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1.
Int J Biol Macromol ; 264(Pt 1): 130536, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38432273

RESUMO

Tremella fuciformis polysaccharide (TFPS) is a natural mushroom mucopolysaccharide widely used in health foods, medical care, cosmetic and surgical materials. In this study, we developed an efficient strategy for the repeated batch production of highly bioactive TFPS from the agro-industrial residue cane molasses. Cane molasses contained 39.92 % sucrose (w/w), 6.36 % fructose and 3.53 % glucose, all of which could be utilized by T. fuciformis spores, whereas, the TFPS production efficiency only reached 0.74 g/L/d. Corn cobs proved to be the best immobilized carrier that could tightly absorb spores and significantly shorten the fermentation lag period. The average yield of TFPS in eight repeated batch culture was 5.52 g/L with a production efficiency of 2.04 g/L/d. The average fermentation cycle after optimization was reduced by 61.61 % compared with the initial conditions. Compared to glucose as a carbon source, cane molasses significantly increased the proportion of low-molecular-weight TFPS (TFPS-2) in total polysaccharides from 3.54 % to 17.25 % (w/w). Moreover, TFPS-2 exhibited potent antioxidant capacity against four free radicals (O2-, ABTS+, OH, and DPPH). In conclusion, this study lays the foundation for the efficient conversion of cane molasses and production of TFPS with high bioactivity.


Assuntos
Basidiomycota , Técnicas de Cultura Celular por Lotes , Melaço , Bengala , Polissacarídeos/farmacologia , Polissacarídeos/química , Fermentação , Glucose
2.
Artigo em Inglês | MEDLINE | ID: mdl-38466607

RESUMO

This paper introduces a walking path generation method specifically developed for the Smart Cane, which is a RNA (Robotic Navigation Assistance Device) aimed at enhancing indoor navigation for visually impaired individuals. The proposed approach combines the utilization of a LIPM (Linear Inverse Pendulum Model) and LFPC (Linear Foot Placement Controller) motion primitives to generate walking paths specifically designed for visually impaired individuals. The primary objective is to generate paths that conform to human motion constraints, thereby guaranteeing an efficient and natural navigation experience. Integrating autonomous navigation framework, the Smart Cane facilitates safe and effective guidance for visually impaired participants in the indoor environments. Furthermore, comparative experiments have been conducted to validate the effectiveness of the proposed method, providing evidence of its capability to generate walking paths that conform to human motion constraints. The experiment results indicate that the proposed walking path generation method is a promising solution to enhance the navigation experience of visually impaired individuals.


Assuntos
Auxiliares Sensoriais , Pessoas com Deficiência Visual , Humanos , Bengala , Caminhada
3.
Food Funct ; 15(4): 1977-1993, 2024 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-38277180

RESUMO

The present study is designed to study the efficiency of plant-based hydrocolloids for the efficient clarification of sugarcane juice and subsequent production of non-centrifugal sugars (NCSs). NCSs are generally produced with lime or other inorganic solids as a clarification agent, often leading to products with a bland taste and dark color. This work is a first of its kind, where plant-based hydrocolloids such as starch, xanthan gum, and guar gum are used for clarification studies. Clarification efficiency was evaluated in terms of separation efficiency, turbidity removal, sucrose content, color transmittance, and rheology studies. Preliminary studies revealed that starch showed a better separation efficiency of 78% compared to other hydrocolloids, and further rheology studies of starch-clarified juice showed a favourable shear-thickening (dilatant, n = 1.382) behaviour, whereas the other two hydrocolloids showed an unfavourable shear-thinning (pseudo plastic, n < 0.9) behaviour. Eventually, starch was found to be a better clarification agent and is proposed as an alternative to lime-based clarification. Solidification studies were performed with starch at various concentrations (0.02-0.04%), pH (6.8-7.2), and temperature (80 °C-100 °C), and it was found that NCSs produced via starch clarification showed superior properties compared with traditional lime-based clarification processes.


