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1.
Allergol Immunopathol (Madr) ; 50(2): 75-77, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35257548

RESUMO

Airborne pollen is a major contributor to allergic respiratory diseases. However, the literature on patterns and seasonality of specific pollen types mainly comes from northern hemisphere and developed regions, limiting our ability to extrapolate these findings to other geographical locations. With this research letter, our aim is to contribute to the current knowledge of aerobiology by analyzing the decade-long patterns of airborne tree pollen in the city of Bahia Blanca, Argentina. In general, we found late winter to early spring as the period with the highest Cupressaceae pollen counts. In 2020, the average pollen count during July through September was 78.81 (SD 79.70), the highest recorded during the last 10 years. These months had varying pollen concentrations from moderate (15-89 grains/m3 of air) to high (90-1499 grains/m3 of air). Regardless of such variation, August had consistently been the month with the highest peak concentrations from 2010 to 2020 (61.2, SD 57.3), followed by September (27.1, SD 20.3). We did not find any month with very high (˃1500 grains/m3 of air) peak concentration. Further studies need to be done to expand our knowledge on aerobiology to characterize specific pollen sub-types and determine the exact allergenic potential of airborne pollen in different regions.


Assuntos
Cupressaceae , Árvores , Alérgenos , Brasil , Monitoramento Ambiental , Humanos , Pólen , Estações do Ano
2.
Molecules ; 27(2)2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-35056749

RESUMO

Five new dimer compounds, namely Taiwaniacryptodimers A-E (1-5), were isolated from the methanol extract of the roots of Taiwania cryptomerioides. Their structures were established by mean of spectroscopic analysis and comparison of NMR data with those of known analogues. Their antifungal activities were also evaluated. Our results indicated that metabolites 1, 2, 4, and 5 displayed moderate antifungal activities against Aspergillus niger, Penicillium italicum, Candida albicans, and Saccharomyces cerevisiae.


Assuntos
Antifúngicos/química , Antifúngicos/farmacologia , Cupressaceae/química , Raízes de Plantas/química , Antifúngicos/isolamento & purificação , Aspergillus niger/efeitos dos fármacos , Candida albicans/efeitos dos fármacos , Dimerização , Avaliação Pré-Clínica de Medicamentos , Espectroscopia de Ressonância Magnética , Metanol/química , Testes de Sensibilidade Microbiana , Estrutura Molecular , Penicillium/efeitos dos fármacos , Extratos Vegetais/química
3.
J Environ Sci (China) ; 112: 192-201, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34955203

RESUMO

Heavy metal (Cu, Mn, Zn, Pb, and Cd) concentrations were measured in the leaves of Sabina chinensis and Platycladus orientalis collected from urban, suburban, and rural sites in Tianjin, China. Photosynthetic pigment contents, reactive oxygen species content, malondialdehyde (MDA) content and antioxidant enzyme activity were investigated, providing physiological response parameters. Our comparison of the sites revealed that urbanization significantly influenced the heavy metal concentrations in both plant leaves. At the rural site, both plant leaves exhibited the lowest heavy metal accumulation. The highest Cu, Mn, and Zn concentrations were found in S. chinensis leaves from the urban site; the highest Pb and Cd concentrations were found in P. orientalis leaves from the urban site. These results indicate that the urban site contained larger heavy metal concentrations in the plant leaves that may reflect the anthropogenic emission gradient. It is also found that S. chinensis may be used to monitor airborne heavy metal pollution because it is highly quick response to heavy metals, while P. orientalis may be used for mitigation due to its high resistance. The results of this study can contribute to the development of monitoring and environmental management plans by providing information on sensitive and resistant tree species for city greening in North China.


