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1.
Methods Mol Biol ; 2552: 409-433, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36346606

RESUMO

In the computational design of antibodies, the interaction analysis between target antigen and antibody is an essential process to obtain feedback for validation and optimization of the design. Kinetic and thermodynamic parameters as well as binding affinity (KD) allow for a more detailed evaluation and understanding of the molecular recognition. In this chapter, we summarize the conventional experimental methods which can calculate KD value (ELISA, FP), analyze a binding activity to actual cells (FCM), and evaluate the kinetic and thermodynamic parameters (ITC, SPR, BLI), including high-throughput analysis and a recently developed experimental technique.


Assuntos
Anticorpos , Antígenos , Ensaio de Imunoadsorção Enzimática , Cinética , Computadores
3.
Appl Ergon ; 106: 103896, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36156386

RESUMO

Worker guidance systems provide product-specific and digital assembly information and can make an important contribution to increasing productivity and quality and relieving employees, especially in flexible variant assembly. However, a critical factor here is the successful design of the systems. While much of the research focuses on aspects of the hardware, this article is dedicated to the question of how the configuration of the level of information, i.e. the amount and depth of information in worker guidance systems, is affected. For this purpose, two studies, a laboratory study at the Technical University of Darmstadt (N = 53) and a field study at a company (N = 30), were conducted and two variants of the information level of a worker guidance system were compared. The study results show that assembly quality in particular is significantly influenced by the level of information; to the advantage of detailed information. No significant differences were found for production times. For the subjective evaluation of the system as well as for the objective and subjectively perceived strain, it can be stated that the high level of information also performs better. The results thus help to design worker guidance systems more precisely so that they fulfill the information needs of the employees as well as possible.


Assuntos
Computadores , Eficiência , Humanos
4.
Radiol Clin North Am ; 61(1): 141-150, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36336387

RESUMO

Peripheral vascular injuries are a rare finding in the setting of trauma but an important source of morbidity and mortality when present. Fast and accurate diagnosis followed by rapid repair of vascular injuries are important for achieving the best clinical outcomes. The advancements in computer tomography (CT) and decades of experience in vascular imaging have allowed radiologists to become important contributors for the diagnosis and characterization of peripheral vascular injury. We review the epidemiology of peripheral vascular injuries, indications for imaging, ways to optimize CT technique, imaging findings, and common challenges for accurate diagnosis of such injuries.


Assuntos
Lesões do Sistema Vascular , Humanos , Lesões do Sistema Vascular/diagnóstico por imagem , Angiografia por Tomografia Computadorizada , Tomografia Computadorizada por Raios X/métodos , Angiografia/métodos , Computadores
5.
Mol Phylogenet Evol ; 178: 107636, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36208695

RESUMO

Phylogenetic trees are essential tools in evolutionary biology that present information on evolutionary events among organisms and molecules. From a dataset of n sequences, a phylogenetic tree of (2n-5)!! possible topologies exists, and determining the optimum topology using brute force is infeasible. Recently, a recursive graph cut on a graph-represented-similarity matrix has proven accurate in reconstructing a phylogenetic tree containing distantly related sequences. However, identifying the optimum graph cut is challenging, and approximate solutions are currently utilized. Here, a phylogenetic tree was reconstructed with an improved graph cut using a quantum-inspired computer, the Fujitsu Digital Annealer (DA), and the algorithm was named the "Normalized-Minimum cut by Digital Annealer (NMcutDA) method". First, a criterion for the graph cut, the normalized cut value, was compared with existing clustering methods. Based on the cut, we verified that the simulated phylogenetic tree could be reconstructed with the highest accuracy when sequences were diverged. Moreover, for some actual data from the structure-based protein classification database, only NMcutDA could cluster sequences into correct superfamilies. Conclusively, NMcutDA reconstructed better phylogenetic trees than those using other methods by optimizing the graph cut. We anticipate that when the diversity of sequences is sufficiently high, NMcutDA can be utilized with high efficiency.


