Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 86.414
Filtrar
1.
Braz. j. biol ; 84: e253508, 2024. tab, ilus
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1360218

RESUMO

Present research work represents antiviral and antibacterial value of body fat of Saara hardwickii commonly called as spiny tailed lizard. Oil was extracted from body fats located in the ventral region of this animal using hydrocarbons e.g., n-hexane, methanol, butanol and ethyl acetate as a solvent. The antibacterial activity of lizard oil was tested against standard as well as multi-resistant lines ofEscherichia coli, Styphalococcus aureus, Pseudomonas aeruginosa and Proteus vulgaris alone and with antibiotic ampicillin. For antibacterial potential, Ethyl acetate and Butanol solvent extract showed best zone of inhibition (7mm) with P. aeruginosa and S. aureus respectively. For antiviral potential, Butanol and Methanol extract showed best HA (Hemagglutination) titer of 04 with NDV and IBV viral strain respectively. It is concluded that lizard oil has antimicrobial potential against different pathogens strains (virus, bacteria).


O presente trabalho de pesquisa apresenta a importância antiviral e antibacteriana da gordura corporal de Saara hardwickii, comumente chamado de lagarto de cauda espinhosa. O óleo foi extraído de gorduras corporais localizadas na região ventral desse animal usando hidrocarbonetos, por exemplo, n-hexano, metanol, butanol e acetato de etila, como solvente. A atividade antibacteriana do óleo do lagarto foi testada em linhagens padrão e multirresistentes de Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa e Proteus vulgaris, de forma isolada e com antibiótico ampicilina. Para o potencial antibacteriano, acetato de etila e extrato de butanol apresentaram melhor zona de inibição (7 mm) com P. aeruginosa e S. aureus, respectivamente. Para o potencial antiviral, o extrato de butanol e o extrato de metanol apresentaram melhor título de hemaglutinação de 4 com as cepas virais NDV e IBV, respectivamente. Conclui-se que o óleo do lagarto possui potencial antimicrobiano contra diferentes cepas de patógenos (vírus e bactérias).


Assuntos
Animais , Antivirais , Tecido Adiposo , Lagartos , Antibacterianos
2.
Rev. bras. med. esporte ; 29(spe1): e2022_0190, 2023. tab
Artigo em Inglês | LILACS | ID: biblio-1394851

RESUMO

ABSTRACT Introduction A healthy body depends on several factors, and physical exercise is one of the main factors. Research results on the physical health of female university students show that although the general health status of Chinese female students is good, the quality of physical performance at university continues to show a slow downward trend. Objective To explore the effects of different exercises on the fat mass and lean weight indices of female university students. Methods 60 female students who participated in aerobics and ball games training in the Physical Education Department of a university were randomly divided into a control group and a training group, with 30 volunteers in each group. The experimental group performed intensive aerobics training and ball training. In contrast, no intervention was performed in the control group, and indicators were collected and analyzed according to current literature. Results After eight weeks of the intervention protocol, the body weight, body fat weight, lean weight, body surface area, and body fat ratio of the control group had no significant changes from the period before the experiment (P > 0.05). In the training group, fat weight decreased significantly (p<0.01), lean mass weight increased significantly (p<0.01), body fat ratio decreased (p<0.01), and body weight and body surface area had no significant changes (p>0.05). PTH and FSH in the training group were significantly higher than in the control group (P < 0.05). As the aerobic exercise level improved in the experimental group, the weight and abdominal skinfold thickness in the exercise group showed a downward trend. Conclusion There is a general correlation between BMI and body fat content and its body distribution in college women. Compared to men, BMI is better suited to describe the fat metabolism of college women. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.


