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J. Health Biol. Sci. (Online) ; 10(1): 1-9, 01/jan./2022. ilus, tab, graf
Artigo em Inglês | LILACS | ID: biblio-1378522


Objective: the aim of this study was to relate sociodemographic, epidemiological and clinical conditions to the occurrence of severe cases of HZ in reference hospital of Fortaleza. Methods: this is a cross-sectional analytical study, based on medical records of patients admitted from 2009 to 2018. Pearson's x2 test or Fisher's exact test were used when appropriate. Results: we analyzed 196 medical records. The presence of complications occurred in 69.9%, the most affected region was the cranial (68.9%), and 1.5% died. The presence of vesicles (PR=1.37; 95%CI: 1.03-1.82; p=0.01) and the choice of antibiotic associated antiviral therapy (PR=0.58; 95%CI: 0.46-0.73; p=0.00) were significantly associated with the severity. Conclusions: the disease may be more severe at ages over 50. The presence of lesions in vesicles was associated with a higher prevalence of complications and the use of antibiotics and antivirals as a protective factor.

Objetivo: relacionar condições sociodemográficas, epidemiológicas e clínicas à ocorrência de casos graves de HZ em hospital de referência de Fortaleza. Métodos: trata-se de um estudo analítico transversal, baseado em prontuários de pacientes internados de 2009 a 2018. Foram utilizados o teste x2 de Pearson ou o teste exato de Fisher, quando apropriado. Resultados: foram analisados 196 prontuários. A presença de complicações ocorreu em 69,9%, a região mais acometida foi a craniana (68,9%), e 1,5% foi a óbito. A presença de vesículas (RP=1,37; IC95%: 1,03-1,82; p=0,01) e a escolha da terapia antiviral associada a antibióticos (RP=0,58; IC95%: 0,46-0,73; p=0,00) foram significativamente associadas com a gravidade. Conclusões: a doença pode ser mais grave a partir dos 50 anos. A presença de lesões em vesículas foi associada à maior prevalência de complicações e o uso de antibióticos e antivirais como fator de proteção.

Herpes Zoster , Registros Médicos , Doença , Epidemiologia , Herpesvirus Humano 3 , Hospitalização , Pacientes Internados , Métodos
Recurso na Internet em Inglês, Espanhol, Português | LIS - Localizador de Informação em Saúde | ID: lis-49199


Estima-se que 10,6 milhões de pessoas ficaram doentes por tuberculose (TB) em 2021, um aumento de 4,5% em relação a 2020, e 1,6 milhão de pessoas morreram de TB (incluindo 187 mil entre as pessoas vivendo com HIV), de acordo com o relatório da Organização Mundial da Saúde (OMS) de 2022 sobre tuberculose global.

Tuberculose/mortalidade , Doença , COVID-19
Lima; Perú. Ministerio de Salud; Nov. 2022. 39 p. ilus.
Monografia em Espanhol | MINSAPERÚ | ID: biblio-1399992


El documento detalla los requisitos, condiciones y otras especificaciones necesarias para el procedimiento de reasignación bajo el régimen especial de contratación administrativa de servicios - CAS, tercera convocatoria, conforme a los numerales 27.7 y 27.8 del artículo 27 de la Ley N° 31538; así como; promover que las unidades ejecutoras del Ministerio de Salud, unidades ejecutoras del salud de los gobiernos regionales, Instituto Nacional de Salud e Instituto Nacional de Enfermedades Neoplásicas, conduzcan el procedimiento bajo los principios establecidos en el presente lineamiento

Gestão de Recursos Humanos , Saúde , Doença , Pessoal de Saúde , Contratos
Bioengineered ; 13(6): 14770-14779, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-36250718


miRNA is a small endogenous RNA and an important regulator of gene expression. miR-4443 is abnormally expressed in 12 diseases including cancer. The expression of miR-4443 is regulated by 3 upstream factors. miR-4443 has 12 downstream target genes. miR-4443 inhibits the expression of its target genes, thereby affecting the migration, proliferation, and invasion of pathological cells. miR-4443 participates in 4 signaling pathways and plays a role in the occurrence and development of several diseases. In addition, miR-4443 can also promote resistance to multiple drugs. Here, this article summarizes the aberrant expression of miR-4443 and its pathogenic molecular mechanisms in human diseases, which provides clues and directions for the follow-up research of miR-4443.

