinpointed potential horizontal gene transfers between camel and cattle strains and also across distinct Staphylococcaceae clades and species. IMPORTANCE Camels are a high value and crucial livestock species in arid and semiarid regions of Africa and gain importance giving the impact of climate change on traditional livestock species. Our current knowledge with respect to Staphylococcaceae infecting camels is very limited compared to that for other livestock species. Better knowledge will foster the development of specific diagnostic assays, guide promising antimicrobial treatment options, and inform about potential zoonotic risks. We characterized 84 Staphylococcaceae strains isolated from camels with respect to their antimicrobial resistance and virulence traits. We detected potentially novel Staphylococcus species, resistances to different classes of antimicrobials, and the first camel multidrug-resistant S. epidermidis strain of sequence type 1136.


Assuntos
Staphylococcus aureus Resistente à Meticilina , Infecções Estafilocócicas , Animais , Bovinos , Humanos , Camelus , Staphylococcus aureus , Infecções Estafilocócicas/veterinária , Staphylococcaceae , Testes de Sensibilidade Microbiana , Staphylococcus , Antibacterianos/farmacologia , Genômica , Quênia , Staphylococcus aureus Resistente à Meticilina/genética
9.
Microbiology (Reading) ; 168(10)2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36201337

RESUMO

Staphylococcus aureus is responsible for severe skin and respiratory infections and food poisoning, resulting in hospitalizations and high morbidity worldwide. Staphylococci have extensive virulence mechanisms and antimicrobial resistance that pose a global challenge to contain the spread of infectious outbreaks. Antimicrobials are used as growth promoters, and for prevention and treatment of infections in animals that provide us with food. The improvement of animal health is undeniable, but the selection of multidrug-resistant strains that can spread resistance genes among microorganisms is undesirable. The administration of sublethal doses of antimicrobials in farm animals causes stress to Staphylococci inducing the formation of a complex extracellular polymeric structure called biofilm. Such a structure may favor the persistence of infection by disseminating antimicrobial-resistant strains that can be consumed in contaminated food of animal origin. In ruminant mastitis and hospitals, the potential of the biofilm structure in the persistence of infections, especially those caused by S. aureus, has already been demonstrated, as well as its role as a source of resistant genes. In the meat production chain, the potential for persistent contamination by biofilm structure is evidently a worrying health risk . This review brings together studies demonstrating that biofilm production facilitates the exchange of mobile genetic elements and random mutations in S. aureus strains within the structure. This contributes to the emergence of more resistant clonal complexes and, with biofilm support, persists in the meat production chain.


Assuntos
Staphylococcus aureus Resistente à Meticilina , Infecções Estafilocócicas , Animais , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Biofilmes , Farmacorresistência Bacteriana/genética , Feminino , Carne , Testes de Sensibilidade Microbiana , Infecções Estafilocócicas/epidemiologia , Staphylococcus , Staphylococcus aureus/genética
10.
Vet Res ; 53(1): 84, 2022 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-36243811

RESUMO

Staphylococci and streptococci are common causes of intramammary infection in small ruminants, and reliable species identification is crucial for understanding epidemiology and impact on animal health and welfare. We applied MALDI-TOF MS and gap PCR-RFLP to 204 non-aureus staphylococci (NAS) and mammaliicocci (NASM) and to 57 streptococci isolated from the milk of sheep and goats with mastitis. The top identified NAS was Staphylococcus epidermidis (28.9%) followed by Staph. chromogenes (27.9%), haemolyticus (15.7%), caprae, and simulans (6.4% each), according to both methods (agreement rate, AR, 100%). By MALDI-TOF MS, 13.2% were Staph. microti (2.9%), xylosus (2.0%), equorum, petrasii and warneri (1.5% each), Staph. sciuri (now Mammaliicoccus sciuri, 1.0%), arlettae, capitis, cohnii, lentus (now M. lentus), pseudintermedius, succinus (0.5% each), and 3 isolates (1.5%) were not identified. PCR-RFLP showed 100% AR for Staph. equorum, warneri, arlettae, capitis, and pseudintermedius, 50% for Staph. xylosus, and 0% for the remaining NASM. The top identified streptococcus was Streptococcus uberis (89.5%), followed by Strep. dysgalactiae and parauberis (3.5% each) and by Strep. gallolyticus (1.8%) according to both methods (AR 100%). Only one isolate was identified as a different species by MALDI-TOF MS and PCR-RFLP. In conclusion, MALDI-TOF MS and PCR-RFLP showed a high level of agreement in the identification of the most prevalent NAS and streptococci causing small ruminant mastitis. Therefore, gap PCR-RFLP can represent a good identification alternative when MALDI-TOF MS is not available. Nevertheless, some issues remain for Staph. haemolyticus, minor NAS species including Staph. microti, and species of the novel genus Mammaliicoccus.