Assuntos
Algoritmos , Computadores , Filogenia , Análise por Conglomerados , Bases de Dados de Proteínas
6.
Sci Total Environ ; 856(Pt 1): 159096, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36181826

RESUMO

Antibiotic resistance genes (ARGs) have been regarded as emerging pollutants due to their potential risk of resistance. Horizontal gene transfer (HGT) is the main pathway for ARGs to lead to environmental threats. Therefore, the inhabitation of ARGs' HGT can effectively inhibit ARGs' potential drug resistance risk within a single strain. In this paper, the characteristics of ARGs' HGT in paddy and dryland soils were identified and regulated by a combination of ARGs' HGT feature identification, transfer mechanism analysis and transfer process regulation. The homology modeling algorithm was used to simulate the construction of the Tn5 plasmid transposase of Escherichia coli (E. coli) for identifying ARGs' HGT characteristics. The GCG (212.617 Å) was thus determined as the target codon. Through integrated computer-based methods, results showed that the most important environmental disturbance factors for the HGT of ARGs in the paddy and dryland soils were rough farmyard manure/sewage irrigation and mining pollution, respectively. Under the disturbance of key environmental factors, the inhibitory effect of HGT of ARGs in paddy and dryland soil was reduced by 35.01 % and 34.74 %, respectively. Results demonstrated that the proposed theoretical mechanism and control strategies could effectively inhibit the HGT of E. coli ARGs in the soil environment.


Assuntos
Transferência Genética Horizontal , Solo , Antibacterianos/farmacologia , Escherichia coli/genética , Genes Bacterianos , Resistência Microbiana a Medicamentos/genética , Computadores
7.
J. Health Biol. Sci. (Online) ; 10(1): 1-5, 01/jan./2022. ilus
Artigo em Inglês | LILACS | ID: biblio-1370898

RESUMO

Introduction: The use of computers, tablets, and cell phones with the Internet by people with quadriplegia from spinal cord injuries is much lower when compared to the general population. The quality of life of these people can be substantially improved through access to these technologies, which would allow a quadriplegic to take advantage of the rapid evolution of information and communication. Many of these individuals have the function of preserved neck and mouth muscles, and it is possible to manipulate intraoral devices as an adaptation tool. Case Report: We report an intraoral device installation in a patient with quadriplegia, a victim of a firearm accident, who had a great desire to reuse the tablet for internet access. A device made of acrylic resin, similar to a myorelaxant plate, was designed to allow the use of a computer that was comfortable and at the same time, the patient could talk and not harm the dental structures. The person responsible agreed to participate in the research and signed the consent form. In addition, the work was submitted to an ethics committee. Considerations: The ideal intra-oral device for patient rehabilitation should be inexpensive, easy to adapt and promote muscle relaxation besides allowing the patient to expand their abilities and digitally increase their autonomy for society.


Introdução: O uso de computadores, tablets e celulares com internet por pessoas com tetraplegia por lesão medular é muito menor quando comparado à população geral. A qualidade de vida dessas pessoas pode ser, substancialmente, melhorada por meio do acesso a essas tecnologias, o que permitiria aos tetraplégicos aproveitar a rápida evolução da informação e da comunicação. Muitos desses indivíduos têm a função de músculos cervicais e bucais preservados, sendo possível a manipulação de dispositivos intraorais como ferramenta de adaptação. Relato de Caso: Relatamos a instalação de um dispositivo intraoral em um paciente com tetraplegia, vítima de acidente com arma de fogo, que tinha grande desejo de reutilizar o tablet para acesso à internet. Um dispositivo feito de resina acrílica, semelhante a uma placa miorrelaxante, foi projetado para permitir o uso de um computador que fosse confortável e, ao mesmo tempo, o paciente pudesse falar e não prejudicar as estruturas dentárias. O responsável concordou em participar da pesquisa e assinou o termo de consentimento. Além disso, o trabalho foi submetido a um comitê de ética. Considerações: O dispositivo intraoral ideal para a reabilitação do paciente deve ser barato, de fácil adaptação e promover o relaxamento muscular, além de permitir que o paciente amplie suas habilidades e aumente sua autonomia pessoal, digitalmente para a sociedade.


