Immune response to native NadA from Neisseria meningitidis and its expression in clinical isolates in Brazil
Int. j. med. microbiol
; 52(2): 121-125, Feb.2003.
Article
em En
| SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO
| ID: biblio-1063571
Biblioteca responsável:
BR78.1
Localização: BR78.1
ABSTRACT
A mAb against the NadA protein from Neisseria meningitidis strain 3006 (serosubtype B 2b P1.2 P5.2,8) demonstrated strong bactericidal activity against Brazilian epidemic serogroup B strain N44/89 (B 4,7 P1.19,15 P5.5,7) and a serogroup C strain, IMC 2135 (C 2a P1.5,2), but not against another serogroup C strain, N1002/90 (C 2b P1.3 P5.8). The immunogenicity of native NadA in an outer-membrane vesicle (OMV) preparation was also tested. Serum from mice immunized with OMV from serogroup B strain N44/89, which contains the NadA protein, showed bactericidal activity against serogroup B and C strains possessing NadA. In dot-blot analysis of 100 serogroup B and 100 serogroup C isolates from Brazilian patients, the mAb to NadA recognized about 60% of the samples from both serogroups. The molecular mass of the NadA protein from strain N44/89 determined by mass spectrometry was 37 971 Da and the peptide sequences were identical to those of NadA from N. meningitidis strain MC58.
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Coleções:
06-national
/
BR
Base de dados:
SES-SP
/
SESSP-IBACERVO
/
SESSP-IBPROD
Assunto principal:
Vacinas Meningocócicas
/
Neisseria meningitidis Sorogrupo B
/
Neisseria meningitidis Sorogrupo C
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Int. j. med. microbiol
Ano de publicação:
2003
Tipo de documento:
Article