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1.
J Vis Exp ; (173)2021 07 31.
Artigo em Inglês | MEDLINE | ID: mdl-34398138

RESUMO

Several studies have demonstrated that the phytochemical contents of plants are potential anti-obesity agents. In this study we examine the effect of using a combination of dry buttons from Syzygium aromaticum and seeds from Cuminum cyminum (CC) on C57BL6/J mice induced with obesity via high-fat-diet (HFD). The aim of this study is to demonstrate that the method proposed in the study reduced obesity significantly after several weeks of experimentation. The extract from both plants was extracted using ultrasound to enhance the extraction of phytochemicals. Optimum extraction conditions were obtained with ethanol as follows: 50:50 v/v water with an ultrasound power of 300 W, and ultrasonication time of 30 minutes. The simultaneous administration of the CC extract in HFD for 5 weeks led to the regulation of lipid profiles (cholesterol and triglycerides), reduction of food intake, weight gain, adipose tissue and liver weight. Findings obtained by this obese model indicate that CC extract can prevent obesity. Compared with the traditional 16 weeks method (8 weeks to get fat, and 8 weeks to lose weight), similar results were obtained in the present study obese model in less time of experimentation.


Assuntos
Cuminum , Syzygium , Tecido Adiposo , Animais , Dieta Hiperlipídica/efeitos adversos , Fígado , Camundongos , Camundongos Endogâmicos C57BL , Obesidade/tratamento farmacológico , Extratos Vegetais/farmacologia
2.
Int J Mol Sci ; 22(12)2021 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-34201250

RESUMO

Breast cancer (BC) is a leading cause of cancer deaths in women in less developed countries and the second leading cause of cancer death in women in the U.S. In this study, we report the inhibition of E2-mediated mammary tumorigenesis by Cuminum cyminum (cumin) administered via the diet as cumin powder, as well as dried ethanolic extract. Groups of female ACI rats were given either an AIN-93M diet or a diet supplemented with cumin powder (5% and 7.5%, w/w) or dried ethanolic cumin extract (1%, w/w), and then challenged with subcutaneous E2 silastic implants (1.2 cm; 9 mg). The first appearance of a palpable mammary tumor was significantly delayed by both the cumin powder and extract. At the end of the study, the tumor incidence was 96% in the control group, whereas only 55% and 45% animals had palpable tumors in the cumin powder and extract groups, respectively. Significant reductions in tumor volume (660 ± 122 vs. 138 ± 49 and 75 ± 46 mm3) and tumor multiplicity (4.21 ± 0.43 vs. 1.16 ± 0.26 and 0.9 ± 0.29 tumors/animal) were also observed by the cumin powder and cumin extract groups, respectively. The cumin powder diet intervention dose- and time-dependently offset E2-related pituitary growth, and reduced the levels of circulating prolactin and the levels of PCNA in the mammary tissues. Mechanistically, the cumin powder diet resulted in a significant reversal of E2-associated modulation in ERα, CYP1A1 and CYP1B1. Further, the cumin powder diet reversed the expression levels of miRNAs (miR-182, miR-375, miR-127 and miR-206) that were highly modulated by E2 treatment. We analyzed the composition of the extract by GC/MS and established cymene and cuminaldehyde as major components, and further detected no signs of gross or systemic toxicity. Thus, cumin bioactives can significantly delay and prevent E2-mediated mammary tumorigenesis in a safe and effective manner, and warrant continued efforts to develop these clinically translatable spice bioactives as chemopreventives and therapeutics against BC.


Assuntos
Cuminum/química , Estradiol/toxicidade , Estrogênios/toxicidade , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Neoplasias Mamárias Experimentais/prevenção & controle , Extratos Vegetais/farmacologia , Animais , Citocromo P-450 CYP1A1/genética , Citocromo P-450 CYP1A1/metabolismo , Citocromo P-450 CYP1B1/genética , Citocromo P-450 CYP1B1/metabolismo , Feminino , Neoplasias Mamárias Experimentais/induzido quimicamente , Neoplasias Mamárias Experimentais/patologia , MicroRNAs/genética , Ratos , Ratos Endogâmicos ACI
3.
Biomed Res Int ; 2021: 5577594, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34235220

