Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.847
Filtrar
1.
Rev Fac Cien Med Univ Nac Cordoba ; 81(3): 552-569, 2024 09 27.
Artigo em Espanhol | MEDLINE | ID: mdl-39352854

RESUMO

Background: spinal cord injury leaves as a sequel in the acute and chronic period, deficiencies in the stability of joint functions and in the function of voluntary movement control. Those with good trunk control have a greater probability of carrying out activities of daily living by themselves; up to now, we do not have reliable tools in Argentina that evaluate trunk control in these subjects. Objectives: to cross-culturally adapt the trunk control test to Argentinean Spanish in subjects with sequelae of spinal cord injury and to establish interobserver and intraobserver reliability. Methodology: the subjects were admitted using a non-probabilistic convenience sampling. Subjects who were between 15 and 75 years old and had a diagnosis of spinal cord injury were included. Subjects who had: another neurological diagnosis, alteration in sensory organs, conditions that prevent the performance of the test, and psychiatric illness were excluded. Results: 30 subjects were included for cross-cultural adaptation and 55 for reliability. Semantic modifications were made to all items and response options. The intraobserver and interobserver reliability of the scale or subdomains did not achieve a sufficient score. Conclusion: the trunk control test was adapted cross-culturally to Argentine Spanish and interobserver and intraobserver reliability was established. The adaptation was achieved through semantic changes and the reliability was not sufficient. In the future, studies should be carried out to improve the reliability and study the validity of the tool.


Introducción: la lesión de la médula espinal deja como secuela en el período agudo y crónico, deficiencias en la estabilidad de las funciones articulares y de la función del control de los movimientos voluntarios. Aquellos con buen control de tronco poseen una mayor probabilidad de realizar por sí mismos  actividades de la vida diaria, hasta el momento, no contamos en Argentina con herramientas fiables  que evalúen el control de tronco en estos sujetos. Objetivos: adaptar transculturalmente al castellano argentino el trunk control test  en sujetos con secuela de lesión medular espinal y establecer la fiabilidad interobservador, intraobservador. Metodología: los sujetos fueron ingresados mediante un muestreo no probabilístico  por conveniencia. Se incluyeron sujetos que: posean entre 15 años a 75 años y tengan diagnóstico de lesión medular espinal, se excluyeron sujetos que posean: otro diagnóstico neurológico, alteración en los órganos sensoriales, condiciones que impidan la realización de la prueba y enfermedad psiquiátrica. Resultados: 30 sujetos fueron incluidos para la adaptación transcultural y 55 para la fiabilidad. Se realizaron modificaciones semánticas en todos los ítems y opciones de respuesta. La fiabilidad intraobservador e interobservador de la escala o de los subdominios no logró un puntaje suficiente. Conclusión:  se adaptó transculturalmente al castellano argentino el trunk control test y se estableció la fiabilidad interobservador, intraobservador. La adaptación se logró a través de cambios semánticos y la fiabilidad no fue suficiente. A futuro se deberán realizar estudios para mejorar la fiabilidad y estudiar la validez de la herramienta.


Assuntos
Traumatismos da Medula Espinal , Traduções , Humanos , Traumatismos da Medula Espinal/fisiopatologia , Traumatismos da Medula Espinal/complicações , Argentina , Adulto , Reprodutibilidade dos Testes , Pessoa de Meia-Idade , Feminino , Masculino , Adolescente , Idoso , Adulto Jovem , Comparação Transcultural , Variações Dependentes do Observador , Tronco/fisiopatologia , Avaliação da Deficiência , Inquéritos e Questionários/normas , Atividades Cotidianas
2.
Clinics (Sao Paulo) ; 79: 100509, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39393277

RESUMO

STUDY DESIGN: Experimental study utilizing with a standardized model (MASCIS Impactor) of Spinal Cord Injury (SCI) in Balb C mouse model with implantation of mononuclear stem cells derived from the human umbilical cord and placenta blood in the early chronic phase of SCI. OBJECTIVES: The aim of this study was to evaluate the nerve regeneration and motor functional recovery in Balb C mice with surgically induced paraplegia in response to the use of mononuclear stem cells, in early chronic phase (> 2 weeks and < 6 months), because there is yet potential of neuronal and functional recovery as the neuronal scar is not still completely established. METHODS: Forty-eight mice were randomly assigned to 6 groups of 8 animals. Group 1 received the stem cells 3 weeks after the trauma, and Group 2 received them six weeks later. In Group 3, saline solution was injected at the site of the lesion 3 weeks after the trauma, and in Group 4, 6 weeks later. Group 5 underwent only spinal cord injury and Group 6 underwent laminectomy only. The scales used for motor assessment were BMS and MFS for 12 weeks. RESULTS: The intervention groups showed statistically significant motor improvement. In the histopathological analysis, the intervention groups had a lower degree of injury (p < 0.05). Regarding axonal budding, the intervention groups showed increasing in axonal budding in the caudal portion (p < 0.05). CONCLUSIONS: The use of stem cells in mice in the chronic phase after 3 and 6 weeks of SCI brings functional and histopathological benefits to them.