Assuntos
Cupressaceae , Juniperus , Metais Pesados , Poluentes do Solo , China , Cidades , Cupressaceae/efeitos dos fármacos , Cupressaceae/fisiologia , Monitoramento Ambiental , Poluição Ambiental/análise , Juniperus/efeitos dos fármacos , Juniperus/fisiologia , Metais Pesados/metabolismo , Folhas de Planta/química , Poluentes do Solo/metabolismo , Árvores/efeitos dos fármacos , Árvores/fisiologia
4.
BMC Genom Data ; 22(1): 55, 2021 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-34865616

RESUMO

OBJECTIVES: Cupressaceae is the second largest family of coniferous trees (Coniferopsida) with important economic and ecological values. However, like other conifers, the members of Cupressaceae have extremely large genome (> 8 gigabytes), which limited the researches of these taxa. A high-quality transcriptome is an important resource for gene discovery and annotation for non-model organisms. DATA DESCRIPTION: Juniperus squamata, a tetraploid species which is widely distributed in Asian mountains, represents the largest genus, Juniperus, in Cupressaceae. Single-molecule real-time sequencing was used to obtain full-length transcriptome of Juniperus squamata. The full-length transcriptome was corrected with Illumina RNA-seq data from the same individual. A total of 47,860 non-redundant full-length transcripts, N50 of which was 2839, were obtained. A total of 57,393 simple sequence repeats were identified and 268,854 open reading frames were predicted for Juniperus squamata. A BLAST alignment against non-redundant protein database was conducted and 10,818 sequences were annotated in Gene Ontology database. InterPro analysis shows that 30,403 sequences have been functionally characterized against its member database. This data presents the first comprehensive transcriptome characterization of Juniperus species, and provides an important reference for researches on the genomics and evolutionary history of Cupressaceae plants and conifers in the future.


Assuntos
Cupressaceae , Juniperus , Lagartos , Animais , Cupressaceae/genética , Genômica , Juniperus/genética , Lagartos/genética , Transcriptoma/genética
5.
Molecules ; 26(22)2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34834029

RESUMO

Haematobia irritans is a cosmopolitan obligate blood-feeding ectoparasite of cattle and is the major global pest of livestock production. Currently, H. irritans management is largely dependent on broad-spectrum pesticides, which has led to the development of insecticide resistance. Thus, alternative control methods are needed. Essential oils have been studied as an alternative due to their wide spectrum of biological activities against insects. Thus, the main aim of this study was to evaluate the insecticidal, repellent and antifeedant activity of the essential oils from Blepharocalyx cruckshanksii leaves and Pilgerodendron uviferum heartwood against horn flies in laboratory conditions. The composition of the essential oils was analyzed using gas chromatography coupled to mass spectrometry. Accordingly, α-pinene (36.50%) and limonene (20.50%) were the principal components of the B. cruckchanksii essential oil, and δ-cadinol (24.16%), cubenol (22.64%), 15-copaenol (15.46%) and δ-cadinene (10.81%) were the most abundant compounds in the P. uviferum essential oil. Mortality of flies and feeding behavior were evaluated by non-choice tests, and olfactory response was evaluated using a Y-tube olfactometer. Both essential oils were toxic to horn flies, with LC50 values for B. cruckchanksii essential oil of 3.58 µL L-1 air at 4 h, and for P. uviferum essential oil of 9.41 µL L-1 air and 1.02 µL L-1 air at 1 and 4 h, respectively. Moreover, the essential oils exhibited spatial repellency in the olfactometer using only 10 µg of each oil, and these significantly reduced the horn fly feeding at all doses evaluated. Although further laboratory and field studies related to the insectistatic and insecticide properties of these essential oils against H. irritans are necessary, B. cruckshanksii leaves and P. uviferum heartwood essential oils are promising candidates for horn fly management.


Assuntos
Cupressaceae/química , Repelentes de Insetos , Muscidae/metabolismo , Myrtaceae/química , Óleos Voláteis , Folhas de Planta/química , Animais , Bovinos , Repelentes de Insetos/química , Repelentes de Insetos/farmacologia , Inseticidas/química , Inseticidas/farmacologia , Óleos Voláteis/química , Óleos Voláteis/farmacologia
6.
Plant Physiol ; 186(4): 1908-1918, 2021 08 03.
Artigo em Inglês | MEDLINE | ID: mdl-34618104

