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1.
Nihon Yakurigaku Zasshi ; 158(1): 35-38, 2023.
Artigo em Japonês | MEDLINE | ID: mdl-36596487

RESUMO

Depression is a common mental disorder and mainly characterized by persistent sadness and a lack of interest or pleasure in previously rewarding or enjoyable activities. Despair is also a common symptom of depression, and the forced swim and tail suspension tests are widely used to measure this behavior in rodents, but the results from these tests can include the effects on stress resistance in addition to depressive-like states. Reduced motivation is an important marker of psychiatric disorders, including depression, and thus we have previously developed the female encounter test, a novel and simple procedure for assessing reward-seeking behavior in adult male mice. Importantly, female mice should be considered in the development of animal models of depression and assessment of mouse behaviors since the lifetime prevalence of a major depressive disorder in women is almost twice that in men, and around one in seven women can develop postpartum depression. In this review, we summarized our recent research on the male encounter test for assessing motivation in adult female mice and introduced new topics on animal models and therapeutic drugs for postpartum depression.


Assuntos
Depressão Pós-Parto , Transtorno Depressivo Maior , Humanos , Camundongos , Feminino , Masculino , Animais , Natação/psicologia , Roedores , Depressão , Comportamento Animal , Modelos Animais de Doenças
2.
Emerg Infect Dis ; 29(2): 402-406, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36692446

RESUMO

Scrub typhus is a zoonosis caused by 3 species of Orientia bacteria, including Candidatus Orientia chuto. This species is known only from a human case in Dubai and infections in wildlife in Kenya. We report molecular detection of Candidatus O. chuto in 2 wild rodent species from Saudi Arabia.


Assuntos
Orientia tsutsugamushi , Tifo por Ácaros , Trombiculidae , Animais , Humanos , Animais Selvagens , Orientia tsutsugamushi/genética , Arábia Saudita/epidemiologia , Trombiculidae/microbiologia , Tifo por Ácaros/diagnóstico , Tifo por Ácaros/epidemiologia , Tifo por Ácaros/veterinária , Roedores
3.
Emerg Infect Dis ; 29(2): 433-434, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36692495

RESUMO

To investigate animal reservoirs of monkeypox virus in Nigeria, we sampled 240 rodents during 2018-2019. Molecular (real-time PCR) and serologic (IgM) evidence indicated orthopoxvirus infections, but presence of monkeypox virus was not confirmed. These results can be used to develop public health interventions to reduce human infection with orthopoxviruses.


Assuntos
Varíola dos Macacos , Orthopoxvirus , Infecções por Poxviridae , Animais , Humanos , Varíola dos Macacos/epidemiologia , Roedores , Nigéria/epidemiologia , Infecções por Poxviridae/epidemiologia , Infecções por Poxviridae/veterinária , Vírus da Varíola dos Macacos/genética , Orthopoxvirus/genética
4.
Curr Biol ; 33(2): R63-R65, 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36693309

RESUMO

A new study shows that Vanilla species are unique among orchids for having evolved a multimodal mechanism of seed and fruit dispersal. Bees inadvertently transport seeds externally while collecting fragrance or nest material from dehiscent fruits. Rodents and marsupials consume and disperse aromatic fleshy fruits and the crustose seeds within.


Assuntos
Dispersão de Sementes , Vanilla , Animais , Frutas , Sementes , Mamíferos , Insetos , Roedores , Comportamento Alimentar
5.
Parasit Vectors ; 16(1): 27, 2023 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-36694253

