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2.
Ultrason Sonochem ; 107: 106933, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38865900

RESUMO

Extraction of anthocyanins from Lycium ruthenicum Murr. (L. ruthenicum) is a notable challenge in food production, requiring methods that balance efficiency and safety. In this study, we conducted a comparative analysis the extraction of anthocyanins by natural air drying (NAD), vacuum freeze drying (VFD), hot air drying (HAD), and vacuum microwave drying (MVD) combined with ultrasonic-assisted enzymolysis extraction (UAEE). The results demonstrated that the extraction yield and antioxidant activity of anthocyanins were significantly higher in VFD. This phenomenon can be attributed to the modification of raw material's microstructure, leading to an increased extraction yield of specific anthocyanins such as Cyanidin-3-galactoside, Delphinidin chloride, Cyanidin, and Petunidin. According to the pretreatment results, the extraction process of anthocyanins was further optimized. The highest yield (3.16 g/100 g) was obtained in following conditions: 0.24 % pectinase, 48 °C, solid:liquid = 1:21, and 21 min ultrasonic time. This study improves the commercial value and potential application of L. ruthenicum in food industry.


Assuntos
Antocianinas , Dessecação , Lycium , Antocianinas/isolamento & purificação , Antocianinas/química , Lycium/química , Dessecação/métodos , Ondas Ultrassônicas , Fracionamento Químico/métodos , Antioxidantes/isolamento & purificação , Antioxidantes/química , Poligalacturonase , Micro-Ondas
3.
Food Funct ; 15(13): 6955-6965, 2024 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-38864520

RESUMO

In this study, we investigated the ameliorative gut modulatory effect of carboxymethylated Lycium barbarum seed dreg insoluble dietary fiber (LBSDIDF) on hyperlipidemic mice. After seven weeks of insoluble dietary fiber (IDF) intervention, the results demonstrated that IDFs effectively inhibited body weight gain, with slimming and hypolipidemic effects, and improved liver histopathology by decreasing ALT, AST, TNF-α and IL-6, and increasing short-chain fatty acid (SCFA) levels in hyperlipidemic mice. With the increasing diversity and abundance of intestinal bacteria and decreasing ratio of Firmicutes to Bacteroidetes, intestinal flora facilitated cholesterol lowering effects in hyperlipidemic mice. Our research offers a novel concept for the use of LBSDIDF as a prebiotic to improve intestinal dysbiosis or as a preventive measure against obesity and dyslipidemia.


Assuntos
Dieta Hiperlipídica , Fibras na Dieta , Microbioma Gastrointestinal , Hiperlipidemias , Lycium , Sementes , Animais , Camundongos , Dieta Hiperlipídica/efeitos adversos , Fibras na Dieta/farmacologia , Hiperlipidemias/tratamento farmacológico , Hiperlipidemias/dietoterapia , Sementes/química , Masculino , Lycium/química , Microbioma Gastrointestinal/efeitos dos fármacos , Camundongos Endogâmicos C57BL , Fígado/efeitos dos fármacos , Fígado/metabolismo , Intestinos/efeitos dos fármacos , Intestinos/microbiologia , Ácidos Graxos Voláteis/metabolismo , Humanos
4.
PLoS One ; 19(6): e0305111, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38870239

RESUMO

This study investigates the influence of cadmium (Cd) stress on the micropropagation of Goji Berry (Lycium barbarum L.) across three distinct genotypes (ERU, NQ1, NQ7), employing an array of machine learning (ML) algorithms, including Multilayer Perceptron (MLP), Support Vector Machines (SVM), Random Forest (RF), Gaussian Process (GP), and Extreme Gradient Boosting (XGBoost). The primary motivation is to elucidate genotype-specific responses to Cd stress, which poses significant challenges to agricultural productivity and food safety due to its toxicity. By analyzing the impacts of varying Cd concentrations on plant growth parameters such as proliferation, shoot and root lengths, and root numbers, we aim to develop predictive models that can optimize plant growth under adverse conditions. The ML models revealed complex relationships between Cd exposure and plant physiological changes, with MLP and RF models showing remarkable prediction accuracy (R2 values up to 0.98). Our findings contribute to understanding plant responses to heavy metal stress and offer practical applications in mitigating such stress in plants, demonstrating the potential of ML approaches in advancing plant tissue culture research and sustainable agricultural practices.


