Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 64
Filtrar
1.
Virus Genes ; 60(3): 309-313, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38491264

RESUMO

Adenoviruses (AdVs) have been detected in a wide variety of animals. To date, eight types of AdVs in sheep and two types in goats have been identified, which belong to two distinct genera, Mastadenovirus and Atadenovirus. Typically, the term pneumo-enteritis is used to describe adenovirus-induced disease in small ruminants, which has been associated with both enteric and respiratory symptoms of varying severity. The aim of this study was to detect and identify AdVs of small ruminants belonging to the genera Mastadenovirus and Atadenovirus. For this purpose, diagnostic samples (47 lung, 27 intestine, and two pooled tissue samples including intestine and lung) from 49 small ruminants (39 sheep and 10 goats) were used. Following the viral DNA extraction, PCR was carried out by using the primers targeting the hexon gene in order to detect both mast- and atadenoviruses. Sequencing the amplified fragments revealed the presence of three types of ovine adenovirus (OAdV): OAdV-3, OAdV-4, and OAdV-8. Specifically, OAdV-3 was detected in two sheep and a goat while OAdV-4 and OAdV-8 were found in only one sheep each. There is still limited data on the interaction between the viruses in different adenovirus genera and the detected disease, as well as the genetic diversity of adenoviruses, especially in small ruminants. In conclusion, the detection of AdVs in lung and intestinal tissues of small ruminants in this study suggests that these viruses may have contributed to the disease and/or predisposed to other agents.


Assuntos
Infecções por Adenoviridae , Doenças das Cabras , Cabras , Mastadenovirus , Filogenia , Doenças dos Ovinos , Animais , Cabras/virologia , Ovinos/virologia , Doenças dos Ovinos/virologia , Doenças das Cabras/virologia , Infecções por Adenoviridae/veterinária , Infecções por Adenoviridae/virologia , Mastadenovirus/genética , Mastadenovirus/isolamento & purificação , Mastadenovirus/classificação , Turquia , DNA Viral/genética , Análise de Sequência de DNA , Atadenovirus/genética , Atadenovirus/isolamento & purificação , Atadenovirus/classificação , Pulmão/virologia , Adenoviridae/genética , Adenoviridae/isolamento & purificação , Adenoviridae/classificação , Adenoviridae/patogenicidade
2.
Virology ; 593: 109999, 2024 05.
Artigo em Inglês | MEDLINE | ID: mdl-38368638

RESUMO

We report the discovery and characterization of a novel adenovirus, Zoothera dauma adenovirus (ZdAdV), from a wild bird species, Zoothera dauma (Scaly thrush). This new atadenovirus was discovered by metagenomic sequencing without virus cultivation. Analyses of the full genome sequence revealed that this new virus is a distinct member of the genus Atadenovirus and represents a novel species. ZdAdV has a genome of 34,760 bp with 28 predicted genes and 39% GC content. ZdAdV is the first atadenovirus to contain ORF19, a gene previously found only in aviadenoviruses. Phylogenetic analysis of ORF19 suggests that it was acquired by ZdAdV through horizontal gene transfer from an aviadenovirus. By analyzing all orthologous genes of aviadenovirus, mastadenovirus, atadenovirus, and siadenovirus, we also found potential horizontal gene transfer for the E4 gene in Pigeon aviadenovirus B. Our study widens our knowledge concerning the genetic diversity and evolutionary history of atadenoviruses and their potential for cross-species transmission.


Assuntos
Infecções por Adenoviridae , Atadenovirus , Aviadenovirus , Animais , Atadenovirus/genética , Genoma Viral , Filogenia , Transferência Genética Horizontal , Adenoviridae/genética , Aviadenovirus/genética , Aves , Infecções por Adenoviridae/genética
3.
Avian Pathol ; 53(2): 101-105, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38018364

RESUMO

RESEARCH HIGHLIGHTS: A sandwich ELISA was developed to detect EDSV using the mAbs 5G4 and HRP-6G6.The sandwich ELISA maintained high specificity and sensitivity.The sandwich ELISA had equivalent consistency with real-time PCR assay.


