Identification of High-affinity Novel Targeted Peptides for the Treatment of Human Ovarian Cancer
Prensa méd. argent
; 105(11): 816-826, dic2019. fig, tab, graf
Artigo
em Inglês
| LILACS, BINACIS
| ID: biblio-1049970
Biblioteca responsável:
AR392.1
ABSTRACT
Background:
To investigate the potential of the phage display-identified tumor cellbinding peptide as a biomarker of epithelial ovarian cancer using phage display technology.Method:
The Ph.D.-7 Phage Display Peptide Library was used to identify the specific conjugated phages with SKOV3 epithelial ovarian cancer cells, while Chinese hamster ovary cells formed the basis. After employing the rapid differential screening method invitro, the enzyme-linked immunosorbent assay (ELISA), DNA sequencing, immunohistochemistry, immunofluorescence, and the competitive inhibition test of synthetic peptides were used to determine the affinity and specificity of the phages with SKOV3 cells.Results:
Using bio panning, we screened the phages, showing a 3590-fold increase after the third round. A total of 61 titers of the phage were randomly selected for ELISA and 10 kinds of the phages with an optical density >0.5 were used for DNA sequencing. Clones of the phage TRRNIPN were derived from DNA sequencing based on ELISA, exhibiting both the brown granular phenomenon and green fluorescence. The specific targeted peptide TRRNIPN was incorporated in tumor cells through the competitive inhibition test.Conclusion:
The results of our study indicate that the phage display identified polypeptide TRRNIPN may be an effective biomarker for the early diagnosis and targeted therapy of ovarian cancer
Coleções:
Bases de dados internacionais
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
Problema de saúde:
Meta 3.4: Reduzir as mortes prematuras devido doenças não transmissíveis
Base de dados:
BINACIS
/
LILACS
Assunto principal:
Bacteriófagos
/
DNA
/
Ensaio de Imunoadsorção Enzimática
/
Biomarcadores Tumorais
/
Programas de Rastreamento
/
Biblioteca de Peptídeos
/
Diagnóstico Precoce
/
Relatório de Pesquisa
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Prensa méd. argent
Assunto da revista:
Medicina
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
Wenzhou Medical University/CN