Assuntos
Cádmio , Lycium , Aprendizado de Máquina , Estresse Fisiológico , Cádmio/toxicidade , Lycium/efeitos dos fármacos , Lycium/crescimento & desenvolvimento , Estresse Fisiológico/efeitos dos fármacos , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/crescimento & desenvolvimento , Genótipo , Frutas/efeitos dos fármacos , Frutas/crescimento & desenvolvimento , Algoritmos
5.
BMC Genomics ; 25(1): 569, 2024 Jun 06.
Artigo em Inglês | MEDLINE | ID: mdl-38844874

RESUMO

BACKGROUND: Lycium is an economically and ecologically important genus of shrubs, consisting of approximately 70 species distributed worldwide, 15 of which are located in China. Despite the economic and ecological importance of Lycium, its phylogeny, interspecific relationships, and evolutionary history remain relatively unknown. In this study, we constructed a phylogeny and estimated divergence time based on the chloroplast genomes (CPGs) of 15 species, including subspecies, of the genus Lycium from China. RESULTS: We sequenced and annotated 15 CPGs in this study. Comparative analysis of these genomes from these Lycium species revealed a typical quadripartite structure, with a total sequence length ranging from 154,890 to 155,677 base pairs (bp). The CPGs was highly conserved and moderately differentiated. Through annotation, we identified a total of 128-132 genes. Analysis of the boundaries of inverted repeat (IR) regions showed consistent positioning: the junctions of the IRb/LSC region were located in rps19 in all Lycium species, IRb/SSC between the ycf1 and ndhF genes, and SSC/IRa within the ycf1 gene. Sequence variation in the SSC region exceeded that in the IR region. We did not detect major expansions or contractions in the IR region or rearrangements or insertions in the CPGs of the 15 Lycium species. Comparative analyses revealed five hotspot regions in the CPG: trnR(UCU), atpF-atpH, ycf3-trnS(GGA), trnS(GGA), and trnL-UAG, which could potentially serve as molecular markers. In addition, phylogenetic tree construction based on the CPG indicated that the 15 Lycium species formed a monophyletic group and were divided into two typical subbranches and three minor branches. Molecular dating suggested that Lycium diverged from its sister genus approximately 17.7 million years ago (Mya) and species diversification within the Lycium species of China primarily occurred during the recent Pliocene epoch. CONCLUSION: The divergence time estimation presented in this study will facilitate future research on Lycium, aid in species differentiation, and facilitate diverse investigations into this economically and ecologically important genus.


Assuntos
Evolução Molecular , Genoma de Cloroplastos , Lycium , Filogenia , Lycium/genética , Lycium/classificação , China , Variação Genética
6.
Food Res Int ; 188: 114502, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38823845

RESUMO

Lycium barbarum L. berries have a remarkable chemical composition and extensive biological activities, being a valuable component of health and nutraceutical practices. Nevertheless, a deep insight on the intestinal permeation of the pro-healthy bioactive compounds is urgently needed to predict the real effects on human body. This study attempted, for the first time, to optimize the Ultrasound-Assisted Extraction (UAE) of goji berries using a Response Surface Methodology approach and establish the intestinal permeation of the principal pro-healthy compounds. The optimal extraction conditions were a solid:liquid ratio of 8.75 % for 56.21 min, using an intensity of 59.05 W/m2. The optimal extract displayed a remarkable antioxidant capacity, with LC/DAD-ESI-MS analysis unveiled a diverse phytochemical profile, encompassing different compounds (e.g. glu-lycibarbarspermidine F, 2-glu-kukoamine, rutin, 3,5-dicaffeoylquinic acid). The intestinal co-culture model demonstrated that glu-lycibarbarspermidine F (isomer 2) (73.70 %), 3,5-dicaffeoylquinic acid (52.66 %), and isorhamnetin-3-O-rutinoside (49.31 %) traversed the intestinal cell layer, exerting beneficial health-promoting effects.


