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1.
Int J Biol Macromol ; 266(Pt 1): 131198, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38552700

RESUMO

The metabolic process of polysaccharides in gastrointestinal digestions and the effects of the resulting carbohydrates on the composition of gut microbes are important to explore their prebiotic properties. Therefore, the purpose of this study was to investigate the simulated digestion and fecal fermentation in vitro of three fractions (PHEPSs-1, PHEPSs-2 and PHEPSs-3) purified from the crude exopolysaccharides of Paecilomyces hepiali HN1 (PHEPSs) and to explore the potential prebiotic mechanisms. The three purified fractions were characterized by HPLC, UV, FT-IR, SEM and AFM, and they were all of galactoglucomannan family with molecular weight of 178, 232 and 119 kDa, respectively. They could resist the simulated gastrointestinal digestions, but they were metabolized in fecal fermentation in vitro. Furthermore, the mannose in PHEPSs showed a higher utilization rate than that of glucose or galactose. The proliferation effects of PHEPSs on Bifidobacterium and Lactobacillus were weaker significantly than those of fructooligosaccharides before 12 h of fecal fermentation, but stronger after 24 h of fecal fermentation. Meanwhile, higher levels of short-chain fatty acids were found in PHEPSs groups when the fecal fermentation extended to 36 h. Therefore, PHEPSs are expected to have a potent gut healthy activity and can be explored as functional food ingredients.


Assuntos
Digestão , Fermentação , Microbioma Gastrointestinal , Paecilomyces , Humanos , Paecilomyces/metabolismo , Fezes/microbiologia , Polissacarídeos Fúngicos/química , Polissacarídeos Fúngicos/farmacologia , Prebióticos
3.
J Agric Food Chem ; 71(51): 20585-20601, 2023 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-38101321

RESUMO

Soil salinity is an important limiting factor in agricultural production. Rhizospheric fungi can potentially enhance crop salinity tolerance, but the precise role of signaling substances is still to be systematically elucidated. A rhizospheric fungus identified as Paecilomyces vaniformisi was found to enhance the salinity tolerance of rice seedlings. In this study, a novel polysaccharide (PPL2b) was isolated from P. vaniformisi and identified as consisting of Manp, Glcp, GalpA, and Galp. In a further study, PPL2b showed significant activity in alleviating salinity stress-induced growth inhibition in rice seedlings. The results indicated that under salinity stress, PPL2b enhances seed germination, plant growth (height and biomass), and biochemical parameters (soluble sugar and protein contents). Additionally, PPL2b regulates genes such as SOS1 and SKOR to decrease K+ efflux and increase Na+ efflux. PPL2b increased the expression and activity of genes related to antioxidant enzymes and nonenzyme substances in salinity-induced oxidative stress. Further study indicated that PPL2b plays a crucial role in regulating osmotic substances, such as proline and betaine, in maintaining the osmotic balance. It also modulates plant hormones to promote rice seedling growth and enhance their tolerance to soil salinity. The variables interacted and were divided into two groups (PC1 77.39% and PC2 18.77%) based on their relative values. Therefore, these findings indicate that PPL2b from P. vaniformisi can alleviate the inhibitory effects of salinity stress on root development, osmotic adjustment, ion balance, oxidative stress balance, and growth of rice seedlings. Furthermore, it suggests that polysaccharides produced by rhizospheric fungi could be utilized to enhance crop tolerance to salinity.


