MicroRNA-107 is a novel tumor suppressor targeting POU3F2 in melanoma
Biol. Res
; 53: 11, 2020. graf
Artigo
em Inglês
| LILACS
| ID: biblio-1100917
Biblioteca responsável:
CL1.1
ABSTRACT
BACKGROUND:
Melanoma is one of the major types of skin cancer. The metastatic melanoma is among the most lethal forms of malignant skin tumors. We hereby aimed to characterize a novel microRNA (miR) in the metastatic melanoma model.METHODS:
First, we evaluated the expression of miR-107 in melanoma cells and tumor tissues. The comparison between primary and metastatic cancer tissues was also accessed. Next, we examined the impact of miR-107 on melanoma cell proliferation, cell cycle, colony formation, apoptotic activity, migration and matrix invasion. A downstream target of miR-107 was also predicted and validated functionally in melanoma cells.RESULTS:
Our findings showed miR-107 was significantly downregulated in melanoma. Its expression was lowest in metastatic form. Over-expression of miR-107 reduced melanoma cell proliferation, migration and invasion. POU3F2 was identified as the downstream target of miR-107. Over-expression of POU3F2 antagonized miR-107-mediated inhibitory effect on melanoma cells.CONCLUSION:
Our study has reported miR-107 as a novel tumor suppressive factor in the metastatic melanoma model. It has provided new avenue to manage melanoma and improve the survival rate in the advanced stage.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Regulação Neoplásica da Expressão Gênica
/
Genes Supressores de Tumor
/
MicroRNAs
/
Fatores do Domínio POU
/
Melanoma
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2020
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
Beijing University of Traditional Chinese Medicine/CN
/
Daqing Oilfield General Hospital/CN