Assuntos
Compostos de Cálcio , Bengala , Coloides , Óxidos , Coloides/química , Polissacarídeos Bacterianos/química , Amido/química , Reologia , Viscosidade
4.
Anat Histol Embryol ; 53(1): e12990, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-37874623

RESUMO

The subcommissural organ (SCO) is a well-developed gland present in the brain of vertebrates. The SCO secretes glycoproteins into the circulating cerebrospinal fluid and these assemble to form Reissner's fibre. It also plays an important function in neurogenesis and axonal guidance during embryogenesis. This study delves into the microscopic anatomy of the SCO in the adult greater cane rat (GCR), shedding light on its histoarchitectural characteristics. By utilizing histological techniques and microscopic analysis, we investigated the SCO's location and cellular composition within the brain of adult GCR. Our findings showed that the SCO in this species is located ventrally to the posterior commissure (PC) and dorsally to the third ventricle. The SCO consists of specialized ependymal or nuclear cell layer and apical processes lining the third ventricle. Moreover, the SCO's proximity to the PC and the third ventricle highlights its strategic position within the brain's ventricular system. With immunohistochemical analyses, the SCO cells expressed glial fibrillary protein when immunolabelled with Glial fibrillary acid protein (GFAP) antibody, a marker for astrocytes/astrocytic-like cells. Few microglia-like cells were immuno-positive for Ionized calcium-binding adapter molecule 1 (Iba1) antibody, that are existing within the SCO. However, the SCO in the GCR showed a negative immunostaining to NeuN antibody. This study contributes to our understanding of the microscopic anatomy of the SCO in a lesser-studied mammalian species. Further research into the SCO's functional significance especially during development in the GCR, may hold promise for more insights into neurological health and pathology.


Assuntos
Roedores , Órgão Subcomissural , Ratos , Animais , Órgão Subcomissural/metabolismo , Órgão Subcomissural/ultraestrutura , Bengala
5.
Viruses ; 15(11)2023 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-38005915

RESUMO

Hantavirus pulmonary syndrome (HPS) is a rodent-borne zoonotic disease that is endemic throughout the Americas. Agricultural activities increase exposure to wild rodents, especially for sugarcane cutters. We carried out a survey of the epidemiological aspects of HPS and investigated the prevalence of hantavirus infection in the sugarcane cutter population from different localities in the Brazilian Midwest region. We conducted a retrospective study of all confirmed HPS cases in the state of Goiás reported to the National HPS surveillance system between 2007 and 2017, along with a seroepidemiological study in a population of sugarcane cutters working in Goiás state in 2016, using the anti-hantavirus (Andes) ELISA IgG. A total of 634 serum samples from cane cutters were tested for hantavirus antibodies, with 44 (6.9%) being IgG-reactive according to ELISA. The destination of garbage was the only statistically significant variable (p = 0.03) related to the detection of hantavirus IgG (p < 0.05). We described the epidemiological profile of reported hantavirus cases in Goiás-a highly endemic area for HPS, and where the seroepidemiological study was conducted. Our results increase our knowledge about hantavirus infections in Brazil and highlight the vulnerability of sugarcane cutters to a highly lethal disease that, to date, has no specific treatment or vaccination.


Assuntos
Doenças Transmissíveis , Infecções por Hantavirus , Síndrome Pulmonar por Hantavirus , Orthohantavírus , Doenças dos Roedores , Animais , Síndrome Pulmonar por Hantavirus/epidemiologia , Brasil/epidemiologia , Estudos Soroepidemiológicos , Estudos Retrospectivos , Bengala , Infecções por Hantavirus/epidemiologia , Imunoglobulina G , Roedores , Anticorpos Antivirais
6.
Molecules ; 28(17)2023 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-37687063

RESUMO

As a biodegradable and renewable material, polylactic acid is considered a major environmentally friendly alternative to petrochemical plastics. Microbial fermentation is the traditional method for lactic acid production, but it is still too expensive to compete with the petrochemical industry. Agro-industrial wastes are generated from the food and agricultural industries and agricultural practices. The utilization of agro-industrial wastes is an important way to reduce costs, save energy and achieve sustainable development. The present study aimed to develop a method for the valorization of Zizania latifolia waste and cane molasses as carbon sources for L-lactic acid fermentation using Rhizopus oryzae LA-UN-1. The results showed that xylose derived from the acid hydrolysis of Z. latifolia waste was beneficial for cell growth, while glucose from the acid hydrolysis of Z. latifolia waste and mixed sugars (glucose and fructose) from the acid hydrolysis of cane molasses were suitable for the accumulation of lactic acid. Thus, a three-stage carbon source utilization strategy was developed, which markedly improved lactic acid production and productivity, respectively reaching 129.47 g/L and 1.51 g/L·h after 86 h of fermentation. This work demonstrates that inexpensive Z. latifolia waste and cane molasses can be suitable carbon sources for lactic acid production, offering an efficient utilization strategy for agro-industrial wastes.


