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1.
PLoS One ; 19(7): e0300643, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38954725

RESUMO

As most teleosts are unable to synthesize vitamin C, supplemental diets containing vitamin C diets play a crucial role in fish health. The aim of this study was to investigate the effect of dietary vitamin C on the intestinal enzyme activity and intestinal microbiota of silver pomfre (Pampus argenteus). Four experimental diets were supplemented with basic diets containing 300 mg of vitamin C/kg (group tjl3), 600 mg of vitamin C/kg (group tjl6), and 1200 mg of vitamin C/kg (group tjl12), as well as vitamin C-free supplemental basic diet (group tjl0), respectively. The four diets were fed to juvenile P. argenteus (average initial weight: 4.68 ± 0.93 g) for 6 weeks. The results showed that the activity of SOD (superoxide dismutase) and CAT (catalase) increased significantly while that of MDA (malondialdehyde) decreased significantly in group tjl3 compared to vitamin group tjl0. At the genus level, groups tjl0, tjl6, and tjl12 contained the same dominant microbial community, Stenotrophomonas, Photobacterium, and Vibrio, whereas group tjl3 was dominated by Stenotrophomonas, Delftia, and Bacteroides. Among the fish fed with a basic diet containing 300 mg of vitamin C/kg, the intestines exhibited a notable abundance of probiotic bacteria, including lactic acid bacteria (Lactobacillus) and Bacillus. The abundance of Aeromonas in groups tjl3 and tjl6 was lower than that of the vitamin C-free supplemental basic diet group, whereas Aeromonas was not detected in group tjl12. In addition, a causative agent of the disease outbreak in cultured P. argenteus, Photobacterium damselae subsp. Damselae (PDD) was the dominant microbiota community in groups tjl0, tjl6 and tjl12, whereas the abundance of PDD in group tjl3 was the lowest among the diets. Taken together, the diets supplied with vitamin C could influence the composition microbial community of P. argenteus. The low level of vitamin C (300 mg of vitamin C/kg per basic diet) supplementation could not only improve the antioxidant capacity but also resist the invasion of pathogenic bacteria.


Assuntos
Antioxidantes , Ácido Ascórbico , Suplementos Nutricionais , Microbioma Gastrointestinal , Animais , Ácido Ascórbico/farmacologia , Microbioma Gastrointestinal/efeitos dos fármacos , Antioxidantes/farmacologia , Antioxidantes/metabolismo , Perciformes/microbiologia , Ração Animal/análise , Superóxido Dismutase/metabolismo , Bactérias/efeitos dos fármacos , Bactérias/isolamento & purificação , Dieta/veterinária , Catalase/metabolismo
2.
ACS Appl Mater Interfaces ; 16(28): 36047-36062, 2024 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-38978477

RESUMO

Sepsis, a life-threatening condition caused by a dysregulated immune response to infection, leads to systemic inflammation, immune dysfunction, and multiorgan damage. Various oxidoreductases play a very important role in balancing oxidative stress and modulating the immune response, but they are stored inconveniently, environmentally unstable, and expensive. Herein, we develop multifunctional artificial enzymes, CeO2 and Au/CeO2 nanozymes, exhibiting five distinct enzyme-like activities, namely, superoxide dismutase, catalase, glutathione peroxidase, peroxidase, and oxidase. These artificial enzymes have been used for the biocatalytic treatment of sepsis via inhibiting inflammation and modulating immune responses. These nanozymes significantly reduce reactive oxygen species and proinflammatory cytokines, achieving multiorgan protection. Notably, CeO2 and Au/CeO2 nanozymes with enzyme-mimicking activities can be particularly effective in restoring immunosuppression and maintaining homeostasis. The redox nanozyme offers a promising dual-protective strategy against sepsis-induced inflammation and organ dysfunction, paving the way for biocatalytic-based immunotherapies for sepsis and related inflammatory diseases.


Assuntos
Cério , Ouro , Inflamação , Sepse , Sepse/tratamento farmacológico , Sepse/imunologia , Animais , Inflamação/tratamento farmacológico , Inflamação/imunologia , Ouro/química , Cério/química , Cério/uso terapêutico , Camundongos , Humanos , Espécies Reativas de Oxigênio/metabolismo , Catalase/metabolismo , Catalase/química , Citocinas/metabolismo
3.
Lipids Health Dis ; 23(1): 214, 2024 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-38982376

RESUMO

Omega-3 polyunsaturated fatty acids (n-3 PUFAs), mainly including α-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), possess antioxidant properties and play a crucial role in growth and development. However, the combined effects of ALA, EPA, and DHA at different concentrations have rarely been reported. This work explored the effects of EPA, ALA, and DHA on the viability and antioxidant capacity of mouse hepatocytes, with the objective of enhancing the antioxidant capacity. Within the appropriate concentration range, cell viability and the activity of glutathione S-transferase, superoxide dismutase, and catalase were increased, while the oxidation products of malondialdehyde and the level of intracellular reactive oxygen species were obviously reduced. Thus, oxidative stress was relieved, and cellular antioxidant levels were improved. Finally, response surface optimization was carried out for EPA, ALA, and DHA, and the model was established. The antioxidant capacity of the cells was highest at EPA, ALA, and DHA concentrations of 145.46, 405.05, and 551.52 µM, respectively. These findings lay the foundation for further exploration of the interactive mechanisms of n-3 PUFAs in the body, as well as their applications in nutraceutical food.


