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1.
PLoS One ; 19(6): e0302578, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38829861

RESUMO

Particle-in-cell (PIC) simulation serves as a widely employed method for investigating plasma, a prevalent state of matter in the universe. This simulation approach is instrumental in exploring characteristics such as particle acceleration by turbulence and fluid, as well as delving into the properties of plasma at both the kinetic scale and macroscopic processes. However, the simulation itself imposes a significant computational burden. This research proposes a novel implementation approach to address the computationally intensive phase of the electrostatic PIC simulation, specifically the Particle-to-Interpolation phase. This is achieved by utilizing a high-speed Field Programmable Gate Array (FPGA) computation platform. The suggested approach incorporates various optimization techniques and diminishes memory access latency by leveraging the flexibility and performance attributes of the Intel FPGA device. The results obtained from our study highlight the effectiveness of the proposed design, showcasing the capability to execute hundreds of functional operations in each clock cycle. This stands in contrast to the limited operations performed in a general-purpose single-core computation platform (CPU). The suggested hardware approach is also scalable and can be deployed on more advanced FPGAs with higher capabilities, resulting in a significant improvement in performance.


Assuntos
Simulação por Computador , Eletricidade Estática , Gases em Plasma
2.
Clin Oral Investig ; 28(6): 355, 2024 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-38833072

RESUMO

OBJECTIVES: Clinical trials testing new devices require prior training on dummies to minimize the "learning curve" for patients. Dentists were trained using a novel water jet device for mechanical cleaning of dental implants and with a novel cold plasma device for surface functionalisation during a simulated open flap peri-implantitis therapy. The hypothesis was that there would be a learning curve for both devices. MATERIALS AND METHODS: 11 dentists instrumented 44 implants in a dummy-fixed jaw model. The effect of the water jet treatment was assessed as stain removal and the effect of cold plasma treatment as surface wettability. Both results were analysed using photographs. To improve treatment skills, each dentist treated four implants and checked the results immediately after the treatment as feedback. RESULTS: Water jet treatment significantly improved from the first to the second implant from 62.7% to 75.3% stain removal, with no further improvement up to the fourth implant. The wettability with cold plasma application reached immediately a high level at the first implant and was unchanged to the 4th implant (mean scores 2.7 out of 3). CONCLUSION: A moderate learning curve was found for handling of the water jet but none for handling of the cold plasma. CLINICAL RELEVANCE: Scientific rational for study: Two new devices were developed for peri-implantitis treatment (Dental water jet, cold plasma). Dentists were trained in the use of these devices prior to the trial to minimize learning effects. PRINCIPAL FINDINGS: Experienced dentists learn the handling of the water jet very rapidly and for cold plasma they do not need much training. PRACTICAL IMPLICATIONS: A clinical study is in process. When the planned clinical study will be finished, we will find out, if this dummy head exercise really minimised the learning curve for these devices.


Assuntos
Descontaminação , Implantes Dentários , Gases em Plasma , Água , Humanos , Descontaminação/métodos , Peri-Implantite/prevenção & controle , Propriedades de Superfície , Molhabilidade
3.
Int J Mol Sci ; 25(11)2024 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-38892174

RESUMO

Foodborne diseases can be attributed not only to contamination with bacterial or fungal pathogens but also their associated toxins. Thus, to maintain food safety, innovative decontamination techniques for toxins are required. We previously demonstrated that an atmospheric-pressure dielectric-barrier discharge (APDBD) plasma generated by a roller conveyer plasma device is effective at inactivating bacteria and fungi in foods. Here, we have further examined whether the roller conveyer plasma device can be used to degrade toxins produced by foodborne bacterial pathogens, including aflatoxin, Shiga toxins (Stx1 and Stx2), enterotoxin B and cereulide. Each toxin was spotted onto an aluminum plate, allowed to dry, and then treated with APDBD plasma applied by the roller conveyer plasma device for different time periods. Assessments were conducted using a competitive enzyme-linked immunosorbent assay (ELISA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The results demonstrate a significant time-dependent decrease in the levels of these toxins. ELISA showed that aflatoxin B1 concentrations were reduced from 308.6 µg/mL to 74.4 µg/mL within 1 min. For Shiga toxins, Stx1 decreased from 913.8 µg/mL to 65.1 µg/mL, and Stx2 from 2309.0 µg/mL to 187.6 µg/mL within the same time frame (1 min). Enterotoxin B levels dropped from 62.67 µg/mL to 1.74 µg/mL at 15 min, and 1.43 µg/mL at 30 min, but did not display a significant decrease within 5 min. LC-MS/MS analysis verified that cereulide was reduced to below the detection limit following 30 min of APDBD plasma treatment. Taken together, these findings highlight that a range of foodborne toxins can be degraded by a relatively short exposure to plasma generated by an APDBD using a roller conveyer device. This technology offers promising advancements in food safety, providing a novel method to alleviate toxin contamination in the food processing industry.