Assuntos
Doenças dos Bovinos , Doenças das Cabras , Mastite Bovina , Doenças dos Ovinos , Infecções Estafilocócicas , Animais , Bovinos , Feminino , Cabras , Mastite Bovina/diagnóstico , Leite , Reação em Cadeia da Polimerase/veterinária , Polimorfismo de Fragmento de Restrição , Ovinos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/veterinária , Infecções Estafilocócicas/veterinária , Staphylococcus , Streptococcus/genética
11.
Arch Microbiol ; 204(10): 657, 2022 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-36182972

RESUMO

134 bacterial strains were isolated from phumdis of Loktak Lake. Through 16S rRNA sequencing, Bacillus sp. (23, 17.1%), Staphylococcus sp. (14, 10.4%), Pseudomonas sp. (11, 8.2%) and Acinetobacter sp. (8, 5.9%) were identified as the predominant bacterial taxa of Loktak Lake. B. pumulis (12, 8.9%), S. arlettae (4, 2.9%), P. knackmussii (6, 4.4%) are the leading species of Bacillus, Staphylococcus and Pseudomonas, respectively. Similarly, A. seifertii (2, 1.4%) and A. calcoaceticus (2, 1.4%) are the common species of Acinetobacter. 75 (55.9%) bacterial strains showed the ability to hydrolyze one or more extracellular enzymes tested. Among the extracellular enzymes produced by the bacterial isolates, the presence of elastase activity cannot be underestimated, since the enzyme is involved in the process of bacterial lung infection. Phosphate solubilizing activity could be seen in 11.1% of the bacterial isolates. 27 (20.1%) of the strains shown to have antagonistic activity against one or more tested pathogens. An isolate, MRC 52 showed antagonistic activity against eleven different pathogens including carbapenem resistant E. coli which was further subjected to extraction and identification of the biomolecule exerting the antimicrobial property. Based on GC-MS analysis, the bioactive compound was identified as phenyl ethyl alcohol.


Assuntos
Acinetobacter , Anti-Infecciosos , Bacillus , Bacillus/genética , Bactérias/genética , Carbapenêmicos , Escherichia coli/genética , Etanol , Lagos/microbiologia , Elastase Pancreática , Fosfatos , Pseudomonas/genética , RNA Ribossômico 16S/genética , Staphylococcus/genética
12.
Toxins (Basel) ; 14(10)2022 10 18.
Artigo em Inglês | MEDLINE | ID: mdl-36287980

RESUMO

Bacterial secondary metabolites play a major role in the alleviation of diseases; however, the cytotoxicity of other metabolites cannot be ignored as such metabolites could be detrimental to human cells. Three Staphylococci strains Staphylococcus aureus, staphylococcus epidermidis and staphylococcus saprophyticus were used in the experiments. These strains are well known to cause hospital and community-acquired infections. Secondary metabolites from S. aureus isolated from milk of cows with clinical features of mastitis (swollen udders and the production of watery clotted milk), S. saprophyticus (ATCC 35552), and S. epidermidis (ATCC 51625) were exposed to a minimal medium then screened using Gas Chromatography High-Resolution Time-of-flight Mass Spectrometry (GC-HRTOF-MS) and identified with Nuclear Magnetic Resonance (NMR). From S. epidermidis, two compounds were isolated: oleamide and methyl palmitate; three from S. aureus, including fluoranthene, 3-methyl-2-phenyl-1H-pyrrole, and cyclo(L-Leu-L-Propyl); while S. saprophyticus yielded succinic acid, 1,2,6-hexantriol, veratramine, and 4-methyl-pentyl-amine. The secondary metabolites were tested for cytotoxicity using the Vero cell line. Fluoranthene exhibited toxicity with an LC50 of 0.0167 mg/mL to Vero cells, while the other metabolites did not. Methyl palmitate was the least toxic of all of the metabolites. The results imply that none of the compounds, except fluoranthene, pose any danger to human cells.