Assuntos
Antioxidantes , Sobrevivência Celular , Ácidos Docosa-Hexaenoicos , Ácido Eicosapentaenoico , Ácidos Graxos Ômega-3 , Hepatócitos , Estresse Oxidativo , Espécies Reativas de Oxigênio , Superóxido Dismutase , Animais , Camundongos , Hepatócitos/metabolismo , Hepatócitos/efeitos dos fármacos , Antioxidantes/farmacologia , Antioxidantes/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Ácidos Graxos Ômega-3/farmacologia , Ácido Eicosapentaenoico/farmacologia , Ácidos Docosa-Hexaenoicos/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Superóxido Dismutase/metabolismo , Catalase/metabolismo , Malondialdeído/metabolismo , Ácido alfa-Linolênico/farmacologia , Glutationa Transferase/metabolismo
4.
Proc Natl Acad Sci U S A ; 121(30): e2321972121, 2024 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-39008677

RESUMO

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection inhibits mitochondrial oxidative phosphorylation (OXPHOS) and elevates mitochondrial reactive oxygen species (ROS, mROS) which activates hypoxia-inducible factor-1alpha (HIF-1α), shifting metabolism toward glycolysis to drive viral biogenesis but also causing the release of mitochondrial DNA (mtDNA) and activation of innate immunity. To determine whether mitochondrially targeted antioxidants could mitigate these viral effects, we challenged mice expressing human angiotensin-converting enzyme 2 (ACE2) with SARS-CoV-2 and intervened using transgenic and pharmacological mitochondrially targeted catalytic antioxidants. Transgenic expression of mitochondrially targeted catalase (mCAT) or systemic treatment with EUK8 decreased weight loss, clinical severity, and circulating levels of mtDNA; as well as reduced lung levels of HIF-1α, viral proteins, and inflammatory cytokines. RNA-sequencing of infected lungs revealed that mCAT and Eukarion 8 (EUK8) up-regulated OXPHOS gene expression and down-regulated HIF-1α and its target genes as well as innate immune gene expression. These data demonstrate that SARS-CoV-2 pathology can be mitigated by catalytically reducing mROS, potentially providing a unique host-directed pharmacological therapy for COVID-19 which is not subject to viral mutational resistance.


Assuntos
Antioxidantes , COVID-19 , Camundongos Transgênicos , Mitocôndrias , Fosforilação Oxidativa , SARS-CoV-2 , Animais , Camundongos , COVID-19/virologia , COVID-19/metabolismo , COVID-19/imunologia , COVID-19/patologia , Antioxidantes/metabolismo , Antioxidantes/farmacologia , Mitocôndrias/metabolismo , Mitocôndrias/efeitos dos fármacos , SARS-CoV-2/efeitos dos fármacos , Fosforilação Oxidativa/efeitos dos fármacos , Humanos , Enzima de Conversão de Angiotensina 2/metabolismo , Enzima de Conversão de Angiotensina 2/genética , Pulmão/virologia , Pulmão/patologia , Pulmão/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , DNA Mitocondrial/genética , DNA Mitocondrial/metabolismo , Catalase/metabolismo , Catalase/genética , Tratamento Farmacológico da COVID-19 , Modelos Animais de Doenças , Imunidade Inata
5.
Harmful Algae ; 137: 102680, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-39003030

RESUMO

High levels of environmental H2O2 represent a threat to many freshwater bacterial species, including toxic-bloom-forming Microcystis aeruginosa, particularly under high-intensity light conditions. The highest extracellular catalase activity-possessing Pseudoduganella aquatica HC52 was chosen among 36 culturable symbiotic isolates from the phycosphere in freshly collected M. aeruginosa cells. A zymogram for catalase activity revealed the presence of only one extracellular catalase despite the four putative catalase genes (katA1, katA2, katE, and srpA) identified in the newly sequenced genome (∼6.8 Mb) of P. aquatica HC52. Analysis of secreted catalase using liquid chromatography-tandem mass spectrometry was identified as KatA1, which lacks a typical signal peptide, although the underlying mechanism for its secretion is unknown. The expression of secreted KatA1 appeared to be induced in the presence of H2O2. Proteomic analysis also confirmed the presence of KatA1 inside the outer membrane vesicles secreted by P. aquatica HC52 following exposure to H2O2. High light intensities (> 100 µmol m-2 s-1) are known to kill catalase-less axenic M. aeruginosa cells, but the present study found that the presence of P. aquatica cells supported the growth of M. aeruginosa, while the extracellular catalases in supernatant or purified form also sustained the growth of M. aeruginosa under the same conditions. Our results suggest that the extracellular catalase secreted by P. aquatica HC52 enhances the tolerance of M. aeruginosa to H2O2, thus promoting the formation of M. aeruginosa blooms under high light intensities.