Assuntos
Pressão Atmosférica , Espectrometria de Massas em Tandem , Enterotoxinas , Depsipeptídeos/química , Microbiologia de Alimentos/métodos , Cromatografia Líquida/métodos , Doenças Transmitidas por Alimentos/prevenção & controle , Doenças Transmitidas por Alimentos/microbiologia , Ensaio de Imunoadsorção Enzimática , Contaminação de Alimentos/análise , Gases em Plasma/química , Aflatoxina B1
4.
Int J Mol Sci ; 25(11)2024 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-38892350

RESUMO

Periodontitis is an inflammatory disease caused by Porphyromonas gingivalis (P. gingivalis) in the oral cavity. This periodontal disease causes damage to the periodontal ligament and alveolar bone and can cause tooth loss, but there is no definite treatment yet. In this study, we investigated the possibility of using no-ozone cold plasma to safely treat periodontitis in the oral cavity. First, human gingival fibroblasts (HGFs) were treated with P. gingivalis-derived lipopolysaccharide (PG-LPS) to induce an inflammatory response, and then the anti-inflammatory effect of NCP was examined, and a study was conducted to identify the mechanism of action. Additionally, the anti-inflammatory effect of NCP was verified in rats that developed an inflammatory response similar to periodontitis. When NCP was applied to PG-LPS-treated HGFs, the activities of inflammatory proteins and cytokines were effectively inhibited. It was confirmed that the process of denaturing the medium by charged particles of NCP is essential for the anti-inflammatory effect of NCP. Also, it was confirmed that repeated treatment of periodontitis rats with NCP effectively reduced the inflammatory cells and osteoclast activity. As a result, this study suggests that NCP can be directly helpful in the treatment of periodontitis in the future.


Assuntos
Anti-Inflamatórios , Fibroblastos , Gengiva , Lipopolissacarídeos , Periodontite , Porphyromonas gingivalis , Animais , Periodontite/microbiologia , Periodontite/tratamento farmacológico , Ratos , Anti-Inflamatórios/farmacologia , Humanos , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Ozônio/farmacologia , Gases em Plasma/farmacologia , Gases em Plasma/uso terapêutico , Masculino , Citocinas/metabolismo , Modelos Animais de Doenças , Óxido Nítrico/metabolismo , Células Cultivadas
5.
RNA Biol ; 21(1): 31-44, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-38828710

RESUMO

Non-thermal plasma, a partially ionized gas, holds significant potential for clinical applications, including wound-healing support, oral therapies, and anti-tumour treatments. While its applications showed promising outcomes, the underlying molecular mechanisms remain incompletely understood. We thus apply non-thermal plasma to mouse auricular skin and conducted non-coding RNA sequencing, as well as single-cell blood sequencing. In a time-series analysis (five timepoints spanning 2 hours), we compare the expression of microRNAs in the plasma-treated left ears to the unexposed right ears of the same mice as well as to the ears of unexposed control mice. Our findings indicate specific effects in the treated ears for a set of five miRNAs: mmu-miR-144-5p, mmu-miR-144-3p, mmu-miR-142a-5p, mmu-miR-223-3p, and mmu-miR-451a. Interestingly, mmu-miR-223-3p also exhibits an increase over time in the right non-treated ear of the exposed mice, suggesting systemic effects. Notably, this miRNA, along with mmu-miR-142a-5p and mmu-miR-144-3p, regulates genes and pathways associated with wound healing and tissue regeneration (namely ErbB, FoxO, Hippo, and PI3K-Akt signalling). This co-regulation is particularly remarkable considering the significant seed dissimilarities among the miRNAs. Finally, single-cell sequencing of PBMCs reveals the downregulation of 12 from 15 target genes in B-cells, Cd4+ and Cd8+ T-cells. Collectively, our data provide evidence for a systemic effect of non-thermal plasma.


