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1.
Comp Immunol Microbiol Infect Dis ; 93: 101943, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36610227

RESUMO

Dogs are the most cosmopolitan pets of humans and as such a means of transmitting zoonotic parasites to their owners. This study was designed to investigate the diversity, prevalence, pattern of infection, intensity of infections, and the risk factors associated with gastrointestinal parasites of dogs in Kwara Central, North Central, Nigeria. Three hundred and five clinically healthy dogs were sampled. Faecal samples were subjected to the direct smear, simple faecal centrifugation flotation, formol-ether concentration, and the Modified Ziehl-Neelsen staining techniques. Oocysts/eggs per gram of faeces were counted using the modified McMaster technique. Data were analysed using univariate logistic regression, multivariate logistic regression, and the one-way analysis of variance (ANOVA). A p -value of < 0.05 was considered significant for all analyses. One hundred and sixty-six dogs were positive for at least one species of gastrointestinal parasite, representing 54.43% (95% CI: 44.81 - 59.96) of the sampled population. The study identified Cystoisospora species (15.41%), Cryptosporidium species (25.25%), Ancylostoma species (25.25%), Toxocara canis (19.02%), Strongyloides stercoralis (7.54%), Uncinaria stenocephala (6.89%), and Dipylidium caninum (2.30%) as the gastrointestinal parasites infecting dogs in the study area. Coinfection with more than one species of gastrointestinal parasites was a common finding in dogs. The intensity of Cystoisospora spp. among infected dogs ranged between 40 and 980 oocysts per gram of faeces, while that of helminth parasites was 40 - 1560 eggs per gram of faeces. Age, sex, breeds, body condition score, presence of ticks on dogs, the purpose of keeping dog(s), types of housing, types of feed consumed, vaccination status, and treatment with antiparasitics were predators associated with the prevalence and intensity of gastrointestinal parasites infections. Due to the zoonotic nature of most of the encountered gastrointestinal parasites, there is need for regular antiparasitic treatment, proper dog management, and adequate personal hygiene to prevent zoonosis.


Assuntos
Criptosporidiose , Cryptosporidium , Doenças do Cão , Enteropatias Parasitárias , Parasitos , Animais , Cães , Humanos , Nigéria/epidemiologia , Enteropatias Parasitárias/epidemiologia , Enteropatias Parasitárias/veterinária , Enteropatias Parasitárias/parasitologia , Doenças do Cão/epidemiologia , Doenças do Cão/parasitologia , Fezes/parasitologia , Prevalência
2.
Chemosphere ; 316: 137787, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36623594

RESUMO

Environmental lead exposure poses risks to children' health, thus exposure sources and pathways identification remain important concern and research scope. Due to sharing the same environment, domestic animals, especially dogs, have been used as useful sentinels to identify human lead exposure. However, more evidence is needed on whether domestic dogs could be used to identify the lead exposure pathways and sources of children. Thus, this study investigated the dietary habits, behaviors, and household environment of children and dogs in a typical coal-fired area in China. The lead levels and lead isotope ratios (Acronym: LIRs, expressed as 208Pb/206Pb and 207Pb/206Pb) in dogs' and children's blood, as well as in environmental media (food, PM2.5, indoor/outdoor dust, drinking water and soil) were measured to explore the predominant lead pollution sources and exposure pathways of children. The results showed that the LIRs of children's blood (208Pb/206Pb = 2.0703 ± 0.0076, 207Pb/206Pb = 0.8501 ± 0.0052) were similar to those of dogs' blood (208Pb/206Pb = 2.0696 ± 0.0085, 207Pb/206Pb = 0.8499 ± 0.0052), as well as similar to the LIRs of environmental media, i.e. children's food (208Pb/206Pb = 2.0731 ± 0.0057, 207Pb/206Pb = 0.8491 ± 0.0036) and coal (208Pb/206Pb = 2.0683 ± 0.017, 207Pb/206Pb = 0.8515 ± 0.01). Children and dogs had similar lead exposure pathways, but the contributions of each exposure pathway were different, i.e., 83.1% vs. 76.9% for children and dogs via food ingestion, 1.4% vs. 5.6% via particulate matter exposure, and 15.5% vs. 17.5% via household dust exposure, respectively. The contribution of food via ingestion to lead exposure remains dominant, and coal combustion is a main lead exposure source for children and domestic dogs.


