Microbiological Evaluation of Commercial Hand Sanitisers Available in Pakistan Using European Standard and Membrane Filtration Method.

OBJECTIVE: To evaluate the antibacterial efficacy of various commercially available alcohol-based hand sanitisers (ABHS) using European standard (EN 1500) method and perform ABHS testing with membrane filtration method. STUDY DESIGN: A Cross-sectional observational study. Place and Duration of the Study: Quality Control Section of the Microbiology Laboratory, The Aga Khan University Hospital, Karachi, Pakistan, from February to April 2023. METHODOLOGY: Efficacy of 14 commercially and widely accessible hand sanitisers was defined as reducing micro-organism growth. It was determined using the EN 1500 European standard test and membrane filtration method. RESULTS: Majority (92.8%) ABHS showed a significant bacterial reduction except one ABHS tested with the EN 1500 method. Only six ABHS products were tested through the membrane filtration method because high viscosity of hand sanitisers was causing damage to filter membranes. CONCLUSION: Continued vigilance in evaluating hand sanitiser's efficacy through robust testing methods is essential to ensure public health and prevent the dissemination of misleading products that may compromise hand hygiene practices. KEY WORDS: Hand sanitisers, European standard, Membrane filtration method, Antibacterial efficacy.

Multifactorial evaluation of an ultra-fast process for electrospinning of recycled expanded polystyrene to manufacture high-efficiency membranes for nanoparticle air filtration.

The increased production of polystyrene waste has led to the need to find efficient ways to dispose of it. One possibility is the use of solid waste to produce filter media by the electrospinning technique. The aim of this work was to develop an ultra-fast electrospinning process applied to recycled polystyrene, with statistical evaluation of the influence of polymeric solution parameters (polymer concentration and percentage of DL-limonene) and process variables (flow rate, voltage, and type of support) on nanoparticle collection efficiency, air permeability, and fiber diameter. An extensive characterization of the materials and evaluation of the morphology of the fibers was also carried out. It was found that recycled expanded polystyrene could be used in electrospinning to produce polymeric membranes. The optimized condition that resulted in the highest nanoparticle collection efficiency was a polymer concentration of 13.5%, percentage of DL-limonene of 50%, voltage of 25 kV, and flow rate of 1.2 mL/h, resulting in values of 99.97 ± 0.01%, 2.6 ± 0.5 × 10-13 m2, 0.19 Pa-1, and 708 ± 176 nm for the collection efficiency of nanoparticles in the range from 6.38 to 232.9 nm, permeability, quality factor, and mean fiber diameter, respectively. All the parameters were found to influence collection efficiency and fiber diameter. The use of DL-limonene, a natural solvent, provided benefits including increased collection efficiency and decreased fiber size. In addition, the electrostatic filtration mechanism was evaluated using the presence of a copper grid as a support for the nanofibers. The findings demonstrated that an electrospinning time of only 5 min was sufficient to obtain filters with high collection efficiencies and low pressure drops, opening perspectives for the application of polystyrene waste in the development of materials with excellent characteristics for application in the area of atmospheric pollution mitigation.

Biofiltration of gaseous mixtures of dimethyl sulfide, dimethyl disulfide and dimethyl trisulfide: Effect of operational conditions and microbial analysis.

The efficient removal of volatile sulfur compounds (VSCs), such as dimethyl sulfide (DMS), dimethyl disulfide (DMDS) and dimethyl trisulfide (DMTS), is crucial due to their foul odor and corrosive potential in sewer systems. Biofilters (BFs) offer promise for VSCs removal, but face challenges related to pH control and changing conditions at full scale. Two BFs, operated under acidophilic conditions for 78 days, were evaluated for their performance at varying inlet concentrations and empty bed residence times (EBRTs). BF1, incorporating 4-6 mm marble limestone for pH control, outperformed BF2, which used NaHCO3 in the nutrient solution. BF1 displayed better resilience, maintained a stable pH of 4.6 ± 0.6, and achieved higher maximum elimination capacities (ECmax, 41 mg DMS m-3 h-1 (RE 38.3%), 146 mg DMDS m-3 h-1 (RE 83.1%), 47 mg DMTS m-3 h-1 (RE 93.1%)) at an EBRT of 56 s compared to BF2 (9 mg DMS m-3 h-1 (RE 7.1%), 9 mg DMDS m-3 h-1 (RE 4.8%) and 11 mg DMTS m-3 h-1 (RE 26.6%)). BF2 exhibited pH stratification and decreased performance after feeding interruptions. The biodegradability of VSCs followed the order DMTS > DMDS > DMS, and several microorganisms were identified contributing to VSCs degradation in BF1, including Bacillus (14%), Mycobacterium (11%), Acidiphilium (7%), and Acidobacterium (3%).

