Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 61.668
Filtrar
1.
Cornea ; 42(1): 27-31, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-35942529

RESUMO

PURPOSE: The aim of this study was to evaluate the efficacy and safety of the Yogurt technique for Descemet membrane endothelial keratoplasty (DMEK) graft preparation. METHODS: This study included patients who underwent DMEK combined or not with phacoemulsification surgery using donor tissues prepared by a surgeon with the DMEK Tzamalis disposable punch at 2 referral hospitals between October 2019 and June 2021. Primary outcomes were duration of graft preparation, surgeon grading of graft quality, and endothelial cell density measured at 1 and 6 months after surgery. Secondary outcomes were best-corrected visual acuity (BCVA) measured at 1 and 6 months postoperatively and DMEK intraoperative and postoperative complications. RESULTS: Forty-nine patients were included in this study. The mean DMEK graft preparation time was 7.2 ± 1.4 minutes. Donor grafts were rated good in 67.3% of cases (33 of 49), sufficient in 24.5% (12 of 49), and poor in 8.2% (4 of 49). Donor mean endothelial cell density was 2580 ± 155 preoperatively, which were reduced to 2269 ± 191 ( P < 0.000) and 1697 ± 142 ( P < 0.0001) at 1 and 6 months, respectively. No significant difference was found in graft preparation outcome ( P = 0.543). The preoperative BCVA was 0.65 ± 0.44 logMAR, which improved to 0.31 ± 0.43 logMAR ( P < 0.0001) and 0.12 ± 0.14 logMAR ( P < 0.0001) at 1 and 6 months after surgery, respectively. CONCLUSIONS: This study shows that the DMEK Tzamalis punch can be used as an effective and safe method for DMEK graft preparation with a shallow learning curve that allows it to be successfully performed by relatively inexperienced surgeons.


Assuntos
Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior , Distrofia Endotelial de Fuchs , Humanos , Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior/métodos , Lâmina Limitante Posterior/cirurgia , Endotélio Corneano/transplante , Iogurte , Acuidade Visual , Contagem de Células , Estudos Retrospectivos , Distrofia Endotelial de Fuchs/cirurgia
2.
Exp Neurol ; 359: 114259, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36309123

RESUMO

Neural stem cells (NSCs) implanted into sites of spinal cord injury (SCI) extend very large numbers of new axons over very long distances caudal to the lesion site, and support partial functional recovery. Newly extending graft axons distribute throughout host gray and white matter caudal to the injury. We hypothesized that provision of trophic gradients caudal to the injury would provide neurotrophic guidance to newly extending graft-derived axons to specific intermediate and ventral host gray matter regions, thereby potentially further improving neural relay formation. Immunodeficient rats underwent C5 lateral hemisection lesions, following by implants of human NSC grafts two weeks later. After an additional two weeks, animals received injections of AAV2-BDNF expressing vectors three spinal segments (9 mm) caudal to the lesion in host ventral and intermediate gray matter. After 2 months additional survival, we found a striking, 5.5-fold increase in the density of human axons innervating host ventral gray matter (P < 0.05) and 2.7-fold increase in intermediate gray matter (P < 0.01). Moreover, stem cell-derived axons formed a substantially greater number of putative synaptic connections with host motor neurons (P < 0.01). Thus, trophic guidance is an effective means of enhancing and guiding neural stem cell axon growth after SCI and will be used in future experiments to determine whether neural relay formation and functional outcomes can be improved.


Assuntos
Células-Tronco Neurais , Traumatismos da Medula Espinal , Ratos , Humanos , Animais , Fator Neurotrófico Derivado do Encéfalo , Axônios/patologia , Células-Tronco Neurais/transplante , Neurônios Motores/patologia , Interneurônios/patologia , Medula Espinal/patologia , Regeneração Nervosa/fisiologia
3.
Clin Plast Surg ; 50(1): 171-179, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36396256

RESUMO

Refined buttock augmentation with fat grafting in Chinese women during the past decade is introduced. The ideal buttock contouring outcome and figure silhouette come from the proper individualized plan and meticulous maneuvers through fat grafting to the buttocks combined with liposuction procedures on the surrounding areas of the buttocks as well as on the other body parts. The fat grafts are collected, filtered, and condensed by gravitation in a sterilized canister during liposuction. It is recommended that fat grafts were only transplanted into the subcutaneous layers and with no injection into the musculatures of the buttocks. High patients' satisfaction was obtained with no major complications and fewer minor complications.


