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1.
Inflammopharmacology ; 31(5): 2631-2640, 2023 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-37420144

RESUMO

Licania rigida Benth., a Brazilian endemic plant, has been traditionally used for treating inflammation and stomach pain. This work investigates the anti-inflammatory and gastroprotective activities of the ethanolic extract from L. rigida seeds (EELr) by in vitro and in vivo methods. The phytochemical profile was determined and the in vitro antioxidant activity was investigated by radical scavenging and thiobarbituric acid reactive substances methods. The ovalbumin denaturation method was used with sodium diclofenac as standard for the in vitro anti-inflammatory activity assessment. Acetylsalicylic acid was used to induce gastric ulcers in male mice and then to evaluate the preventive and therapeutic gastroprotective effect of EELr, using omeprazole as the reference drug. The extract exhibited relevant amount of phenolic compounds and flavonoids, in particular, demonstrating in vitro antioxidant capacity. EELr was able to inhibit almost 60% of ovalbumin denaturation at a concentration considered low. It also prevented the decrease of biochemical markers for oxidative stress such as superoxide dismutase (SOD) and reduced glutathione (GSH) in the stomach and SOD and catalase (CAT) in the liver. EELr also significantly decreased the number of lesions as well as reduced the ulcerated area when used as therapy. The observed effect may be due to its phenolic compounds, such as chlorogenic acid, caffeic acid and tannins, as previously reported. EELr is a potential source of compounds with anti-inflammatory activity, protects the liver from oxidative damage and improves healing of aspirin-induced ulcers. This work contributes to the knowledge of L. rigida species.


Assuntos
Antiulcerosos , Chrysobalanaceae , Úlcera Gástrica , Ratos , Camundongos , Animais , Extratos Vegetais/uso terapêutico , Úlcera Gástrica/induzido quimicamente , Úlcera Gástrica/tratamento farmacológico , Fitoterapia , Chrysobalanaceae/química , Ovalbumina/farmacologia , Ratos Wistar , Antiulcerosos/farmacologia , Anti-Inflamatórios/uso terapêutico , Antioxidantes/farmacologia , Antioxidantes/uso terapêutico , Etanol/química , Aspirina/farmacologia , Sementes , Superóxido Dismutase , Mucosa Gástrica
2.
BMC Complement Med Ther ; 23(1): 153, 2023 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-37165388

RESUMO

BACKGROUND: Natural products are an important source of bioproducts with pharmacological properties. Here we investigate the components of leaves from M. tomentosa Benth. (Fritsch) (Chrysobalanaceae) and its effects on bacterial cell growth, biofilm production and macrophage activity. METHODS: The effect of the different leaf extracts against bacterial cell growth was performed using the microdilution method. The most active extract was analyzed by mass spectrometry, and its effect on bacterial biofilm production was evaluated on polystyrene plates. The extract effect on macrophage activity was tested in the RAW264.7 cell line, which was stimulated with different concentrations of the extract in the presence or absence of LPS. RESULTS: We show that the ethyl acetate (EtOAc) extract was the most effective against bacterial cell growth. EtOAc extract DI-ESI (-)MSn analysis showed the presence of a glycosylated flavonoid tentatively assigned as myricetin 3-O-xylosyl-rhamnoside (MW 596). Also, the EtOAc extract increased biofilm formation by S. aureus and inhibited cytokine and NO production induced by LPS in RAW macrophages. CONCLUSION: M. tomentosa flavonoid-enriched EtOAc extract presented a bactericidal and anti-inflammatory pharmacological potential.


Assuntos
Chrysobalanaceae , Flavonoides , Flavonoides/farmacologia , Extratos Vegetais/química , Staphylococcus aureus , Lipopolissacarídeos/farmacologia , Antibacterianos/farmacologia , Anti-Inflamatórios/farmacologia , Bactérias
3.
Bioorg Chem ; 138: 106614, 2023 09.
Artigo em Inglês | MEDLINE | ID: mdl-37216893

