Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 272
Filtrar
1.
Sultan Qaboos Univ Med J ; 24(2): 209-215, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38828258

RESUMO

Objectives: This study aimed to compare the effects of green cumin (Cuminum cyminum) and nettle (Urtica dioica L.) oral drops on the indicators of breast milk adequacy in lactating mothers. Due to the presence of phytoestrogens in the combinations of cumin and nettle, it is stipulated that they may have milk-increasing properties. Methods: A triple-blind, randomised, controlled clinical trial was conducted on 117 lactating mothers who had given birth to healthy infants aged 10-15 days and who received cumin oral drops (n = 39), nettle oral drops (n = 39) or placebo (n = 39) from August 2020 to March 2021. The participants were recruited from a regional public health care centre affiliated with Iran University of Medical Sciences of Tehran, Tehran, Iran. The 3 study groups received 15 drops thrice a day for 4 weeks. Infant weight, breastfeeding frequency, number of wet diapers, diaper weight and frequency of infant defecation were evaluated before and after the intervention. Results: At the beginning of the trial, no statistically significant differences were observed between the 3 groups for infant weight (P = 0.891), breastfeeding frequency (P = 0.921), number of wet diapers (P = 0.783), diaper weight (P = 0.841) and frequency of infant defecation (P = 0.898). However, following the intervention, the mean scores of all indicators were significantly higher in the experimental groups than in the placebo group (P <0.001). In addition, all the indicators in the cumin group increased significantly compared to those in the nettle group (P <0.001). Conclusion: Considering the effectiveness of cumin and nettle drops in increasing milk and the availability of these native plants in Iran, it is suggested that they, especially cumin, be used postpartum to increase breast milk production.


Assuntos
Aleitamento Materno , Cuminum , Lactação , Leite Humano , Humanos , Irã (Geográfico) , Feminino , Leite Humano/efeitos dos fármacos , Adulto , Recém-Nascido , Aleitamento Materno/estatística & dados numéricos , Aleitamento Materno/métodos , Lactação/efeitos dos fármacos , Mães/estatística & dados numéricos , Lactente
2.
Sci Rep ; 14(1): 10052, 2024 05 02.
Artigo em Inglês | MEDLINE | ID: mdl-38698117

RESUMO

The Apiaceae family contains many species used as food, spice and medicinal purposes. Different parts of plants including seeds could be used to obtain essential (EO) oils from members of the Apiaceae family. In the present study, EOs were components obtained through hydrodistillation from the seeds of anise (Pimpinella anisum), carrot (Daucus carota), celery (Apium graveolens), dill (Anethum graveolens), coriander (Coriandrum sativum), fennel (Foeniculum vulgare), and cumin (Cuminum cyminum). EO constituents were determined with Gas Chromatography/Mass Spectrometry (GC-MS) and Gas Chromatography/Flame Ionization Detector (GC-FID) and their antioxidant capacities were determined with the cupric reducing antioxidant capacity (CUPRAC) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) methods. The antimicrobial activity of EOs were tested against four pathogenic bacteria. Phenylpropanoids in anise (94.87%) and fennel (92.52%), oxygenated monoterpenes in dill (67.59%) and coriander (98.96%), monoterpene hydrocarbons in celery (75.42%), mono- (45.42%) and sesquiterpene- (43.25%) hydrocarbons in carrots, monoterpene hydrocarbon (34.30%) and aromatic hydrocarbons (32.92%) in cumin were the major compounds in the EOs. Anethole in anise and fennel, carotol in carrot, limonene in celery, carvone in dill, linalool in coriander, and cumin aldehyde in cumin were predominant compounds in these EOs. The high hydrocarbon content in cumin EO gave high CUPRAC activity (89.07 µmol Trolox g-1), and the moderate monoterpene hydrocarbon and oxygenated monoterpene content in dill EO resulted in higher DPPH activity (9.86 µmol Trolox g-1). The in vitro antibacterial activity of EOs against Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli was evaluated using the agar diffusion method and the minimum bactericidal concentration was determined. Coriander, cumin and dill EOs showed inhibitory effect against all tested strains except P. aeruginosa. While fennel and celery EOs were effective against E. coli and B. cereus strains, respectively, anise and carrot EOs did not show any antibacterial effect against the tested bacteria. Hierarchical Cluster Analysis (HCA) produced four groups based on EO constituents of seven species. The potential adoption of the cultivated Apiaceae species for EO extraction could be beneficial for the wild species that are endangered by over collection and consumption.