Assuntos
Exposição Ambiental , Chumbo , Humanos , Criança , Cães , Animais , Chumbo/análise , Exposição Ambiental/análise , Poeira/análise , Carvão Mineral/análise , Isótopos/análise , China , Monitoramento Ambiental/métodos
3.
Pharmacol Res Perspect ; 11(1): e01052, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36631976

RESUMO

Vinblastine (VBL) is a vinca alkaloid-class cytotoxic chemotherapeutic that causes microtubule disruption and is typically used to treat hematologic malignancies. VBL is characterized by a narrow therapeutic index, with key dose-limiting toxicities being myelosuppression and neurotoxicity. Pharmacokinetics (PK) of VBL is primarily driven by ABCB1-mediated efflux and CYP3A4 metabolism, creating potential for drug-drug interaction. To characterize sources of variability in VBL PK, we developed a physiologically based pharmacokinetic (PBPK) model in Mdr1a/b(-/-) knockout and wild-type mice by incorporating key drivers of PK, including ABCB1 efflux, CYP3A4 metabolism, and tissue-specific tubulin binding, and scaled this model to accurately simulate VBL PK in humans and pet dogs. To investigate the capability of the model to capture interindividual variability in clinical data, virtual populations of humans and pet dogs were generated through Monte Carlo simulation of physiologic and biochemical parameters and compared to the clinical PK data. This model provides a foundation for predictive modeling of VBL PK. The base PBPK model can be further improved with supplemental experimental data identifying drug-drug interactions, ABCB1 polymorphisms and expression, and other sources of physiologic or metabolic variability.


Assuntos
Antineoplásicos , Vimblastina , Humanos , Cães , Camundongos , Animais , Vimblastina/farmacocinética , Citocromo P-450 CYP3A/genética , Antineoplásicos/farmacocinética , Interações Medicamentosas , Transporte Biológico
4.
Sci Rep ; 13(1): 632, 2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36635367

RESUMO

Biological features of neoplastic disease affecting mammary gland tissue are shared between canines and humans. Research performed in either species has translational value and early phase clinical trials performed in canines with spontaneous disease could be informative for human trials. The purpose of this study was to investigate the somatic genetic aberrations occurring in canine mammary neoplasia by exome capture and next generation sequencing. Based on 55 tumor-normal pairs we identified the PIK3CA gene as the most commonly mutated gene in canine mammary tumors, with 25% of samples carrying mutations in this gene. A recurrent missense mutation was identified, p.H1047R, which is homologous to the human PIK3CA hotspot mutation found in different types of breast neoplasia. Mutations homologous to other known human mutation hotspots such as the PIK3CA p.E545K and the KRAS p.G12V/D were also identified. We identified copy number aberrations affecting important tumor suppressor and oncogenic pathways including deletions affecting the PTEN tumor suppressor gene. We suggest that activation of the KRAS or PIK3CA oncogenes or loss of the PTEN suppressor gene may be important for mammary tumor development in dogs. This data endorses the conservation of cancer across species and the validity of studying cancer in non-human species.


Assuntos
Classe I de Fosfatidilinositol 3-Quinases , Neoplasias Mamárias Animais , Animais , Cães , Feminino , Humanos , Neoplasias da Mama/genética , Classe I de Fosfatidilinositol 3-Quinases/genética , Neoplasias Mamárias Animais/genética , Mutação de Sentido Incorreto , Proteínas Proto-Oncogênicas p21(ras)/genética
5.
Arch Virol ; 168(2): 63, 2023 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-36637551

RESUMO

Influenza A virus (FLUAV) is a significant human pathogen. In silico structural analysis (PMID 28628827) has suggested that the FDA-approved drug paliperidone interferes with the binding of the FLUAV polymerase subunit PB2 to the nucleoprotein NP. We found that paliperidone inhibits FLUAV A/PR/8/34 early after infection of canine MDCK II, human A549, and human primary bronchial cells, but not at late time points. No effect was detectable against the strains A/Hamburg/05/2009 and A/WSN/33. Moreover, paliperidone indeed disturbed the interaction between the PB2 and the NP of A/PR/8/34 and reduced early viral RNA and protein synthesis by approximately 50%. Thus, paliperidone has measurable but transient and virus-strain-restricted effects on FLUAV.