Assuntos
Proteínas de Bactérias , Catalase , Peróxido de Hidrogênio , Microcystis , Peróxido de Hidrogênio/metabolismo , Microcystis/genética , Catalase/metabolismo , Catalase/genética , Proteínas de Bactérias/metabolismo , Proteínas de Bactérias/genética
6.
J Nanobiotechnology ; 22(1): 412, 2024 Jul 12.
Artigo em Inglês | MEDLINE | ID: mdl-38997713

RESUMO

The senescence of nucleus pulposus (NP) cells (NPCs), which is induced by the anomalous accumulation of reactive oxygen species (ROS), is a major cause of intervertebral disc degeneration (IVDD). In this research, glutathione-doped carbon dots (GSH-CDs), which are novel carbon dot antioxidant nanozymes, were successfully constructed to remove large amounts of ROS for the maintenance of NP tissue at the physical redox level. After significantly scavenging endogenous ROS via exerting antioxidant activities, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and total antioxidant capacity, GSH-CDs with good biocompatibility have been demonstrated to effectively improve mitochondrial dysfunction and rescue NPCs from senescence, catabolism, and inflammatory factors in vivo and in vitro. In vivo imaging data and histomorphological indicators, such as the disc height index (DHI) and Pfirrmann grade, demonstrated prominent improvements in the progression of IVDD after the topical application of GSH-CDs. In summary, this study investigated the GSH-CDs nanozyme, which possesses excellent potential to inhibit the senescence of NPCs with mitochondrial lesions induced by the excessive accumulation of ROS and improve the progression of IVDD, providing potential therapeutic options for clinical treatment.


Assuntos
Carbono , Glutationa , Degeneração do Disco Intervertebral , Núcleo Pulposo , Estresse Oxidativo , Espécies Reativas de Oxigênio , Degeneração do Disco Intervertebral/tratamento farmacológico , Degeneração do Disco Intervertebral/metabolismo , Degeneração do Disco Intervertebral/patologia , Núcleo Pulposo/metabolismo , Núcleo Pulposo/efeitos dos fármacos , Núcleo Pulposo/patologia , Animais , Estresse Oxidativo/efeitos dos fármacos , Carbono/química , Carbono/farmacologia , Glutationa/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Pontos Quânticos/química , Antioxidantes/farmacologia , Masculino , Senescência Celular/efeitos dos fármacos , Células Cultivadas , Humanos , Mitocôndrias/metabolismo , Mitocôndrias/efeitos dos fármacos , Microambiente Celular/efeitos dos fármacos , Catalase/metabolismo , Catalase/farmacologia , Superóxido Dismutase/metabolismo
7.
Molecules ; 29(13)2024 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-38999063

RESUMO

As part of the multifaceted strategies developed to shape the common environmental policy, considerable attention is now being paid to assessing the degree of environmental degradation in soil under xenobiotic pressure. Bisphenol A (BPA) has only been marginally investigated in this ecosystem context. Therefore, research was carried out to determine the biochemical properties of soils contaminated with BPA at two levels of contamination: 500 mg and 1000 mg BPA kg-1 d.m. of soil. Reliable biochemical indicators of soil changes, whose activity was determined in the pot experiment conducted, were used: dehydrogenases, catalase, urease, acid phosphatase, alkaline phosphatase, arylsulfatase, and ß-glucosidase. Using the definition of soil health as the ability to promote plant growth, the influence of BPA on the growth and development of Zea mays, a plant used for energy production, was also tested. As well as the biomass of aerial parts and roots, the leaf greenness index (SPAD) of Zea mays was also assessed. A key aspect of the research was to identify those of the six remediating substances-molecular sieve, zeolite, sepiolite, starch, grass compost, and fermented bark-whose use could become common practice in both environmental protection and agriculture. Exposure to BPA revealed the highest sensitivity of dehydrogenases, urease, and acid phosphatase and the lowest sensitivity of alkaline phosphatase and catalase to this phenolic compound. The enzyme response generated a reduction in the biochemical fertility index (BA21) of 64% (500 mg BPA) and 70% (1000 mg BPA kg-1 d.m. of soil). The toxicity of BPA led to a drastic reduction in root biomass and consequently in the aerial parts of Zea mays. Compost and molecular sieve proved to be the most effective in mitigating the negative effect of the xenobiotic on the parameters discussed. The results obtained are the first research step in the search for further substances with bioremediation potential against both soil and plants under BPA pressure.