Assuntos
Anticorpos Monoclonais , Atadenovirus , Animais , Ensaio de Imunoadsorção Enzimática/veterinária , Sensibilidade e Especificidade
4.
Arch Razi Inst ; 78(2): 757-765, 2023 04.
Artigo em Inglês | MEDLINE | ID: mdl-37396719

RESUMO

Egg drop syndrome (EDS) is prevalent in industrial poultry globally. This disease is caused by Duck atadenovirus A or EDS virus (EDSV), a member of the genus Atadenovirus under the family Adenoviridae. The disease is attributed to significant economic losses in the poultry industry worldwide due to a drop in egg production, reduction in egg quality, and failure to reach maximum egg production. Oil-adjuvant inactivated vaccines, which are widely used in the poultry industry, provide good protection for immunized chickens against EDS. This study aimed to genetically and phylogenetically analyze the full-length genome of an embryonated chicken egg-adapted EDSV strain 127. After extraction of viral DNA from the allantoic fluid, overlapping fragments of the viral genome sequence were generated by polymerase chain reaction (PCR) using 25 pairs of primers. Purified PCR products were subjected to complete genome sequencing by the next-generation sequencing (NGS) approach. The nucleotide homology observed between genomes of the studied strain and that of the original strain 127 (NC_001813) of laying chickens was 99.9%. Its genome was 33,213 bp in length, with a G + C content of 43.01%. A comparison of the genome sequence of the egg-adapted virus with strain 127 revealed only three non-synonymous single-nucleotide polymorphisms (SNPs) between these viral genome sequences. Two mutations of S320G and I62K out of these SNPs were found within the coding regions of fiber and hypothetical proteins which may play a role in the adaptation of EDSV in the embryonated chicken eggs. The full genome sequencing of EDSV using NGS techniques provides insights into the discovery of genetic variants. Moreover, the genome sequence information of the EDSV provides valuable data for vaccine development in near future.


Assuntos
Infecções por Adenoviridae , Atadenovirus , Animais , Patos/genética , Galinhas , Atadenovirus/genética , Reação em Cadeia da Polimerase , Sequenciamento Completo do Genoma , Infecções por Adenoviridae/veterinária
5.
Viruses ; 14(8)2022 08 13.
Artigo em Inglês | MEDLINE | ID: mdl-36016389

RESUMO

While adenoviruses cause infections in a wide range of vertebrates, members of the genus Atadenovirus, Siadenovirus, and Aviadenovirus predominantly infect avian hosts. Several recent studies on avian adenoviruses have encouraged us to re-visit previously proposed adenovirus evolutionary concepts. Complete genomes and partial DNA polymerase sequences of avian adenoviruses were extracted from NCBI and analysed using various software. Genomic analyses and constructed phylogenetic trees identified the atadenovirus origin from an Australian native passerine bird in contrast to the previously established reptilian origin. In addition, we demonstrated that the theories on higher AT content in atadenoviruses are no longer accurate and cannot be considered as a species demarcation criterion for the genus Atadenovirus. Phylogenetic reconstruction further emphasised the need to reconsider siadenovirus origin, and we recommend extended studies on avian adenoviruses in wild birds to provide finer evolutionary resolution.