Assuntos
Antioxidantes , Apiaceae , Daucus carota , Foeniculum , Óleos Voláteis , Óleos Voláteis/farmacologia , Óleos Voláteis/química , Antioxidantes/farmacologia , Antioxidantes/química , Antioxidantes/análise , Apiaceae/química , Daucus carota/química , Foeniculum/química , Cuminum/química , Cromatografia Gasosa-Espectrometria de Massas , Antibacterianos/farmacologia , Antibacterianos/química , Testes de Sensibilidade Microbiana , Coriandrum/química , Sementes/química , Anethum graveolens/química , Pimpinella/química , Óleos de Plantas/farmacologia , Óleos de Plantas/química , Apium/química
3.
Skin Res Technol ; 30(5): e13727, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38711343

RESUMO

Wound healing is a complex, intricate, and dynamic process that requires effective therapeutic management. The current study evaluates the wound healing potentials of methanolic extract of Cuminum cyminum L. seeds (CCS) in rats. Sprague Dawley (24) rats were distributed into four cages, wounds produced on the back of the neck, and received two daily topical treatments for 14 days: A, rats received normal saline; B, wounded rats treated with intrasite gel; C and D, rats received 0.2 mL of 250 and 500 mg/kg of CCS, respectively. After that, wound area and closure percentage were evaluated, and wound tissues were dissected for histopathological, immunohistochemical, and biochemical examinations. Acute toxicity trials of methanolic extract of CCS showed the absence of any physiological changes or mortality in rats. CCS application caused a significant reduction in wound size and a statistically elevated percentage of wound contraction than those of vehicle rats. CCS treatment caused significant up-regulation of collagen fiber, fibroblasts, and fewer inflammatory cells (inflammation) in granulation tissues. TGF-ß1 (angiogenetic factor) was significantly more expressed in CCS-treated rats in comparison to normal saline-treated rats; therefore, more fibroblasts transformed into myofibroblasts (angiogenesis). CCS-treated rats showed remarkable antioxidant potentials (higher SOD and CAT enzymes) and decreased MDA (lipid peroxidation) levels in their wound tissue homogenates. Hydroxyproline amino acid (collagen) was significantly up-regulated by CCS treatment, which is commonly related to faster wound closure area. The outcomes suggest CCS as a viable new source of pharmaceuticals for wound treatment.


Assuntos
Cuminum , Extratos Vegetais , Ratos Sprague-Dawley , Sementes , Cicatrização , Animais , Cicatrização/efeitos dos fármacos , Sementes/química , Ratos , Extratos Vegetais/farmacologia , Cuminum/química , Masculino , Pele/lesões , Pele/efeitos dos fármacos , Pele/patologia , Fator de Crescimento Transformador beta1/metabolismo
4.
Sci Rep ; 14(1): 7990, 2024 04 05.
Artigo em Inglês | MEDLINE | ID: mdl-38580717

RESUMO

One of the significant aromatic plants applied in food and pharma is cumin. Despite its massive trading in Egypt, there are no comprehensive reports on cumin landraces profile screening. This study aimed to investigate the variation in seeds' physical and biochemical profiles and genetic diversity as well as assess the efficiency of seeds' germination under salinity stress. Consequently, during the 2020/2021 growing season, four common cumin seed landraces were gathered from various agro-climatic regions: El Gharbia, El Menia, Assiut, and Qena. Results showed a significant variation in physical profile among the four seeds of landraces. In addition, Assiut had the highest percentage of essential oil at 8.04%, whilst Qena had the largest amount of cumin aldehyde, the primary essential oil component, at 25.19%. Lauric acid was found to be the predominant fatty acid (54.78 to 62.73%). According to ISSR amplification, El Menia presented a negative unique band, whereas other landraces offered a positive band. Additionally, the cumin genotypes were separated into two clusters by the dendrogram, with El Gharbia being located in an entirely separate cluster. There were two sub-clusters within the other cluster: El Menia in one and Assiut and Qena in the other. Moreover, the germination sensitivity to the diverse salinity concentrations (control, 4, 8, 12, and 16 dS/m) findings showed that landraces exhibited varying responses to increased salinity when El Gharbia and El Menia showed a moderate response at four dS/m. Whilst, Qena landraces showed supreme values among other landraces under 12 and 16 dS/m. The majority of the examined features had strong positive associations over a range of salinity levels, according to phenotypic correlation coefficient analysis. To accomplish the aims of sustainable agriculture in Egypt, it would be imperative that the potential breeding program for cumin landraces consider this screening study.