RESUMO

Root systems play a major role in supplying the canopy with water, enabling photosynthesis and growth. Yet, much of the dynamic response of root hydraulics and its influence on gas exchange during soil drying and recovery remains uncertain. We examined the decline and recovery of the whole root hydraulic conductance (Kr) and canopy diffusive conductance (gc) during exposure to moderate water stress in two species with contrasting root systems: Tanacetum cinerariifolium (herbaceous Asteraceae) and Callitris rhomboidea (woody conifer). Optical dendrometers were used to record stem water potential at high temporal resolution and enabled non-invasive measurements of Kr calculated from the rapid relaxation kinetics of water potential in hydrating roots. We observed parallel declines in Kr and gc to <20% of unstressed levels during the early stages of water stress in both species. The recovery of Kr after rewatering differed between species. T. cinerariifolium recovered quickly, with 60% of Kr recovered within 2 h, while C. rhomboidea was much slower to return to its original Kr. Recovery of gc followed a similar trend to Kr in both species, with C. rhomboidea slower to recover. Our findings suggest that the pronounced sensitivity of Kr to drought is a common feature among different plant species, but recovery may vary depending on root type and water stress severity. Kr dynamics are proposed to modulate gc response during and following drought.


Assuntos
Chrysanthemum cinerariifolium/fisiologia , Cupressaceae/fisiologia , Secas , Raízes de Plantas/fisiologia , Água/metabolismo
7.
Anal Bioanal Chem ; 413(23): 5789-5798, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34322736

RESUMO

In recent years, the invasive cypress bark beetle (Phloeosinus aubei) has caused extensive damage to Platycladus orientalis plants in China, but its infestation is hard to monitor in the early stages. In this study, gas chromatography-mass spectrometry (GC-MS) was initially employed to investigate the volatile organic compound (VOC) emissions of P. aubei-infested P. orientalis saplings. The emissions of total sesquiterpenes were dominating (84-86% of total VOCs) and increased by 3.09-fold in P. aubei-damaged P. orientalis samples compared to undamaged samples, and the monoterpenes, aromatic compounds, and ketone emissions also had varying degrees of increase between 1.39-fold and 5.65-fold. Based on this variation, gas chromatography-ion mobility spectrometry (GC-IMS) was applied, as an untargeted analytical approach, to discriminate P. orientalis samples with different invasive severity. Two different features derived from GC-IMS data were adopted as the input information for classification and prediction models. Results showed that grid search support vector machine (GS-SVM) combined with multilinear principal component analysis (MPCA) based on spectral fingerprint achieved the best classification performances (> 88.98%), and partial least squares discriminant analysis (PLSR) method can accurately predict the pest numbers (R2 > 0.9423 and RMSE < 0.9827). In a word, the VOC profiling-based approach had the potential for evaluating P. aubei invasive severity and pest management.


Assuntos
Besouros/efeitos dos fármacos , Cupressaceae/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Espécies Introduzidas , Controle de Pragas/métodos , Compostos Orgânicos Voláteis/farmacologia , Animais , China
8.
Genes (Basel) ; 12(6)2021 05 21.
Artigo em Inglês | MEDLINE | ID: mdl-34064253

RESUMO

Cryptomeria fortunei has become one of the main timber afforestation species in subtropical high-altitude areas of China due to its fast growth, good material quality, and strong adaptability, showing broad application prospects. Quantitative real-time PCR (qRT-PCR) is the most accurate and widely used gene expression evaluation technique, and selecting appropriate reference genes (RGs) is essential for normalizing qRT-PCR results. However, suitable RGs for gene expression normalization in C. fortunei have not been reported. Here, we tested the expression stability for 12 RGs in C. fortunei under various experimental conditions (simulated abiotic stresses (cold, heat, drought, and salinity) and hormone treatments (methyl jasmonate, abscisic acid, salicylic acid, and gibberellin) and in different tissues (stems, tender needles, needles, cones, and seeds) using four algorithms (delta Ct, geNorm, NormFinder, and BestKeeper). Then, geometric mean rankings from these algorithms and the RefFinder program were used to comprehensively evaluate RG stability. The results indicated CYP, actin, UBC, and 18S as good choices for studying C. fortunei gene expression. qRT-PCR analysis of the expression patterns of three target genes (CAT and MAPK1/6) further verified that the selected RGs were suitable for gene expression normalization. This study provides an important basis for C. fortunei gene expression standardization and quantification.