Assuntos
Antioxidantes , Frutas , Lycium , Extratos Vegetais , Lycium/química , Frutas/química , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Antioxidantes/farmacologia , Antioxidantes/isolamento & purificação , Humanos , Permeabilidade , Ondas Ultrassônicas , Compostos Fitoquímicos/isolamento & purificação , Mucosa Intestinal/metabolismo , Células CACO-2 , Absorção Intestinal , Rutina/isolamento & purificação , Ultrassom/métodos , Função da Barreira Intestinal
7.
PLoS One ; 19(5): e0302742, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38768144

RESUMO

Zeaxanthin dipalmitate (ZD) is a chemical extracted from wolfberry that protects degenerated photoreceptors in mouse retina. However, the pure ZD is expensive and hard to produce. In this study, we developed a method to enrich ZD from wolfberry on a production line and examined whether it may also protect the degenerated mouse retina. The ZD-enriched wolfberry extract (ZDE) was extracted from wolfberry by organic solvent method, and the concentration of ZD was identified by HPLC. The adult C57BL/6 mice were treated with ZDE or solvent by daily gavage for 2 weeks, at the end of the first week the animals were intraperitoneally injected with N-methyl-N-nitrosourea to induce photoreceptor degeneration. Then optomotor, electroretinogram, and immunostaining were used to test the visual behavior, retinal light responses, and structure. The final ZDE product contained ~30mg/g ZD, which was over 9 times higher than that from the dry fruit of wolfberry. Feeding degenerated mice with ZDE significantly improved the survival of photoreceptors, enhanced the retinal light responses and the visual acuity. Therefore, our ZDE product successfully alleviated retinal morphological and functional degeneration in mouse retina, which may provide a basis for further animal studies for possible applying ZDE as a supplement to treat degenerated photoreceptor in the clinic.


Assuntos
Modelos Animais de Doenças , Lycium , Camundongos Endogâmicos C57BL , Células Fotorreceptoras de Vertebrados , Extratos Vegetais , Degeneração Retiniana , Zeaxantinas , Animais , Lycium/química , Degeneração Retiniana/tratamento farmacológico , Degeneração Retiniana/patologia , Camundongos , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Zeaxantinas/farmacologia , Células Fotorreceptoras de Vertebrados/efeitos dos fármacos , Células Fotorreceptoras de Vertebrados/patologia , Eletrorretinografia , Retina/efeitos dos fármacos , Retina/patologia , Retina/metabolismo , Visão Ocular/efeitos dos fármacos , Masculino , Xantofilas/farmacologia
8.
J Agric Food Chem ; 72(22): 12752-12761, 2024 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-38779924

RESUMO

This study investigated the transformation of polyphenols, including free and bound polyphenols during the fermentation of wolfberry juice by Lactobacillus plantarum NCU137. Results indicated that fermentation significantly increased the free polyphenols content and released bound polyphenols, enhancing the antioxidant activity. Analysis showed that there were 19 free polyphenols, mainly scopoletin, pyrogallol, and dihydroferulic acid, and 16 bound polyphenols, especially p-coumaric acid, feruloyl hexoside, and caffeic acid. A significant correlation was observed between the generation and degradation of polyphenols, and specific bound polyphenols peaked during the 24-48 h fermentation. Furthermore, reduced surface roughness and galacturonic acid content in wolfberry residue, along with increased pectinase activity, suggested substantial pectin degradation in the cell wall, which may be associated with the release of polyphenols, due to pectin serving as carriers for bound polyphenols. The fermentation also increased polyphenol oxidase and peroxidase activity, contributing to polyphenol breakdown. These findings provide insights for improving wolfberry juice production.