RESUMO

BACKGROUND: Hepatozoon spp. are tick-borne parasites causing subclinical to clinical disease in wild and domestic animals. Aim of this study was to determine Hepatozoon prevalence and species distribution among wild mammals and ticks in Europe. METHODS: Samples of wild mammals and ticks, originating from Austria, Bosnia and Herzegovina, Croatia, Belgium and the Netherlands, were tested with PCR to amplify a ~ 670-bp fragment of the small subunit ribosomal RNA gene. RESULTS: Of the 2801 mammal samples that were used for this study, 370 (13.2%) tested positive. Hepatozoon canis was detected in samples of 178 animals (3 Artiodactyla, 173 Carnivora, 1 Eulipotyphia, 1 Lagomorpha), H. martis in 125 (3 Artiodactyla, 122 Carnivora), H. sciuri in 13 (all Rodentia), Hepatozoon sp. in 47 (among which Hepatozoon sp. Vole isolate, all Rodentia) and H. ayorgbor in 4 (all Rodentia). Regarding origin, 2.9% (6/208) tested positive from Austria, 2.8% (1/36) from Bosnia and Herzegovina, 14.6% (173/1186) from Croatia and 13.9% (190/1371) from Belgium/the Netherlands. Of the 754 ticks collected, 0.0% (0/35) Hyalomma sp., 16.0% (4/25) Dermacentor spp., 0.0% (0/23) Haemaphysalis spp., 5.3% (24/50) Ixodes and 1.4% (3/221) Rhipicephalus spp. tested positive for Hepatozoon (4.2%; 32/754), most often H. canis (n = 22). CONCLUSIONS: Hepatozoon canis is most present in mammals (especially in Carnivora such as gray wolves and golden jackals) and ticks, followed by H. martis, which was found merely in stone martens and pine martens. None of the rodent-associated Hepatozoon spp. were detected in the ticks, suggesting the possible implication of other arthropod species or non-vectorial routes in the transmission cycle of the hemoprotozoans in rodents. Our findings of H. canis in ticks other than R. sanguineus add to the observation that other ticks are also involved in the life cycle of Hepatozoon. Now that presence of Hepatozoon has been demonstrated in red foxes, gray wolves, mustelids and rodents from the Netherlands and/or Belgium, veterinary clinicians should be aware of the possibility of spill-over to domestic animals, such as dogs.


Assuntos
Coccidiose , Eucoccidiida , Ixodes , Ixodidae , Mustelidae , Lobos , Cães , Animais , Coccidiose/epidemiologia , Coccidiose/veterinária , Coccidiose/parasitologia , Eucoccidiida/genética , Ixodes/parasitologia , Ixodidae/parasitologia , Raposas/parasitologia , Europa (Continente)/epidemiologia , Roedores , Animais Domésticos
6.
PLoS Negl Trop Dis ; 17(1): e0011051, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36634106

RESUMO

BACKGROUND: Scrub typhus is a potentially severe infection caused by bacteria of the genus Orientia, endemic in Asia-Pacific and recently discovered in southern Chile. The presented study aimed to determine the prevalence and species richness of rodent-associated trombiculid mites and their infection with Orientia spp. in different areas of two regions in southern Chile. METHODOLOGY/PRINCIPAL FINDINGS: During summer 2020, trombiculid mites were collected from rodents captured in three areas in southern Chile known to be endemic for scrub typhus (Cochamó and Chiloé Island in the Los Lagos Region and Tortel in the Aysén Region). A total of 132 rodents belonging to five species were captured using Sherman-like traps; 89.4% were infested with trombiculids. Mite specimens were morphologically identified and subsequently tested by Orientia-specific qPCR. Six mite species were identified. Among chigger-infested rodents, 33.9% carried Orientia-positive mites; this rate was higher in Tortel (63.8%) than in Cochamó (45.0%) and Chiloé Island (2.0%). The analysis of individual mites (n = 901) revealed that 31.2% of Herpetacarus antarctica samples (n = 202) were positive for Orientia DNA; the prevalence was 7.0% in Paratrombicula neuquenensis (n = 213), 6.9% in Herpetacarus eloisae (n = 144), 3.6% in Argentinacarus expansus (n = 55), and 0% in Paratrombicula goffi (n = 110) and Quadraseta chiloensis (n = 177). The southernmost site (Tortel) showed the highest rates of trombiculid infestation, trombiculid load, and Orientia infection in the captured rodents. CONCLUSIONS/SIGNIFICANCE: Our study provides new insights into the trombiculid fauna and prevalence of Orientia in mites collected from wild rodents in southern Chile. Orientia DNA was detected in four of the six mite species. Rates of infestation, mite loads, and Orientia prevalences differed geographically and were highest in the Aysén Region. Our data improve our knowledge on possible vectors of scrub typhus and their distribution in Chile.