Assuntos
Cupressaceae/genética , Perfilação da Expressão Gênica/normas , Genes de Plantas , Resposta ao Choque Térmico , Estresse Salino , Cupressaceae/efeitos dos fármacos , Cupressaceae/metabolismo , Perfilação da Expressão Gênica/métodos , Regulação da Expressão Gênica de Plantas , Reguladores de Crescimento de Plantas/farmacologia , Reação em Cadeia da Polimerase em Tempo Real/métodos , Reação em Cadeia da Polimerase em Tempo Real/normas , Padrões de Referência
9.
Arch Microbiol ; 203(6): 3407-3413, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33890128

RESUMO

A Gram-positive, aerobic, actinobacterial strain with rod-shaped spores, CAP47RT, which was isolated from the surface-sterilized root of a native pine tree (Callitris preissii), grown in South Australia is described. The major cellular fatty acid of this strain was iso-H-C16:1 and major menaquinone was MK-8(H4). The diagnostic diamino acid in the cell-wall peptidoglycan was identified as meso-diaminopimelic acid. These chemotaxonomic data confirmed the affiliation of strain CAP47RT to the genus Pseudonocardia. Phylogenetic evaluation based on 16S rRNA gene sequence analysis placed this strain in the family Pseudonocardiaceae, being most closely related to Pseudonocardia xishanensis JCM 17906T (98.8%), Pseudonocardia oroxyli DSM 44984T (98.7%), Pseudonocardia thailandensis CMU-NKS-70T (98.7%), and Pseudonocardia ailaonensis DSM 44979T (97.9%). The results of the polyphasic study which contain genome comparisons of ANIb, ANIm, and digital DNA-DNA hybridization revealed the differentiation of strain CAP47RT from the closest species with validated names. This strain represents a novel species and the name proposed for this microorganism is Pseudonocardia pini sp. nov., indicating the source of this actinobacterium from a pine tree. The type strain is CAP47RT (= DSM 108967T = NRRL B-65534T). Genome mining revealed that this strain contained a variety of genes encoding enzymes that can degrade hazardous chemicals.


Assuntos
Cupressaceae , Raízes de Plantas , Pseudonocardia , Cupressaceae/microbiologia , Ácidos Graxos/análise , Hibridização de Ácido Nucleico , Filogenia , Raízes de Plantas/microbiologia , Pseudonocardia/classificação , Pseudonocardia/genética , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Especificidade da Espécie
10.
Arch Microbiol ; 203(6): 3341-3352, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33871674

RESUMO

An endophytic actinobacterium, strain CAP261T was isolated from the surface sterilized root of Callitris preissii (Australian native pine tree). As a result of a polyphasic taxonomy study, this strain was identified as a member of the genus Streptomyces. This strain was an aerobic actinobacterium with well-developed substrate mycelia with loop spore chains and the spore surfaces are verrucose. The closest phylogenetic members which shared the highest 16S rRNA gene sequences similarity was Streptomyces bottropensis ATCC 25435 T at 98.1%. Chemotaxonomic data including cell wall components, major menaquinones, and major fatty acids confirmed the affiliation of strain CAP261T to the genus Streptomyces. The results of the phylogenetic analysis, including physiological and biochemical studies in combination with genome comparison study, allowed the genotypic and phenotypic differentiation of strain CAP261T and the closest species with validly published names. ANIb, ANIm and dDDH values of strain CAP261T and S. bottropensis ATCC 25435 T were 86.7%, 89.2% and 33.9%, respectively. The name proposed for the new species is Streptomyces adelaidensis sp. nov. The type strain is CAP261T (= DSM 42026 T = NRRL B-24814 T).