Assuntos
Antioxidantes , Fermentação , Sucos de Frutas e Vegetais , Frutas , Lactobacillus plantarum , Lycium , Polifenóis , Lactobacillus plantarum/metabolismo , Lactobacillus plantarum/química , Polifenóis/metabolismo , Polifenóis/química , Antioxidantes/metabolismo , Antioxidantes/química , Sucos de Frutas e Vegetais/análise , Frutas/química , Frutas/metabolismo , Frutas/microbiologia , Lycium/química , Lycium/metabolismo , Pectinas/metabolismo , Pectinas/química
9.
Exp Eye Res ; 244: 109928, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38750781

RESUMO

The corneal epithelium, located as the outermost layer of the cornea, is inherently susceptible to injuries that may lead to corneal opacities and compromise visual acuity. Rapid restoration of corneal epithelial injury is crucial for maintaining the transparency and integrity of the cornea. Cell spray treatment emerges as an innovative and effective approach in the field of regenerative medicine. In our study, a cell spray printing platform was established, and the optimal printing parameters were determined to be a printing air pressure of 5 PSI (34.47 kPa) and a liquid flow rate of 30 ml/h. Under these conditions, the viability and phenotype of spray-printed corneal epithelial cells were preserved. Moreover, Lycium barbarum glycopeptide (LBGP), a glycoprotein purified from wolfberry, enhanced proliferation while simultaneously inhibiting apoptosis of the spray-printed corneal epithelial cells. We found that the combination of cell spray printing and LBGP facilitated the rapid construction of multilayered cell sheets on flat and curved collagen membranes in vitro. Furthermore, the combined cell spray printing and LBGP accelerated the recovery of the rat corneal epithelium in the mechanical injury model. Our findings offer a therapeutic avenue for addressing corneal epithelial injuries and regeneration.


Assuntos
Epitélio Corneano , Epitélio Corneano/efeitos dos fármacos , Epitélio Corneano/lesões , Animais , Ratos , Lesões da Córnea/tratamento farmacológico , Lesões da Córnea/patologia , Modelos Animais de Doenças , Cicatrização/efeitos dos fármacos , Cicatrização/fisiologia , Apoptose/efeitos dos fármacos , Ratos Sprague-Dawley , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Lycium/química , Bioimpressão/métodos , Impressão Tridimensional , Engenharia Tecidual/métodos , Glicoproteínas/farmacologia , Masculino , Medicamentos de Ervas Chinesas/farmacologia
10.
Sci Rep ; 14(1): 10586, 2024 05 08.
Artigo em Inglês | MEDLINE | ID: mdl-38719951

RESUMO

Carotenoids play essential roles in plant growth and development and provide plants with a tolerance to a series of abiotic stresses. In this study, the function and biological significance of lycopene ß-cyclase, lycopene ε-cyclase, and ß-carotene hydroxylase, which are responsible for the modification of the tetraterpene skeleton procedure, were isolated from Lycium chinense and analyzed. The overexpression of lycopene ß-cyclase, lycopene ε-cyclase, and ß-carotene hydroxylase promoted the accumulation of total carotenoids and photosynthesis enhancement, reactive oxygen species scavenging activity, and proline content of tobacco seedlings after exposure to the salt stress. Furthermore, the expression of the carotenoid biosynthesis genes and stress-related genes (ascorbate peroxidase, catalase, peroxidase, superoxide dismutase, and pyrroline-5-carboxylate reductase) were detected and showed increased gene expression level, which were strongly associated with the carotenoid content and reactive oxygen species scavenging activity. After exposure to salt stress, the endogenous abscisic acid content was significantly increased and much higher than those in control plants. This research contributes to the development of new breeding aimed at obtaining stronger salt tolerance plants with increased total carotenoids and vitamin A content.


Assuntos
Carotenoides , Regulação da Expressão Gênica de Plantas , Lycium , Nicotiana , Proteínas de Plantas , Tolerância ao Sal , Carotenoides/metabolismo , Nicotiana/genética , Nicotiana/metabolismo , Tolerância ao Sal/genética , Lycium/genética , Lycium/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/genética , Espécies Reativas de Oxigênio/metabolismo , Liases Intramoleculares/genética , Liases Intramoleculares/metabolismo , Fotossíntese/genética , Oxigenases de Função Mista/genética , Oxigenases de Função Mista/metabolismo , Ácido Abscísico/metabolismo
11.
J Hazard Mater ; 473: 134696, 2024 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-38788586