Assuntos
Infecções por Adenoviridae , Adenoviridae , Atadenovirus , Aviadenovirus , Siadenovirus , Adenoviridae/genética , Infecções por Adenoviridae/veterinária , Animais , Austrália , Aviadenovirus/genética , Filogenia
6.
Virol J ; 19(1): 89, 2022 05 24.
Artigo em Inglês | MEDLINE | ID: mdl-35610654

RESUMO

Bovine adenovirus 7 (BAdV-7) is an unclassified member of the genus Atadenovirus with a worldwide distribution and has been reported to induce clinical disease of varying severity in infected cattle, ranging from asymptomatic infections to severe enteric or respiratory disease. In this study, we used next-generation sequencing to obtain the first complete genome sequence of a European strain of BadV-7, from pooled spleen and liver tissue obtained from a deceased newborn Limousin calf. Histopathological analysis and electron microscopy showing systemic lesions in multiple organs with intranuclear amphophilic inclusions observed in endothelial cells in multiple peripheral tissues. Virus isolation was readily achieved from tissue homogenate using bovine esophagus cells (KOP-R), a strategy which should facilitate future in vitro or in vivo BAdV-7 studies. Phylogenetic analysis of available genome sequences of BAdV-7 showed that the newly identified strain groups most closely with a recent BAdV-7 strain, SD18-74, from the USA, confirming that this newly identified strain is a member of the Atadenovirus genus. The fiber gene was found to be highly conserved within BAdV-7 strains but was highly divergent in comparison to Ovine adenovirus 7 (OAdV-7) (39.56% aa sequence identity). Furthermore, we report a variable region of multiple tandem repeats between the coding regions of E4.1 and RH5 genes. In summary, the presented pathological and molecular characterization of this case suggests that further research into the worldwide molecular epidemiology and disease burden of BAdV-7 is warranted.


Assuntos
Atadenovirus , Doenças dos Bovinos , Animais , Atadenovirus/genética , Bovinos , Células Endoteliais , Fases de Leitura Aberta , Filogenia , Ovinos
7.
Viruses ; 13(11)2021 10 30.
Artigo em Inglês | MEDLINE | ID: mdl-34835000

RESUMO

Using a broad-range nested PCR assay targeting the DNA-dependent DNA polymerase (pol) gene, we detected adenoviruses in 17 (20.48%) out of 83 fecal samples from small Indian mongooses (Urva auropunctata) on the Caribbean island of St. Kitts. All 17 PCR amplicons were sequenced for the partial pol gene (~300 bp, hereafter referred to as Mon sequences). Fourteen of the 17 Mon sequences shared maximum homology (98.3-99.6% and 97-98.9% nucleotide (nt) and deduced amino acid (aa) sequence identities, respectively) with that of bovine adenovirus-6 (species Bovine atadenovirus E). Mongoose-associated adenovirus Mon-39 was most closely related (absolute nt and deduced aa identities) to an atadenovirus from a tropical screech owl. Mon-66 shared maximum nt and deduced aa identities of 69% and 71.4% with those of atadenoviruses from a spur-thighed tortoise and a brown anole lizard, respectively. Phylogenetically, Mon-39 and Mon-66 clustered within clades that were predominated by atadenoviruses from reptiles, indicating a reptilian origin of these viruses. Only a single mongoose-associated adenovirus, Mon-34, was related to the genus Mastadenovirus. However, phylogenetically, Mon-34 formed an isolated branch, distinct from other mastadenoviruses. Since the fecal samples were collected from apparently healthy mongooses, we could not determine whether the mongoose-associated adenoviruses infected the host. On the other hand, the phylogenetic clustering patterns of the mongoose-associated atadenoviruses pointed more towards a dietary origin of these viruses. Although the present study was based on partial pol sequences (~90 aa), sequence identities and phylogenetic analysis suggested that Mon-34, Mon-39, and Mon-66 might represent novel adenoviruses. To our knowledge, this is the first report on the detection and molecular characterization of adenoviruses from the mongoose.