Assuntos
Cupressaceae/microbiologia , Streptomyces/isolamento & purificação , Ácidos Graxos/análise , Genoma Bacteriano , Genótipo , Filogenia , Desenvolvimento Vegetal , Raízes de Plantas/microbiologia , Streptomyces/química , Streptomyces/classificação , Streptomyces/genética
11.
Molecules ; 26(4)2021 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-33669825

RESUMO

The aim of the present research was to determine the chemical composition and the cytotoxic effects of Tetraclinis articulata trunk bark essential oil (HEE) obtained by steam distillation and five fractions obtained by normal phase silica chromatographic separation. Chemical analysis allowed the identification of 54 known compounds. Relatively high amounts of oxygenated sesquiterpenes (44.4-70.2%) were detected, mainly consisting of caryophyllene oxide (13.1-26.6%), carotol (9.2-21.2%),14-hydroxy-9-epi-(E)-caryophyllene (3.2-15.5%) and humulene epoxide II (2.6-7.2%). The cytotoxic activity against human mammary carcinoma cell lines (MDA-MB-231) and colorectal carcinoma cell lines (SW620) of the essential oil and its fractions were assessed. All the samples displayed moderate to weak activity compared to 5-fluorouracil. The colorectal carcinoma cell line was relatively more sensitive to the essential oil and its fractions compared to the breast cancer cell line, showing IC50 values from 25.7 to 96.5 µg/mL. In addition, the essential oil and its fraction E.2 revealed a cytotoxic activity against colorectal carcinoma cell line, with IC50 values lower than 30 µg/mL. This is the first report on the chemical composition and cytotoxic activity of the trunk bark essential oil of T. articulata.


Assuntos
Cupressaceae/química , Cupressaceae/crescimento & desenvolvimento , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Casca de Planta/química , Morte Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Fracionamento Químico , Humanos , Concentração Inibidora 50 , Tunísia
12.
Mol Biol Rep ; 48(3): 3031-3036, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33759052

RESUMO

Taiwania cryptomerioides Hayata is an endangered relict tree species which is endemic to mainland China, Taiwan, Myanmar, and northern Vietnam. It is an economically important tree species and has been used for reforestation in mountain areas of mainland China and Taiwan. In order to investigate its genetic diversity for conservation and restoration, we developed and characterized 15 nuclear microsatellite markers based on next-generation sequencing data. A total of 100 microsatellite primer pairs were initially designed and tested based on the restriction-site associated DNA sequencing data. 60 of 100 loci (60%) were successfully amplified, of which 42 loci exhibited polymorphism. Fifteen polymorphic microsatellite loci with clear peaks were selected for further analyses in four T. cryptomerioides populations sampled from China (Hubei, Fujian, Guizhou, and Yunnan). The number of alleles per locus ranged from 2 to 24, and the levels of observed and expected heterozygosity ranged from 0.000 to 0.950 and from 0.000 to 0.860, respectively. This set of microsatellite markers will be useful for future population genetic studies of T. cryptomerioides in East Asia.


Assuntos
Cupressaceae/genética , Repetições de Microssatélites/genética , Polimorfismo Genético , Árvores/genética , Extremo Oriente , Loci Gênicos , Genética Populacional , Geografia , Especificidade da Espécie
13.
Sci Rep ; 11(1): 894, 2021 01 13.
Artigo em Inglês | MEDLINE | ID: mdl-33441588

RESUMO

This study was undertaken to investigate the anticancer effects of organic extracts derived from the floral cones of Metasequoia glyptostroboides. Dried powder of M. glyptostroboides floral cones was subjected to methanol extraction, and the resulting extract was further partitioned by liquid-liquid extraction using the organic solvents n-hexane, dichloromethane (DME), chloroform, and ethyl acetate in addition to deionized water. HeLa cervical and COS-7 cells were used as a cancer cell model and normal cell control, respectively. The anticancer effect was evaluated by using the Cell Counting Kit-8 assay. The viability of COS-7 cells was found to be 12-fold higher than that of the HeLa cells under the administration of 50 µg/ml of the DME extract. Further, the sub-G1 population was determined by FACS analysis. The number of cells at the sub-G1 phase, which indicates apoptotic cells, was increased approximately fourfold upon treatment with the DME and CE extracts compared with that in the negative control. Furthermore, RT-qPCR and western blotting were used to quantitate the relative RNA and protein levels of the cell death pathway components, respectively. Our results suggest that the extracts of M. glyptostroboides floral cones, especially the DME extract, which possesses several anticancer components, as determined by GC-MS analysis, could a potential natural anticancer agent.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Cupressaceae/metabolismo , Neoplasias do Colo do Útero/tratamento farmacológico , Animais , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Células COS , Chlorocebus aethiops , Feminino , Células HeLa , Humanos , Extratos Vegetais/farmacologia , Folhas de Planta/efeitos dos fármacos , Transdução de Sinais/efeitos dos fármacos , Solventes/química
14.
Nat Prod Res ; 35(15): 2616-2620, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31696737