Assuntos
Fosfatase Ácida , Compostos Benzidrílicos , Fenóis , Poluentes do Solo , Solo , Zea mays , Fenóis/química , Compostos Benzidrílicos/química , Poluentes do Solo/química , Zea mays/química , Solo/química , Fosfatase Ácida/metabolismo , Arilsulfatases/metabolismo , Fosfatase Alcalina/metabolismo , Zeolitas/química , Oxirredutases/metabolismo , Urease/metabolismo , Catalase/metabolismo , Biodegradação Ambiental , Silicatos de Magnésio/química , Amido/química , beta-Glucosidase/metabolismo , Compostagem/métodos
8.
Clin Biochem ; 130: 110788, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38969053

RESUMO

Oxidative stress (OS) results from the imbalance between the production of reactive oxygen species and the body's antioxidant mechanisms and is associated with various diseases, including depression. Antioxidants protect cells by neutralizing free radicals and include enzymatic components such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), and glutathione S-transferase (GST). The concentration of these biomarkers can quantify OS. This research aimed to gather available information published in the last ten years about the concentration of enzymatic OS biomarkers in samples from patients with depressive disorders. METHOD: A systematic review was conducted following the PRISMA guidelines, including original scientific articles that evaluated enzymatic OS biomarkers in participants with depressive disorders, using the keywords and boolean operators "superoxide dismutase" OR "catalase" OR "glutathione" AND "depress*" in the databases PubMed, SAGE Journals, DOAJ, Scielo, Dialnet, and Redalyc. RESULTS: The initial search showed 614 results, with only 28 articles meeting the selection criteria. It was observed that all evaluated oxidative stress enzymatic markers showed a significant increase or decrease in patients with depressive disorders, due to a wide variability in the depressive disorders studied, the type of biological sample analyzed, and the techniques used. CONCLUSION: There is evidence of the relationship between enzymatic OS biomarkers and depressive disorders, but additional studies are needed to clarify the nature of this relationship, particularly considering the different types of depressive disorders.


Assuntos
Biomarcadores , Transtorno Depressivo , Estresse Oxidativo , Humanos , Antioxidantes/metabolismo , Biomarcadores/metabolismo , Catalase/metabolismo , Catalase/sangue , Transtorno Depressivo/metabolismo , Glutationa Peroxidase/metabolismo , Glutationa Redutase/metabolismo , Glutationa Transferase/metabolismo , Superóxido Dismutase/metabolismo
9.
Mol Biol Rep ; 51(1): 798, 2024 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-39002019

RESUMO

BACKGROUND: Hexavalent chromium (CrVI) is known to be a potentially hepatotoxic and nephrotoxic contaminant in humans and other animals, whose toxicity is associated with oxidative stress and inflammation. The aim of this study was to evaluate the potential protective effect of chlorogenic acid (CGA), which has known anti-inflammatory and antioxidant effects, on potassium dichromate (PDC)-induced acute hepatotoxicity and nephrotoxicity in rats. METHODS AND RESULTS: Thirty-six Wistar albino rats were treated with CGA (10, 20, or 40 mg/kg, intraperitoneally) and/or PDC (15 mg/kg/day, intraperitoneally) as a single dose. Serum, liver, and kidney tissues were examined biochemically, histopathologically, and immunohistochemically. Compared to the control group, a significant increase in interleukin-6 (IL-6) levels and a significant decrease in serum and renal reduced glutathione (GSH) levels, liver catalase (CAT), tumour necrosis factor-alpha (TNF-α), and interleukin 1ß (IL-1ß) levels were observed in the PDC group. The administration of PDC led to histopathological and immunohistochemical changes in rat liver and kidney tissues. With the administration of CGA, especially at the 10 mg/kg dosage, the above-mentioned parameters approached normal levels. CONCLUSIONS: CGA had antioxidant and anti-inflammatory effects that alleviated PDC-induced acute hepato- and nephrotoxicity.


Assuntos
Antioxidantes , Ácido Clorogênico , Rim , Fígado , NF-kappa B , Estresse Oxidativo , Dicromato de Potássio , Ratos Wistar , Transdução de Sinais , Animais , Dicromato de Potássio/toxicidade , Ácido Clorogênico/farmacologia , Ratos , Transdução de Sinais/efeitos dos fármacos , NF-kappa B/metabolismo , Rim/efeitos dos fármacos , Rim/metabolismo , Rim/patologia , Estresse Oxidativo/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Masculino , Antioxidantes/farmacologia , Antioxidantes/metabolismo , Interleucina-6/metabolismo , Glutationa/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , Interleucina-1beta/metabolismo , Catalase/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico
10.
Nat Commun ; 15(1): 5908, 2024 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-39003316

RESUMO

Although Prussian blue nanozymes (PBNZ) are widely applied in various fields, their catalytic mechanisms remain elusive. Here, we investigate the long-term catalytic performance of PBNZ as peroxidase (POD) and catalase (CAT) mimetics to elucidate their lifespan and underlying mechanisms. Unlike our previously reported Fe3O4 nanozymes, which exhibit depletable POD-like activity, the POD and CAT-like activities of PBNZ not only persist but slightly enhance over prolonged catalysis. We demonstrate that the irreversible oxidation of PBNZ significantly promotes catalysis, leading to self-increasing catalytic activities. The catalytic process of the pre-oxidized PBNZ can be initiated through either the conduction band pathway or the valence band pathway. In summary, we reveal that PBNZ follows a dual-path electron transfer mechanism during the POD and CAT-like catalysis, offering the advantage of a long service life.