Assuntos
Adenoviridae/classificação , Adenoviridae/genética , Adenoviridae/isolamento & purificação , Herpestidae/virologia , Infecções por Adenoviridae/veterinária , Infecções por Adenoviridae/virologia , Sequência de Aminoácidos , Animais , Atadenovirus/classificação , Atadenovirus/genética , Atadenovirus/isolamento & purificação , DNA Polimerase Dirigida por DNA , Fezes/virologia , Lagartos/virologia , Mastadenovirus/classificação , Mastadenovirus/genética , Mastadenovirus/isolamento & purificação , Filogenia , Reação em Cadeia da Polimerase , Tartarugas/virologia , Índias Ocidentais
8.
Arch Virol ; 166(10): 2835-2839, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34319454

RESUMO

The bovine adenovirus 7 (BAdV-7) isolate SD18-74 was recovered from lung tissue of calves in South Dakota. The 30,043-nucleotide (nt) genome has the typical organization of Atadenovirus genus members. The sequence shares over 99% nt sequence identity with two Japanese BAdV-7 sequences, followed by 74.9% nt sequence identity with the ovine adenovirus 7 strain OAV287, a member of the species Ovine atadenovirus D. SD18-74 was amplified in both bovine and ovine primary nasal turbinate cells, demonstrating greater fitness in bovine cells. The genomic and biological characteristics of BAdV-7 SD18-74 support the inclusion of the members of the BAdV-7 group in a new species in the genus Atadenovirus.


Assuntos
Infecções por Adenoviridae/veterinária , Atadenovirus/classificação , Atadenovirus/genética , Bovinos/virologia , Infecções por Adenoviridae/virologia , Animais , Atadenovirus/isolamento & purificação , Atadenovirus/fisiologia , Doenças dos Bovinos/virologia , Linhagem Celular , DNA Viral/genética , Genoma Viral/genética , Ovinos , Estados Unidos , Replicação Viral
9.
J Wildl Dis ; 57(2): 313-320, 2021 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-33822152

RESUMO

Adenovirus hemorrhagic disease affects primarily mule deer (Odocoileus hemionus), white-tailed deer (Odocoileus virginianus), Rocky Mountain elk (Cervus canadensis nelsoni), and moose (Alces alces) in their first year of life. The method by which the causative virus, Deer atadenovirus A, is maintained in the environment and transmitted to neonates is unknown. In this study, we investigated the potential transmission of the virus from dam to offspring in Rocky Mountain mule deer (Odocoileus hemionus hemionus) and elk in western Wyoming, US. We sampled dams before parturition during placement of vaginal implant transmitters and at parturition and sampled neonates during capture in their first days of life. We also tested for the virus in mortalities submitted for pathologic examination and laboratory analysis. We detected viral DNA in samples from all time points tested but did not find a connection between positive dams and offspring mortalities associated with adenovirus hemorrhagic disease. Although we did not find direct evidence of transmission events between dams and offspring, asymptomatic animals shedding of Deer atadenovirus A, are a likely source of infection in neonates.


Assuntos
Infecções por Adenoviridae/veterinária , Atadenovirus/classificação , DNA Viral/isolamento & purificação , Cervos/virologia , Transmissão Vertical de Doenças Infecciosas/veterinária , Infecções por Adenoviridae/epidemiologia , Infecções por Adenoviridae/virologia , Sistemas de Identificação Animal , Animais , Animais Recém-Nascidos/virologia , Atadenovirus/isolamento & purificação , Feminino , Vagina/virologia , Eliminação de Partículas Virais , Wyoming
10.
Viruses ; 12(9)2020 09 17.
Artigo em Inglês | MEDLINE | ID: mdl-32957674

