Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 40.943
Filtrar
1.
Orv Hetil ; 163(2): 53-62, 2022 01 09.
Artigo em Húngaro | MEDLINE | ID: mdl-34999571

RESUMO

Összefoglaló. A landiolol intravénásan alkalmazandó, kifejezetten cardioselectiv, gyors hatású és rövid felezési ideju béta-1-receptor-blokkoló, mely elsosorban negatív chronotrop és inotrop hatással bír, vérnyomáscsökkento hatása elhanyagolható. Foleg hemodinamikailag instabil állapotú, supraventricularis ritmuszavarban szenvedo betegek kamrafrekvenciájának csökkentésére használható. Nagy esetszámú, randomizált vizsgálatok igazolták hatékonyságát szívmutétek után jelentkezo pitvarfibrilláció megelozésében, valamint súlyos akut szívelégtelenségben és szívmutétek posztoperatív szakában jelentkezo pitvari tachyarrhythmiák kezelésében. Ezek mellett kisebb vizsgálatok alapján a használata biztonságosnak tunik akut myocardialis infarctusban, hatékony szeptikus állapotú, pitvarfibrillációban szenvedo betegek kamrafrekvencia- és ritmuskontrolljára, valamint nem cardialis mutétek esetén a pitvarfibrilláció prevenciójára és kezelésére. Sikerrel alkalmazható elektromos vihar esetén is, és jól használható angiográfiás coronaria-CT-vizsgálat elott az optimális szívfrekvencia elérésére. A gyógyszer 2016 óta Európában, 2018 óta Magyarországon is elérheto. Orv Hetil. 2022; 163(2): 53-62. Summary. Landiolol is an intravenous, selective beta-1-receptor blocking agent with rapid onset of action and ultra-short half-life that has a predominant negative chronotropic and only mild negative inotropic effect without significant reduction of blood pressure. Landiolol is indicated to control the ventricular heart rate predominantly in patients with hemodynamic instability due to supraventricular tachyarrhythmia. Large randomized controlled trials have proven the efficacy of landiolol in the prevention of atrial fibrillation and atrial tachyarrhythmias in severe acute heart failure or post-cardiac surgery. Based on lower case-number studies, the administration of landiolol has been proven to be efficient and safe in rhythm and rate control in atrial fibrillation complicating acute myocardial infarction, sepsis, and in the prevention of atrial fibrillation in non-cardiac surgery. Landiolol may be used in electrical storm, and even during coronary CT-angiography to achieve an optimal heart rate for imaging. The drug is available in Europe since 2016 and in Hungary since 2018. Orv Hetil. 2022; 163(2): 53-62.


Assuntos
Fibrilação Atrial , Infarto do Miocárdio , Cuidados Críticos , Frequência Cardíaca , Humanos , Morfolinas , Ureia/análogos & derivados
2.
Clin Lab ; 68(1)2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-35023679

RESUMO

BACKGROUND: Phenylketonuria (PKU) is the most common aminoacidopathy with an autosomal recessive inheritance pattern. A global PKU prevalence is estimated about 6.002 in 100,000 newborns. In Iran, the prevalence of PKU is estimated at about 1 in 4,698, and it shows an increasing trend from north (0.0015%) to south (0.02%) of the country. Untreated PKU causes mental retardation, microcephaly, and seizure. PAH gene mutations located at chromosome 12q23 are responsible for the classical type of this disease. The spectrum of PAH mutations is varied in different ethnicities and different parts of the world. The aim of this study was to investigate the frequency of PAH mutation in the Mazandaran province, which could be useful for genetic counseling and prenatal diagnosis. METHODS: A total of 66 individuals from 33 families from two provinces (9 families from Golestan and 24 families from Mazandaran) from north of Iran participated in this study. After genomic DNA extraction, PAH gene analysis was carried out using DNA sequencing of both coding and non-coding regions by ABI 3130XL genetic analyzer. RESULTS: Twenty-six different mutations were identified in the PAH gene in this study. Four mutations including IVS10-11 (c.1066-11G>A), c.727C>T (p.Arg243X), c.898G>T (p.Ala300Ser), and c.601C>T (p.His201Tyr) were the most common mutations with 37.48% frequency in Mazandaran province. Most frequent mutations in Golestan province were IVSI0-11 (c.1066-11G>A), c.722delG (p.Arg241fs), c.842C>T (p.Pro281Leu), and IVSII+5 (G>A) with frequency 58.57%. CONCLUSIONS: The results from the present study verify heterogeneity of the PAH gene and may help to diagnose tests for carrier detection and prenatal diagnosis of the PKU disease in Iranian population.