Assuntos
Regulação da Expressão Gênica , MicroRNAs , Gases em Plasma , Pele , MicroRNAs/genética , Animais , Camundongos , Pele/metabolismo , Gases em Plasma/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Perfilação da Expressão Gênica , Cicatrização/efeitos dos fármacos , Transdução de Sinais , Sistema Imunitário/metabolismo
6.
Chemosphere ; 361: 142570, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38852636

RESUMO

Various contaminants of emerging concern (CECs) including pharmaceuticals and personal care products (PPCPs) have been known to threaten the aquatic ecosystem and human health even at low levels in surface water. Among them, the wide variety use of parabens as preservatives may pose potential threat to human because parabens may present estrogenic activity. Various advanced oxidation processes have been attempted to reduce parabens, but challenges using cold plasma (CP) are very rare. CP is worth paying attention to in reducing parabens because it has the advantage of generating radical ions, including reactive oxygen/nitrogen species and various ions. Accordingly, this study demonstrates how CP can be utilized and how CP competes with other advanced oxidation processes in energy requirements. Quantified ethyl-, propyl-, and butyl-paraben indicate that CP can effectively degrade them up to 99.1% within 3 h. Regression reveals that the kinetic coefficients of degradation can be increased to as high as 0.0328 min-1, comparable to other advanced oxidation processes. Many by-products generated from the oxidation of parabens provide evidence of the potential degradation pathway through CP treatment. In addition, we found that the electrical energy consumption per order of CP (39-95 kWh/m3/order) is superior to other advanced oxidation processes (69∼31,716 kWh/m3/order). Overall, these results suggest that CP may be a viable option to prevent adverse health-related consequences associated with parabens in receiving water.


Assuntos
Oxirredução , Parabenos , Poluentes Químicos da Água , Parabenos/química , Poluentes Químicos da Água/química , Gases em Plasma/química , Cinética , Conservantes Farmacêuticos/química
7.
Carbohydr Polym ; 339: 122288, 2024 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-38823936

RESUMO

This paper reports on biofunctionalisation of a poly(lactic acid) (PLA) film by surface activation through cold plasma treatment followed by coating with a chitosan-gelatin xerogel. The UV cross-linking of the xerogel precursor was simultaneously performed with the fixation onto the PLA support. This has a strong effect on surface properties, in terms of wettability, surface free energy, morphology and micromechanical features. The hydrophilic - hydrophobic character of the surface, determined by contact angle measurements, was tuned along the process, passing from moderate hydrophobic PLA to enhanced hydrophilic plasma activated surface, which favors coating adhesion, then to moderate hydrophobic chitosan-gelatin coating. The coating has a Lewis amphoteric surface, with a porous xerogel-like morphology, as revealed by scanning electron microscopy images. By riboflavin mediated UV cross-linking the chitosan-gelatin coating becomes high adhesive and with a more pronounced plasticity, as shown by AFM force-distance spectroscopy. Thus prepared surface-coated PLA supports were successfully tested for growth of dermal fibroblasts, which are known for their induction potential of chondrogenic cells, which is very important in cartilage tissue engineering.


Assuntos
Quitosana , Fibroblastos , Gelatina , Poliésteres , Quitosana/química , Gelatina/química , Poliésteres/química , Fibroblastos/efeitos dos fármacos , Fibroblastos/citologia , Humanos , Propriedades de Superfície , Géis/química , Raios Ultravioleta , Gases em Plasma/química , Interações Hidrofóbicas e Hidrofílicas , Materiais Revestidos Biocompatíveis/química , Reagentes de Ligações Cruzadas/química , Molhabilidade
8.
Environ Res ; 252(Pt 3): 119015, 2024 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-38692423