RESUMO

Wild birds harbour a large number of adenoviruses that remain uncharacterised with respect to their genomic organisation, diversity, and evolution within complex ecosystems. Here, we present the first complete genome sequence of an atadenovirus from a passerine bird that is tentatively named Passerine adenovirus 1 (PaAdV-1). The PaAdV-1 genome is 39,664 bp in length, which was the longest atadenovirus to be sequenced, to the best of our knowledge, and contained 42 putative genes. Its genome organisation was characteristic of the members of genus Atadenovirus; however, the novel PaAdV-1 genome was highly divergent and showed the highest sequence similarity with psittacine adenovirus-3 (55.58%). Importantly, PaAdV-1 complete genome was deemed to contain 17 predicted novel genes that were not present in any other adenoviruses sequenced to date, with several of these predicted novel genes encoding proteins that harbour transmembrane helices. Subsequent analysis of the novel PaAdV-1 genome positioned phylogenetically to a distinct sub-clade with all others sequenced atadenoviruses and did not show any obvious close evolutionary relationship. This study concluded that the PaAdV-1 complete genome described here is not closely related to any other adenovirus isolated from avian or other natural host species and that it should be considered a separate species.


Assuntos
Adenoviridae/genética , Animais Selvagens/virologia , Genoma Viral , Passeriformes/virologia , Adenoviridae/classificação , Infecções por Adenoviridae/veterinária , Infecções por Adenoviridae/virologia , Animais , Atadenovirus/classificação , Atadenovirus/genética , Sequência de Bases , Genes Virais/genética , Especificidade de Hospedeiro , Filogenia , Proteínas Virais/genética
11.
J Zoo Wildl Med ; 51(1): 232-235, 2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32212569

RESUMO

This study presents the gross and histopathological findings of adenoviral hemorrhagic disease (AHD) in two yearling and one adult mule deer (Odocoileus hemionus). These cases represent the first known outbreak of deer adenovirus (Odocoileus adenovirus 1) in Arizona. Over the span of a month, three female captive mule deer were submitted to Midwestern University's Animal Health Institute for postmortem examination. All of these deer were from the same deer farm and historical findings were similar, consisting of acute presentation of hemorrhagic diarrhea and sudden death. Grossly and histopathologically, all cases had severe pulmonary edema and hemorrhagic enteritis. Additionally, two of the three cases had low numbers of large amphophilic intranuclear inclusions expanding endothelial cells within the small intestine and lungs. Viral PCR of pooled small intestine, lung, and spleen from each of the three cases were positive for deer adenovirus and negative for blue tongue and epizootic hemorrhagic disease.


Assuntos
Infecções por Adenoviridae/veterinária , Atadenovirus/isolamento & purificação , Cervos , Diarreia/veterinária , Hemorragia Gastrointestinal/veterinária , Infecções por Adenoviridae/diagnóstico , Infecções por Adenoviridae/patologia , Animais , Animais de Zoológico , Arizona , Diarreia/diagnóstico , Diarreia/patologia , Feminino , Hemorragia Gastrointestinal/diagnóstico , Hemorragia Gastrointestinal/patologia
13.
Int J Biol Macromol ; 139: 587-595, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31381914

RESUMO

Egg drop syndrome virus (EDSV) is an avian adenovirus that causes markedly decrease in egg production, and in the quality of the eggs when it infects chickens. Until now, EDSV virus-cell interactions are poorly understood, and the cellular receptor is still unknown. In the present study, we determined the atomic structure of the fiber head of EDSV (residues 377-644) at 2.74 Šresolution. Structure comparison with the (chick embryo lethal orphan) CELO long fiber head and human adenovirus fiber heads reveals that the avian adenovirus may interact with the same attachment factor in a unique fashion. Based on the previous studies of CELO virus, we assumed that the chicken coxsackievirus and adenovirus receptor (CAR) may be the attachment factor. We then demonstrate that the chicken CAR serves as a cellular attachment factor for EDSV based on three lines of evidences. Taken together, the results presented here are helpful for further exploring the pathogenesis related to the interaction between EDSV and host cells, and may be used for vaccine development and intervention strategies against EDSV infection.