Assuntos
Fenilalanina Hidroxilase , Fenilcetonúrias , Frequência do Gene , Humanos , Recém-Nascido , Irã (Geográfico)/epidemiologia , Mutação , Fenilalanina Hidroxilase/genética , Fenilcetonúrias/diagnóstico , Fenilcetonúrias/epidemiologia , Fenilcetonúrias/genética , Ureia
3.
Anal Chim Acta ; 1191: 338891, 2022 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-35033244

RESUMO

In this study, we present a simple method to determine imidazolidinyl urea (IU) in cosmetics using a solid phase as both a decomposition field and an extraction phase. IU is difficult to quantify because it is a mixture of allantoin-formaldehyde condensation products that are easily decomposed to release formaldehyde. In our method, IU is decomposed to allantoin and 1-[4-(hydroxymethyl)-2,5-dioxoimidazolidin-4-yl]urea (4-HU) on an aminopropyl-bonded silica solid phase. Subsequent high-performance liquid chromatography enables quantification of the resulting allantoin and 4-HU. The quantified value was converted to the total allantoin amount on the basis of molecular weight, and the calculated value was compared with that of an IU reference standard to determine the contents. The calibration curves of the decomposed IU as allantoin and 4-HU were both linear over an IU solution concentration range from 0.05 to 0.65% (w/v). The recoveries from lotion, body soap, and conditioners, which contained 0.1%, 0.3%, and 0.6% (w/w) of IU, respectively, ranged from 88.2 to 107.5%. The relative standard deviation values for the recovery tests of six replicates ranged from 1.03 to 6.97%. The intra-laboratory precisions for the lotion and conditioner A containing 0.3% IU were 3.02 and 4.94%, respectively. This method was well validated and would be helpful in determining IU in cosmetic samples.


Assuntos
Cosméticos , Cromatografia Líquida de Alta Pressão , Cosméticos/análise , Formaldeído , Ureia/análogos & derivados
4.
Environ Pollut ; 292(Pt A): 118301, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34626716

RESUMO

Almost 81% of nitrogen fertilizers are applied in form of urea but most of it is lost due to volatilization and leaching leading to environmental pollution. In this regard, slow-release nano fertilizers can be an effective solution. Here, we have synthesized different Fe3O4-urea nanocomposites with Fe3O4 NPs: urea ratio (1:1, 1:2, 1:3) ie. NC-1, 2, and 3 respectively, and checked their efficacy for growth and yield enhancement. Oryza sativa L. cv. Swarna seedlings were treated with different NCs for 14 days in hydroponic conditions and significant up-regulation of photosynthetic efficiency and nitrogen metabolism were observed due to increased availability of nitrogen and iron. The discriminant functional analysis confirmed that the NC3 treatment yielded the best results so further gene expression studies were performed for NC-3 treated seedlings. Significant changes in expression profiles of ammonia and nitrate transporters indicated that NC-3 treatment enhanced nitrogen utilization efficiency (NUE) due to sustained slow release of urea. From pot experiments, we found significant enhancement of growth, grain nutrient content, and NUE in NC supplemented sets. 1.45 fold increase in crop yield was achieved when 50% N was supplemented in form of NC-3 and the rest in form of ammonium nitrate. NC supplementation can also play a vital role in minimizing the use of bulk N fertilizers because, when 75% of the recommended N dose was supplied in form of NC-3, 1.18 fold yield enhancement was found. Thus our results highlight that, slow-release NC-3 can play a major role in increasing the NUE of rice.