Assuntos
Cuminum , Óleos Voláteis , Egito , Melhoramento Vegetal/métodos , Genótipo
5.
Sci Total Environ ; 923: 171497, 2024 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-38453091

RESUMO

Lead (Pb) can disrupt plant gene expression, modify metabolite contents, and influence the growth of plants. Cuminum cyminum L. is highly adaptable to adversity, but molecular mechanism by which it responds to Pb stress is unknown. For this study, transcriptomic and metabolomic sequencing was performed on root tissues of C. cyminum under Pb stress. Our results showed that high Pb stress increased the activity of peroxidase (POD), the contents of malondialdehyde (MDA) and proline by 80.03 %, 174.46 % and 71.24 %, respectively. Meanwhile, Pb stress decreased the activities of superoxide dismutase (SOD) and catalase (CAT) as well as contents of soluble sugars and GSH, which thus affected the growth of C. cyminum. In addition, Pb stress influenced the accumulation and transport of Pb in C. cyminum. Metabolomic results showed that Pb stress affected eight metabolic pathways involving 108 differentially expressed metabolites, primarily amino acids, organic acids, and carbohydrates. The differentially expressed genes identified through transcriptome analysis were mainly involved the oxidation reductase activity, transmembrane transport, phytohormone signaling, and MAPK signaling pathway. The results of this study will help to understand the molecular mechanisms of C. cyminum response to Pb stress, and provide a basis for screening seeds with strong resistance to heavy metals.


Assuntos
Antioxidantes , Cuminum , Antioxidantes/metabolismo , Cuminum/química , Cuminum/metabolismo , Chumbo/toxicidade , Metabolômica , Perfilação da Expressão Gênica
6.
J Sci Food Agric ; 104(9): 5001-5009, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38268087

RESUMO

BACKGROUND: Cumin (Cuminum cyminum L.) is one of the most important medicinal plants, and its essential oil (EO) varies between 2.5% to 5% depending on differences in climate. The extraction method plays a significant role in the market price of EOs. In this study, the effect of atmospheric cold plasma (ACP) pretreatments (using air and argon (Ar) gases) for different times on the EO yield and on the quality, color, surface morphology, and wettability of cumin seeds were studied. RESULTS: The scanning electron microscope analysis results revealed that the formation of fissures and cracks caused by ACP pretreatments was directly related to increasing the efficiency of EO extraction. Comparing the two gas treatments, the highest total color changes ΔE were related to the Ar and the lowest to the air treatment, and the highest amount of browning index was related to the Ar ACP pretreatment. In general, the ACP pretreatments improved the extraction efficiency compared with the control, so that the highest increase was observed in the Ar ACP pretreatment at the rate of 44%. Ar ACP pretreatments were observed to have a higher extraction efficiency than air ACP did. In the Ar ACP-treated samples, cumin aldehyde, as the most important component of EO, was increased compared with the control (47.9-56.4%). CONCLUSION: The data obtained in this study showed that ACP pretreatment of cumin seeds could increase EO extraction efficacy. Thus, ACP could be a promising technique to enhance the cumin seed EO extraction. © 2024 Society of Chemical Industry.


Assuntos
Cuminum , Óleos Voláteis , Gases em Plasma , Sementes , Cuminum/química , Óleos Voláteis/química , Óleos Voláteis/isolamento & purificação , Gases em Plasma/química , Sementes/química , Óleos de Plantas/química , Óleos de Plantas/isolamento & purificação , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação
7.
Anal Methods ; 16(4): 524-536, 2024 01 25.
Artigo em Inglês | MEDLINE | ID: mdl-38168938

RESUMO

A novel porous polydimethylsiloxane/bimetallic ZnCo-MOF carbonization (PDMS/ZnCo-MOF@C) sponge was successfully fabricated, followed by its utilization in GC-MS for the high efficiency extraction and determination of volatile compounds in cumin. The PDMS/ZnCo-MOF@C sponge exhibits outstanding properties with a considerable adsorption capacity, high surface area, and large pore volume and has shown potential as an ideal adsorbent for the separation and preconcentration of trace volatile compounds. The effect of different parameters on the extraction efficiency were investigated. Excellent analytical performances were achieved for the representative compounds (ß-pinene, p-cymene, γ-terpinene, cuminaldehyde, and linalyl acetate), including wide linearity (2.31-440.1 ng) with high correlation coefficients (R2 ≥ 0.9979), low LODs (1.02-3.11 ng) and LOQs (2.45-7.08 ng), and satisfactory precision (intra-day RSDs ≤ 2.89% and inter-day RSDs ≤ 4.14%). The optimal method was applied for the analysis of cumin from different regions and 44 volatile compounds were identified. The correlation between the different regions of cumin and volatile compounds was explored using multivariate statistical analysis. These results demonstrated that PDMS/ZnCo-MOF@C is an efficient, simple and sensitive material for use in the pretreatment technique for the determination of the volatile compounds in aromatic plants.