RESUMO

The inflammatory response is a vital mechanism for repairing damage induced by aberrant health states or external insults; however, persistent activation can be linked to numerous chronic diseases. The nuclear factor kappa ß (NF-κB) inflammatory pathway and its associated mediators have emerged as critical targets for therapeutic interventions aimed at modulating inflammation, necessitating ongoing drug development. Previous studies have reported the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α), but the phytoconstituents and mechanisms of action remained elusive. The primary objective of this study was to elucidate the phytochemical composition of P. excelsa stem bark and its role in the mechanisms underpinning its biological activity. Two compounds were detected via HPLC-DAD-ESI(Ion Trap)-MS2 analysis. The predominant compound was isolated and identified as naringenin-8-sulphonate (1), while the identity of the second compound (compound 2) could not be determined. Both compound 1 and the extract were assessed for anti-inflammatory properties using a cell-based inflammation model, in which THP-1-derived macrophages were stimulated with LPS to examine the treatments' effects on various stages of the NF-κB pathway. Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1ß) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3). To explore the influence of sulphation on the anti-inflammatory properties of naringenin derivatives, we synthesized naringenin-4'-O-sulphate (4) and naringenin-7-O-sulphate (5) and evaluated their anti-inflammatory effects. Naringenin derivatives 4 and 5 did not display potent anti-inflammatory activities; however, compound 4 reduced IL-1ß production, and compound 5 diminished p65 translocation, with both exhibiting the capacity to inhibit TNF-α and IL-6 production. Collectively, the findings demonstrated that the P. excelsa extract was more efficacious than all tested compounds, while providing insights into the role of sulphation in the anti-inflammatory activity of naringenin derivatives.


Assuntos
Chrysobalanaceae , NF-kappa B , Humanos , NF-kappa B/metabolismo , Interleucina-6/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , Chrysobalanaceae/metabolismo , Casca de Planta/metabolismo , Anti-Inflamatórios/uso terapêutico , Inflamação/metabolismo , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Lipopolissacarídeos/farmacologia
4.
Molecules ; 27(19)2022 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-36234829

RESUMO

Licania rigida Benth has been evaluated as an alternative drug to treat diseases associated with inflammatory processes. This study evaluated the anti-inflammatory effects of aqueous and hydroalcoholic leaf extracts of L. rigida with inflammation induced by lipopolysaccharides in in vitro and in vivo inflammation models. The phytochemical profile of the extracts, analyzed by ultra-fast liquid chromatography coupled with tandem mass spectrometry, revealed the presence of gallic and ellagic acids in both extracts, whereas isovitexin, ferulate, bulky amino acids (e.g., phenylalanine), pheophorbide, lactic acid, and pyridoxine were detected in the hydroalcoholic extract. The extracts displayed the ability to modulate in vitro and in vivo inflammatory responses, reducing approximately 50% of pro-inflammatory cytokine secretion (TNF-α, IL-1ß, and IL-6), and inhibiting both NO production and leukocyte migration by approximately 30 and 40% at 100 and 500 µg/mL, respectively. Overall, the results highlight and identify, for the first time, the ability of L. rigida leaf extract to modulate inflammatory processes. These data suggest that the leaf extracts of this plant have potential in the development of herbal formulations for the treatment of inflammation.


Assuntos
Chrysobalanaceae , Aminoácidos , Anti-Inflamatórios/uso terapêutico , Inflamação/induzido quimicamente , Inflamação/tratamento farmacológico , Inflamação/metabolismo , Interleucina-6 , Ácido Láctico/efeitos adversos , Lipopolissacarídeos/farmacologia , Fenilalanina , Extratos Vegetais/uso terapêutico , Piridoxina , Fator de Necrose Tumoral alfa/metabolismo
5.
Food Res Int ; 155: 111082, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-35400458

RESUMO

Among several extracts from species from Guinea-Bissauan flora, the hydroethanol extract obtained from the leaves of gingerbread plum (Neocarya macrophylla (Sabine) Prance ex F. White.) revealed to be one of the most cytotoxic towards human gastric AGS carcinoma cells. Considering the increasing use of N. macrophylla in the food industry and the abundant biomass of agricultural wastes being generated, the identification of phenolic bioactives has been attained by HPLC-DAD-ESI/MSn and UHPLC-ESI/QTOF/MSn. Twenty-seven phenolic constituents were identified for the first time in the monotypic genus Neosartorya, 5-O-caffeoylquinic acid being detected as the major constituent (4.90 ± 0.20 mg g-1 dry extract). While 15 flavan-3-ols derivatives were determined, the extract is predominantly characterized by the occurrence of quercetin, kaempferol, apigenin and chrysoeriol glycosides. Typical apoptotic changes in gastric adenocarcinoma AGS cells upon exposure to N. macrophylla leaf extract were observed. The apoptotic cell death is mediated by the activation of the mitochondrial pathway, as loss of mitochondrial membrane potential was detected, as well as increased caspase-9 and -3 activities. The industrial relevance of this plant material, along with the data presented here on the potential anticancer effects of N. macrophylla and the efficient extraction of phenolic bioactives using water and ethanol (GRAS substance), calls for further research on the leaves as a potential functional food and/or ingredient.