Assuntos
Inclusão Digital , Inclusão Social , Quadriplegia , Computadores , Pessoas com Deficiência , Comunicação , Adaptação a Desastres , Autonomia Pessoal
8.
Stud Health Technol Inform ; 299: 104-117, 2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36325851

RESUMO

From beginning to today, pHealth has been a data driven service that collects and uses personal health information (PHI) for personal health services and personalized healthcare. As a result, pHealth services use intensively ICT technology, sensors, computers and mathematical algorithms. In past, pHealth applications were focused to certain health or sickness related problem, but in today they use mobile devices, wireless networks, Web-technology and Cloud platforms. In future, pHealth uses information systems that are highly distributed, dynamic, increasingly autonomous, multi-stakeholder data driven eco-system having ability to monitor anywhere person's regular life, movements and health related behaviours. Because privacy and trust are pre-requirements for successful pHealth, this development raises huge privacy and trust challenges to be solved. Researchers have shown that current privacy approaches and solutions used in pHealth do not offer acceptable level of privacy, and trust is only an illusion. This indicates, that today's privacy models and technology shall not be moved to the future pHealth. The authors have analysed interesting new privacy and trust ideas published in journals, and found that they seem to be effective but offer only a partial solution. To solve this weakness, the authors used a holistic system view to aspects impacting privacy and trust in pHealth, and created a template that can be used in planning and development future pHealth services. The authors also propose a tentative solution for future trustworthy pHealth. It combines privacy as personal property and trust as legal binding fiducial duty approaches, and uses a Blockchain-based smart contract solution to store person's privacy and trust requirements and service providers' promises.


Assuntos
Registros de Saúde Pessoal , Privacidade , Humanos , Confiança , Computadores , Computadores de Mão
9.
BMC Ophthalmol ; 22(1): 430, 2022 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-36368980

RESUMO

BACKGROUND: To assess whether informing patients with a computer-based tutorial in addition to standard informed consent influences the patient's attitude towards surgery and increases patient's knowledge. METHODS: In this prospective, exploratory, randomized clinical study, patients scheduled for their first eye cataract surgery were randomly allocated to two groups, receiving standard face-to-face informed consent (control group) or additionally using an interactive computer-based tool (CatInfo) containing an audiovisual presentation about cataract and its treatment (study group). Cataract-related knowledge and decisional confidence (decisional conflict scale (DCS)) were assessed as well as one-month postoperatively decisional regret (decision regret scale (DRS)) and willingness to exchange face-to-face discussion time for the use of such a tool. RESULTS: The study comprised 134 patients, 64 patients in the study group and 70 in the control group. Patients in the study group answered more questions correctly, 16.3 ± 2.0 (median 16.5, 11.0-19.0) versus 15.5 ± 1.9 (median 16.0, 8.0-19.0; p = 0.01). Patients showed a high decisional confidence with a study group mean DCS score of 92.4 ± 9.8 (median 96.9, 65.6-100) and control group score of 91.6 ± 10.9 (median 95.3, 43.3-100; p = 0.52). Mean DRS score in the study group was 2.5 ± 8.0 (median 0, 0-40) and 4.3 ± 12.5 (median 0, 0-75) in the control group (p = 0.14). Of study group patients 23 (67.6%) were willing to trade time, on average 158 ± 180 s (median 120 s, 45-900). Satisfaction with the tool was high with a mean of 9.1 ± 1.3 out of 10 (median 9.7, 5.0-10). CONCLUSIONS: Cataract-related knowledge was generally good, with slightly higher scores in the study group. In both groups, decisional confidence was high and regret after surgery was low. A tendency towards slightly higher decisional confidence and lower regret was found in the study group, although these differences were not statistically significant. Additional use of an interactive computer-based tool may prove useful in the informed consent process in a high-volume cataract outpatient setting. TRIAL REGISTRATION: ClinicalTrials.gov, NCT04975126. Retrospectively registered - July 23, 2021.