Commercial reverse osmosis point-of-use systems in Egypt failed to purify tap water.

This study addresses the heightened global reliance on point-of-use (PoU) systems driven by water quality concerns, ageing infrastructure, and urbanization. While widely used in Egypt, there is a lack of comprehensive evaluation of these systems. We assessed 10 reverse osmosis point-of-use systems, examining physicochemical, bacteriological, and protozoological aspects of tap water (inlets) and filtered water (outlets), adhering to standard methods for the examination of water and wastewater. Results showed significant reductions in total dissolved solids across most systems, with a decrease from 210 ± 23.6 mg/L in tap water to 21 ± 2.8 mg/L in filtered water for PoU-10. Ammonia nitrogen levels in tap water decreased from 0.05 ± 0.04 to 2.28 ± 1.47 mg/L to 0.02 ± 0.04 to 0.69 ± 0.64 mg/L in filtered water. Despite this, bacterial indicators showed no significant changes, with some systems even increasing coliform levels. Protozoological analysis identified prevalent Acanthamoeba (42.5%), less frequent Naegleria (2.5%), Vermamoeba vermiformis (5%), and potentially pathogenic Acanthamoeba genotypes. Elevated bacterial indicators in filtered water of point-of-use systems, combined with essential mineral removal, indicate non-compliance with water quality standards, posing a public health concern. Further research on the long-term health implications of these filtration systems is essential.

Comparative evaluation of different pre-treatment alternatives for granular media filters treating greywater and their ranking using analytical hierarchy process.

In this study, the performance of four different pre-treatment alternatives for granular media filtration, namely, settling, aeration, coarse media filtration and chemical coagulation were compared experimentally. Further, analytical hierarchy process (AHP) was used to compare their performance based on economic, environmental, technical and performance criteria. Performance of settling and aeration were evaluated up to 24 h duration. The coarse media filter was intermittently operated with 10 L of greywater in downflow mode while alum was used for chemical coagulation. Experimental results showed that settling up to 6 h did not show significant removal of different pollutants whereas 24 h settling resulted in moderate removal of turbidity and organic content but was not efficient in the removal of nutrients and faecal coliforms. Chemical coagulation reduced 93, 66, 48 and 97% of turbidity, COD, NH4-N and faecal coliforms, respectively from greywater but resulted in excessive sludge generation and is difficult to adopt on-site and requires skilled supervision. Coarse filtration of greywater resulted in 61, 41, 36 and 35% removal of turbidity, COD, PO4-P and faecal coliforms, respectively. Considering different criteria AHP gave coarse filtration as the best pre-treatment option to the granular media filters treating greywater.

Enhanced removal of ciprofloxacin and associated antibiotic-resistant genes from wastewater using a biological aeration filters in combination with Fe3O4-modified zeolite.