RESUMO Introdução Um corpo saudável depende de vários fatores e o exercício físico é um dos principais. Os resultados de pesquisa na saúde física das estudantes universitárias mostram que apesar do estado de saúde geral das estudantes chinesas serem bons, a qualidade no desempenho físico na universidade continua a mostrar uma lenta tendência de queda. Objetivo Explorar os efeitos de distintos exercícios sobre os índices de gordura e massa magra de universitárias. Métodos 60 alunas que participaram de treinamento de aeróbica e jogos de bola no Departamento de Educação Física de uma universidade foram divididas aleatoriamente em grupo de controle e grupo de treinamento, com 30 voluntárias em cada grupo. O grupo experimental realizou treinamento intensivo de aeróbica e treinamento com bola, enquanto nenhuma intervenção foi executada no grupo controle, a coleta e análise dos indicadores foi executada segundo a literatura corrente. Resultados Após 8 semanas do protocolo de intervenção, o peso corporal, peso da gordura corporal, peso magro, área de superfície corporal e relação de gordura corporal do grupo controle não tiveram alterações significativas em relação ao período anterior ao experimento (P > 0,05). No grupo de treinamento, o peso da gordura diminuiu significativamente (p<0,01), o peso da massa magra aumentou significativamente (p<0,01), a relação de gordura corporal diminuiu (p<0,01), o peso corporal e a superfície corporal não sofreram alterações significativas (p>0,05). O PTH e FSH no grupo de treinamento foram significativamente maiores do que os do grupo controle (P < 0,05). Com a melhora do nível de exercício aeróbico no grupo experimental, o peso e a espessura da dobra cutânea abdominal no grupo de exercício apresentaram uma tendência de queda. Conclusão Há uma correlação geral entre o IMC e o teor de gordura corporal e a sua distribuição corporal em universitárias. Em comparação com os homens, o IMC é mais adequado para descrever o metabolismo de gordura de universitárias. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.


RESUMEN Introducción Un cuerpo sano depende de varios factores y el ejercicio físico es uno de los principales. Los resultados de la investigación sobre la salud física de las estudiantes universitarias muestran que, aunque el estado de salud general de las estudiantes chinas es bueno, la calidad del rendimiento físico en la universidad sigue mostrando una lenta tendencia a la baja. Objetivo Explorar los efectos de diferentes ejercicios sobre los índices de grasa y peso magro de estudiantes universitarias. Métodos 60 alumnas que participaban en el entrenamiento de aeróbic y juegos de pelota en el Departamento de Educación Física de una universidad fueron divididas aleatoriamente en un grupo de control y un grupo de entrenamiento, con 30 voluntarias en cada grupo. El grupo experimental realizó un entrenamiento aeróbico intensivo y un entrenamiento con balón, mientras que en el grupo de control no se llevó a cabo ninguna intervención; la recogida y el análisis de los indicadores se realizaron de acuerdo con la literatura actual. Resultados Tras 8 semanas del protocolo de intervención, el peso corporal, el peso de la grasa corporal, el peso magro, la superficie corporal y la proporción de grasa corporal del grupo de control no presentaron cambios significativos en comparación con el periodo anterior al experimento (P > 0,05). En el grupo de entrenamiento, el peso de la grasa disminuyó significativamente (p<0,01), el peso de la masa magra aumentó significativamente (p<0,01), la proporción de grasa corporal disminuyó (p<0,01), el peso corporal y la superficie corporal no tuvieron cambios significativos (p>0,05). La PTH y la FSH en el grupo de entrenamiento fueron significativamente mayores que las del grupo de control (P < 0,05). Con la mejora del nivel de ejercicio aeróbico en el grupo experimental, el peso y el grosor del pliegue cutáneo abdominal en el grupo de ejercicio mostraron una tendencia a la baja. Conclusión Existe una correlación general entre el IMC y el contenido de grasa corporal y su distribución en las estudiantes universitarias. En comparación con los hombres, el IMC es más adecuado para describir el metabolismo de las grasas de las estudiantes universitarias. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.


Assuntos
Humanos , Feminino , Esportes , Estudantes , Índice de Massa Corporal , Tecido Adiposo , Saúde da Mulher , Estudos de Casos e Controles
3.
J Biomed Mater Res A ; 111(1): 88-105, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36205241

RESUMO

Mesenchymal stem cells have contributed to the continuous progress of tissue engineering and regenerative medicine. Adipose-derived stem cells (ADSC) possess many advantages compared to other origins including easy tissue harvesting, self-renewal potential, and fast population doubling time. As multipotent cells, they can differentiate into osteoblastic cell linages. In vitro bone models are needed to carry out an initial safety assessment in the study of novel bone regeneration therapies. We hypothesized that 3D bone-on-a-chip models containing ADSC could closely recreate the physiological bone microenvironment and promote differentiation. They represent an intermedium step between traditional 2D-in vitro and in vivo experiments facilitating the screening of therapeutic molecules while saving resources. Herein, we have differentiated ADSC for 7 and 14 days and used them to fabricate in vitro bone models by embedding the pre-differentiated cells in a 3D collagen matrix placed in a microfluidic chip. Osteogenic markers such as alkaline phosphatase activity, calcium mineralization, changes on cell morphology, and expression of specific proteins (bone sialoprotein 2, dentin matrix acidic phosphoprotein-1, and osteocalcin) were evaluated to determine cell differentiation potential and evolution. This is the first miniaturized 3D-in vitro bone model created from pre-differentiated ADSC embedded in a hydrogel collagen matrix which could be used for personalized bone tissue engineering.