Assuntos
Cuminum , Cromatografia Gasosa-Espectrometria de Massas , Dimetilpolisiloxanos
8.
Infect Disord Drug Targets ; 24(2): e251023222677, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-37885111

RESUMO

The recent outbreaks of Nipah viral infection were associated with severe respiratory illness, lethal encephalitis, and an extremely high mortality rate. As there are no approved antiviral medications, patients with NiV infections are currently treated with repurposed or investigational antivirals and supportive care. This review examines the potential health benefits of N. sativa in the treatment of NiV infection. To identify relevant studies, the literature was searched in online databases like Medline/PubMed, Google Scholar, Science Direct, and reference lists. Through its antiviral, anti-inflammatory, antioxidant, anticonvulsant, immunomodulatory, bronchodilatory, and other properties, a number of studies have demonstrated that N. sativa is effective against a variety of viral infections, inflammatory conditions, neurological and respiratory illnesses, and other conditions. As a result, in the treatment of NiV-infected patients, N. sativa could be added as an adjuvant treatment alongside repurposed or investigational antivirals and supportive care. The efficacy of N. sativa in the treatment of NiV infection will be determined by the results of upcoming randomized controlled clinical trials.


Assuntos
Cuminum , Infecções por Henipavirus , Nigella sativa , Humanos , Extratos Vegetais/uso terapêutico , Infecções por Henipavirus/tratamento farmacológico , Sementes , Antivirais/uso terapêutico
9.
Chem Biodivers ; 20(12): e202301268, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37843082

RESUMO

Interstitial Cystitis (IC) is a chronic inflammatory disease that lacks effective treatment. The present study aimed to investigate the potential of aqueous ethanol extract of Cuminum cyminum (AEECC) on oxidative stress, inflammation and overactivity of urinary bladder induced by cyclophosphamide (CYP). Female Sprague-Dawley rats received intraperitoneal administration of cyclophosphamide (150 mg/kg, i. p. 1st , 4th , and 7th days). To investigate the urothelial damage, the bladder weight, nociception behavior, and Evans blue dye extravasation method was used. The antioxidants CAT, GPX and NO were measured. ELISA determined the IL-6 and TNF-α levels. The spasmolytic effect of AEECC was investigated on isolated bladder strips and its mechanisms were determined. The enhanced nociception behavior, bladder weight, vascular permeability, edema, hemorrhage, nitric oxide, IL-6 and TNF-α levels by CYP administration were significantly reduced by AEECC (250 and 500 mg/kg). A significant increase in serum antioxidant system such as CAT and GPx was also observed in AEECC-treated rats. The AEECC (3 mg/ml) significantly reduced urinary bladder tone in the strips pre-contracted with carbachol in both control and CYP-treated rats. This relaxation was demolished by atropine, nifedipine, glibenclamide, and indomethacin but not with propranolol. The plant extract showed the presence of antioxidant and anti-inflammatory phytochemicals. These results suggest that Cuminum cyminum offers uroprotective activity and can ameliorate CYP-induced bladder toxicity by modulating antioxidant parameters, pro-inflammatory cytokine levels and bladder smooth muscle overactivity. The in silico binding interactions of antioxidant 2I3Y and anti-inflammatory protein 1TNF with various ligands from Cuminum cyminum seeds revealed potential bioactive compounds with promising antioxidant and anti-inflammatory properties, providing valuable insights for drug development and nutraceutical research.


Assuntos
Cuminum , Cistite , Ratos , Animais , Bexiga Urinária , Antioxidantes/farmacologia , Antioxidantes/uso terapêutico , Cistite/induzido quimicamente , Cistite/tratamento farmacológico , Citocinas , Fator de Necrose Tumoral alfa , Interleucina-6 , Ratos Sprague-Dawley , Ciclofosfamida/toxicidade , Anti-Inflamatórios/farmacologia
10.
Exp Parasitol ; 252: 108587, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-37454922