RESUMO

Carbon material modification and defect engineering are indispensable for bolstering the photocatalytic effectiveness of bismuth halide oxide (BiOX). In this study, a novel porous and defect-rich Ar-CB-2 photocatalyst was synthesized for emerging pollutants degradation. Leveraging the interfacial coupling effect of multi-walled carbon nanotubes (MWCNTs), we expanded the absorption spectrum of BiOI nanosheets and significantly suppressed the recombination of charge carriers. Introducing defects via Argon (Ar) plasma-etching further bolstered the adsorption efficacy and electron transfer properties of photocatalyst. In comparison to the pristine BiOI and CB-2, the Ar-CB-2 photocatalyst demonstrated superior photodegradation efficiency, with the first-order reaction rates for the photodegradation of tetracycline (TC) and bisphenol A (BPA) increasing by 2.83 and 4.53 times, respectively. Further probe experiments revealed that the steady-state concentrations of ·O2- and 1O2 in the Ar-CB-2/light system were enhanced by a factor of 1.67 and 1.28 compared to CB-2/light system. This result confirmed that the porous and defect-rich structure of Ar-CB-2 inhibited electron-hole recombination and boosted photocatalyst-oxygen interaction, swiftly transforming O2 into active oxygen species, thus accelerating their production. Furthermore, the possible degradation pathways for TC and BPA in the Ar-CB-2/light system were predicted. Overall, these findings offered a groundbreaking approach to the development of highly effective photocatalysts, capable of swiftly breaking down emerging pollutants.


Assuntos
Argônio , Compostos Benzidrílicos , Bismuto , Nanotubos de Carbono , Fenóis , Fotólise , Bismuto/química , Nanotubos de Carbono/química , Catálise , Porosidade , Fenóis/química , Compostos Benzidrílicos/química , Argônio/química , Tetraciclina/química , Poluentes Químicos da Água/química , Poluentes Ambientais/química , Processos Fotoquímicos , Gases em Plasma/química
9.
Pathog Dis ; 822024 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-38730561

RESUMO

Antibiotic resistance (ATBR) is increasing every year as the overuse of antibiotics (ATBs) and the lack of newly emerging antimicrobial agents lead to an efficient pathogen escape from ATBs action. This trend is alarming and the World Health Organization warned in 2021 that ATBR could become the leading cause of death worldwide by 2050. The development of novel ATBs is not fast enough considering the situation, and alternative strategies are therefore urgently required. One such alternative may be the use of non-thermal plasma (NTP), a well-established antimicrobial agent actively used in a growing number of medical fields. Despite its efficiency, NTP alone is not always sufficient to completely eliminate pathogens. However, NTP combined with ATBs is more potent and evidence has been emerging over the last few years proving this is a robust and highly effective strategy to fight resistant pathogens. This minireview summarizes experimental research addressing the potential of the NTP-ATBs combination, particularly for inhibiting planktonic and biofilm growth and treating infections in mouse models caused by methicillin-resistant Staphylococcus aureus or Pseudomonas aeruginosa. The published studies highlight this combination as a promising solution to emerging ATBR, and further research is therefore highly desirable.


Assuntos
Antibacterianos , Biofilmes , Gases em Plasma , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Gases em Plasma/farmacologia , Animais , Humanos , Biofilmes/efeitos dos fármacos , Pseudomonas aeruginosa/efeitos dos fármacos , Camundongos , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Farmacorresistência Bacteriana , Resistência Microbiana a Medicamentos , Infecções por Pseudomonas/microbiologia , Infecções por Pseudomonas/tratamento farmacológico , Modelos Animais de Doenças , Infecções Estafilocócicas/microbiologia , Infecções Estafilocócicas/tratamento farmacológico
10.
Food Res Int ; 187: 114418, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38763668

RESUMO

Interest in exploring alternative starch sources like finger millet is rising due to wide starch applications. However, native starch often lacks desired qualities, including rheological properties. Modification is thus necessary for specific end uses. Plasma treatment as a greener and sustainable method for starch modification was therefore, studied for its ability to impact rheological properties of finger millet starch (FMS). Considerable changes in the rheological properties on FMS was noted, a significant decrease and increase (p < 0.05) in the peak viscosity (from 3.35 to 0.553 Pa.s) and paste clarity respectively was observed, indicating occurrence of depolymerization. However, intermediate plasma-treated samples (200 V) observed a decrease in paste clarity attributed to aggregate formation and cross-linking. Cross-linking was also confirmed by findings of frequency sweep where a continuous decrease in G' values of plasma treated FMS gel was interrupted by sudden increase. Despite depolymerization causing alteration of rheological behaviour such as decrease in shear thinning properties, gel strength observed a contradictory increase. This was attributed to incorporation of functional group and absence of shear responsible for network formation giving higher gel strength to FMS gels. This is elaborated in detail in the study. The study thus concluded that cold plasma significantly impacted all the rheological properties of the FMS and hence can prove to be beneficial for modification of starch rheological parameters.