Assuntos
Nanocompostos , Oryza , Agricultura , Poluição Ambiental , Fertilizantes/análise , Nitrogênio/análise , Nutrientes , Solo , Ureia
5.
Bioresour Technol ; 344(Pt B): 126301, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34752883

RESUMO

To explore the effectiveness of urea replacing poultry manure as the nitrogen source in the rice husk composting system, and to promote the utilization of compost products as substrates, 0%, 10%, 20%, and 30% of phosphogypsum were added respectively in the urea composting system, and were compared with the chicken manure composting (RCP0). Finally, the fermentation and maturation of RCP0 were achieved, but high EC value limited the utilization of compost products as the substrate. Urea, as an N source, could lower the EC value, but the C/N ratio was uncoordinated during the initial stage of composting. Adding an appropriate proportion of phosphogypsum could ensure a proper C/N ratio to promote smooth fermentation and enable the products to be ideal substrates. When the added proportion was 30%, the thermophilic stage was shortened significantly but this may increase heavy metals. 10%-20% were concluded to be the recommended proportion.


Assuntos
Compostagem , Oryza , Animais , Sulfato de Cálcio , Esterco , Nitrogênio/análise , Fósforo , Solo , Ureia
6.
Chemosphere ; 288(Pt 1): 132459, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34619254

RESUMO

The demand for ultrapure water (UPW) in the semiconductor industry has increased in recent years, while the idea to use reclaimed water instead of tap water for UPW production has also attracted more attention. However, since urea concentration in reclaimed water is higher than that in tap water, UPW production has not been efficient. To resolve this problem, this study aims to develop a new spent coffee grounds based biochar (SCG-BC)/persulfate catalytic system as a pretreatment unit. The objective is to enhance urea removal from reclaimed water so that UPW production is more effective. In this study, the biochar used was prepared from spent coffee grounds with detailed characterization. Results strongly suggested that the urea removed by SCG-BC/persulfate catalytic system was very encouraging (up to 73%). The best possible dosages for SCG-BC and persulfate for urea removal were 0.2 and 2.0 g L-1, respectively. Furthermore, this system could remove urea effectively in a wide range of pH (3-10). Moreover, the characterizations of SCG-BC (graphite C, defective edges and functional groups, i.e. -OH, CO, carboxyl C-O) helped to activate persulfate in the catalytic process. OH• and SO4• - were all involved in this process, while the SO4• - was the main radical for urea degradation.


Assuntos
Café , Poluentes Químicos da Água , Carvão Vegetal , Ureia , Água , Poluentes Químicos da Água/análise
7.
Int Clin Psychopharmacol ; 37(1): 25-28, 2022 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-34686643

RESUMO

Lithium, a mood stabilizer used in the treatment of bipolar disorder is known for its anti-inflammatory properties with the discussion of its potential use in COVID-19 infection. The SARS-CoV-2 virus causing COVID-19 infection is known to enter the target cells through angiotensin converting enzyme-2 receptors present in abundance in the lung and renal tissue. Recent research supports the evidence for direct renal injury by viral proteins. Here we report two patients with bipolar disorder presenting with lithium toxicity in the presence of COVID-19 infection. Two patients with bipolar disorder, maintaining remission on lithium prophylaxis, presented to the psychiatric emergency with recent-onset fever and altered sensorium. Both the patient's investigations revealed lithium toxicity, elevated serum creatinine, urea and inflammatory markers. Hypernatremia, hyperkalaemia, and hyperchloremia were seen in one patient. Lithium and other psychotropic medications were stopped immediately, and COVID-19 treatment was initiated. Patient with clinical signs of lithium toxicity, hypernatremia, hyperkalaemia, and hyperchloremia developed ventricular tachycardia. He survived and regained consciousness after 2 weeks of aggressive conservative management. However, another patient died of acute respiratory failure on day 3. Possible direct infection of the kidney by SARS-CoV-2 viral proteins can manifest with acute kidney injury and lithium toxicity among patients on long-term lithium therapy. Health professionals treating COVID-19 infection among individuals on lithium therapy should be aware of the possibility of lithium toxicity in the background of renal injury.