RESUMO

Fasciola hepatica, the liver trematode, infects ruminants and causes economic loss. Because parasites are developing resistance to commercial drugs, the negative effects of parasitism are increasing. In this study, we aimed to evaluate the efficacy of cumin (Cuminum cyminum) essential oil against F. hepatica eggs and adults. The eggs were incubated with eight concentrations of the essential oil (0.031125-4.15 mg/mL), and viable eggs were counted after 14 days and classified as embryonated or non-embryonated. Adult flukes were incubated in Roswell Park Memorial Institute medium to ensure their viability and then incubated in essential oil. They were observed for 24 h after treatment. The adults were assessed with the two lowest effective oil concentrations used in the ovicidal test. Three controls were used for both tests: nitroxynil, a negative control, and Tween®80. After incubation in oil, the adult specimens were processed for histological analysis and stained with hematoxylin-eosin. In addition, the oil was tested for cytotoxicity using Madin-Darby bovine kidney cells to assess any possible effect on them. The oil was effective in ovicidal and adulticidal inhibition of the trematode, with statistically significant results. All concentrations assessed in the ovicidal test were 100% effective. The adult test was effective within 15 h and inactivated all the specimens at the highest concentration evaluated (0.06225 mg/mL). Histological analysis showed that cumin essential oil resulted in marked areas of vacuolization. The spines showed no structural changes but were surrounded by microvesicles. These findings indicated that cumin oil could be a potential compound in the control of fasciolosis.


Assuntos
Anti-Helmínticos , Cuminum , Fasciola hepatica , Fasciolíase , Óleos Voláteis , Bovinos , Animais , Fasciolíase/tratamento farmacológico , Fasciolíase/veterinária , Fasciolíase/parasitologia , Cuminum/química , Anti-Helmínticos/farmacologia , Anti-Helmínticos/uso terapêutico , Óleos Voláteis/química
11.
Turk J Med Sci ; 53(1): 40-50, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36945945

RESUMO

BACKGROUND: Paclitaxel is a widely used drug for the treatment of cancer, but it possesses toxic effects on male reproductive system. Administering paclitaxel with an antioxidant has become a strategy for preventing the side effects of paclitaxel. Although curcumin is an antioxidant, data concerning the effect of curcumin on paclitaxel-induced testis tissue are lacking. The present study was established to examine the protective impact of curcumin against testicular damage induced by paclitaxel. METHODS: In the study, 40 Wistar albino male rats were used and randomly divided into 4 groups (n:10). The control group received only saline solution; the curcumin group received curcumin throughout the experiment; the paclitaxel group received a total of four doses of paclitaxel on days 1, 7, 14, and 21 of the experiment; curcumin + paclitaxel group received curcumin throughout the experiment and a total of four doses of paclitaxel on days 1, 7, 14, and 21 of the experiment. At the end of the experiment, the rats were decapitated under xylazine and ketamine anesthesia and their testicles were removed. The sections obtained from the testicles were stained with Hematoxylin & Eosin and histopathological damage was evaluated. The TUNEL method was applied to determine apoptotic cells. Testosterone levels were measured in the blood serum. The Johnsen testicular biopsy score (JTBS) was used to evaluate testicular tubules. DNA damage was evaluated in sperm samples taken from the ductus epididymis using the comet assay technique. RESULTS: Testicular tissue was severely damaged in the paclitaxel group. In the curcumin + paclitaxel group, it was determined that the administration of curcumin with paclitaxel reduced the histological damage in the testicular tissue. Moreover, according to the JTBS, the value was significantly higher in the testicular tubules (p < 0.05). Testosterone levels were higher in curcumin + paclitaxel group than in paclitaxel group. DNA damage also decreased significantly in curcumin + paclitaxel group when compared to paclitaxel group (p < 0.05). DISCUSSION: The results showed that curcumin may be protective against damage caused by paclitaxel in the testicles of rats.


Assuntos
Cuminum , Curcumina , Ratos , Masculino , Animais , Testículo , Antioxidantes/farmacologia , Curcumina/farmacologia , Cuminum/metabolismo , Ratos Wistar , Paclitaxel/metabolismo , Paclitaxel/farmacologia , Estresse Oxidativo , Sementes/metabolismo , Dano ao DNA , Testosterona
12.
Meat Sci ; 200: 109173, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-37001444

RESUMO

Microbial pollution and fat oxidation are the main factors that induce the deterioration in the quality of chilled fresh mutton. This study evaluated the effects of cumin (Cuminum cyminum) essential oil (CEO), Zanthoxylum essential oil (ZEO), and blended cumin/zanthoxylum essential oil (BEO) on the antibacterial, preservation of freshness, and flavor improvement of chilled fresh mutton. The results show that BEO exerts a good inhibition effect on microbial growth, lipid oxidation, and the formation of TVB-N, as well as slowing down the rate of juice loss under chilled conditions. GC-IMS assay results showed that BEO can enrich the flavor of roasted mutton with a higher level of volatile organic substances, such as ethyl acetate D. In conclusion, BEO treatments were more efficient than single treatments in ensuring the quality of lamb to improve microbiological safety and improve the flavor of roasted lamb stored under chilled conditions. Overall research indicates that BEO is an effective natural addition that can be used to preserve the quality and safety of chilled fresh mutton during storage.