Assuntos
Orientia tsutsugamushi , Tifo por Ácaros , Trombiculidae , Animais , Tifo por Ácaros/epidemiologia , Tifo por Ácaros/microbiologia , Roedores , Trombiculidae/microbiologia , Orientia tsutsugamushi/genética , Orientia , Chile/epidemiologia
7.
Nat Commun ; 14(1): 282, 2023 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-36650127

RESUMO

Striatal projection neurons (SPNs), which progressively degenerate in human patients with Huntington's disease (HD), are classified along two axes: the canonical direct-indirect pathway division and the striosome-matrix compartmentation. It is well established that the indirect-pathway SPNs are susceptible to neurodegeneration and transcriptomic disturbances, but less is known about how the striosome-matrix axis is compromised in HD in relation to the canonical axis. Here we show, using single-nucleus RNA-sequencing data from male Grade 1 HD patient post-mortem brain samples and male zQ175 and R6/2 mouse models, that the two axes are multiplexed and differentially compromised in HD. In human HD, striosomal indirect-pathway SPNs are the most depleted SPN population. In mouse HD models, the transcriptomic distinctiveness of striosome-matrix SPNs is diminished more than that of direct-indirect pathway SPNs. Furthermore, the loss of striosome-matrix distinction is more prominent within indirect-pathway SPNs. These results open the possibility that the canonical direct-indirect pathway and striosome-matrix compartments are differentially compromised in late and early stages of disease progression, respectively, differentially contributing to the symptoms, thus calling for distinct therapeutic strategies.


Assuntos
Doença de Huntington , Camundongos , Animais , Humanos , Masculino , Doença de Huntington/genética , Doença de Huntington/metabolismo , Roedores , Corpo Estriado/metabolismo , Neurônios/metabolismo , Gânglios da Base/metabolismo , Modelos Animais de Doenças , Camundongos Transgênicos
8.
Int J Mol Sci ; 24(2)2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36674885

RESUMO

Our previous study reported that mesenchymal stem cells (MSCs) accelerated the wound healing process through anti-inflammatory, anti-apoptotic, and pro-angiogenetic effects in a rodent skin excision model. NF3 is a twin-herb formula, which presents similar effects in promoting wound healing. Research focusing on the interaction of MSCs and Chinese medicine is limited. In this study, we applied MSCs and the twin-herb formula to the wound healing model and investigated their interactions. Wound healing was improved in all treatment groups (MSCs only, NF3 only, and MSCs + NF3). The combined therapy further enhanced the effect: more GFP-labelled ADMSCs, collagen I and collagen III expression, Sox9 positive cells, and CD31 positive cells, along with less ED-1 positive cells, were detected; the expressions of proinflammatory cytokine IL-6 and TNF-α were downregulated; and the expression of anti-inflammatory cytokine IL-10 was upregulated. In vitro, NF3 promoted the cell viability and proliferation ability of MSCs, and a higher concentration of protein was detected in the NF3-treated supernatant. A proteomic analysis showed there were 15 and 22 proteins in the supernatants of normal ADMSCs and NF3-treated ADMSCs, respectively. After PCR validation, the expressions of 11 related genes were upregulated. The results of a western blot suggested that the TGFß/Smad and Wnt pathways were related to the therapeutic effects of the combined treatment. Our study suggests for the first time that NF3 enhanced the therapeutic effect of MSCs in the wound healing model and the TGFß/Smad and Wnt pathways were related to the procedure.