Antibiotics release into the water environment through sewage discharge is a significant environmental concern. In the present study, we investigated the removal of ciprofloxacin (CIP) in simulated sewage by biological aeration filter (BAF) equipped with Fe3O4-modified zeolite (Fe3O4@ZF). Fe3O4@ZF were prepared with impregnation method, and the Fe3O4 particles were successfully deposited on the surface of ZF in an amorphous form according to the results of XPS and XRD analysis. The modification also increased the specific surface area (from 16.22 m²/g to 22 m²/g) and pore volume (from 0.0047 cm³/g to 0.0063 cm³/g), improving the adsorption efficiency of antibiotics. Fe3O4 modified ZF improved the treatment performance significantly, and the removal efficiency of CIP in BAF-Fe3O4@ZF was 79%±2.4%. At 10ml/L CIP, the BAF-Fe3O4@ZF reduced the relative abundances of antibiotics resistance genes (ARGs) int, mexA, qnrB and qnrS in the effluent by 57.16%, 39.59%, 60.22%, and 20.25%, respectively, which effectively mitigate the dissemination risk of ARGs. The modification of ZF increased CIP-degrading bacteria abundance, such as Rhizobium and Deinococcus-Thermus, and doubled bacterial ATP activity, promoting CIP degradation. This study offers a viable, efficient method to enhance antibiotic treatment and prevent leakage via sewage discharge.

In-situ treatment of gaseous benzene in fixed-bed biofilter with polyurethane foam: Functional population response and benzene transformation pathway.

Volatile organic compounds emitted from landfills posed adverse effect on health. In this study, gaseous benzene was biologically treated using an in-situ biofilter without air pump. Its performance was investigated and the removal efficiency of benzene reached over 90 %. The decrease in the average benzene concentration was consistent with first-order reaction kinetics. Mycolicibacterium dominated the bacterial consortium (41-57 %) throughout the degradation. Annotation of genes by metagenomic analysis helped to deduce the degradation pathways (benzene degradation, catechol ortho-cleavage and meta-cleavage) and to reveal the contribution of different species to the degradation process. In total, 21 kinds of key genes and 13 enzymes were involved in the three modules of benzene transformation. Mycolicibacter icosiumassiliensis and Sphingobium sp. SCG-1 carried multiple functional genes critically involved in benzene biodegradation. These findings provide technical and theoretical support for the in-situ bioremediation of benzene-contaminated soil and waste gas reduction in landfills.

Exploring biochar and Moringa oleifera seed proteins for greywater remediation on small farms.

This study investigated the potential of using biochar and Moringa oleifera seed proteins for sustainable greywater treatment in rural Kenya. Greywater samples from washing clothes were collected from households in the Kenyan counties of Kwale and Siaya. Two treatment methods, batch stirring and filtration, were used to assess the effectiveness of using biochar and Moringa oleifera seed protein extract together to treat greywater at a household level. Both methods achieved a significant reduction in contaminants: colour was reduced by up to 43% in Kwale and 67% in Siaya, turbidity decreased by 91-98%, and surfactant levels were lowered by 89-93%. There were increases in total organic carbon and total dissolved solids post-treatment, but both methods effectively reduced levels of phosphates, nitrates and iron. This research highlights the potential of using locally available materials for greywater treatment and provides insights into sustainable water management nature-based solutions in the Global South.

Flow rate and kinetics of trace organic contaminants removal in black carbon-amended engineered media filters for improved stormwater runoff treatment.

Urban stormwater runoff is considered a key component of future water supply portfolios for water-stressed cities. Beneficial use of runoff, such as capture for recharge of drinking water aquifers, relies on improved stormwater treatment. Many dissolved constituents, including metals and trace organic contaminants (TrOCs) such as hydrophilic pesticides and poly- and perfluoroalkyl substances (PFASs), are of concern due to their toxicity, persistence, prevalence in stormwater runoff, and poor removal in conventional stormwater control measures. This study explores the operational flow rate limitations of black carbon (BC)-amended engineered media filters for removal of a wide suite of dissolved metals and TrOCs and provides validation for a previously developed predictive TrOC transport model. Column experiments were conducted with face velocities of 40 and 60 cm h-1 to assess Douglas Fir-based biochar and regenerated activated carbon (RAC) filter performance in light of media-contaminant removal kinetic limitations. This study found that increasing the face velocity in BC-amended filters to 40 and 60 cm h-1, which are representative of field conditions, decreased the removal of total suspended solids, turbidity, dissolved hydrophilic TrOCs, and PFASs when expressed as volume treated relative to previous studies conducted at 20 cm h-1. Dissolved metals and hydrophobic TrOCs removal were not substantially affected by the increased flow rates. A predictive 1-d intraparticle pore diffusion-limited sorption model with sorption and effective tortuosity parameters determined previously from experiments conducted at 20 cm h-1 was validated for these higher flow rates. This work provides insights to the kinetic limitations of contaminant removal within biochar and RAC filters and implications for stormwater filter design and operation.