RESUMO

The purpose of this study was to determine the chemical composition of essential oils from the aerial parts of two species belonging to the Cupressaceae family growing in the Aures region of Algeria Juniperus oxycedrus and Cupressus Sempervirens. The analysis by GC-MS and GC-FID techniques showed the presence of 38 compounds in J. oxycedrus oil where the major constituents were manoyl oxide (23.5%), pentadecan-2-enone 6Z (12.6%), abietatriene (8.0%), abieta-8,11,13-triene-7-one (6.5%), cubebol (4.6%), epi-torilenol (3.8%) and α-cadinol (2.6%), while, a total of 65 compounds were showed in C. sempervirens oil where the major constituents were α-pinene (68.0%), epi-cedrol (6.1%), α-terpenyl acetate (3.5%) and germacrene D (2.5%). It is the first time that the compounds pentadecan-2-enone 6Z, abieta-8,11,13-trien-7-one, cubebol and epi-torilenol have been identified in our J. oxycedrus and epi-cedrol in C. sempervirens essential oils with high contents, as we noticed the absence of α-pinene in our J. oxycedrus essential oil.


Assuntos
Cupressaceae , Cupressus , Juniperus , Óleos Voláteis , Argélia
15.
J Mycol Med ; 30(3): 100987, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32499133

RESUMO

Aspergillus is a fungal genus that strongly affects health of humans, animals, and plants worldwide. Endophytes are now widely considered as a rich source of bioproducts with potential uses in medicine, agriculture, and bioindustry. Cupressaceae plant family hosts a variety of bioactive ascomycetous endophytes. In this study, antifungal activity of a number of such endophytes were investigated against human pathogenic fungi Aspergillus fumigatus and Aspergillus niger. To this end, 16 superior bioactive endophytic fungi from Cupressaceae were used, including Alternaria alternata, Alternaria pellucida, Ascorhizoctonia sp., Aspergillus fumigatus, Aspergillus niger, Aurobasidium sp., Cladosporium porophorum, Fusarium oxysporum, Penicillium viridicatum, Phoma herbarum, Phoma sp., Pyrenochaeta sp., Trichoderma atroviride, Trichoderma atroviride and Trichoderma koningii. In vitro bioassays indicated anti-Asperilli activity of the endophytic fungi in dual cultures. Most notably, Trichoderma koningii CSE32 and Trichoderma atroviride JCE33 showed complete growth inhibition of both A. niger and A. fumigatus, within 3 to 7 days. Also, volatile compouds (VOCs) of T. koningii CSE32 and T. atroviride JCE33 exhibited 33-100% growth inhibition of A. niger, whithin 3 days. Moreover, on the day 7, growth of A. niger was less affected than that of A. fumigatus. In general, it appears that there is a direct relationship between the exposure time and the inhibitory activity of endophytes VOCs on the growth of target Aspergillus species. Furthremore, the extracellular secondary metabolites (SMs) of four selected fungal endophytes exhibited anti-Aspergillus activity at all treatment levels as shown by Agar-diffusion assay. SMs from T. koningii CSE32 and Pyrenochaeta CSE134 showed strongest activities against A. niger, and SMs from T. koningii CSE32 and F. oxysporum CAE14 showed strongest activities against A. fumigatus. In conclusion, given the globally recognized issue of antibiotic resistance and the urge to discover new antimicrobial substances, our findings provide new insights into the potential use of Cupressaceae's endophytic fungi in antifungal-based drug discovery programs.