Assuntos
Carcinoma , Chrysobalanaceae , Cromatografia Líquida de Alta Pressão , Humanos , Fenóis/análise , Fenóis/farmacologia , Extratos Vegetais/farmacologia , Polifenóis/farmacologia , Espectrometria de Massas por Ionização por Electrospray
6.
Molecules ; 27(4)2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-35209101

RESUMO

Two new ursane-type triterpenoids, named Polyanside A (1) and B (2), along with eleven known compounds (3-13), were isolated and elucidated from Maranthes polyandra (Benth.) Prance. The structures of these compounds were elucidated based on chemical evidence and multiple spectroscopic data. Isolated compounds were evaluated for anti-cancer, anti-inflammatory activities, and cytotoxicity on a normal human cell line (BJ). None of them showed activity and cytotoxicity. The hexane fraction was analyzed by GC-MS, resulting in the identification of forty-one compounds. This is the first comprehensive study on the phytochemistry of M. polyandra.


Assuntos
Chrysobalanaceae/química , Compostos Fitoquímicos/análise , Compostos Fitoquímicos/química , Fracionamento Químico , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Estrutura Molecular , Compostos Fitoquímicos/isolamento & purificação , Extratos Vegetais/análise , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação
7.
Gigascience ; 122022 12 28.
Artigo em Inglês | MEDLINE | ID: mdl-37470496

RESUMO

Vigna reflexo-pilosa (créole bean) is a wild legume belonging to the subgenus Ceratoropis and is widely distributed in Asia. Créole bean is the only tetraploid species in the genus Vigna, and it has been shown to derive from the hybridization of Vigna hirtella and Vigna trinervia. In this study, we combined the long-read PacBio technology with the chromatin contact mapping (Hi-C) technique to obtain a chromosome-level assembly of V. reflexo-pilosa. The final assembly contained 998,724,903 bases with an N50 length of 42,545,650 bases. Our gene prediction recovered 99.4% of the highly conserved orthologs based on the BUSCO analysis. To investigate homoeolog expression bias and expression level dominance in the tetraploid, we also sequenced and assembled the genomes of its progenitors. Overall, the majority of the homoeolog pairs (72.9%) displayed no expression bias, and among those that exhibited biased expression, 16.3% showed unbalanced homoeolog expression bias toward the V. trinervia subgenome. Moreover, 41.2% and 36.2% of the expressed gene pairs exhibited transgressive expression and expression level dominance, respectively. Interestingly, the genome-wide expression level dominance in the tetraploid was biased toward the V. trinervia subgenome. The analysis of methylation patterns also revealed that the average methylation levels in coding regions were higher in the V. hirtella subgenome than those in the V. trinervia subgenome. The genomic/transcriptomic resources for these three species are useful not only for the development of elite cultivars in Vigna breeding programs but also to researchers studying comparative genomics and investigating genomic/epigenomic changes following polyploid events.


Assuntos
Chrysobalanaceae , Fabaceae , Vigna , Vigna/genética , Chrysobalanaceae/genética , Tetraploidia , Melhoramento Vegetal , Fabaceae/genética , Genoma de Planta
8.
J Med Food ; 25(1): 97-109, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34714151

RESUMO

This study was conducted to evaluate the phenolic composition, toxicity, and antimicrobial activity of Licania rigida Benth, an underexploited wild Licania species. L. rigida leaf fractions (ethyl alcohol and ethyl acetate) were analyzed for their phenolic compound and flavonoid total, and high-performance liquid chromatography/ultraviolet spectra chromatographic profiles. Regarding the extract biological effects, toxicity was measured by acute oral toxicity in Wistar rats, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] method, and apoptosis indicators with DAPI in VERO cells, whereas well-agar diffusion and broth microdilution assays were applied to evaluate the antimicrobial ability. The phytochemical analysis resulted in significant amounts of phenolic compounds and total flavonoids in the extract and fraction, with flavonol-3-O-glycosylates as the main constituent. Regarding the extract and fraction antimicrobial activity, the results showed a significant effect against gram-positive bacteria and fungi, among which Staphylococcus epidermidis and Candida krusei displayed more susceptibility. No toxicity effects were observed in animals. Concerning the cytotoxicity assay, only the highest dose tested exhibited a minimal toxic effect on the analyzed cell lines. These results are relevant considering the increase of multiresistant microorganisms to conventional treatments applied. Therefore, investigating the pharmacological properties of the genus Licania is promising in the search for new sources of antimicrobial compounds.


