Use of Inert Gases for the Preservation of Nuclear Blood Cells
Braz. arch. biol. technol
; 62: e19180204, 2019. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1011539
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract The subject of the study was the stability of human white blood cell membranes subject to noble gases (xenon ad krypton, 0.6 mPa) clathrate cryoanabiosis (‒80°C). A unique portable stainless steel low pressure container with a compartment for flexible plastic container was designed to ensure that the cells are saturated with gases. The samples were warmed after 1 and 30 days in a water bath (+38°C) for 35-50 sec, while the container was being tilted (2-3 times per second), until the temperature of the biological object reached +3±1°C. It was demonstrated that after 30 days of clathrate anabiosis (-80°C) over 95% (of the original number) of leukocytes remain viable, and cell membranes of 54.5±3.4% of them is resistant to trypan blue; granulocyte survival rate is 73.5±2.7%, original lipid peroxidation rate and antioxidant activity are retained. Biological object cryopreservation in noble gases environment is a promising trend in biology and medicine.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Xenônio
/
Criopreservação
/
Leucócitos
Idioma:
Inglês
Revista:
Braz. arch. biol. technol
Assunto da revista:
Biologia
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Federação Russa
Instituição/País de afiliação:
Russian Academy of Sciences/RU