Assuntos
Cuminum , Óleos Voláteis , Carne Vermelha , Zanthoxylum , Animais , Antibacterianos/farmacologia , Óleos Voláteis/farmacologia , Oxirredução , Ovinos
13.
Anal Methods ; 15(6): 849-858, 2023 02 09.
Artigo em Inglês | MEDLINE | ID: mdl-36722988

RESUMO

A novel approach based on polydimethylsiloxane/graphene oxide/deep eutectic solvent (PDMS/GO/DES) sponge headspace solid phase extraction followed by GC-MS was successfully developed to determine the volatile components in cumin. The PDMS/GO/DES exhibits outstanding properties with high adsorption capacity and good chemical stability, and has shown its potentiality as an ideal adsorbent for the extraction of volatile compounds. The influence factors of the extraction process were investigated. Excellent analytical performances were achieved, including wide linearity (0.60-107.72 ng) with high correlation coefficients (R2 ≥ 0.9951), low LODs (0.23-9.23 ng) and LOQs (0.54-18.47 ng), satisfactory precision (intra-day RSDs ≤ 2.85% and inter-day RSDs ≤ 3.92%). Under the optimal extraction conditions, the volatile components in 17 cumin samples from four origins in Xinjiang were analyzed and 31 compounds were identified. PCA was used to establish the relationship between the origins and the volatile compounds for further discriminant analysis. The results showed that the PDMS/GO/DES method was a rapid, simple and sensitive technique for the analysis of volatile components in spices.


Assuntos
Cuminum , Cromatografia Gasosa-Espectrometria de Massas/métodos , Solventes , Micro-Ondas , Extração em Fase Sólida/métodos , Dimetilpolisiloxanos
14.
Int J Environ Health Res ; 33(2): 158-169, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34889124

RESUMO

The demand for natural agents instead of chemicals in terms of food and health safety is increasing day by day. This study aimed to investigate the potential of the methanolic extract of Cuminum cyminum (C. cyminum) in the fight against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus)and Candida (C. albicans). The chemical composition of the methanolic extract of C. cyminum was analyzed using GS-MS. Also, Kováts retention indices were calculated for the detected compounds using an applicable formula. The most basic substance was cuminic aldehyde (27.86%) and p-(Dimethoxymethyl)-isopropylbenze (18.32%). The Minimum Inhibitory Concentration (MIC) of the extract was 0.1 g/mL for S. aureus and C. albicans while it was > 0.1 for E. coli. Although the methanol extract of C. cyminum acts against all three microorganisms, the most lasting effect was on S. aureus, indicating that it can be recommended as a strong antibacterial disinfectant for S. aureus.


Assuntos
Cuminum , Óleos Voláteis , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Cuminum/química , Escherichia coli , Staphylococcus aureus , Extratos Vegetais/farmacologia , Testes de Sensibilidade Microbiana
15.
Nat Prod Res ; 37(4): 681-686, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35608174

RESUMO

In this contribution, ethanolic extracts of Cuminum cyminum (C. cyminum) seeds were evaluated in terms of phytochemical content, total phenol and flavonoid contents. As far as the analytical techniques are concerned, UV-Vis, FTIR, HPLC, NMR (1H and 13C) and ESI-MS were performed. The binding capacity of five different antidiabetic enzymes was tested by in silico molecular docking studies. The HPLC, UV-Vis, FTIR, NMR and ESI-MS data highlighted the presence of seven biologically active molecules e.g. α-pinene, ß-pinene, Δ3-carene, ρ-cymene, α-terpineol, cuminaldehyde and linalool. The results coming from the in silico molecular docking studies showed that such phytochemicals present in the cumin seed extracts play an important role in the activity of key enzymes involved in carbohydrate metabolism. Therefore, C. cyminum is proven to be useful for the treatment of diabetes mellitus and its major secondary complications.