Assuntos
Anti-Inflamatórios não Esteroides/efeitos adversos , Antimaníacos/efeitos adversos , COVID-19/complicações , Compostos de Lítio/efeitos adversos , Antimaníacos/uso terapêutico , Transtorno Bipolar/complicações , Transtorno Bipolar/tratamento farmacológico , Creatinina/sangue , Evolução Fatal , Humanos , Hiperpotassemia/induzido quimicamente , Hipernatremia/induzido quimicamente , Compostos de Lítio/uso terapêutico , Masculino , Pessoa de Meia-Idade , Insuficiência Respiratória/induzido quimicamente , Taquicardia Ventricular/induzido quimicamente , Ureia/sangue
8.
J Colloid Interface Sci ; 607(Pt 2): 1849-1863, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34688976

RESUMO

Infected wounds show delayed and incomplete healing processes and even render patients at a high risk of death due to the formed bacterial biofilms in the wound site, which protect bacteria against antimicrobial treatments and immune response. Nitric oxide based therapy is considered a promising strategy for eliminating biofilms and enhancing wound healing, which encounters a significant challenge of controlling the NO release behavior at the wound site. Herein, a kind of phenylalanine based poly(ester urea)s with high thermal stability are synthesized and fabricated to electrospun films as NO loading vehicle for infected wound treatment. The resultant films can continuously and stably release nitric oxide for 360 h with a total concentration of 1.15 µmol L-1, which presents obvious advantages in killing the bacteria and removing biofilms. The results exhibit the films have no cytotoxicity and may accelerate the wound repair without causing inflammation, hemolysis, or cytotoxic reactions as well as stimulate the proliferation of fibroblasts and increase the synthesis of collagen. Therefore, the films may be a suitable NO releasing dressing for removing biofilms and repairing infected wounds.


Assuntos
Ésteres , Óxido Nítrico , Antibacterianos , Biofilmes , Humanos , Fenilalanina , Ureia , Cicatrização
9.
Food Chem ; 369: 130882, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34481403

RESUMO

Starch nanoparticles (SNPs) with different morphology and particle size can be prepared by modulating the reaction conditions over SNPs preparation. This study was to synthesize various SNPs by using ultrasound assisted nanoprecipitation method, and characterized by particle size analysis, SEM and XRD performing. SNPs were successfully produced via nanoprecipitation and the particle size were controlled in the range of 95 to 150 nm. Moreover, variously different morphologies were obtained when using corn, potato or Trichosanthes kirilowii pulp (TKP) starch to produce nanoparticles, including fiber, flake and film. Results shown film TKP SNPs demonstrated an improved urea adsorption rate to 135.60 mg/g with the highest qm at 1.00 mg/mL. SNPs can be developed using ultrasound assisted nanoprecipitation method and the particle size together with surface morphology can be varied according to the source of starch and preparation method, while surface morphology is the key factor in altering adsorption performance.


Assuntos
Nanopartículas , Amido , Adsorção , Tamanho da Partícula , Ureia
10.
Sci Total Environ ; 803: 149902, 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-34482144

RESUMO

Nitrogen (N) use efficiency can be increased by the addition of substances to urea. Magnesium sulfate (MgSO4) and boron were considered as plant nutrients, while zeolite was used as soil conditioner. The addition of these substances may affect soil NH3 and N2O emissions, by increasing N use efficiency. We conducted an 30 days incubation experiment with ryegrass using fertilizer treatments (12 g N m-2) as follows: urea (U); urea + MgSO4 (UM); urea + MgSO4 + borax (UMB); zeolite + urea + MgSO4 (Z-UM); and zeolite + urea + MgSO4 + borax (Z-UMB). We measured NH3 and N2O emissions and the aboveground N uptake of ryegrass. Cumulative NH3 emissions of UM, UMB, Z-UM and Z-UMB were 10%, 53%, 21% and 58% lower than U, respectively, while their N2O emissions were 32%, 133%, 43% and 72% higher than U, respectively. Aboveground N uptake of UM, UMB, Z-UM and Z-UMB were 9%, 6%, 12% and 13% higher than U, respectively. Overall, we suggest that the addition of MgSO4 and borax were effective in reducing NH3 emissions and potentially increase plant N uptake. However, the risk of higher denitrification and N2O emissions also needs to be considered. This study reveals the considerable effect of MgSO4 and borax in soil N cycles. Future research should evaluate how the application of urea + MgSO4 + borax effects gaseous emissions and crop yield of dicotyledons and in drier soil conditions.


