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1.
PLoS One ; 19(6): e0304679, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38848345

RESUMO

California yellowtail (CYT), Seriola dorsalis, is a promising candidate for aquaculture due to its rapid growth and high-quality flesh, particularly in markets like Japan, Australia, China, and the United States. Soy protein has shown success as a replacement for marine protein sources in CYT diets, reducing fishmeal levels, though concerns about potential intestinal inflammation persist with the inclusion of solvent-extracted soybean meal. To address this, processing strategies like fractionation, enzymatic treatment, heat treatment, and microbial fermentation have been employed to mitigate the negative impacts of soybean meal on fish nutrition and immune systems. This study focuses on optimizing soybean meal inclusion levels by incorporating advanced soy variants into CYT diets. The eight-week feeding trial, conducted in a recirculation system, featured six diets with sequential inclusion levels (0, 50, 100%) of high protein low oligosaccharide soybean meal (Bright Day, Benson Hill, St Louis, MO) and enzyme-treated soybean meal (HP 300, Hamlet Protein Inc., Findlay, OH), replacing solvent-extracted soybean. The study compares these formulations against a soy-free animal protein-based diet. At the end of the trial, fish were sampled for growth performance, body proximate composition, intestinal morphology, and immune response from gut samples. Results showed consistent FCR (P = 0.775), weight gain (P = 0.242), and high survival rate (99.4 ± 0.5%) among dietary treatments (P>0.05). Histological evaluations revealed no gut inflammation and gene expression analysis demonstrated no significant variations in immune, physiological, and digestive markers apn (P = 0.687), mga (P = 0.397), gpx1 (P = 0.279), atpase (P = 0.590), il1ß (P = 0.659). The study concludes that incorporating advanced soybean meal products, replacing up to 20% of fishmeal does not negatively affect CYT's growth and intestinal health. This suggests that all three soy sources, contributing 35% of total protein (15.4 g 100 g-1 diet), can be included in practical diets without compromising CYT's intestinal integrity or growth. These findings have positive implications for the commercial production of CYT and future research on the incorporation of plant-based proteins in aquaculture diets.


Assuntos
Ração Animal , Composição Corporal , Glycine max , Intestinos , Animais , Ração Animal/análise , Intestinos/efeitos dos fármacos , Intestinos/imunologia , Composição Corporal/efeitos dos fármacos , Dieta/veterinária , Perciformes/crescimento & desenvolvimento , Perciformes/imunologia , Perciformes/genética , Aquicultura/métodos , Fenômenos Fisiológicos da Nutrição Animal
2.
Food Res Int ; 188: 114492, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38823875

RESUMO

Two types of curcumin-loaded food-grade nano-silica (F-SiO2) hybrid materials were successfully synthesized using the rotary evaporation method (F-SiO2@Cur) and the adsorption method (Cur@F-SiO2). The microstructure and spectral analyses confirmed that the curcumin in F-SiO2@Cur was loaded within the nanopores in a non-aggregate form rather than being adsorbed onto the surface (Cur@F-SiO2). Additionally, F-SiO2@Cur exhibited remarkable water solubility (1510 ± 50.33 µg/mL) and photostability (a photodegradation ratio of only 59.22 %). Importantly, F-SiO2@Cur obtained a higher capacity for the generation of singlet oxygen (1O2) compared to control groups. Consequently, F-SiO2@Cur-mediated photodynamic inactivation (PDI) group attained the highest score in sensory evaluation and the best color protection effect in PDI experiment of small yellow croaker (Larimichthys polyactis) at 4 °C. Moreover, F-SiO2@Cur could effectively controlled total volatile basic nitrogen (TVB-N) content, pH, and total viable count (TVC), thereby prolonging the shelf life. Therefore, F-SiO2@Cur-mediated PDI is an effective fresh-keeping technology for aquatic products.


Assuntos
Curcumina , Conservação de Alimentos , Perciformes , Dióxido de Silício , Curcumina/farmacologia , Curcumina/química , Animais , Dióxido de Silício/química , Conservação de Alimentos/métodos , Nanopartículas , Alimentos Marinhos , Solubilidade , Oxigênio Singlete , Fotólise , Humanos
3.
Front Immunol ; 15: 1401626, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38868779