Assuntos
Extração de Catarata , Catarata , Humanos , Estudos Prospectivos , Consentimento Livre e Esclarecido , Computadores
10.
Comput Intell Neurosci ; 2022: 8342638, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36407688

RESUMO

Federated learning (FL), a distributed machine-learning framework, is poised to effectively protect data privacy and security, and it also has been widely applied in variety of fields in recent years. However, the system heterogeneity and statistical heterogeneity of FL pose serious obstacles to the global model's quality. This study investigates server and client resource allocation in the context of FL system resource efficiency and offers the FedAwo optimization algorithm. This approach combines adaptive learning with federated learning, and makes full use of the computing resources of the server to calculate the optimal weight value corresponding to each client. This approach aggregated the global model according to the optimal weight value, which significantly minimizes the detrimental effects of statistical and system heterogeneity. In the process of traditional FL, we found that a large number of client trainings converge earlier than the specified epoch. However, according to the provisions of traditional FL, the client still needs to be trained for the specified epoch, which leads to the meaningless of a large number of calculations in the client. To further lower the training cost, the augmentation FedAwo ∗ algorithm is proposed. The FedAwo ∗ algorithm takes into account the heterogeneity of clients and sets the criteria for local convergence. When the local model of the client reaches the criteria, it will be returned to the server immediately. In this way, the epoch of the client can dynamically be modified adaptively. A large number of experiments based on MNIST and Fashion-MNIST public datasets reveal that the global model converges faster and has higher accuracy in FedAwo and FedAwo ∗ algorithms than FedAvg, FedProx, and FedAdp baseline algorithms.


Assuntos
Algoritmos , Aprendizado de Máquina , Humanos , Computadores
11.
Turk J Ophthalmol ; 52(5): 295-301, 2022 10 28.
Artigo em Inglês | MEDLINE | ID: mdl-36317741

RESUMO

Objectives: The aim of this study was to investigate the prevalence of symptoms of computer vision syndrome (CVS) and identify its associated risk factors among computer-using bank workers in Pakistan. Materials and Methods: This cross-sectional study was conducted on computer-using bank workers. The data collection procedure included a self-administered questionnaire and comprehensive ocular health examination. The prevalence of CVS and its associated risk factors were investigated. The chi-square test was used to study the significance of the association of CVS with potential risk factors. Results: Of 127 participants, 95 (74.8%) were men. Most of the participants (n=53; 41.7%) were in the 30-40 years age group. A total of 101 participants (79.5%) reported any ocular symptom, with burning eyes being the most frequent ocular symptom (77.2%). General body fatigue was the most common non-ocular symptom of CVS (92.9%), followed by headache (83.5%). Out of 127 participants, 71 men and 30 women had some degree of CVS. Female participants had significantly higher risk of CVS than male participants (p=0.01). Total duration of computer use per day and duration of uninterrupted computer use were significantly associated with the occurrence of CVS (p=0.001 and p=0.008, respectively). No significant association was found between CVS and distance from computer screen (p=0.89), frequency of breaks (p=0.18), or font size (p=0.12). Conclusion: A high prevalence of CVS-related symptoms was observed among computer-using bank workers. Non-ocular symptoms associated with computer use were more common than ocular symptoms (92.9% vs. 77.2%).


Assuntos
Computadores , Feminino , Masculino , Humanos , Prevalência , Estudos Transversais , Paquistão/epidemiologia , Síndrome , Fatores de Risco
12.
Nat Commun ; 13(1): 7018, 2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36384960