Assuntos
Catalase , Ferrocianetos , Oxirredução , Peroxidase , Ferrocianetos/química , Catálise , Catalase/química , Catalase/metabolismo , Peroxidase/metabolismo , Peroxidase/química , Peróxido de Hidrogênio/química , Peróxido de Hidrogênio/metabolismo , Nanoestruturas/química
11.
Biosens Bioelectron ; 262: 116568, 2024 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-39003919

RESUMO

In this study, a dual-mode biosensor based on the heterojunction of Cu2O@Cu2S/D-TA COF was constructed for ultra-sensitive detection of Hg2+ using both photoelectrochemical and electrochemical approaches. Briefly, a 2D ultra-thin covalent organic framework film (D-TA COF film) with excellent photoelectrochemical signals was prepared on ITO surfaces through an in situ growth method. Subsequently, the probe H1 was immobilized onto the biosensor via Au-S bonds. In the presence of Hg2+, the formation of T-Hg2+-T complexes triggered hybridization chain reactions (HCR), leading to the attachment of abundant Cu2O@Cu2S probes onto the biosensor. As a p-type semiconductor, Cu2O@Cu2S could form a heterojunction with the underlying D-TA COF films. Meanwhile, it exhibited catalase-like activity, and the O2 produced by its catalytic decomposition of H2O2 can interact with the D-TA COF films, thus achieving double amplification of the photocurrent signal. Benefiting from the excellent and inherent Cu2+/Cu+ redox pairs of Cu2O@Cu2S, satisfactory differential pulse voltammetry (DPV) signals were obtained. As expected, the dual-mode biosensor was realized with wider linear ranges and low detection limits. Additionally, the analytical performance for Hg2+ in real water samples was excellent. Briefly, this suggested approach offers a facile and highly efficient modality for monitoring heavy metal ions in aquatic environments.


Assuntos
Técnicas Biossensoriais , Catalase , Cobre , Técnicas Eletroquímicas , Limite de Detecção , Mercúrio , Estruturas Metalorgânicas , Técnicas Biossensoriais/métodos , Cobre/química , Mercúrio/análise , Mercúrio/química , Técnicas Eletroquímicas/métodos , Estruturas Metalorgânicas/química , Catalase/química , Poluentes Químicos da Água/análise , Peróxido de Hidrogênio/química , Sulfetos
12.
J Mol Neurosci ; 74(3): 72, 2024 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-39042258

RESUMO

Antioxidant-rich supplementation plays an essential role in the function of mammals' central nervous system. However, no research has documented the effect of berberine (BER) supplementation on the cerebrocerebellar function of prepubertal rats. The present study was designed to investigate the impact of BER supplementation on neurochemical and behavioural changes in prepubertal male rats. Five groups (90 ± 5 g, n = 7 each) of experimental rats were orally treated with corn oil or different doses of BER (25, 50, 100, and 200 mg/kg bw) from the 28th at 68 post-natal days. On the 69 days of life, animals underwent behavioural assessment in the open field, hanging wire, and negative geotaxis tests. The result revealed that BER administration improved locomotive and motor behaviour by increasing distance travelled, line crossings, average speed, time mobile, and absolute turn angle in open field test and decrease in time to re-orient on an incline plane, a decrease in immobility time relative to the untreated control. Furthermore, BER supplementation increased (p < 0.05) antioxidant enzyme activities such as SOD, CAT, GPx, GSH, and TSH and prevented increases (p < 0.05) in oxidative and inflammatory levels as indicated by decreases in RONS, LPO, XO, carbonyl protein, NO, MPO, and TNF-α compared to the untreated control. BER-treated animals a lessened number of dark-stained Nissl cells compared to the untreated control rats. Our findings revealed that BER minimised neuronal degeneration and lesions, improved animal behaviour, and suppressed oxidative and inflammatory mediators, which may probably occur through its agonistic effect on PPAR-α, PPAR-δ, and PPAR-γ - essential proteins known to resolve inflammation and modulate redox signalling towards antioxidant function.