Study on cultivation of aerobic granular sludge and its application in degrading lignin models in the sequencing batch biofilter granular reactor.

In this study, three sequencing batch biofilter granular reactors (SBBGRs) were employed to treat model lignin wastewater containing different lignin models (2,6-dimethoxyphenol, 4-methoxyphenol, and vanillin). After 40 days of cultivation, uniform-shaped aerobic granular sludge (AGS) was successfully developed through nutrient supplementation with synthetic wastewater. During the acclimation stage, the chemical oxygen demand (COD) reduction efficiencies of the three reactors showed a trend of initial decreasing (5-20%) and then recovering to a high reduction efficiency (exceeding 90%) in a short period of time. During the stable operation stage, all three reactors achieved COD reduction efficiencies exceeding 90%. These findings indicated the cultivated AGS's robust resistance to changes in lignin models in water. UV-Vis spectra analysis confirmed the effective degradation of the three lignin models. Microbiological analysis showed that Proteobacteria and Bacteroidetes were always the dominant phyla. At the genus level, while Acinetobacter (15.46%) dominated in the inoculation sludge, Kapabacteriales (7.93%), SBR1031 (11.77%), and Chlorobium (25.37%) were dominant in the three reactors (for 2,6-dimethoxyphenol, 4-methoxyphenol, and vanillin) after degradation, respectively. These findings demonstrate that AGS cultured with SBBGR effectively degrades lignin models, with different dominant strains observed for various lignin models.

Synthesis and characterization of faujasite zeolite membrane for selective enrichment of Arthrobacter sp. in synthetic wastewater.

This paper centers on the preparation and characterization of both a clay support and a faujasite zeolite membrane. Additionally, the study explores the development of bacterial media to assess the performance of these prepared membranes. The faujasite zeolite membrane was created using the hydrothermal method, involving the deposition of a faujasite layer to fine-tune the pore sizes of the clay support. The clay supports were crafted from clay which was sieved to particle size Φ ≤ 63 µm, and compacted with 3.0 wt.% activated carbon, then sintered at 1,000 °C. Distilled water fluxes revealed a decrease from 1,500 L m-2 h-1 to a minimum of 412 L m-2 h-1 after 180 min of filtration. Both membranes were characterized by XRF, XRD, FTIR, adsorption-desorption of nitrogen (N2), and SEM-EDS. PCR technique was used for the identification of the isolated Arthrobacter sp., and the retention of the bacteria on the clay support and the faujasite zeolite membrane were found to be 96 and 99%, respectively. The results showed that the faujasite zeolite membrane passed the clay support due to a narrow pore size of the faujasite zeolite membrane of 2.28 nm compared to 3.55 nm for the clay supports.

Implications of the operation of continuous granular activated carbon filters on the effluent quality.

Granular activated carbon (GAC) filtration is a commonly used method for advanced wastewater treatment. Filters can be operated continuously or discontinuously, with continuous operation not requiring feed flow interruption for backwashing and circulation (B/C). This study investigated the influence of B/C on the effluent quality of continuous filters. Two continuous GAC filters were operated for 1.5 years, with analysis of dissolved substances and particulate matter in the influent and effluent. The results indicated that various B/C modes had no impact on the removal of dissolved organic carbon and organic micropollutants (OMP), achieving an OMP removal of over 70% after 5,600 treated bed volumes (m3 treated wastewater per m3 GAC). However, it was evident that continuous B/C over 2-4 h resulted in increased turbidity, total suspended solids over 30 mg/L and total phosphorus concentrations of 1.3 mg/L in the filter effluent. Additionally, the study demonstrated that longer and more intensive B/C processes resulted in GAC size degradation with AC concentrations of up to 6.9 mg/L in the filter effluent, along with a change in GAC particle size. Furthermore, the importance of pre-filtration in reducing particulate matter in the filter influent and decreasing hydraulic head loss could be demonstrated.