Assuntos
Medicamentos de Ervas Chinesas , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais , Animais , Medicamentos de Ervas Chinesas/farmacologia , Roedores , Proteômica , Cicatrização , Colágeno/farmacologia , Citocinas/farmacologia , Fator de Crescimento Transformador beta/farmacologia , Anti-Inflamatórios/farmacologia
9.
Curr Top Microbiol Immunol ; 439: 265-303, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36592249

RESUMO

Members of the family Arenaviridae are classified into four genera: Antennavirus, Hartmanivirus, Mammarenavirus, and Reptarenavirus. Reptarenaviruses and hartmaniviruses infect (captive) snakes and have been shown to cause boid inclusion body disease (BIBD). Antennaviruses have genomes consisting of 3, rather than 2, segments, and were discovered in actinopterygian fish by next-generation sequencing but no biological isolate has been reported yet. The hosts of mammarenaviruses are mainly rodents and infections are generally asymptomatic. Current knowledge about the biology of reptarenaviruses, hartmaniviruses, and antennaviruses is very limited and their zoonotic potential is unknown. In contrast, some mammarenaviruses are associated with zoonotic events that pose a threat to human health. This review will focus on mammarenavirus genetic diversity and its biological implications. Some mammarenaviruses including lymphocytic choriomeningitis virus (LCMV) are excellent experimental model systems for the investigation of acute and persistent viral infections, whereas others including Lassa (LASV) and Junin (JUNV) viruses, the causative agents of Lassa fever (LF) and Argentine hemorrhagic fever (AHF), respectively, are important human pathogens. Mammarenaviruses were thought to have high degree of intra-and inter-species amino acid sequence identities, but recent evidence has revealed a high degree of mammarenavirus genetic diversity in the field. Moreover, closely related mammarenavirus can display dramatic phenotypic differences in vivo. These findings support a role of genetic variability in mammarenavirus adaptability and pathogenesis. Here, we will review the molecular biology of mammarenaviruses, phylogeny, and evolution, as well as the quasispecies dynamics of mammarenavirus populations and their biological implications.


Assuntos
Arenaviridae , Animais , Humanos , Arenaviridae/genética , Arenaviridae/metabolismo , Roedores , Variação Genética
10.
Int J Mol Sci ; 24(2)2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36675269

RESUMO

Cowpox is caused by a DNA virus known as the cowpox virus (CPXV) belonging to the Orthopoxvirus genus in the family Poxviridae. Cowpox is a zoonotic disease with the broadest host range among the known poxviruses. The natural reservoir hosts of CPXV are wild rodents. Recently, the cases of orthopoxviral infections have been increasing worldwide, and cowpox is considered the most common orthopoxviral infection in Europe. Cowpox is often a self-limiting disease, although cidofovir or anti-vaccinia gammaglobulin can be used in severe and disseminated cases of human cowpox. In this computational study, a molecular docking analysis of thymine- and arabinofuranosyl-thymine-related structures (1-21) on two cowpox-encoded proteins was performed with respect to the cidofovir standard and a 3D ligand-based pharmacophore model was generated. Three chemical structures (PubChem IDs: 123370001, 154137224, and 90413364) were identified as potential candidates for anti-cowpox agents. Further studies combining in vitro and in silico molecular dynamics simulations to test the stability of these promising compounds could effectively improve the future design of cowpox virus inhibitors, as molecular docking studies are not sufficient to consider a ligand a potential drug.


Assuntos
Vírus da Varíola Bovina , Varíola Bovina , Animais , Humanos , Vírus da Varíola Bovina/genética , Vírus da Varíola Bovina/metabolismo , Timina/metabolismo , Cidofovir/farmacologia , Ligantes , Simulação de Acoplamento Molecular , Roedores
13.
J Neurosci Methods ; 386: 109779, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36621552