RESUMO

Objective: Alzheimer's disease is a progressive, age-related, and neurodegenerative disease characterized by mental decline. The exact cause of Alzheimer's disease is unclear, but cholinergic dysfunction, protein accumulation, and oxidative stress are among the most important hypotheses. The main purpose of our study was to investigate the effects of aqueous and hydroalcoholic extract combination of these two medicinal plants, black pepper and cumin (as a related formulation in traditional Persian medicine), on memory and learning of an immobilized stress animal model. Methods: In this study, hydroalcoholic and aqueous extracts of cumin and black pepper fruits were prepared. Six groups of mice were treated orally for 2 weeks: control group, immobility stress, and stress-induced immobility mice received different doses of the hydroalcoholic extract (100 and 200 mg/kg) and aqueous extract (100 and 200 mg/kg). The shuttle box, novel object detection, and rotarod test were used to evaluate memory and learning. The activities of acetylcholinesterase, catalase (CAT), and superoxide dismutase (SOD) and the level of reduced glutathione (GSH) and malondialdehyde (MDA) were measured in the brain tissue. Results: Immobility stress significantly reduced learning and motor coordination. Furthermore, MDA levels and acetylcholinesterase activity were significantly increased, while CAT and SOD activities were significantly reduced in the brain of immobility-induced stress mice. Other findings indicated that hydroalcoholic and aqueous extracts (100 and 200 mg/kg) of cumin and black pepper fruits have an improving effect on animal motor coordination and learning ability, GSH content, and CAT, SOD, and acetylcholinesterase enzyme function in comparison with stress groups (p < 0.05). Conclusion: The hydroalcoholic and aqueous extracts of cumin and black pepper fruits have protective effects against stress-induced memory deficit and oxidative stress and may have beneficial therapeutic effect in the treatment of neurodegenerative diseases.


Assuntos
Doença de Alzheimer/tratamento farmacológico , Apiaceae/metabolismo , Transtornos da Memória/tratamento farmacológico , Piper nigrum/metabolismo , Acetilcolinesterase/metabolismo , Álcoois/química , Animais , Aprendizagem da Esquiva , Capsicum/química , Catalase/metabolismo , Cuminum/química , Modelos Animais de Doenças , Glutationa/metabolismo , Imobilização , Irã (Geográfico) , Peroxidação de Lipídeos , Medicina Tradicional , Camundongos , Estresse Oxidativo , Extratos Vegetais/farmacologia , Estresse Mecânico , Estresse Psicológico , Superóxido Dismutase/metabolismo
4.
Spectrochim Acta A Mol Biomol Spectrosc ; 260: 119956, 2021 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-34049008

RESUMO

Cumin (Cuminum cyminum) and fennel (Foeniculum vulgare) are widely used seasonings and play a very important role in industries such as breeding, cosmetics, winemaking, drug discovery, and nano-synthetic materials. However, studies have shown that cumin and fennel from different regions not only differ greatly in the content of lipids, phenols and proteins but also the substances contained in their essential oils are also different. Therefore, realizing precise identification of cumin and fennel from different regions will greatly help in quality control, market fraud and production industrialization. In this experiment, cumin and fennel samples were collected from each region, a total of 480 NIR spectra were collected. We used deep learning and traditional machine learning algorithms combined with near infrared (NIR) spectroscopy to identify their origin. To obtain the model with the best generalization performance and classification accuracy, we used principal component analysis (PCA) to reduce spectral data dimensionality after Rubberband baseline correction, and then established classification models including quadratic discriminant analysis based on PCA (PCA-QDA) and multilayer perceptron based on PCA (PCA-MLP). We also directly input the spectral data after baseline correction into convolutional neural networks (CNN) and generative adversarial networks (GAN). The experimental results show that GAN is more accurate than the PCA-QDA, PCA-MLP and CNN models, and the classification accuracy reached 100%. In the cumin and fennel classification experiment in the same region, the four models achieve great classification results from three regions under the condition that all model parameters remain unchanged. The experimental results show that when the training data are limited and the dimension is high, the model obtained by GAN using competitive learning has more generalization ability and higher classification accuracy. It also provides a new method for solving the problem of limited training data in food research and medical diagnosis in the future.


Assuntos
Cuminum , Foeniculum , Aprendizado de Máquina , Redes Neurais de Computação , Espectroscopia de Luz Próxima ao Infravermelho
5.
Phytother Res ; 35(9): 5007-5030, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33893678

RESUMO

Cuminum cyminum L. is a versatile spice belonging to family Apiaceae. Though the plant has pan-tropical distribution but it is indigenous to Egypt, the Mediterranean, and South Asian countries. It exhibits numerous culinary, traditional, and pharmacological attributes. Its traditional uses also validate its immense pharmacological potential. Cuminum cyminum is the hub of numerous bioactives such as alkaloids, flavonoids, terpenoids, and so forth. Cuminaldehyde is the major bioactive, rendered to most of its pharmacological as well as clinical significance. The present study comprised of current knowledge on its taxonomy, nutritional, traditional, phytochemistry, pharmacology (antimicrobial, antioxidant, anti-inflammation, antidiabetic, wound healing, anticancer, etc.), toxicology, and clinical attributes. Besides, the mechanism of action is also well explained. The present study provides a rationale for further bioprospection of this wonder plant. Future studies are needed to fill the research gaps, particularly on relevant phytocompound isolation, their pre-clinical and clinical characterization, evaluation, and structure-activity relationship. Moreover, well-designed and highly appropriate clinical and placebo trials are still needed to demonstrate the trustworthy role of cumin on human health.