RESUMO

Zinc finger Asp-His-His-Cys motif-containing (zDHHC) proteins, known for their palmitoyltransferase (PAT) activity, play crucial roles in diverse cellular processes, including immune regulation. However, their non-palmitoyltransferase immunomodulatory functions and involvement in teleost immune responses remain underexplored. In this study, we systematically characterized the zDHHC family in the large yellow croaker (Larimichthys crocea), identifying 22 members. Phylogenetic analysis unveiled that each of the 22 LczDHHCs formed distinct clusters with their orthologues from other teleost species. Furthermore, all LczDHHCs exhibited a highly conserved DHHC domain, as confirmed by tertiary structure prediction. Notably, LczDHHC23 exhibited the most pronounced upregulation following Pseudomonas plecoglossicida (P. plecoglossicida) infection of macrophage/monocyte cells (MO/MΦ). Silencing LczDHHC23 led to heightened pro-inflammatory cytokine expression and diminished anti-inflammatory cytokine levels in MO/MΦ during infection, indicating its anti-inflammatory role. Functionally, LczDHHC23 facilitated M2-type macrophage polarization, as evidenced by a significant skewing of MO/MΦ towards the pro-inflammatory M1 phenotype upon LczDHHC23 knockdown, along with the inhibition of MO/MΦ necroptosis induced by P. plecoglossicida infection. These findings highlight the non-PAT immunomodulatory function of LczDHHC23 in teleost immune regulation, broadening our understanding of zDHHC proteins in host-pathogen interactions, suggesting LczDHHC23 as a potential therapeutic target for immune modulation in aquatic species.


Assuntos
Proteínas de Peixes , Macrófagos , Necroptose , Perciformes , Animais , Perciformes/imunologia , Macrófagos/imunologia , Macrófagos/metabolismo , Proteínas de Peixes/genética , Proteínas de Peixes/imunologia , Proteínas de Peixes/metabolismo , Necroptose/imunologia , Filogenia , Ativação de Macrófagos/imunologia , Doenças dos Peixes/imunologia , Doenças dos Peixes/microbiologia , Aciltransferases/genética , Aciltransferases/imunologia , Pseudomonas/fisiologia , Citocinas/metabolismo
4.
Sci Rep ; 14(1): 13320, 2024 06 10.
Artigo em Inglês | MEDLINE | ID: mdl-38858427

RESUMO

Climate change is intensifying extreme weather events, including marine heatwaves, which are prolonged periods of anomalously high sea surface temperature that pose a novel threat to aquatic animals. Tropical animals may be especially vulnerable to marine heatwaves because they are adapted to a narrow temperature range. If these animals cannot acclimate to marine heatwaves, the extreme heat could impair their behavior and fitness. Here, we investigated how marine heatwave conditions affected the performance and thermal tolerance of a tropical predatory fish, arceye hawkfish (Paracirrhites arcatus), across two seasons in Moorea, French Polynesia. We found that the fish's daily activities, including recovery from burst swimming and digestion, were more energetically costly in fish exposed to marine heatwave conditions across both seasons, while their aerobic capacity remained the same. Given their constrained energy budget, these rising costs associated with warming may impact how hawkfish prioritize activities. Additionally, hawkfish that were exposed to hotter temperatures exhibited cardiac plasticity by increasing their maximum heart rate but were still operating within a few degrees of their thermal limits. With more frequent and intense heatwaves, hawkfish, and other tropical fishes must rapidly acclimate, or they may suffer physiological consequences that alter their role in the ecosystem.


Assuntos
Recifes de Corais , Animais , Mudança Climática , Peixes/fisiologia , Perciformes/fisiologia , Aclimatação/fisiologia , Polinésia , Estações do Ano , Temperatura Alta , Frequência Cardíaca/fisiologia , Calor Extremo/efeitos adversos
5.
Sci Rep ; 14(1): 13820, 2024 06 15.
Artigo em Inglês | MEDLINE | ID: mdl-38879694

RESUMO

The Pama Croaker, Otolithoides pama, is an economically important fish species in Bangladesh. Intra-family similarities in morphology and typical barcode sequences of cox1 create ambiguities in its identification. Therefore, morphology and the complete mitochondrial genome of O. pama, and comparative mitogenomics within the family Sciaenidae have been studied. Extracted genomic DNA was subjected to Illumina-based short read sequencing for De-Novo mitogenome assembly. The complete mitogenome of O. pama (Accession: OQ784575.1) was 16,513 bp, with strong AC biasness and strand asymmetry. Relative synonymous codon usage (RSCU) among 13 protein-coding genes (PCGs) of O. pama was also analyzed. The studied mitogenomes including O. pama exhibited consistent sizes and gene orders, except for the genus Johnius which possessed notably longer mitogenomes with unique gene rearrangements. Different genetic distance metrics across 30 species of Sciaenidae family demonstrated 12S rRNA and the control region (CR) as the most conserved and variable regions, respectively, while most of the PCGs undergone a purifying selection. Different phylogenetic trees were congruent with one another, where O. pama was distinctly placed. This study would contribute to distinguishing closely related fish species of Sciaenidae family and can be instrumental in conserving the genetic diversity of O. pama.