Assuntos
Solo , Ureia , Agricultura , Boratos , Fertilizantes/análise , Sulfato de Magnésio , Nitrogênio/análise , Óxido Nitroso/análise , Poaceae
11.
Spectrochim Acta A Mol Biomol Spectrosc ; 265: 120353, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34492514

RESUMO

The catalytic amplification signal of polystyrene nanosphere (PN) is used to conveniently fabricate the resonance Rayleigh scattering (RRS)/surface-enhanced Raman scattering (SERS) dual-mode method to sensitively and selectively detect urea in food. PN has strong catalysis of the slow nanoreaction of citrate-Ag(I) to produce yellow silver nanoparticles (AgNP), which exhibit strong RRS effect and SERS effect with molecular probes. When aptamer (Apt) is present, the Apt is adsorbed on the PN surface, the catalysis is weakened, the AgNP is reduced, and the SERS/RRS signal is weakened. After adding urea to exhibit specific Aptamer reaction, the Apt is desorbed from the PN surface and the catalysis is restored. As urea increase, the desorbed PNs increase to produce more AgNPs indicator to increase SERS/RRS signal. The increase value △I of SERS/RRS is linearly to urea concentration. Therefore, a sensitive and selective SERS/RRS dual-mode method for urea is established based on aptamers-regulated the catalysis of PNs. This method is applied to the detection of urea in milk with satisfactory results. The relative standard deviation is 3.9-6.8% and the recovery rate is 94.5-102%.


Assuntos
Nanopartículas Metálicas , Nanosferas , Catálise , Poliestirenos , Prata , Ureia
12.
Sci Total Environ ; 805: 150339, 2022 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-34537697

RESUMO

Biochar derived from nitrogen-rich pyrolysis of biomass can be used as a soil conditioner, but it contains a large amount of environmental persistent free radicals (EPFRs). EPFRs are a newly identified environmentally harmful substance, and the detection and research on EPFRs in nitrogen-rich pyrolyzed char is lacking. Biochars prepared from cellulose-urea mixtures at different temperatures, residence times, and urea ratios were analyzed in this study. EPFRs in biochar prepared at 500 °C had the highest spin concentrations. Substituted aromatic compounds were the precursors to the EPFRs. The types of EPFRs in biochars shifted from oxygen-centered at 400 °C to carbon- and oxygen-centered in the 450-600 °C range due to a reduction in oxygen-containing functional groups. Residence time experiments showed that most EPFRs formed in the first 5 min of pyrolysis. C was the main element used for the formation of EPFRs, while N content was negatively correlated with the concentration of EPFRs. Pyrolysis temperature was the key factor determining the types of EPFRs produced, while proportion of urea only affected the concentrations of EPFRs and not type. The results of this study are of great significance for understanding the environmental behavior of common EPFRs in nitrogen-rich biochar.


Assuntos
Pirólise , Ureia , Celulose , Carvão Vegetal , Radicais Livres , Temperatura
13.
J Colloid Interface Sci ; 607(Pt 2): 1014-1022, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34571291

RESUMO

HYPOTHESIS: The unexpected formation of a lamellar structure with concomitant gelation in solutions containing high urea concentration (40 wt%) and relatively low amount of cationic surfactant (3 wt%), indicates that a hierarchically structured complex is formed by both molecules. EXPERIMENTS: Gels formed by combination of aqueous solutions of urea and C12TAB, C14TAB or C16TAB were prepared in different proportions and their structures at microscopic and mesoscopic levels were investigated using XRD and SAXS, respectively. The elastic and viscous moduli and yield stress of the samples were determined and correlated with the composition and structuration of the gels. The lamellar structure is reversibly thermically destroyed and this process was investigated using DSC. FINDINGS: XRD revealed that, at microscopic scale, the gels are formed through crystallization of adducts containing surfactant molecules loaded into the cavities of honeycomb-like urea assemblies. Such crystalline phase arranges itself in lamellae with interplanar distance around ∼20-30 nm, which were observed by SAXS. This hierarchical structure is independent of the chain length of the cationic surfactants. The blocks of lamellae dispersed in the continuous phase form a three-dimensional rigid particulate network structure, giving the characteristic rheological behavior of a hydrogel. DSC revealed a reversible thermal transition at around 20-25 °C, beyond which the adducts and the lamellar phase are destroyed and micelles are formed. The characteristic transition temperature is independent of the chain length of the surfactant, and thus, it is not associated with their Krafft temperatures. The structures of the gels indicate that they resemble alpha-gels formed by fatty-alcohols and surfactants, although they self-assemble by different driving forces.