Assuntos
Cuminum , Compostos Fitoquímicos/farmacologia , Cuminum/química , Humanos , Extratos Vegetais/farmacologia , Especiarias/análise
6.
Poult Sci ; 100(5): 101005, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33765487

RESUMO

The antibiotic residues and pathogenic resistance against the drug are very common in poultry because of antibiotics used in their feed. It is necessary to use natural feed additives as effective alternatives instead of a synthetic antibiotic. This study aimed to investigate the immune response of Nigella sativa and Curcuma longa in broilers under biological stress against Pasteurella multocida. The total 100, one-day-old chicks were divided into 5 groups. Groups 1 and 2 served as control negative and control positive. Both control groups were receiving simple diet without any natural feed additives, but the infection was given in group 2 at day 28 with the dose of 5.14 × 107 CFU by IV. Groups 3A and 3B were offered 2% seed powder of Nigella sativa, groups 4A and 4B were offered C. longa 1% in powdered form, and group 5A and 5B were offered both C. longa 1% and N. sativa 2% in the feed from day 1 and groups 3B, 4B, and 5B were challenged with P. multocida. The haemagglutination inhibition titter against Newcastle Disease virus (NDV), feed conversion ratio, mortality, gross, and histopathology were studied. The results of this study revealed that hemagglutination inhibition titers against NDV were highly significant (P < 0.05) in treated groups, highest titers (3A, 6.8; 3B, 6.4; and 5A, 7.2) were obtained from treated Groups. The feed conversion ratio of N. sativa + C. longa treated groups (5A, 1.57, and 3A, 1.76) were higher than that of other nontreated groups. The gross and histopathological changes were much severe in control positive, but fewer changes were seen in treated groups. Therefore, we recommend that natural feed additives, black cumin (N. sativa) and turmeric (C. longa), act as an immune enhancer in broilers against P. multocida.


Assuntos
Cuminum , Curcumina , Nigella sativa , Pasteurella multocida , Ração Animal/análise , Animais , Galinhas , Curcuma , Suplementos Nutricionais/análise , Tecido Linfoide , Sementes
7.
Microbiol Res ; 248: 126734, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33690069

RESUMO

The diseases caused by phytopathogens account for huge economic losses in the agricultural sector. Paenibacillus polymyxa is one of the agriculturally important biocontrol agents and plant growth promoting bacterium. This study describes the antifungal potential of P. polymyxa HK4 against an array of fungal phytopathogens and its ability to stimulate seed germination of cumin and groundnut under in vitro conditions. The cumin and groundnut seeds bacterized with HK4 exhibited enhanced germination efficiency in comparison to controls. The use of HK4 as a soil inoculant significantly promoted the shoot length and fresh weight of groundnut plants in pot studies. The draft genome analysis of HK4 revealed the genetic attributes for motility, root colonization, antagonism, phosphate solubilization, siderophore production and production of volatile organic compounds. The bacterium HK4 harnessed several hydrolytic enzymes that may assist its competence in the rhizosphere. The PCR amplification and sequence analysis of the conserved region of the fusA gene amplicon revealed the ability of HK4 to produce fusaricidin. Furthermore, the LC-ESI-MS/MS of crude cell pellet extract of HK4 confirmed the presence of fusaricidin as a major antifungal metabolite. This study demonstrated the potential of HK4 as a biocontrol agent and a plant growth promoter.


Assuntos
Proteção de Cultivos/métodos , Cuminum/microbiologia , Paenibacillus polymyxa/genética , Doenças das Plantas/prevenção & controle , Antifúngicos/química , Antifúngicos/metabolismo , Antifúngicos/farmacologia , Proteínas de Bactérias/análise , Proteínas de Bactérias/metabolismo , Proteínas de Bactérias/farmacologia , Cuminum/crescimento & desenvolvimento , Depsipeptídeos/análise , Depsipeptídeos/metabolismo , Depsipeptídeos/farmacologia , Fungos/efeitos dos fármacos , Fungos/fisiologia , Genoma Bacteriano , Genômica , Espectrometria de Massas , Paenibacillus polymyxa/química , Paenibacillus polymyxa/classificação , Paenibacillus polymyxa/metabolismo , Filogenia , Doenças das Plantas/microbiologia , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/microbiologia , Brotos de Planta/crescimento & desenvolvimento , Brotos de Planta/microbiologia
8.
Phytother Res ; 35(8): 4127-4135, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33720457