Dysregulation of miR-4443 affects the behaviors of diseased cells.miR-4443 is involved in the regulation of four signaling pathways.miR-4443 is associated with reduced drug sensitivity in cancer patients.

Doença , MicroRNAs , Humanos , MicroRNAs/genética , MicroRNAs/metabolismo , Doença/genética , Neoplasias/genética , Regulação da Expressão Gênica
Science ; 378(6616): eabm5874, 2022 10 14.
Artigo em Inglês | MEDLINE | ID: mdl-36227997


Cellular barcodes are distinct DNA sequences that enable one to track specific cells across time or space. Recent advances in our ability to detect natural or synthetic cellular barcodes, paired with single-cell readouts of cell state, have markedly increased our knowledge of clonal dynamics and genealogies of the cells that compose a variety of tissues and organs. These advances hold promise to redefine our view of human disease. Here, we provide an overview of cellular barcoding approaches, discuss applications to gain new insights into disease mechanisms, and provide an outlook on future applications. We discuss unanticipated insights gained through barcoding in studies of cancer and blood cell production and describe how barcoding can be applied to a growing array of medical fields, particularly with the increasing recognition of clonal contributions in human diseases.

Evolução Clonal , Código de Barras de DNA Taxonômico , Doença , Humanos , Evolução Clonal/genética , Doença/genética , Análise de Célula Única
Proc Natl Acad Sci U S A ; 119(41): e2209699119, 2022 10 11.
Artigo em Inglês | MEDLINE | ID: mdl-36191236


Fungi and bacteria often engage in complex interactions, such as the formation of multicellular biofilms within the human body. Knowledge about how interkingdom biofilms initiate and coalesce into higher-level communities and which functions the different species carry out during biofilm formation remain limited. We found native-state assemblages of Candida albicans (fungi) and Streptococcus mutans (bacteria) with highly structured arrangement in saliva from diseased patients with childhood tooth decay. Further analyses revealed that bacterial clusters are attached within a network of fungal yeasts, hyphae, and exopolysaccharides, which bind to surfaces as a preassembled cell group. The interkingdom assemblages exhibit emergent functions, including enhanced surface colonization and growth rate, stronger tolerance to antimicrobials, and improved shear resistance, compared to either species alone. Notably, we discovered that the interkingdom assemblages display a unique form of migratory spatial mobility that enables fast spreading of biofilms across surfaces and causes enhanced, more extensive tooth decay. Using mutants, selective inactivation of species, and selective matrix removal, we demonstrate that the enhanced stress resistance and surface mobility arise from the exopolymeric matrix and require the presence of both species in the assemblage. The mobility is directed by fungal filamentation as hyphae extend and contact the surface, lifting the assemblage with a "forward-leaping motion." Bacterial cell clusters can "hitchhike" on this mobile unit while continuously growing, to spread across the surface three-dimensionally and merge with other assemblages, promoting community expansion. Together, our results reveal an interkingdom assemblage in human saliva that behaves like a supraorganism, with disease-causing emergent functionalities that cannot be achieved without coassembly.

Biofilmes , Saliva , Streptococcus mutans , Candida albicans/metabolismo , Criança , Doença , Humanos , Hifas/fisiologia , Dinâmica Populacional , Saliva/microbiologia
Oxid Med Cell Longev ; 2022: 7657876, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36071866