Assuntos
Modelos Animais de Doenças , Camundongos Endogâmicos BALB C , Regeneração Nervosa , Placenta , Distribuição Aleatória , Recuperação de Função Fisiológica , Traumatismos da Medula Espinal , Animais , Traumatismos da Medula Espinal/fisiopatologia , Feminino , Camundongos , Humanos , Gravidez , Fatores de Tempo , Regeneração Nervosa/fisiologia , Paraplegia/fisiopatologia , Transplante de Células-Tronco de Sangue do Cordão Umbilical/métodos , Atividade Motora/fisiologia , Cordão Umbilical/citologia , Masculino
4.
Int J Mol Sci ; 25(16)2024 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-39201729

RESUMO

Traumatic spinal cord injury is a major cause of disability for which there are currently no fully effective treatments. Recent studies using epidural electrical stimulation have shown significant advances in motor rehabilitation, even when applied during chronic phases of the disease. The present study aimed to investigate the effectiveness of epidural electric stimulation in the motor recovery of rats with spinal cord injury. Furthermore, we aimed to elucidate the neurophysiological mechanisms underlying motor recovery. First, we improved upon the impact spinal cord injury model to cause severe and permanent motor deficits lasting up to 2 months. Next, we developed and tested an implantable epidural spinal cord stimulator device for rats containing an electrode and an implantable generator. Finally, we evaluated the efficacy of epidural electrical stimulation on motor recovery after spinal cord injury in Wistar rats. A total of 60 animals were divided into the following groups: (i) severe injury with epidural electrical stimulation (injury + stim, n = 15), (ii) severe injury without stimulation (group injury, n = 15), (iii) sham implantation without battery (sham, n = 15), and (iv) a control group, without surgical intervention (control, n = 15). All animals underwent weekly evaluations using the Basso, Beattie, Bresnahan (BBB) locomotor rating scale index, inclined plane, and OpenField test starting one week before the lesion and continuing for eight weeks. After this period, the animals were sacrificed and their spinal cords were explanted and prepared for histological analysis (hematoxylin-eosin) and immunohistochemistry for NeuN, ß-III-tubulin, synaptophysin, and Caspase 3. Finally, NeuN-positive neuronal nuclei were quantified through stereology; fluorescence signal intensities for ß-tubulin, synaptophyin, and Caspase 3 were quantified using an epifluorescence microscope. The injury + stim group showed significant improvement on the BBB scale compared with the injured group after the 5th week (p < 0.05). Stereological analysis showed a significantly higher average count of neural cells in the injury + stim group in relation to the injury group (1783 ± 2 vs. 897 ± 3, p < 0.001). Additionally, fluorescence signal intensity for synaptophysin was significantly higher in the injury + stim group in relation to the injury group (1294 ± 46 vs. 1198 ± 23, p < 0.01); no statistically significant difference was found in ß-III-tubulin signal intensity. Finally, Caspase 3 signal intensity was significantly lower in the stim group (727 ± 123) compared with the injury group (1225 ± 87 p < 0.05), approaching levels observed in the sham and control groups. Our data suggest a regenerative and protective effect of epidural electrical stimulation in rats subjected to impact-induced traumatic spinal cord injury.


Assuntos
Modelos Animais de Doenças , Plasticidade Neuronal , Ratos Wistar , Traumatismos da Medula Espinal , Animais , Traumatismos da Medula Espinal/terapia , Traumatismos da Medula Espinal/fisiopatologia , Traumatismos da Medula Espinal/patologia , Ratos , Recuperação de Função Fisiológica , Terapia por Estimulação Elétrica/métodos , Sinaptofisina/metabolismo , Tubulina (Proteína)/metabolismo , Espaço Epidural/patologia , Medula Espinal/metabolismo , Medula Espinal/patologia , Medula Espinal/fisiopatologia , Masculino , Caspase 3/metabolismo , Regeneração Nervosa , Feminino , Proteínas do Tecido Nervoso , Antígenos Nucleares
5.
Biol Res ; 57(1): 53, 2024 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-39135103

RESUMO

BACKGROUND: As a common disabling disease, irreversible neuronal death due to spinal cord injury (SCI) is the root cause of functional impairment; however, the capacity for neuronal regeneration in the developing spinal cord tissue is limited. Therefore, there is an urgent need to investigate how defective neurons can be replenished and functionally integrated by neural regeneration; the reprogramming of intrinsic cells into functional neurons may represent an ideal solution. METHODS: A mouse model of transection SCI was prepared by forceps clamping, and an adeno-associated virus (AAV) carrying the transcription factors NeuroD1 and Neurogenin-2(Ngn2) was injected in situ into the spinal cord to specifically overexpress these transcription factors in astrocytes close to the injury site. 5-bromo-2´-deoxyuridine (BrdU) was subsequently injected intraperitoneally to continuously track cell regeneration, neuroblasts and immature neurons marker expression, neuronal regeneration, and glial scar regeneration. In addition, immunoprotein blotting was used to measure the levels of transforming growth factor-ß (TGF-ß) pathway-related protein expression. We also evaluated motor function, sensory function, and the integrity of the blood-spinal cord barrier(BSCB). RESULTS: The in situ overexpression of NeuroD1 and Ngn2 in the spinal cord was achieved by specific AAV vectors. This intervention led to a significant increase in cell regeneration and the proportion of cells with neuroblasts and immature neurons cell properties at the injury site(p < 0.0001). Immunofluorescence staining identified astrocytes with neuroblasts and immature neurons cell properties at the site of injury while neuronal marker-specific staining revealed an increased number of mature astrocytes at the injury site. Behavioral assessments showed that the intervention did not improve The BMS (Basso mouse scale) score (p = 0.0726) and gait (p > 0.05), although the treated mice had more sensory sensitivity and greater voluntary motor ability in open field than the non-intervention mice. We observed significant repair of the BSCB at the center of the injury site (p < 0.0001) and a significant improvement in glial scar proliferation. Electrophysiological assessments revealed a significant improvement in spinal nerve conduction (p < 0.0001) while immunostaining revealed that the levels of TGF-ß protein at the site of injury in the intervention group were lower than control group (p = 0.0034); in addition, P70 s6 and PP2A related to the TGF-ß pathway showed ascending trend (p = 0.0036, p = 0.0152 respectively). CONCLUSIONS: The in situ overexpression of NeuroD1 and Ngn2 in the spinal cord after spinal cord injury can reprogram astrocytes into neurons and significantly enhance cell regeneration at the injury site. The reprogramming of astrocytes can lead to tissue repair, thus improving the reduced threshold and increasing voluntary movements. This strategy can also improve the integrity of the blood-spinal cord barrier and enhance nerve conduction function. However, the simple reprogramming of astrocytes cannot lead to significant improvements in the striding function of the lower limbs.