RESUMO

We aimed to conduct a systematic review and meta-analysis of randomized controlled trials (RCTs) to examine the effect of cumin supplementation on markers of glycemic control in adults. A comprehensive literature search was conducted up from inception to November 2020 on PubMed, Scopus, Web of Sciences, and Cochrane electronic databases. Studies that compared the effect of cumin with placebo on fasting blood sugar (FBS), serum insulin, and homeostasis model assessment-estimated insulin resistance (HOMA-IR) index in adults were considered eligible. Weighted mean difference (WMD) (with 95% confidence intervals) for endpoints were calculated using the random-effects model. Finally, a total of eight RCT studies involving 552 participants were included in the review. The results of the meta-analysis suggest that cumin supplementation did not significantly alter serum FBS (WMD: -17.77 mg/dl; 95% CI: -36.42 to 0.87, p = .06), insulin (WMD: -0.49 Hedges' g; 95% CI: -1.19 to 0.21, p =.16) levels and HOMA-IR (WMD: -0.06; 95% CI: -0.21 to 0.10, p = 0.48) index. These results do not support the use of cumin supplementation for improving glycemic markers in adults. However, further high-quality trials are still needed to confirm these results.


Assuntos
Cuminum , Suplementos Nutricionais , Resistência à Insulina , Preparações de Plantas/uso terapêutico , Adulto , Glicemia , Cuminum/química , Índice Glicêmico , Humanos , Ensaios Clínicos Controlados Aleatórios como Assunto
9.
J Biosci ; 462021.
Artigo em Inglês | MEDLINE | ID: mdl-33737496

RESUMO

Current treatment systems for tendon injuries are very few and do not ensure complete cure. This is a serious health concern for sports persons and the aged population. It is known that the nano- or microsized particles of natural products such as jeera/cumin seed (Cuminum cyminum) has been used traditionally as a home remedy for the treatment of tendon injuries. Nevertheless, these particles are likely to perform better due to their smaller size, increased absorption and local delivery in conjunction with nanotechnology. In this context, the major objective of this study was to synthesize silver-capped nanoparticles using aqueous extract of Cuminum cyminum (CCE) and to assess their in vitro non-cytotoxic effect with the perspective of clinical application to enhance tendon tissue regeneration. The presence of phytochemicals in CCE was studied by qualitative and quantitative methods. Cuminum cyminum nanoparticles (CCNP) were synthesized by the bioreduction method using silver nitrate and the particles were characterized by X-ray diffraction analysis (XRD), Fourier Transform Infra Red Spectroscopy (FTIR), Zeta potential measurement and scanning electron microscopy (SEM). The antioxidant effect of the particles was studied using total antioxidant activity and reducing power assay. Simultaneously, primary Tenocytes were isolated from rabbit Achilles tendon by collagenase and dispase digestion/treatment and characterized for Type 1 collagen. Further, in vitro non-cytotoxicity of the CCNP in direct contact with L929 mouse fibroblast cells and primary Tenocytes, respectively, was evaluated by MTT assay. Physico-chemical characterizations confirmed the formation and stability of the nanosize of CCNP with antioxidant property. Again, MTT assay confirmed the non-cytotoxicity of CCNP with L929 fibroblasts and primary Tenocytes. CCNP may be attributed as an ideal candidate for therapeutic application towards a faster restoration of worn-out/injured tendon tissue confronted by the geriatric and athlete communities.


Assuntos
Cuminum/química , Nanopartículas Metálicas/química , Tenócitos/efeitos dos fármacos , Animais , Antioxidantes/química , Antioxidantes/farmacologia , Difusão Dinâmica da Luz , Fibroblastos/efeitos dos fármacos , Química Verde , Nanopartículas Metálicas/uso terapêutico , Camundongos , Extratos Vegetais/química , Coelhos , Sementes/química , Prata , Tenócitos/fisiologia
10.
Biol Trace Elem Res ; 199(11): 4171-4182, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33462794

RESUMO

In this study, a simple, low-cost, rapid, and eco-friendly approach for the biosynthesis of silver nanoparticles (AgNPs) using the aqueous extract of Cuminum cyminum L. (cumin) seed (CcAgNPs) was developed. Also, the anti-nociceptive properties of these synthesized AgNPs were evaluated in vivo. The CcAgNPs characterized using Ultraviolet-visible (UV-Vis) spectrophotometer, X-ray diffraction analysis (XRD), Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM), and transmission electron microscopy (TEM). The analysis of phytochemical components in the aqueous extract of cumin seeds showed high concentrations of total phenols and ascorbic acid and low concentrations of total flavonoids. The analysis of phytochemical components and FTIR spectroscopy confirmed the presence of functional groups responsible for the bioreduction of Ag+ to AgNPs. The UV-Vis absorbance spectrum of CcAgNPs showed a maximum wavelength at 442 nm. The analysis of TEM images showed a spherical shape with a size of less than 50 nm, while XRD spectra revealed the crystallinity of CcAgNPs. The analysis of anti-nociceptive properties of CcAgNPs showed that the first phase of formalin-induced pain was significantly reduced in the groups receiving 200, 500, and 1000 mg/kg CcAgNPs compared with the controls and the group receiving 300 mg/kg of sodium salicylate (SS300). The second phase of formalin pain was also significantly reduced in the groups receiving 200 and 500 mg/kg CcAgNPs compared to the controls and SS300 group. Overall, we introduced a new AgNPs synthesized from cumin seeds (CcAgNPs) and showed their anti-nociceptive properties in the formalin-induced pain.