Assuntos
Tecido Adiposo , Lipectomia , Humanos , Feminino , Nádegas/cirurgia , Tecido Adiposo/transplante , Lipectomia/métodos , Transplante Autólogo , Satisfação do Paciente
4.
Clin Plast Surg ; 50(1): 19-31, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36396258

RESUMO

Autologous fat grafting and facial liposuction are widely used to reshape facial contours. But up to now, there is no surgical design idea for Asian women. The article aimed to provide a facial esthetic design to guide the combined operation of facial liposuction and fat grafting for Asians, that is, the "three-line and nine-point" design method. In retrospective reviews, the surgical results of clinical practice were satisfactory, and a more attractive facial appearance was obtained. Our technique can easily be learnt and can be mastered by the most well-qualified plastic surgeons for satisfactory outcomes.


Assuntos
Lipectomia , Feminino , Humanos , Estudos Retrospectivos , Tecido Adiposo/transplante , Face/cirurgia , Asiáticos
5.
Facial Plast Surg Clin North Am ; 31(1): 119-129, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36396282

RESUMO

The subdorsal cantilever graft (SDCG) is a costal cartilage graft that is positioned below the nasal dorsum to control the position of the nasal bones and middle nasal vault. SDCG type A is used to raise the middle nasal vault and caudal nasal bones to correct the saddle nose deformity. SDCG type B can be used to raise the entire dorsum of the nose (radix, bony vault, and middle vault) in the ethnic augmentation rhinoplasty patient. This article will discuss the indications and technique of the SDCG in dorsal preservation rhinoplasty.


Assuntos
Cartilagem Costal , Deformidades Adquiridas Nasais , Rinoplastia , Humanos , Resultado do Tratamento , Rinoplastia/métodos , Deformidades Adquiridas Nasais/cirurgia , Nariz/cirurgia , Cartilagem Costal/transplante
6.
J Biomech Eng ; 145(1)2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-35864775

RESUMO

The Ross procedure using the inclusion technique with anticommissural plication (ACP) is associated with excellent valve hemodynamics and favorable leaflet kinematics. The objective was to evaluate individual pulmonary cusp's biomechanics and fluttering by including coronary flow in the Ross procedure using an ex vivo three-dimensional-printed heart simulator. Ten porcine and five human pulmonary autografts were harvested from a meat abattoir and heart transplant patients. Five porcine autografts without reinforcement served as controls. The other autografts were prepared using the inclusion technique with and without ACP (ACP and NACP). Hemodynamic and high-speed videography data were measured using the ex vivo heart simulator. Although porcine autografts showed similar leaflet rapid opening and closing mean velocities, human ACP compared to NACP autografts demonstrated lower leaflet rapid opening mean velocity in the right (p = 0.02) and left coronary cusps (p = 0.003). The porcine and human autograft leaflet rapid opening and closing mean velocities were similar in all three cusps. Porcine autografts showed similar leaflet flutter frequencies in the left (p = 0.3) and noncoronary cusps (p = 0.4), but porcine NACP autografts versus controls demonstrated higher leaflet flutter frequency in the right coronary cusp (p = 0.05). The human NACP versus ACP autografts showed higher flutter frequency in the noncoronary cusp (p = 0.02). The leaflet flutter amplitudes were similar in all three cusps in both porcine and human autografts. The ACP compared to NACP autografts in the Ross procedure was associated with more favorable leaflet kinematics. These results may translate to the improved long-term durability of the pulmonary autografts.