Assuntos
Eleusine , Géis , Gases em Plasma , Reologia , Amido , Amido/química , Gases em Plasma/química , Viscosidade , Eleusine/química , Géis/química , Pressão Atmosférica , Manipulação de Alimentos/métodos
11.
Chemosphere ; 358: 142211, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38697573

RESUMO

This paper investigates the effects of argon (Ar) and that of Ar mixed with ambient air (Ar-Air) cold plasma jets (CPJs) on 4-nitrophenol (4-NP) degradation using low input power. The introduction of ambient air into the Ar-Air plasma jet enhances ionization-driven processes during high-voltage discharge by utilizing nitrogen and oxygen molecules from ambient air, resulting in increased reactive oxygen and nitrogen species (RONS) production, which synergistically interacts with argon. This substantial generation of RONS establishes Ar-Air plasma jet as an effective method for treating 4-NP contamination in deionized water (DW). Notably, the Ar-Air plasma jet treatment outperforms that of the Ar jet. It achieves a higher degradation rate of 97.2% and a maximum energy efficiency of 57.3 gkW-1h-1, following a 6-min (min) treatment with 100 mgL-1 4-NP in DW. In contrast, Ar jet treatment yielded a lower degradation rate and an energy efficiency of 75.6% and 47.8 gkW-1h-1, respectively, under identical conditions. Furthermore, the first-order rate coefficient for 4-NP degradation was measured at 0.23 min-1 for the Ar plasma jet and significantly higher at 0.56 min-1 for the Ar-Air plasma jet. Reactive oxygen species, such as hydroxyl radical and ozone, along with energy from excited species and plasma-generated electron transfers, are responsible for CPJ-assisted 4-NP breakdown. In summary, this study examines RONS production from Ar and Ar-Air plasma jets, evaluates their 4-NP removal efficacy, and investigates the biocompatibility of 4-NP that has been degraded after plasma treatment.


Assuntos
Argônio , Nitrofenóis , Gases em Plasma , Nitrofenóis/química , Argônio/química , Gases em Plasma/química , Ar , Espécies Reativas de Oxigênio/metabolismo , Poluentes Químicos da Água/toxicidade
12.
Ophthalmic Plast Reconstr Surg ; 40(3): e89-e91, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38738721

RESUMO

A 40-year-old woman underwent periocular plasma skin regeneration, a cosmetic treatment for periorbital rejuvenation. She subsequently developed bilateral thermal keratitis, manifesting as blurred vision, irritation, and redness, with a vision decrease to 20/60 and 20/50 in her OD and OS, respectively. Examination demonstrated bilateral large, irregular corneal epithelial defects and edema, necessitating treatment with amniotic membrane grafts, bandage contact lenses, and hypertonic saline. One year posttreatment, her visual acuity improved to 20/20 and 20/25, albeit with ongoing symptomatic dryness and bilateral anterior stromal haze. This case, as only the second reported instance of ocular damage from periocular plasma skin regeneration, underscores the need for heightened awareness of potential ocular complications following plasma skin regeneration and reinforces the importance of protective measures during periocular procedures.


Assuntos
Queimaduras Oculares , Humanos , Feminino , Adulto , Queimaduras Oculares/induzido quimicamente , Queimaduras Oculares/diagnóstico , Ceratite/diagnóstico , Ceratite/etiologia , Ceratite/fisiopatologia , Gases em Plasma/uso terapêutico , Regeneração/fisiologia , Técnicas Cosméticas/efeitos adversos , Acuidade Visual
13.
BMC Plant Biol ; 24(1): 420, 2024 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-38760701