Assuntos
Cuminum , Nanopartículas Metálicas , Animais , Formaldeído , Nociceptividade , Extratos Vegetais/farmacologia , Ratos , Prata , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
11.
Int J Food Microbiol ; 341: 109047, 2021 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-33515813

RESUMO

Nowadays, adding biological compounds to food packaging is one of the types of active packaging. The aim of this study was to prepare a new degradable coating with free and nano-encapsulated Cumino cyminum L. essential oil (CCEO) with nanochitosan (Nch) base to evaluate the microbial, chemical and sensory properties of sardine fillet samples for 16 days at 4 °C. Nanoliposome using different soy lecithin ratios and cholesterol concentrations (60:0, 50:10, 40:20, and 30:30) and technique of thin-film hydration-sonication, were prepared with a range of 140-164 nm size. Encapsulation efficiency (EE) and distribution of nanoliposomes size were calculated 0.80-0.90 and 49.85-73.01% respectively. To coat sardine fillet samples, nanoliposomes with the lower size of droplet and higher EE percent were selected. The outcomes indicated that coating treatments can effectively inhibit microbial growth and chemical spoilage reflected at lower pH, peroxide value (PV) and thiobarbituric acid reactive substances (TBARs) (P < 0.05). In fact, the results of chemical and microbiological characteristics showed that the samples treated with nanocitosan/nano essential oil (NEO) showed the lowest value among other treatments during the experimental period, in the following, Nch-EO, Nch and control. pH, PV, TBARs, total viable counts (TVC), total pseudomonads count (TPC), and lactic acid bacteria (LAB) were 6.85, 0.03 (mg MDA/kg), 5.23 (mEq/kg), 3.67 (CFU/g), 3.47 (CFU/g), and 4.7 (CFU/g), respectively for Nch-NEO at the end of storage time. In addition, during the experimental period, the highest sensory properties were obtained for the Nch-NEO group. Encapsulation of CCEO reduces the rate of diffusion, thus increasing antimicrobial and antioxidant activity, as well as improving sensory properties. According to the results of this study, CCEO-encapsulated nanochitosan coatings can be used as a potent coating to increase sardine shelf life.


Assuntos
Antibacterianos/farmacologia , Quitosana/farmacologia , Cuminum/química , Embalagem de Alimentos/métodos , Óleos Voláteis/farmacologia , Alimentos Marinhos/microbiologia , Animais , Antioxidantes/química , Antioxidantes/farmacologia , Carga Bacteriana/efeitos dos fármacos , Quitosana/química , Colesterol/farmacologia , Peixes/microbiologia , Conservação de Alimentos/métodos , Armazenamento de Alimentos/métodos , Lecitinas/farmacologia
12.
Medicina (Kaunas) ; 57(1)2021 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-33406654

RESUMO

Background and Objectives: Cuminum cyminum L. has long been used in the treatment of various diseases in multiple geographical regions. This study was performed to determine the effects of C. cyminum methanolic extract (CCT) on the cellular viability, alkaline phosphatase activity and mineralization of human mesenchymal stem cells. Materials and Methods: Bone marrow-derived stem cells were cultured in the presence of CCT at concentrations of 0, 0.001, 0.01, 0.1 and 1 µg/mL. Evaluations of cell morphology were performed on days 1, 3, 7 and 14. Cellular viability was evaluated on days 1, 3, 5 and 7. On the 7th and 14th day, alkaline phosphatase activity measurements and Alizarin red S staining were conducted to assess the osteogenic differentiation of stem cells. A real-time polymerase chain reaction was used to determine the expression levels of RUNX2, BSP, OCN, COL2A1 and ß-catenin mRNAs. Results: Stem cells in the control group showed fibroblast-like morphology and the addition of CCT at 0.001, 0.01, 0.1 and 1 µg/mL did not generate noticeable changes in morphology compared with the untreated control group. The application of CCT did not produce significant changes in cellular viability or alkaline phosphatase activity compared with controls. Alizarin Red S staining was significantly increased with the application of CCT. Treatment with CCT increased the expressions of RUNX2, BSP and OCN. Conclusions: These results indicate that CCT enhanced the osteogenic differentiation of stem cells derived from bone marrow by regulating the expressions of RUNX2, BSP and OCN. Thus, the use of CCT may be applied to achieve beneficial effects on the mineralization of stem cells.


