Phylogenomics and antimicrobial resistance of the leprosy bacillus Mycobacterium leprae
s.l; s.n; 2018. 11 p. mapa, tab, graf.
Não convencional
em Inglês
| Sec. Est. Saúde SP, HANSEN, Hanseníase, SESSP-ILSLPROD, Sec. Est. Saúde SP, SESSP-ILSLACERVO, Sec. Est. Saúde SP
| ID: biblio-1095218
Biblioteca responsável:
BR191.1
Localização: BR191.1; 9501/S
ABSTRACT
Leprosy is a chronic human disease caused by the yet-uncultured pathogen Mycobacterium leprae. Although readily curable with multidrug therapy (MDT), over 200,000 new cases are still reported annually. Here, we obtain M. leprae genome sequences from DNA extracted directly from patients' skin biopsies using a customized protocol. Comparative and phylogenetic analysis of 154 genomes from 25 countries provides insight into evolution and antimicrobial resistance, uncovering lineages and phylogeographic trends, with the most ancestral strains linked to the Far East. In addition to known MDT-resistance mutations, we detect other mutations associated with antibiotic resistance, and retrace a potential stepwise emergence of extensive drug resistance in the pre-MDT era. Some of the previously undescribed mutations occur in genes that are apparently subject to positive selection, and two of these (ribD, fadD9) are restricted to drug-resistant strains. Finally, nonsense mutations in the nth excision repair gene are associated with greater sequence diversity and drug resistance.
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Brasil
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Hanseníase
/
Doenças Negligenciadas
Base de dados:
HANSEN
/
Hanseníase
/
Sec. Est. Saúde SP
/
SESSP-ILSLACERVO
/
SESSP-ILSLPROD
Assunto principal:
Filogenia
/
DNA Bacteriano
/
Testes de Sensibilidade Microbiana
/
Genoma Bacteriano
/
Códon sem Sentido
/
Farmacorresistência Bacteriana
/
Anti-Infecciosos
/
Mycobacterium leprae
Tipo de estudo:
Guia de prática clínica
Limite:
Humanos
Idioma:
Inglês
Ano de publicação:
2018
Tipo de documento:
Não convencional