Assuntos
Hidrogéis , Engenharia Tecidual , Hidrogéis/farmacologia , Hidrogéis/metabolismo , Dispositivos Lab-On-A-Chip , Células Cultivadas , Diferenciação Celular , Osteogênese , Células-Tronco , Tecido Adiposo , Colágeno/metabolismo
4.
J Ethnopharmacol ; 301: 115824, 2023 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-36273747

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Initially recorded in Yifang Jijie (an ancient Chinese text), Qi Gong Wan (QGW) is used to treat obese women with infertility. QGW can help promote follicular development and maturation, regulate the balance of serum hormones between testosterone and estradiol, enhance endometrial receptivity, improve waist circumference, and ameliorate insulin resistance. It contains eight herbs: Pinellia ternata (Thunb.) Makino (Banxia), Citrus maxima (Burm.) (Juhong), Poria cocos (Schw.) Wolf. (Fuling), Atractylodes macrocephala Koidz (Baizhu), Cyperus rotundus L. (Xiangfu), Conioselinum anthriscoides 'Chuanxiong' (Chuanxiong), Massa Medicata Fermentata (Shenqu), and Glycyrrhiza uralensis Fisch. ex DC. (Gancao). However, the underlying mechanism of how QGW affects women with PCOS remains unclear. AIM OF THE STUDY: QGW has been widely used to treat PCOS patients with obesity clinically. This study was designed to identify its chemical and pharmacological properties. MATERIALS AND METHODS: Network pharmacology was used to predict the active compounds, potential targets, and pathways of QGW. Female C57BL/6J mice were injected with letrozole and fed a high-fat diet to establish a PCOS-insulin resistance (PCOS-IR) model. Body weight, estrous cycles, ovarian pathology, and serum insulin resistance were measured. qRT-PCR was used to examine the inflammation-related and steroid hormone biosynthesis-related mRNA expression in adipose tissue. Western blotting was used to determine the protein levels of Nrf2, HO-1, and Cyp1b1 in adipose tissue. Molecular docking was used to reveal the key chemical compounds of QGW. RESULTS: Network pharmacology revealed a total of 91 active ingredients in QGW that were associated with 167 targets. QGW could potentially treat PCOS-IR via nitrogen metabolism, steroid hormone biosynthesis, and ovarian steroidogenesis pathways. In the PCOS-IR mouse model, we found that QGW decreased the mean diameter of adipocytes and the total adipocyte area. Furthermore, QGW was found to significantly lower the expression of inflammation-related genes including Tnfɑ and C4a/b and the steroid hormone biosynthesis-related gene Cyp1b1. QGW showed a tendency to improve cystic follicles, fasting insulin, and HOMA-IR index in the PCOS-IR mouse model. Combining these findings with the results of KEGG analysis, we conclude that QGW promotes the Nrf2/HO-1/Cyp1b1 pathway to protect adipose tissue under conditions of PCOS. Molecular docking revealed that rutin, nicotiflorin, and baicalein may be the key chemical compounds of QGW through which it improves adipocyte hypertrophy and inflammation. CONCLUSIONS: QGW improved adipocyte hypertrophy and inflammation in the PCOS-IR mouse model by activating the Nrf2/HO-1/Cyp1b1 pathway to protect adipose tissue. Our work thus provides a new research avenue for the study of traditional Chinese medicine in the treatment of PCOS.


Assuntos
Resistência à Insulina , Síndrome do Ovário Policístico , Qigong , Animais , Feminino , Humanos , Camundongos , Adipócitos/metabolismo , Tecido Adiposo/metabolismo , Modelos Animais de Doenças , Estradiol , Hipertrofia/patologia , Inflamação/metabolismo , Insulina , Resistência à Insulina/fisiologia , Camundongos Endogâmicos C57BL , Simulação de Acoplamento Molecular , Farmacologia em Rede , Fator 2 Relacionado a NF-E2/metabolismo , Obesidade/metabolismo , Síndrome do Ovário Policístico/tratamento farmacológico
5.
Phys Med Rehabil Clin N Am ; 34(1): 1-23, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36410877

RESUMO

In recent years, autologous biological preparations have emerged as a growing area of medical innovation in interventional orthopedical procedures and surgical interventions. These cellular therapies are often referred to as orthobiologics and are derived from patient's own tissues, such as blood, bone marrow, and adipose tissue to prepare platelet-rich plasma (PRP), bone marrow concentrate, and adipose tissue concentrate, respectively. In this article, we will emphasize and discuss the physiological variability of autologous PRP bioformulations regarding their effectivity in tissue repair. Furthermore, recent developments concerning platelet dosing, potentially effecting immunomodulation, and pain killing will be described.