Assuntos
Cuminum , Células-Tronco Mesenquimais , Medula Óssea , Diferenciação Celular , Células Cultivadas , Humanos , Osteogênese , Células-Tronco
13.
Plant Dis ; 105(4): 1165-1167, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32990522

RESUMO

The fungal genus Alternaria consists of highly diverse species. They can be isolated readily from soil, water, and many plants, and even from animals and humans. Alternaria burnsii is a small-spored species of section Alternaria. It has been reported as a pathogen, an endophyte, and a saprophyte, and can also be found in indoor air. It causes cumin blight, a destructive disease on cumin (Cuminum cyminum), and also causes other serious diseases, such as pumpkin seed rot, date palm leaf spot, wheat leaf spot, and gray spot of Notopterygium incisum. In this study, we sequenced and assembled the first genome of A. burnsii isolate CBS 107.38. The draft genome can be used as a reference for the further study of related pathogens and comparative genomics of Alternaria species.


Assuntos
Apiaceae , Cuminum , Alternaria/genética , Genômica
14.
Eur J Pharm Sci ; 157: 105602, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33086117

RESUMO

Fungal infections by resistant Candida species continue to be a significant health problem. Novel antifungal agents such as essential oils of cumin seeds (EOCS) are tested against vulvovaginal candidiasis (VVC). The aim of this study was to develop coated polyethylene glycol (PEG) vaginal suppositories containing EOCS for treatment of VVC. PEG suppositories containing EOCS were prepared ppearance, weight variation, drug content, hardness, dissolution time, release, stability and anticandida activity were evaluated. Biocompatibility of selected formulation was tested in female rabbits, followed by clinical evaluation. Coated suppositories showed complete release of the oil after 30 min, in vitro anti-candida activity, enhanced stability and sufficient safety on the vaginal tissues of rabbits. Clinical results showed significant lower rates of vaginal itching, discharge and dyspareunia combined with negative cultures in 70% of patients, revealing efficacy of EOCS-containing vaginal suppositories for treatment of VVC.


Assuntos
Candidíase Vulvovaginal , Cuminum , Óleos Voláteis , Animais , Antifúngicos/uso terapêutico , Candidíase Vulvovaginal/tratamento farmacológico , Feminino , Humanos , Coelhos , Sementes , Supositórios
15.
Food Chem ; 338: 128143, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33091986

RESUMO

Preparation and characterization of novel encapsulation system based on calcium alginate hydrogels filled with cumin essential oil has been investigated. Firstly, the effect of sodium alginate concentration, CaCl2 level, hardening time, encapsulation and emulsion fabrication methods was studied on loading capacity of the hydrogels using a Resolution-V fractional factorial design (2 V5-1 FFD), followed by response surface methodology (RSM). At the optimum point, the in-vitro release of phenolic compounds in simulated gastric and intestinal mediums were 96.02 ± 0.96% and 10.65 ± 1.23% after 180 min, respectively. The Scanning electron microscopy (SEM) images demonstrated a relatively smooth surface with small pore size. Based on SEM images and Fourier-transform infrared spectrums, the cumin essential oil was encapsulated successfully in calcium alginate beads. Thus, calcium alginate hydrogel could be introduced as a promising carrier for encapsulating biochemical active compounds with favorable features.


Assuntos
Alginatos/química , Cuminum/química , Hidrogéis/química , Microesferas , Óleos Voláteis/química , Sementes/química
16.
Food Microbiol ; 94: 103656, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33279081

RESUMO

The objectives of this study were to investigate the effects of processing parameters (relative humidity (RH), temperature, and exposure time) on the ethylene oxide (EtO) microbial inactivation of Salmonella spp. and to evaluate Enterococcus faecium NRRL B2354 as a suitable surrogate for Salmonella inactivation on cumin seeds. Five grams of cumin seeds inoculated with either Salmonella or E. faecium were treated with EtO at different temperatures (46, 53, and 60 °C) and RH (30, 40, and 50%) levels for different exposure time to investigate the effects of process parameters on the microbial inactivation. The Weibull model fit the survival data of both bacteria with a shape parameter p < 1, which showed a tailing effect with concave shape indicating that the sensitive cells were inactivated first, and the sturdy ones survived at low RH treatment conditions. In general, the log reductions of both bacteria on cumin seeds increased with the increasing RH and temperature for EtO treatment. RH is a critical factor for successful EtO inactivation treatment. RH must be higher than 40% to implement a successful and efficient EtO decontamination of cumin seeds. E. faecium consistently showed lower log reductions than those of Salmonella under all EtO treatment conditions investigated in this study, demonstrating that E. faecium is a suitable surrogate for Salmonella. Twenty minutes of EtO treatment at 50% RH achieved ~5 log reductions of both bacteria at all three temperatures. A response surface model was developed to predict the log reductions of both bacteria under different treatment conditions and the contour plots representing log reductions were created. Inactivation is positively correlated to temperature and RH. Therefore, a higher temperature is required to achieve the desired log reduction at lower RH and vice versa. The developed response surface model is a valuable tool for the spice industry in identifying the possible combinations of EtO process parameters (temperature, RH, and exposure time) required to achieve a desired microbial reduction of Salmonella for ensuring microbial food safety of spices.