Assuntos
Genoma Mitocondrial , Perciformes , Filogenia , Animais , Genoma Mitocondrial/genética , Perciformes/genética , Perciformes/classificação , Uso do Códon , Ordem dos Genes
6.
Ultrason Sonochem ; 107: 106945, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38857567

RESUMO

In this study, large yellow croaker (Larimichthys crocea) was frozen using multi-frequency ultrasound-assisted freezing (MUIF) with different powers (160 W, 175 W, and 190 W, respectively) and stored at -18 °C for ten months. The effect of different ultrasound powers on the myofibrillar protein (MP) structures and lipid oxidation of large yellow croaker was investigated. The results showed that MUIF significantly slowed down the oxidation of MP by inhibiting carbonyl formation and maintaining high sulfhydryl contents. These treatments also held a high activity of Ca2+-ATPase in the MP. MUIF maintained a higher ratio of α-helix to ß-sheet during frozen storage, thereby protecting the secondary structure of the tissue and stabilizing the tertiary structure. In addition, MUIF inhibited the production of thiobarbituric acid reactive substances value and the loss of unsaturated fatty acid content, indicating that MUIF could better inhibit lipid oxidation of large yellow croaker during long-time frozen storage.


Assuntos
Congelamento , Oxirredução , Perciformes , Animais , Fatores de Tempo , Armazenamento de Alimentos , Proteínas Musculares/química , Proteínas Musculares/metabolismo , Ondas Ultrassônicas , ATPases Transportadoras de Cálcio/metabolismo
7.
Dev Comp Immunol ; 157: 105191, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38705263

RESUMO

Ficus hirta Vahl. (FhV) has been shown to have antimicrobial and antiviral efficacy. To further ascertain the pharmacological properties of FhV., and to search for alternatives to antibiotics. An in vitro experiment was carried out to evaluate what influence FhV. would have on LPS-induced apoptosis. In this study, Fas, an apoptosis receptor, was cloned, which included a 5'-UTR of 39 bp, an ORF of 951 bp, a protein of 316 amino acids, and a 3'-UTR of 845 bp. EcFas was most strongly expressed in the spleen tissue of orange-spotted groupers. In addition, the apoptosis of fish spleen cells induced by LPS was concentration-dependent. Interestingly, appropriate concentrations of FhV. alleviated LPS-induced apoptosis. Inhibition of miR-411 further decreased the inhibitory effect of Fas on apoptosis, which reduced Bcl-2 expression and mitochondrial membrane potential, enhanced the protein expression of Bax and Fas. More importantly, the FhV. could activate miR-411 to improve this effect. In addition, luciferase reporter assays showed that miR-411 binds to Fas 3'-UTR to inhibit Fas expression. These findings provide evidence that FhV. alleviates LPS-induced apoptosis by activating miR-411 to inhibit Fas expression and, therefore, provided possible strategies for bacterial infections in fish.


Assuntos
Apoptose , Proteínas de Peixes , Lipopolissacarídeos , MicroRNAs , Baço , Animais , Apoptose/efeitos dos fármacos , Lipopolissacarídeos/imunologia , MicroRNAs/genética , MicroRNAs/metabolismo , Baço/metabolismo , Baço/imunologia , Proteínas de Peixes/metabolismo , Proteínas de Peixes/genética , Receptor fas/metabolismo , Receptor fas/genética , Doenças dos Peixes/imunologia , Regulação para Baixo , Bass/imunologia , Bass/genética , Células Cultivadas , Regiões 3' não Traduzidas/genética , Perciformes/imunologia
8.
Sci Total Environ ; 935: 173055, 2024 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-38723952

RESUMO

Anthropogenic noise is a global pollutant but its potential impacts on early life-stages in fishes are largely unknown. Here, using controlled laboratory experiments, we tested for impacts of continuous or intermittent exposure to low-frequency broadband noise on early life-stages of the common goby (Pomatoschistus microps), a marine fish with exclusive paternal care. Neither continuous nor intermittent noise exposure had an effect on filial cannibalism, showing that males were capable and willing to care for their broods. However, broods reared in continuous noise covered a smaller area and contained fewer eggs than control broods. Moreover, although developmental rate was the same in all treatments, larvae reared by males in continuous noise had, on average, a smaller yolk sac at hatching than those reared in the intermittent noise and control treatments, while larvae body length did not differ. Thus, it appears that the increased consumption of the yolk sac reserve was not utilised for increased growth. This suggests that exposure to noise in early life-stages affects fitness-related traits of surviving offspring, given the crucial importance of the yolk sac reserve during the early life of pelagic larvae. More broadly, our findings highlight the wide-ranging impacts of anthropogenic noise on aquatic wildlife living in an increasingly noisy world.