Assuntos
Transplante de Células-Tronco Hematopoéticas , Procedimentos Ortopédicos , Plasma Rico em Plaquetas , Humanos , Cicatrização , Tecido Adiposo
6.
Phys Med Rehabil Clin N Am ; 34(1): 25-47, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36410885

RESUMO

In recent years, autologous biological preparations have emerged as a growing area of medical innovation in interventional orthopedical procedures and surgical interventions. These cellular therapies are often referred to as orthobiologics and are derived from patient's own tissues, like blood, bone marrow, and adipose tissue to prepare platelet-rich plasma (PRP), bone marrow concentrate (BMC), and adipose tissue concentrate (ATC), respectively. In this article, we emphasize and discuss the physiologic variability of autologous prepared BMC and ATC for the delivery of mesenchymal stem cells to support tissue repair processes.


Assuntos
Transplante de Células-Tronco Hematopoéticas , Células-Tronco Mesenquimais , Plasma Rico em Plaquetas , Humanos , Medula Óssea , Tecido Adiposo
7.
Phys Med Rehabil Clin N Am ; 34(1): 285-290, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36410888

RESUMO

There is a pressing need for the standardization of orthobiologics, considering the cellular components, concentrations, and methods of injections may vary wildly, currently without significant standards of care. There is a growing body of evidence that these factors matter significantly for patient outcomes, so it is imperative that orthobiologic constituents are measured and standardized. Cell counts may be performed for platelet-rich plasma and bone marrow aspirate-based injections, whereas adipose should have standardized processing techniques as cellular quantification is more difficult.


Assuntos
Plasma Rico em Plaquetas , Humanos , Tecido Adiposo , Injeções , Padrões de Referência
8.
Methods Mol Biol ; 2598: 75-85, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36355286

RESUMO

Adipose-derived stromal cells (ASC) are a promising alternative cell source to chondrocytes as well as to bone marrow-derived mesenchymal stromal cells (BMSC) in cartilage tissue engineering and repair. Here we describe ASC isolation from liposuction by-products by collagenase-based tissue digestion combined with cell filtration and followed by monolayer attachment and expansion culture. Quality control requires confirmation of correct surface marker expression and multilineage differentiation potential by a trilineage differentiation assay.


Assuntos
Tecido Adiposo , Condrogênese , Diferenciação Celular , Células Estromais/metabolismo , Cartilagem , Condrócitos , Células Cultivadas , Células da Medula Óssea
9.
Clin Plast Surg ; 50(1): 171-179, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36396256

RESUMO

Refined buttock augmentation with fat grafting in Chinese women during the past decade is introduced. The ideal buttock contouring outcome and figure silhouette come from the proper individualized plan and meticulous maneuvers through fat grafting to the buttocks combined with liposuction procedures on the surrounding areas of the buttocks as well as on the other body parts. The fat grafts are collected, filtered, and condensed by gravitation in a sterilized canister during liposuction. It is recommended that fat grafts were only transplanted into the subcutaneous layers and with no injection into the musculatures of the buttocks. High patients' satisfaction was obtained with no major complications and fewer minor complications.


Assuntos
Tecido Adiposo , Lipectomia , Humanos , Feminino , Nádegas/cirurgia , Tecido Adiposo/transplante , Lipectomia/métodos , Transplante Autólogo , Satisfação do Paciente
10.
Clin Plast Surg ; 50(1): 19-31, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36396258

RESUMO

Autologous fat grafting and facial liposuction are widely used to reshape facial contours. But up to now, there is no surgical design idea for Asian women. The article aimed to provide a facial esthetic design to guide the combined operation of facial liposuction and fat grafting for Asians, that is, the "three-line and nine-point" design method. In retrospective reviews, the surgical results of clinical practice were satisfactory, and a more attractive facial appearance was obtained. Our technique can easily be learnt and can be mastered by the most well-qualified plastic surgeons for satisfactory outcomes.