Assuntos
Oryza , Paecilomyces , Plântula , Oryza/metabolismo , Salinidade , Polissacarídeos/metabolismo , Solo/química , Estresse Fisiológico
4.
Mar Drugs ; 21(11)2023 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-37999419

RESUMO

A systematic investigation combined with a Global Natural Products Social (GNPS) molecular networking approach, was conducted on the metabolites of the deep-sea-derived fungus Samsoniella hepiali W7, leading to the isolation of three new fusaric acid derivatives, hepialiamides A-C (1-3) and one novel hybrid polyketide hepialide (4), together with 18 known miscellaneous compounds (5-22). The structures of the new compounds were elucidated through detailed spectroscopic analysis. as well as TD-DFT-based ECD calculation. All isolates were tested for anti-inflammatory activity in vitro. Under a concentration of 1 µM, compounds 8, 11, 13, 21, and 22 showed potent inhibitory activity against nitric oxide production in lipopolysaccharide (LPS)-activated BV-2 microglia cells, with inhibition rates of 34.2%, 30.7%, 32.9%, 38.6%, and 58.2%, respectively. Of particularly note is compound 22, which exhibited the most remarkable inhibitory activity, with an IC50 value of 426.2 nM.


Assuntos
Ácido Fusárico , Paecilomyces , Ácido Fusárico/farmacologia , Macrófagos , Anti-Inflamatórios , Estrutura Molecular
5.
Bioorg Chem ; 141: 106863, 2023 12.
Artigo em Inglês | MEDLINE | ID: mdl-37722269

RESUMO

Co-culturing the marine-derived fungi Penicillium janthinellium with Paecilomyces formosus led to the isolation of nine new indole-diterpenes, janthinellumines A-I (1-9), along with twelve known analogues (10-21). The chemical structures including their absolute configurations of them were assigned by the analysis of extensive spectroscopic data and calculated ECD and VCD methods. These indole-diterpenoids displayed extensive biological activities, including anti-influenza A virus, protein tyrosine phosphatase (PTP) inhibitory, and anti-Vibrio activities. Among them, the anti-influenza mechanism of compounds 1, 2, and 7 was further investigated using neuraminidase inhibitory assay, molecular docking, and reverse genetics methods, suggesting that 1, 2, and 7 could interact with Arg371 of the viral neuraminidase. The structure-activity relationship (SAR) of PTPs inhibitory activity for indole-diterpene derivatives (1, 2, 4, 5, 9-16, and 19-21) was also summarized.


Assuntos
Diterpenos , Paecilomyces , Penicillium , Simulação de Acoplamento Molecular , Técnicas de Cocultura , Neuraminidase/metabolismo , Indóis/química , Penicillium/química , Paecilomyces/metabolismo , Diterpenos/química , Estrutura Molecular
7.
J Dermatol ; 50(8): 1088-1092, 2023 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-37082787

RESUMO

Hyalohyphomycosis is a rare infection caused by a group of fungi that are devoid of pigments in their cell walls. As one of the main pathogens of hyalohyphomycosis, Purpureocillium lilacinum (former Paecilomyces lilacinus) is known for its intrinsic resistance to various antifungal agents. Here, we report three cases that coincide with a history of farming and all of them suffered from cutaneous hyalohyphomycosis caused by P. lilacinum. They shared a clinical presentation consisting of erythematous-to-violaceous painful plaques with overlying pustules on one of their forearms. Hyphae and fungal elements were highlighted by periodic acid Schiff or Gomori methenamine silver staining in their skin biopsies. Fungal cultures of their skin tissues yielded P. lilacinum, which was confirmed by both morphological and molecular characteristics. All patients responded well to oral terbinafine or itraconazole treatment. In this report, we also reviewed previously reported cases associated with either P. lilacinum or other Paecilomyces spp. infections in Taiwan.