RESUMO

Cold atmospheric plasma (CAP) is a physical technology with notable effects on living organisms. In the present study, tomato seeds (Solanum lycopersicum var. Bassimo Mill.) were exposed to CAP for various time intervals, ranging from 1 to 5 min, in both continuous and intermittent periods, and were compared with a control group that received no CAP treatment. Seedlings grown from treated seeds exhibited improvements in levels of growth traits, photosynthetic pigments, and metabolite contents when compared to the control group. Seedlings from seeds treated with S04 displayed significant increases in shoot and root lengths, by 32.45% and 20.60% respectively, compared to the control group. Moreover, seedlings from seeds treated with S01 showed a 101.90% increase in total protein, whereas those treated with S02 experienced a 119.52% increase in carbohydrate content. These findings highlight the substantial improvements in growth characteristics, photosynthetic pigments, and metabolite levels in seedlings from treated seeds relative to controls. Total antioxidant capacity was boosted by CAP exposure. The activities of enzymes including superoxide dismutase, catalase, and peroxidases were stimulated by S02 and exceeded control treatment by (177.48%, 137.41%, and 103.32%), respectively. Additionally, exposure to S04 increased the levels of non-enzymatic antioxidants like flavonoids, phenolics, saponins, and tannins over the control group (38.08%, 30.10%, 117.19%, and 94.44%), respectively. Our results indicate that CAP-seed priming is an innovative and cost-effective approach to enhance the growth, bioactive components, and yield of tomato seedlings.


Assuntos
Antioxidantes , Gases em Plasma , Plântula , Solanum lycopersicum , Solanum lycopersicum/crescimento & desenvolvimento , Solanum lycopersicum/metabolismo , Solanum lycopersicum/efeitos dos fármacos , Plântula/crescimento & desenvolvimento , Plântula/efeitos dos fármacos , Plântula/metabolismo , Gases em Plasma/farmacologia , Antioxidantes/metabolismo , Fotossíntese/efeitos dos fármacos , Sementes/crescimento & desenvolvimento , Sementes/efeitos dos fármacos , Sementes/metabolismo
14.
Anticancer Res ; 44(6): 2437-2444, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38821624

RESUMO

BACKGROUND/AIM: Non-invasive physical plasma (NIPP) has shown promise in the treatment of cancer. However, conflicting results have been reported regarding the effect of NIPP on macrophage polarization. As tumor-associated macrophages (TAMs) are essential in the regulation of cancer development, this study aimed to determine the role of NIPP treatment in macrophage polarization and tumor-microenvironment (TME) remodeling. MATERIALS AND METHODS: A portable NIPP device, Plasma Care (Terraplasma Medical, Garching, Germany), was employed as the source of NIPP. The human monocytic cell line THP-1 was adopted as the cell model for macrophage differentiation and polarization. The effects of NIPP treatment on temperature, pH value, and oxidative stress induction of the culture medium were examined to validate the feasibility of applying the NIPP device in subsequent cell treatment. The changes in morphology, viability, and proliferation of THP-1 cells after NIPP treatment were determined. The expression of M1/M2 macrophage markers was examined by real-time quantitative polymerase chain reaction. RESULTS: No significant changes were observed in temperature and pH value after NIPP treatment, while the formation of hydrogen peroxide was promoted in a time-dependent manner. Cell morphology, viability, and proliferation were not affected by up to 6 minutes of NIPP treatment. In monocytes, 6 minutes of NIPP treatment significantly increased the expression of M1 markers (TNF-α and IL-6) and suppressed the M2 marker (CD206), findings which were consistent in the monocyte-derived macrophages. Furthermore, NIPP treatment also significantly promoted M1 polarization in the monocyte-derived macrophages induced by phorbol 12-myristate 13-acetate. CONCLUSION: NIPP is a safe and robust oxidative stress inducer and showed potential in TAM regulation by promoting M1 macrophage polarization.


Assuntos
Macrófagos , Gases em Plasma , Microambiente Tumoral , Humanos , Gases em Plasma/farmacologia , Macrófagos/metabolismo , Macrófagos/imunologia , Células THP-1 , Estresse Oxidativo , Diferenciação Celular , Proliferação de Células , Ativação de Macrófagos , Macrófagos Associados a Tumor/metabolismo , Macrófagos Associados a Tumor/imunologia
15.
World J Microbiol Biotechnol ; 40(7): 225, 2024 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-38822208

RESUMO

Ganoderma lucidum is known for its bioactive compounds, such as polysaccharides and triterpenoids, which are crucial in food and medicine. However, liquid fermentation encounters challenges in terms of strain differentiation and stability. In this research, we employed atmospheric room temperature plasma mutation and a microbial microdroplet culture system to identify strains with enhanced biomass and triterpenoid production. The three mutant strains, YB05, YB09, and YB18, exhibited accelerated growth rates and antagonized the initial strain G0023 more effectively than the controls. Notably, YB18 displayed the fastest growth, with a 17.25% increase in colony radius. Shake flask cultivation demonstrated that, compared with the initial strain, YB05 and YB18 had 26.33% and 17.85% greater biomass, respectively. Moreover, the triterpenoid production of YB05 and YB18 surpassed that of the control by 32.10% and 15.72%, respectively, as confirmed by colorimetric detection. Importantly, these mutant strains remained stable for five generations. This study revealed a comprehensive screening system utilizing atmospheric pressure, room temperature plasma mutation technology and microbial droplet cultivation. This innovative approach offers a promising pathway for obtaining advantageous Ganoderma strains for liquid fermentation. The methodology of atmospheric room temperature plasma mutation and microbial microdroplet culture systems is detailed for better comprehension.