Assuntos
Antivirais , Vírus da Influenza A , Palmitato de Paliperidona , Animais , Cães , Humanos , Vírus da Influenza A/efeitos dos fármacos , Vírus da Influenza A/genética , Nucleoproteínas , Palmitato de Paliperidona/farmacologia , RNA Viral , Proteínas Virais/genética , Proteínas Virais/metabolismo , Replicação Viral , Células Madin Darby de Rim Canino , Células A549 , Antivirais/farmacologia
6.
Funct Integr Genomics ; 23(1): 31, 2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36604379

RESUMO

Long-stranded noncoding RNAs (lncRNAs) play different roles in various diseases. lncRNA34977 has been shown to play a relevant role the development of canine mammary tumors (CMTs). However, the mechanism of lncRNA34977 in canine mammary tumors has not been fully investigated. The aim of this study was to investigate the effects of lncRNA34977 on the proliferation, migration, invasion, and apoptosis of canine mammary tumor (CMT) cells through the regulation of miR-8881/ELAVL4 expression. The apoptosis was detected by an in situ fluorescence assay and flow cytometry. The expression levels were analyzed by RT-qPCR. CCK-8, colony formation, wound healing, and Transwell assays were used to assess the proliferation, migration, and invasion. The expression of protein was detected by western blot. The siRNA-induced silencing of lncRNA34977 promoted the apoptosis of CHMp cells, and in overexpression of lncRNA34977, the result is the opposite. LncRNA34977 has a direct targeting relationship with miR-8881 and that miR-8881 is correlated with ELAVL4. Transfection of miR-8881 mimics inhibited the proliferation, migration, invasion, and promoted the apoptosis of CHMp cells of CHMp cells. In the transfection with miR-8881 inhibitors, the result is the opposite. Co-transfected with lncRNA34977, miR-8881, or ELAVL4, we found that lncRNA34977 could regulate the expression of miR-8881 or ELAVL4. Our study shows that lncRNA34977 promotes the proliferation, migration, and invasion and suppresses the apoptosis of CMT cells by regulating the expression of miR-8881/ELAVL4.


Assuntos
Neoplasias da Mama , MicroRNAs , RNA Longo não Codificante , Animais , Cães , Apoptose/genética , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Regulação Neoplásica da Expressão Gênica , MicroRNAs/genética , Invasividade Neoplásica/genética , Invasividade Neoplásica/patologia , RNA Longo não Codificante/genética , Glândulas Mamárias Animais , Neoplasias da Mama/genética , Neoplasias da Mama/veterinária
7.
BMC Oral Health ; 23(1): 6, 2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36604677