Assuntos
Hialoifomicose , Paecilomyces , Humanos , Taiwan , Antifúngicos/uso terapêutico , Hialoifomicose/microbiologia , Celulite (Flegmão)/tratamento farmacológico
8.
Fitoterapia ; 166: 105443, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36736743

RESUMO

A new hybrid sorbicillinoid named paeciureallin (1) and a new monomeric sorbicillinoid named paecillyketide (2), along with six known analogues (3-8), were isolated from the rhizospheric soil-derived fungus Paecilomyces sp. KMU21009 associated with Delphinium yunnanense. Their structures were elucidated by extensive spectroscopic analysis and comparison with literature values. Paeciureallin (1) is the first example of hybrid sorbicillinoids possessing a rare sorbicillinoid urea unit and containing a ß-D-ribofuranose functionality. In pharmacological studies, compounds 1 and 2 were evaluated for in vitro anti-inflammatory and cytotoxic activities. Paeciureallin (1) exhibited moderate cytotoxicity against SW480 and A549 cell lines, and the IC50 values were 32.0 ± 0.1 and 34.4 ± 2.0 µM, respectively.


Assuntos
Antineoplásicos , Paecilomyces , Estrutura Molecular , Paecilomyces/química , Antineoplásicos/farmacologia , Anti-Inflamatórios
9.
Sci Rep ; 12(1): 19239, 2022 11 10.
Artigo em Inglês | MEDLINE | ID: mdl-36357560

RESUMO

The endophytic fungus Paecilomyces sp. (AUMC 15510) was isolated from healthy stem samples of the Egyptian medicinal plant Cornulaca monacantha. We used GC-MS and HPLC analysis to identify the bioactive constituents of ethyl acetate crude extract of Paecilomyces sp. (PsEAE). Six human microbial pathogens have been selected to evaluate the antimicrobial activity of PsEAE. Our data showed that the extract has significant antimicrobial activity against all tested pathogens. However, the best inhibitory effect was observed against Bacillus subtilis ATCC 6633 and Pseudomonas aeruginosa ATCC 90274 with a minimum inhibitory concentration (MIC) of 3.9 µg/ml and minimum bactericidal concentration (MBC) of 15.6 µg/ml, for both pathogens. Also, PsEAE exerts a significant inhibition on the biofilm formation of the previously mentioned pathogenic strains. In addition, we evaluated the wound healing efficiency of PsEAE on earthworms (Lumbricus castaneus) as a feasible and plausible model that mimics human skin. Interestingly, PsEAE exhibited a promising wound healing activity and enhanced wound closure. In conclusion, Paecilomyces sp. (AUMC 15510) could be a sustainable source of antimicrobial agents and a potential therapeutic target for wound management.


Assuntos
Anti-Infecciosos , Oligoquetos , Paecilomyces , Animais , Humanos , Extratos Vegetais/farmacologia , Anti-Infecciosos/farmacologia , Antibacterianos/farmacologia , Testes de Sensibilidade Microbiana , Cicatrização , Biofilmes
10.
Nutrients ; 14(14)2022 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-35889929

RESUMO

Paecilomyces hepiali, a fungal strain isolated from natural Ophiocordyceps sinensis, contains similar pharmacologically active components, has been used widely as a substitute of O. sinensis in functional food and medicine. However, the components and anti-fatigue effects of P.hepiali spores and their mechanisms of action are largely unknown. Here, we compared the chemical composition in P.hepiali spore (HPS) and mycelium (HPM) by liquid chromatography with tandem mass spectrometry analysis. We found 85 metabolites with significant differences, and HPS contains more L-Malic acid, Oxalacetic acid, Fructose-1,6-bisphosphate, and L-Arginine than HPM. Then we evaluated their anti-fatigue effects and regulatory effects on the gut microbiota in mice. The forced swimming time (SW) was only significantly increased in HPS groups: the high and low dose of the HPS group was 101% and 72% longer than the control group, respectively. Both HPS and HPM treatment decreased lactic acid, blood urea nitrogen, creatine kinase while increased lactate dehydrogenase (LDH) levels in the blood. Moreover, mice treated with HPS and HPM showed less skeletal muscle fiber spacing and breakage. The relative abundance of Alistips, Eubacterium, Bacterium, Parasutterella, and Olsenella in the gut microbiota of the HPS group was higher than that in the HPM group through 16S rRNA gene sequencing analysis. These changes may be related to the regulation of nucleotide, amino acid, and carbohydrate metabolism. Correlation analysis between the gut microbiota and fatigue-related indicators suggested that Alistips, Clostridium, Akkermansia, Olsenella, and Lactobacillus were positively correlated with the SW and LDH content. Our findings demonstrated that HPS has beneficial anti-fatigue effects by regulating gut microbiota.