Assuntos
Anti-Infecciosos , Chrysobalanaceae , Animais , Antibacterianos , Anti-Infecciosos/toxicidade , Antioxidantes , Chlorocebus aethiops , Testes de Sensibilidade Microbiana , Extratos Vegetais/toxicidade , Ratos , Ratos Wistar , Células Vero
9.
J Toxicol Environ Health A ; 85(7): 276-290, 2022 04 03.
Artigo em Inglês | MEDLINE | ID: mdl-34789080

RESUMO

Brazilian plant biodiversity is a rich alternative source of bioactive compounds since plant-derived extracts and/or their secondary metabolites exhibit potential properties to treat several diseases. In this context, Licania rigida Benth (Chrysobalanaceae Family), a large evergreen tree distributed in Brazilian semi-arid regions, deserves attention for its widespread use in popular medicine, although its biological properties are still poorly studied. The aim of this study was to examine (1) acute and sub-chronic oral toxicity at 2000 mg/kg dose; (2) in vitro cytotoxicity at 0.1; 1; 10; 100 or 1000 µg/ml; (3) in vivo mutagenicity at 5, 10 or 20 mg/ml, and (4) potential antioxidant protective effect of L. rigida aqueous leaf extract of (AELr). No marked apparent toxic and genotoxic effects were observed using in vitro and in vivo assays after in vitro treatment of Chinese hamster ovary cell line (CHO-K1) with AELr or in vivo exposure of Wistar rats and Drosophila melanogaster to different extract concentrations. Concerning the antioxidant effect, the extract exhibited a protective effect by decreasing lipid peroxidation as determined by malondialdehyde levels. No significant changes were observed for glutathione (GSH) levels and activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx). Data demonstrate the beneficial potential of AELr to be employed for therapeutic purposes. However, further studies are required to validate the pharmacological application of this plant extract to develop as a phytotherapeutic formulation.


Assuntos
Chrysobalanaceae/química , Extratos Vegetais/farmacologia , Extratos Vegetais/toxicidade , Animais , Brasil , Células CHO , Cricetulus , Drosophila melanogaster , Peroxidação de Lipídeos/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Folhas de Planta/química , Plantas Medicinais/toxicidade , Ratos Wistar
10.
Braz. J. Pharm. Sci. (Online) ; 58: e19558, 2022. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1394032

RESUMO

Abstract Licania macrophylla is a medicinal plant from the Amazon. It is mainly used in the form of a decoction and has been reported to contain several phenolic compounds. However, the effect of seasonality on the phenolic composition and antioxidant potential of this plant has not been well studied, especially in the Amazon region, an area affected by the rainy and less-rainy seasons. Therefore, we evaluated the seasonality of these aromatic compounds and the antioxidant potential of the extracts from L. macrophylla stem bark. We also determined the correlation between the extraction methods used and precipitation levels during each period for 1 year. The total flavonoid and phenolic content, DPPH-scavenging potential, percentage of phosphomolybdenum complex reduction, and iron-reducing power were quantified. The levels of phenolic compounds were the highest in June, whereas those of flavonoids were the highest in September and October; however, these differences were not significant. The extracts from April, November, and June showed the best results for DPPH scavenging, phosphomolybdenum reduction, and iron reduction power, respectively. Significant differences in the phenolic content and DPPH-scavenging activity were observed between the more- and less-rainy seasons. The total phenolic content was positively correlated with FRAP and DPPH, whereas flavonoid levels were negatively correlated.