Assuntos
Fermentação , Mutação , Reishi , Triterpenos , Reishi/crescimento & desenvolvimento , Reishi/metabolismo , Reishi/genética , Triterpenos/metabolismo , Biomassa , Temperatura , Gases em Plasma/farmacologia
16.
Sci Total Environ ; 939: 173447, 2024 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-38788942

RESUMO

Transmission of airborne infectious diseases poses great risk for public health and socio-economic stability, thus, there is a need for an effective control method targeting the spread and transmission of pathogenic aerosols. The existence of chemically-reductive trace air contaminants in animal agriculture may affect the oxidation inactivation process of pathogens. In this study, we report how the presence of such gasses impacts the effectiveness of using non-thermal plasma (NTP) within a packed-bed dielectric barrier discharge reactor to inactivate MS2 bacteriophage. Inactivation of the aerosolized bacteriophage is determined by the combination of viability and polymerase chain reaction assays. Using a plasma power source with a voltage of 20 kV and frequency of 350 Hz, after differentiating and excluding the physical removal effects of viral aerosols potentially caused by plasma, the baseline inactivation of MS2 aerosol in air has been determined based on an overall air flow rate of 200 Liters per minute and plasma discharge power of 1.8 W. When either ammonia or hydrogen sulfide gas is introduced into the airstream at a concentration of 1 part per million, the NTP virus inactivation efficiency is reduced to around 0.5-log from the 1-log baseline inactivation in air alone. Higher concentrations of those gasses will not further inhibit the effectiveness of plasma inactivation.


Assuntos
Microbiologia do Ar , Gases em Plasma , Inativação de Vírus , Inativação de Vírus/efeitos dos fármacos , Aerossóis , Levivirus/efeitos dos fármacos , Poluentes Atmosféricos
17.
Food Chem ; 453: 139711, 2024 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-38781893

RESUMO

The objective of the current work was to evaluate the impacts of dielectric barrier discharge plasma and repeated dry-heat treatments on the acetylation process of corn starch. The combined modification resulted in a higher substitution degree of acetate groups on starch chains compared to the acetylation treatment alone. This outcome was linked to the increase in surface area and structural organization level of granules achieved through the application of plasma and heating/cooling cycles, respectively. The successful esterification of starch structure was verified through FTIR (1710 cm-1) and 1H NMR (2 ppm). With the increase in plasma treatment duration up to 20 min, gelatinization enthalpy increased (10.81 J/g) due to the cross-linking reaction. Starch acetate produced through the combined treatment could find the application in the development of low-calorie food formulations due to its high resistant starch (70.5 g/100 g) and low viscosity (43 mPa s).


Assuntos
Temperatura Alta , Amido , Zea mays , Amido/química , Zea mays/química , Acetilação , Viscosidade , Gases em Plasma/química
18.
Int J Biol Macromol ; 270(Pt 1): 132346, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38750859

RESUMO

The impact of dual sequential modifications using radio-frequency (RF) plasma and electron beam irradiation (EBI) on starch properties was investigated and compared with single treatments within an irradiation dose range of 5-20 kGy. Regardless of sequence, dual treatments synergistically affected starch properties, increasing acidity, solubility, and paste clarity, while decreasing rheological features with increasing irradiation dose. The molecular weight distribution was also synergistically influenced. Amylopectin distribution broadened particularly below 10 kGy. Amylose narrowed its distribution across all irradiation doses. This was due to dominating EBI-induced degradation and molecular rearrangements from RF plasma. With the highest average radiation-chemical yield (G) and degradation rate constant (k) of (2.12 ± 0.14) × 10-6 mol·J-1 and (3.43 ± 0.23) × 10-4 kGy-1, respectively, upon RF plasma pre-treatment, amylose underwent random chain scission. In comparison to single treatments, dual modification caused minor alterations in spectral characteristics and crystal short-range order structure, along with increased granule aggregation and surface irregularities. The synergistic effect was dose-dependent, significant up to 10 kGy, irrespective of treatment sequence. The highest synergistic ratio was observed when RF plasma preceded irradiation, demonstrating the superior efficiency of plasma pre-treatment in combination with EBI. This synergy has the potential to lower costs and extend starch's technological uses by enhancing radiation sensitivity and reducing the irradiation dose.