RESUMO

As medicine and food homology substance, goji berry is consumed worldwide in the form of fresh, dried and juice; however, pesticide residues have become one of the problems that essentially threaten its quality during cultivation and processing. In this study, a total of 75 dried goji berries were sampled from markets across China, and for the determination of 62 analytes, 28 pesticides were identified. Nine pesticides with high detectable rates and residual levels were selected for folia spraying, and their half-lives were found to range from 1.04 to 2.21 d. The processing factors (PFs) of juice were between 0.25 and 1.02, and this was mainly related with their octanol-water partition coefficient (logKow values). Washing could reduce pesticides residues to varying degrees with the removal rates between 17.00% and 74.05%. Sun drying with higher PF values in the range of 0.61-5.91 exhibited more obvious enrichment effect compared to oven drying. Commercial goji berry had cumulative chronic dietary risks with the hazard index (HI) values of 1.61%-4.97%. Its acute risk quotients (HQas) for consumers were 543.32%-585.92% and were mainly due to insecticides. These results provide important references for rationalizing pesticide application during goji berry cultivation and for the improvement of process to ensure food safety.


Assuntos
Contaminação de Alimentos , Frutas , Lycium , Resíduos de Praguicidas , Resíduos de Praguicidas/análise , Lycium/química , Contaminação de Alimentos/análise , Frutas/química , Manipulação de Alimentos , China , Medição de Risco
13.
BMC Plant Biol ; 24(1): 441, 2024 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-38778301

RESUMO

BACKGROUND: Goji (Lycium barbarum L.) is a perennial deciduous shrub widely distributed in arid and semiarid regions of Northwest China. It is highly valued for its medicinal and functional properties. Most goji varieties are naturally self-incompatible, posing challenges in breeding and cultivation. Self-incompatibility is a complex genetic trait, with ongoing debates regarding the number of self-incompatible loci. To date, no genetic mappings has been conducted for S loci or other loci related to self-incompatibility in goji. RESULTS: We used genome resequencing to create a high-resolution map for detecting de novo single-nucleotide polymorphisms (SNP) in goji. We focused on 229 F1 individuals from self-compatible '13-19' and self-incompatible 'new 9' varieties. Subsequently, we conducted a quantitative trait locus (QTL) analysis on traits associated with self-compatibility in goji berries. The genetic map consisted of 249,327 SNPs distributed across 12 linkage groups (LGs), spanning a total distance of 1243.74 cM, with an average interval of 0.002 cM. Phenotypic data related to self-incompatibility, such as average fruit weight, fruit rate, compatibility index, and comparable compatibility index after self-pollination and geitonogamy, were collected for the years 2021-2022, as well as for an extra year representing the mean data from 2021 to 2022 (2021/22). A total of 43 significant QTL, corresponding to multiple traits were identified, accounting for more than 11% of the observed phenotypic variation. Notably, a specific QTL on chromosome 2 consistently appeared across different years, irrespective of the relationship between self-pollination and geitonogamy. Within the localization interval, 1180 genes were annotated, including Lba02g01102 (annotated as an S-RNase gene), which showed pistil-specific expression. Cloning of S-RNase genes revealed that the parents had two different S-RNase alleles, namely S1S11 and S2S8. S-genotype identification of the F1 population indicated segregation of the four S-alleles from the parents in the offspring, with the type of S-RNase gene significantly associated with self-compatibility. CONCLUSIONS: In summary, our study provides valuable insights into the genetic mechanism underlying self-compatibility in goji berries. This highlights the importance of further positional cloning investigations and emphasizes the importance of integration of marker-assisted selection in goji breeding programs.