Assuntos
Plantas Medicinais/efeitos adversos , Estações do Ano , Extratos Vegetais/análise , Chrysobalanaceae/classificação , Compostos Fenólicos , Antioxidantes/análise , Equipamentos de Medição de Riscos
12.
J Asian Nat Prod Res ; 23(8): 789-795, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32614662

RESUMO

A new prenylated coumestan, campylohirtin A (1), along with fifteen phenolic known compounds (2‒16) and four other known compounds (17‒20), was obtained from the 95% ethanol extract of roots of Campylotropis hirtella. Their structures were elucidated based on extensive spectroscopic analysis (1 D and 2 D-NMR, MS, UV and IR). In vitro antimalarial activities of compounds 1-3, 5-14 and 16 were evaluated by ß-hematin formation inhibition assay. Compared with the positive control chloroquine diphosphate, compounds 8, 11 and 16 exhibited strong antimalarial activity with the IC50 values of 69.9, 33.2 and 75.4 µM, respectively. Compounds 1-3, 5-7 and 12 showed moderate antimalarial activities with IC50 values ranging from 134.6 µM to 578.6 µM.[Formula: see text].


Assuntos
Chrysobalanaceae , Fabaceae , Cumarínicos , Estrutura Molecular , Raízes de Plantas
13.
J Integr Med ; 19(1): 13-19, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33097429

RESUMO

The genus Chrysobalanus is one of the classes of medicinal plants used in the treatment and management of several diseases. This study is aimed at providing up-to-date information on the phytochemical composition and pharmacological uses of Chrysobalanus icaco. Current literature on the Chrysobalanus species was obtained by searching electronic databases such as PubMed, Google Scholar and Web of Science. Of the species in this genus, four have been reported in the literature, but only one (C. icaco) has been extensively studied. C. icaco is rich in several minerals, including potassium, magnesium, calcium and sodium. The plant also contains a host of phytochemicals, such as flavonoids, diterpenes and triterpenes, which have been shown to have pharmacological activity. It can be concluded that C. icaco is a good source of phytochemicals that contribute to its therapeutic uses. However, bioassay-guided isolation of its bioactive compounds is necessary for promoting the development of drugs from this medicinal plant.


Assuntos
Chrysobalanaceae , Compostos Fitoquímicos/farmacologia , Plantas Medicinais , Chrysobalanaceae/química , Etnofarmacologia , Fitoterapia , Extratos Vegetais/farmacologia , Plantas Medicinais/química
14.
Brazilian Journal of Health Review ; 4(3): 13848-13859, 2021. graf, ilus
Artigo em Português | MOSAICO - Saúde integrativa | ID: biblio-1283604

RESUMO

O Diabetes mellitus tipo II (DM II) é uma síndrome metabólica com uma das maiores prevalências mundiais. A Organização Mundial da Saúde (OMS, 2019) estima que até 2045 teremos aproximadamente 629 milhões de diabéticos adultos no planeta. Além disso, a Federação Internacional de Diabetes (IDF, 2019) estima que o gasto anual global com tratamentos para diabetes adultos em 2017 foi de U$850 bilhões de dólares. Com números tão expressivos buscam-se tratamentos naturais efetivo, eficaz e baixo custo para tal distúrbio. Inúmeras plantas já estão sendo utilizadas como coadjuvante no tratamento do DM II por possuírem ação antidiabética. Porém, há uma incidência na procura de ampliar o acervo de plantas medicinais com compostos hipoglicemiantes. Portanto, este presente trabalho destaca plantas como Croton cajucara Benth, Licania rígida Benth e Morus nigra L. e seus compostos hipoglicemiantes como alternativa em potencial para tratamento desta síndrome metabólica. Os resultados demonstraram a importância das mesmas para a terapêutica do DM II com estudos que comprovem a ação, garantia da eficácia e segurança em sua utilização.


Diabetes mellitus type II (DM II) is a metabolic syndrome with one of the highest prevalence worldwide. The World Health Organization (WHO, 2019) estimates that by 2045 we will have approximately 629 milion adult diabetics on the planet. In addition, the International Diabetes Federation (IDF, 2019) estimates that the annual global expenditure on treatments for adult diabetes in 2017 was $ 850 bilion dollars. With such expressive numbers, effective, efficient and lowcost natural treatments are sought for such disorder. Numerous plants are already being used as an adjuvant in the treatment of DM II because they have an anti-diabetc action. However, there is an incidence in the search to expand the collection of medicinal plants with hypoglicemic compounds. Therefore, this present work highligths plants such as Croton cajucara Benth, Licania rigida Benth and Morus nigra L., and their hypoglycemic compounds as a potential alternative for the treatment of this metabolic syndrome. The results demonstrated the importance of the same for the treatment of DM II with studies that prove the action, guarantee of the efficacy and safety in its use.