Assuntos
Melaço , Rhizopus oryzae , Bengala , Resíduos Industriais , Ácido Láctico , Carbono , Glucose
7.
BMC Psychol ; 11(1): 253, 2023 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-37649118

RESUMO

BACKGROUND: The white cane is globally an important tool in orientation and mobility for blind and visually impaired people, but it is not often used. The goal of this study was to develop and validate the white cane use perceived advantages and disadvantages (WCPAD) questionnaire for detecting effective factors in the use or non-use of canes. METHOD: The initial questionnaire items were extracted through semi-structured interviews with 21 blind or severely impaired vision individuals under two main themes, the perceived advantages, and disadvantages of using a white cane. Linguistic validity and writing evaluation with seven experts, face validity with 23 blind persons, content validity ratio (CVR), and content validity index (CVI) were used for assessment of validity. Internal and external reliability assessment was done using Cronbach's alpha and intra-class correlation coefficient (ICC). Explanatory factor analysis was conducted to identify the factors of the questionnaire; then, corrected item-total correlations, convergent and discriminant validity, and Confirmatory Factor Analyses (CFA) were done, and model fit indices were reported. A total of 320 blind and severe visual impaired individuals (138 males and 182 females) participated in the study. The final questionnaire contained 12 questions in perceived advantages and 21 in perceived disadvantages. RESULTS: Three factors (social participation, mobility, and culture) extracted for perceived advantages which explained 67.95% of the total variance, Cronbach-α for the three extracted factors was between 0.75 and 0.91. Four factors (social, safety, ergonomics, and family) for perceived disadvantages were extracted which explained 50.98% of the total variance. Cronbach-α for the four extracted factors was from 0.75 to 0.88. CFA confirmed the final models for perceived advantages and disadvantages. CONCLUSION: WCPAD questionnaire has good reliability and validity, and the factors obtained from factor analysis can measure the reasons for using or otherwise of white canes.


Assuntos
Bengala , Ergonomia , Feminino , Masculino , Humanos , Reprodutibilidade dos Testes , Análise Fatorial , Redação
8.
PLoS One ; 18(8): e0290167, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37582116

RESUMO

In practical production, cane stems with buds are generally used as seed for propagation. However, long-terms cane stems only easily lead to some problems such as disease sensitivity, quality loss, etc. Recently, cane seedings, which are produced by tissue culture were used in sugarcane production, but few studies on cane health related to tissue culture seedings. Therefore, to evaluate the immunity and health of sugarcanes growing from different reproduction modes, the endophytic microbial compositions in cane roots between stem and tissue culture seedlings were analyzed using high-throughput techniques. The results showed that the endophytic microbial compositions in cane roots were significant differences between stem and tissue culture seedlings. At the genus level, Pantoea, Bacillus, Streptomyces, Lechevalieria, Pseudomonas, Nocardioides, unclassified_f__Comamonadaceae enriched as the dominant endophytic bacterial genera, and Rhizoctonia, Sarocladium, Scytalidium, Wongia, Fusarium, unclassified_f__Phaeosphaer, unclassified_c__Sordariom, unclassified_f__Stachybot, Poaceascoma, Microdochium, Arnium, Echria, Mycena and Exophiala enriched as the dominant endophytic fungal genera in cane roots growing from the tissue culture seedlings. In contrast, Mycobacterium, Massilia, Ralstonia, unclassified_f__Pseudonocardiacea, norank_f__Micropepsaceae, Leptothrix and Bryobacter were the dominant endophytic bacterial genera, and unclassified_k__Fungi, unclassified_f__Marasmiaceae, Talaromyces, unclassified_c__Sordariomycetes and Trichocladium were the dominant endophytic fungal genera in cane roots growing from stem seedlings. Additionally, the numbers of bacterial and fungal operational taxonomic units (OTUs) in cane roots growing from tissue culture seedlings were significantly higher than those of stem seedlings. It indicates that not only the endophytic microbial compositions in cane roots can be shaped by different propagation methods, but also the stress resistance of sugarcanes can be improved by the tissue culture propagation method.