Assuntos
Cuminum , Hipoglicemiantes , Hipoglicemiantes/farmacologia , Hipoglicemiantes/análise , Cuminum/química , Simulação de Acoplamento Molecular , Compostos Fitoquímicos/farmacologia , Compostos Fitoquímicos/análise , Extratos Vegetais/química , Sementes/química
16.
Vet Res Commun ; 47(2): 861-875, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-36580224

RESUMO

This study was conducted on 600 one-day-old male broiler chicks, using a 2 × 6 factorial design (ambient temperature, A x feed additive, F), for a period of 42 days. The chicks assigned to different groups were exposed to thermoneutral (TN, 24 ± 1 °C) and constant heat stress (HT, 36 ± 1 °C) conditions, and were only fed on a basal diet, and a basal diet supplemented with either 100 mg/kg chloramphenicol, 50 IU/kg α-tocopherol, or 200, 400, and 600 mg/kg of cumin essential oil (CEO). The results showed that heat stress adversely affected performance and carcass characteristics, and increased both the mortality rate and footpad lesions. Moreover, constant chronic heat stress showed negative effects on serum biochemistry and the intestinal microbiota, increased antioxidant activity in both the plasma and breast meat, and increased counts of the pathogenic microorganisms in the small intestine. On the other hand, dietary CEO supplementation positively affected these parameters. CEO had a slight effect on performance, carcass characteristics, mortality rate and the incidence of footpad lesions. When compared to the control group, it was determined that CEO generally had a positive effect on lipid peroxidation in the plasma and tissues and decreased antioxidant enzyme activity. Furthermore, CEO positively affected serum biochemistry and counts of beneficial microorganisms in the small intestine.


Assuntos
Cuminum , Óleos Voláteis , Animais , Masculino , Antioxidantes , Galinhas , Óleos Voláteis/farmacologia , Suplementos Nutricionais , Dieta/veterinária , Resposta ao Choque Térmico , Ração Animal/análise
17.
Int J Phytoremediation ; 25(7): 840-850, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36006042

RESUMO

The occurrence of pharmaceutical pollutants in aqueous media has increased where significant research is being conducted to eliminate these toxic compounds. In the present study, Tetradecyltrimethylammonium bromide (TTAB) modified Cuminum cyminum agri-waste (CCW) was prepared to investigate the removal of diclofenac sodium (DCF) from aqueous solution in the batch process for the first time. Physical and chemical characterizations of as-prepared adsorbent were conducted using field emission scanning electron microscopy, Fourier-transform infrared spectroscopy, N2 adsorption-desorption, and point of zero charge analysis. Besides, the effect of the main parameters that affect the adsorption process, i.e., adsorbent dosage (0.25-6 g/L), contact time (0-300 min), initial DCF concentration (10-500 mg/L), and pH of the solution, were investigated. Furthermore, the resulted data were analyzed using various kinetic and isotherm models. The Pseudo-second-order model with R2 = 0.9981 showed the highest agreement with kinetic behavior. Also, the maximum adsorption capacity of DCF is 93.65 mg/g, according to the Langmuir isotherm. In acidic media, the adsorption capacity reached the highest value (44.69 mg/g). As a result, this study revealed that the agri-waste material could be modified and, as a low-cost adsorbent, have promising adsorption potential to remove pharmaceutical contaminants from the aqueous solution.


In this study, an innovative agricultural waste, Cuminum cyminum, has been used as low-cost material and modified with Tetradecyltrimethylammonium bromide cationic surfactant to remove diclofenac sodium (DCF) from aqueous solution in the batch process for the first time. TTAB-modified CCW exhibited an excellent adsorption capacity of 93.65 mg/g. Kinetic and equilibrium investigations were conducted with various models in detail.


Assuntos
Cuminum , Poluentes Químicos da Água , Diclofenaco/química , Adsorção , Poluentes Químicos da Água/química , Concentração de Íons de Hidrogênio , Biodegradação Ambiental , Termodinâmica , Água , Cinética , Espectroscopia de Infravermelho com Transformada de Fourier , Tensoativos , Preparações Farmacêuticas
18.
Anim Biotechnol ; 34(7): 2766-2777, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36052972