Assuntos
Cuminum/microbiologia , Enterococcus faecium/efeitos dos fármacos , Óxido de Etileno/química , Óxido de Etileno/farmacologia , Conservação de Alimentos/métodos , Salmonella enterica/efeitos dos fármacos , Contagem de Colônia Microbiana , Enterococcus faecium/crescimento & desenvolvimento , Conservação de Alimentos/instrumentação , Conservantes de Alimentos/química , Conservantes de Alimentos/farmacologia , Inocuidade dos Alimentos , Gases/farmacologia , Viabilidade Microbiana/efeitos dos fármacos , Salmonella enterica/crescimento & desenvolvimento , Sementes/microbiologia , Especiarias/microbiologia , Temperatura
17.
PLoS One ; 15(11): e0242862, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33232384

RESUMO

Bunch rot in grapes is an aggressive disease and needs to be controlled during the postharvest period. We investigate the antifungal potential of Zanthoxylum bungeanum Maxim., Zanthoxylum rhetsa, Cuminum cyminum, Coriandrum sativum, and Zingiber montanum (J. Koenig) Link ex A. Dietr. essential oils against Aspergillus aculeatus that cause bunch rot disease on postharvest grapes. C. cyminum essential oil exhibited stronger significantly inhibition percentage of 95.08% than other treatments in in vitro assay. Cumin aldehyde (33.94%) and α-terpinen-7-al (32.20%) were identified as major volatile compounds in C. cyminum oil. Antifungal potential of C. cyminum oil was then tested in conidia germination and in vitro tests compared to cumin aldehyde and α-terpinen-7-al. Their EC50 values against the conidial germination were also estimated. Significant reduction of conidia germination was also detected in C. cyminum essential oil and cumin aldehyde at a concentration of 1,000 and 100 µg/mL, respectively. EC50 values of the C. cyminum essential oil, cumin aldehyde, and α-terpinen-7-al were 67.28 µg/mL, 9.31 µg/mL, and 13.23 µg/mL, respectively. In vivo assay, the decrease of the disease severity (0.69%) and incidence (1.48%) percentage of A. aculeatus on grape berries treated at 1,000 µg/mL of C. cyminum essential oil was significantly greater than that obtained from other treatments after 10 days incubation. In addition, grape berries treated with C. cyminum essential oil decreased weight loss and retained fruit firmness. The changing of total soluble solids, total phenolic content, and antioxidant activity are also delayed in treated fruits. Therefore, essential oil of C. cyminum may be applied as a biological antifungal agent to control A. aculeatus in postharvest grapes without any negative effects on its quality.


Assuntos
Cuminum/química , Óleos Voláteis/farmacologia , Doenças das Plantas/prevenção & controle , Vitis/efeitos dos fármacos , Antifúngicos/química , Antifúngicos/farmacologia , Aspergillus/efeitos dos fármacos , Aspergillus/patogenicidade , Humanos , Óleos Voláteis/química , Doenças das Plantas/microbiologia , Vitis/crescimento & desenvolvimento , Vitis/microbiologia
18.
Exp Parasitol ; 218: 107984, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32871143

RESUMO

The fascioliasis is a parasitic disease of importance in veterinary medicine and public health. For this parasitosis, the treatment by synthetic fasciolicides is used and due to their intense use although they have been shown less effective because of the establishment of resistant Fasciola hepatica population to these drugs, with a global concern. The use of derived products of plants with biological activity has been shown promising in the control of parasites. In this context, we evaluated the chemical composition and action of ovicidal in vitro fixed oil of Helianthus annuus L. (FOH) and essential oil of Cuminum cyminum L. (EOC), as well as their combination (FOH + EOC) of F. hepatica. In the assay in vitro of F. hepatica were submitted to different concentrations of oils, such as FOH (2.3 mg/mL + 0,017 mg/mL); EOC (2.07 mg/mL + 0,004 mg/mL) and the combination of (1.15 mg/mL + 1.03 mg/mL to 0,0085 mg/mL + 0,008 mg/mL) as well as a positive control of thiabendazole (0.025 mg/mL) and a negative control with distilled water and tween. The identification of the majority chemical compounds was performed by gas chromatography. The -cell viability of the oils was tested in MDBK cellular line by the MTT method. The majority compounds in the FOH were the linoleic (53.6%) and oleic (35.85%) unsaturated fatty acids, and the majority phytochemicals compounds in the EOC were the Cumaldehyde (26.8%) and the 2-Caren 10-al (22.17%). The EOC and the combination presented effectiveness of 99% (±1) and of 94% (±1) in the concentration of 0.03 mg/mL and 0.035 mg/mL+0.03 mg/mL, respectively, and the FOH was insufficiently active as ovicidal. The cell viability at this concentration of EOC was 93%. From the results above we could infer that the EOC is promising as a new alternative for the fascioliasis control.