Assuntos
Plantas Medicinais , Diabetes Mellitus , Bases de Dados Bibliográficas , Croton , Síndrome Metabólica , Chrysobalanaceae , Hipoglicemiantes
15.
Nutr Hosp ; 37(4): 763-769, 2020 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-32686442

RESUMO

INTRODUCTION: Objective: the purpose of this study was to investigate the effects of Chrysobalanus icaco on adiposity and its mechanism of action in the gene and protein expression of acetyl-CoA carboxylase (ACC), a key enzyme in lipogenesis. Method: Wistar rats were divided into a regular or control group (CG) and a high-fat diet (HFD) group. HFD was treated with saline or aqueous extract of Chrysobalanus icaco (AECI) for four weeks. Body weight and food intake were assessed. Subcutaneous, retroperitoneal and periepididymal adipose tissue samples were collected and weighed. Adipocytes from periepididymal tissue were isolated and analyzed. The gene and protein expression of ACC in subcutaneous tissue was determined. Results: AECI showed no effect on intake or body weight. However, the weight of the fat pads and the gene and protein expression of ACC were lower, and glucose tolerance was improved. Conclusion: the aqueous extract of Chrysobalanus icaco proved beneficial for the treatment of obesity, preventing fat storage and improving glycemic homeostasis.


INTRODUCCIÓN: Objetivo: el objetivo de este estudio fue investigar los efectos del extracto acuoso de Chrysobalanus icaco (AECI) en la adiposidad y su mecanismo de acción en la expresión génica y proteica de la acetil-CoA-carboxilasa (ACC), una enzima clave para la lipogénesis. Métodos: se usaron ratones macho Wistar que se asignaron a una dieta estándar de control (CG) o a una rica en grasa (HFD). La HFD se trató con solución salina o con extracto acuoso de Chrysobalanus icaco (AECI) durante cuatro semanas. Se evaluaron el peso corporal y el consumo alimentario. Se aislaron y analizaron muestras de tejido adiposo subcutáneo, retroperitoneal y periepididímico. Se determinó la expresión génica y proteica de ACC en el tejido subcutáneo. Resultados: el AECI no mostró ningún efecto sobre la ingesta de alimento y tampoco sobre el peso corporal. Sin embargo, el tratamiento con AECI redujo el peso de los tejidos adiposos y la expresión génica y proteica de ACC, y mejoró también la tolerancia a la glucosa. Conclusión: Chrysobalanus icaco (AECI) resultó ser beneficioso para el tratamiento de la obesidad, previniendo el almacenamiento de grasa y mejorando la homeostasis glucémica.


Assuntos
Adiposidade/efeitos dos fármacos , Chrysobalanaceae , Dieta Hiperlipídica , Extratos Vegetais/farmacologia , Acetil-CoA Carboxilase/biossíntese , Acetil-CoA Carboxilase/genética , Adiposidade/genética , Animais , Peso Corporal , Expressão Gênica , Ratos , Ratos Wistar
16.
Regul Toxicol Pharmacol ; 113: 104643, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32199870

RESUMO

Chrysobalanus icaco L. is a native plant of Brazil used as a food source and traditionally for the treatment of various diseases. The aim of study was performed the phytochemical analysis by UPLC-DAD-ESI-QTOF-MS/MS, and evaluated acute and repeated dose oral toxicities of the C. icaco L. leaf aqueous extract (AECi). The acute toxicity study was performed using a dose of AECi 2000 mg/kg, while the repeated dose toxicity study, the AECi was administered daily at doses of 100, 200 and 400 mg/kg, for 28 days. Behavior and mortality of animals were observed during the test period and body weight, as well water and eating consumption. Hematological, biochemical parameters and histopathological examinations were carried out. Phytochemical analysis of the AECi revealed the presence of flavonoids and tannins. Oral single dose of 2000 mg/kg of AECi resulted in no mortalities or abnormal clinical signs. Studies of repeated dose toxicity promoted a reduction in the body weight of treated animals and an increase of hepatic enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in both, males and females. Histopathological analyzes showed alterations in the livers of animals treated with AECi. Thus, this study recommends the population take care when using this species, especially during prolonged periods.


