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1.
Transpl Int ; 37: 12310, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38317690

RESUMO

Since the early days of clinical lung transplantation the preservation of donor organs has become a fairly standardized procedure and most centers do follow similar processes. This includes the use of low-potassium high dextran flush solutions and static cold storage (SCS) in a cooler filled with ice. Depending on the length of SCS, organs usually arrive at the recipient hospital at a temperature of 0°C-4°C. The question of the optimal storage temperature for donor lung preservation has been revisited as data from large animal experiments demonstrated that organs stored at 10°C experience less mitochondrial damage. Thus, prolonged cold ischemic times can be better tolerated at 10°C-even in pre-damaged organs. The clinical applicability of these findings was demonstrated in an international multi-center observational study including three high-volume lung transplant centers. Total clinical preservation times of up to 24 hrs have been successfully achieved in organs stored at 10°C without hampering primary organ function and short-term outcomes. Currently, a randomized-controlled trial (RCT) is recruiting patients with the aim to compare standard SCS on ice with prolonged SCS protocol at 10°C. If, as anticipated, this RCT confirms data from previous studies, lung transplantation could indeed become a semi-elective procedure.


Assuntos
Transplante de Pulmão , Preservação de Órgãos , Animais , Humanos , Temperatura Baixa , Gelo , Pulmão , Transplante de Pulmão/métodos , Estudos Observacionais como Assunto , Preservação de Órgãos/métodos , Perfusão/métodos , Ensaios Clínicos Controlados Aleatórios como Assunto , Temperatura , Estudos Multicêntricos como Assunto
2.
PLoS One ; 19(2): e0297067, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38300918

RESUMO

The purpose of this study was to reproduce the previously observed spatial summation of pain effect (SSp) using non-laboratory procedures and commercial equipment. An additional aim was to explore the association between expectations and SSp. The Cold Pressor Task (CPT) was used to induce SSp. Healthy participants (N = 68) immersed their non-dominant hands (divided into 5 segments) into cold water (CPT). Two conditions were used 1) gradual hand immersion (ascending condition) and 2) gradual hand withdrawal (descending condition). Pain intensity was measured on a Visual Analogue Scale (VAS). Psychological factors, such as the participants' expectations of pain intensity were also measured on a VAS. Results showed significant SSp (χ2(4) = 116.90, p < 0.001), reproduced with non-laboratory equipment in a home-based set-up. Furthermore, two novel findings were observed: i) there was a significant correlation between expectations and perceived pain, indicating a link between pain expectations and SSp, ii) spatial summation increased with the increase in duration exposure to the noxious stimulus (Wald χ2(8) = 80.80, p < 0.001). This study suggests that SSp is associated with pain expectations and can be formed by a mixture of excitatory and inhibitory mechanisms potentially driven by temporal characteristics of neural excitation. Moreover, this study proposes a new feasible way to induce SSp using a home-based set-up.


Assuntos
Motivação , Dor , Humanos , Dor/psicologia , Medição da Dor/métodos , Limiar da Dor , Temperatura Baixa
3.
BMC Public Health ; 24(1): 341, 2024 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-38302889

RESUMO

BACKGROUND: Although studies have indicated that extreme temperature is strongly associated with respiratory diseases, there is a dearth of studies focused on children, especially in China. We aimed to explore the association between extreme temperature and children's outpatient visits for respiratory diseases and seasonal modification effects in Harbin, China. METHODS: A distributed lag nonlinear model (DLNM) was used to explore the effect of extreme temperature on daily outpatient visits for respiratory diseases among children, as well as lag effects and seasonal modification effects. RESULTS: Extremely low temperatures were defined as the 1st percentile and 2.5th percentile of temperature. Extremely high temperatures were defined as the 97.5th percentile and 99th percentile of temperature. At extremely high temperatures, both 26 °C (97.5th) and 27 °C (99th) showed adverse effects at lag 0-6 days, with relative risks (RRs) of 1.34 [95% confidence interval (CI): 1.21-1.48] and 1.38 (95% CI: 1.24-1.53), respectively. However, at extremely low temperatures, both - 26 °C (1st) and - 23 °C (2.5th) showed protective effects on children's outpatient visits for respiratory diseases at lag 0-10 days, with RRs of 0.86 (95% CI: 0.76-0.97) and 0.85 (95% CI: 0.75-0.95), respectively. We also found seasonal modification effects, with the association being stronger in the warm season than in the cold season at extremely high temperatures. CONCLUSIONS: Our study indicated that extremely hot temperatures increase the risk of children's outpatient visits for respiratory diseases. Efforts to reduce the exposure of children to extremely high temperatures could potentially alleviate the burden of pediatric respiratory diseases, especially during the warm season.