Assuntos
Próteses Valvulares Cardíacas , Valva Pulmonar , Animais , Valva Aórtica/cirurgia , Autoenxertos , Fenômenos Biomecânicos , Hemodinâmica , Humanos , Valva Pulmonar/transplante , Suínos , Transplante Autólogo
7.
Theriogenology ; 195: 40-45, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36283225

RESUMO

Ovarian cryopreservation and transplantation is a fertility preservation option, but a majority of follicles were lost due to grafting process. Accelerated activation of primordial follicle and follicle apoptosis are responsible for follicle depletion following grafting. Since apoptosis and autophagy share common upstream signals, and cell switches between apoptosis and autophagy, the aim of this study was to investigate whether autophagy took place in ovaries following transplantation. Transmission electron microscopy and immunofluorescence were used to detect autophagic changes in grafted mice ovaries. Results shown that autophagosomes increased rapidly at 2 days following ovarian transplantation in granulosa cells of growing follicles, and stromal cells. The study indicated that autophagy is actively involved during ovarian grafting. Manipulation of autophagy might provide another way to improve ovarian reserved during grafting.


Assuntos
Preservação da Fertilidade , Ovário , Camundongos , Feminino , Animais , Ovário/transplante , Folículo Ovariano/fisiologia , Criopreservação/veterinária , Criopreservação/métodos , Preservação da Fertilidade/veterinária , Autofagia
8.
Science ; 378(6622): 853-858, 2022 11 25.
Artigo em Inglês | MEDLINE | ID: mdl-36423279

RESUMO

Immune cells are being engineered to recognize and respond to disease states, acting as a "living drug" when transferred into patients. Therapies based on engineered immune cells are now a clinical reality, with multiple engineered T cell therapies approved for treatment of hematologic malignancies. Ongoing preclinical and clinical studies are testing diverse strategies to modify the fate and function of immune cells for applications in cancer, infectious disease, and beyond. Here, we discuss current progress in treating human disease with immune cell therapeutics, emerging strategies for immune cell engineering, and challenges facing the field, with a particular emphasis on the treatment of cancer, where the most effort has been applied to date.


Assuntos
Transferência Adotiva , Engenharia Celular , Neoplasias Hematológicas , Linfócitos T , Humanos , Neoplasias Hematológicas/terapia , Linfócitos T/imunologia , Linfócitos T/transplante , Transferência Adotiva/métodos
9.
Science ; 378(6622): 848-852, 2022 11 25.
Artigo em Inglês | MEDLINE | ID: mdl-36423287

RESUMO

A new era of biological engineering is emerging in which living cells are used as building blocks to address therapeutic challenges. These efforts are distinct from traditional molecular engineering-their focus is not on optimizing individual genes and proteins as therapeutics, but rather on using molecular components as modules to reprogram how cells make decisions and communicate to achieve higher-order physiological functions in vivo. This cell-centric approach is enabled by a growing tool kit of components that can synthetically control core cell-level functional outputs, such as where in the body a cell should go, what other cells it should interact with, and what messages it should transmit or receive. The power of cell engineering has been clinically validated by the development of immune cells designed to kill cancer. This same tool kit for rewiring cell connectivity is beginning to be used to engineer cell therapies for a host of other diseases and to program the self-organization of tissues and organs. By forcing the conceptual distillation of complex biological functions into a finite set of instructions that operate at the cell level, these efforts also shed light on the fundamental hierarchical logic that links molecular components to higher-order physiological function.


Assuntos
Engenharia Celular , Terapia Baseada em Transplante de Células e Tecidos , Imunoterapia Adotiva , Neoplasias , Linfócitos T , Humanos , Neoplasias/terapia , Terapia Baseada em Transplante de Células e Tecidos/métodos , Imunoterapia Adotiva/métodos , Linfócitos T/imunologia , Linfócitos T/transplante
11.
Ann Plast Surg ; 89(6): 709-715, 2022 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-36416708

RESUMO

ABSTRACT: Reconstruction of the thumb is among the most complex challenges faced by hand surgeons. Meaningful functional recovery of the thumb is dependent mainly on thumb opposition and palmar abduction. Free functional muscle transfer provides neurotized, robust soft tissue coverage that can achieve dynamic reconstruction of thenar musculature in a single stage.We present a case of a free neurotized segmental gracilis muscle transfer for thenar reconstruction and a systematic review of thenar reconstruction with free functional muscle transfer in the literature. A teenage male patient with a gunshot-induced thenar defect was reconstructed using a free neurotized gracilis muscle measuring 5 × 11 cm. The obturator nerve was coapted to the recurrent motor branch of the median nerve. The patient reached M4 strength at 10 months with functional use of his thumb. A systematic review of options for functional thenar reconstruction revealed 14 studies describing functional thenar reconstruction in 44 patients. Successful functional flaps described included gracilis muscle, pronator quadratus muscle, pectoralis minor muscle, abductor hallucis muscle, extensor digitorum brevis muscle, and serratus anterior muscle. Microsurgical transfer of a functional muscle is an excellent option for single-stage thenar reconstruction of both form and function. There are a number of available donor sites with equivalent size and bulk to that of native thenar musculature.


Assuntos
Mãos , Polegar , Adolescente , Humanos , Masculino , Polegar/cirurgia , Retalhos Cirúrgicos , Músculo Esquelético/transplante , Músculos Peitorais/transplante
12.
Ann Plast Surg ; 89(6): e69-e80, 2022 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-36416709

RESUMO

OBJECTIVE: Autologous costal cartilage is used extensively in reconstructive surgery because of its stability, durability, and biocompatibility. The current preoperative evaluation of costal cartilage often only consists of a physical examination. Several studies have highlighted the benefits of preoperative imaging as a tool to ensure optimal graft harvest. This systematic review aims to synthesize the current evidence and establish the efficacy of the various imaging modalities for the assessment of costal cartilage. REVIEW METHODS: The data sources were explored using a search strategy based on the terms ("costal cartilage" OR "ribs" AND "imaging*") combined with Boolean operators. The primary outcome measures were the ability to measure the dimensions of costal cartilages and to detect the presence of calcifications. RESULTS: A total of 28 publications were included in the final review, with 12 case series, 7 case control studies, and 9 cohort studies. Twenty-two studies used computed tomography (CT); 4 studies used x-ray and 2 studies used ultrasonography, whereas no studies used magnetic resonance imaging. Meta-analysis of the data from these studies was not deemed possible. CONCLUSIONS: Our findings suggest that CT is the modality with the strongest evidence base that provides the greatest degree of information. The major benefits of CT are its ability to provide 3-dimensional image reconstruction for surgical planning, ability to detect synchondroses, and assess cartilage quality. Where radiation exposure is less preferable, x-ray and ultrasound (US) may play an important role. X-ray appears to be particularly useful when the main concern is the presence of calcification. The limited studies available indicate that US can provide useful and accurate information on cartilage quality and morphology. Further studies are warranted in exploring the use of US in preoperative planning, particularly in the pediatric population.


Assuntos
Cartilagem Costal , Criança , Humanos , Cartilagem Costal/diagnóstico por imagem , Cartilagem Costal/transplante , Costelas , Tomografia Computadorizada por Raios X/métodos , Imageamento Tridimensional , Cartilagem/diagnóstico por imagem
13.
J Am Coll Cardiol ; 80(19): 1833-1843, 2022 11 08.
Artigo em Inglês | MEDLINE | ID: mdl-36328694

RESUMO

Approximately 95% of patients of any age undergoing contemporary, coronary bypass surgery will receive at least 1 saphenous vein graft (SVG). It is recognized that SVG will develop progressive and accelerated atherosclerosis, resulting in a stenosis, and in occlusion that occurs in 50% by 10 years postoperatively. For arterial conduits, there is little evidence of progressive failure as for SVG. Could avoidance of SVG (total arterial revascularization [TAR]) lead to a different late (>5 year) survival? A literature review of 23 studies (N = 100,314 matched patients) at a mean 8.8 years postoperative found reduced all-cause mortality for TAR (HR: 0.77; 95% CI: 0.71-0.84; P < 0.001). An expanded analysis with a new unpublished data set (N = 63,288 matched patients) was combined with the literature review (N = 127,565). It found reduced all-cause mortality for TAR (HR: 0.78; 95% CI: 0.72-0.85; P < 0.001). Additional Bayesian analysis found a very high probability of a TAR-associated reduction all-cause mortality.


Assuntos
Ponte de Artéria Coronária , Veia Safena , Humanos , Veia Safena/transplante , Teorema de Bayes , Resultado do Tratamento , Ponte de Artéria Coronária/métodos , Artérias
14.
Arthroscopy ; 38(11): 3090-3091, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36344064

RESUMO

While studies have shown significant clinical improvement after medial meniscus allograft transplantation (MMAT) with good long-term graft survivorship, progression to osteoarthritis still occurs, even in the presence of intact grafts. Several factors can potentially explain the lack of chondroprotection despite graft survivorship, including meniscal degeneration, tearing, and remodeling after the initial procedure. A major factor contributing to progression of osteoarthritis is meniscal extrusion, which is seen in up to 60% of patients and seems to be more of an issue in medial meniscus transplantation compared to lateral and is present even immediately postoperatively. Grafts without extrusion provide protective effects similar to the native meniscus, while greater than 3 mm of extrusion leads to nearly complete loss of the protective effects. A reconstruction of the meniscotibial ligament, in addition to standard MMAT, may significantly decrease meniscal extrusion. Optimization of graft size, quality, and meniscal root positioning is best to prevent extrusion and restore native biomechanics.


Assuntos
Meniscos Tibiais , Osteoartrite , Humanos , Meniscos Tibiais/transplante , Articulação do Joelho/cirurgia , Ligamentos Articulares , Aloenxertos , Imageamento por Ressonância Magnética
15.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 47(10): 1392-1397, 2022 Oct 28.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-36411690

RESUMO

OBJECTIVES: Rhinoplasty is one of the most common cosmetic surgeries in China. Septal extension grafts (SEG) have been widely used in rhinoplasty, but there are few reports on SEG derived from ear cartilage. This study aims to explore the effectiveness and stability of auricular cartilage nasal SEG transplantation in Chinese rhinoplasty. METHODS: A retrospective analysis of 35 rhinoplasty patients admitted from September 2019 to March 2022 has been conducted. Among them, 29 patients underwent rhinoplasty for the first time and 6 patients underwent rhinoplasty with the age of 18-32 (average 22.4) years old. The postoperative follow-up was 3-28 (average 18.5) months. The improvement of the nose shape was observed. The changes of the nose tip angle, nasolabial angle, and nasofrontal angle were compared between before and after the operation, and the complications were recorded. RESULTS: All patients who underwent rhinoplasty with a septal extension grafts constructed from the concha cavity and concha cartilage showed significant improvement in nasal contour. The preoperative nasal tip angle, nasolabial angle, and nasofrontal angle were significantly improved compared with 3 months after operation (all P<0.001), and there was no significant difference between 3 months and 14 months after operation (all P>0.05). The appearance of nasal cavity was satisfactory in 32 patients after operation. Columella deviation occurred in 2 patients and 1 patient complained of downward rotation of the nasal tip, which was satisfied after readjustment of the graft. CONCLUSIONS: The simplified SEG derived from auricular cartilage can provide stable support for the nasal tip, the nasal shape is natural after operation, and minimal trauma of unilateral auricle cartilage transplantation remains.


Assuntos
Procedimentos Cirúrgicos Reconstrutivos , Rinoplastia , Humanos , Adulto Jovem , Adulto , Cartilagem da Orelha/transplante , Estudos Retrospectivos , Septo Nasal/transplante
16.
Transplantation ; 106(12): 2271-2274, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36436097

RESUMO

Uterus transplantation (UTx) is currently the only available treatment for absolute uterine factor infertility. The International Society of Uterus Transplantation (ISUTx) was formally founded in 2017 and joined the Transplantation Society as a formal section in 2021. The Third International Congress of the ISUTx was held in Tübingen, Germany, in October 2021, as a hybrid meeting, attended virtually by about 450 delegates and in person by 35 delegates. This report summarizes the Tübingen meeting and complementary topics of relevance presented at the Second ISUTx state-of-the-art webinar meeting, held in Prague, in October 2020. Main topics covered included surgical considerations, including dissection of veins in living donors and the pros and cons of minimally invasive surgery; managing immune risks; UTx during the COVID-19 pandemic; lessons learnt in the areas of imaging and cytomegalovirus infection; long-term psychological outcomes; opportunities to increase organ availability; and new horizons in UTx, including potential reuse of transplants and the utilization of robotic approaches. Implementation of an International UTx Registry was discussed and considered crucial to assure quality, safety, and further progress in UTx. Attempts made thus far have been promising.


Assuntos
COVID-19 , Infertilidade Feminina , Feminino , Humanos , Infertilidade Feminina/cirurgia , Pandemias , Útero/transplante , Doadores Vivos
17.
F1000Res ; 11: 1105, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36339981

RESUMO

Introduction and importance: This report details the clinical features and management in a case of Descemet stripping automated endothelial keratoplasty (DSAEK) which had primary graft failure (PGF) due to an inverted yet attached lenticule. Presentation of case: A 66-year-old gentleman had poor visual recovery in the right eye after undergoing cataract surgery 12 years prior to presentation. The visual acuity was counting fingers and examination revealed endothelial decompensation. The patient underwent a DSAEK and postoperatively had a well attached lenticule. However, the cornea was edematous three weeks after the surgery and optical coherence tomography (OCT) revealed a reversed lenticule. The patient underwent a repeat DSAEK and had an uneventful postoperative course. The visual acuity was 20/40 after 7 months with a clear cornea and a well attached graft. Discussion: PGF is a rare complication following DSAEK which occurs due to poor endothelial function of the donor graft. Insertion of a reversed lenticule may get overlooked as a cause of PGF unless the graft edge profile is examined on an OCT scan. The graft in the current case was well attached despite its inverted position suggesting that graft adherence is perhaps not a function of the corneal endothelial pumps in isolation and may be driven by factors such as the intraocular pressure. Conclusion: A reversed DSAEK lenticule may have normal adherence to the host stroma and must be considered in cases with PGF. OCT of the graft edge is required for diagnosis before performing a repeat keratoplasty.


Assuntos
Doenças da Córnea , Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior , Masculino , Humanos , Idoso , Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior/efeitos adversos , Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior/métodos , Ceratoplastia Penetrante/efeitos adversos , Doenças da Córnea/etiologia , Doenças da Córnea/cirurgia , Endotélio Corneano/transplante , Prostaglandinas F
18.
J Neural Eng ; 19(6)2022 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-36317259