RESUMO

BACKGROUND: Touchscreen-based behavioral assays provide a robust method for assessing cognitive behavior in rodents, offering great flexibility and translational potential. The development of touchscreen assays presents a significant programming and mechanical engineering challenge, where commercial solutions can be prohibitively expensive and open-source solutions are underdeveloped, with limited adaptability. NEW METHOD: Here, we present Visiomode (www.visiomode.org), an open-source platform for building rodent touchscreen-based behavioral tasks. Visiomode leverages the inherent flexibility of touchscreens to offer a simple yet adaptable software and hardware platform. The platform is built on the Raspberry Pi computer combining a web-based interface and powerful plug-in system with an operant chamber that can be adapted to generate a wide range of behavioral tasks. RESULTS: As a proof of concept, we use Visiomode to build both simple stimulus-response and more complex visual discrimination tasks, showing that mice display rapid sensorimotor learning including switching between different motor responses (i.e., nose poke versus reaching). COMPARISON WITH EXISTING METHODS: Commercial solutions are the 'go to' for rodent touchscreen behaviors, but the associated costs can be prohibitive, limiting their uptake by the wider neuroscience community. While several open-source solutions have been developed, efforts so far have focused on reducing the cost, rather than promoting ease of use and adaptability. Visiomode addresses these unmet needs providing a low-cost, extensible platform for creating touchscreen tasks. CONCLUSIONS: Developing an open-source, rapidly scalable and low-cost platform for building touchscreen-based behavioral assays should increase uptake across the science community and accelerate the investigation of cognition, decision-making and sensorimotor behaviors both in health and disease.


Assuntos
Aprendizagem , Roedores , Camundongos , Animais , Aprendizagem/fisiologia , Software , Cognição , Computadores
14.
Prog Mol Biol Transl Sci ; 195: 27-45, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36707154

RESUMO

Neurological crosstalk between the endocannabinoid and estrogen systems has been a growing topic of discussion over the last decade. Although the main estrogenic ligand, estradiol (E2), influences endocannabinoid signaling in both male and female animals, the latter experiences significant and rhythmic fluctuations in E2 as well as other sex hormones. This is referred to as the menstrual cycle in women and the estrus cycle in rodents such as mice and rats. Consisting of 4 distinct hormone-driven phases, the rodent estrus cycle modulates both endocannabinoid and exogenous cannabinoid signaling resulting in unique behavioral outcomes based on the cycle phase. For example, cannabinoid receptor agonist-induced antinociception is greatest during proestrus and estrus, when circulating and brain levels of E2 are high, as compared to metestrus and diestrus when E2 concentrations are low. Pain processing occurs throughout the cerebral cortex and amygdala of the forebrain; periaqueductal grey of the midbrain; and medulla and spine of the hindbrain. As a result, past molecular investigations on these endocannabinoid-estrogen system interactions have focused on these specific brain regions. Here, we will bridge regional molecular trends with neurophysiological evidence of how plasma membrane estrogen receptor (ER) activation by E2 leads to postsynaptic endocannabinoid synthesis, retrograde signaling, and alterations in inhibitory neurotransmission. These signaling pathways depend on ER heterodimers, current knowledge of which will also be detailed in this review. Overall, the aim of this review article is to systematically summarize how the cannabinoid receptors and endocannabinoids change in expression and function in specific brain regions throughout the estrus cycle.


Assuntos
Endocanabinoides , Estradiol , Ratos , Feminino , Masculino , Camundongos , Animais , Estradiol/farmacologia , Estradiol/metabolismo , Endocanabinoides/metabolismo , Roedores , Estro/fisiologia , Estrogênios/metabolismo , Encéfalo/metabolismo
15.
Nat Commun ; 14(1): 453, 2023 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-36707519

RESUMO

Cerebrospinal fluid (CSF) is essential for the development and function of the central nervous system (CNS). However, the brain and its interstitium have largely been thought of as a single entity through which CSF circulates, and it is not known whether specific cell populations within the CNS preferentially interact with the CSF. Here, we develop a technique for CSF tracking, gold nanoparticle-enhanced X-ray microtomography, to achieve micrometer-scale resolution visualization of CSF circulation patterns during development. Using this method and subsequent histological analysis in rodents, we identify previously uncharacterized CSF pathways from the subarachnoid space (particularly the basal cisterns) that mediate CSF-parenchymal interactions involving 24 functional-anatomic cell groupings in the brain and spinal cord. CSF distribution to these areas is largely restricted to early development and is altered in posthemorrhagic hydrocephalus. Our study also presents particle size-dependent CSF circulation patterns through the CNS including interaction between neurons and small CSF tracers, but not large CSF tracers. These findings have implications for understanding the biological basis of normal brain development and the pathogenesis of a broad range of disease states, including hydrocephalus.