Assuntos
Mapeamento Cromossômico , Frutas , Lycium , Polimorfismo de Nucleotídeo Único , Locos de Características Quantitativas , Lycium/genética , Lycium/fisiologia , Frutas/genética , Frutas/fisiologia , Autoincompatibilidade em Angiospermas/genética , Fenótipo , China
14.
Molecules ; 29(10)2024 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-38792130

RESUMO

Lycium ruthenicum Murray possesses significant applications in both food and medicine, including antioxidative, anti-tumor, anti-fatigue, anti-inflammatory, and various other effects. Consequently, there has been a surge in research endeavors dedicated to exploring its potential benefits, necessitating the organization and synthesis of these findings. This article systematically reviews the extraction and content determination methods of active substances such as polysaccharides, anthocyanins, flavonoids, and polyphenols in LRM in the past five years, as well as some active ingredient composition determination methods, biological activities, and product development. This review is divided into three main parts: extraction and determination methods, their bioactivity, and product development. Building upon prior research, we also delve into the economic and medicinal value of Lycium ruthenicum Murray, thereby contributing significantly to its further exploration and development. It is anticipated that this comprehensive review will serve as a valuable resource for advancing research on Lycium ruthenicum Murray.


Assuntos
Lycium , Extratos Vegetais , Lycium/química , Extratos Vegetais/química , Antocianinas/química , Humanos , Flavonoides/química , Antioxidantes/química , Antioxidantes/farmacologia , Polifenóis/química , Compostos Fitoquímicos/química , Compostos Fitoquímicos/farmacologia , Polissacarídeos/química
15.
Sci Rep ; 14(1): 10856, 2024 05 13.
Artigo em Inglês | MEDLINE | ID: mdl-38740857

RESUMO

Bitter gourd, being perishable, requires timely harvesting. Delayed harvesting can result in a substantial reduction in fruit quality. while premature harvesting leads to underdeveloped fruit and decreased yields, the continuous flowering pattern in bitter gourd underscores the significance of accurately assessing fruit growth and ensuring timely harvesting for subsequent fruit setting and development. The current reliance on the experience of production personnel represents a substantial inefficiency. We present an improved real-time instance segmentation model based on YOLOv5-seg. The utilization of dynamic snake convolution enables the extraction of morphological features from the curved and elongated structure of bitter gourd. Diverse branch blocks enhance feature space diversity without inflating model size and inference time, contributing to improved recognition of expansion stages during bitter gourd growth. Additionally, the introduction of Focal-EIOU loss accurately locates the boundary box and mask, addressing sample imbalances in the L2 stage. Experimental results showcase remarkable accuracy rates of 99.3%, 93.8%, and 98.3% for L1, L2, and L3 stages using mAP@0.5. In comparison, our model outperforms other case segmentation models, excelling in both detection accuracy and inference speed. The improved YOLOv5-seg model demonstrates strong performance in fine-grained recognition of bitter gourd during the expansion stage. It efficiently segments bitter gourd in real-time under varying lighting and occlusion conditions, providing crucial maturity information. This model offers reliable insights for agricultural workers, facilitating precise harvesting decisions.


Assuntos
Frutas , Frutas/crescimento & desenvolvimento , Lycium/crescimento & desenvolvimento , Algoritmos
16.
Food Chem ; 453: 139640, 2024 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-38762945

RESUMO

Gas chromatography with mass spectrometry (GC/MS) and fractionation steps were used to determine the sterol patterns of red goji berries in detail. Twenty-five sterols were detected in fresh berries of two species (Lycium barbarum and L. chinense) from bushes grown in the botanical garden of the University of Hohenheim, and 20 sterols were identified. The rarely occurring campesta-5,24(25)-dienol, ß-sitosterol, Δ5-avenasterol, campesterol, and cycloartenol represented >60 % of the total sterol content. Maturity and drying of fresh red goji berries caused small changes but did not affect the characteristic sterol pattern. This was confirmed by analyzing various commercial dried red goji berry samples from different sources. Separated flesh and seed samples revealed pronounced differences in the sterol pattern. A new method of merging GC/MS chromatograms showed that ∼75 % of the sterols were present in seeds and ∼25 % in flesh. The unique sterol profile may be exploited to authenticate red goji berries.