Assuntos
Ruído , Animais , Ruído/efeitos adversos , Masculino , Larva/crescimento & desenvolvimento , Comportamento Paterno , Perciformes
9.
Zoology (Jena) ; 164: 126172, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38749272

RESUMO

Vertebrates first emerged from water to land in the Paleozoic. Our understanding about the process has been steadily refined through paleontological studies, although the soft-body traits and behavior of these early animals remain poorly known. Mudskippers, extant amphibious gobies, could give insight into this question. This study reports on the ontogenetic transition from water to land of the mudskipper Periophthalmus modestus under laboratory conditions. After about 30 days after hatching (dah), the fish gradually changed their preference from water to an artificial shore and then to land. After about five days of periodic volitional emersion, the fish became able to propel themselves on land using the pectoral fins and after a further 13 days they began feeding on land. During the transition, the head morphology altered to suit for terrestrial existence. Tissue contents of triiodothyronine (T3) and thyroxine (T4) sharply increased at 30 dah. Forced underwater confinement of larvae at the last pelagic stage (27-29 dah) for 40-42 days resulted in no statistically significant difference in survival or gross morphology of the body and the gills. Growth was slightly stimulated. Our results show that mudskippers emerge on land with little morphological alteration during ontogenesis, much less than the changes observed for amphibians, and that emersion was not indispensable for survival or growth under our laboratory conditions. Further analysis of how and why mudskippers make their way across the water's edge will shed valuable light on what morphological, behavioral and physiological traits were needed for, and what environmental conditions may have driven the earliest steps of the water-to-land transition in ancient fishes.


Assuntos
Perciformes , Animais , Perciformes/fisiologia , Perciformes/crescimento & desenvolvimento , Perciformes/anatomia & histologia , Tri-Iodotironina , Tiroxina
10.
Bioinspir Biomim ; 19(4)2024 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-38722349

RESUMO

This study aims to investigate the feasibility of using an artificial lateral line (ALL) system for predicting the real-time position and pose of an undulating swimmer with Carangiform swimming patterns. We established a 3D computational fluid dynamics simulation to replicate the swimming dynamics of a freely swimming mackerel under various motion parameters, calculating the corresponding pressure fields. Using the simulated lateral line data, we trained an artificial neural network to predict the centroid coordinates and orientation of the swimmer. A comprehensive analysis was further conducted to explore the impact of sensor quantity, distribution, noise amplitude and sampling intervals of the ALL array on predicting performance. Additionally, to quantitatively assess the reliability of the localization network, we trained another neural network to evaluate error magnitudes for different input signals. These findings provide valuable insights for guiding future research on mutual sensing and schooling in underwater robotic fish.


Assuntos
Simulação por Computador , Sistema da Linha Lateral , Redes Neurais de Computação , Natação , Natação/fisiologia , Animais , Sistema da Linha Lateral/fisiologia , Modelos Biológicos , Perciformes/fisiologia , Robótica/instrumentação , Robótica/métodos , Hidrodinâmica , Biomimética/métodos
11.
Environ Res ; 252(Pt 4): 119129, 2024 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-38734292

RESUMO

Climate change has had a significant impact on many marine organisms. To investigate the effects of environmental changes on deep-water benthic fishes, we selected the genus Oplegnathus and applied species distribution modeling and ecological niche modeling. From the last glacial maximum to the present, the three Oplegnathus species (O. conwayi, O. robinsoni, and O. peaolopesi) distributed in the Cape of Good Hope region of southern Africa experienced fitness zone fluctuations of 39.9%, 13%, and 5.7%, respectively. In contrast, O. fasciatus and O. punctatus, which were primarily distributed in the western Pacific Ocean, had fitness zone fluctuations of -6.5% and 11.7%, respectively. Neither the O. insignis nor the O. woodward varied by more than 5% over the period. Under future environmental conditions, the range of variation in fitness zones for the three southern African Oplegnathus species was expected to be between -30.8% and -26.5%, while the range of variation in fitness zones for the two western Pacific stonefish species was expected to remain below 13%. In addition, the range of variation in the fitness zones of the O. insignis was projected to be between -2.3% and 7.1%, and the range of variation in the fitness zones of the O. woodward is projected to be between -5.7% and -2%. The results indicated that O. fasciatus and O. punctatus had a wide distribution and high expansion potential, while Oplegnathus species might have originated in western Pacific waters. Our results showed that benthic fishes were highly adaptable to extreme environments, such as the last glacial maximum. The high ecological niche overlap between Oplegnathus species in the same region suggested that they competed with each other. Future research could explore the impacts of environmental change on marine organisms and make conservation and management recommendations.