RESUMO

BACKGROUND: Most patients with insufficient bone mass suffer from severe horizontal or vertical bone defects in oral implant surgery. The purpose of this study was to compare the bone regeneration effects of titanium meshes with different porosity in the treatment of bone defects. METHODS: Nine beagle dogs were equally divided into three groups based on execution time. Three months after the extraction of the first to fourth premolars of the mandible, three bone defects were randomly made in the mandible. Bone particles and three kinds of three-dimensional (3D) printed titanium nets with different porosities (low porosity group (LP), 55%; medium porosity group (MP), 62%; and high porosity group (HP), 68%) were replanted in situ. The beagles were killed 4, 8, and 12 weeks after surgery. Formalin-fixed specimens were embedded in acrylic resin. The specimens were stained with micro-CT, basic fuchsin staining, and toluidine blue staining. RESULTS: Micro-CT analysis showed that the trabecular thickness, trabecular number, and bone volume fraction of the HP group were higher than those of the other two groups. Moreover, the trabecular separation of the HP group decreased slightly and was lower than that of the MP and LP groups. Histological staining analysis showed that the trabecular number in the HP group was higher than in the other two groups at 8 and 12 weeks, and the bone volume fraction of the HP was higher than that in the other two groups at 12 weeks. Moreover, the trabecular thickness of the MP was higher than that of the LP group at 12 weeks and the trabecular separation was lower in the HP group at 4 and 8 weeks. The differences were statistically significant (p < 0.05). CONCLUSION: A 3D printed titanium mesh with HP in a certain range may have more advantages than a titanium mesh with LP in repairing large bone defects.


Assuntos
Implantes Dentários , Titânio , Cães , Animais , Porosidade , Telas Cirúrgicas , Regeneração Óssea , Impressão Tridimensional
8.
Can J Vet Res ; 87(1): 74-81, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36606034

RESUMO

The objective of this study was to evaluate unidimensional (mm), bidimensional (mm2), or tridimensional (mL) computed tomography (CT) tumor measurements for ability to discriminate changes in lesion size and predict survival in dogs with nonresectable hepatic carcinoma treated with drug-eluting bead transarterial-chemoembolization (DEB-TACE) and to compare CT response via Response Evaluation Criteria in Solid Tumors 1.1 (mm), World Health Organization (mm2), ellipsoid and spherical volume (mL), and percent necrosis, for their ability to differentiate treatment responders. This was a prospective, single-arm clinical trial. DEB-TACE was performed to varying levels of blood flow stasis in 16 client-owned dogs with nonresectable hepatic carcinoma. Computed tomography imaging responses were assessed and compared to median survival time. Results revealed that initial, follow-up, or changes in unidimensional, bidimensional, or tridimensional tumor measurements were not associated with survival. Larger bidimensional and tridimensional tumor measurements/body weight on initial and follow-up CT were significantly associated with a shorter median survival time [bidimensional (P = 0.04, 0.016) and tridimensional (P = 0.025, 0.015), respectively]. A higher percent necrosis on initial CT was significantly associated with a shorter median survival time (P = 0.038). Ellipsoid volumetric criteria detected treatment response most frequently; however, response classification was not associated with median survival time. Computed tomography bidimensional and tridimensional tumor measurements/body weight before and after DEB-TACE may help to predict median survival time for dogs undergoing DEB-TACE for hepatic carcinoma.


L'objectif de cette étude était d'évaluer les mesures tumorales unidimensionnelles (mm), bidimensionnelles (mm2) ou tridimensionnelles (mL) par tomodensitométrie (CT) pour déterminer la capacité de discriminer les changements de taille des lésions et de prédire la survie chez les chiens atteints d'un carcinome hépatique non-résécable traité avec un médicament par chimioembolisation transartérielle par billes à élution (DEB-TACE) et pour comparer la réponse CT via les critères d'évaluation de la réponse dans les tumeurs solides 1,1 (mm), l'Organisation mondiale de la santé (mm2), le volume ellipsoïde et sphérique (mL) et le pourcentage de nécrose, pour leur capacité à différencier les répondeurs au traitement. Il s'agissait d'un essai clinique prospectif à un seul volet. Le DEB-TACE a été réalisé à différents niveaux de stase du flux sanguin chez 16 chiens appartenant à des clients atteints d'un carcinome hépatique non-résécable. Les réponses d'imagerie par tomodensitométrie ont été évaluées et comparées au temps de survie médian. Les résultats ont révélé que les mesures initiales, de suivi ou les modifications des mesures tumorales unidimensionnelles, bidimensionnelles ou tridimensionnelles n'étaient pas associées à la survie. Des mesures tumorales bidimensionnelles et tridimensionnelles plus grandes/poids corporel sur la CT initiale et de suivi étaient significativement associées à un temps de survie médian plus court [bidimensionnel (P = 0,04, 0,016) et tridimensionnel (P = 0,025, 0,015), respectivement]. Un pourcentage plus élevé de nécrose au scanner initial CT était significativement associé à une durée de survie médiane plus courte (P = 0,038). Les critères volumétriques ellipsoïdes ont détecté la réponse au traitement le plus fréquemment; cependant, la classification des réponses n'était pas associée à la durée médiane de survie. La tomodensitométrie bidimensionnelle et tridimensionnelle des mesures tumorales/poids corporel avant et après DEB-TACE peut aider à prédire la durée médiane de survie des chiens subissant DEB-TACE pour un carcinome hépatique.(Traduit par Docteur Serge Messier).