RESUMO

Neuromorphic machines are intriguing for building energy-efficient intelligent systems, where spiking neurons are pivotal components. Recently, memristive neurons with promising bio-plausibility have been developed, but with limited reliability, bulky capacitors or additional reset circuits. Here, we propose an anti-ferroelectric field-effect transistor neuron based on the inherent polarization and depolarization of Hf0.2Zr0.8O2 anti-ferroelectric film to meet these challenges. The intrinsic accumulated polarization/spontaneous depolarization of Hf0.2Zr0.8O2 films implements the integration/leaky behavior of neurons, avoiding external capacitors and reset circuits. Moreover, the anti-ferroelectric neuron exhibits low energy consumption (37 fJ/spike), high endurance (>1012), high uniformity and high stability. We further construct a two-layer fully ferroelectric spiking neural networks that combines anti-ferroelectric neurons and ferroelectric synapses, achieving 96.8% recognition accuracy on the Modified National Institute of Standards and Technology dataset. This work opens the way to emulate neurons with anti-ferroelectric materials and provides a promising approach to building high-efficient neuromorphic hardware.


Assuntos
Redes Neurais de Computação , Neurônios , Reprodutibilidade dos Testes , Neurônios/fisiologia , Sinapses/fisiologia , Computadores
13.
Nat Commun ; 13(1): 7019, 2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36384983

RESUMO

Selective attention is an efficient processing strategy to allocate computational resources for pivotal optical information. However, the hardware implementation of selective visual attention in conventional intelligent system is usually bulky and complex along with high computational cost. Here, programmable ferroelectric bionic vision hardware to emulate the selective attention is proposed. The tunneling effect of photogenerated carriers are controlled by dynamic variation of energy barrier, enabling the modulation of memory strength from 9.1% to 47.1% without peripheral storage unit. The molecular polarization of ferroelectric P(VDF-TrFE) layer enables a single device not only multiple nonvolatile states but also the implementation of selective attention. With these ferroelectric devices are arrayed together, UV light information can be selectively recorded and suppressed the with high current decibel level. Furthermore, the device with positive polarization exhibits high wavelength dependence in the image attention processing, and the fabricated ferroelectric sensory network exhibits high accuracy of 95.7% in the pattern classification for multi-wavelength images. This study can enrich the neuromorphic functions of bioinspired sensing devices and pave the way for profound implications of future bioinspired optoelectronics.


Assuntos
Biônica , Visão Ocular , Computadores
15.
Prog Orthod ; 23(1): 39, 2022 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-36404385

RESUMO

BACKGROUND: With advances in digital technology, new types of lingual fixed retainers are being developed. However, there are few studies that quantitatively evaluate the accuracy and stability of lingual fixed retainers. The aim of this study was to assess the accuracy and stability of two types of computer-aided customized lingual fixed retainers and a conventional lingual fixed retainer. METHODS: A total of 10 maxillary and 10 mandibular duplicated dental models were selected, and then, three types of retainers were fabricated on the canine-to-canine area for each model. To evaluate accuracy, wire clearance at interproximal area (WCI) was measured using superimposition analysis. Initial flatness deformation was also measured for vertical distortion of retainers. Lateral width, anteroposterior length, and flatness deformation were measured at three-time points for stability assessment. Thermocycling was used to induce 6 months of time flow. RESULTS: The custom-bent group showed significantly higher WCI than the custom-cut and manual groups in the maxillary arch (P = 0.002). The custom-cut group showed significantly less flatness deformation, which was followed by the custom-bent and manual groups in both the maxillary and mandibular arch (P < 0.001). There was no significant difference in stability between the three retainer groups during 5100 cycles of thermocycling (corresponding to 6-month period). CONCLUSIONS: Since there was no difference in stability between the three groups, it is recommended to use custom-cut type retainers in light of accuracy. However, accuracy and stability are not the only factors to consider when selecting type of retainers. Because each retainer has advantages and disadvantages, the type of retainers should be decided in consideration of the clinical environment.