Assuntos
Hidrocefalia , Nanopartículas Metálicas , Animais , Ouro/metabolismo , Roedores , Microtomografia por Raio-X , Encéfalo/metabolismo , Líquido Cefalorraquidiano/metabolismo
16.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 37(1): 120-126, 2023 Jan 15.
Artigo em Chinês | MEDLINE | ID: mdl-36708125

RESUMO

Objective: To summarize the research progress on rodent models of cervical spinal cord injury (SCI). Methods: The relevant domestic and foreign literature in recent years was reviewed, the methods of establishing the rodent models of cervical SCI and the evaluation methods of behavior, imaging, neuroelectrophysiology, and histology were summarized. Results: Cervical SCI involves primary and secondary injuries. Primary cervical SCI can be simulated with contusion, contusion compression, fracture dislocation, spinal cord traction, and spinal cord transection; scondary cervical SCI can be simulated with photochemical model and excitotoxicity model. Certain evaluation methods such as behavior, imaging, neuroelectrophysiology, and histology are used to evaluation during model building and research. Conclusion: Different rodent models of cervical SCI have different advantages and application directions, and it is critical importance for the study of cervical SCI to establish effective animal models.


Assuntos
Medula Cervical , Contusões , Traumatismos da Medula Espinal , Animais , Roedores , Medula Cervical/patologia , Traumatismos da Medula Espinal/complicações , Medula Espinal , Contusões/complicações , Contusões/patologia , Modelos Animais de Doenças
17.
Rev Bras Parasitol Vet ; 32(1): e017022, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36629665

RESUMO

Gastrointestinal parasites are well-documented in small mammals from north-central Chile, but little is known about endoparasites of rodents in southern Chile. A survey was conducted between January and February 2018 to evaluate gastrointestinal parasites and risk factors of wild rodents that live in rural areas in Northern Chiloé Island, Chile. A total of 174 fecal samples from rodents of six native and one introduced species were collected and examined using the Mini-FLOTAC method. Also, 41 individuals of four native wild rodent species were examined furtherly to determinate adult parasites from gastrointestinal tracts. The overall prevalence of endoparasites was 89.65% (156). Helminth egg types included: Rodentolepis spp., Capillariidae, Trichuris sp., Syphacia sp., oxyurid-type eggs, Strongyloides sp., Spirurid-type eggs, Strongilid-type eggs, Moniliformis sp., and an unidentified nematode egg and larvae. Protozoa comprised coccidia, amoeba, and unidentified cysts. From necropsies, adult parasites involved Syphacia sp. Trichuris sp., Protospirura sp. and Physaloptera sp. In Abrothrix olivacea, individuals with low-body-mass index exhibited reduced infection probability for Spirurid-type and Strongilid-type eggs. Some parasites in this study may affect human health. In rural settings where environmental conditions are changing, more research should be undertaken to understand parasitic infections in wildlife and implications for public health and conservation.


Assuntos
Helmintos , Enteropatias Parasitárias , Parasitos , Doenças dos Roedores , Animais , Chile/epidemiologia , Fezes/parasitologia , Enteropatias Parasitárias/epidemiologia , Enteropatias Parasitárias/veterinária , Enteropatias Parasitárias/parasitologia , Doenças dos Roedores/epidemiologia , Doenças dos Roedores/parasitologia , Roedores/parasitologia
18.
Sci Adv ; 9(2): eadd4623, 2023 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-36630502

RESUMO

Lipid nanoparticle (LNP)-based mRNA delivery holds promise for the treatment of inherited retinal degenerations. Currently, LNP-mediated mRNA delivery is restricted to the retinal pigment epithelium (RPE) and Müller glia. LNPs must overcome ocular barriers to transfect neuronal cells critical for visual phototransduction, the photoreceptors (PRs). We used a combinatorial M13 bacteriophage-based heptameric peptide phage display library for the mining of peptide ligands that target PRs. We identified the most promising peptide candidates resulting from in vivo biopanning. Dye-conjugated peptides showed rapid localization to the PRs. LNPs decorated with the top-performing peptide ligands delivered mRNA to the PRs, RPE, and Müller glia in mice. This distribution translated to the nonhuman primate eye, wherein robust protein expression was observed in the PRs, Müller glia, and RPE. Overall, we have developed peptide-conjugated LNPs that can enable mRNA delivery to the neural retina, expanding the utility of LNP-mRNA therapies for inherited blindness.