Assuntos
Mudança Climática , Ecossistema , Animais , Peixes/classificação , Peixes/fisiologia , Perciformes/fisiologia
12.
Genes (Basel) ; 15(5)2024 05 09.
Artigo em Inglês | MEDLINE | ID: mdl-38790234

RESUMO

It is widely known that all-female fish production holds economic value for aquaculture. Sebastes schlegelii, a preeminent economic species, exhibits a sex dimorphism, with females surpassing males in growth. In this regard, achieving all-female black rockfish production could significantly enhance breeding profitability. In this study, we utilized the widely used male sex-regulating hormone, 17α-methyltestosterone (MT) at three different concentrations (20, 40, and 60 ppm), to produce pseudomales of S. schlegelii for subsequent all-female offspring breeding. Long-term MT administration severely inhibits the growth of S. schlegelii, while short term had no significant impact. Histological analysis confirmed sex reversal at all MT concentrations; however, both medium and higher MT concentrations impaired testis development. MT also influenced sex steroid hormone levels in pseudomales, suppressing E2 while increasing T and 11-KT levels. In addition, a transcriptome analysis revealed that MT down-regulated ovarian-related genes (cyp19a1a and foxl2) while up-regulating male-related genes (amh) in pseudomales. Furthermore, MT modulated the TGF-ß signaling and steroid hormone biosynthesis pathways, indicating its crucial role in S. schlegelii sex differentiation. Therefore, the current study provides a method for achieving sexual reversal using MT in S. schlegelii and offers an initial insight into the underlying mechanism of sexual reversal in this species.


Assuntos
Metiltestosterona , Diferenciação Sexual , Animais , Metiltestosterona/farmacologia , Masculino , Feminino , Diferenciação Sexual/efeitos dos fármacos , Perciformes/genética , Perciformes/crescimento & desenvolvimento , Perciformes/metabolismo , Testículo/efeitos dos fármacos , Testículo/metabolismo , Testículo/crescimento & desenvolvimento , Peixes/genética , Peixes/crescimento & desenvolvimento , Peixes/metabolismo , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo
13.
Artigo em Inglês | MEDLINE | ID: mdl-38718893

RESUMO

The affect of temperature on tissue protein synthesis rates has been reported in temperate and tropical, but not Antarctic fishes. Previous studies have generally demonstrated low growth rates in Antarctic fish species in comparison to temperate relatives and elevated levels of protein turnover. This study investigates how low temperatures effect tissue protein synthesis and hence tissue growth in a polar fish species. Groups of Antarctic, Harpagifer antarcticus and temperate, Lipophrys pholis, were acclimated to a range of overlapping water temperatures and protein synthesis was measure in white muscle (WM), liver and gastrointestinal tract (GIT). WM protein synthesis rates increased linearly with temperature in both species (H. antarcticus 0.16-0.23%.d-1, L. pholis, 0.31-0.76%.d-1), while liver (H. antarcticus 0.24-0.27%.d-1, L. pholis, 0.44-1.03%.d-1) and GIT were unaffected by temperature in H. antarcticus but increased non-linearly in L.pholis (H. antarcticus 0.22-0.26%.d-1, L. pholis, 0.40-0.86%.d-1). RNA to protein ratios were unaffected by temperature in H. antarcticus but increased weakly, in L.pholis WM and liver. In L.pholis, RNA translational efficiency increased significantly with temperature in all tissues, but only in liver in H. antarcticus. At the overlapping temperature of 3 °C, protein synthesis (WM 26%, Liver, 39%, GIT, 35%) and RNA translational efficiency (WM 273%, Liver, 271%, GIT, 300%) were significantly lower in H. antarcticus than L.pholis, while RNA to protein ratios were significantly higher (WM 270%, Liver 170%, GIT 186%). Tissue specific effects of temperature are detectable in both species. This study provides the first evidence, that tissue protein synthesis rates are constrained in Antarctic fishes.


Assuntos
Fígado , Animais , Regiões Antárticas , Fígado/metabolismo , Biossíntese de Proteínas , Trato Gastrointestinal/metabolismo , Temperatura , Aclimatação , Proteínas de Peixes/metabolismo , Proteínas de Peixes/genética , Perciformes/metabolismo , Perciformes/genética
14.
Artigo em Inglês | MEDLINE | ID: mdl-38782254

RESUMO

Regional endothermy is the ability of an animal to elevate the temperature of specific regions of the body above that of the surrounding environment and has evolved independently among several fish lineages. Sarcolipin (SLN) is a small transmembrane protein that uncouples the sarcoplasmic reticulum calcium ATPase pump (SERCA1b) resulting in futile Ca2+ cycling and is thought to play a role in non-shivering thermogenesis (NST) in cold-challenged mammals and possibly some fishes. This study investigated the relative expression of sln and serca1 transcripts in three regionally-endothermic fishes (the skipjack, Katsuwonus pelamis, and yellowfin tuna, Thunnus albacares, both of which elevate the temperatures of their slow-twitch red skeletal muscle (RM) and extraocular muscles (EM), as well as the cranial endothermic swordfish, Xiphias gladius), and closely related ectothermic scombrids (the Eastern Pacific bonito, Sarda chiliensis, and Pacific chub mackerel, Scomber japonicus). Using Reverse Transcription quantitative PCR (RT-qPCR) and species-specific primers, relative sln expression trended higher in both the RM and EM for all four scombrid species compared to white muscle. In addition, relative serca1 expression was found to be higher in RM of skipjack and yellowfin tuna in comparison to white muscle. However, neither sln nor serca1 transcripts were higher in swordfish RM, EM or cranial heater tissue in comparison to white muscle. A key phosphorylation site in sarcolipin, threonine 5, is conserved in the swordfish, but is mutated to alanine or valine in tunas and the endothermic smalleye Pacific opah, Lampris incognitus, which should result in increased uncoupling of the SERCA pump. Our results support the role of potential SLN-NST in endothermic tunas and the lack thereof for swordfish.


