Otoprotection Mechanisms Against Oxidative Stress Caused by Cisplatin
Int. arch. otorhinolaryngol. (Impr.)
; 24(1): 47-52, Jan.-Mar. 2020. graf
Artigo
em Inglês
| LILACS
| ID: biblio-1090559
Biblioteca responsável:
BR66.1
ABSTRACT
Abstract Introduction Cisplatin damages the auditory system and is related to the generation of free radicals. Glutathione peroxidase is an endogenous free radicals remover. Objective To investigate the mechanisms involved in otoprotection by N-acetylcys- teine through the expression of glutathione peroxidase in outer hair cells from rats treated with cisplatin. Methods Male Wistar rats were intraperitoneally injected with cisplatin (8 mg/Kg) and/or received oral administration by gavage of N-acetylcysteine (300 mg/Kg) for 3 consecutive days. On the 4th day, the animals were euthanized and beheaded. The tympanic bullae were removed and prepared for scanning electron microscopy and Results Among the groups exposed to ototoxic doses of cisplatin, there was an increase in glutathione peroxidase immunostaining in two groups, the one exposed to cisplatin alone, and the group exposed to both cisplatin and N-acetylcysteine. Conclusion The expression of glutathione peroxidase in the outer hair cells of rats exposed to cisplatin showed the synthesis of this enzyme under cellular toxicity conditions.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
Agenda de Saúde Sustentável para as Américas
Problema de saúde:
Objetivo 9: Redução de doenças não transmissíveis
Base de dados:
LILACS
Assunto principal:
Acetilcisteína
/
Sequestradores de Radicais Livres
/
Cisplatino
/
Estresse Oxidativo
/
Antineoplásicos
Limite:
Animais
Idioma:
Inglês
Revista:
Int. arch. otorhinolaryngol. (Impr.)
Assunto da revista:
Otorrinolaringologia
Ano de publicação:
2020
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal de Santa Maria/BR
/
Universidade de São Paulo/BR