Assuntos
Tensoativos , Ureia , Géis , Espalhamento a Baixo Ângulo , Difração de Raios X
14.
Bioresour Technol ; 343: 126062, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34601025

RESUMO

This study evaluated the performance of spent coffee biochar (SCBC)/granular activated carbon (GAC) activating peroxymonosulfate (PMS) and peroxydisulfate (PDS) for urea degradation in reclaimed water used for ultrapure water production. Results showed that catalyst and oxidant wielded a great influence on urea removal. Of them, the GAC-PMS system could completely remove urea at the least oxidant (1 g/L) and catalyst dosage (0.2 g/L). GAC activating PMS mainly depended on graphite C structure and minor oxygen functional groups. However, the amounts of urea removed by 600BC-PMS and 900BC-PMS were 57% and 70%, respectively. In the PDS system, the urea removal through GAC-PDS could reach 90%, which mainly depends on the graphite C structure of GAC. Using the same conditions, the urea removal of 900BC-PDS was similar to GAC-PDS, so it has some potential as an alternative to commercial GAC.


Assuntos
Carvão Vegetal , Poluentes Químicos da Água , Café , Peróxidos , Ureia , Água , Poluentes Químicos da Água/análise
15.
Ying Yong Sheng Tai Xue Bao ; 32(12): 4419-4428, 2021 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-34951283

RESUMO

We carried out pot experiment to investigate nitrogen transformation characteristics, yield increasing effect, and apparent utilization rate of nitrogen fertilizer in loess soils by combining chemi-cal inhibitor and biostimulant humic acid to investigate the application effect and provide a theoretical basis for new type highly efficient and stable urea in loess soil. In this study, blank (CK) and urea (N) were set as controls, and humic acid alone (F), N-butyl thiophosphate-triamine (NBPT), 3,4-dimethyl-pyrazolate phosphate (DMPP), 2-chloro-6-trimethyl-pyridine (CP) and humic acid were respectively combined with three biochemical inhibitors to urea. The results showed that compared with N treatment, F, NBPT+F, DMPP+F, CP+F treatments significantly increased maize yield, chlorophyll content, leaf area index and nitrogen uptake, and had obvious effects on soil available nitrogen contents. The addition of humic acid increased chlorophyll content of maize leaves in all cases compared to the application of biochemical inhibitors alone. Compared with CP treatment, CP+F treatment could significantly increase nitrogen uptake, chlorophyll content, and nitrogen adsorption efficiency of maize. Addition of humic acid with NBPT increased nitrification inhibition rate by 10.7% compared with NBPT alone, but decreased yield, leaf area index, nitrogen uptake, nitrogen use efficiency. Compared with DMPP treatment, DMPP+F treatment significantly reduced maize yield, leaf area index, nitrogen uptake, nitrogen use efficiency and nitrification inhibition rate. Considering maize yield, plant N uptake, N fertilizer uptake rate and soil ammonium N and nitrate N contents, the addition of humic acid and CP is recommended for urea application in loess areas to enhance urea performance, yield, and fertilizer utilization.


Assuntos
Substâncias Húmicas , Ureia , Fertilizantes/análise , Nitrificação , Nitrogênio/análise , Solo
16.
Nutrients ; 13(12)2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34959754

RESUMO

This study evaluates the capacity of a bread enriched with fermentable dietary fibres to modulate the metabolism and nutrients handling between tissues, gut and peripheral, in a context of overfeeding. Net fluxes of glucose, lactate, urea, short chain fatty acids (SCFA), and amino acids were recorded in control and overfed female mini-pigs supplemented or not with fibre-enriched bread. SCFA in fecal water and gene expressions, but not protein levels or metabolic fluxes, were measured in muscle, adipose tissue, and intestine. Fibre supplementation increased the potential for fatty acid oxidation and mitochondrial activity in muscle (acox, ucp2, sdha and cpt1-m, p < 0.05) as well as main regulatory transcription factors of metabolic activity such as pparα, pgc-1α and nrf2. All these features were associated with a reduced muscle fibre cross sectional area, resembling to controls (i.e., lean phenotype). SCFA may be direct inducers of these cross-talk alterations, as their feces content (+52%, p = 0.05) was increased in fibre-supplemented mini-pigs. The SCFA effects could be mediated at the gut level by an increased production of incretins (increased gcg mRNA, p < 0.05) and an up-regulation of SCFA receptors (increased gpr41 mRNA, p < 0.01). Hence, consumption of supplemented bread with fermentable fibres can be an appropriate strategy to activate muscle energy catabolism and limit the establishment of an obese phenotype.


