Merozoite surface protein-1 genetic diversityin Plasmodium malariae and Plasmodium brasilianum from Brazil
BMC Infectious Diseases
; 15(529): 1-11, Nov, 2015. tab, graf
Artigo
em Inglês
| Sec. Est. Saúde SP, SESSP-SUCENPROD, Sec. Est. Saúde SP
| ID: biblio-1060400
Biblioteca responsável:
BR93.2
Localização: BR93.2
ABSTRACT
The merozoite surface protein 1 (MSP1) gene encodes the major surface antigen of invasive forms of the Plasmodium erythrocytic stages and is considered a candidate vaccine antigen against malaria. Due to its polymorphisms, MSP1 is also useful for strain discrimination and consists of a good genetic marker. Sequence diversity in MSP1 has been analyzed in field isolates of three human parasites P. falciparum, P. vivax, and P. ovale. However, the extent of variation in another human parasite, P. malariae, remains unknown. This parasite shows widespread, uneven distribution in tropical and subtropical regions throughout South America, Asia, and Africa. Interestingly, it is genetically indistinguishable from P. brasilianum, a parasite known to infect New World monkeys in Central and South America. Methods:
Specific fragments (1 to 5) covering 60 % of the MSP1 gene (mainly the putatively polymorphic regions), were amplified by PCR in isolates of P. malariae and P. brasilianum from different geographic origin and hosts. Sequencing of the PCR-amplified products or cloned PCR fragments was performed and the sequences were used to construct a phylogenetic tree by the maximum likelihood method. Data were computed to give insights into the evolutionary and phylogenetic relationships of these parasites...
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Brasil
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Malária
/
Doenças Negligenciadas
Base de dados:
Sec. Est. Saúde SP
/
SESSP-SUCENPROD
Assunto principal:
Plasmodium malariae
/
Malária
Limite:
Humanos
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
BMC Infectious Diseases
Ano de publicação:
2015
Tipo de documento:
Artigo
Instituição/País de afiliação:
Universidade de São Paulo/BR
/
Wildlife Conservation Society/BR