Assuntos
Cálcio , Proteínas Musculares , Proteolipídeos , ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático , Termogênese , Animais , ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/genética , ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/metabolismo , Proteolipídeos/genética , Proteolipídeos/metabolismo , Proteínas Musculares/genética , Proteínas Musculares/metabolismo , Termogênese/genética , Cálcio/metabolismo , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Músculo Esquelético/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Perciformes/genética , Perciformes/fisiologia , Perciformes/metabolismo , Atum/genética , Atum/metabolismo , Atum/fisiologia
15.
Fish Shellfish Immunol ; 150: 109602, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38729252

RESUMO

Greater amberjack (Seriola dumerili) is a fish species that has significant economic and cultural value. It has a large size and grows rapidly. However, the intolerance to hypoxia poses a major obstacle to the growth of its aquaculture industry. This study focuses on the gills and spleen, two organs closely associated with the response to acute hypoxic stress. By simulating the acute hypoxic environment and using Illumina RNA-Seq technology, we explored the gills and spleen transcriptome changes in the acute hypoxia intolerant and tolerant groups of greater amberjack. It was discovered that gill tissues in the tolerant group may maintain a stable intracellular energy supply by promoting glycolysis and ß-oxidation compared to the intolerant group. Additionally, it promotes angiogenesis, enhances the ability to absorb dissolved oxygen, and accelerates oxygen transport to the mitochondria, adapting to the hypoxic environment. Anti-apoptotic genes were up-regulated in gill tissues in the tolerant group compared to the intolerant group, thereby minimizing the damage of acute hypoxia. On the other hand, the spleen inhibited the TCA and energy-consuming lipid synthesis pathways to supply energy under acute hypoxic stress. Pro-angiogenic genes were down-regulated in the spleen of individuals in the tolerant group compared to the intolerant group, which may be related to organ function. The suppressed reactive oxygen species (ROS) production and the impaired immune response function of the spleen were also found. The study explored the acute hypoxic stress response in greater amberjack and the molecular mechanisms underlying its tolerance to acute hypoxia.


Assuntos
Brânquias , Baço , Estresse Fisiológico , Animais , Baço/metabolismo , Baço/imunologia , Brânquias/metabolismo , Brânquias/imunologia , Hipóxia/genética , Hipóxia/veterinária , Regulação da Expressão Gênica/imunologia , Transcriptoma , Perciformes/genética , Perciformes/imunologia , Expressão Gênica , Peixes/genética , Peixes/imunologia
16.
Fish Shellfish Immunol ; 150: 109616, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38734118

RESUMO

Enteritis posed a significant health challenge to golden pompano (Trachinotus ovatus) populations. In this research, a comprehensive multi-omics strategy was implemented to elucidate the pathogenesis of enteritis by comparing both healthy and affected golden pompano. Histologically, enteritis was characterized by villi adhesion and increased clustering after inflammation. Analysis of the intestinal microbiota revealed a significant increase (P < 0.05) in the abundance of specific bacterial strains, including Photobacterium and Salinivibrio, in diseased fish compared to the healthy group. Metabolomic analysis identified 5479 altered metabolites, with significant impacts on terpenoid and polyketide metabolism, as well as lipid metabolism (P < 0.05). Additionally, the concentrations of several compounds such as calcitetrol, vitamin D2, arachidonic acid, and linoleic acid were significantly reduced in the intestines of diseased fish post-enteritis (P < 0.05), with the detection of harmful substances such as Efonidipine. In transcriptomic profiling, enteritis induced 68 upregulated and 73 downregulated genes, predominantly affecting steroid hormone receptor activity (P < 0.05). KEGG pathway enrichment analysis highlighted upregulation of SQLE and CYP51 in steroidogenesis, while the HSV-1 associated MHC1 gene exhibited significant downregulation. Integration of multi-omics results suggested a potential pathogenic mechanism: enteritis may have resulted from concurrent infection of harmful bacteria, specifically Photobacterium and Salinivibrio, along with HSV-1. Efonidipine production within the intestinal tract may have blocked certain calcium ion channels, leading to downregulation of MHC1 gene expression and reduced extracellular immune recognition. Upregulation of SQLE and CYP51 genes stimulated steroid hormone synthesis within cells, which, upon binding to G protein-coupled receptors, influenced calcium ion transport, inhibited immune activation reactions, and further reduced intracellular synthesis of anti-inflammatory substances like arachidonic acid. Ultimately, this cascade led to inflammation progression, weakened intestinal peristalsis, and villi adhesion. This study utilized multi-level omics detection to investigate the pathological symptoms of enteritis and proposed a plausible pathogenic mechanism, providing innovative insights into enteritis verification and treatment in offshore cage culture of golden pompano.