Assuntos
Antioxidantes , Berberina , Ratos Wistar , Animais , Masculino , Ratos , Berberina/farmacologia , Berberina/administração & dosagem , Antioxidantes/farmacologia , Antioxidantes/metabolismo , Cerebelo/metabolismo , Cerebelo/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Atividade Motora/efeitos dos fármacos , Superóxido Dismutase/metabolismo , Glutationa/metabolismo , Catalase/metabolismo , Glutationa Peroxidase/metabolismo
13.
Mol Biol Rep ; 51(1): 833, 2024 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-39039363

RESUMO

BACKGROUND: Hepatotoxicity associated with methotrexate (MTX) is mainly due to disruption of redox balance and development of oxidative injury to hepatocytes. Melatonin (MLT) is a potent antioxidant and regulates wide range of biological functions, processes and utilized as adjuvant for number of medical applications. The current study investigated the mitigating effect of MLT on the MTX-induced hepatotoxicity. METHODS AND RESULTS: Adult male rats received MLT (25 mg/kg, orally) for seven days flowed by single injection of MTX (20 mg/kg, ip) then treat with MLT continued for additional 7 days. The present result showed MLT treatment mitigated histopathological changes in the liver that associated with normalization of ALT and AST activity as well as bilirubin, albumin and alfa-fetoprotein levels in serum of MLT + MTX-treated rat to comparable control level. MLT treatment significantly reduced MDA content and myeloperoxidase activity while enhanced the activity of superoxide dismutase, catalase and glutathione content in the liver indicating the empowerment of the antioxidant status. Amelioration of MLT-induced oxidative stress resulted in a reduction in the inflammatory response due to antioxidant restoration and inhibited apoptosis indicated by downregulation of caspase-3 expression. The replenishment of antioxidant content powers the defense system of the hepatocytes. As a result, apoptosis is reduced which might be due to the ability of MLT protect DNA integrity thus maintaining hepatocyte functions and structure. Consequently, liver histology was protected. CONCLUSIONS: In summary, MLT modulates liver function and structure by orchestrating linked processes, including redox balance, inflammatory response, suppression of caspase-3, and DNA damage.


Assuntos
Antioxidantes , Apoptose , Doença Hepática Induzida por Substâncias e Drogas , Hepatócitos , Fígado , Melatonina , Metotrexato , Estresse Oxidativo , Animais , Metotrexato/efeitos adversos , Metotrexato/toxicidade , Melatonina/farmacologia , Ratos , Masculino , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Estresse Oxidativo/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Antioxidantes/farmacologia , Antioxidantes/metabolismo , Apoptose/efeitos dos fármacos , Hepatócitos/efeitos dos fármacos , Hepatócitos/metabolismo , Superóxido Dismutase/metabolismo , Glutationa/metabolismo , Catalase/metabolismo
14.
Subcell Biochem ; 104: 33-47, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38963482

RESUMO

Catalases are essential enzymes for removal of hydrogen peroxide, enabling aerobic and anaerobic metabolism in an oxygenated atmosphere. Monofunctional heme catalases, catalase-peroxidases, and manganese catalases, evolved independently more than two billion years ago, constituting a classic example of convergent evolution. Herein, the diversity of catalase sequences is analyzed through sequence similarity networks, providing the context for sequence distribution of major catalase families, and showing that many divergent catalase families remain to be experimentally studied.


Assuntos
Catalase , Evolução Molecular , Catalase/química , Catalase/genética , Catalase/metabolismo , Humanos , Animais , Peróxido de Hidrogênio/metabolismo , Peróxido de Hidrogênio/química , Heme/química , Heme/metabolismo
15.
BMC Biol ; 22(1): 149, 2024 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-38965504

RESUMO

BACKGROUND: Organisms frequently experience environmental stresses that occur in predictable patterns and combinations. For wild Saccharomyces cerevisiae yeast growing in natural environments, cells may experience high osmotic stress when they first enter broken fruit, followed by high ethanol levels during fermentation, and then finally high levels of oxidative stress resulting from respiration of ethanol. Yeast have adapted to these patterns by evolving sophisticated "cross protection" mechanisms, where mild 'primary' doses of one stress can enhance tolerance to severe doses of a different 'secondary' stress. For example, in many yeast strains, mild osmotic or mild ethanol stresses cross protect against severe oxidative stress, which likely reflects an anticipatory response important for high fitness in nature. RESULTS: During the course of genetic mapping studies aimed at understanding the mechanisms underlying natural variation in ethanol-induced cross protection against H2O2, we found that a key H2O2 scavenging enzyme, cytosolic catalase T (Ctt1p), was absolutely essential for cross protection in a wild oak strain. This suggested the absence of other compensatory mechanisms for acquiring H2O2 resistance in that strain background under those conditions. In this study, we found surprising heterogeneity across diverse yeast strains in whether CTT1 function was fully necessary for acquired H2O2 resistance. Some strains exhibited partial dispensability of CTT1 when ethanol and/or salt were used as mild stressors, suggesting that compensatory peroxidases may play a role in acquired stress resistance in certain genetic backgrounds. We leveraged global transcriptional responses to ethanol and salt stresses in strains with different levels of CTT1 dispensability, allowing us to identify possible regulators of these alternative peroxidases and acquired stress resistance in general. CONCLUSIONS: Ultimately, this study highlights how superficially similar traits can have different underlying molecular foundations and provides a framework for understanding the diversity and regulation of stress defense mechanisms.