Assuntos
Astrócitos , Fatores de Transcrição Hélice-Alça-Hélice Básicos , Modelos Animais de Doenças , Proteínas do Tecido Nervoso , Traumatismos da Medula Espinal , Animais , Traumatismos da Medula Espinal/terapia , Traumatismos da Medula Espinal/fisiopatologia , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Astrócitos/fisiologia , Proteínas do Tecido Nervoso/metabolismo , Camundongos , Regeneração Nervosa/fisiologia , Neurônios , Feminino , Camundongos Endogâmicos C57BL , Medula Espinal/metabolismo
6.
J Med Case Rep ; 18(1): 386, 2024 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-39152447

RESUMO

BACKGROUND: Experimental studies have shown that repetitive trans-spinal magnetic stimulation (TsMS) decreases demyelination and enables recovery after spinal cord injury (SCI). However, the usefulness of TsMS in humans with SCI remains unclear. Therefore, the main objective of this study is to evaluate the effects of TsMS combined with kinesiotherapy on SCI symptoms. We describe a protocol treatment with TsMS and kinesiotherapy in a patient with SCI due to neuromyelitis optica (NMO)-associated transverse myelitis. CASE PRESENTATION: A 23-year-old white male with NMO spectrum disorders started symptoms in 2014 and included lumbar pain evolving into a mild loss of strength and sensitivity in both lower limbs. Five months later, the symptoms improved spontaneously, and there were no sensorimotor deficits. Two years later, in 2016, the symptoms recurred with a total loss of strength and sensitivity in both lower limbs. Initially, physiotherapy was provided in 15 sessions with goals of motor-sensory recovery and improving balance and functional mobility. Subsequently, TsMS (10 Hz, 600 pulses, 20-seconds inter-trains interval, at 90% of resting motor threshold of the paravertebral muscle) was applied at the 10th thoracic vertebral spinous process before physiotherapy in 12 sessions. Outcomes were assessed at three time points: prior to physiotherapy alone (T-1), before the first session of TsMS combined with kinesiotherapy (T0), and after 12 sessions of TsMS combined with kinesiotherapy (T1). The patient showed a 25% improvement in walking independence, a 125% improvement in balance, and an 18.8% improvement in functional mobility. The Patient Global Impression of Change Scale assessed the patient's global impression of change as 'much improved'. CONCLUSION: TsMS combined with kinesiotherapy may safely and effectively improve balance, walking independence, and functional mobility of patients with SCI due to NMO-associated transverse myelitis.


Assuntos
Magnetoterapia , Neuromielite Óptica , Recuperação de Função Fisiológica , Traumatismos da Medula Espinal , Humanos , Masculino , Neuromielite Óptica/terapia , Neuromielite Óptica/complicações , Adulto Jovem , Magnetoterapia/métodos , Traumatismos da Medula Espinal/complicações , Traumatismos da Medula Espinal/reabilitação , Traumatismos da Medula Espinal/terapia , Resultado do Tratamento , Terapia Combinada , Modalidades de Fisioterapia
7.
Brain Topogr ; 37(6): 1232-1241, 2024 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-39162868

RESUMO

The purpose of this study is to evaluate the efficacy of repetitive transcranial magnetic stimulation (rTMS) combined with body weight-support treadmill training (BWSTT) for improving walking function of individuals with chronic incomplete spinal cord injury (iSCI). A 4-week, double-blinded, randomized, sham-controlled pilot study involved 12 sessions of real (10 Hz, 1800 pulses) or sham rTMS combined with BWSTT (15-20 min, moderate intensity). Walking independence was assessed using the Walking Index for Spinal Cord Injury II (WISCI-II). Lower extremity motor function (lower extremity motor score [LEMS]) and spasticity, sensory function, functional independence (Spinal Cord Injury Measure III [SCIM-III]), and quality of life were also assessed. Walking independence (WISCI-II) after the 6th session was higher in the BWSTT/rTMS real (n = 7) (median change (IQR): 3 (1.5 to 3.5)) than in the sham group (n = 8) (median change (IQR): 0 (0 to 0.25), but there was no difference between groups after 12th session (BWSTT/rTMS real median change (IQR): 4 (2 to 5); BWSSTT/rTMS sham median change (IQR): 0 (0 to 3.25). Compared to baseline, LEMS and SCIM-III mobility scores were increased after 12 sessions in the BWSTT/rTMS real but not in the sham group. Within- and between-group sensory function, functional independence, and quality of life remained similar. This preliminary result suggests that combining BWSTT with rTMS could lead to earlier gait improvement in patients with chronic iSCI.


Assuntos
Terapia por Exercício , Traumatismos da Medula Espinal , Estimulação Magnética Transcraniana , Caminhada , Humanos , Traumatismos da Medula Espinal/fisiopatologia , Traumatismos da Medula Espinal/reabilitação , Traumatismos da Medula Espinal/terapia , Projetos Piloto , Masculino , Feminino , Estimulação Magnética Transcraniana/métodos , Caminhada/fisiologia , Pessoa de Meia-Idade , Adulto , Método Duplo-Cego , Terapia por Exercício/métodos , Peso Corporal/fisiologia , Resultado do Tratamento , Qualidade de Vida , Doença Crônica
8.
Physiother Res Int ; 29(4): e2115, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39104156