Assuntos
Aparelhos Ortodônticos Fixos , Contenções Ortodônticas , Projetos Piloto , Maxila , Computadores
16.
Artigo em Inglês | MEDLINE | ID: mdl-36360992

RESUMO

Nowadays, water pollution has become a global issue affecting most countries in the world. Water quality should be monitored to alert authorities on water pollution, so that action can be taken quickly. The objective of the review is to study various conventional and modern methods of monitoring water quality to identify the strengths and weaknesses of the methods. The methods include the Internet of Things (IoT), virtual sensing, cyber-physical system (CPS), and optical techniques. In this review, water quality monitoring systems and process control in several countries, such as New Zealand, China, Serbia, Bangladesh, Malaysia, and India, are discussed. Conventional and modern methods are compared in terms of parameters, complexity, and reliability. Recent methods of water quality monitoring techniques are also reviewed to study any loopholes in modern methods. We found that CPS is suitable for monitoring water quality due to a good combination of physical and computational algorithms. Its embedded sensors, processors, and actuators can be designed to detect and interact with environments. We believe that conventional methods are costly and complex, whereas modern methods are also expensive but simpler with real-time detection. Traditional approaches are more time-consuming and expensive due to the high maintenance of laboratory facilities, involve chemical materials, and are inefficient for on-site monitoring applications. Apart from that, previous monitoring methods have issues in achieving a reliable measurement of water quality parameters in real time. There are still limitations in instruments for detecting pollutants and producing valuable information on water quality. Thus, the review is important in order to compare previous methods and to improve current water quality assessments in terms of reliability and cost-effectiveness.


Assuntos
Internet das Coisas , Qualidade da Água , Reprodutibilidade dos Testes , Poluição da Água , Computadores
17.
Artigo em Inglês | MEDLINE | ID: mdl-36361190

RESUMO

Patients with mild traumatic brain injury (MTBI) with intracerebral hemorrhage (ICH), particularly those at higher risk of having ICH progression, are typically prescribed a second head Computer Tomography (CT) scan to monitor the disease development. This study aimed to evaluate the role of a repeat head CT in MTBI patients at a higher risk of ICH progression by comparing the intervention rate between patients with and without ICH progression. METHODS: 192 patients with MTBI and ICH were treated between November 2019 to December 2020 at a single level II trauma center. The Glasgow Coma Scale (GCS) was used to classify MTBI, and initial head CT was performed according to the Canadian CT head rule. Patients with a higher risk of ICH progression, including the elderly (≥65 years old), patients on antiplatelets or anticoagulants, or patients with an initial head CT that revealed EDH, contusional bleeding, or SDH > 5 mm, and multiple ICH underwent a repeat head CT within 12 to 24 h later. Data regarding types of intervention, length of stay in the hospital, and outcome were collected. The risk of further neurological deterioration and readmission rates were compared between these two groups. All patients were followed up in the clinic after one month or contacted via phone if they did not return. RESULTS: 189 patients underwent scheduled repeated head CT, 18% had radiological intracranial bleed progression, and 82% had no changes. There were no statistically significant differences in terms of intervention rate, risk of neurological deterioration in the future, or readmission between them. CONCLUSION: Repeat head CT in mild TBI patients with no neurological deterioration is not recommended, even in patients with a higher risk of ICH progression.


Assuntos
Concussão Encefálica , Humanos , Idoso , Canadá , Tomografia Computadorizada por Raios X/métodos , Hemorragia Cerebral/diagnóstico por imagem , Computadores , Encéfalo , Estudos Retrospectivos
18.
Artigo em Inglês | MEDLINE | ID: mdl-36361209

RESUMO

Deep learning for the analysis of H&E stains requires a large annotated training set. This may form a labor-intensive task involving highly skilled pathologists. We aimed to optimize and evaluate computer-assisted annotation based on digital dual stains of the same tissue section. H&E stains of primary and metastatic melanoma (N = 77) were digitized, re-stained with SOX10, and re-scanned. Because images were aligned, annotations of SOX10 image analysis were directly transferred to H&E stains of the training set. Based on 1,221,367 annotated nuclei, a convolutional neural network for calculating tumor burden (CNNTB) was developed. For primary melanomas, precision of annotation was 100% (95%CI, 99% to 100%) for tumor cells and 99% (95%CI, 98% to 100%) for normal cells. Due to low or missing tumor-cell SOX10 positivity, precision for normal cells was markedly reduced in lymph-node and organ metastases compared with primary melanomas (p < 0.001). Compared with stereological counts within skin lesions, mean difference in tumor burden was 6% (95%CI, -1% to 13%, p = 0.10) for CNNTB and 16% (95%CI, 4% to 28%, p = 0.02) for pathologists. Conclusively, the technique produced a large annotated H&E training set with high quality within a reasonable timeframe for primary melanomas and subcutaneous metastases. For these lesion types, the training set generated a high-performing CNNTB, which was superior to the routine assessments of pathologists.