Assuntos
Peróxido de Hidrogênio , Saccharomyces cerevisiae , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/fisiologia , Saccharomyces cerevisiae/efeitos dos fármacos , Peróxido de Hidrogênio/farmacologia , Peróxido de Hidrogênio/metabolismo , Etanol/farmacologia , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Estresse Fisiológico/genética , Estresse Fisiológico/efeitos dos fármacos , Pressão Osmótica , Catalase/metabolismo , Catalase/genética , Variação Genética
16.
Inorg Chem ; 63(29): 13681-13688, 2024 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-38982342

RESUMO

Mimicking enzymatic processes carried out by natural enzymes, which are highly efficient biocatalysts with key roles in living organisms, attracts much interest but constitutes a synthetic challenge. Biological metal-organic frameworks (bioMOFs) are potential candidates to be enzyme catalysis mimics, as they offer the possibility to combine biometals and biomolecules into open-framework porous structures capable of simulating the catalytic pockets of enzymes. In this work, we first study the catalase activity of a previously reported bioMOF, derived from the amino acid L-serine, with formula {CaIICuII6[(S,S)-serimox]3(OH)2(H2O)} · 39H2O (1) (serimox = bis[(S)-serine]oxalyl diamide), which is indeed capable to mimic catalase enzymes, in charge of preventing cell oxidative damage by decomposing, efficiently, hydrogen peroxide to water and oxygen (2H2O2 → 2 H2O + O2). With these results in hand, we then prepared a new multivariate bioMOF (MTV-bioMOF) that combines two different types of bioligands derived from L-serine and L-histidine amino acids with formula CaIICuII6[(S,S)-serimox]2[(S,S)-hismox]1(OH)2(H2O)}·27H2O (2) (hismox = bis[(S)-histidine]oxalyl diamide ligand). MTV-bioMOF 2 outperforms 1 degrading hydrogen peroxide, confirming the importance of the amino acid residue from the histidine amino acid acting as a nucleophile in the catalase degradation mechanism. Despite displaying a more modest catalytic behavior than other reported MOF composites, in which the catalase enzyme is immobilized inside the MOF, this work represents the first example of a MOF in which an attempt is made to replicate the active center of the catalase enzyme with its constituent elements and is capable of moderate catalytic activity.


Assuntos
Materiais Biomiméticos , Catalase , Domínio Catalítico , Estruturas Metalorgânicas , Estruturas Metalorgânicas/química , Catalase/química , Catalase/metabolismo , Materiais Biomiméticos/química , Peróxido de Hidrogênio/química , Peróxido de Hidrogênio/metabolismo , Modelos Moleculares
17.
Biomed Res Int ; 2024: 2222098, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39015602

RESUMO

In this study, we aimed to isolate and purify catalase from human blood erythrocytes by using a newly synthesized affinity gel. The synthesized ω-amino hexyl agarose-1,2,3-triazole-5-carboxylic acid affinity gel was analyzed by FT-IR. Then, different buffer, pH, and ionic strength parameters were optimized to determine the equilibration, washing, and elution buffer conditions. The catalase was purified from human blood erythrocytes with a specific activity of 45.58 EU/mg, purification fold of 529.50, and a yield of 0.416% using the synthesized new affinity gel. The purity and molecular weight of the enzyme were analyzed by SDS-PAGE, and a single band at 60 kDa was observed for catalase. The optimum reaction temperature of the catalase was found to be 30°C, while the thermal stability temperature was 60°C. The Km and Vmax of the enzyme for hydrogen peroxide were calculated at 0.125 mM and 2500 U mL-1, respectively.


Assuntos
Catalase , Cromatografia de Afinidade , Eritrócitos , Humanos , Catalase/química , Catalase/isolamento & purificação , Catalase/metabolismo , Eritrócitos/enzimologia , Cromatografia de Afinidade/métodos , Concentração de Íons de Hidrogênio , Temperatura , Estabilidade Enzimática , Cinética , Peróxido de Hidrogênio/química , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Peso Molecular
18.
Mar Pollut Bull ; 205: 116683, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38972218

RESUMO

This study examines the combined effects of polyethylene microplastics (PE-MP) and copper (Cu2+) on the immune and oxidative response of Litopenaeus vannamei. PE-MP adsorbed with Cu2+ at 2.3, 6.8, and 16.8 ng (g shrimp)-1) were injected into L. vannamei. Over 14 days, survival rates were monitored, and immune and oxidative stress parameters were assessed. The results showed that combined exposure to PE-MP and Cu2+ significantly reduced the survival rate and decreased total haemocyte count. Immune-related parameters (phagocytic rate, phenoloxidase and superoxide dismutase (SOD)) and antioxidant-related parameters (SOD, catalase and glutathione peroxidase mRNA and enzyme) also decreased, while respiratory burst activity significantly increased, indicating immune and antioxidant system disruption. Additionally, there was a significant increase in oxidative stress, as measured by malondialdehyde levels. Histopathological analysis revealed severe muscle, hepatopancreas, and gill damage. These results suggest that simultaneous exposure to PE-MP and Cu2+ poses greater health risks to white shrimp.