Assuntos
Atadenovirus/fisiologia , Proteínas do Capsídeo/química , Proteínas do Capsídeo/metabolismo , Modelos Moleculares , Conformação Proteica , Receptores Virais/química , Receptores Virais/metabolismo , Infecções por Adenoviridae/veterinária , Sequência de Aminoácidos , Animais , Células Cultivadas , Patos , Expressão Gênica , Técnicas de Silenciamento de Genes , Humanos , Imagem Óptica , Ligação Proteica , Domínios e Motivos de Interação entre Proteínas , Proteínas Recombinantes , Relação Estrutura-Atividade
14.
Vet Microbiol ; 234: 72-76, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31213274

RESUMO

Adenoviruses are a relatively common infection of reptiles globally and are most often reported in captive central bearded dragons (Pogona vitticeps). We report the first evidence of adenoviruses in bearded dragons in their native habitat in Australia. Oral-cloacal swabs and blood samples were collected from 48 free-ranging bearded dragons from four study populations: western bearded dragons (P. minor minor) from Western Australia (n = 4), central bearded dragons (P. vitticeps) from central Australia (n = 2) and western New South Wales (NSW) (n = 29), and coastal bearded dragons (P. barbata) from south-east Queensland (n = 13). Samples were tested for the presence of adenoviruses using a broadly reactive (pan-adenovirus) PCR and a PCR specific for agamid adenovirus-1. Agamid adenovirus-1 was detected in swabs from eight of the dragons from western NSW and one of the coastal bearded dragons. Lizard atadenovirus A was detected in one of the dragons from western NSW. Adenoviruses were not detected in any blood sample. All bearded dragons, except one, were apparently healthy and so finding these adenoviruses in these animals is consistent with bearded dragons being natural hosts for these viruses.


Assuntos
Adenoviridae/isolamento & purificação , Lagartos/virologia , Adenoviridae/genética , Animais , Atadenovirus/isolamento & purificação , DNA Viral/análise , New South Wales , Reação em Cadeia da Polimerase , Queensland
15.
J Zoo Wildl Med ; 50(1): 238-242, 2019 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-31120684

RESUMO

Adenoviruses are medium-sized DNA viruses with very high host fidelity. The phylogenetic relationships of the adenoviruses strongly resemble that of their hosts, consistent with evolutionary codivergence. The genus Atadenovirus appears to have evolved in squamate hosts. Perhaps the best known of the squamate adenoviruses is Agamid adenovirus 1 (AgAdV1), found most commonly in central bearded dragons (Pogona vitticeps), where it is a prevalent cause of hepatitis/enteritis, especially in young animals. All previous reports of adenoviruses in bearded dragons were AgAdV1. Helodermatid adenovirus 2 (HeAdV2) was first seen in Mexican beaded lizards (Heloderma horridus). Subsequently, partial adenoviral polymerase gene sequence from a western bearded dragon (Pogona minor) in Australia was found to share 99% nucleotide homology with HeAdV2. This article reports the discovery of a virus identical to HeAdV2 in a captive central bearded dragon in Florida and wild Gila monsters (Heloderma suspectum) in Arizona. Additionally, a partial adenoviral polymerase gene sharing 98% homology with this HeAdV2 was discovered in a death adder (Acanthophis antarcticus) in Australia. These findings call into question the provenance of HeAdV2. Further studies of atadenoviral host range, diversity of adenoviruses in captive animals, and characterization of adenoviruses from wild squamates are indicated.


Assuntos
Infecções por Adenoviridae/veterinária , Atadenovirus/isolamento & purificação , Elapidae , Lagartos , Infecções por Adenoviridae/virologia , Animais , Animais de Zoológico , Arizona , Florida , Austrália Ocidental
16.
Poult Sci ; 98(1): 404-412, 2019 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-30690613