Assuntos
Lipectomia , Feminino , Humanos , Estudos Retrospectivos , Tecido Adiposo/transplante , Face/cirurgia , Asiáticos
11.
Food Chem ; 403: 134477, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36358090

RESUMO

The effects of theasinensin A (TSA) on healthy C57BL/6J mice were investigated. Results showed that long-term administration of 100 mg/kg body weight/day TSA might be non-toxic to healthy mice based on the unaltered basal biochemical indicators related to glucose and lipid metabolism, inflammatory factors and hepatic injury. On the contrary, TSA stimulated the rate of lipid turnover and browning of white adipose tissues, accelerated the adipocytic energy mobilization, and then reduced the white adipocytic size, ultimately enhancing resistance to obesity in healthy mice. Furthermore, TSA not only up-regulated the expression of mucin, tight junction protein, and short-chain fatty acids receptor, but also regulated the intestinal microbiota by enhancing the typical beneficial microbe Akkermansia muciniphila, thereby modulating the colonic microenvironment. These results suggested that TSA had a potential strengthening effect on the resistance of healthy mice to metabolic disorders, which provides a theoretical basis for the utilization of TSA.


Assuntos
Benzopiranos , Fenóis , Camundongos , Animais , Camundongos Endogâmicos C57BL , Fenóis/metabolismo , Tecido Adiposo Branco , Dieta Hiperlipídica , Tecido Adiposo/metabolismo
12.
Environ Res ; 216(Pt 3): 114685, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36341787

RESUMO

Obesity is a major threat to health, but the etiology of obesity is incompletely understood. Phthalates, synthetic chemicals ubiquitous in the environment, are suspected to have obesogenic effects, but the relationship of phthalates and obesity in humans remains uncertain. We examined whether phthalate exposure was associated with body fat gain in midlife women. We analyzed data from 1369 women in the Study of Women's Health Across the Nation Multi-Pollutant Study. Eleven phthalate metabolites measured in spot urine samples at baseline (1999/2000) were standardized with covariate-adjusted creatinine. Body weight (BW), fat mass (FM) from dual-energy X-ray absorptiometry (DXA), and body fat percentage (BF%) from DXA were measured near-annually until 2016/2017. For each metabolite, linear mixed effects models with time and log2(metabolite) interactions were examined, adjusting for demographic, lifestyle, and menopause-related factors. Analyses were conducted overall and stratified by baseline obesity status. As sensitivity analyses, all analyses were repeated using a second set of metabolites measured in 2002/2003. Higher levels of all metabolites except mono-carboxy-isononyl phthalate were associated with faster increases in BF%. Per doubling of metabolite concentrations, differences in five-year BF% change ranged from 0.03 percentage point (ppt) (95% confidence interval (CI): -0.03, 0.09) for mono-isobutyl phthalate to 0.09 ppt (95% CI: 0.02, 0.16) for mono(3-carboxypropyl) phthalate. Results were similar for FM change, but associations with BW change were mostly null. In stratified analyses by baseline obesity status, positive associations were strongest in women who were normal/underweight at baseline. When metabolites from 2002/2003 were used as exposures, most associations were attenuated and not statistically significant, but they remained positive for normal/underweight women. In conclusion, phthalate metabolites were associated with more rapid body fat gain in midlife women, but our results need confirmation given attenuation of estimates in the sensitivity analyses.


Assuntos
Poluentes Ambientais , Ácidos Ftálicos , Feminino , Humanos , Poluentes Ambientais/urina , Magreza , Exposição Ambiental/análise , Ácidos Ftálicos/urina , Obesidade/induzido quimicamente , Obesidade/epidemiologia , Tecido Adiposo/metabolismo , Saúde da Mulher
13.
J Nutr Biochem ; 111: 109153, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36150680

RESUMO

This study aimed to characterize the potential beneficial effects of chronic docosahexaenoic acid (DHA) supplementation on restoring subcutaneous white adipose tissue (scWAT) plasticity in obese aged female mice. Two-month-old female C57BL/6J mice received a control (CT) or a high fat diet (HFD) for 4 months. Then, 6-month-old diet-induced obese (DIO) mice were distributed into the DIO and the DIOMEG group (fed with a DHA-enriched HFD) up to 18 months. In scWAT, the DHA-enriched diet reduced the mean adipocyte size and reversed the upregulation of lipogenic genes induced by the HFD, reaching values even lower than those observed in CT animals. DIO mice exhibited an up-regulation of lipolytic and fatty oxidation gene expressions that was reversed in DHA-supplemented mice except for Cpt1a mRNA levels, which were higher in DIOMEG as compared to CT mice. DHA restored the increase of proinflammatory genes observed in scWAT of DIO mice. While no changes were observed in total macrophage F4/80+/CD11b+ content, the DHA treatment switched scWAT macrophages profile by reducing the M1 marker Cd11c and increasing the M2 marker CD206. These events occurred alongside with a stimulation of beige adipocyte specific genes, the restoration of UCP1 and pAKT/AKT ratio, and a recovery of the HFD-induced Fgf21 upregulation. In summary, DHA supplementation induced a metabolic remodeling of scWAT to a healthier phenotype in aged obese mice by modulating genes controlling lipid accumulation in adipocytes, reducing the inflammatory status, and inducing beige adipocyte markers in obese aged mice.