Assuntos
Enterite , Doenças dos Peixes , Microbioma Gastrointestinal , Animais , Enterite/veterinária , Enterite/imunologia , Enterite/microbiologia , Doenças dos Peixes/imunologia , Perfilação da Expressão Gênica/veterinária , Perciformes/imunologia , Perciformes/genética , Transcriptoma , Metabolômica , Multiômica
17.
Fish Shellfish Immunol ; 150: 109636, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38762095

RESUMO

As lower vertebrates, fish have both innate and adaptive immune systems, but the role of the adaptive immune system is limited, and the innate immune system plays an important role in the resistance to pathogen infection. C-type lectins (CLRs) are one of the major pattern recognition receptors (PRRs) of the innate immune system. CLRs can combine with pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs) to trigger NF-κB signaling pathway and exert immune efficacy. In this study, Ssclec12b and Ssclec4e of the C-type lectins, were found to be significantly up-regulated in the transcripts of Sebastes schlegelii macrophages stimulated by bacteria. The identification, expression and function of these lectins were studied. In addition, the recombinant proteins of the above two CLRs were obtained by prokaryotic expression. We found that rSsCLEC12B and rSsCLEC4E could bind to a variety of bacteria in a Ca2+-dependent manner, and promoted the agglutination of bacteria and blood cells. rSsCLEC12B and rSsCLEC4E assisted macrophages to recognize PAMPs and activate the NF-κB signaling pathway, thereby promoting the expression of inflammatory factors (TNF-α, IL-1ß, IL-6, IL-8) and regulating the early immune inflammation of macrophages. These results suggested that SsCLEC12B and SsCLEC4E could serve as PRRs in S. schlegelii macrophages to recognize pathogens and participate in the host antimicrobial immune process, and provided a valuable reference for the study of CLRs involved in fish innate immunity.


Assuntos
Doenças dos Peixes , Proteínas de Peixes , Imunidade Inata , Lectinas Tipo C , Macrófagos , Perciformes , Receptores de Reconhecimento de Padrão , Animais , Proteínas de Peixes/genética , Proteínas de Peixes/imunologia , Lectinas Tipo C/genética , Lectinas Tipo C/imunologia , Macrófagos/imunologia , Receptores de Reconhecimento de Padrão/genética , Receptores de Reconhecimento de Padrão/imunologia , Receptores de Reconhecimento de Padrão/metabolismo , Doenças dos Peixes/imunologia , Imunidade Inata/genética , Perciformes/imunologia , Perciformes/genética , Regulação da Expressão Gênica/imunologia , Perfilação da Expressão Gênica/veterinária , Peixes/imunologia , Peixes/genética
18.
Fish Shellfish Immunol ; 150: 109643, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38763177

RESUMO

The lymphocystis disease (LCD), caused by Lymphocystis disease virus (LCDV), is a benign and self-limiting disease described in a many freshwater and marine fish species. Hypertrophic fibroblasts and extensive aggregation of inflammatory cells are characteristics of LCD. In the present study, small animal imaging and ultrastructural investigations were carried out on the lymphocystis nodules of black rockfish (Sebastes schlegelii) naturally infected with lymphocystis iridovirus, to assess pathology, and the exudate with particular attention to the formation of extracellular traps (ETs) in vivo. Ex vivo were examined by nodules sections and primary cells stimulation. By histopathological analysis, the nodules contained infiltrated inflammatory cells and extensive basophilic fibrillar filaments at the periphery of the hypertrophied fibroblasts. ETs were assessed in nodules samples using indirect immunofluorescence to detect DNA and myeloperoxidase. Moreover, LCDV was able to infect peritoneal cells of black rockfish in vitro and induce the formation of ETs within 4 h. In summary, this study proved that ETs are involved in the response to LCDV infection and may be involved in formation of lymphoid nodules. Taken together, the findings provide a new perspective to determine the impact factors on the growth of nodules.