Assuntos
Infecções Estafilocócicas , Staphylococcus , Chlorocebus aethiops , Feminino , Bovinos , Humanos , Animais , Staphylococcus/metabolismo , Staphylococcus aureus , Células Vero , Ácido Succínico/metabolismo , Infecções Estafilocócicas/microbiologia , Leite/microbiologia , Staphylococcus epidermidis , Aminas , Pirróis
14.
Molecules ; 27(20)2022 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-36296701

RESUMO

Myrtaceae family is a continuous source of antimicrobial agents. In the search for novel antimicrobial agents against Staphylococcus species, bioactive fractions of Myrtus communis L., growing in the Sardinia island (Italy) have been investigated. Their phytochemical analysis led us to isolate and characterize four alkylphloroglucinol glycosides (1-4), three of them gallomyrtucommulones G-H (1,2), and myrtucommulonoside (4) isolated and characterized for the first time. The structures of the new and known compounds, endopreroxide G3 (5), myricetin-3-O-glycosides (6,7) were determined based on the spectroscopic evidence including 1D-/2D-NMR and HR-MS spectrometry. Enriched fractions as well as pure compounds were tested for their antimicrobial activity by broth micro-dilution assay against Staphylococcus epidermidis and S. aureus. Results reported herein demonstrated that gallomyrtucommulone G (1) showed a selective antimicrobial activity against both S. aureus strains (ATCC 29213 and 43300) until 16 µg/mL while gallomyrtucommulone D (3) showed the best growth inhibition value at 64 µg/mL.


Assuntos
Anti-Infecciosos , Myrtus , Myrtus/química , Floroglucinol/química , Staphylococcus aureus , Staphylococcus , Glicosídeos/farmacologia , Glicosídeos/análise , Testes de Sensibilidade Microbiana , Folhas de Planta/química , Compostos Fitoquímicos/farmacologia , Compostos Fitoquímicos/análise , Anti-Infecciosos/análise , Antibacterianos/química , Extratos Vegetais/química
15.
Front Immunol ; 13: 999201, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36189200

RESUMO

In contrast to the virulent human skin commensal Staphylococcus aureus, which secretes a plethora of toxins, other staphylococci have much reduced virulence. In these species, commonly the only toxins are those of the phenol-soluble modulin (PSM) family. PSMs are species-specific and have only been characterized in a limited number of species. S. xylosus is a usually innocuous commensal on the skin of mice and other mammals. Prompted by reports on the involvement of PSMs in atopic dermatitis (AD) and the isolation of S. xylosus from mice with AD-like symptoms, we here identified and characterized PSMs of S. xylosus with a focus on a potential involvement in AD phenotypes. We found that most clinical S. xylosus strains produce two PSMs, one of the shorter α- and one of the longer ß-type, which were responsible for almost the entire lytic and pro-inflammatory capacities of S. xylosus. Importantly, PSMα of S. xylosus caused lysis and degranulation of mast cells at degrees higher than that of S. aureus δ-toxin, the main PSM previously associated with AD. However, S. xylosus did not produce significant AD symptoms in wild-type mice as opposed to S. aureus, indicating that promotion of AD by S. xylosus likely requires a predisposed host. Our study indicates that non-specific cytolytic potency rather than specific interaction underlies PSM-mediated mast cell degranulation and suggest that the previously reported exceptional potency of δ-toxin of S. aureus is due to its high-level production. Furthermore, they suggest that species that produce cytolytic PSMs, such as S. xylosus, all have the capacity to promote AD, but a high combined level of PSM cytolytic potency is required to cause AD in a non-predisposed host.


Assuntos
Toxinas Bacterianas , Staphylococcus aureus , Animais , Toxinas Bacterianas/genética , Humanos , Mamíferos , Camundongos , Staphylococcus
16.
Braz J Microbiol ; 53(4): 2215-2222, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36074251

RESUMO

This research aimed to identify the diversity of bacterial species of the genus Staphylococcus spp. in subclinical mastitis in dairy herds in the state of Piauí, Northeastern Brazil, and to evaluate the phenotypic and genotypic resistance profile. Samples were obtained from a total of 17 dairy farms, amounting to 321 positive samples in the California Mastitis Test. Staphylococcus spp. were identified by matrix-assisted laser desorption ionization time-of-flight mass spectroscopy. Subsequently, an antibiogram was performed, and a polymerase chain reaction was carried out to screen for resistance genes in the isolates. Among all the isolates, 59.45% (110/185) belonged to the Staphylococcus genus. Moreover, the following Staphylococcus spp. were identified Staphylococcus aureus, 68.1% (75/110); Staphylococcus chromogenes, 12.7% (14/110); Staphylococcus epidermidis, 5.4% (6/110); Staphylococcus sciuri, 4.5% (5/110); Staphylococcus warneri, 2.7% (3/110); Staphylococcus haemolyticus, 1.8% (2/110); Staphylococcus hominis, 1.8% (2/110); Staphylococcus arlettae, 0.9% (1/110); Staphylococcus capitis, 0.9% (1/110); and Staphylococcus gallinarum, 0.9% (1/110). The antibiogram showed a high frequency of resistance to penicillin and ampicillin, 70.0% (77/110) and 61.8% (68/110), respectively, and a low frequency of resistance to gentamicin and vancomycin, 10.9% (12/110) and 11.8% (13/110), respectively. In the genotypic tests for the different species of Staphylococcus spp., the occurrence of the blaZ gene was observed in 60.9% (67/110) of the isolates, followed by tetL and tetM, both with 20.0% (22/110) each, and the mecA and vanB genes were detected in 0.9% (1/110) of the samples. The identification of all Staphylococcus species isolated from subclinical mastitis cases and the phenotypic and genotypic resistance characterization in these isolates is of great importance for dairy farming in the state of Piauí, as well as for public health.