Assuntos
Carcinoma Hepatocelular , Quimioembolização Terapêutica , Doenças do Cão , Neoplasias Hepáticas , Animais , Cães , Carcinoma Hepatocelular/diagnóstico por imagem , Carcinoma Hepatocelular/terapia , Carcinoma Hepatocelular/veterinária , Quimioembolização Terapêutica/veterinária , Quimioembolização Terapêutica/métodos , Doenças do Cão/diagnóstico por imagem , Doenças do Cão/terapia , Neoplasias Hepáticas/diagnóstico por imagem , Neoplasias Hepáticas/terapia , Neoplasias Hepáticas/veterinária , Necrose/terapia , Necrose/veterinária , Estudos Prospectivos , Tomografia Computadorizada por Raios X/veterinária , Resultado do Tratamento
9.
Sci Rep ; 13(1): 862, 2023 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-36650191

RESUMO

Recently, double-root implants have been investigated using 3D-printed technology. Here, we investigated damping capacity, microcomputed tomographic (micro-CT) and histological analyses of double-root 3D-printed implants compared with single-root 3D printed implants. Single- and double-root 3D-printed implants were fabricated and placed at both sides of mandibular third and fourth premolars in four beagle dogs. The damping capacity was measured, and periapical X-rays were taken every 2 weeks for 12 weeks. The bone volume/tissue volume (BV/TV) and bone mineral density (BMD) around the implants were measured with micro-CT. Bone-to-implant contact (BIC) and bone area fraction occupancy (BAFO) were measured in histological samples. The implant stability values between the groups were not significantly different, except at 4 and 12 weeks. The marginal bone changes were similar at the mesial and distal areas between the groups. The BV/TV and BMD values of the double-root 3D-printed implants showed no statistical difference through micro-CT analysis, but the double-root 3D-printed implants showed lower BIC and BAFO values through histomorphometric analysis compared to the single-root 3D-printed implants. Compared to single-root implants, 3D-printed double-root implants demonstrated comparable stability and bone remodeling around the fixtures, but the statistically significant bone loss in the furcation area remains problematic.


Assuntos
Implantes Dentários , Osseointegração , Cães , Animais , Titânio , Mandíbula/diagnóstico por imagem , Mandíbula/cirurgia , Impressão Tridimensional
10.
Sci Rep ; 13(1): 1093, 2023 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-36658170

RESUMO

Animal-robot interaction studies provide outstanding opportunities to understand the principles of social interactions. Here we investigated whether dogs' behaviour toward a cooperative artificial agent (Unidentified Moving Object (UMO)) is influenced by receiving a reward directly from the agent, and by variability in the UMO's location. In a problem-solving task, the UMO either helped dogs to obtain food (Direct Reward Group, DRG) or to fetch an object followed by an indirect reward from the owner/experimenter (Indirect Reward Group, IRG). During the Familiarization, the UMO either started from the same location or changed its starting location in all trials. In the Test phase, dogs faced the same task, but additionally a second, unfamiliar UMO was present. We found that both reward groups gazed at the UMO with decreasing latency during the Familiarization, with the IRG showing more gaze alternations between UMO and hiding location. Dogs showed no preference for either UMO in the Test phase but looked at the familiar UMO sooner if it had changed its location during the Familiarization. Thus, direct reward is not necessary to elicit elements of socially competent behavior in dogs, but variability in its motion may be important to improve the UMO's animacy and promote flexible learning.