Assuntos
Ácidos Docosa-Hexaenoicos , Obesidade , Feminino , Camundongos , Animais , Camundongos Obesos , Ácidos Docosa-Hexaenoicos/farmacologia , Ácidos Docosa-Hexaenoicos/metabolismo , Obesidade/metabolismo , Camundongos Endogâmicos C57BL , Tecido Adiposo Branco/metabolismo , Dieta Hiperlipídica/efeitos adversos , Gordura Subcutânea/metabolismo , Suplementos Nutricionais , Tecido Adiposo/metabolismo
14.
Cytokine ; 161: 156080, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36368230

RESUMO

OBJECTIVE: Interleukin-33 (IL-33) is associated with obesity-related inflammation. We aim to investigate IL-33 expression in subcutaneous adipose tissue (SAT) in type 2 diabetes (T2D) subjects and its effects on human adipocyte glucose uptake. METHODS: Expression of IL-33 was analysed in SAT from cohort studies including subjects with and without obesity and T2D and correlated with insulin resistance and obesity markers. Magnetic resonance imaging (MRI) of tissue fat volumes was performed. We investigated the effects of IL-33 treatment on ex vivo adipocyte glucose uptake. RESULTS: T2D subjects had higher IL-33 gene and protein expression in SAT than the control subjects. IL-33 mRNA expression was positively correlated with markers of dysglycemia (e.g. HbA1c), insulin resistance (e.g. HOMA-IR) and adiposity (BMI, visceral adipose tissue volume, liver and pancreas fat %). In multiple linear regression analyses, insulin resistance and T2D status were the strongest predictors of IL-33, independent of BMI. IL-33 mRNA expression was negatively correlated with expression of genes regulating adipocyte glucose uptake, lipid storage, and adipogenesis (e.g.glucose transporter 1 and 4 (GLUT1/4), fatty acid binding protein 4 (FABP4), and PPARG). Additionally, incubation of SAT with IL-33 reduced adipocyte glucose uptake and GLUT4 gene and protein expression. CONCLUSIONS: Our findings suggest that T2D subjects have higher IL-33 gene and protein expressionin SATthan control subjects, which is associated with insulin resistance and reduced gene expression of lipid storage and adipogenesis markers. IL-33 may reduce adipocyte glucose uptake. This opens up a potential pharmacological route for reversing insulin resistance in T2D and prediabetes.


Assuntos
Diabetes Mellitus Tipo 2 , Resistência à Insulina , Humanos , Diabetes Mellitus Tipo 2/metabolismo , Interleucina-33/metabolismo , Adipócitos/metabolismo , Tecido Adiposo/metabolismo , Obesidade/metabolismo , Glucose/metabolismo , RNA Mensageiro/metabolismo , Lipídeos
15.
Ann Anat ; 245: 151995, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36182003