Reduction of Helicobacter pylori cells in rural water supply using slow sand filtration.

Helicobacter pylori is a microorganism that infects 60% of the population and is considered the main cause of atrophic gastritis, gastric and duodenal ulcers, and gastric cancer. Different emerging pathogens have been found in drinking water and their presence is considered to be an important public health problem. For this reason, it is necessary to carry out the validation of reliable technologies for this type of pathogens and evaluate their performance. This paper reports, for the first time, H. pylori reduction in a drinking water pilot plant of two slow sand filters (SSF). Inlet water was taken from a gravel filtration system of a rural water supply in Colombia and then inoculated with viable cells of H. pylori. By determining the Genomic Units (GU) through quantitative Polymerase Chain Reaction (qPCR), the concentration of GU/sample was measured. In the inlet water amplification for SSF1 and SSF2 were 5.13 × 102 ± 4.48 × 102 and 6.59 × 102 ± 7.32 × 102, respectively, while for the treated water they were 7.0 ± 5.6 and 2.05 × 101 ± 2.9 × 101 GU/sample for SSF1 and SSF2, respectively. The SSF pilot plant reached up to 3 log reduction units of H. pylori; therefore, since there is not an H. pylori contamination indicator and its periodic monitoring is financially complicated, the SSF could guarantee the drinking water quality necessity that exists in rural areas and small municipalities in developing countries, where infection rates and prevalence of this pathogen are high.

Impact of p-cresol on hydrogen sulfide and ammonia treatment by biotrickling filter and the production of nitrous oxide.

Biotrickling filter (BTF) is often used for purification of waste gas from swine houses, with vital information still needed regarding interaction effects among multiple gas pollutants removal and also the formation of byproducts especially nitrous oxide (N2O, a strong greenhouse gas) due to the relative high NH3 concentration level compared to other gases. In this study, gas removal and N2O production were compared between two BTFs, where the inlet gas of BTF-1 contained NH3 and H2S while p-cresol was additionally supplied to BTF-2. At inlet load (IL) between 3.67 and 18.91 g m-3 h-1, removal efficiencies of NH3 exceeded 95% for both BTFs. As alternative strategy, adding thiosulfate improved H2S removal. Interestingly, presence of p-cresol to some extent promoted H2S removal at IL of 0.56 g m-3 h-1possibly due to effect on pH value of circulating solution. Similar to NH3, removal efficiencies of p-cresol were higher than 95% at an average IL of 2.98 g m-3 h-1. Gas residence time, pH of circulating solution and inlet loading were identified as key factors affecting BTF performance, but the response of individual gas compound to these factors was not consistent. Overall, p-cresol enhanced N2O generation although the effects were not always significant. High-throughput sequencing results showed that Proteobacteria accounted for the largest proportion of relative abundance and BTF-2 had much richer microbial diversity compared to BTF-1. Thermomonas, Comamonas, Rhodanobacter and other bacterial genus capable of denitrification were detected in both BTFs, and their corresponding abundances in BTF-2 (10.9%, 8.7% and 5.2%) were all greater than those in BTF-1 (0.4%, 0.3% and 2.0%), indicating that more denitrification may occur within BTF-2 and higher N2O could have been generated. This study provided evidence that organic gas components, served as carbon source, may increase the N2O production from BTF when treating waste gases containing NH3.

Removal of endocrine disruptors and pharmaceuticals by graphene oxide-based membranes in water: A review.

Membrane-based water treatment has emerged as a promising solution to address global water challenges. Graphene oxide (GO) has been successfully employed in membrane filtration processes owing to its reversible properties, large-scale production potential, layer-to-layer stacking, great oxygen-based functional groups, and unique physicochemical characteristics, including the creation of nano-channels. This review evaluates the separation performance of various GO-based membranes, manufactured by coating or interfacial polymerization with different support layers such as polymer, metal, and ceramic, for endocrine-disrupting compounds (EDCs) and pharmaceutically active compounds (PhACs). In most studies, the addition of GO significantly improved the removal efficiency, flux, porosity, hydrophilicity, stability, mechanical strength, and antifouling performance compared to pristine membranes. The key mechanisms involved in contaminant removal included size exclusion, electrostatic exclusion, and adsorption. These mechanisms could be ascribed to the physicochemical properties of compounds, such as molecular size and shape, hydrophilicity, and charge state. Therefore, understanding the removal mechanisms based on compound characteristics and appropriately adjusting the operational conditions are crucial keys to membrane separation. Future research directions should explore the characteristics of the combination of GO derivatives with various support layers, by tailoring diverse operating conditions and compounds for effective removal of EDCs and PhACs. This is expected to accelerate the development of surface modification strategies for enhanced contaminant removal.