RESUMO

INTRODUCTION: The revised international standards for neurological classification of spinal cord injury (ISNCSCI) have facilitated the documentation of non-spinal cord injury-related impairments, such as chronic peripheral nerve injuries and muscle weakness due to immobility. This advancement addresses potential biases in muscle strength examinations. Utilizing electrically evoked contractions from paralyzed muscles, enhanced by electrodiagnosis, holds promise in identifying false-negative diagnoses of non-responsiveness to neuromuscular electrical stimulation. This concept prompts the exploration of polyneuromyopathy arising from nonuse in paralyzed muscles. CASE SERIES PRESENTATION: To substantiate our hypothesis, we recruited nine participants for a case series aimed at elucidating the potential benefits of incorporating the stimulus electrodiagnostic test (SET) to mitigate non-responsiveness during preparation for functional electrical stimulation (FES)-assisted cycling. In our convenience sample (n = 5), we conducted neurological mapping based on ISNCSCI and applied SET on the quadriceps. The SET guided optimal dosimetry for evoking contractions and revealed responses similar to those observed in peripheral neuropathies, with α coefficients equal to or lower than 2.00. This observation is likely attributable to nonuse of paralyzed muscles, indicative of an ongoing polyneuropathy in individuals with chronic spinal cord injury (SCI). DISCUSSION: Among the nine initially recruited subjects, seven exhibited responsiveness to neuromuscular electrical stimulation (78% responsiveness), with two participants excluded based on exclusion criteria. In the final five reported cases, all displayed α coefficient values indicating impaired neuromuscular accommodation, and one presented no α coefficient within the normal range. The inclusion of electrodiagnosis appears effective in averting non-responsiveness, suggesting the presence of ongoing polyneuropathies in paralyzed muscles.


Assuntos
Eletrodiagnóstico , Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Adulto , Polineuropatias/diagnóstico , Estimulação Elétrica , Traumatismos da Medula Espinal/diagnóstico , Traumatismos da Medula Espinal/reabilitação , Traumatismos da Medula Espinal/complicações , Eletromiografia , Contração Muscular/fisiologia , Debilidade Muscular/diagnóstico , Idoso , Músculo Esquelético
9.
Artigo em Inglês | MEDLINE | ID: mdl-39063477

RESUMO

Spinal cord injury (SCI) is a condition that significantly affects the quality of life (QoL) of individuals, causing motor, physiological, social, and psychological impairments. Physical exercise plays a crucial role in maintaining the health and functional capacity of these individuals, helping to minimize the negative impacts of SCI. The aim of this study was to evaluate the effect of detraining (DT) (reduction or cessation of physical exercise) during the pandemic on five individuals with thoracic SCI. We assessed muscle strength using strength tests, functional capacity using a functional agility test, mental health using anxiety and depression inventories, and body composition using dual-energy X-ray absorptiometry (DEXA). The results after 33 months of DT showed significant losses in functional agility and MS, as well as a worsening in symptoms of anxiety and depression. It was observed that total body mass and fat mass (FM) exhibited varied behaviors among the individuals. Similarly, the results for lean body mass were heterogeneous, with one participant showing significant deterioration. It is concluded that DT caused by the pandemic worsened the physical and mental condition of individuals with SCI, highlighting the importance of continuous exercise for this population and underscoring the need for individual assessments to fully understand the impacts of DT.


Assuntos
Composição Corporal , Saúde Mental , Força Muscular , Traumatismos da Medula Espinal , Humanos , Traumatismos da Medula Espinal/fisiopatologia , Traumatismos da Medula Espinal/psicologia , Masculino , Adulto , Pessoa de Meia-Idade , Feminino , COVID-19/psicologia , Exercício Físico , Qualidade de Vida , Ansiedade/fisiopatologia , Depressão/fisiopatologia
12.
Spinal Cord ; 62(8): 468-478, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38907085

RESUMO

STUDY DESIGN: Mixed-method approach. OBJECTIVES: To develop and assess the content validity of a semi-structured interview that captures the lived experience of using a manual wheelchair among individuals with SCI in the real world, the Wheelchair Mobility Activity Log (WC-MAL). SETTING: SCIR-Group (UDESC)/Brazil. METHODS: Developing the WC-MAL comprised five steps: (1) defining the construct-based on the International Classification of Functioning, Disability and Health (ICF); (2) identifying relevant activities from other assessment instruments and interviews with the end-users (14 Individuals with SCI and 13 rehabilitation professionals); (3) Selecting the items - activities were linked to ICF codes and grouped into sets; (4) developing the scoring scales based on interviews with the end-users; and, (5) evaluating content validity in accordance with the COnsensus-based Standards for the selection of health Measurement INstruments (COSMIN). The content validity ratio (CVR) for each item and scale and the overall instrument content validity index (CVI) were calculated. RESULTS: From an initial draft of 295 activities identified, a set of 222 activities was linked to the ICF domain of "Mobility (d4)" and further refined to generate the 23 items in the WC-MAL. Three scales were developed to assess Frequency (how often), Performance (how well), and Assistance (assistance needed) levels. The items and scales showed a CVR superior to the critical value established (≥0.64). The general CVI value was 0.96. CONCLUSION: The WC-MAL is a promising clinical instrument with adequate content validity to assess the spontaneous use of the manual wheelchair in the real world among individuals with SCI.


Assuntos
Traumatismos da Medula Espinal , Cadeiras de Rodas , Humanos , Traumatismos da Medula Espinal/reabilitação , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Reprodutibilidade dos Testes , Atividades Cotidianas , Avaliação da Deficiência , Entrevistas como Assunto , Pessoas com Deficiência/reabilitação , Brasil
13.
J Bodyw Mov Ther ; 39: 285-292, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38876640

RESUMO

INTRODUCTION: Spinal cord injuries (SCI) have physiological, emotional, and economic consequences in the lives of affected people. Resistance training (RT) is efficient in improving several physiological factors, quality of life, and body composition. Due to the scarce literature on the analysis of isolated RT, the objective of this systematic review is to evaluate the effects of RT without the association of other techniques, in aspects related to the quality of life and body composition of people with SCI. EVIDENCE ACQUISITION: The research was carried out in databases such as Pubmed, Cochrane, and Web of Science using the terms ("Spinal cord injury") AND (("Resistance Training") OR ("Strength training")). Given the lack of evidence on the subject, no deadline was set for the study to be eligible for analysis. EVIDENCE SYNTHESIS: The search for the articles was carried out in November of 2023 and returned 470 results, of which 315 remained after the elimination of duplicates, with 281 being excluded after title analysis. A total of 34 abstracts were analyzed and 29 studies were excluded, leaving 5 complete articles for thorough analysis. CONCLUSIONS: After analyzing the main results, we concluded that RT promotes significant improvements in body composition, pain, stress and depression symptoms, increased functionality, physical awareness, and quality of life.