Assuntos
Elétrons , Peso Molecular , Gases em Plasma , Amido , Amido/química , Gases em Plasma/farmacologia , Gases em Plasma/química , Solubilidade , Amilose/química , Amilopectina/química , Reologia
19.
Chemosphere ; 359: 142230, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38705412

RESUMO

This work studied the degradation reaction of sulfonated dyes, indigo carmine, phenol red, and their mixtures by non-thermal plasma (NTP). Interestingly, the degradation rate constant showed a faster process and lower activation energy (Ea) for the dye mixtures than for the degradation reaction of the individual dyes. This unexpected result opened up new opportunities for understanding plasma chemistry and the interaction between reactive species formed by the plasma and the target molecule. As no catalyst or chemical additive was added to the reactor, the decrease in Ea came from a self-synergistic effect (SSE), through the dye molecules fragmentation, which resulted in plasma catalysis. The hypothesis proposed in this work is that oxysulfur (SOx) species are formed by the desulfonation reaction of dyes. The sulfonic groups (SO3) present in the chemical structures of dyes can function as precursors for forming several SOx•- species. Studies based on oxygenated sulfonated species such as SO3•-, SO4•- and SO5•- have been widely applied in advanced oxidative and reductive processes due to their satisfactory efficiency and low cost. Among them, SO4•- is the key reactive species with the best performance in the degradation of pollutants due to its high oxidation potential (E° = 2.60 V). In addition, it is an alternative source of HO• in aqueous media, improving the oxidation reaction. In order to elucidate the SSE, the kinetic process was followed by UV-Vis analysis, and the reactive species, such as alkyl, hydroxyl, and oxy-sulfur radicals were identified by Electron Paramagnetic Resonance. The by-products of the NTP degradation reaction were analyzed by ultrafast liquid chromatography coupled with a mass spectrometer, and a fragmentation route was proposed.


Assuntos
Corantes , Gases em Plasma , Corantes/química , Catálise , Gases em Plasma/química , Oxirredução , Índigo Carmim/química , Poluentes Químicos da Água/química , Fenolsulfonaftaleína/química , Cinética , Ácidos Sulfônicos/química
20.
PLoS One ; 19(5): e0301216, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38743641

RESUMO

Non-thermal atmospheric-pressure plasma (NTAPP) has been widely studied for clinical applications, e.g., disinfection, wound healing, cancer therapy, hemostasis, and bone regeneration. It is being revealed that the physical and chemical actions of plasma have enabled these clinical applications. Based on our previous report regarding plasma-stimulated bone regeneration, this study focused on Achilles tendon repair by NTAPP. This is the first study to reveal that exposure to NTAPP can accelerate Achilles tendon repair using a well-established Achilles tendon injury rat model. Histological evaluation using the Stoll's and histological scores showed a significant improvement at 2 and 4 weeks, with type I collagen content being substantial at the early time point of 2 weeks post-surgery. Notably, the replacement of type III collagen with type I collagen occurred more frequently in the plasma-treated groups at the early stage of repair. Tensile strength test results showed that the maximum breaking strength in the plasma-treated group at two weeks was significantly higher than that in the untreated group. Overall, our results indicate that a single event of NTAPP treatment during the surgery can contribute to an early recovery of an injured tendon.


Assuntos
Tendão do Calcâneo , Gases em Plasma , Traumatismos dos Tendões , Cicatrização , Animais , Tendão do Calcâneo/lesões , Ratos , Gases em Plasma/farmacologia , Gases em Plasma/uso terapêutico , Cicatrização/efeitos dos fármacos , Traumatismos dos Tendões/terapia , Masculino , Hélio/farmacologia , Ratos Sprague-Dawley , Colágeno Tipo I/metabolismo , Resistência à Tração , Pressão Atmosférica , Colágeno Tipo III/metabolismo
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