The present review gathers together the most important information about variability in clusterin molecular structure, its profile, and the degree of glycosylation occurring in human tissues and body fluids in the context of the utility of these characteristics as potential diagnostic biomarkers of selected pathophysiological conditions. The carbohydrate part of clusterin plays a crucial role in many biological processes such as endocytosis and apoptosis. Many pathologies associated with neurodegeneration, carcinogenesis, metabolic diseases, and civilizational diseases (e.g., cardiovascular incidents and male infertility) have been described as causes of homeostasis disturbance, in which the glycan part of clusterin plays a very important role. The results of the discussed studies suggest that glycoproteomic analysis of clusterin may help differentiate the severity of hippocampal atrophy, detect the causes of infertility with an immune background, and monitor the development of cancer. Understanding the mechanism of clusterin (CLU) action and its binding epitopes may enable to indicate new therapeutic goals. The carbohydrate part of clusterin is considered necessary to maintain its proper molecular conformation, structural stability, and proper systemic and/or local biological activity. Taking into account the wide spectrum of CLU action and its participation in many processes in the human body, further studies on clusterin glycosylation variability are needed to better understand the molecular mechanisms of many pathophysiological conditions. They can also provide the opportunity to find new biomarkers and enrich the panel of diagnostic parameters for diseases that still pose a challenge for modern medicine.

Clusterina , Doença , Glicosilação , Biomarcadores/metabolismo , Clusterina/metabolismo , Humanos , Polissacarídeos
Proc Natl Acad Sci U S A ; 119(37): e2204206119, 2022 09 13.
Artigo em Inglês | MEDLINE | ID: mdl-36067306


In natural populations, the same mutation can lead to different phenotypic outcomes due to the genetic variation that exists among individuals. Such genetic background effects are commonly observed, including in the context of many human diseases. However, systematic characterization of these effects at the species level is still lacking to date. Here, we sought to comprehensively survey background-dependent traits associated with gene loss-of-function (LoF) mutations in 39 natural isolates of Saccharomyces cerevisiae using a transposon saturation strategy. By analyzing the modeled fitness variability of a total of 4,469 genes, we found that 15% of them, when impacted by a LoF mutation, exhibited a significant gain- or loss-of-fitness phenotype in certain natural isolates compared with the reference strain S288C. Out of these 632 genes with predicted background-dependent fitness effects, around 2/3 impact multiple backgrounds with a gradient of predicted fitness change while 1/3 are specific to a single genetic background. Genes related to mitochondrial function are significantly overrepresented in the set of genes showing a continuous variation and display a potential functional rewiring with other genes involved in transcription and chromatin remodeling as well as in nuclear-cytoplasmic transport. Such rewiring effects are likely modulated by both the genetic background and the environment. While background-specific cases are rare and span diverse cellular processes, they can be functionally related at the individual level. All genes with background-dependent fitness effects tend to have an intermediate connectivity in the global genetic interaction network and have shown relaxed selection pressure at the population level, highlighting their potential evolutionary characteristics.

Aptidão Genética , Mutação com Perda de Função , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae , Doença/genética , Redes Reguladoras de Genes , Humanos , Fenótipo , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/genética
Lima; Perú. Ministerio de Salud; Set. 2022. 5 p.
Monografia em Espanhol | MINSAPERÚ | ID: biblio-1395511


La norma técnica de salud contiene los proceso, metodología e instrumentos para la vigilancia epidemiológica de las Enfermedades Diarreicas Agudas (EDA) en el país.

Doença , Metodologia como Assunto , Monitoramento Epidemiológico , Métodos , Disenteria
Science ; 377(6610): 1077-1085, 2022 09 02.
Artigo em Inglês | MEDLINE | ID: mdl-35951677


Mammalian genomes have multiple enhancers spanning an ultralong distance (>megabases) to modulate important genes, but it is unclear how these enhancers coordinate to achieve this task. We combine multiplexed CRISPRi screening with machine learning to define quantitative enhancer-enhancer interactions. We find that the ultralong distance enhancer network has a nested multilayer architecture that confers functional robustness of gene expression. Experimental characterization reveals that enhancer epistasis is maintained by three-dimensional chromosomal interactions and BRD4 condensation. Machine learning prediction of synergistic enhancers provides an effective strategy to identify noncoding variant pairs associated with pathogenic genes in diseases beyond genome-wide association studies analysis. Our work unveils nested epistasis enhancer networks, which can better explain enhancer functions within cells and in diseases.