Assuntos
Composição Corporal , Qualidade de Vida , Treinamento Resistido , Traumatismos da Medula Espinal , Humanos , Traumatismos da Medula Espinal/reabilitação , Traumatismos da Medula Espinal/psicologia , Treinamento Resistido/métodos , Composição Corporal/fisiologia , Depressão/terapia
14.
Int. j. morphol ; 42(3): 709-717, jun. 2024. ilus
Artigo em Inglês | LILACS | ID: biblio-1564595

RESUMO

SUMMARY: Spinal cord injury (SCI) usually arises from compression due to traffic accidents and falls, resulting in varying degrees of movement, sensory loss, and possible paralysis. Glabridin (Gla) is a natural compound derived from licorice. It significantly affects drug development and medicine because of its anti-inflammatory, anti-oxidative, anti-tumoral, antibacterial, bone protective, cardiovascular protective, neuroprotective, liver protective, anti-obesity, and anti-diabetic properties. Various methods were employed to administer Gla to SCI mice in order to investigate its impact on the recovery of motor function. The mice were allocated into four cohorts using a randomization procedure. In the sham cohort, solely the lamina of vertebral arch was surgically exposed without causing any harm to the spinal cord tissue. Conversely, the injury cohort was subjected to spinal cord tissue damage and received no treatment thereafter. The mice in the remaining two cohorts received a dosage of 40 mg/kg Gla every two days via either intraperitoneal or intrathecal injection for a duration of 42 d following spinal cord injury. We conducted behavioral tests utilizing the Basso Mouse Scale score and gait analysis techniques. Magnetic resonance imaging and hematoxylin and eosin were employed to evaluate scar tissue formation. Systemic inflammation in mice was evaluated by employing an enzyme-linked immunosorbent assay. Gla promoted motor function recovery in mice following SCI and improved the pathological environment in the damaged area. These alterations were more evident in mice subjected to the intrathecal injection method. Intraperitoneal injections appear to be more beneficial for controlling systemic inflammatory responses. Although more intensive studies are required, Gla exhibits promising clinical potential as a cost-effective dietary phytochemical.


La lesión de la médula espinal (LME) generalmente surge de la compresión producto de caídas y accidentes de tránsito, lo que resulta en alteraciones del movimiento, pérdida sensorial y posible parálisis. La Glabridina (Gla) es un compuesto natural derivado del regaliz, constituyéndose en un aporte significativo para el desarrollo de fármacos y la medicina debido a sus propiedades antiinflamatorias, antioxidantes, antitumorales, antibacterianas, osteoprotectoras, cardioprotectoras, neuroprotectoras, hepatoprotectoras, antidiabéticas y contra la obesidad. En el presente trabajo se emplearon varios métodos para administrar Gla a ratones con lesión medular con el fin de investigar su impacto en la recuperación de la función motora. Los ratones fueron distribuidos en cuatro grupos mediante un procedimiento de aleatorización. En el grupo simulado, únicamente se expuso quirúrgicamente la lámina del arco vertebral sin causar ningún daño al tejido de la médula espinal. Por el contrario, el grupo lesionado fue sometido a daño del tejido de la médula espinal, sin recibir tratamiento posterior. Los ratones de los dos grupos restantes recibieron una dosis de 40 mg/kg de Gla cada dos días mediante inyección intraperitoneal o intratecal durante 42 días después de la lesión de la médula espinal. Fueron realizadas pruebas de comportamiento utilizando la puntuación de la escala Basso Mouse y técnicas de análisis de la marcha. Se emplearon imágenes por resonancia magnética y se aplicaron tinciones histológicas (Hematoxilina & Eosina) en muestras para evaluar la formación de tejido cicatricial. La inflamación sistémica en ratones se evaluó mediante el empleo de un ensayo inmunoabsorbente ligado a enzimas. Gla promovió la recuperación de la función motora en ratones después de una lesión medular y mejoró el entorno patológico en el área dañada. Estas alteraciones fueron más evidentes en ratones sometidos al método de inyección intratecal. Las inyecciones intraperitoneales parecen ser más beneficiosas para controlar las respuestas inflamatorias sistémicas. Aunque se requieren estudios más intensivos, Gla exhibe un potencial clínico prometedor como fitoquímico dietético rentable.


Assuntos
Animais , Feminino , Camundongos , Fenóis/administração & dosagem , Traumatismos da Medula Espinal/tratamento farmacológico , Isoflavonas/administração & dosagem , Ensaio de Imunoadsorção Enzimática , Sobrevivência Celular , Imunofluorescência , Fármacos Neuroprotetores , Recuperação de Função Fisiológica , Camundongos Endogâmicos C57BL , Atividade Motora/efeitos dos fármacos
15.
Rev. cuid. (En línea) ; 15(2): 1-18, 20240501.
Artigo em Inglês | LILACS, BDENF - Enfermagem, COLNAL | ID: biblio-1579012

RESUMO

Introduction: Considered an unpredictable and recurring problem, Neurogenic Bowel does not resolve over time and progressively worsens, translating into a physical and psychological challenge, significantly reducing Quality of Life. Objective: To construct and validate the face and content of an educational technology for use by nurses in the rehabilitation of Neurogenic Bowel in people with Spinal Cord Injury. Materials and methods: A methodological, quantitative study developed in two stages: the construction of an educational technology on the Canvas platform based on a literature review based on Wanda Horta's Theory of Human Needs and its validation by expert judges. The validation process included nurses with ability in neurogenic bowel in teaching, research or care. The criterion for validation was agreement of over 80%, analyzed using the Content Validation Index and binomial test with confidence intervals of 95.00% (p<0.05). Results: The integrative literature review proved necessary for the construction of the proposed educational technology and covered characteristics of the Neurogenic Bowel, bowel emptying techniques, guidelines and the systematization of nursing care. The protocol was validated by ten expert judges who had graduated in nursing for more than 10 years (100.00%), with an average age of 41.8 years, female (60.00%) and an average training period of 18.6 years. An overall Content Validation Index of 0.96 (p≤0.001) was obtained for the items assessed (objective, content, relevance, functionality, efficiency and appearance/diagramming). Discussion: The implementation of an intestinal rehabilitation program aims, above all, to achieve frequent, regular and consistent stools in people with spinal cord injury and nurses, as multipliers of knowledge, can be facilitators in the teaching-learning process for carers, people with this condition and other members of the healthcare team. Conclusion: It can be concluded that the educational technology has been validated and could help the teaching-learning process for nurses in the clinical practice of caring for people with Neurogenic Bowel Disease.