Pilot-scale performance of gravity-driven ultra-high flux fabric membrane systems for removing small-sized microplastics in wastewater treatment plant effluents.

The ubiquitous nature and environmental impacts of microplastic particles and fibers demand effective solutions to remove such micropollutants from sizable point sources, including wastewater treatment plants and road runoff facilities. While advanced methods, e.g., microfiltration and ultrafiltration, have shown high removal efficiencies of small-sized microplastics (<150 µm), the low flux encountered in these systems implies high operation costs and makes them less effective in high-capacity wastewater facilities. The issue presents new opportunities for developing cheap high-flux membrane systems, deployable in low-to high-income economies, to remove small-sized microplastic and nanoplastics in wastewater. Here, we report on developing an ultra-high flux gravity-driven fabric membrane system, assessed through a laboratory-scale filtration and large-scale performance in an actual wastewater treatment plant (WWTP). The method followed a carefully designed water sampling, pre-treatment protocol, and analytical measurements involving Fourier transform infrared (FTIR) spectroscopy and laser direct infrared (LDIR) imaging. The result shows that the ultra-high flux (permeance = 550,000 L/m2h⋅bar) fabric membrane system can effectively remove small-sized microplastics (10-300 µm) in the secondary effluent of an actual WWTP at high efficiency greater than 96 %. The pilot system demonstrated a continuous treatment capacity of 300,000 L/day through a 1 m2 surface area disc, with steady removal rates of microplastics. These findings demonstrate the practical, cheap, and sustainable removal of small-sized microplastics in wastewater treatment plants, and their potential value for other large-scale point sources, e.g., stormwater treatment facilities.

Role of Microfiltration Membrane Morphology on Nanoparticle Purification to Enhance Downstream Purification of Viral Vectors.

In the rapidly advancing realms of gene therapy and biotechnology, the efficient purification of viral vectors is pivotal for ensuring the safety and efficacy of gene therapies. This study focuses on optimizing membrane selection for viral vector purification by evaluating key properties, including porosity, thickness, pore structure, and hydrophilicity. Notably, we employed adeno-associated virus (AAV)-sized nanoparticles (20 nm), 200 nm particles, and bovine serum albumin (BSA) to model viral vector harvesting. Experimental data from constant pressure normal flow filtration (NFF) at 1 and 2 bar using four commercial flat sheet membranes revealed distinct fouling behaviors. Symmetric membranes predominantly showed internal and external pore blockage, while asymmetric membranes formed a cake layer on the surface. Hydrophilicity exhibited a positive correlation with recovery, demonstrating an enhanced recovery with increased hydrophilicity. Membranes with higher porosity and interpore connectivity showcased superior throughput, reduced operating time, and increased recovery. Asymmetric polyether sulfone (PES) membranes emerged as the optimal choice, achieving ∼100% recovery of AAV-sized particles, an ∼44% reduction in model cell debris (200 nm particles), an ∼35% decrease in BSA, and the fastest operating time of all membranes tested. This systematic investigation into fouling behaviors and membrane properties not only informs optimal conditions for viral vector recovery but also lays the groundwork for advancing membrane-based strategies in bioprocessing.

Comparing Mn-based oxides filters started by KMnO4 versus K2FeO4 for ammonium and manganese removal: Formation mechanism of active species.