RESUMO

Egg drop syndrome virus (EDSV), a member of the family Adenoviridae and an economically important pathogen with a broad host range, leads to markedly decreased egg production. However, the molecular mechanism underlying the host-EDSV interaction remains unclear. Here, we performed high-throughput RNA sequencing (RNA-Seq) to study the dynamic changes in host gene expression at 6, 12, and 24 hours post-infection in duck embryo fibroblasts (DEFs) infected with EDSV. Atotal of 441 differentially expressed genes (DEGs) were identified after EDSV infection. Gene Ontology category and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that these DEGs were associated with multiple biological functions, including signal transduction, host immunity, virus infection, cell apoptosis, cell proliferation, and pathogenicity-related and metabolic process signaling pathways. We screened and identified 12 DEGs for further examination by using qRT-PCR. The qRT-PCR and RNA-Seq results were highly consistent. This study analyzed viral infection and host immunity induced by EDSV infection from a novel perspective, and the results provide valuable information regarding the mechanisms underlying host-EDSV interactions, which will prove useful for the future development of antiviral drugs or vaccines for poultry, thus benefiting the entire poultry industry.


Assuntos
Infecções por Adenoviridae/veterinária , Doenças das Aves Domésticas/virologia , Transcriptoma , Infecções por Adenoviridae/imunologia , Infecções por Adenoviridae/metabolismo , Infecções por Adenoviridae/virologia , Animais , Atadenovirus/patogenicidade , Patos , Embrião não Mamífero/virologia , Fibroblastos/virologia , Interações entre Hospedeiro e Microrganismos , Doenças das Aves Domésticas/genética , Doenças das Aves Domésticas/imunologia , Doenças das Aves Domésticas/metabolismo , Análise de Sequência de RNA/veterinária
17.
J Vet Diagn Invest ; 31(1): 78-82, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30541409

RESUMO

Seven colostrum-deprived, 3-4-wk-old Rambouillet-Hampshire lambs were inoculated via the mucous membranes with deer adenovirus (DAdV) and monitored for clinical signs for 21 d post-inoculation at which time animals were euthanized and postmortem examinations were performed. Pre-inoculation and post-inoculation serum samples were tested for antibodies to DAdV, ovine adenovirus 7, bovine adenovirus 7, and goat adenovirus 1. Evidence for DAdV infection was determined by virus isolation, PCR tests, and histopathology with immunohistochemistry tests for DAdV. No clinical signs or lesions consistent with adenoviral hemorrhagic disease (AHD) in deer were seen in the lambs, and the lambs did not seroconvert to DAdV. DAdV was not detected by PCR, virus isolation, or immunohistochemistry in any of the samples tested from the lambs. A positive control deer similarly inoculated with DAdV developed fatal AHD 1 wk post-inoculation. Our colostrum-deprived lambs did not become infected when inoculated with DAdV.


Assuntos
Infecções por Adenoviridae/veterinária , Atadenovirus/isolamento & purificação , Colostro/imunologia , Doenças dos Ovinos/virologia , Infecções por Adenoviridae/imunologia , Animais , Animais Domésticos , Animais Recém-Nascidos , Animais Lactentes , Atadenovirus/imunologia , Feminino , Imuno-Histoquímica/veterinária , Reação em Cadeia da Polimerase/veterinária , Gravidez , Ovinos , Doenças dos Ovinos/imunologia
18.
J Vet Diagn Invest ; 30(4): 530-537, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29582709

RESUMO

We reviewed case records from the California Animal Health and Food Safety (CAHFS) laboratory and the California Department of Fish and Wildlife (CDFW) spanning 25 years (1990-2014) for all deer accessions submitted to CAHFS for pathology and/or histopathology, with and without a diagnosis of adenoviral hemorrhagic disease (AHD), in order to determine the prevalence of AHD in California. We also examined spatial and temporal distribution, age, and mule deer subspecies in deer that died from AHD. Of 483 deer submitted to CAHFS for diagnostic testing in 1990-2014, 17.2% were diagnosed with confirmed AHD, and 26.5% were confirmed plus suspected cases of AHD. Columbian black-tailed deer ( Odocoileus hemionus columbianus), particularly fawns and juveniles, were most frequently affected. Deer adenovirus ( Odocoileus adenovirus 1; OdAdV-1) was detected by immunohistochemistry in archived CDFW formalin-fixed, paraffin-embedded tissues from deer that died in mortality events in 1981, 1983, and 1986-1987. OdAdV-1 is a common cause of hemorrhagic disease mortality events in California deer, and mortality as a result of AHD is documented as early as 1981.