Introducción: Considerado un problema impredecible y recurrente, el Intestino Neurogénico no se resuelve por sí solo con el tiempo y se vuelve progresivamente más pronunciado, traduciéndose en un desafío físico y psicológico, reduciendo significativamente la Calidad de Vida. Objetivo: Construir y validar el rostro y el contenido de una tecnología educativa para uso del enfermero en la rehabilitación del Intestino Neurogénico en personas con Lesión Médular. Materiales y Métodos: Estudio metodológico, cuantitativo, desarrollado en dos etapas: construcción de una tecnología educativa en la plataforma Canvas a partir de una revisión de literatura basada en la Teoría de las Necesidades Humanas de Wanda Horta y validación por jueces expertos. Para la validación se incluyeron enfermeros con experiencia en Intestino Neurogénico en la docencia, investigación o asistencia. El criterio de validación fue la concordancia mayor al 80%, analizado mediante el Índice de Validación de Contenido y prueba binomial con intervalos de confianza al 95,00% (p<0,05). Resultados: La revisión integradora de la literatura resultó necesaria para la construcción de la tecnología educativa propuesta e incluyó características del Intestino Neurogénico, técnicas de vaciamiento intestinal, guías y la sistematización de los cuidados de enfermería. Diez jueces expertos graduados en enfermería hacen más de 10 años (100,00%), con edad promedio de 41,8 años, predominantemente del sexo femenino (60,00%) y tiempo promedio de formación de 18,6 años, validaron el protocolo. Se obtuvo un Índice de Validación de Contenido general de 0,96 (p≤0,001) en los ítems evaluados (objetivo, contenido, relevancia, funcionalidad, eficiencia y apariencia/diseño). Discusión: La implementación de un programa de rehabilitación intestinal tiene como objetivo, sobre todo, obtener deposiciones frecuentes, regulares y consistentes en personas con Lesión Médular y el enfermero como multiplicador de conocimientos puede ser un facilitador en el proceso de enseñanza-aprendizaje de los cuidadores, de las personas. con esta afección y para otros miembros del equipo de atención médica. Conclusión: Se concluye que la tecnología educativa está validada y puede facilitar el proceso de enseñanza-aprendizaje del enfermero en la práctica clínica del cuidado de personas con Intestino Neurogénico.


Introdução: Considerado um problema imprevisível e recorrente, o Intestino Neurogênico não se resolve com o tempo e se acentua progressivamente, traduzindo-se em um desafio de caráter físico e psicológico, diminuindo significativamente a Qualidade de Vida. Objetivo: Construir e validar face e conteúdo de uma tecnologia educativa para uso de enfermeiros na reabilitação do Intestino Neurogênico em pessoas com Lesão Medular. Materiais e métodos: Estudo metodológico, quantitativo, desenvolvido em duas etapas: construção de uma tecnologia educativa na plataforma Canvas a partir da revisão da literatura fundamentado na Teoria das Necessidades Humanas de Wanda Horta e validação dele por juízes experts. Para a validação foram incluídos enfermeiros com expertise em Intestino Neurogênico na docência, pesquisa ou assistência. O critério para validação foi concordância superior a 80%, analisada por meio do Índice de Validação de Conteúdo e teste binomial com intervalos de confiança em 95,00% (p<0,05). Resultados: A revisão integrativa da literatura mostrou-se necessária para a construção da tecnologia educativa proposta e contemplou características do Intestino Neurogênico, técnicas de esvaziamento intestinal, orientações e a sistematização da assistência de enfermagem. Validaram o protocolo dez juízes experts graduados em enfermagem há mais de 10 anos (100,00%), com idade média de 41,8 anos, predominantemente do sexo feminino (60,00%) e tempo médio de formação de 18,6 anos. Obteve-se Índice de Validação de Conteúdo geral de 0,96 (p≤0,001) nos itens avaliados (objetivo, conteúdo, relevância, funcionalidade, eficiência e aparência/diagramação). Discussão: A implementação de um programa de reabilitação intestinal objetiva, sobretudo, a obtenção fezes frequentes, regulares e consistentes em pessoas com Lesão Medular e o enfermeiro enquanto multiplicador de conhecimento pode ser um facilitador no processo de ensino aprendizagem para os cuidadores, para as pessoas com esta condição e para outros membros da equipe de saúde. Conclusão: Conclui-se que a tecnologia educativa está validada e poderá facilitar o processo de ensino-aprendizagem do enfermeiro na prática clínica de cuidado com pessoas com Intestino Neurogênico.