Assuntos
Actinomycetales , Agaricales , Ascomicetos , Fungos não Classificados , Fusarium , Sordariales , Streptomyces , Bengala , Raízes de Plantas/microbiologia , Endófitos
9.
Pest Manag Sci ; 79(9): 3334-3341, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-37156732

RESUMO

BACKGROUND: Organic viticulture seeks sustainable alternatives for eco-toxic copper fungicides to control downy mildew caused by Plasmopara viticola. (Poly)phenol-rich extracts of agricultural byproducts are known to possess antifungal activity, but high production costs often limit their actual implementation. RESULTS: We developed and produced novel ligninsulfonate-based grape cane extract (GCE) formulations and an apple extract on a pilot plant scale, including a detailed (poly)phenol characterization by high-performance liquid chromatography photodiode array mass spectrometry (HPLC-PDA-MS). Our GCE formulations alone reduced downy mildew disease severity in greenhouse trials by 29%-69% in a dose-dependent manner, whereas a standard application of the copper-based agent alone reached ~56%. When applied together, disease severity was diminished by 78%-92%, revealing a synergistic effect that depended on the mixture ratio. Combining GCE formulations with the apple extract, additive effects were found (80% disease severity reduction). CONCLUSION: The studied plant extracts are proposed to both substitute for and synergistically reinforce copper fungicides in grapevine downy mildew control. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.


Assuntos
Fungicidas Industriais , Malus , Oomicetos , Vitis , Antifúngicos/farmacologia , Fungicidas Industriais/farmacologia , Fungicidas Industriais/análise , Vitis/microbiologia , Cobre/farmacologia , Bengala , Doenças das Plantas/prevenção & controle , Doenças das Plantas/microbiologia , Fenóis/farmacologia , Extratos Vegetais/farmacologia , Extratos Vegetais/química
10.
Can Vet J ; 64(3): 257-261, 2023 03.
Artigo em Inglês | MEDLINE | ID: mdl-36874538

RESUMO

A 12-year-old castrated male Cane Corso dog was presented with cervical swelling, lethargy, anorexia, and cough. An extensive neck mass with necrotic cysts was observed, severely adherent to surrounding tissues. Based on diagnostic imaging including ultrasound, computed tomography, and fine-needle aspiration cytology, paraesophageal abscess was tentatively diagnosed. However, after the mass was surgically removed, a diagnosis of thyroid carcinosarcoma composed of neoplastic cell populations with epithelial and mesenchymal origins was made via histopathology and immunohistochemistry. The dog died of a recurrent mass with pulmonary metastasis 105 d after surgery. This report describes a rare type of canine thyroid cancer, thyroid carcinosarcoma, preoperatively masquerading as an abscess and postoperatively confirmed by histopathology. Key clinical message: Thyroid carcinosarcoma, despite its rarity in dogs, should be included in the differential diagnoses of cervical mass especially with an aggressive progression.


Carcinosarcome thyroïdien déguisé en abcès paraoesophagien chez un chien Cane Corso. Un chien Cane Corso mâle castré de 12 ans a été présenté avec de l'enflure cervicale, de la léthargie, de l'anorexie et une toux. Une masse étendue du cou avec des kystes nécrotiques a été observée, adhérente fortement aux tissus environnants. Sur la base de l'imagerie diagnostique comprenant l'échographie, la tomodensitométrie et la cytologie par aspiration à l'aiguille fine, un abcès paraoesophagien a été provisoirement diagnostiqué. Cependant, après l'ablation chirurgicale de la masse, un diagnostic de carcinosarcome thyroïdien composé de populations de cellules néoplasiques d'origine épithéliale et mésenchymateuse a été posé par histopathologie et immunohistochimie. Le chien est décédé d'une masse récurrente avec métastase pulmonaire 105 jours après la chirurgie. Ce rapport décrit un type rare de cancer de la thyroïde canine, le carcinosarcome thyroïdien, se faisant passer pour un abcès en préopératoire et confirmé en postopératoire par histopathologie.Message clinique clé:Le carcinosarcome thyroïdien, malgré sa rareté chez le chien, doit être inclus dans les diagnostics différentiels de masse cervicale surtout à évolution agressive.(Traduit par Dr Serge Messier).