Assuntos
Microbioma Gastrointestinal , Animais , Camundongos , Paecilomyces , Pós , RNA Ribossômico 16S , Esporos
11.
Appl Microbiol Biotechnol ; 106(12): 4787-4799, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35759038

RESUMO

Morels (Morchella spp.) are of great economic and scientific value. Paecilomyces penicillatus can cause white mold disease (WMD) widely emerging on morel ascocarps and is also a potential factor causing morel fructification failure. 1-octen-3-ol is a mushroom volatile compound with broad-spectrum antimicrobial activities. This study aimed to control the morel disease caused by P. penicillatus through suppressing P. penicillatus in the soil cultivated with Morchella sextelata using 1-octen-3-ol. Safe concentration of 1-octen-3-ol was estimated by comparing its inhibitory effect against P. penicillatus and M. sextelata, respectively, with mycelium-growth experiments on agar plates. The results showed that M. sextelata possesses a higher tolerance to 1-octen-3-ol than P. penicillatus with a 1-octen-3-ol concentration between 0 and 200 µL/L. Based on that, a sandy soil was supplemented with low (50 µL/L) or high concentration (200 µL/L) of 1-octen-3-ol. The effects of 1-octen-3-ol on soil microbial communities, WMD incidence, and morel yield were investigated. Compared to the non-supplemented control group, the incidence of WMD and the proportion of Paecilomyces in the soils of low- and high-concentration treatment groups were significantly decreased, corresponding to a significant increase in morel ascocarp yield. It suggests that 1-octen-3-ol effectively suppressed P. penicillatus in the soil, thereby reducing the severity of WMD and improving the morel yield. The diversity of soil bacterial communities was also altered by 1-octen-3-ol supplement. The proportion of Rhodococcus spp. in the soil was positively correlated with the 1-octen-3-ol concentration and ascocarp yield, suggesting its potential role in improving morel yield. KEY POINTS: • A novel method for morel disease suppression was proposed. • Paecilomyces in soil affects white mold disease and fructification yield of morel. • 1-Octen-3-ol suppresses Paecilomyces and alters bacterial community in soil.


Assuntos
Agaricales , Paecilomyces , Bactérias , Octanóis/farmacologia , Solo
13.
Nat Prod Res ; 36(19): 5053-5057, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33896278

RESUMO

Fungal pigments are important natural products with a wide range of applications. In this study, the purple-red pigment produced by the fungus Paecilomyces lilacinus TD16 (TD16 pigment) was separated with acidulated ethyl acetate and purified by silica gel column chromatography. Results of UV-visible spectrum and HPLC analyses showed that TD16 pigment is a new polyketide pigment with three absorption peaks at 228, 272 and 527 nm and a retention time of 11.4665 min distinct from those of other Paecilomyces-sourced pigments. Results of kinetic analysis and antimicrobial activity assay showed that TD16 pigment is a non-growth-associated secondary products with broad-spectrum antimicrobial activity on both bacteria and fungi and thus of potential application in industry.[Formula: see text].