A pilot-scale filtration system was adopted to prepare filter media with catalytic activity to remove manganese (Mn2+) and ammonium (NH4+-N). Three different combinations of oxidants (KMnO4 and K2FeO4) and reductants (MnSO4 and FeCl2) were used during the start-up period. Filter R3 started up by KMnO4 and FeCl2 (Mn7+→MnOx) exhibited excellent catalytic property, and the NH4+-N and Mn2+ removal efficiency reached over 80% on the 10th and 35th days, respectively. Filter R1 started up by K2FeO4 and MnSO4 (MnOx←Mn2+) exhibited the worst catalytic property. Filter R2 started up by KMnO4 and MnSO4 (Mn7+→MnOx←Mn2+) were in between. According to Zeta potential results, the Mn-based oxides (MnOx) formed by Mn7+→MnOx performed the highest pHIEP and pHPZC. The higher the pHIEP and pHPZC, the more unfavorable the cation adsorption. However, it was inconsistent with its excellent Mn2+ and NH4+-N removal abilities, implying that catalytic oxidation played a key role. Combined with XRD and XPS analysis, the results showed that the MnOx produced by the reduction of KMnO4 showed early formation of buserite crystals, high degree of amorphous, high content of Mn3+ and lattice oxygen with the higher activity to form defects. The above results showed that MnOx produced by the reduction of KMnO4 was more conducive to the formation of active species for catalytic oxidation of NH4+-N and Mn2+ removal. This study provides new insights on the formation mechanisms of the active MnOx that could catalytic oxidation of NH4+-N and Mn2+.

Bacterial assembly and succession patterns in conventional and advanced drinking water systems: From source to tap.

Bacteria are pivotal to drinking water treatment and public health. However, the mechanisms of bacterial assembly and their impact on species coexistence remain largely unexplored. This study explored the assembly and succession of bacterial communities in two full-scale drinking water systems over one year. We observed a decline in bacterial biomass, diversity, and co-occurrence network complexity along the treatment processes, except for the biological activated carbon filtration stage. The conventional plant showed higher bacterial diversity than the advanced plant, despite similar bacterial concentrations and better removal efficiency. The biological activated carbon filter exhibited high phylogenetic diversity, indicating enhanced bacterial metabolic functionality for organic matter removal. Chlorination inactivated most bacteria but favored some chlorination-resistant and potentially pathogenic species, such as Burkholderia, Bosea, Brevundimonas, and Acinetobacter. Moreover, the spatiotemporal dynamics of the bacterial continuum were primarily driven by stochastic processes, explaining more than 78% of the relative importance. The advanced plant's bacterial community was less influenced by dispersal limitation and more by homogeneous selection. The stochastic process regulated bacterial diversity and influenced the complexity of the species co-occurrence network. These findings deepen our understanding of microbial ecological mechanisms and species interactions, offering insights for enhancing hygienic safety in drinking water systems.

Development of highly bacterial filtration efficient and antibacterial cellulose acetate/gum rosin composite nanofibers for facemask application.

Cellulose acetate (CA) is a non-toxic, renewable, and biodegradable polymeric material that can be effectively electrospuned into bacterial filtration efficient nanofiber membrane for face mask application. However, its fragile and non-antibacterial nature influenced its scalability. In this context, natural antibacterial gum rosin (GR) additive can be explored. Therefore, the present study aimed to produce a CA/GR composite nanofibers membrane for the finest bacterial filtration, excellent antibacterial moiety, and improved tensile properties for facemask application. Hence, in this work, we have studied the effect of GR concentrations (0-15 g) on the needleless electrospinning behavior and fibers' morphology through rheology, electrical conductivity, and SEM analysis. These analyses revealed that GR significantly affects the fibers' spinning behavior, morphology, and diameter of the produced fibers. Later, ATR-FTIR spectroscopy mapped the functional changes in the produced nanofibers that affirmed the integration of GR with CA polymer. This modification resulted in a 3-fold rise in tensile strength and an 11-fold decline in elongation% in 15 g CA/GR composite nanofibers membrane than the control sample. Furthermore, it has shown 98.79 ± 0.10% bacterial filtration efficiency and âˆ¼ 93 % reduction in Staphylococcus Aureus and Klebsiella Pneumoniae bacterial growth, elucidating a high-efficiency level for potential facemask application.