Improvement of the alkali stability of Penicillium cyclopium lipase by error-prone PCR
Electron. j. biotechnol
; 39: 91-97, may. 2019. ilus, graf, tab
Artigo
em Inglês
| LILACS
| ID: biblio-1052260
Biblioteca responsável:
CL1.1
ABSTRACT
BACKGROUND:
Lipases are extensively exploited in lots of industrial fields; cold-adapted lipases with alkali-resistance are especially desired in detergent industry. Penicillium cyclopium lipase I (PCL) might be suitable for applications of detergent industry due to its high catalytic efficiency at low temperature and relatively good alkali stability. In this study, to better meet the requirements, the alkali stability of PCL was further improved via directed evolution with error-prone PCR.RESULTS:
The mutant PCL (N157F) with an improved alkali stability was selected based on a high-throughput activity assay. After incubating at pH 11.0 for 120 min, N157F retained 70% of its initial activity, which was 23% higher than that of wild type PCL. Combined with the three-dimensional structure analysis, N157F exhibited an improved alkali stability under the high pH condition due to the interactions of hydrophilicity and ß-strand propensity.Conclusions:
This work provided the theoretical foundation and preliminary data for improving alkali stability of PCL to meet the industrial requirements, which is also beneficial to improving alkali-tolerance ability of other industrial enzymes via molecular modification.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Penicillium
/
Estabilidade Enzimática
/
Indústria de Detergentes
/
Lipase
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
Shandong Lonct Enzymes Co., Ltd/CN
/
Tianjin University of Science and Technology/CN