RESUMO

This study was carried out to evaluate the impact of cumin essential oil (CEO) supplementation on levels of certain gene expression related to antioxidant, apoptotic, detoxific, and heat shock mechanisms in the breast meat and ileum of heat-stressed broilers. The study was conducted on a 2 × 6 factorial design (heat stress + feed additive) on 600 day-old male broiler chicks for a period of 42 days. From day 7 to 42, although broilers in heat stress groups (HT) were exposed to constant chronic heat stress (36 °C), others were housed at thermoneutral ambient temperature (TN). The chicks in both conditions were fed with 6 experimental diets: C0 (basal diet with no additive), ANTIB (basal diet + 100 mg/kg chloramphenicol), VITE (basal diet + 50 IU α-tocopherol), C2 (basal diet + 200 mg/kg CEO), C4 (basal diet + 400 mg/kg CEO), C6 (basal diet+ 600 mg/kg CEO). The results showed that heat stress upregulated (except for Bcl-2) the genes related to antioxidant, apoptosis, detoxification, and heat shock mechanism. However, cumin essential oil increased the dose-dependently positive effect on certain genes in tissues of the heat-stressed broilers and downregulated (except for Bcl-2) these genes.


Assuntos
Antioxidantes , Cuminum , Animais , Masculino , Antioxidantes/farmacologia , Antioxidantes/metabolismo , Galinhas/genética , Galinhas/metabolismo , Cuminum/genética , Cuminum/metabolismo , Suplementos Nutricionais , Dieta/veterinária , Resposta ao Choque Térmico/genética , Temperatura Alta , Apoptose , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Ração Animal
19.
Molecules ; 27(24)2022 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-36558050

RESUMO

Supercritical fluid extraction is a powerful analytical tool and it is willingly used by researchers for the isolation of various components from different matrices. In our study, the carbon dioxide in the supercritical state was used for the extraction of oils from blackcurrant and black cumin seeds. To determine the optimal conditions for the process (temperature, pressure and time), the method of statistical experiment planning and the Box-Behnken design was applied and the yield of the oils and the content of fatty acids (FAs) were taken into consideration. It has been found that an increase in pressure causes an increase in extraction yield (W), and an increase in temperature, both at constant pressure and time, does not significantly change the yield value. Optimal yield values were obtained for both materials under almost similar extraction parameters: 306 bar/ 43 min/ 50 °C (blackcurrant) and 282 bar/ 40 min/ 50 °C (black cumin). The influence of the above parameters (T, p, t) on the content of FAs in the extracts has a slightly different trend. The use of supercritical carbon dioxide for the extraction of blackcurrant and black cumin seeds allowed for high process yield and high-quality, rich in polyunsaturated fatty acids oils which can be used as a substrate or final product for industry.


Assuntos
Cromatografia com Fluido Supercrítico , Cuminum , Nigella sativa , Óleos de Plantas/química , Cromatografia com Fluido Supercrítico/métodos , Dióxido de Carbono/química , Pressão , Sementes/química , Ácidos Graxos/análise , Temperatura
20.
Molecules ; 27(19)2022 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-36234940

RESUMO

Volatile organic metabolites (VOMs) present in different spices can provide distinct analytical biosignatures related to organoleptic properties and health benefits. This study aimed to establish the volatilomic fingerprint of six of the most consumed spices all over the world (saffron (Crocus sativus L.), cinnamon (Cinnamomum verum), cumin (Cuminum cyminum L.), black pepper, (Piper nigrum L.), sweet paprika (Capsicum annuum L.), and curry (a mix of different herbs and spices)). Based on headspace solid phase microextraction (HS-SPME) followed by gas chromatography-mass spectrometry (GC-MS) analysis, this is a powerful strategy to explore and establish the spice's volatile pattern and unravel the potential health benefits related to the most important VOMs identified in each spice. This comprehensive knowledge will help in the definition of their authenticity, while simultaneously protecting against potential frauds and adulterations. A total of 162 VOMs were identified. Semi-quantitative assessments revealed that terpenoids and sesquiterpenoids amounted to the major volatile class in the investigated spices, except for cinnamon, where carbonyl compounds are the major group. Most of the studied spices comprised key characteristics of aroma and health bioactive compounds, e.g., dihydrojuneol in saffron, cinnamaldehyde in cinnamon, cuminaldehyde in cumin and curry, and caryophyllene in black pepper. The principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) successfully discriminated the investigated spices, being α-cubebene, 3-methyl butanal, ß-patchoulene and ß-selinene, the most important VOMs (highest VIP's) that contributed to its discrimination. Moreover, some VOMs have a high influence on the spice's bioactive potential, helping to prevent certain diseases including cancer, inflammatory-related diseases, diabetes, and cardiovascular diseases.


Assuntos
Capsicum , Crocus , Cuminum , Piper nigrum , Compostos Orgânicos Voláteis , Crocus/química , Cuminum/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Piper nigrum/química , Especiarias/análise , Terpenos/análise , Compostos Orgânicos Voláteis/análise
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...