Assuntos
Comportamento Animal , Comportamento Social , Animais , Cães , Resolução de Problemas , Aprendizagem , Recompensa
12.
Vet Rec ; 192(2): 53, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36661144
17.
Med. oral patol. oral cir. bucal (Internet) ; 28(1): e48-e55, ene. 2023. ilus, graf
Artigo em Espanhol | IBECS | ID: ibc-EMG-519

RESUMO

Background: Successful osseointegration of endosteal dental implants has been attributed to implant design, including the macro-, micro- and nano- geometric properties. Based on current literature pertaining to implant design, the resultant cellular and bone healing response is unknown when the thread thickness of the implants is increased, resulting in an increased contact area in implants designed with healing chambers. The aim of this study was to evaluate the effect of two implant designs with different thread profiles on the osseointegration parameters and implant stability at 3- and 6-weeks in vivo using a well-established preclinical dog model.Material and methods: A total of 48 type V Ti alloy implants were divided in two groups according to their thread design (D1= +0.1x/mm and D2= +0.15x/mm) and placed in an interpolated fashion into the radii of six beagles. Insertion torque was measured at time of placement, radii were extracted for histological processing following 3- and 6-week healing intervals. Histologic and histomorphometric analyses were performed in terms of bone to implant contact (%BIC) and bone area fraction occupancy within implant threads (%BAFO). Statistical analyses were performed through a linear mixed model with fixed factors of time and implant thread design.Results: Surface roughness analysis demonstrated no significant differences in Sa and Sq between D1 and D2 implant designs, which confirmed that both implant designs were homogenous except for their respective thread profiles. For insertion torque, statistically significant lower values were recorded for D1 in comparison to D2 (59.6 ± 11.1 and 78.9 ± 10.1 N⋅cm, respectively). Furthermore, there were no significant differences with respect to histological analysis and histomorphometric parameters, between D1 and D2 at both time points.(AU)


Assuntos
Animais , Cães , Osseointegração , Implantes Dentários , Implantação Dentária Endóssea/métodos , Propriedades de Superfície , Planejamento de Prótese Dentária , Torque
18.
In Vivo ; 37(1): 247-251, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36593007

RESUMO

BACKGROUND/AIM: Atlantoaxial subluxation (AAS) is a congenital or traumatic condition that often requires surgical stabilization. Surgery is performed via a ventral or dorsal approach. A ventral approach is challenging in toy breed dogs due to their small-sized bones. Reducing AAS by orthopedic wire via a dorsal approach can cause iatrogenic spinal cord damage. Due to these limitations, a Kishigami atlantoaxial tension band (Kishigami AATB) that remains in the epidural space has been devised. Similar to the Kishigami AATB, the present study developed a modified dorsal wiring method and evaluated it in toy breed dogs with AAS. MATERIALS AND METHODS: Medical data of toy breed dogs with AAS that underwent surgical stabilization using the modified dorsal wiring method from 2017 to 2020 were retrospectively reviewed. RESULTS: A total of 10 dogs were analyzed. Regarding the history of these dogs, six dogs had congenital AAS, and the remaining four dogs had traumatic AAS. Evaluation via computed tomography was available for five dogs, of which two dogs were identified as having incomplete ossification of their atlas. Although four dogs required a revision surgery because of recurrence of clinical signs or fracture of the atlas, final functional improvement was achieved in nine dogs. One dog showed worsened neurological status that led to death. CONCLUSION: Clinical results with the modified dorsal wiring method were similar to those with the Kishigami AATB. The modified dorsal wiring method is versatile as it could be applied to various shapes of dogs' atlas. Considering the shape of the atlas, it is recommended to apply the implant as far from the midline of the dorsal arch as possible to avoid fractures. With selection of suitable patients, this modified dorsal wiring method can be applied to dorsal stabilization of AAS in toy breed dogs.