Assuntos
Carcinossarcoma , Doenças do Cão , Neoplasias da Glândula Tireoide , Masculino , Cães , Animais , Abscesso/veterinária , Bengala , Carcinossarcoma/veterinária , Neoplasias da Glândula Tireoide/veterinária
11.
Molecules ; 28(6)2023 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-36985611

RESUMO

2,3,5,6-Tetramethylpyrazine (TTMP) is an active ingredient of Ligusticum wallichii Franch. It can be used in medicine and food fields. In this study, Bacillus sp. TTMP20 was applied to produce TTMP using cane molasses as a carbon source. After pretreatment with phosphoric acid, 170 mL/L treated molasses, combined with 10 g/L yeast powder, 30 g/L tryptone and 30 g/L (NH4)2HPO4 were used for fermentation. After 36 h, TTMP output reached the highest value of 208.8 mg/L. The yield of TTMP using phosphoric acid-treated molasses as carbon source was 145.59% higher than control. Under the sulfuric acid treatment process of molasses (150 g), the maximum yield of TTMP was 895.13 mg/L, which was 183.18% higher than that of untreated molasses (316.1 mg/L). This study demonstrated that molasses is a high-quality and inexpensive carbon source for the manufacture of TTMP, laying the groundwork for the future industrial production of TTMP.


Assuntos
Bacillus , Melaço , Bengala , Fermentação , Carbono , Saccharomyces cerevisiae
12.
Spine Deform ; 11(4): 1027-1030, 2023 07.
Artigo em Inglês | MEDLINE | ID: mdl-36952137

RESUMO

PURPOSE: To manage severe angular chin-on-chest deformity. METHODS: A single midline incision and periosteal dissection were utilized to expose from C2 to T6. Bilateral C2 pars screws, C3 to C7 lateral mass screws, and T1 to T6 pedicle screws were placed. Following the placement of screws, multiple two column posterior osteotomies and interlaminar decompressions from C6 to T3 were performed to amplify both the sagittal and coronal corrections. Titanium rods were utilized in light of the patient's known osteopenia and nickel allergy. As such, the construct was augmented via the use of a third accessory rod. This third titanium rod was placed into a supplementary translaminar screw with three connectors to the right-sided main rod. Once the lordotic configuration of the contralateral main rod was secured, an additional corrective maneuver of gentle distraction across this third rod was employed to assist with coronal correction. RESULTS: In this patient with osteopenia, a known nickel allergy, and significant cervical imbalance, the Candy Cane construct allowed for a durable correction of the severe sagittal and coronal plane deformity. The chin-brow angle was corrected by 44°. The coronal Cobb angle improved by 10°. On long-term follow-up, the patient reported continued satisfaction with the operation and was able to perform his activities of daily living. CONCLUSION: A one-stage, posterior approach along with construct augmentation, with a third rod hooked into a supplementary C2 translaminar screw, can be employed for the correction of chin-on-chest kyphoscoliosis.


Assuntos
Cifose , Parafusos Pediculares , Escoliose , Humanos , Atividades Cotidianas , Bengala , Queixo , Níquel , Titânio , Cifose/cirurgia , Escoliose/cirurgia
13.
Langmuir ; 39(12): 4394-4405, 2023 03 28.
Artigo em Inglês | MEDLINE | ID: mdl-36913721

RESUMO

The development of a highly selective, simple, and rapid detection method for nitrofuran antibiotics (NFs) is of great significance for food safety, environmental protection, and human health. To meet these needs, in this work, cyan-color highly fluorescent N-doped graphene quantum dots (N-GQDs) were synthesized using cane molasses as the carbon source and ethylenediamine as the nitrogen source. The synthesized N-GQDs have an average particle size of 6 nm, a high fluorescence intensity with 9 times that of undoped GQDs, and a high quantum yield (24.4%) which is more than 6 times that of GQDs (3.9%). A fluorescence sensor based on N-GQDs for the detection of NFs was established. The sensor shows advantages of fast detection, high selectivity, and sensitivity. The limit of detection for furazolidone (FRZ) was 0.29 µM, the limit of quantification (LOQ) was 0.97 µM, and the detection range was 5-130 µM. The fluorescence quenching mechanism of the sensor was explored by fluorescence spectroscopy, UV-vis absorption spectroscopy, Stern-Volmer quenching constant, Zeta potential, UV-vis diffuse reflectance spectroscopy, and cyclic voltammetry. A fluorescence quenching mechanism of dynamic quenching synergized with photoinduced electron transfer was revealed. The developed sensor was also successfully applied for detecting FRZ in various real samples, and the results were satisfactory.