Assuntos
Frutas , Cromatografia Gasosa-Espectrometria de Massas , Lycium , Esteróis , Frutas/química , Esteróis/análise , Lycium/química , Fitosteróis/análise , Extratos Vegetais/química
17.
Food Chem ; 453: 139659, 2024 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-38776792

RESUMO

There is a lack of research on how Tibetan kefir grains fermentation alters the physicochemical properties and biological activity of Lycium barbarum pulp polysaccharides, despite some reports that fermentation can affect the structure and activity of plant polysaccharides. This study demonstrated that, through fermentation, the molecular weight of polysaccharides decreased from 25.33 to 15.11 kg/mol while the contents of total sugar and uronic acid increased by 19.11% and 40.38%, respectively. Furthermore, after fermentation, the polysaccharides exhibited an uneven and rough surface along with a reduced number of branched chains and triple helix structures. Tibetan kefir grains fermentation enhanced the antioxidant activity of polysaccharides, which may be attributed to an increase in arabinose, galactose, and uronic acid content and a decrease in polysaccharide molecular weight. This research offers an alternative viewpoint on the potential application of Tibetan kefir grains-fermented Lycium barbarum pulp polysaccharides in functional foods.


Assuntos
Antioxidantes , Fermentação , Kefir , Lycium , Polissacarídeos , Lycium/química , Lycium/metabolismo , Antioxidantes/química , Antioxidantes/metabolismo , Antioxidantes/farmacologia , Kefir/microbiologia , Kefir/análise , Polissacarídeos/química , Polissacarídeos/metabolismo , Polissacarídeos/farmacologia , Peso Molecular , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Extratos Vegetais/metabolismo , Medicamentos de Ervas Chinesas
18.
Nat Commun ; 15(1): 4588, 2024 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-38816433

RESUMO

Lycibarbarspermidines are unusual phenolamide glycosides characterized by a dicaffeoylspermidine core with multiple glycosyl substitutions, and serve as a major class of bioactive ingredients in the wolfberry. So far, little is known about the enzymatic basis of the glycosylation of phenolamides including dicaffeoylspermidine. Here, we identify five lycibarbarspermidine glycosyltransferases, LbUGT1-5, which are the first phenolamide-type glycosyltransferases and catalyze regioselective glycosylation of dicaffeoylspermidines to form structurally diverse lycibarbarspermidines in wolfberry. Notably, LbUGT3 acts as a distinctive enzyme that catalyzes a tandem sugar transfer to the ortho-dihydroxy group on the caffeoyl moiety to form the unusual ortho-diglucosylated product, while LbUGT1 accurately discriminates caffeoyl and dihydrocaffeoyl groups to catalyze a site-selective sugar transfer. Crystal structure analysis of the complexes of LbUGT1 and LbUGT3 with UDP, combined with molecular dynamics simulations, revealed the structural basis of the difference in glycosylation selectivity between LbUGT1 and LbUGT3. Site-directed mutagenesis illuminates a conserved tyrosine residue (Y389 in LbUGT1 and Y390 in LbUGT3) in PSPG box that plays a crucial role in regulating the regioselectivity of LbUGT1 and LbUGT3. Our study thus sheds light on the enzymatic underpinnings of the chemical diversity of lycibarbarspermidines in wolfberry, and expands the repertoire of glycosyltransferases in nature.


Assuntos
Glicosiltransferases , Lycium , Glicosiltransferases/metabolismo , Glicosiltransferases/química , Glicosiltransferases/genética , Glicosilação , Lycium/enzimologia , Lycium/metabolismo , Lycium/química , Simulação de Dinâmica Molecular , Mutagênese Sítio-Dirigida , Proteínas de Plantas/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/química , Glicosídeos/metabolismo , Glicosídeos/química , Cristalografia por Raios X , Piperidinas/metabolismo , Piperidinas/química , Especificidade por Substrato
19.
Fish Shellfish Immunol ; 149: 109573, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38636742