Assuntos
Mastite Bovina , Staphylococcus aureus Resistente à Meticilina , Infecções Estafilocócicas , Bovinos , Animais , Feminino , Humanos , Mastite Bovina/microbiologia , Brasil/epidemiologia , Staphylococcus/genética , Infecções Estafilocócicas/epidemiologia , Infecções Estafilocócicas/veterinária , Infecções Estafilocócicas/microbiologia , Testes de Sensibilidade Microbiana , Leite/microbiologia , Antibacterianos/farmacologia
17.
Sci Rep ; 12(1): 15541, 2022 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-36109627

RESUMO

Staphylococcus equorum strain KM1031 is resistant to chloramphenicol, erythromycin and lincomycin. To shed light on the genetic factors underlying these antibiotic resistances, we determined the global gene expression profile of S. equorum KM1031 using RNA sequencing. During chloramphenicol, erythromycin and lincomycin treatment, 8.3% (183/2,336), 16.0% (354/2,336), and 2.9% (63/2,336) of S. equorum KM1031 genes exhibited significant differences in expression, respectively. These three antibiotics upregulated genes related to efflux and downregulated genes related to transporters. Antibiotic treatment also upregulated osmoprotectant-related genes involved in salt tolerance. To identify specific genes functionally related to antibiotic resistance, we compared the genome of strain KM1031 with those of three S. equorum strains that are sensitive to these three antibiotics. We identified three genes of particular interest: an antibiotic biosynthesis monooxygenase gene (abm, AWC34_RS01805) related to chloramphenicol resistance, an antibiotic ABC transporter ATP-binding protein gene (msr, AWC34_RS11115) related to erythromycin resistance, and a lincosamide nucleotydyltransferase gene (lnuA, AWC34_RS13300) related to lincomycin resistance. These genes were upregulated in response to the corresponding antibiotic; in particular, msr was upregulated more than fourfold by erythromycin treatment. Finally, the results of RNA sequencing were validated by quantitative real-time PCR. This transcriptomic analysis provides genetic evidence regarding antibiotic stress responses of S. equorum strain KM1031.


Assuntos
Alimentos Fermentados , Lincomicina , Transportadores de Cassetes de Ligação de ATP/genética , Trifosfato de Adenosina , Antibacterianos/farmacologia , Cloranfenicol/farmacologia , Eritromicina/farmacologia , Lincomicina/farmacologia , Oxigenases de Função Mista , Staphylococcus , Transcriptoma
18.
Comp Immunol Microbiol Infect Dis ; 89: 101870, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36088796

RESUMO

To determine the antibiotypes and frequency of toxin genes in methicillin-resistant Staphylococcus pseudintermedius (MRSP), 281 nasal swab samples were collected from dogs and dog guardians in Abakaliki, Southeastern Nigeria. Antimicrobial susceptibility testing was determined by disc diffusion technique while detection of toxin genes was carried out by PCR. Exactly 41 (28.7 %) and 6 (4.3 %) MRSP were obtained from dogs and dog guardians respectively. Isolates exhibited resistance (100-16.7 %) to amoxicillin-clavulanic acid, cephalosporins, fluoroquinolones, and carbapenems. Seccanine, lukD, siet, and exi toxin genes were harboured by 42 (89.4 %), 47 (100 %), 37 (78.7 %), and 2 (4.3 %) MRSP isolates respectively. This study has shown that dogs and dog guardians in Abakaliki, Southeastern Nigeria are colonized by multiple drug-resistant MRSP which harbour toxin genes. This represents a significant public health problem in veterinary and human medicine.