Assuntos
Antibiose/fisiologia , Antifúngicos , Aspergillus fumigatus , Aspergillus niger , Cupressaceae/microbiologia , Endófitos/fisiologia , Aspergillus fumigatus/crescimento & desenvolvimento , Aspergillus fumigatus/patogenicidade , Aspergillus niger/crescimento & desenvolvimento , Aspergillus niger/patogenicidade , Humanos , Irã (Geográfico) , Testes de Sensibilidade Microbiana , Trichoderma/classificação , Trichoderma/fisiologia
16.
Clin Exp Allergy ; 50(8): 964-972, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32506709

RESUMO

BACKGROUND: Severe allergy to fruits mediated by a 7 kDa allergen belonging to the gibberellin-regulated protein (GRP) family is known to be associated with Cupressaceae pollinosis. OBJECTIVE: To identify and characterize Cupressaceae pollen allergens involved in GRP-related fruit allergy. METHODS: Pru p 7-related proteins from pollen of Cupressus sempervirens, Juniperus ashei and Cryptomeria japonica were identified using a rabbit anti-Pru p 7 antiserum, purified chromatographically and sequenced by mass spectrometry and bioinformatic comparisons. The C sempervirens protein was produced as a recombinant allergen in Pichia pastoris. IgE antibody binding to pollen GRP proteins was analysed in a peach allergic (n = 54) and a cypress pollen allergic (n = 88) patient population from southern France using ImmunoCAP. RESULTS: In each of the three Cupressaceae species studied, a 7 kDa pollen protein related to Pru p 7 was identified and found to comprise an amino acid sequence of 63 residues in length, 92%-98% identical to each other and 67%-68% identical to Pru p 7. The C sempervirens, J ashei and C japonica GRP allergens have been officially recognized by the WHO/IUIS Allergen Nomenclature Sub-Committee and named Cup s 7, Jun a 7 and Cry j 7, respectively. Recombinant Cup s 7 showed IgE antibody binding capacity comparable to that of the purified natural allergen. Among 51 peach allergic subjects sensitized to Pru p 7, substantially higher levels of IgE to Cup s 7 than to Pru p 7 were found. Further, the pollen protein was able to completely outcompete IgE binding to Pru p 7, while the reverse competition effect was modest, consistent with primary sensitization by the pollen allergen. CONCLUSION AND CLINICAL RELEVANCE: Pru p 7-related pollen allergens from three Cupressaceae species have been characterized and may become useful for the identification of pollinosis patients at risk of developing severe fruit allergy.


Assuntos
Alérgenos/imunologia , Antígenos de Plantas/imunologia , Cupressaceae/imunologia , Hipersensibilidade Alimentar/imunologia , Pólen/imunologia , Rinite Alérgica Sazonal/imunologia , Adolescente , Adulto , Idoso , Biomarcadores/sangue , Criança , Pré-Escolar , Feminino , Hipersensibilidade Alimentar/sangue , Hipersensibilidade Alimentar/diagnóstico , Humanos , Imunoglobulina E/sangue , Masculino , Pessoa de Meia-Idade , Peso Molecular , Proteínas de Plantas/imunologia , Prunus persica/imunologia , Rinite Alérgica Sazonal/sangue , Rinite Alérgica Sazonal/diagnóstico , Adulto Jovem
17.
Molecules ; 25(4)2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-32093424