Assuntos
Articulação Atlantoaxial , Doenças do Cão , Fraturas Ósseas , Luxações Articulares , Cães , Animais , Estudos Retrospectivos , Articulação Atlantoaxial/cirurgia , Doenças do Cão/cirurgia , Luxações Articulares/cirurgia , Luxações Articulares/veterinária , Fios Ortopédicos
19.
In Vivo ; 37(1): 252-261, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36593028

RESUMO

BACKGROUND/AIM: N1S1 rat models are commonly used in human medicine to study hepatocellular carcinoma (HCC). However, their use in veterinary medicine has not been reported. Thus, the aim of this study was to investigate whether the N1S1 rat models could be used to study canine HCC. MATERIALS AND METHODS: The animals were divided into four groups: normal rat, N1S1 rat, normal dog, and HCC dog. Liver tissues of all animals were evaluated for vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), platelet-derived growth factor receptor (PDGFR)-α, PDGFR-ß, and c-kit by immunohistochemistry. Slides of each factor were scored according to the percentage of stained tumor cells and intensity of the staining. RESULTS: Scores of VEGF and c-kit were high both in the tumor groups (the N1S1 rat and HCC dog groups) and the normal groups of dogs and rats. PDGFR-α was lower in the N1S1 rat group than that in the normal rat group (p=0.0042). It was also lower in the HCC dog group compared to the normal dog group (p=0.0008). PDGFR-ß was higher in the HCC dog group than that in the normal dog group (p=0.0023) but was not detectable in the rat groups. EGFR was not detectable in any group. CONCLUSION: Based on immunochemistry results, PDGFR-α and PDGFR-ß can be used as biomarkers of canine HCC. Because PDGFR-α showed consistency between rats and dogs, it can be used for studying canine HCC.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , Animais , Cães , Ratos , Biomarcadores , Carcinoma Hepatocelular/patologia , Carcinoma Hepatocelular/veterinária , Neoplasias Hepáticas/patologia , Neoplasias Hepáticas/veterinária , Proteínas Proto-Oncogênicas c-kit/metabolismo , Receptores Proteína Tirosina Quinases/metabolismo , Receptor alfa de Fator de Crescimento Derivado de Plaquetas/metabolismo , Receptor beta de Fator de Crescimento Derivado de Plaquetas/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo , Doenças do Cão/patologia
20.
Viruses ; 15(1)2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36680285

RESUMO

In human beings, there are five reported variants of concern of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2). However, in contrast to human beings, descriptions of infections of animals with specific variants are still rare. The aim of this study is to systematically investigate SARS-CoV-2 infections in companion animals in close contact with SARS-CoV-2-positive owners ("COVID-19 households") with a focus on the Delta variant. Samples, obtained from companion animals and their owners were analyzed using a real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) and next-generation sequencing (NGS). Animals were also tested for antibodies and neutralizing activity against SARS-CoV-2. Eleven cats and three dogs in nine COVID-19-positive households were RT-qPCR and/or serologically positive for the SARS-CoV-2 Delta variant. For seven animals, the genetic sequence could be determined. The animals were infected by one of the pangolin lineages B.1.617.2, AY.4, AY.43 and AY.129 and between zero and three single-nucleotide polymorphisms (SNPs) were detected between the viral genomes of animals and their owners, indicating within-household transmission between animal and owner and in multi-pet households also between the animals. NGS data identified SNPs that occur at a higher frequency in the viral sequences of companion animals than in viral sequences of humans, as well as SNPs, which were exclusively found in the animals investigated in the current study and not in their owners. In conclusion, our study is the first to describe the SARS-CoV-2 Delta variant transmission to animals in Switzerland and provides the first-ever description of Delta-variant pangolin lineages AY.129 and AY.4 in animals. Our results reinforce the need of a One Health approach in the monitoring of SARS-CoV-2 in animals.


Assuntos
COVID-19 , SARS-CoV-2 , Animais , Cães , Humanos , COVID-19/veterinária , Imunidade , Pangolins , Animais de Estimação , SARS-CoV-2/genética , Suíça/epidemiologia , Gatos
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