Plasma extracellular microRNAs are related to AIDS/cerebral toxoplasmosis co-infection
Parasite immunol.
; 42(4): 12696, Apr. 2020. ilus, tab
Article
em En
| SES-SP, SESSP-IIERPROD, SES-SP
| ID: biblio-1094826
Biblioteca responsável:
BR31.1
Localização: BR31.1; 2020_P-011
ABSTRACT
This study investigated the potential of five miRNA candidates for cerebral toxoplasmosis/HIV co-infection (CT/HIV) biomarkers. miR-155-5p, miR-146a-5p, miR-21-5p, miR-125b-5p and miR-29c-3p were tested in 79 plasma divided into groups 32 CT/HIV patients; 27 individuals with asymptomatic toxoplasmosis (AT); and 20 individuals seronegative for toxoplasmosis (NC). From each was collected peripheral blood/EDTA for laboratory diagnosis. Blood cells for DNA extractions (molecular diagnosis), plasma for RNA extractions (gene expression) and ELISA (serological diagnosis). miRNA expression was performed by qPCR, and values were expressed in Relative Quantification (RQ). Among the five miRNAs, miR-21-5p and miR-146a-5p were up-expressed in CT/HIV group when compared with AT and NC groups. RQ means for miR-21-5p and miR-146a-5p in CT/HIV group were 3.829 and 2.500, while in AT group, were 1.815 and 1.661, respectively. Differences between 3 groups were statistically significant (Kruskal-Wallis ANOVA test), as well as CT/HIV and AT groups (Mann-Whitney test). Plasma of CT/HIV and AT groups expressed similar levels of miR-29c-3p, miR-155-5p and miR-125b-5p. As NC group was different of CT/HIV and AT groups, differences between three groups were statistically significant (Kruskal-Wallis ANOVA test). No difference was shown between CT/HIV and AT groups (Mann-Whitney test). These results suggest the host miRNAs modulation by Toxoplasma gondii
Palavras-chave
Texto completo:
1
Coleções:
06-national
/
BR
Base de dados:
SES-SP
/
SESSP-IIERPROD
Assunto principal:
Infecções por HIV
/
Toxoplasmose Cerebral
/
Infecções Oportunistas Relacionadas com a AIDS
/
MicroRNAs
Limite:
Humans
Idioma:
En
Revista:
Parasite immunol.
Ano de publicação:
2020
Tipo de documento:
Article