Assuntos
Transtornos Respiratórios , Doenças Respiratórias , Criança , Humanos , Temperatura , Pacientes Ambulatoriais , Transtornos Respiratórios/epidemiologia , Transtornos Respiratórios/terapia , Doenças Respiratórias/epidemiologia , Doenças Respiratórias/terapia , Temperatura Baixa , Temperatura Alta , China/epidemiologia
4.
Sci Rep ; 14(1): 3181, 2024 02 07.
Artigo em Inglês | MEDLINE | ID: mdl-38326605

RESUMO

While low winter temperatures are associated with increased mortality, this phenomenon has been suggested to be most severe in regions with seemingly mild winters. The study aimed to establish a temperature-based formula that could elucidate the previously ambiguous regional differences in vulnerability to low temperature. European weekly mortality data (2000-2019) were matched with meteorological data to determine for each region vulnerability to temperature decrease and the optimal temperature with lowest mortality. Regression models were developed to generalize and explain these findings considering regional temperature characteristics. Optimal temperature could be predicted based on local average summer temperature (R2 = 85.6%). Regional vulnerability to temperature decrease could be explained by combination of winter and summer temperatures (R2 = 86.1%). Regions with warm winters and cold summers showed the highest vulnerability to decrease of temperature during winter. Contrary to theories about economic disparities Eastern Europe exhibited resistance comparable to Scandinavia. The southern edges of Europe demonstrated serious low temperature vulnerability to decreased temperatures, even if temperature was relatively high around 20 °C. This suggests that the observed connection primarily reflects the modulation of the length of respiratory virus infection seasons by climate conditions, counterbalanced by varying levels of acquired immunity and the presence of heatwaves eliminating the most frail individuals. Thus, relatively low vulnerability and a flat mortality cycle in countries with harsh climates paradoxically imply the presence of threats throughout the whole year.


Assuntos
Clima , Temperatura Baixa , Humanos , Estações do Ano , Temperatura , Europa (Continente)/epidemiologia , Mortalidade , Temperatura Alta
5.
Water Sci Technol ; 89(3): 603-612, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38358492

RESUMO

Nitrous oxide (N2O) is an ozone-depleting greenhouse gas that contributes significantly to the carbon footprint of a wastewater treatment plant (WWTP). Plant-specific measurement campaigns are required to reliably quantify the emission level that has been found to significantly vary between WWTPs. In this study, the N2O emissions were quantified from five full-scale WWTPs during 4-19-day measurement campaigns conducted under both cold period conditions (water temperature below 12 °C) and warm period conditions (water temperature from 12 to 20 °C). The measurement data were studied alongside long-term monitoring data from a sixth WWTP. The calculated emission factors (EFs) varied from near 0 to 1.8% relative to the influent total nitrogen load. The results confirmed a significant seasonality of N2O emissions as well as a notable variation between WWTPs in the emission level, which a single fixed EF cannot represent. Wastewater temperature was one explanatory factor for the emission seasonality. Both low and high emissions were measured from denitrifying-nitrifying activated sludge (AS) processes, while the emissions from only nitrifying AS processes were consistently high. Nitrite (NO2-) at the end of the aerobic zones of the AS process was linked to the variability in N2O emissions during the cold period.


Assuntos
Gases de Efeito Estufa , Óxido Nitroso , Pegada de Carbono , Temperatura Baixa , Esgotos , Água
6.
Phys Rev E ; 109(1-1): 014118, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-38366534

RESUMO

We study the nature of phase transitions in a self-gravitating classical gas in the presence of a central body. The central body can mimic a black hole at the center of a galaxy or a rocky core (protoplanet) in the context of planetary formation. In the chemotaxis of bacterial populations, sharing formal analogies with self-gravitating systems, the central body can be a supply of "food" that attracts the bacteria (chemoattractant). We consider both microcanonical (fixed energy) and canonical (fixed temperature) descriptions and study the inequivalence of statistical ensembles. At high energies (respectively, high temperatures), the system is in a "gaseous" phase and at low energies (respectively, low temperatures) it is in a condensed phase with a "cusp-halo" structure, where the cusp corresponds to the rapid increase of the density of the gas at the contact with the central body. For a fixed density ρ_{*} of the central body, we show the existence of two critical points in the phase diagram, one in each ensemble, depending on the core radius R_{*}: for small radii R_{*}R_{*}^{CCP}, there is no phase transition at all. We study how the nature of these phase transitions changes as a function of the dimension of space. We also discuss the analogies and the differences with phase transitions in the self-gravitating Fermi gas [P. H. Chavanis, Phys. Rev. E 65, 056123 (2002)1063-651X10.1103/PhysRevE.65.056123].