Assuntos
Anti-Infecciosos , Produtos Biológicos , Paecilomyces , Policetídeos , Hypocreales , Cinética , Sílica Gel
16.
Appl Environ Microbiol ; 87(24): e0117721, 2021 11 24.
Artigo em Inglês | MEDLINE | ID: mdl-34586908

RESUMO

Fungi that degrade B20 biodiesel in storage tanks have also been linked to microbiologically influenced corrosion (MIC). A member of the filamentous fungal genus Paecilomyces and a yeast from the genus Wickerhamomyces were isolated from heavily contaminated B20 storage tanks from multiple Air Force bases. Although these taxa were linked to microbiologically influenced corrosion in situ, precise measurement of their corrosion rates and pitting severity on carbon steel was not available. In the experiments described here, we directly link fungal growth on B20 biodiesel to higher corrosion rates and pitting corrosion of carbon steel under controlled conditions. When these fungi were growing solely on B20 biodiesel for carbon and energy, consumption of FAME and n-alkanes was observed. The corrosion rates for both fungi were highest at the interface between the B20 biodiesel and the aqueous medium, where they acidified the medium and produced deeper pits than abiotic controls. Paecilomyces produced the most corrosion of carbon steel and produced the greatest pitting damage. This study characterizes and quantifies the corrosion of carbon steel by fungi that are common in fouled B20 biodiesel through their metabolism of the fuel, providing valuable insight for assessing MIC associated with storing and dispensing B20 biodiesel. IMPORTANCE Biodiesel is widely used across the United States and worldwide, blended with ultra-low-sulfur diesel in various concentrations. In this study, we were able to demonstrate that the filamentous fungus Paecilomyces AF001 and the yeast Wickerhamomyces SE3 were able to degrade fatty acid methyl esters and alkanes in biodiesel, causing increases in acidity. Both fungi also accelerated the corrosion of carbon steel, especially at the interface of the fuel and water, where their biofilms were located. This research provides controlled, quantified measurements and the localization of microbiologically influenced corrosion caused by common fungal contaminants in biodiesel fuels.


Assuntos
Biocombustíveis , Paecilomyces/metabolismo , Saccharomycetales/metabolismo , Aço , Alcanos , Biocombustíveis/microbiologia , Carbono , Corrosão
17.
Bioprocess Biosyst Eng ; 44(11): 2387-2398, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34268619

RESUMO

Cordycepin is a major bioactive compound found in Cordyceps sinensis that exhibits a broad spectrum of biological activities. Here a Paecilomyces hepiali OR-1 strain was initially isolated from plateau soil for the bioproduction of cordycepin. Subsequently, strain modification including 60Co γ-ray and ultraviolet irradiation were employed to increase the cordycepin titer, resulted in a high-yield mutant strain P. hepiali ZJB18001 with the cordycepin content of 0.61 mg/gDCW, showing a 2.3-fold to that from the wild strain (0.26 mg/gDCW). Furthermore, medium screening based on Box-Behnken design and the response surface methodology facilitated the enhancement of cordycepin yield to the value of 0.96 mg/gDCW at 25 °C for 5 days in submerged cultivation with an optimized medium composition. The high cordycepin yield, rapid growth rate and stable genetic characteristics of P. hepiali ZJB18001 are beneficial in terms of costs and time for the industrialization of cordycepin production.


Assuntos
Desoxiadenosinas/biossíntese , Mutação , Paecilomyces/metabolismo , Cordyceps/metabolismo , Meios de Cultura , Fermentação , Microscopia Eletrônica de Varredura , Paecilomyces/classificação , Paecilomyces/genética , Filogenia
18.
Acta Biomed ; 92(3): e2021139, 2021 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-34212928

RESUMO

Emergent pathogen as Purpureocillium lilacinum are becoming cause of morbidity and mortality in our population, especially in immunocompromised patients. We describe a case of hyalohyphomycosis in a diabetic man under systemic steroid treatment for a bullous pemphigoid. Treatment with different antimycotic drugs were ineffective and infection spread diffusely, leading to deterioration of general conditions and ultimately death. The aim of this article is to increase awareness of clinicians about this uncommon, but frequently fatal refractory mycotic infection.