Doença , Elementos Facilitadores Genéticos , Epistasia Genética , Aprendizado de Máquina , Proteínas de Ciclo Celular , Doença/genética , Estudo de Associação Genômica Ampla , Humanos , Células K562 , Proteínas Nucleares/genética , Fatores de Transcrição/genética
Lima; Perú. Ministerio de Salud; Ago. 2022. 24 p. ilus.
Monografia em Espanhol | MINSAPERÚ | ID: biblio-1395490


El documento contiene la estrategia para contribuir en la reducción de la morbilidad, discapacidad y mortalidad causada por las enfermedades inmunoprevenibles

Criança , Doença , Morbidade , Mortalidade , Vacinação
Rev. med. vet. zoot ; 69(2): 155-165, mayo-ago. 2022. tab, graf
Artigo em Espanhol | LILACS, COLNAL | ID: biblio-1395018


Resumen Los virus de inmunodeficiencia y leucemia felina representan un problema de gran envergadura para los felinos domésticos debido a la multiplicidad de sintomatologías que manifiestan. El objetivo del presente estudio fue establecer, retrospectivamente, la prevalencia en la presentación de ViLeF y VIF en pacientes de seis clínicas de pequeños animales en Bogotá y Chía, en relación con factores como su edad, raza y género. Se realizó un estudio transversal y retrospectivo, mediante la recopilación de datos de 1.014 historias clínicas de pacientes felinos que ingresaron a seis clínicas de la ciudad de Bogotá y Chía, para determinar la prevalencia de VIF y ViLeF y la asociación de estas con factores como edad, género y raza, entre 2015 y 2019, a través de la prueba OR. La detección de los virus se realizó mediante una prueba rápida basada en inmunocromatografía. La mayor prevalencia para cada enfermedad por año fue: 12,3% para VIF en 2012 y 18% para ViLeF en 2019. Los machos presentaron mayores seroprevalencias para ambas enfermedades durante la mayoría los años evaluados. Factores como raza (criolla: VIF: 1,85; ViLeF: 2,01), género (macho: VIF: 1,53 OR; ViLeF: 1,64) y edad (> 7 años: VIF: 3,82; ViLeF: 3,21) se relacionaron positivamente con la presentación de ambas enfermedades en la población felina evaluada.

Abstract Immunodeficiency virus and feline leukemia virus represent major problems for domestic felines due to the multiplicity of symptoms they manifest. The objective of the present study was to establish, retrospectively, the prevalence in the presentation of FeLV and FIV in patients from six small animal clinics in Bogota and Chia, related to factors such as age, race, and gender. A cross-sectional and retrospective study was carried out, collecting data from 1.014 clinical records of feline patients who were admitted to six clinics in the city of Bogota and Chia, to determine the prevalence of FIV and FeLV and their association with factors such as age, gender, and race, between 2015 and 2019 through the OR test. The detection of the viruses was carried out through a rapid test based on immunochromatography. The highest prevalence for each disease per year was 12,3% for FIV in 2012 and 18% for FeLV in 2019. Males presented higher seroprevalences for both diseases during most of the years evaluated. Factors such as race (Creole: FIV: 1,85; FeLV: 2,01), gender (male: FIV: 1.53 OR, FeLV: 1,64), and age (> 7 years: FIV: 3.82; FeLV: 3.21) were positively related to the presentation of both diseases in the feline population evaluated.