Assuntos
Reabilitação , Traumatismos da Medula Espinal , Enfermagem , Pessoas com Deficiência , Intestino Neurogênico
16.
Clinics (Sao Paulo) ; 79: 100359, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38657346

RESUMO

OBJECTIVE: The aim of this study was to evaluate the GSH effect on functional and histological recovery after experimental spinal cord injury in rats. METHODS: Forty Wistar rats were subjected to spinal cord injury through the Multicenter Animal Spinal Cord Injury Study (MASCIS) Impactor system. The rats were sorted and divided into four groups, as follows: Group 1 ‒ Laminectomy and spinal cord injury; Group 2 ‒ Laminectomy, spinal cord injury and Saline Solution (SS) 0.9%; Group 3 ‒ Laminectomy, spinal cord injury, and GSH; and Group 4 ‒ lLaminectomy without spinal cord injury. GSH and SS were administered intraperitoneally. Groups 1 and 4 received no intervention. RESULTS: The rats were evaluated for locomotor function recovery at seven different times by the Basso, Beattie, and Bresnahan (BBB) scale on days 2, 7, 14, 21, 28, 35, and 42 after the spinal cord injury. On day 42, the rats were sacrificed to analyze the histological findings of the injured spinal cord. In the group submitted to GSH, our experimental study revealed better functional scores on the BBB scale, horizontal ladder scale, and cranial and caudal axon count. The differences found were statistically significant in BBB scores and axonal count analysis. CONCLUSION: This study demonstrated that using glutathione in experimental spinal trauma can lead to better functional recovery and improved axonal regeneration rate in Wistar rats submitted to experimental spinal cord injury.


Assuntos
Modelos Animais de Doenças , Glutationa , Ratos Wistar , Recuperação de Função Fisiológica , Traumatismos da Medula Espinal , Animais , Traumatismos da Medula Espinal/fisiopatologia , Traumatismos da Medula Espinal/patologia , Fatores de Tempo , Laminectomia , Masculino , Medula Espinal/patologia , Medula Espinal/fisiopatologia , Distribuição Aleatória , Ratos , Axônios/patologia , Locomoção/fisiologia , Reprodutibilidade dos Testes , Atividade Motora/fisiologia , Resultado do Tratamento
17.
Exp Neurol ; 377: 114785, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38670250

RESUMO

Spinal cord injury (SCI) results from various mechanisms that damage the nervous tissue and the blood-brain barrier, leading to sensory and motor function loss below the injury site. Unfortunately, current therapeutic approaches for SCI have limited efficacy in improving patients outcomes. Galectin-3, a protein whose expression increases after SCI, influences the neuroinflammatory response by favoring pro-inflammatory M1 macrophages and microglia, while inhibiting pro-regenerative M2 macrophages and microglia, which are crucial for inflammation resolution and tissue regeneration. Previous studies with Galectin-3 knock-out mice demonstrated enhanced motor recovery after SCI. The M1/M2 balance is strongly influenced by the predominant lymphocytic profiles (Th1, Th2, T Reg, Th17) and cytokines and chemokines released at the lesion site. The present study aimed to investigate how the absence of galectin-3 impacts the adaptive immune system cell population dynamics in various lymphoid spaces following a low thoracic spinal cord compression injury (T9-T10) using a 30 g vascular clip for one minute. It also aimed to assess its influence on the functional outcome in wild-type (WT)and Galectin-3 knock-out (GALNEG) mice. Histological analysis with hematoxylin-eosin and Luxol Fast Blue staining revealed that WT and GALNEG animals exhibit similar spinal cord morphology. The absence of galectin-3 does not affect the common neuroanatomy shared between the groups prompting us to analyze outcomes between both groups. Following our crush model, both groups lost motor and sensory functions below the lesion level. During a 42-day period, GALNEG mice demonstrated superior locomotor recovery in the Basso Mouse Scale (BMS) gait analysis and enhanced motor coordination performance in the ladder rung walk test (LRW) compared to WT mice. GALNEG mice also exhibited better sensory recovery, and their electrophysiological parameters suggested a higher number of functional axons with faster nerve conduction. Seven days after injury, flow cytometry of thymus, spleen, and blood revealed an increased number of T Reg and Th2 cells, accompanied by a decrease in Th1 and Th17 cells in GALNEG mice. Immunohistochemistry conducted on the same day exhibited an increased number of Th2 and T Reg cells around the GALNEG's spinal cord lesion site. At 42-day dpi immunohistochemistry analyses displayed reduced astrogliosis and greater axon preservation in GALNEG's spinal cord seem as a reduction of GFAP immunostaining and an increase in NFH immunostaining, respectively. In conclusion, GALNEG mice exhibited better functional recovery attributed to the milder pro-inflammatory influence, compensated by a higher quantity of T Reg and Th2 cells. These findings suggest that galectin-3 plays a crucial role in the immune response after spinal cord injury and could be a potential target for clinical therapeutic interventions.


Assuntos
Galectina 3 , Camundongos Endogâmicos C57BL , Camundongos Knockout , Recuperação de Função Fisiológica , Traumatismos da Medula Espinal , Animais , Traumatismos da Medula Espinal/patologia , Traumatismos da Medula Espinal/metabolismo , Traumatismos da Medula Espinal/fisiopatologia , Recuperação de Função Fisiológica/fisiologia , Galectina 3/metabolismo , Galectina 3/genética , Camundongos , Linfócitos/metabolismo , Feminino , Masculino
18.
Spinal Cord ; 62(6): 336-342, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38609569

RESUMO

STUDY DESIGN: Using a cross-sectional design, we extracted sociodemographic and clinical data from 488 Spinal Cord Injury (SCI) patients during their initial assessment before receiving intensive rehabilitation treatment. OBJECTIVES: The primary objectives of this study were to ascertain the prevalence of cognitive impairment in the study sample and specify the key clinical and demographic predictors of cognitive functioning in SCI patients. SETTING: Lucy Montoro Rehabilitation Institute (LMRI), University of Sao Paulo, Sao Paulo, Brazil. METHODS: We utilized independent univariate and multivariate regression models with the Montreal Cognitive Assessment (MoCA) scale, adapted for individuals with visual impairment. Moreover, we consider scores from the execution tasks (visuospatial/executive) as the dependent variable. RESULTS: Our findings demonstrate that approximately 80% of the evaluated study sample exhibited cognitive impairment. Through the multivariate regression models, we show that several factors, including age, education, depression levels, and the use of analgesics and/or opioids, are significant predictors of total cognitive scores. These factors are independent of the clinical features associated with SCI, such as age, sex, education, and time since the injury. CONCLUSIONS: The results indicate a high prevalence of significant cognitive impairment within the sample, with age, education, depression levels, and the use of analgesics and/or opioids emerging as the primary predictors of total cognitive scores, independent of the clinical features correlated to SCI. These findings hold significant implications for both clinical research and practice, offering valuable guidance for comprehensive management throughout hospitalization and rehabilitation.