Assuntos
Grafite , Nitrofuranos , Pontos Quânticos , Humanos , Grafite/química , Antibacterianos , Pontos Quânticos/química , Bengala , Elétrons , Melaço , Nitrogênio/química
14.
J Geriatr Oncol ; 14(3): 101451, 2023 04.
Artigo em Inglês | MEDLINE | ID: mdl-36871444

RESUMO

INTRODUCTION: Approximately 25% of older cancer survivors (i.e., ≥ 65 years, with cancer history) use ≥1 mobility device, surpassing usage by other older adults. Few tools exist for older "survivors" to regain function or follow lifestyle recommendations. Our goal was to explore opportunities to leverage technology-enabled mobility devices, such as the "smart cane," to support mobility goals in these survivors. The research objective was to assess perceptions related to acceptability, usability and preferences of participants regarding technology-enabled mobility devices in everyday life. MATERIALS AND METHODS: We used a convergent mixed-methods design, analyzing quantitative data followed by qualitative focus groups. A pre-survey derived from the Senior Technology Acceptance Model assessed the acceptability of technology-enabled devices among participants, who also participated in one of three focus groups delivered via Zoom. The Zoom sessions included facilitated 90-min discussions and video demonstration of the smart cane. Focus group sessions were recorded and transcribed verbatim and thematic content analysis was conducted. RESULTS: We recruited 12 older US survivors. Participants were 58% female, aged 68-86, and 16% non-White. From a pre-survey of participants, 83% said that they liked the idea of technology-enhanced mobility device and 100% said they thought they could be skillful at using a technology-enabled device if training was provided. Though participants were enthusiastic about the smart cane overall and felt the smart cane supported independence for older adults, the themes revealed concerns about safety, accessibility and technology support, as well as the concern for negative impact on self-image due to use of a mobility device. There was a strong preference for working with clinical professionals as the most trusted sources for referrals, if a smart cane was suggested. DISCUSSION: Older survivors in our sample found the smart cane very acceptable, and supportive of independence for older adults with cancer and other conditions. Participants also provided many insights that revealed additional research needed to support access, safety and usability for older adults, older survivors and caregivers, especially by partnering with clinical professionals.


Assuntos
Sobreviventes de Câncer , Neoplasias , Tecnologia Assistiva , Humanos , Feminino , Idoso , Masculino , Bengala , Inquéritos e Questionários , Sobreviventes , Neoplasias/terapia
15.
Bioresour Technol ; 376: 128833, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-36889604

RESUMO

Cane molasses (CM) is a sugar-rich agro-industrial byproduct. The purpose of this study is to synthesize docosahexaenoic acid (DHA) in Schizochytrium sp. by using CM. The single factor analysis showed that sucrose utilization was the main factor limiting the utilization of CM. Therefore, the endogenous sucrose hydrolase (SH) was overexpressed in Schizochytrium sp., which enhanced the sucrose utilization rate 2.57-fold compared to the wild type. Furthermore, adaptive laboratory evolution was used to further improve sucrose utilization from CM. Comparative proteomics and RT-qPCR were used out to analyze the metabolic differences of evolved strain grown on CM and glucose, respectively. Finally, a constant flow rate CM feeding strategy was implemented, whereby the DHA titer and lipid yield of the final strain OSH-end reached 25.26 g/L and 0.229 g/g sugar, respectively. This study demonstrated the CM is a cost-effective carbon source for industrial DHA fermentation.


Assuntos
Ácidos Docosa-Hexaenoicos , Estramenópilas , Melaço , Bengala , Fermentação , Glucose/metabolismo , Estramenópilas/genética , Estramenópilas/metabolismo
17.
Sensors (Basel) ; 23(2)2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36679665

RESUMO

Laser sensing and vision sensing smart canes can improve the convenience of travel for the visually impaired, but for the present, most of the system functions of laser sensing and vision sensing smart canes are still defective. Guide equipment and smart blind canes are introduced and classified first, and the smart blind canes based on vision sensing, laser sensing and laser vision sensing are investigated, respectively, and the research status of laser vision sensing smart blind canes is sorted out. The advantages and disadvantages of various laser vision sensing smart blind canes are summarized, especially the research development of laser vision fusion as the core of new smart canes. The future development prospects of laser vision sensing smart blind cane are overviewed, to boost the development of laser vision sensing smart blind cane, to provide safe and efficient travel guarantee for the visually impaired.