Animais , Gatos , Vírus , Ensaio de Imunoadsorção Enzimática , Leucemia , Doença Crônica , Doença , Cromatografia de Afinidade , Vírus da Imunodeficiência Felina , Vírus da Leucemia Felina , Diagnóstico , Retroviridae , Hospitais Veterinários
Front Immunol ; 13: 891220, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35967383


Ym1 is a rodent-specific chitinase-like protein (CLP) lacking catalytic activity, whose cellular origins are mainly macrophages, neutrophils and other cells. Although the detailed function of Ym1 remains poorly understood, Ym1 has been generally recognized as a fundamental feature of alternative activation of macrophages in mice and hence one of the prevalent detecting targets in macrophage phenotype distinguishment. Studies have pointed out that Ym1 may have regulatory effects, which are multifaceted and even contradictory, far more than just a mere marker. Allergic lung inflammation, parasite infection, autoimmune diseases, and central nervous system diseases have been found associations with Ym1 to varying degrees. Thus, insights into Ym1's role in diseases would help us understand the pathogenesis of different diseases and clarify the genuine roles of CLPs in mammals. This review summarizes the information on Ym1 from the gene to its expression and regulation and focuses on the association between Ym1 and diseases.

Doença , Lectinas , Macrófagos , beta-N-Acetil-Hexosaminidases , Animais , Quitinases/genética , Quitinases/imunologia , Doença/genética , Imunidade/genética , Imunidade/imunologia , Lectinas/genética , Lectinas/imunologia , Macrófagos/imunologia , Mamíferos/genética , Mamíferos/imunologia , Camundongos , Neutrófilos/imunologia , beta-N-Acetil-Hexosaminidases/genética , beta-N-Acetil-Hexosaminidases/imunologia
Int J Mol Sci ; 23(16)2022 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-36012629


The increasing population will challenge healthcare, particularly because the worldwide population has never been older. Therapeutic solutions to age-related disease will be increasingly critical. Kinases are key regulators of human health and represent promising therapeutic targets for novel drug candidates. The dual-specificity tyrosine-regulated kinase (DYRKs) family is of particular interest and, among them, DYRK1A has been implicated ubiquitously in varied human diseases. Herein, we focus on the characteristics of DYRK1A, its regulation and functional role in different human diseases, which leads us to an overview of future research on this protein of promising therapeutic potential.

Proteínas Serina-Treonina Quinases , Proteínas Tirosina Quinases , Doença , Humanos , Proteínas Serina-Treonina Quinases/genética , Proteínas Tirosina Quinases/genética , Proteínas Tirosina Quinases/metabolismo
Univ. salud ; 24(2): 170-183, mayo-ago. 2022. tab, graf
Artigo em Espanhol | LILACS, COLNAL | ID: biblio-1377465


Introducción: La prediabetes es un estado intermedio de hiperglicemia, con una alta prevalencia en la población colombiana. Su relación con enfermedad cardiovascular y complicaciones macro y microvasculares se ha establecido, incrementando la evidencia científica en la literatura reciente. Objetivo: Describir las enfermedades cardiovasculares (complicaciones macrovasculares) relacionadas con prediabetes, su diagnóstico, fisiopatología, el enfoque de manejo y el tratamiento avalado para prevenir estas complicaciones. Materiales y métodos: Se realizó una revisión narrativa de la literatura, mediante búsqueda de artículos científicos en Medline entre 2015 y 2021. Resultados: Se seleccionaron 83 artículos. Se describe la relación de prediabetes con enfermedad cardiovascular, mortalidad, diagnóstico y tratamiento conforme a la literatura actualizada. Conclusiones: La importancia de la prediabetes radica en su asociación con complicaciones macrovasculares y su mortalidad, lo que pone en evidencia la importancia de estudiarla, seguirla y tratarla para evitar la progresión a diabetes mellitus, el desarrollo de desenlaces que finalizan en aumento de la morbimortalidad, alteración de la calidad de vida e incremento de los costos en salud.

Introduction: Prediabetes is an intermediate state of hyperglycemia with a high prevalence in the Colombian population. The relationship between prediabetes and cardiovascular disease as well as its association with macro and microvascular complications has been established, with scientific evidence increasing in recent literature. Objective: To describe cardiovascular diseases (macrovascular complications) related to prediabetes, their diagnosis, physiopathology, management approach, and treatment to prevent those complications. Materials and methods: A narrative review of the literature was conducted, searching for scientific articles in Medline during the 2015-2021 period. Results: 83 articles were selected, which described the relationship between prediabetes with cardiovascular disease, mortality, diagnosis, and treatment based on recent reports. Conclusions: The importance of prediabetes lies in its association with macrovascular complications and its mortality. This association highlights the importance of studying, monitoring, and treating this disease to avoid its progression to diabetes mellitus, the development of outcomes that end in increased morbidity and mortality, diminished quality of life, and increased health care costs.