RESUMO

Taiwania flousiana (T. flousiana) Gaussen is a precious wood in the family Taxodiaceae. This study investigated the chemical components of the essential oil from the stem bark of T. flousiana and its algicidal, antifungal, and antioxidant properties. Sixty-nine compounds representing 89.70% of the stem bark essential oil were identified by GC-MS. The essential oil showed strong anti-algae, anti-bacteria, and anti-fungus activities against the tested species, and antioxidant activities. The IC50 values of the essential oil against chlorophyll a, chlorophyll b, and the total chlorophyll of Spirogyra communis (a species of algae), 24-96 h after the treatment, ranged from 31.77 to 84.92 µg/mL, while the IC50 values of butachlor ranged from 40.24 to 58.09 µg/mL. Ultrastructure changes revealed by the transmission electron microscopy indicated that the main algicidal action sites were the chloroplast and cell wall. The essential oil showed antifungal activities on Rhizoctonia solani (EC50 = 287.94 µg/mL) and Colletotrichum gloeosporioiles (EC50 = 378.90 µg/mL). It also showed bactericidal activities on Ralstonia solanacearum and Staphylococcus aureus, with zones of inhibition (ZOIs) being 18.66 and 16.75 mm, respectively at 40 µg/disk. Additionally, the essential oil possessed antioxidant activity estimated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method (IC50 = 33.51 µg/mL; IC50 value of the positive control ascorbic acid was 7.98 µg/mL). Thus, the essential oil of this plant might be used as a possible source of natural bioactive molecules in agrochemical industry as well as in food and cosmetic industries.


Assuntos
Antioxidantes , Cupressaceae/química , Óleos Voláteis , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Colletotrichum/crescimento & desenvolvimento , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Ralstonia solanacearum/crescimento & desenvolvimento , Rhizoctonia/crescimento & desenvolvimento , Spirogyra/crescimento & desenvolvimento , Staphylococcus aureus/crescimento & desenvolvimento
18.
Mol Biol Rep ; 47(4): 2991-2996, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32036571

RESUMO

Metasequoia glyptostroboides is a living fossil and an endangered species listed in the International Union for Conservation of Nature (IUCN). Distinguishing the genotypes of all wild individuals of M. glyptostroboides is important to delimit management units and key germplasm resources. We characterized 28 novel polymorphic microsatellite loci using a streptavidin-biotin microsatellite-enriched library and Illumina high-throughput sequencing. Characteristics of each locus were tested using 140 individuals collected from five natural populations of M. glyptostroboides. The number of alleles per locus ranged from 3 to 20, with a mean number of about 8 alleles. The observed and expected heterozygosities in each population ranged from 0.0000 to 1.0000 and from 0.0000 to 0.8958, respectively. Four to nine loci were cross-amplified successfully in seven species of Cupressaceae. The novel SSR markers will provide a toolkit for DNA identification of all of the extant wild individuals guiding further conservation efforts of M. glyptostroboides.


Assuntos
Cupressaceae/genética , Repetições de Microssatélites/genética , Alelos , China , Conservação dos Recursos Naturais , Primers do DNA/genética , Espécies em Perigo de Extinção , Genômica/métodos , Genótipo , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Polimorfismo Genético/genética , Árvores/genética
19.
Int J Mol Sci ; 21(3)2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-32024007

RESUMO

Taiwania (Taiwania cryptomerioides) is an important tree species in Taiwan because of the excellent properties of its wood and fascinating color qualities of its heartwood (HW), as well as the bioactive compounds therein. However, limited information is available as to the HW formation of this species. The objective of this research is to analyze the differentially expressed genes (DEGs) during the HW formation process from specific Taiwania xylem tissues, and to obtain genes that might be closely associated with this process. The results indicated that our analyses have captured DEGs representative to the HW formation process of Taiwania. DEGs related to the terpenoid biosynthesis pathway were all up-regulated in the transition zone (TZ) to support the biosynthesis and accumulation of terpenoids. Many DEGs related to lignin biosynthesis, and two DEGs related to pinoresinol reductase (PrR)/pinoresinol lariciresinol reductase (PLR), were up-regulated in TZ. These DEGs together are likely involved in providing the precursors for the subsequent lignan biosynthesis. Several transcription factor-, nuclease-, and protease-encoding DEGs were also highly expressed in TZ, and these DEGs might be involved in the regulation of secondary metabolite biosynthesis and the autolysis of the cellular components of ray parenchyma cells in TZ. These results provide further insights into the process of HW formation in Taiwania.


Assuntos
Cupressaceae/genética , Perfilação da Expressão Gênica/métodos , Proteínas de Plantas/genética , Madeira/metabolismo , Cupressaceae/metabolismo , Regulação da Expressão Gênica de Plantas , Lignina/metabolismo , Metabolismo Secundário , Terpenos/metabolismo , Xilema/metabolismo
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