Assuntos
Melanoma , Neoplasias Cutâneas , Humanos , Carga Tumoral , Redes Neurais de Computação , Computadores , Fatores de Transcrição SOXE
19.
Int J Mol Sci ; 23(21)2022 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-36361573

RESUMO

This review of our experience in computer-assisted tissue image analysis (CATIA) research shows that significant information can be extracted and used to diagnose and distinguish normal from abnormal endometrium. CATIA enabled the evaluation and differentiation between the benign and malignant endometrium during diagnostic hysteroscopy. The efficacy of texture analysis in the endometrium image during hysteroscopy was examined in 40 women, where 209 normal and 209 abnormal regions of interest (ROIs) were extracted. There was a significant difference between normal and abnormal endometrium for the statistical features (SF) features mean, variance, median, energy and entropy; for the spatial grey-level difference matrix (SGLDM) features contrast, correlation, variance, homogeneity and entropy; and for the gray-level difference statistics (GLDS) features homogeneity, contrast, energy, entropy and mean. We further evaluated 52 hysteroscopic images of 258 normal and 258 abnormal endometrium ROIs, and tissue diagnosis was verified by histopathology after biopsy. The YCrCb color system with SF, SGLDM and GLDS color texture features based on support vector machine (SVM) modeling correctly classified 81% of the cases with a sensitivity and a specificity of 78% and 81%, respectively, for normal and hyperplastic endometrium. New technical and computational advances may improve optical biopsy accuracy and assist in the precision of lesion excision during hysteroscopy. The exchange of knowledge, collaboration, identification of tasks and CATIA method selection strategy will further improve computer-aided diagnosis implementation in the daily practice of hysteroscopy.


Assuntos
Diagnóstico por Computador , Histeroscopia , Gravidez , Humanos , Feminino , Histeroscopia/métodos , Endométrio/diagnóstico por imagem , Endométrio/patologia , Biópsia , Computadores , Sensibilidade e Especificidade
20.
Int J Mol Sci ; 23(21)2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36362252

RESUMO

Food-derived tripeptides can relieve colitis symptoms; however, their alleviation mode has not been systematically evaluated as an alternative nutritional compound. This study aimed to reveal the potential mechanism of 8000 food-derived tripeptides against acute colitis using a computer-aided screening strategy. Forty-one potential hub targets related to colitis with a Fit score > 4.0 were screened to construct the protein-protein and protein-tripeptide network based on the PharmMapper database and STRING software (Ver. 11.5). In addition, 30 significant KEGG signaling pathways with p-values < 0.001 that the 41 hub targets mainly participated in were identified using DAVID software (Ver. 6.8), including inflammatory, immunomodulatory, and cell proliferation and differentiation-related signaling pathways, particularly in the Ras- and PI3K-Akt signaling pathways. Furthermore, molecular docking was performed using the Autodock against majorly targeted proteins (AKT1, EGFR, and MMP9) with the selected 52 tripeptides. The interaction model between tripeptides and targets was mainly hydrogen-bonding and hydrophobic interactions, and most of the binding energy of the tripeptide target was less than -7.13 kcal/mol. This work can provide valuable insight for exploring food-derived tripeptide mechanisms and therapeutic indications.


Assuntos
Colite , Medicamentos de Ervas Chinesas , Humanos , Simulação de Acoplamento Molecular , Fosfatidilinositol 3-Quinases , Medicamentos de Ervas Chinesas/química , Colite/tratamento farmacológico , Computadores
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