RESUMO

INTRODUCTION: The knee is likely to get hurt due to its excessive weight-bearing, for which it is surrounded by strongly tensioned ligaments, connectives and muscles. These highly active structures are imbedded in fatty tissue. The Fatty and loose connective tissue of the knee recently gained a renaissance in research. While the Hoffa fat body in the ventral knee attracted attention over the last years, we have investigated a smaller, dorsal fat body, ventral to the popliteus muscle. This fat body has not been described before. MATERIALS AND METHODS: 11 knees of 11 fresh specimens were investigated. All muscles but the popliteus muscle were removed. The popliteus was released from its tibial origin and dissected towards its tendinous insertion. Thereby, a subpopliteal fat body (SFB) was shown. The related vessels and nerves were evaluated. The size of the body was measured. Examples of histological slices were stained with HE and immunostained against neurofilament. RESULTS: The SFB lies ventral of the popliteus muscle at the concave posterior tibia and attaches to the periosteum and the popliteus muscle. It is not attached to the posterior cruciate ligament. It is separated from the subpopliteal recess by a lamella deriving from the fibular head. Arterial and venous vessels are seen entering the SFB, deriving from the popliteal artery or the anterior tibial artery. A subbranch of the tibial nerve was seen to reach the SFB. The SFB could be identified in MRI scans and in plastinations. DISCUSSION: Primarily, the SFB provides a gliding space for the mobile part of the popliteus muscle over the tibia. The SFB lies within the tibial concavity, ventral to the popliteus muscle. This is exactly where embryologically, the popliteal artery passes through, before its involution in later stages. Therefore, the SFB may show the former perivascular autonomic nerves which encompass the embryologically created arteries, from which we have seen the arterial remnants. The nerves seen here form neurovascular bundles which could be a source of pain, when compressed. This anatomy may explain the autonomic component of pain in the deep lateral region of the knee. The SFB is functional fat, comparable to the Hoffa's fat pad in the ventral knee.


Assuntos
Corpo Adiposo , Ligamento Cruzado Posterior , Animais , Humanos , Articulação do Joelho/anatomia & histologia , Tecido Adiposo , Dor
16.
J Nutr Biochem ; 111: 109175, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36223834

RESUMO

Increased body iron stores and inflammation in adipose tissue have been implicated in the pathogenesis of insulin resistance (IR) and type 2 diabetes mellitus. However, the underlying basis of these associations is unclear. To attempt to investigate this, we studied the development of IR and associated inflammation in adipose tissue in the presence of increased body iron stores. Male hepcidin knock-out (Hamp1-/-) mice, which have increased body iron stores, and wild-type (WT) mice were fed a high-fat diet (HFD) for 12 and 24 weeks. Development of IR and metabolic parameters linked to this, insulin signaling in various tissues, and inflammation and iron-related parameters in visceral adipose tissue were studied in these animals. HFD-feeding resulted in impaired glucose tolerance in both genotypes of mice. In response to the HFD for 24 weeks, Hamp1-/- mice gained less body weight and developed less systemic IR than corresponding WT mice. This was associated with less lipid accumulation in the liver and decreased inflammation and lipolysis in the adipose tissue in the knock-out mice, than in the WT animals. Fewer macrophages infiltrated the adipose tissue in the knockout mice than in wild-type mice, with these macrophages exhibiting a predominantly anti-inflammatory (M2-like) phenotype and indirect evidence of a possible lowered intracellular iron content. The absence of hepcidin was thus associated with attenuated inflammation in the adipose tissue and increased whole-body insulin sensitivity, suggesting a role for it in these processes.


Assuntos
Diabetes Mellitus Tipo 2 , Resistência à Insulina , Masculino , Camundongos , Animais , Resistência à Insulina/fisiologia , Dieta Hiperlipídica/efeitos adversos , Hepcidinas/metabolismo , Diabetes Mellitus Tipo 2/metabolismo , Camundongos Endogâmicos C57BL , Tecido Adiposo/metabolismo , Inflamação/metabolismo , Insulina/metabolismo , Camundongos Knockout , Ferro/metabolismo
17.
J Nutr Biochem ; 111: 109173, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36228975

RESUMO

The antidiabetic effects of green tea have been demonstrated in clinical trials and epidemiological studies. This study investigated the antidiabetic effects of green tea extract (GTE) and its underlying molecular mechanisms using a leptin receptor-deficient db/db mouse model (Leprdb/db). Treatment with GTE for 2 weeks improved glucose tolerance and insulin sensitivity in Leprdb/db mice. In addition, GTE treatment reduced the body weight and adiposity of Leprdb/db mice. Furthermore, GTE treatment reduced pro-inflammatory gene expression, including nuclear factor kappa B (NF-κB) in white adipose tissue (WAT), and also reduced dipeptidyl peptidase-4 (DPP4) expression levels in WAT as well as in the serum. The promoter region of Dpp4 contains the NF-κB binding site, and DPP4 was found to be a direct target of NF-κB. Consistently, in vitro treatment of cells with GTE or its main constituent epigallocatechin gallate reduced lipopolysaccharide-induced NF-κB/DPP4 expression in 3T3-L1 adipocytes and RAW264.7 cells. Overall, our data demonstrated that GTE exerts an anti-diabetic effect by regulating the expression levels of NF-κB and DPP4 in WAT.


Assuntos
Dipeptidil Peptidase 4 , Hipoglicemiantes , Camundongos , Animais , Hipoglicemiantes/farmacologia , Hipoglicemiantes/uso terapêutico , Hipoglicemiantes/metabolismo , Dipeptidil Peptidase 4/genética , Dipeptidil Peptidase 4/metabolismo , NF-kappa B/metabolismo , Extratos Vegetais/uso terapêutico , Tecido Adiposo/metabolismo , Chá/química
18.
Clin Imaging ; 93: 4-11, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36335677

RESUMO

PURPOSE: Myosteatosis, which is associated with a variety of cardiometabolic illnesses, represents muscle quality, an important aspect of sarcopenia. A new laboratory marker for myosteatosis has been required to more readily identify it. We investigated whether serum gamma-glutamyl transferase (GGT) levels are associated with myosteatosis measured by computed tomography (CT). METHODS: The total abdominal muscle area (TAMA) of 13,452 subjects was measured at the L3 level with abdominal CT. TAMA was segmented into intramuscular adipose tissue and skeletal muscle area (SMA), which was further classified into normal attenuation muscle area (NAMA) and low attenuation muscle area (LAMA). The following variables were adopted as indicators of myosteatosis: SMA/body mass index (BMI), NAMA/BMI, LAMA/BMI, and NAMA/TAMA. Logistic regression analysis was used to examine the odds ratio (OR) of each GGT quartile for the highest quartile of myosteatosis indices in each sex. RESULTS: The mean age and serum GGT levels were 53.7 years and 32.8 IU/L (standard deviation [SD], 37.6), respectively, in men, and 53.2 years and 18.4 IU/L (SD, 19.8) in women. In both sexes, the ORs of all myosteatosis indices differed significantly between GGT quartiles. Indices of good- and poor-quality muscle were negatively and positively correlated with GGT levels, respectively. CONCLUSION: Higher GGT levels were significantly associated with advanced myosteatosis defined by reliable CT indices. This result opens the possibility for using GGT as a cost-effective indicator of myosteatosis. Further prospective research on changes to GGT levels with myosteatosis alleviation will validate GGT as a monitoring marker.


Assuntos
Sarcopenia , gama-Glutamiltransferase , Masculino , Humanos , Feminino , Sarcopenia/patologia , Músculo Esquelético/diagnóstico por imagem , Músculo Esquelético/patologia , Tecido Adiposo , Tomografia Computadorizada por Raios X/métodos
19.
Methods Mol Biol ; 2588: 407-415, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36418700

RESUMO

Growing adipose-derived stem cells (ADSC) in serum-free conditions is important as it represents a way of expanding multipotent cells in a clinical grade medium. Most cultured ADSC are expanded and tested in serum-containing media, which can pose significant health risks if these cells were used in clinical applications. Moreover, cells grown in serum-free conditions behave very different than those cultured in serum-containing media. Here, we present a technique to culture adipose-derived stem cells in serum-free conditions. The methods described in this chapter were optimized for ovine ADSC. The appropriate optimization should be done for other cell lines.


Assuntos
Adipócitos , Tecido Adiposo , Animais , Ovinos , Células-Tronco , Testes Imunológicos , Células-Tronco Multipotentes
20.
Pediatr Int ; 64(1): e15323, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36331224

RESUMO

BACKGROUND: Patients with nephrotic syndrome (NS) are at a high risk of cardiovascular disease, obesity, and dyslipidemia. The aim of this study was to evaluate the formation of epicardial adipose tissue (EAT) and investigate electrocardiographic (ECG) parameters in patients. METHODS: Thirty-two patients aged 0-18 years and 15 control patients were compared. In the patient group, physical examination and laboratory parameters were recorded. Atrial depolarization and ventricular repolarization parameters in ECG were compared between the groups. EAT was evaluated with M-mode measurements on echocardiography. RESULTS: There was no difference between the groups in terms of sex, age, body mass index, systolic and diastolic BP. EAT was found to be significantly higher in the patient group. In ECG evaluations it was determined that atrial depolarization and ventricular repolarization parameters increased in the patient group. CONCLUSIONS: Cardiovascular morbidity and mortality are high in kidney diseases. Measurement and follow-up of EAT and ECG findings as a noninvasive parameter can provide information in NS.


Assuntos
Síndrome Nefrótica , Humanos , Síndrome Nefrótica/complicações , Síndrome Nefrótica/diagnóstico , Pericárdio/diagnóstico por imagem , Tecido Adiposo/diagnóstico por imagem , Ecocardiografia , Arritmias Cardíacas
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...