Humanos , Qualidade de Vida , Doença , Estado Pré-Diabético , Doenças Cardiovasculares , Morbidade , Diabetes Mellitus , Diagnóstico
Methods Mol Biol ; 2537: 1-19, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35895255


Alternative pre-mRNA splicing allows for the production of multiple mRNAs from an individual gene, which not only expands the protein-coding potential of the genome but also enables complex mechanisms for the post-transcriptional control of gene expression. Regulation of alternative splicing entails a combinatorial interplay between an abundance of trans-acting splicing factors, cis-acting regulatory sequence elements and their concerted effects on the core splicing machinery. Given the extent and biological significance of alternative splicing in humans, it is not surprising that aberrant splicing patterns can cause or contribute to a wide range of diseases. In this introductory chapter, we outline the mechanisms that govern alternative pre-mRNA splicing and its regulation and discuss how dysregulated splicing contributes to human diseases affecting the motor system and the brain.

Processamento Alternativo , Precursores de RNA , Doença/genética , Humanos , Precursores de RNA/genética , Precursores de RNA/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Sequências Reguladoras de Ácido Nucleico , Transativadores/metabolismo
RECIIS (Online) ; 16(2): 488-495, abr.-jun. 2022. ilus
Artigo em Português | LILACS | ID: biblio-1378893


Esta resenha aborda o filme Contágio (Contagion), longa-metragem norte-americano de 2011, com direção de Steven Soderbergh e roteiro de Scott Z. Burns. O filme acompanha a trajetória de disseminação de um vírus zoonótico a partir de Hong Kong para o resto do mundo. Enquanto isso, autoridades científicas e sanitárias se mobilizam para combater a nova doença, que rapidamente atinge diversos países, fazendo milhares de vítimas. Em 2020, a procura pelo thriller aumentou devido à semelhança com a pandemia de covid-19, evidenciando o potencial do cinema para a divulgação científica ao abordar conceitos, processos e controvérsias da ciência. Com um roteiro cientificamente apurado, Contágio também ressalta o potencial preditivo da ciência em relação a emergências sanitárias.

This review addresses the 2011 American feature film Contagion, directed by Steven Soderbergh and screenplayed by Scott Z. Burns. The film follows the trajectory of the spread of a zoonotic virus from Hong Kong to the rest of the world. Meanwhile, scientific and health authorities are mobilizing to fight the new disease, which is rapidly reaching several countries, claiming thousands of victims. In 2020, the demand for the thriller increased due to the similarity with the covid-19 pandemic, highlighting the potential of cinema for science communication by approaching science's concepts, processes and controversies. With a scientifically accurate script, Contagion also underlines the predictive potential of science in relation to health emergencies.

Esta reseña aborda el largometraje estadounidense de 2011 Contagio (Contagion), dirigido por Steven Soderbergh y con guión de Scott Z. Burns. La película sigue la trayectoria de la propagación de un virus zoonótico desde Hong Kong al resto del mundo. Mientras tanto, las autoridades científicas y sanitarias se movilizan para combatir la nueva enfermedad, que rápidamente llega a varios países y se cobra miles de víctimas. En 2020 aumentó la demanda del thriller por la similitud con la pandemia del covid-19, destacando el potencial del cine para la divulgación científica al abordar conceptos, procesos y controversias de la ciencia. Con un guión científicamente certero, Contagio también destaca el potencial predictivo de la ciencia en relación con las emergencias sanitarias.

Humanos , Vírus , Transmissão de Doença Infecciosa , Comunicação e Divulgação Científica , Filmes Cinematográficos , Doença , Obra de Ficção , Pandemias , COVID-19