Assuntos
Anti-Infecciosos , Doenças do Cão , Staphylococcus aureus Resistente à Meticilina , Infecções Estafilocócicas , Combinação Amoxicilina e Clavulanato de Potássio , Animais , Antibacterianos/farmacologia , Anti-Infecciosos/farmacologia , Carbapenêmicos , Cefalosporinas , Doenças do Cão/epidemiologia , Cães , Fluoroquinolonas , Humanos , Resistência a Meticilina/genética , Testes de Sensibilidade Microbiana/veterinária , Nigéria/epidemiologia , Infecções Estafilocócicas/epidemiologia , Infecções Estafilocócicas/veterinária , Staphylococcus
19.
Wei Sheng Yan Jiu ; 51(4): 638-644, 2022 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-36047271

RESUMO

OBJECTIVE: To evaluate the disinfection efficacy and mechanism of neutral electrolyzed water(NEW) on Staphylococcus albus. METHODS: The bactericidal effect of NEW was observed through suspension quantitative bactericidal test. In addition, to analyze the mechanism of NEW, the leakage of bacterial contents and enzyme activity were tested, and the damage of bacterial protein and DNA were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and agarose gel electrophoresis. RESULTS: After treated with NEW for 1 min, staphylococcus albus in suspension(2.07×10~7 CFU/mL)were reduced to below the detection limit(<10 CFU/mL), with killing rate >99.999% and killing log value >5. NEW could destroy the integrity of the cell membrane, resulting in the leakage of Ca~(2+), K~+, protein and nucleic acid, and change the activity of superoxide dismutase(SOD), peroxidase(POD), catalase(CAT) and other enzymes, it could also damage protein and DNA. CONCLUSION: NEW can effectively kill Staphylococcus albus in suspension, and its mechanism involves the destruction of cell membrane, bacterial protein and nucleic acid, and the change of enzyme activity.


Assuntos
Desinfecção , Ácidos Nucleicos , Proteínas de Bactérias , Desinfecção/métodos , Eletrólise/métodos , Staphylococcus , Água
20.
Microbiol Spectr ; 10(5): e0125122, 2022 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-36094193

RESUMO

Chronic rhinosinusitis (CRS) is a common, yet underreported and understudied manifestation of upper respiratory disease in people with cystic fibrosis (CF). Recently developed standard of care guidelines for the management of CF CRS suggest treatment of upper airway disease may ameliorate lower airway disease. We sought to determine whether changes to sinus microbial community diversity and specific taxa known to cause CF lung disease are associated with increased respiratory disease and inflammation. We performed 16S rRNA gene sequencing, supplemented with cytokine analyses, microscopy, and bacterial culturing, on samples from the sinuses of 27 adults with CF CRS. At each study visit, participants underwent endoscopic paranasal sinus sampling and clinical evaluation. We identified key drivers of microbial community composition and evaluated relationships between diversity and taxa with disease outcomes and inflammation. Sinus community diversity was low, and the composition was unstable, with many participants exhibiting alternating dominance between Pseudomonas aeruginosa and staphylococci over time. Despite a tendency for dominance by these two taxa, communities were highly individualized and shifted composition during exacerbation of sinus disease symptoms. Exacerbations were also associated with communities dominated by Staphylococcus spp. Reduced microbial community diversity was linked to worse sinus disease and the inflammatory status of the sinuses (including increased interleukin-1ß [IL-1ß]). Increased IL-1ß was also linked to worse sinus endoscopic appearance, and other cytokines were linked to microbial community dynamics. Our work revealed previously unknown instability of sinus microbial communities and a link between inflammation, lack of microbial community diversity, and worse sinus disease. IMPORTANCE Together with prior sinus microbiota studies of adults with CF chronic rhinosinusitis, our study underscores similarities between sinus and lower respiratory tract microbial community structures in CF. We show how community structure tracks with inflammation and several disease measures. This work strongly suggests that clinical management of CRS could be leveraged to improve overall respiratory health in CF. Our work implicates elevated IL-1ß in reduced microbiota diversity and worse sinus disease in CF CRS, suggesting applications for existing therapies targeting IL-1ß. Finally, the widespread use of highly effective cystic fibrosis transmembrane conductance regulator (CFTR) modulator therapy has led to less frequent availability of spontaneous expectorated sputum for microbiological surveillance of lung infections. A better understanding of CF sinus microbiology could provide a much-needed alternative site for monitoring respiratory infection status by important CF pathogens.


Assuntos
Fibrose Cística , Microbiota , Sinusite , Adulto , Humanos , Fibrose Cística/complicações , Fibrose Cística/tratamento farmacológico , Fibrose Cística/microbiologia , Regulador de Condutância Transmembrana em Fibrose Cística/uso terapêutico , Interleucina-1beta/uso terapêutico , RNA Ribossômico 16S/genética , Sinusite/complicações , Sinusite/diagnóstico , Sinusite/microbiologia , Microbiota/genética , Staphylococcus/genética , Inflamação , Doença Crônica
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...