Assuntos
Nanopartículas , Roedores , Camundongos , Animais , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ligantes , Retina/metabolismo , Peptídeos/metabolismo , Primatas
19.
Stem Cell Res Ther ; 14(1): 3, 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36600321

RESUMO

BACKGROUND: Alzheimer's disease (AD) is a common progressive neurodegenerative disease characterized by memory impairments, and there is no effective therapy. Neural stem/progenitor cell (NSPC) has emerged as potential novel therapy for AD, and we aim to explore whether neural stem/progenitor cell therapy was effective for rodent models of AD. METHODS: We searched PubMed, Embase, Cochrane Library and Web of Science up to December 6, 2022. The outcomes included cognitive function, pathological features and BDNF. The GetData Graph Digitizer software (version 2.26) was applied to extract numerical values, and RevMan 5.3 and Stata 16 were used to analyze data. The SYRCLE risk of bias tool was used to assess study quality. RESULTS: We evaluated 22 mice studies and 8 rat studies. Compared to control groups, cognitive function of NSPC groups of both mice studies (SMD = - 1.96, 95% CI - 2.47 to - 1.45, I2 = 75%, P < 0.00001) and rat studies (SMD = - 1.35, 95% CI - 2.11 to - 0.59, I2 = 77%, P = 0.0005) was apparently improved. In mice studies, NSPC group has lower Aß deposition (SMD = - 0.96, 95% CI - 1.40 to - 0.52, P < 0.0001) and p-tau level (SMD = - 4.94, 95% CI - 7.29 to - 2.95, P < 0.0001), higher synaptic density (SMD = 2.02, 95% CI 0.50-3.55, P = 0.009) and BDNF (SMD = 1.69, 95% CI 0.61-2.77, P = 0.002). Combined with nanoformulation (SMD = - 1.29, 95% CI - 2.26 to - 0.32, I2 = 65%, P = 0.009) and genetically modified (SMD = - 1.29, 95% CI - 1.92 to - 0.66, I2 = 60%, P < 0.0001) could improve the effect of NSPC. In addition, both xenogeneic and allogeneic transplant of NSPC could reverse the cognitive impairment of AD animal models. CONCLUSIONS: Our results suggested that NSPC therapy could improve the cognitive function and slow down the progression of AD. Due to the limitations of models, more animal trials and clinical trials are needed.


Assuntos
Doença de Alzheimer , Doenças Neurodegenerativas , Camundongos , Ratos , Animais , Doença de Alzheimer/terapia , Roedores , Fator Neurotrófico Derivado do Encéfalo/genética , Células-Tronco
20.
Methods Mol Biol ; 2616: 3-12, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36715922

RESUMO

Rodent ischemic stroke models are essential research tools for studying this highly prevalent disease and represent a critical element in the translational pipeline for development of new therapies. The majority of ischemic stroke models have been developed to study the acute phase of the disease and neuroprotective strategies, but a subset of models is better suited for studying stroke recovery. Each model therefore has characteristics that lend itself to certain types of investigations and outcome measures, and it is important to consider both explicit and implicit details when designing experiments that utilize each model. The following chapter briefly summarizes the known aspects of the main rodent stroke models with emphasis on their clinical relevance and suitability for studying recovery and neural repair following stroke.


Assuntos
AVC Isquêmico , Acidente Vascular Cerebral , Animais , Roedores , Acidente Vascular Cerebral/terapia , Recuperação de Função Fisiológica , Modelos Animais de Doenças
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