RESUMO

This research elucidates the potential of Lycium barbarum residue (LBR), a by-product rich in bioactive substances, as a dietary supplement in aquaculture, especially for herbivorous fish like grass carp. In a detailed 120-day feeding trial, the impacts of varying LBR levels on juvenile grass carp were assessed, focusing on growth performance, survival rate, biochemical markers, and liver health. The study identified a 6% inclusion rate of LBR as optimal for enhancing survival and growth while mitigating hepatic lipid accumulation. Composition analysis of this diet revealed high concentrations of polysaccharides and flavonoids. Notably, the intake of LBR was found to enhance the antioxidant and immune-related enzymatic activities in the liver. Furthermore, it contributed to a reduction in hepatic fat deposition by decreasing the levels of triglycerides (TG) and total cholesterol (T-CHO) both in the liver and serum. Transcriptomic analysis of the liver highlighted LBR's substantial influence on lipid metabolism pathways, including the PPAR signaling pathway, primary bile acid biosynthesis, cholesterol metabolism, bile secretion, fat digestion and absorption, fatty acid degradation and fatty acid biosynthesis. Further, the expression level of genes pinpointed significant downregulation of fasn and dgat2, alongside upregulation of genes like pparda, cpt1b, cpt1ab and abca1b, in response to LBR supplementation. Overall, the findings present LBR as a promising enhancer of growth and survival in grass carp, with significant benefits in promoting fat metabolism and liver health, offering valuable insights for aquacultural nutrition strategies.


Assuntos
Ração Animal , Carpas , Dieta , Suplementos Nutricionais , Fígado , Animais , Carpas/crescimento & desenvolvimento , Carpas/imunologia , Ração Animal/análise , Fígado/metabolismo , Suplementos Nutricionais/análise , Dieta/veterinária , Metabolismo dos Lipídeos/efeitos dos fármacos , Lycium/química
20.
Acta Biochim Biophys Sin (Shanghai) ; 56(6): 844-856, 2024 06 25.
Artigo em Inglês | MEDLINE | ID: mdl-38606478

RESUMO

Lipid droplets (LDs) are dynamic organelles that store neutral lipids and are closely linked to obesity. Previous studies have suggested that Lycium barbarum polysaccharide (LBP) supplements can ameliorate obesity, but the underlying mechanisms remain unclear. In this study, we hypothesize that LBP alleviates LD accumulation in adipose tissue (AT) by inhibiting fat-specific protein 27 (Fsp27) through an activating transcription factor-6 (ATF6)/small-molecule sirtuin 1 (SIRT1)-dependent mechanism. LD accumulation in AT is induced in high-fat diet (HFD)-fed mice, and differentiation of 3T3-L1 preadipocytes (PAs) is induced. The ability of LBP to alleviate LD accumulation and the possible underlying mechanism are then investigated both in vivo and in vitro. The influences of LBP on the expressions of LD-associated genes ( ATF6 and Fsp27) are also detected. The results show that HFD and PA differentiation markedly increase LD accumulation in ATs and adipocytes, respectively, and these effects are markedly suppressed by LBP supplementation. Furthermore, LBP significantly activates SIRT1 and decreases ATF6 and Fsp27 expressions. Interestingly, the inhibitory effects of LBP are either abolished or exacerbated when ATF6 is overexpressed or silenced, respectively. Furthermore, SIRT1 level is transcriptionally regulated by LBP through opposite actions mediated by ATF6. Collectively, our findings suggest that LBP supplementation alleviates obesity by ameliorating LD accumulation, which might be partially mediated by an ATF6/SIRT1-dependent mechanism.


Assuntos
Células 3T3-L1 , Fator 6 Ativador da Transcrição , Tecido Adiposo , Medicamentos de Ervas Chinesas , Gotículas Lipídicas , Camundongos Endogâmicos C57BL , Sirtuína 1 , Animais , Sirtuína 1/metabolismo , Sirtuína 1/genética , Camundongos , Fator 6 Ativador da Transcrição/metabolismo , Fator 6 Ativador da Transcrição/genética , Tecido Adiposo/metabolismo , Tecido Adiposo/efeitos dos fármacos , Gotículas Lipídicas/metabolismo , Gotículas Lipídicas/efeitos dos fármacos , Masculino , Medicamentos de Ervas Chinesas/farmacologia , Dieta Hiperlipídica/efeitos adversos , Adipócitos/metabolismo , Adipócitos/efeitos dos fármacos , Obesidade/metabolismo , Obesidade/tratamento farmacológico , Lycium/química , Diferenciação Celular/efeitos dos fármacos , Metabolismo dos Lipídeos/efeitos dos fármacos
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