Assuntos
Disfunção Cognitiva , Traumatismos da Medula Espinal , Humanos , Traumatismos da Medula Espinal/complicações , Traumatismos da Medula Espinal/epidemiologia , Traumatismos da Medula Espinal/psicologia , Masculino , Feminino , Pessoa de Meia-Idade , Adulto , Disfunção Cognitiva/etiologia , Disfunção Cognitiva/epidemiologia , Disfunção Cognitiva/diagnóstico , Estudos Transversais , Brasil/epidemiologia , Prevalência , Adulto Jovem , Idoso , Fatores Etários , Depressão/epidemiologia , Depressão/etiologia , Depressão/diagnóstico , Escolaridade
19.
Sleep Med ; 117: 25-32, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38503197

RESUMO

OBJECTIVE: The present study assessed the influence of physical training on cardiac autonomic activity in individuals with spinal cord injury (SCI) during different sleep stages. METHODS: Twenty-six volunteers were allocated into three groups: 9 sedentary individuals without SCI (control, CON); 8 sedentary tetraplegic individuals with chronic SCI (SED-SCI); 9 physically trained tetraplegic individuals with chronic SCI (TR-SCI). All participants underwent nocturnal polysomnography to monitor sleep stages: wakefulness, non-rapid eye movement (NREM) sleep (N1, N2, and N3 stages), and REM sleep. The electrocardiography data obtained during this exam were extracted to analyze the heart rate variability (HRV). RESULTS: Sleep stages influenced HRV in the time [RR interval and root mean square of successive RR interval differences (RMSSD)] and frequency [low-frequency (LF) and high-frequency (HF) powers and LF-to-HF ratio] domains (P < 0.05). SED-SCI individuals showed unchanged HRV compared to CON (P > 0.05). When comparing the TR-SCI and SED-SCI groups, no significant differences in HRV were reported in the time domain (P > 0.05). However, in the frequency domain, more accentuated HF power was observed in TR-SCI than in SED-SCI individuals during the N2 and N3 stages and REM sleep (P < 0.05). Moreover, TR-SCI had higher HF power than CON during the N3 stage (P < 0.05). CONCLUSIONS: TR-SCI individuals have greater HF power, indicative of parasympathetic modulation, than sedentary (injured or not injured) individuals during different sleep stages. Therefore, enhanced parasympathetic activity induced by physical training may improve cardiac autonomic modulation during sleep in individuals with chronic SCI.


Assuntos
Fases do Sono , Traumatismos da Medula Espinal , Humanos , Fases do Sono/fisiologia , Sistema Nervoso Autônomo , Sono/fisiologia , Traumatismos da Medula Espinal/complicações , Sono REM/fisiologia , Frequência Cardíaca/fisiologia
20.
J Clin Sleep Med ; 20(7): 1119-1129, 2024 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-38420961

RESUMO

STUDY OBJECTIVES: The objective of this study was to discern distinguishing characteristics of sleep-related breathing disorders in individuals with chronic spinal cord injury (CSCI) compared with participants without CSCI. Additionally, the study investigated factors associated with sleep-related breathing disorder severity. METHODS: This is a cross-sectional analysis of 123 individuals without CSCI, 40 tetraplegics, and 48 paraplegics who underwent attended or partially supervised full polysomnography for suspected sleep-related breathing disorders in a rehabilitation center. Polysomnographic, transcutaneous capnography, and clinical data were collected and compared between the groups. RESULTS: Among tetraplegics, apnea-hypopnea index ≥ 30 events/h (67.5%, P = .003), central apnea (17.5%, P = .007), and higher oxygen desaturation index (80.0%, P = .01) prevailed. Sleep-related hypoventilation was present in 15.4% of tetraplegics and 15.8% of paraplegics, compared with 3.2% in participants without CSCI (P = .05). In the group without CSCI and the paraplegic group, snoring and neck circumference were positively correlated with obstructive sleep apnea (OSA) severity. A positive correlation between waist circumference and OSA severity was identified in all groups, and multivariate logistic regression analysis showed that loud snoring and waist circumference had the greatest impact on OSA severity. CONCLUSIONS: Severe OSA and central sleep apnea prevailed in tetraplegic participants. Sleep-related hypoventilation was more common in tetraplegics and paraplegics than in participants without CSCI. Loud snoring and waist circumference had an impact on OSA severity in all groups. We recommend the routine implementation of transcutaneous capnography in individuals with CSCI. We underscore the significance of conducting a comprehensive sleep assessment in the rehabilitation process for individuals with CSCI. CITATION: Souza Bastos P, Amaral TLD, Yehia HC, Tavares A. Prevalences of sleep-related breathing disorders and severity factors in chronic spinal cord injury and abled-bodied individuals undergoing rehabilitation: a comparative study. J Clin Sleep Med. 2024;20(7):1119-1129.


Assuntos
Polissonografia , Índice de Gravidade de Doença , Síndromes da Apneia do Sono , Traumatismos da Medula Espinal , Humanos , Traumatismos da Medula Espinal/complicações , Traumatismos da Medula Espinal/reabilitação , Traumatismos da Medula Espinal/fisiopatologia , Traumatismos da Medula Espinal/epidemiologia , Masculino , Feminino , Estudos Transversais , Adulto , Síndromes da Apneia do Sono/epidemiologia , Síndromes da Apneia do Sono/complicações , Prevalência , Pessoa de Meia-Idade , Doença Crônica , Paraplegia/complicações , Paraplegia/epidemiologia , Quadriplegia/complicações , Quadriplegia/epidemiologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA