Producción de celulasas por hongos: estudios cinéticos en hongos silvestres / Production of cellulase by fungi: kinetic studies on wild fungi

ABSTRACT

The kinetics of cellulase production of six fungal production of six fungal strain isolated from soils of the Lomas de Lachay (Lima), was studiend and compared with that of the hypercellulolytic mutan T. reesei QM-9414. Submerget batch conditions and lactose (1%) as inducer were used. Increment velocities of enzyme activity were comparable with those of the mutant and a significant correlation between the highest endoglucanase activity and the diameter of hydrolisis on swollen cellulose plates was found. Likewise, all fungal strains overcame the mutant in specific activity (fivefold), productivity (twofold), and B-glucosidase titer (threefold9).

RESUMEN

Seis diferentes hongos aislados de las Lomas de Lachay (Lima, Perú) fueron estudiados en su cinética de producción de celulosas y comparados con el mutante hipercelulotítico T. reesei QM-9414. Trabajando en cultivo sumergido utilizando lactosa al 1% como inductor, se detecto velocidades de incremento de la actividad enzimatica entre otros.