Assuntos
Peso Corporal/efeitos dos fármacos , Chrysobalanaceae/química , Fígado/efeitos dos fármacos , Compostos Fitoquímicos/toxicidade , Extratos Vegetais/toxicidade , Folhas de Planta/química , Administração Oral , Animais , Feminino , Fígado/patologia , Masculino , Medicina Tradicional , Camundongos , Compostos Fitoquímicos/administração & dosagem , Extratos Vegetais/administração & dosagem , Testes de Toxicidade Aguda , Testes de Toxicidade Subaguda , Água/química
17.
Food Chem ; 313: 126117, 2020 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-31951881

RESUMO

Licania tomentosa is a Brazilian plant species that produces edible fruits, yet there is little information available concerning their nutritional and/or bioactive composition. This study aimed to evaluate the nutritional and polyphenol composition of L. tomentosa fruits (pulp and seeds) and measure antioxidant activity in ethanolic extracts.The pulp and seeds were excellent sources of fiber (25.62%-41.70%) as well as minerals and vitamins. L. tomentosa contained no lectins or protease inhibitors (chymotrysin and trypsin) and 12 polyphenol compounds were identified in the seed extracts with a predominance of flavonoids. The seeds also presented antioxidant activities using the DPPH (SC5010.30-15.87 µg/mL), TBARS (IC50 18.46-20.84 µg/mL), and FRAP (RC50 0.203-0.309 µg/mL) assays. Due to its nutrient and antioxidant content, L. tomentosa may be used for food applications.


Assuntos
Antioxidantes/química , Chrysobalanaceae/química , Valor Nutritivo , Extratos Vegetais/química , Brasil , Cromatografia Líquida de Alta Pressão , Chrysobalanaceae/metabolismo , Flavonoides/química , Farinha/análise , Frutas/química , Frutas/metabolismo , Polifenóis/análise , Polifenóis/química , Inibidores de Proteases/análise , Inibidores de Proteases/química , Sementes/química , Sementes/metabolismo , Espectrometria de Massas por Ionização por Electrospray
18.
Rev. cuba. plantas med ; 25(1): 1-11, 2020. tab, graf
Artigo em Espanhol | MOSAICO - Saúde integrativa | ID: biblio-1223995

RESUMO

Introducción: Chrysobalanus icaco L. (Chrysobalanaceae) es un arbusto utilizado en la fitoterapia tradicional latinoamericana, pero en la provincia de Chiriquí se ha registrado el uso de su fruto para hacer conservas, no de forma medicinal. Objetivo: Caracterizar los metabolitos secundarios presentes en las hojas y semillas de Chrysobalanus icaco y evaluar su actividad biológica in vitro. Métodos: Los metabolitos secundarios presentes en los extractos de las hojas, semilla inmadura y madura de Chrysobalanus icaco, se detectaron a través de tamizaje fitoquímico y purificación por técnicas cromatográficas. Posteriormente se evaluó el contenido de polifenoles totales y la actividad antioxidante total de la infusión de las hojas, semillas y pulpa de esta especie, así como la actividad antibacteriana in vitro. Resultados: En las hojas se identificaron por tamizaje fitoquímico, flavonoides, glucósidos cardiotónicos, triterpenos y esteroides. Para la semilla inmadura se encontró presencia mayoritaria de mezclas de azucares, mientras que para la semilla madura se detectaron ácidos grasos en el endocarpio (almendra). Los datos de polifenoles totales en extractos de Icaco mostraron alto contenido de estos metabolitos en la infusión de hojas secas, y la inhibición del radical DPPH fue mayor también para las hojas secas de icaco, seguido de la infusión de semilla inmadura. Por otro lado, la infusión de hojas secas y el licuado de la pulpa fueron los que mostraron una mayor inhibición contra la cepa de Staphylococcus spp. en el ensayo de actividad antibacteriana. Conclusión: Las hojas secas de Chrysobalanus icaco mostraron alto contenido de polifenoles, asociado a su potencial actividad antioxidante y antibacteriana. Además, se aportaron nuevos datos de composición química de la semilla, se observó que los azúcares detectados inicialmente en la semilla inmadura se pierden considerablemente con la maduración del fruto.