Assuntos
Bengala , Pessoas com Deficiência Visual , Humanos , Desenho de Equipamento , Cegueira
18.
J Biomech ; 146: 111426, 2023 01.
Artigo em Inglês | MEDLINE | ID: mdl-36608543

RESUMO

The cane is commonly prescribed for the elderly to maintain balance and enhance independent mobility. However, improper use of cane can increase the risk of falling. Understanding the characteristics of cane gait is critical for better establishing proper cane usage norms. The paper aimed to investigate effects of cane use on kinematics and kinetics of lower extremities in the elderly and the young to guide the development of adaptive cane gait. Twenty participants (10 elder and 10 young) were recruited and walked at a self-comfortable speed or with a cane in a two-point gait. The spatiotemporal gait parameters, hip/knee/ankle joint angles and ground reaction force (GRF) were statistically analyzed using MANOVAs to assess the effects of age and cane. Using the cane significantly decreased step length, cadence and speed and increased step time in both age groups. Age and cane had significant effects on ankle plantarflexion angle in initial swing phase (APA-ISw). In cane gait, the peaks of vertical GRF(V-GRF) and anterior-posterior GRF (AP-GRF) in bilateral lower extremities significantly decreased, and the troughs of right V-GRF significantly increased for both groups. These results suggest that using a cane does interfere with the natural gait of the user and insufficient ankle plantarflexion in initial swing phase (ISw) and reduced AP-GRF may be two key risk factors contributing to cane gait instability. Therefore, the users should consider actively increasing ankle plantarflexion in ISw to avoid deteriorating gait performance due to over-reliance on the cane.


Assuntos
Bengala , Extremidade Inferior , Humanos , Idoso , Fenômenos Biomecânicos , Cinética , Marcha , Caminhada , Articulação do Tornozelo
19.
Appl Biochem Biotechnol ; 195(5): 3406-3424, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-36598641

RESUMO

Cane molasses is a by-product of sugar industry. It is widely used in fermentation field, but pigment compounds affect its further application. In this study, nonpolar hyper-cross-linked adsorption resins (HCARs) were synthesized by pendent vinyl groups cross-linking reaction, and were applied to decolorization of molasses. The correlation between the structure and the decolorization performance of HCARs was studied, and the results showed that the Brunauer-Emmett-Teller (BET) surface area and the pore volume of the resin significantly increased to 574.4 m2·g-1 and 1.40 cm3·g-1 after the Friedel-Crafts alkylation reaction with a catalyst dosage of 2.25% at 343 K for 7 h. Furthermore, the decolorization rate of molasses by the HCAR was 74%, and recycle decolorization performance of the resin was stable. The adsorption kinetics results showed that the pseudo-second-order dynamic model could more realistically reflect the decolorization mechanism of molasses on HCARs, and liquid film diffusion is the main rate-limiting step. The results of fixed-bed experiments show that D-ST-DVB resin has a good decolorization effect and recycling ability. Therefore, it is a feasible strategy for the decolorization of molasses with nonpolar HCAR.


Assuntos
Melaço , Poliestirenos , Poliestirenos/química , Bengala , Fermentação
20.
Prep Biochem Biotechnol ; 53(9): 1043-1057, 2023 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36655700

RESUMO

Commercial production of lactic acid (LA) utilizes mostly glucose or lactose coupled with yeast extract (YE) as a supplement. With sugars, nitrogen, and vitamin supplementation being most of the LA production costs, the use of inexpensive molasses, a by-product of the sugar industry, can provide considerable cost savings. There are just a few publications on the production of LA from molasses; consequently, the present investigation was conducted using molasses supplemented with yeast extract. The research was done in a continuous-flow, high-cell-density (HCD) bioreactor with an external membrane microfiltration device for cell recycling. The system, run at 1 L with Lactobacillus delbrueckii NCIM 2025, produced a LA yield of 0.95-0.98 g/g from ∼100 g sugars/L when supplemented with 1 g/L YE. Dilution rates in the range of 0.04-0.36 h-1 resulted in volumetric lactic acid productivities in the range of 4.3-27.6 g/L h, which compares favorably with the highest values recorded in literature, for glucose in the presence of YE, which was as high as 30 g/L. The utilization of cane molasses has a significant impact on the economics of lactic acid production, as measured by a comparison of costs with commercial glucose.


Assuntos
Bengala , Melaço , Fermentação , Meios de Cultura , Ácido Láctico/metabolismo , Glucose
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