Assuntos
Tecido Adiposo/metabolismo , Fibras na Dieta/administração & dosagem , Metabolismo Energético/efeitos dos fármacos , Músculo Esquelético/metabolismo , Hipernutrição/metabolismo , Aminoácidos/metabolismo , Animais , Pão , Suplementos Nutricionais , Modelos Animais de Doenças , Ácidos Graxos Voláteis/metabolismo , Fezes/química , Feminino , Glucose/metabolismo , Incretinas/metabolismo , Intestinos/metabolismo , Ácido Láctico/metabolismo , Suínos , Porco Miniatura , Ureia/metabolismo
17.
Cell Mol Life Sci ; 79(1): 41, 2021 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-34913090

RESUMO

Living organisms repeatedly encounter stressful events and apply various strategies to survive. Polyamines are omnipresent bioactive molecules with multiple functions. Their transient synthesis, inducible by numerous stressful stimuli, is termed the polyamine stress response. Animals developed evolutionarily conserved strategies to cope with stresses. The urea cycle is an ancient attribute that deals with ammonia excess in terrestrial species. Remarkably, most fish retain the urea cycle genes fully expressed during the early stages of development and silenced in adult animals. Environmental challenges instigate urea synthesis in fish despite substantial energetic costs, which poses the question of the urea cycle's evolutionary significance. Arginase plays a critical role in oxidative stress-dependent reactions being the final urea cycle enzyme. Its unique subcellular localization, high inducibility, and several regulation levels provide a supreme ability to control the polyamine synthesis rate. Notably, oxidative stress instigates the arginase-1 activity in mammals. Arginase is also dysregulated in aging organisms' brain and muscle tissues, indicating its role in the pathogenesis of age-associated diseases. We designed a study to investigate the levels of the urea cycle and polyamine synthesis-related enzymes in a fish model of acute hypoxia. We evidence synchronized elevation of arginase-2 and ornithine decarboxylase following oxidative stress in adult fish and aging animals signifying the specific function of arginase-2 in fish. Moreover, we demonstrate oxidative stress-associated polyamine synthesis' induction and urea cycle' arrest in adult fish. The subcellular arginase localization found in the fish seems to correspond to its possible evolutionary roles.


Assuntos
Envelhecimento/metabolismo , Arginase/metabolismo , Hipóxia/metabolismo , Estresse Oxidativo , Ureia/metabolismo , Doença Aguda , Animais , Ornitina Descarboxilase/metabolismo , Peixe-Zebra
18.
Biomolecules ; 11(12)2021 11 24.
Artigo em Inglês | MEDLINE | ID: mdl-34944403

RESUMO

The pyrazolyl-urea Gege3 molecule has shown interesting antiangiogenic effects in the tumor contest. Here, we have studied the role of this compound as interfering with endothelial cells activation in response to the paracrine effects of annexin A1 (ANXA1), known to be involved in promoting tumor progression. ANXA1 has been analyzed in the extracellular environment once secreted through microvesicles (EVs) by pancreatic cancer (PC) cells. Particularly, Gege3 has been able to notably prevent the effects of Ac2-26, the ANXA1 mimetic peptide, and of PC-derived EVs on endothelial cells motility, angiogenesis, and calcium release. Furthermore, this compound also inhibited the translocation of ANXA1 to the plasma membrane, otherwise induced by the same ANXA1-dependent extracellular stimuli. Moreover, these effects have been mediated by the indirect inhibition of protein kinase Cα (PKCα), which generally promotes the phosphorylation of ANXA1 on serine 27. Indeed, by the subtraction of intracellular calcium levels, the pathway triggered by PKCα underwent a strong inhibition leading to the following impediment to the ANXA1 localization at the plasma membrane, as revealed by confocal and cytofluorimetry analysis. Thus, Gege3 appeared an attractive molecule able to prevent the paracrine effects of PC cells deriving ANXA1 in the tumor microenvironment.