Assuntos
Bactérias , Fatores Quimiotáticos , Quimiotaxia , Temperatura Baixa , Gases
7.
Nature ; 626(7998): 319-326, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38326596

RESUMO

Late Pleistocene ice-age climates are routinely characterized as having imposed moisture stress on low- to mid-latitude ecosystems1-5. This idea is largely based on fossil pollen evidence for widespread, low-biomass glacial vegetation, interpreted as indicating climatic dryness6. However, woody plant growth is inhibited under low atmospheric CO2 (refs. 7,8), so understanding glacial environments requires the development of new palaeoclimate indicators that are independent of vegetation9. Here we show that, contrary to expectations, during the past 350 kyr, peaks in southern Australian climatic moisture availability were largely confined to glacial periods, including the Last Glacial Maximum, whereas warm interglacials were relatively dry. By measuring the timing of speleothem growth in the Southern Hemisphere subtropics, which today has a predominantly negative annual moisture balance, we developed a record of climatic moisture availability that is independent of vegetation and extends through multiple glacial-interglacial cycles. Our results demonstrate that a cool-moist response is consistent across the austral subtropics and, in part, may result from reduced evaporation under cool glacial temperatures. Insofar as cold glacial environments in the Southern Hemisphere subtropics have been portrayed as uniformly arid3,10,11, our findings suggest that their characterization as evolutionary or physiological obstacles to movement and expansion of animal, plant and, potentially, human populations10 should be reconsidered.


Assuntos
Ecossistema , Umidade , Camada de Gelo , Animais , Humanos , Migração Animal , Austrália , Temperatura Baixa , Clima Desértico , História Antiga , Plantas , Pólen , Volatilização
8.
Int J Mol Sci ; 25(3)2024 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-38339044

RESUMO

Spexin (SPX) is a novel adipokine that plays an emerging role in metabolic diseases due to its involvement in carbohydrate homeostasis, weight loss, appetite control, and gastrointestinal movement, among others. In obese patients, SPX plasma levels are reduced. Little is known about the relationship between SPX and white adipose tissue (WAT) thermogenesis. Therefore, the aim of the present study was to evaluate the role of SPX in this process. C57BL/6J male mice were treated or not with SPX for ten days. On day 3, mice were randomly divided into two groups: one kept at room temperature and the other kept at cold temperature (4 °C). Caloric intake and body weight were recorded daily. At the end of the protocol, plasma, abdominal (epididymal), subcutaneous (inguinal), and brown AT (EAT, IAT, and BAT, respectively) depots were collected for measurements. We found that SPX treatment reduced Uncoupling protein 1 levels in WAT under both basal and cold conditions. SPX also reduced cox8b and pgc1α mRNA levels and mitochondrial DNA, principally in IAT. SPX did not modulate the number of beige precursors. SPX decreased spx levels in IAT depots and galr2 in WAT depots. No differences were observed in the BAT depots. In conclusion, we showed, for the first time, that SPX treatment in vivo reduced the thermogenic process in subcutaneous and abdominal AT, being more evident under cold stimulation.


Assuntos
Tecido Adiposo Marrom , Temperatura Baixa , Hormônios Peptídicos , Termogênese , Animais , Humanos , Masculino , Camundongos , Tecido Adiposo Marrom/efeitos dos fármacos , Tecido Adiposo Marrom/fisiologia , Tecido Adiposo Branco/metabolismo , Camundongos Endogâmicos C57BL , Termogênese/efeitos dos fármacos , Termogênese/fisiologia , Proteína Desacopladora 1/metabolismo , Hormônios Peptídicos/farmacologia , Hormônios Peptídicos/fisiologia
9.
Sci Rep ; 14(1): 3266, 2024 02 13.
Artigo em Inglês | MEDLINE | ID: mdl-38351140

RESUMO

Climate change is exacerbating the need for urban greening and the associated environmental and human well-being benefits. Trees can help mitigate urban heat, but more detailed understanding of cooling effects of green infrastructure are needed to guide management decisions and deploy trees as effective and equitable climate adaptation infrastructure. We investigated how urban trees affect summer air temperature along sidewalks within a neighborhood of Tacoma, Washington, USA, and to what extent urban trees reduce risks of high summer temperatures (i.e., the levels regulated by state outdoor heat exposure rules intended to reduce heat-related illnesses). Air temperature varied by 2.57 °C, on average, across our study area, and the probability of daytime temperatures exceeding regulated high temperature thresholds was up to five times greater in locations with no canopy cover within 10 m compared to those with 100% cover. Air temperatures decreased linearly with increasing cover within 10 m, suggesting that every unit of added tree cover can help cool the air. Our findings highlight the value of trees in mitigating urban heat, especially given expected warming with climate change. Protecting existing urban trees and increasing tree cover (e.g., by planting street trees), are important actions to enhance climate change resilience of urban areas.


Assuntos
Temperatura Baixa , Temperatura Alta , Humanos , Temperatura , Estações do Ano , Mudança Climática , Cidades
10.
Lancet Planet Health ; 8(2): e86-e94, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38331534

RESUMO

BACKGROUND: Climate change can directly impact temperature-related excess deaths and might subsequently change the seasonal variation in mortality. In this study, we aimed to provide a systematic and comprehensive assessment of potential future changes in the seasonal variation, or seasonality, of mortality across different climate zones. METHODS: In this modelling study, we collected daily time series of mean temperature and mortality (all causes or non-external causes only) via the Multi-Country Multi-City Collaborative (MCC) Research Network. These data were collected during overlapping periods, spanning from Jan 1, 1969 to Dec 31, 2020. We projected daily mortality from Jan 1, 2000 to Dec 31, 2099, under four climate change scenarios corresponding to increasing emissions (Shared Socioeconomic Pathways [SSP] scenarios SSP1-2.6, SSP2-4.5, SSP3-7.0, and SSP5-8.5). We compared the seasonality in projected mortality between decades by its shape, timings (the day-of-year) of minimum (trough) and maximum (peak) mortality, and sizes (peak-to-trough ratio and attributable fraction). Attributable fraction was used to measure the burden of seasonality of mortality. The results were summarised by climate zones. FINDINGS: The MCC dataset included 126 809 537 deaths from 707 locations within 43 countries or areas. After excluding the only two polar locations (both high-altitude locations in Peru) from climatic zone assessments, we analysed 126 766 164 deaths in 705 locations aggregated in four climate zones (tropical, arid, temperate, and continental). From the 2000s to the 2090s, our projections showed an increase in mortality during the warm seasons and a decrease in mortality during the cold seasons, albeit with mortality remaining high during the cold seasons, under all four SSP scenarios in the arid, temperate, and continental zones. The magnitude of this changing pattern was more pronounced under the high-emission scenarios (SSP3-7.0 and SSP5-8.5), substantially altering the shape of seasonality of mortality and, under the highest emission scenario (SSP5-8.5), shifting the mortality peak from cold seasons to warm seasons in arid, temperate, and continental zones, and increasing the size of seasonality in all zones except the arid zone by the end of the century. In the 2090s compared with the 2000s, the change in peak-to-trough ratio (relative scale) ranged from 0·96 to 1·11, and the change in attributable fraction ranged from 0·002% to 0·06% under the SSP5-8.5 (highest emission) scenario. INTERPRETATION: A warming climate can substantially change the seasonality of mortality in the future. Our projections suggest that health-care systems should consider preparing for a potentially increased demand during warm seasons and sustained high demand during cold seasons, particularly in regions characterised by arid, temperate, and continental climates. FUNDING: The Environment Research and Technology Development Fund of the Environmental Restoration and Conservation Agency, provided by the Ministry of the Environment of Japan.


Assuntos
Mudança Climática , Temperatura Baixa , Temperatura , Estações do Ano , Estudos Prospectivos
11.
BMC Plant Biol ; 24(1): 101, 2024 Feb 09.
Artigo em Inglês | MEDLINE | ID: mdl-38331759

RESUMO

BACKGROUND: The cultivation of bananas encounters substantial obstacles, particularly due to the detrimental effects of cold stress on their growth and productivity. A potential remedy that has gained attention is the utilization of ethyl mesylate (EMS)-induced mutagenesis technology, which enables the creation of a genetically varied group of banana mutants. This complex procedure entails subjecting the mutants to further stress screening utilizing L-Hyp in order to identify those exhibiting improved resistance to cold. This study conducted a comprehensive optimization of the screening conditions for EMS mutagenesis and L-Hyp, resulting in the identification of the mutant cm784, which exhibited remarkable cold resistance. Subsequent investigations further elucidated the physiological and transcriptomic responses of cm784 to low-temperature stress. RESULTS: EMS mutagenesis had a substantial effect on banana seedlings, resulting in modifications in shoot and root traits, wherein a majority of seedlings exhibited delayed differentiation and limited elongation. Notably, mutant leaves displayed altered biomass composition, with starch content exhibiting the most pronounced variation. The application of L-Hyp pressure selection aided in the identification of cold-resistant mutants among seedling-lethal phenotypes. The mutant cm784 demonstrated enhanced cold resistance, as evidenced by improved survival rates and reduced symptoms of chilling injury. Physiological analyses demonstrated heightened activities of antioxidant enzymes and increased proline production in cm784 when subjected to cold stress. Transcriptome analysis unveiled 946 genes that were differentially expressed in cm784, with a notable enrichment in categories related to 'Carbohydrate transport and metabolism' and 'Secondary metabolites biosynthesis, transport, and catabolism'. CONCLUSION: The present findings provide insights into the molecular mechanisms that contribute to the heightened cold resistance observed in banana mutants. These mechanisms encompass enhanced carbohydrate metabolism and secondary metabolite biosynthesis, thereby emphasizing the adaptive strategies employed to mitigate the detrimental effects induced by cold stress.


Assuntos
Musa , Musa/metabolismo , Metanossulfonato de Etila/metabolismo , Metanossulfonato de Etila/farmacologia , Biomassa , Perfilação da Expressão Gênica , Mutagênese , Fenótipo , Temperatura Baixa , Regulação da Expressão Gênica de Plantas
12.
Sci Rep ; 14(1): 3220, 2024 02 08.
Artigo em Inglês | MEDLINE | ID: mdl-38332256

RESUMO

The egg parasitoids of the genus Trichogramma are important potential biological control agents for a wide range of lepidopteran pests. Cold storage of host eggs has been proposed as a valuable technique for ensuring the release of sufficient parasitoid numbers whenever it is needed. In this context, the impact of low temperatures to induce quiescence in T. evanescens Westwood and T. chilonis Ishii was studied using eggs of Indian meal moth Plodia interpunctella (Hübner). Prepupae of the parasitoids were stored for 15, 30, 45, 60 and 75 d at 4 °C, following a 7 d period of acclimation at 10 °C. Both parasitoid species seem to survive unfavorable temperature conditions by entering a state of quiescence. Parasitism, adult emergence, sex ratio and progeny quality were not affected by cold storage in either parasitoid species for up to 30 d of storage. Parasitized host eggs of P. interpunctella can be stored for up to 60 d at 4 °C for both parasitoids, but there was no emergence at 75 d. General productivity values gradually decreased as the duration of storage lengthened for both species. Our results clearly reveal that the eggs parasitized by these species can be stored for up to 30 d at 4 °C in a state of quiescence without much loss of their performance compared to the control eggs.


Assuntos
Himenópteros , Mariposas , Vespas , Animais , Temperatura , Temperatura Baixa , Agentes de Controle Biológico
13.
Environ Health Perspect ; 132(2): 27004, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38334741

RESUMO

BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a heterogeneous pulmonary disease affecting 16 million Americans. Individuals with COPD are susceptible to environmental disturbances including heat and cold waves that can exacerbate disease symptoms. OBJECTIVE: Our objective was to estimate heat and cold wave-associated mortality risks within a population diagnosed with a chronic respiratory disease. METHODS: We collected individual level data with geocoded residential addresses from the Veterans Health Administration on 377,545 deceased patients with COPD (2016 to 2021). A time stratified case-crossover study was designed to estimate the incidence rate ratios (IRR) of heat and cold wave mortality risks using conditional logistic regression models examining lagged effects up to 7 d. Attributable risks (AR) were calculated for the lag day with the strongest association for heat and cold waves, respectively. Effect modification by age, gender, race, and ethnicity was also explored. RESULTS: Heat waves had the strongest effect on all-cause mortality at lag day 0 [IRR: 1.04; 95% confidence interval (CI): 1.02, 1.06] with attenuated effects by lag day 1. The AR at lag day 0 was 651 (95% CI: 326, 975) per 100,000 veterans. The effect of cold waves steadily increased from lag day 2 and plateaued at lag day 4 (IRR: 1.04; 95% CI: 1.02, 1.07) with declining but still elevated effects over the remaining 7-d lag period. The AR at lag day 4 was 687 (95% CI: 344, 1,200) per 100,000 veterans. Differences in risk were also detected upon stratification by gender and race. DISCUSSION: Our study demonstrated harmful associations between heat and cold waves among a high-risk population of veterans with COPD using individual level health data. Future research should emphasize using individual level data to better estimate the associations between extreme weather events and health outcomes for high-risk populations with chronic medical conditions. https://doi.org/10.1289/EHP13176.


Assuntos
Doença Pulmonar Obstrutiva Crônica , Veteranos , Humanos , Estados Unidos/epidemiologia , Temperatura Alta , Estudos Cross-Over , Temperatura Baixa , Doença Pulmonar Obstrutiva Crônica/epidemiologia , Mortalidade
14.
Int J Mol Sci ; 25(3)2024 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-38338824

RESUMO

In nature, plants are exposed to a range of climatic conditions. Those negatively impacting plant growth and survival are called abiotic stresses. Although abiotic stresses have been extensively studied separately, little is known about their interactions. Here, we investigate the impact of long-term mild metal exposure on the cold acclimation of Salix viminalis roots using physiological, transcriptomic, and proteomic approaches. We found that, while metal exposure significantly affected plant morphology and physiology, it did not impede cold acclimation. Cold acclimation alone increased glutathione content and glutathione reductase activity. It also resulted in the increase in transcripts and proteins belonging to the heat-shock proteins and related to the energy metabolism. Exposure to metals decreased antioxidant capacity but increased catalase and superoxide dismutase activity. It also resulted in the overexpression of transcripts and proteins related to metal homeostasis, protein folding, and the antioxidant machinery. The simultaneous exposure to both stressors resulted in effects that were not the simple addition of the effects of both stressors taken separately. At the antioxidant level, the response to both stressors was like the response to metals alone. While this should have led to a reduction of frost tolerance, this was not observed. The impact of the simultaneous exposure to metals and cold acclimation on the transcriptome was unique, while at the proteomic level the cold acclimation component seemed to be dominant. Some genes and proteins displayed positive interaction patterns. These genes and proteins were related to the mitigation and reparation of oxidative damage, sugar catabolism, and the production of lignans, trehalose, and raffinose. Interestingly, none of these genes and proteins belonged to the traditional ROS homeostasis system. These results highlight the importance of the under-studied role of lignans and the ROS damage repair and removal system in plants simultaneously exposed to multiple stressors.


Assuntos
Lignanas , Metais Pesados , Salix , Antioxidantes/metabolismo , Salix/genética , Salix/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Proteômica , Metais Pesados/toxicidade , Metais Pesados/metabolismo , Plantas/metabolismo , Aclimatação , Lignanas/metabolismo , Temperatura Baixa
15.
Int J Mol Sci ; 25(3)2024 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-38338887

RESUMO

Vascularized composite allotransplantation (VCA) represents a promising reconstructive solution primarily conducted to improve quality of life. However, tissue damage caused by cold-ischemia (CI) storage prior to transplant represents a major factor limiting widespread application. This study investigates the addition of the novel free radical scavenger PrC-210 to UW Organ Preservation Solution (UW Solution) to suppress CI-induced skeletal muscle injury in a rat hind limb amputation model. Lewis rats received systemic perfusion of UW solution +/- PrC-210 (0 mM control, 10 mM, 20 mM, 30 mM, or 40 mM), followed by bilateral transfemoral amputation. Limbs were stored in 40 mL of the same perfusate at 4 °C for 48 h. Muscle punch biopsies were taken at set times over the 48 h cold-storage period and analyzed for caspase-3,7 activity, cytochrome C levels, and qualitative histology. A single 15 s perfusion of PrC-210-containing UW Solution conferred a dose-dependent reduction in CI-induced muscle cell death over 48 h. In the presence of PrC-210, muscle cell mitochondrial cytochrome C release was equivalent to 0 h controls, with profound reductions in the caspase-3,7 apoptotic marker that correlated with limb histology. PrC-210 conferred complete prevention of ROS-induced mitochondrial lysis in vitro, as measured by cytochrome C release. We conclude that the addition of 30 mM PrC210 to UW Solution conferred the most consistent reduction in CI limb damage, and it warrants further investigation for clinical application in the VCA setting.


Assuntos
Aloenxertos Compostos , Diaminas , Soluções para Preservação de Órgãos , Traumatismo por Reperfusão , Compostos de Sulfidrila , Ratos , Animais , Sequestradores de Radicais Livres , Caspase 3 , Aloenxertos Compostos/patologia , Citocromos c , Qualidade de Vida , Ratos Endogâmicos Lew , Glutationa/farmacologia , Alopurinol/farmacologia , Insulina/farmacologia , Isquemia , Preservação de Órgãos , Temperatura Baixa , Traumatismo por Reperfusão/patologia , Rafinose , Adenosina
16.
Food Res Int ; 179: 113968, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38342524

RESUMO

The rising demand for plant-based meat analogues as alternatives to animal products has sparked interest in understanding the complex interplay between their structural and mechanical properties. The ability to manipulate the processing parameters and protein blend composition offers fundamental insights into the texturization process and holds economic and sustainable implications for the food industry. Consequently, the correlation between mechanical and structural properties in meat analogues is crucial for achieving consumer satisfaction and successful market penetration, providing comprehensive insights into the textural properties of meat analogues and their potential to mimic traditional animal produce. Our study delves into the relationship between structural and mechanical anisotropy in meat analogues produced using high moisture extrusion cooking, which involves blending protein, water, and other ingredients, followed by a controlled heating and cooling process to achieve a fibrous texture akin to traditional meat. By employing techniques such as scanning small-angle X-ray scattering, scanning electron microscopy, and mechanical testing we investigate the fibrous structure and its impact on the final texture of meat analogues. We show that textural and structural anisotropy is reflected on the mechanical properties measured using tensile and dynamic mechanical techniques. It is demonstrated that the calculated anisotropy indexes, a measure for the degree of textural and structural anisotropy, increase with increasing protein content. Our findings have significant implications for the understanding and development of plant-based meat analogues with structures that can be tuned to closely resemble the animal meat textures of choice, thereby enabling consumers to transition to more sustainable dietary choices while preserving familiar eating habits.


Assuntos
Temperatura Baixa , Animais , Anisotropia , Culinária , Carne
17.
Extremophiles ; 28(1): 17, 2024 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-38342818

RESUMO

Yeasts from cold environments have a wide range of strategies to prevent the negative effects of extreme conditions, including the production of metabolites of biotechnological interest. We investigated the growth profile and production of metabolites in yeast species isolated from cold environments. Thirty-eight strains were tested for their ability to grow at different temperatures (5-30 °C) and solute concentrations (3-12.5% NaCl and 50% glucose). All strains tested were able to grow at 5 °C, and 77% were able to grow with 5% NaCl at 18 °C. We were able to group strains based on different physicochemical/lifestyle profiles such as polyextremotolerant, osmotolerant, psychrotolerant, or psychrophilic. Five strains were selected to study biomass and metabolite production (glycerol, trehalose, ergosterol, and mycosporines). These analyses revealed that the accumulation pattern of trehalose and ergosterol was related to each lifestyle profile. Also, our findings would suggest that mycosporines does not have a role as an osmolyte. Non-conventional fermentative yeasts such as Phaffia tasmanica and Saccharomyces eubayanus may be of interest for trehalose production. This work contributes to the knowledge of non-conventional yeasts with biotechnological application from cold environments, including their growth profile, metabolites, and biomass production under different conditions.


Assuntos
Basidiomycota , Trealose , Trealose/metabolismo , Cloreto de Sódio/metabolismo , Leveduras , Ergosterol/metabolismo , Temperatura Baixa
18.
Proc Natl Acad Sci U S A ; 121(8): e2301053120, 2024 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-38346186

RESUMO

While low-temperature Nuclear Magnetic Resonance (NMR) holds great promise for the analysis of unstable samples and for sensitizing NMR detection, spectral broadening in frozen protein samples is a common experimental challenge. One hypothesis explaining the additional linewidth is that a variety of conformations are in rapid equilibrium at room temperature and become frozen, creating an inhomogeneous distribution at cryogenic temperatures. Here, we investigate conformational heterogeneity by measuring the backbone torsion angle (Ψ) in Escherichia coli Dihydrofolate Reductase (DHFR) at 105 K. Motivated by the particularly broad N chemical shift distribution in this and other examples, we modified an established NCCN Ψ experiment to correlate the chemical shift of Ni+1 to Ψi. With selective 15N and 13C enrichment of Ile, only the unique I60-I61 pair was expected to be detected in 13C'-15N correlation spectrum. For this unique amide, we detected three different conformation basins based on dispersed chemical shifts. Backbone torsion angles Ψ were determined for each basin: 114 ± 7° for the major peak and 150 ± 8° and 164 ± 16° for the minor peaks as contrasted with 118° for the X-ray crystal structure (and 118° to 130° for various previously reported structures). These studies support the hypothesis that inhomogeneous distributions of protein backbone torsion angles contribute to the lineshape broadening in low-temperature NMR spectra.


Assuntos
Temperatura Baixa , Proteínas , Temperatura , Espectroscopia de Ressonância Magnética , Conformação Proteica , Proteínas/química , Ressonância Magnética Nuclear Biomolecular
19.
J Exp Biol ; 227(4)2024 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-38299309

RESUMO

In nature, many organisms experience a daily range of body temperatures. Thermal performance at stable temperatures is often extrapolated to predict function in cyclical environments. However, temperature order and cyclicity may influence physiological processes. The current study compared energy intake, digestive passage time and energy budgets at a stable temperature (33°C) and two temperature cycles in lizards (Sceloporus consobrinus), to determine (1) whether stable treatments adequately project performance in a cycling environment and (2) whether temperature order influences performance. Cycles had a mean temperature of 33°C, and rotated through 30°C, 33°C and 36°C daily, with equal durations of time at each temperature but differing temperature order, with warm days and cool nights in cycle 1 and cool days and warm nights in cycle 2. For analyses, performance in the stable treatment was compared with that during cycles. If temperature is the primary factor regulating performance, then performance from the stable treatment and cycles should compare favorably. However, physiological performance varied based on temperature treatment. Energy intake and budgets were similar between the stable trial and cycle 1 but not cycle 2. However, passage time did not differ. Notably, the two cycling regimes consistently varied in performance, indicating that temperature order plays a primary role in regulating performance. Physiological data collection requires careful consideration of effects of cycling versus stable temperature treatments. Stable temperatures do not consistently represent performance in cycling regimes and consideration should be paid not only to which temperatures animals experience but also to how temperature is experienced in nature.


Assuntos
Lagartos , Animais , Temperatura , Lagartos/fisiologia , Pradaria , Temperatura Corporal , Temperatura Baixa
20.
Sci Total Environ ; 918: 170448, 2024 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-38301774

RESUMO

In the past decade, there has been a significant rise in sustainable biomass based biofuel production to address energy needs while mitigating environmental impacts. Traditionally, bioethanol was used for biofuel production, but concerns over food security and environmental preservation have led to growing interest in alternative sources such as neutral lipids from vegetable oil and microalgae for biodiesel production. This research paper evaluates the potential of various oleaginous plants and microalgae as feedstocks for biodiesel production, with a focus on their fatty acid composition and its impact on biodiesel properties. The study examines the fatty acid profiles of 43 different plant and microalgae species and employs various equations to estimate key physical properties of biodiesel. Additionally, the communication compares these properties to International Biodiesel Standards (EN 14214 and ASTM D6751-08) to assess the suitability of the derived biodiesel for commercial use. It is impossible to describe a single composition that is optimal in terms of all essential fuel properties due to the opposing effects of some structural features of the Fatty Acid Methyl Esters (FAME). However, biodiesel should contain relatively low concentrations of both long chain saturated and polyunsaturated FAME to ensure adequate efficiency in terms of low temperature operability and oxidative stability. The results reveal significant variations in properties amongst different feedstocks, highlighting the importance of feedstock selection in biodiesel production. The study also establishes correlations between various fuel properties, providing valuable insights in to optimizing biodiesel production processes, which will be of great use to researchers, engineers, and stakeholders involved in biodiesel production.


Assuntos
Ácidos Graxos , Microalgas , Ácidos Graxos/química , Biocombustíveis , Óleos de Plantas/química , Temperatura Baixa , Biomassa
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