Assuntos
Infecções por Vírus de DNA , Armadilhas Extracelulares , Doenças dos Peixes , Iridoviridae , Perciformes , Animais , Doenças dos Peixes/virologia , Doenças dos Peixes/imunologia , Infecções por Vírus de DNA/veterinária , Infecções por Vírus de DNA/imunologia , Infecções por Vírus de DNA/virologia , Armadilhas Extracelulares/imunologia , Iridoviridae/fisiologia , Perciformes/imunologia , Pele/virologia , Pele/patologia , Peixes/imunologia , Peixes/virologia
19.
Fish Shellfish Immunol ; 150: 109644, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38777252

RESUMO

Enteritis poses a significant threat to fish farming, characterized by symptoms of intestinal and hepatic inflammation, physiological dysfunction, and dysbiosis. Focused on the leopard coral grouper (Plectropomus leopardus) with an enteritis outbreak on a South China Sea farm, our prior scrutiny did not find any abnormalities in feeding or conventional water quality factors, nor were any specific pathogen infections related to enteritis identified. This study further elucidates their intestinal flora alterations, host responses, and their interactions to uncover the underlying pathogenetic mechanisms and facilitate effective prevention and management strategies. Enteritis-affected fish exhibited substantial differences in intestinal flora compared to control fish (P = 0.001). Notably, norank_f_Alcaligenaceae, which has a negative impact on fish health, predominated in enteritis-affected fish (91.76 %), while the probiotic genus Lactococcus dominated in controls (93.90 %). Additionally, certain genera with pathogenesis potentials like Achromobacter, Sphingomonas, and Streptococcus were more abundant in diseased fish, whereas Enterococcus and Clostridium_sensu_stricto with probiotic potentials were enriched in control fish. At the transcriptomic level, strong inflammatory responses, accompanied by impaired metabolic functions, tissue damage, and iron death signaling activation were observed in the intestines and liver during enteritis. Furthermore, correlation analysis highlighted that potential pathogen groups were positively associated with inflammation and tissue damage genes while presenting negatively correlated with metabolic function-related genes. In conclusion, dysbiosis in the intestinal microbiome, particularly an aberrantly high abundance of Alcaligenaceae with pathogenic potential may be the main trigger for this enteritis outbreak. Alcaligenaceae alongside Achromobacter, Sphingomonas, and Streptococcus emerged as biomarkers for enteritis, whereas some species of Lactococcus, Clostridium_sensu_stricto, and Enterococcus showed promise as probiotics to alleviate enteritis symptoms. These findings enhance our understanding of enteritis pathogenesis, highlight intestinal microbiota shifts in leopard coral grouper, and propose biomarkers for monitoring, probiotic selection, and enteritis management.


Assuntos
Enterite , Doenças dos Peixes , Microbioma Gastrointestinal , Animais , Enterite/veterinária , Enterite/imunologia , Enterite/microbiologia , Doenças dos Peixes/imunologia , Doenças dos Peixes/microbiologia , Perciformes/imunologia , China , Expressão Gênica
20.
Food Res Int ; 187: 114323, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38763630

RESUMO

The balance regulation between characteristic aroma and hazards in high-temperature processed fish is a hot spot. This study was aimed to explore the interactive relationship between the nutritional value, microstructures, aroma, and harmful substances of hairtail under different frying methods including traditional frying (TF), air frying (AF), and vacuum frying (VF) via chemical pattern recognition. The results indicated that VF-prepared hairtail could form a crunchy mouthfeel and retain the highest content of protein (645.53 mg/g) and the lowest content of fat (242.03 mg/g). Vacuum frying reduced lipid oxidation in hairtail, resulting in the POV reaching 0.02 mg/g, significantly lower than that of TF (0.05 mg/g) and AF (0.21 mg/g), and TBARS reached 0.83 mg/g, significantly lower than that of AF (1.96 mg/g) (P < 0.05), respectively. Notable variations were observedin the aroma profileof hairtail preparedfrom different frying methods. Vacuum frying of hairtail resulted in higher levels of pyrazines and alcohols, whereas traditional frying and air frying were associated with the formation of aldehydes and ketones, respectively. Air frying was not a healthy way to cook hairtail which produced the highest concentration of harmful substances (up to 190.63 ng/g), significantly higher than VF (5.72 ng/g) and TF (52.78 ng/g) (P < 0.05), especially norharman (122.57 ng/g), significantly higher than VF (4.50 ng/g) and TF (32.63 ng/g) (P < 0.05). Norharman and acrylamide were the key harmful substances in hairtail treated with traditional frying. The vacuum frying method was an excellent alternative for deep-fried hairtail as a snack food with fewer harmful substances and a fine aroma, providing a theoretic guidance for preparing healthy hairtail food with high nutrition and superior sensory attraction.


Assuntos
Culinária , Temperatura Alta , Odorantes , Animais , Culinária/métodos , Odorantes/análise , Aldeídos/análise , Valor Nutritivo , Perciformes , Compostos Orgânicos Voláteis/análise , Pirazinas/análise , Pirazinas/química , Alimentos Marinhos/análise
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