Assuntos
Cobre , Microplásticos , Estresse Oxidativo , Penaeidae , Polietileno , Poluentes Químicos da Água , Animais , Penaeidae/efeitos dos fármacos , Cobre/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Polietileno/toxicidade , Microplásticos/toxicidade , Superóxido Dismutase/metabolismo , Brânquias/efeitos dos fármacos , Catalase/metabolismo , Glutationa Peroxidase/metabolismo
19.
Sci Rep ; 14(1): 16427, 2024 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-39013912

RESUMO

The ecotoxicological consequences of azoxystrobin on land snails have not yet been addressed. Therefore, the present study aims to provide novel data on the threat of a commercial grade azoxystrobin (AMISTAR) at two environmentally relevant concentrations (0.3 µg/ml) and tenfold (3 µg/ml) on the model species, Theba pisana by physiological, biochemical, and histopathological markers for 28 days. Our results showed a reduction in animal food consumption and growth due to exposure to both azoxystrobin concentrations. It also induced oxidative stress and led to a significant decrease in lipid peroxidation (LPO) levels after 7 days of exposure, while the opposite effect occurred after 28 days. Except for the 7-day exposure, all treated snails had significantly reduced glutathione (GSH) content and increased catalase (CAT) activity at all-time intervals. Glutathione peroxidase (GPx), glutathione-S-transferase (GST) activities, and protein content (PC) were elevated in treated snails at all-time intervals. Moreover, alterations in acetylcholinesterase (AChE) activity between a decrease and an increase were noticed. Additionally, azoxystrobin exerted changes in T. pisana hepatopancreas architecture. Our study suggests that azoxystrobin may have negative ecological consequences for T. pisana and highlights its potential risks to the natural environment.


Assuntos
Fungicidas Industriais , Glutationa , Metacrilatos , Estresse Oxidativo , Pirimidinas , Caramujos , Estrobilurinas , Animais , Estrobilurinas/toxicidade , Pirimidinas/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Fungicidas Industriais/toxicidade , Metacrilatos/toxicidade , Caramujos/efeitos dos fármacos , Caramujos/metabolismo , Glutationa/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Glutationa Transferase/metabolismo , Acetilcolinesterase/metabolismo , Ecotoxicologia , Catalase/metabolismo , Glutationa Peroxidase/metabolismo
20.
Sci Rep ; 14(1): 16425, 2024 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-39014067

RESUMO

Physalis alkekengi L. is a valuable medicinal plant from the Solanaceae family and has multiple therapeutic applications. This study aimed to develop an optimized protocol for callogenesis in P. alkekengi to obtain friable calluses with high biomass. The effect of different concentrations of picloram, casein hydrolysate (CH), basal media (Murashige and Skoog (MS) and Gamborg (B5)), and static magnetic field (SMF) were investigated on the callus induction and growth, signaling molecules, and enzymatic and non-enzymatic antioxidants. Results showed that CH (200 mgL-1) and SMF4 mT for 90 min increased callus induction and fresh weight in P. alkekengi, while different concentrations of picloram reduced callogenesis. Hypocotyl explants showed various callogenesis and metabolic responses depending on the basal medium type. The 2B5 medium supplied with CH 200 (mgL-1) induced friable and cream calluses with high biomass (0.62 g) compared to the MS medium (control). The maximum activity of superoxide dismutase and catalase activities was identified in the 2B5 medium and peroxidase in the 2MS medium. The highest total phenolic (129.44 µg g-1DW) content and phenylalanine-ammonia lyase activity were obtained in the 2MS medium, and total withanolides (49.86 µg g-1DW) and DPPH radical scavenging activity were observed in the 2B5 medium. The 2MS medium boosted the hydrogen peroxide and nitric oxide levels, while their contents alleviated in the 2B5 medium, although these parameters were higher than the control. The findings of this study suggest that an effective protocol for successful callogenesis in P. alkekengi and the nutrient composition of culture medium by affecting the level of signaling molecules can control the antioxidant defense system and callus growth.


Assuntos
Antioxidantes , Meios de Cultura , Óxido Nítrico , Physalis , Physalis/metabolismo , Óxido Nítrico/metabolismo , Antioxidantes/metabolismo , Meios de Cultura/química , Metabolismo Secundário , Biomassa , Superóxido Dismutase/metabolismo , Catalase/metabolismo
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