Introduction: Chrysobalanus icaco L. (Chrysobalanaceae) is a shrub used in Latin American traditional phytotherapy, but in the province of Chiriquí its fruit has been reported to be used in food preserves, not for medicinal purposes. Objective: Characterize the secondary metabolites present in Chrysobalanus icaco leaves and seeds and evaluate their in vitro biological activity. Methods: The secondary metabolites present in extracts from leaves and immature and mature seeds of icaco were detected by phytochemical screening and purification with chromatographic techniques. Evaluation was then conducted of total polyphenolic content and total antioxidant activity of the leaf, seed and pulp infusion as well as its antibacterial activity in vitro. Results: Phytochemical screening of the leaves found flavonoids, cardiotonic glycosides, triterpenes and steroids. Sugar mixtures were the most abundant components in immature seeds, whereas fatty acids were found in the endocarp (almond) of mature seeds. Total polyphenolic data about icaco extracts showed high contents of these metabolites in the dry leaf infusion. DPPH radical inhibition was also greater for icaco dry leaves, followed by immature leaf infusion. The dry leaf infusion and the pulp shake displayed the greatest inhibition against the Staphylococcus spp. strain in the antibacterial activity test. Conclusion: Icaco dry leaves exhibited a high polyphenolic content, associated to their antioxidant and antibacterial activity. Fresh data were also contributed about the chemical composition of the seed. It was observed that the sugars initially detected in immature seeds are considerably lost as the fruit ripens.


Assuntos
Chrysobalanaceae , Compostos Fitoquímicos , Antibacterianos , Antioxidantes , Técnicas In Vitro , Medicina Tradicional
19.
Brazilian Journal of Development ; 6(11): 86190-86202, 2020. tab, ilus
Artigo em Inglês | MOSAICO - Saúde integrativa | ID: biblio-1147604

RESUMO

Chrysobalanus icaco L. (Chrysobalanaceae) is a medicinal species widely used in Brazil mainly to treat diabetes. Despite the medicinal importance of C. icaco, genetic information of this genus remains limited. Thus, our aim was to evaluate the influence of the genetic basis of C. icaco by determining its chemotypes. 25 C. icaco genotypes were collected from 15 sites in Belém, Marajó and Northeastern mesoregions of Pará state, Brazil. The genotypes were selected by evaluating the plant morphological characteristics such as fruit color and plant habit. The DNA fingerprinting profile was performed using PCR based RAPD technique and appropriate statistical methods were used. RAPD markers were used for evaluation of genetic diversity and molecular characterization of the C. icaco, using a total of 18 decamer primers. These primers produced 85 amplification products, with an average of 4.7 bands per primer and 99.2% polymorphism. The genotypes are genetically distinct, forming variable clusters in number and constitution by different methods. By the morphological characteristics considered, there is a tendency of clustering based on the color of the ripe fruit. We found the secondary metabolite content depends not on environmental condition, but rather on C. icaco genome. Therefore, it may have implications for ethnopharmacological use of the chemotypes.


Assuntos
Humanos , Chrysobalanaceae , Plantas Medicinais , Brasil , Impressões Digitais de DNA , Etnofarmacologia , Diabetes Mellitus
20.
Int J Biol Macromol ; 140: 294-302, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31422188

RESUMO

Several plants have been studied for their medicinal properties, especially concerning the management of chronic diseases, such as diabetes, aiming at a more accessible form of treatment. In this context, the aim of this study was to characterize plant proteins used in folk medicine as hypoglycemic agents for the treatment of diabetes, namely "abajerú" (Chrysobalanus icaco) and "cow's paw" (Bauhinia forficata and Bauhinia variegata). The species were differentiated by proteome characterization. Proteins were in-solution digested using trypsin by the filter-assisted sample preparation (FASP) method. Peptides were then analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS) for protein characterization. In total, 131 proteins were identified. The main biological functions of these proteins were cellular respiration, transport, metabolism and photosynthesis. Insulin-like proteins were not detected, but proteins involved in controlling glucose levels were. The results are of value in the proteomic characterization of phytotherapeutic plants, and may serve as baseline for the assessed species in Brazil, where a lack of knowledge in this regard is observed.


Assuntos
Bauhinia/metabolismo , Chrysobalanaceae/metabolismo , Diabetes Mellitus/tratamento farmacológico , Hipoglicemiantes/farmacologia , Extratos Vegetais/farmacologia , Proteoma , Brasil , Cromatografia Líquida , Geografia , Hipoglicemiantes/química , Medicina Tradicional , Extratos Vegetais/química , Folhas de Planta/metabolismo , Plantas Medicinais , Proteômica , Espectrometria de Massas em Tandem
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