Assuntos
Infecções por Adenoviridae/veterinária , Animais Selvagens , Cervos , Infecções por Adenoviridae/epidemiologia , Animais , Atadenovirus/isolamento & purificação , California/epidemiologia , Feminino , Imuno-Histoquímica/veterinária , Masculino , Estudos Retrospectivos
19.
BMC Vet Res ; 14(1): 49, 2018 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-29439721

RESUMO

BACKGROUND: Egg drop syndrome (EDS), caused by the adenovirus "egg drop syndrome virus" (EDSV) causes severe economic losses through reduced egg production in breeder and layer flocks. The diagnosis of EDSV has been done by molecular tools since its complete genome sequence was identified. In order to enhance the capabilities of the real-time fluorescence loop-mediated isothermal amplification (RealAmp) assay, we aimed to apply the method for direct detection of the EDSV without viral DNA extraction. In order to detect the presence of the EDSV DNA, three pairs of primers were designed, from the conserved region of fiber gene of the EDSV. RESULTS: For our assay, test and control samples were directly used in the reaction mixture in 10-fold serial dilution. The target DNA was amplified at 65 °C, which yield positive results in a relatively short period of 40-45 min. The method reported in this study is highly sensitive as compared to polymerase chain reaction (PCR) and showed no sign of cross-reactivity or false positive results. The RealAmp accomplished specific identification of EDSV among a variety of poultry disease viruses. CONCLUSIONS: The direct RealAmp can be used to detect the presence of EDSV. As our result showed, the RealAmp method could be suitable for the direct detection of other DNA viruses.


Assuntos
Infecções por Adenoviridae/veterinária , Atadenovirus/genética , Técnicas de Amplificação de Ácido Nucleico/veterinária , Doenças das Aves Domésticas/diagnóstico , Infecções por Adenoviridae/diagnóstico , Infecções por Adenoviridae/virologia , Animais , Células Cultivadas , DNA Viral/genética , Técnicas de Amplificação de Ácido Nucleico/métodos , Reação em Cadeia da Polimerase/veterinária , Doenças das Aves Domésticas/virologia , Sensibilidade e Especificidade
20.
Structure ; 25(10): 1562-1573.e5, 2017 10 03.
Artigo em Inglês | MEDLINE | ID: mdl-28943338

RESUMO

Although non-human adenoviruses (AdVs) might offer solutions to problems posed by human AdVs as therapeutic vectors, little is known about their basic biology. In particular, there are no structural studies on the complete virion of any AdV with a non-mammalian host. We combine mass spectrometry, cryo-electron microscopy, and protein crystallography to characterize the composition and structure of a snake AdV (SnAdV-1, Atadenovirus genus). SnAdV-1 particles contain the genus-specific proteins LH3, p32k, and LH2, a previously unrecognized structural component. Remarkably, the cementing protein LH3 has a trimeric ß helix fold typical of bacteriophage host attachment proteins. The organization of minor coat proteins differs from that in human AdVs, correlating with higher thermostability in SnAdV-1. These findings add a new piece to the intriguing puzzle of virus evolution, hint at the use of cell entry pathways different from those in human AdVs, and will help development of new, thermostable SnAdV-1-based vectors.


Assuntos
Atadenovirus/química , Proteínas do Capsídeo/química , Capsídeo/química , Répteis/virologia , Sequência de Aminoácidos , Animais , Atadenovirus/genética , Proteínas do Capsídeo/genética , Microscopia Crioeletrônica , Cristalografia por Raios X , Espectrometria de Massas , Modelos Moleculares , Estrutura Secundária de Proteína
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...