Assuntos
Cuminum/química , Fasciola hepatica/efeitos dos fármacos , Helianthus/química , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Análise de Variância , Animais , Anti-Helmínticos/farmacologia , Bovinos , Sobrevivência Celular/efeitos dos fármacos , Cromatografia Gasosa , Cães , Combinação de Medicamentos , Indicadores e Reagentes , Fígado/parasitologia , Células Madin Darby de Rim Canino/efeitos dos fármacos , Óleos Voláteis/química , Óvulo/efeitos dos fármacos , Óleos Vegetais/química , Sais de Tetrazólio , Tiabendazol/farmacologia
19.
Int J Biol Macromol ; 164: 1501-1509, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-32750471

RESUMO

This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage. The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria were retarded in all treated groups (Ch, GI, Ch + CNE, Ch + GI, and Ch + CNE + GI groups) compared to control group during storage time. The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins. All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples. The combination of Ch + CNE + GI was the most effective treatment to control the population of microbial flora and inoculated pathogens, slow down some physicochemical changes, and enhance the storage life of beef loins. As a result, the combination of active chitosan film and low-dose gamma irradiation can ensure microbiological safety and is suggested for long time preservation of beef during chilled storage.


Assuntos
Filmes Comestíveis , Conservação de Alimentos/métodos , Armazenamento de Alimentos/métodos , Carne/microbiologia , Carne/efeitos da radiação , Óleos Voláteis/química , Animais , Antioxidantes/química , Bovinos , Quitosana/química , Cuminum/química , Emulsões/química , Enterobacteriaceae , Escherichia coli , Contaminação de Alimentos , Microbiologia de Alimentos , Raios gama , Microbioma Gastrointestinal , Concentração de Íons de Hidrogênio , Ácido Láctico/química , Listeria monocytogenes , Nanopartículas/química , Nitrogênio/química , Tamanho da Partícula , Carne Vermelha/efeitos da radiação , Refrigeração , Salmonella typhimurium , Temperatura , Substâncias Reativas com Ácido Tiobarbitúrico/química , Fatores de Tempo
20.
Biomolecules ; 10(7)2020 07 15.
Artigo em Inglês | MEDLINE | ID: mdl-32679821

RESUMO

A new biorefinery approach has been developed in the present study, and applied on cumin (Cuminum cyminum) seeds as a potential source of phytochemicals of interest. Cumin is a popular spice used widely for its distinctive aroma. It is a rich reserve of both vegetable and essential oils. The biorefinery approach here focused on the evaluation of the influence of four different geographical origins (i.e., Lebanon, France, Algeria and Syria) on oil yield and quality in cumin seed, and on the valorization of remaining by-products by investigating their nutritional content and biological activity for the first time. Vegetable and essential oils were extracted, and their compositions were determined. Nutritional traits were also assessed. The delipidated and hydrodistillated cakes just as aromatic water were characterized for their fiber, sugar, protein, phenol and flavonoid contents. Antibacterial and antioxidant activities were also determined. Cumin seeds showed high contents in both vegetable and essential oils, proteins and sugars regardless their origin. Moreover, this Apiaceae species exhibited high levels of petroselinic fatty acid (an isomer of oleic acid) and sterols. Cakes and aromatic water also presented high levels of proteins, fibers, sugars and phenols. These residues revealed interesting antioxidant and antibacterial activities. These results emphasized the potential use of cumin in a biorefinery concept, with a multi-purpose industrial process. In addition, large differences were observed between the four geographical origins for phytochemical contents and compositions. These findings highlight the perspectives for developing selection programs for nutritional traits and industrial interests. All obtained results validate the health promoting effect of cumin composition as well as its industrial importance along with the residues.


Assuntos
Cuminum/química , Óleos Voláteis/análise , Compostos Fitoquímicos/análise , Argélia , Cuminum/classificação , França , Líbano , Valor Nutritivo , Filogeografia , Extratos Vegetais/química , Óleos Vegetais/análise , Sementes/química , Síria
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