Assuntos
Anexina A1/metabolismo , Regulação para Baixo , Vesículas Extracelulares/metabolismo , Neoplasias Pancreáticas/metabolismo , Bibliotecas de Moléculas Pequenas/farmacologia , Ureia/química , Anexina A1/farmacologia , Cálcio/metabolismo , Linhagem Celular Tumoral , Membrana Celular/metabolismo , Movimento Celular/efeitos dos fármacos , Vesículas Extracelulares/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Células Endoteliais da Veia Umbilical Humana , Humanos , Neoplasias Pancreáticas/tratamento farmacológico , Comunicação Parácrina/efeitos dos fármacos , Peptídeos/farmacologia , Fosforilação/efeitos dos fármacos , Transporte Proteico/efeitos dos fármacos , Bibliotecas de Moléculas Pequenas/química , Microambiente Tumoral/efeitos dos fármacos
19.
PLoS One ; 16(12): e0260528, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34937056

RESUMO

Electrogenic bacteria produce power in soil based terrestrial microbial fuel cells (tMFCs) by growing on electrodes and transferring electrons released from the breakdown of substrates. The direction and magnitude of voltage production is hypothesized to be dependent on the available substrates. A sensor technology was developed for compounds indicative of anthropological activity by exposing tMFCs to gasoline, petroleum, 2,4-dinitrotoluene, fertilizer, and urea. A machine learning classifier was trained to identify compounds based on the voltage patterns. After 5 to 10 days, the mean voltage stabilized (+/- 0.5 mV). After the entire incubation, voltage ranged from -59.1 mV to 631.8 mV, with the tMFCs containing urea and gasoline producing the highest (624 mV) and lowest (-9 mV) average voltage, respectively. The machine learning algorithm effectively discerned between gasoline, urea, and fertilizer with greater than 94% accuracy, demonstrating that this technology could be successfully operated as an environmental sensor for change detection.


Assuntos
Fontes de Energia Bioelétrica/microbiologia , Técnicas Biossensoriais/métodos , Fertilizantes/análise , Gasolina/análise , Aprendizado de Máquina , Microbiologia do Solo , Ureia/análise
20.
Biomolecules ; 11(12)2021 12 15.
Artigo em Inglês | MEDLINE | ID: mdl-34944532

RESUMO

It has been considered that proline dehydrogenase/proline oxidase (PRODH/POX) is involved in antineoplastic activity of metformin (MET). The aim of this study is identification of key metabolites of glycolysis, pentose phosphate pathway (PPP), tricarboxylic acids (TCA), urea cycles (UC) and some amino acids in MET-treated MCF-7 cells and PRODH/POX-knocked out MCF-7 (MCF-7crPOX) cells. MCF-7crPOX cells were generated by using CRISPR-Cas9. Targeted metabolomics was performed by LC-MS/MS/QqQ. Expression of pro-apoptotic proteins was evaluated by Western blot. In the absence of glutamine, MET treatment or PRODH/POX-knock out of MCF-7 cells contributed to similar inhibition of glycolysis (drastic increase in intracellular glucose and pyruvate) and increase in the utilization of phospho-enol-pyruvic acid, glucose-6-phosphate and some metabolites of TCA and UC, contributing to apoptosis. However, in the presence of glutamine, MET treatment or PRODH/POX-knock out of MCF-7 cells contributed to utilization of some studied metabolites (except glucose), facilitating pro-survival phenotype of MCF-7 cells in these conditions. It suggests that MET treatment or PRODH/POX-knock out induce similar metabolic effects (glucose starvation) and glycolysis is tightly linked to glutamine metabolism in MCF-7 breast cancer cells. The data provide insight into mechanism of anticancer activity of MET as an approach to further studies on experimental breast cancer therapy.


Assuntos
Neoplasias da Mama/metabolismo , Glutamina/metabolismo , Metabolômica/métodos , Metformina/farmacologia , Prolina Oxidase/genética , Apoptose , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/genética , Cromatografia Líquida , Feminino , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Técnicas de Inativação de Genes , Glicólise/efeitos dos fármacos , Humanos , Células MCF-7 , Via de Pentose Fosfato/efeitos dos fármacos , Espectrometria de Massas em Tandem , Ácidos Tricarboxílicos/metabolismo , Ureia/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...