RESUMO

Objective.Acellular nerve allograft (ANA) is an effective surgical approach used to bridge the sciatic nerve gap. The molecular regulators of post-surgical recovery are not well-known. Here, we explored the effect of transgenic Schwann cells (SCs) overexpressing POU domain class 6, transcription factor 1 (POU6F1) on sciatic nerve regeneration within ANAs. We explored the functions of POU6F1 in nerve regeneration by using a cell model of H2O2-induced SCs injury and transplanting SCs overexpressing POU6F1 into ANA to repair sciatic nerve gaps.Approach.Using RNA-seq, Protein-Protein Interaction network analysis, gene ontology enrichment, and Kyoto Encyclopedia of Genes and Genomes pathway analysis, we identified a highly and differentially expressed transcription factor, POU6F1, following ANA treatment of sciatic nerve gap. Expressing a high degree of connectivity, POU6F1 was predicted to play a role in peripheral nervous system myelination.Main results.To test the role of POU6F1 in nerve regeneration after ANA, we infected SCs with adeno-associated virus-POU6F1, demonstrating that POU6F1 overexpression promotes proliferation, anti-apoptosis, and migration of SCsin vitro. We also found that POU6F1 significantly upregulated JNK1/2 and c-Jun phosphorylation and that selective JNK1/2 inhibition attenuated the effects of POU6F1 on proliferation, survival, migration, and JNK1/2 and c-Jun phosphorylation. The direct interaction of POU6F1 and activated JNK1/2 was subsequently confirmed by co-immunoprecipitation. In rat sciatic nerve injury model with a 10 mm gap, we confirmed the pattern of POU6F1 upregulation and co-localization with transplanted SCs. ANAs loaded with POU6F1-overexpressing SCs demonstrated the enhanced survival of transplanted SCs, axonal regeneration, myelination, and functional motor recovery compared to the ANA group loaded by SCs-only in line within vitrofindings.Significance.This study identifies POU6F1 as a novel regulator of post-injury sciatic nerve repair, acting through JNK/c-Jun signaling in SCs to optimize therapeutic outcomes in the ANA surgical approach.


Assuntos
Traumatismos dos Nervos Periféricos , Neuropatia Ciática , Ratos , Animais , Peróxido de Hidrogênio/metabolismo , Nervo Isquiático/metabolismo , Regeneração Nervosa/genética , Células de Schwann/fisiologia , Neuropatia Ciática/genética , Neuropatia Ciática/cirurgia , Neuropatia Ciática/metabolismo , Traumatismos dos Nervos Periféricos/genética , Traumatismos dos Nervos Periféricos/terapia , Aloenxertos/transplante , Fatores de Transcrição/metabolismo
19.
JBJS Case Connect ; 12(2)2022 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-36323500

RESUMO

CASES: We present 2 cases (19- and 40-year-old women) of lumbosacral spinal deformity with sacroiliac joint destruction. Reconstruction surgery using a fibular strut autograft was performed in both patients, and spinal reconstruction and fusion were successfully maintained without bone resorption for at least 7 years. CONCLUSION: This is the first report of reconstructive surgery using a fibular strut bone graft for lumbosacral deformity with destruction of the sacroiliac joint in patients with neurofibromatosis type 1 (NF-1). These findings suggest that reconstruction with a fibular strut autograft is useful for lumbosacral spinal deformity that includes destruction of the sacroiliac joints in NF-1.


Assuntos
Neurofibromatose 1 , Fusão Vertebral , Humanos , Feminino , Adulto Jovem , Adulto , Neurofibromatose 1/complicações , Neurofibromatose 1/cirurgia , Autoenxertos , Fíbula/transplante , Transplante Ósseo
20.
Rinsho Ketsueki ; 63(10): 1440-1445, 2022.
Artigo em Japonês | MEDLINE | ID: mdl-36351653

RESUMO

This study focused on amnion-derived mesenchymal stem cells (MSCs), which have immune- and inflammation-regulating properties, 1) a large number of stem cells, 2) high proliferative potential, and 3) are non-invasive to harvest. Based on the general research reported in many immune- and inflammation-related disease models, research on their commercial and therapeutic application was conducted. We have successfully manufactured a clinical trial product of amnion MSCs for the first time worldwide (clinical trial product name: AM01) and conducted physician-led clinical trials for acute graft versus host disease and Crohn's disease. Furthermore, CTEX Corporation, the first certified venture from Hyogo College of Medicine, was launched to further accelerate the clinical trial progression to obtain the manufacturing and marketing approval for amnion MSC AM01 to be used as a regenerative medical product at early stage.


Assuntos
Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais , Humanos , Âmnio/transplante , Terapia Baseada em Transplante de Células e Tecidos , Inflamação
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...