Assuntos
Hialoifomicose , Paecilomyces , Penfigoide Bolhoso , Humanos , Hypocreales , Hospedeiro Imunocomprometido , Masculino , Penfigoide Bolhoso/tratamento farmacológico
19.
Environ Sci Pollut Res Int ; 28(47): 67429-67444, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34254237

RESUMO

Heavy metal accumulation in crop grains due to hazardous metal contamination is considered a great concern. However, phytobeneficial fungi are reported to have important abilities for the biosafety of crops grown in contaminated soil. Therefore, the current study was undertaken to explore the mutualistic association of plant growth-promoting endophytic fungi in reducing heavy metal concentration in the seeds of soybean plants subsequently grown in contaminated soil, without comprising seed quality and biochemical profile. The results revealed that endophytic Paecilomyces formosus LHL10 and Penicillium funiculosum LHL06 synergistically produced higher amounts of GAs and IAA in a co-cultured medium. Moreover, the co-inoculation of LHL06 and LHL10 to soybean plants grown under multi-metal toxic conditions significantly mitigated the adverse effects of heavy metal toxicity and increased the seed production (number of pods per plants, number of seeds per pod, and 100 seed weight) of soybean plants grown under control and multi-metal toxic conditions. Moreover, the levels of carbohydrates (glucose, sucrose, and fructose), minerals (iron, calcium, magnesium, and potassium), amino acids (serine, glutamic acids, glycine, methionine, lysine, arginine, and proline), and antioxidants (superoxide dismutase, catalase, and peroxidase) were significantly enhanced in sole and co-inoculated plants under control and stress conditions. Whereas organic acids (citric acid, tartaric acid, malic acid, and succinic acid), lipid peroxidation (MDA) products, multi-metal accumulation (nickel, cadmium, copper, lead, chromium, and aluminum), and stress-responsive endogenous abscisic acid levels were significantly decreased in seeds of soybean plants grown under control and multi-metal toxic conditions upon LHL06 and LHL10 sole and co-inoculation. The current results suggested the positive biochemical regulation in seeds for improving the nutritional status and making it safe for human consumption.


Assuntos
Paecilomyces , Poluentes do Solo , Endófitos , Humanos , Glycine max , Talaromyces
20.
J Virol Methods ; 297: 114246, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34329630

RESUMO

This study examined the anti-HSV-1 activity of EPS extracts isolated from mangrove fungus Paecilomyces Lilacinuson after intraperitoneal administration in mice. Mice were experimentally infected with HSV-1 intracranially and treated intraperitoneally with three different doses of EPS extract (6 g/Kg, 8 g/Kg, and 10 g/Kg) for 7 days. One group of 15 mice was infected with HSV-1 but did not receive any treatment, while another group of 15 mice was mock-infected to remain a control group. Animals were observed twice a day for 14 days after virus infection, searching for clinical signs of weight loss, piloerection, isolation, or retardation movement. Compared with the mock-infected group, mortality was significantly increased (p < 0.05) in the virus-infected group and the groups that received 6 g/Kg and 8 g/Kg EPS extract. Interestingly, no significant differences in mortality were found between the 10 g/Kg EPS extract and the mock-infected group. Mortality in the 10 g/Kg EPS extract group was substantially improved compared with virus-infected(p < 0.05). Additionally, EPS extracts inhibited HSV-1 replication in the mice brain in a dose-dependent manner. Furthermore, the extracts decreased NF-κB protein and mRNA expression and the production of TNF-α in HSV-1-infected mice brain tissue. These effects were also dose-dependent. Our findings suggest that the EPS extract may be a potential candidate for developing an antiviral drug against HSV-1.


Assuntos
Herpesvirus Humano 1 , Paecilomyces , Animais , Antivirais/farmacologia , Herpesvirus Humano 1/genética , Camundongos , NF-kappa B/metabolismo , NF-kappa B/farmacologia , Paecilomyces/metabolismo , Polissacarídeos , Fator de Necrose Tumoral alfa/metabolismo , Fator de Necrose Tumoral alfa/farmacologia
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