ADAMTS proteoglycanases downregulation with impaired oocyte quality in PCOS
Arch. endocrinol. metab. (Online)
; 65(1): 60-66, Jan.-Feb. 2021. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1152880
Biblioteca responsável:
BR1.1
ABSTRACT
ABSTRACT Objective:
A disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4) and ADAMTS-5 normal expression levels are essential for ovulation and subsequent fertilization. The objective of the present study was to assess expression pattern of these genes in cumulus cells (CCs) taken from patients with polycystic ovary syndrome (PCOS) and to investigate any possible relationship with the oocyte quality. Subjects andmethods:
ADAMTS-4 and -5 expression levels within CCs containing oocytes at the metaphase II (MII) and germinal vesicle (GV) stages, taken from 35 patients with PCOS and 35 women with normal ovarian function, were investigated using RT-qPCR. Moreover, possible correlations between ADAMTS-4, ADAMTS-5, and progesterone receptors (PRs) expression as well as oocyte quality were evaluated.Results:
ADAMTS-4 and -5 expression levels were dramatically diminished in the CCs of the PCOS patients when compared to the controls. ADAMTS-4 and -5 expression levels were correlated with each other and with the oocyte quality. Furthermore, lower expression levels of ADAMTS-4 and -5 in the PCOS patients were strongly correlated with the diminished PRs expression levels.Conclusions:
Downregulation of ADAMTS-4 and -5 in the human CCs of the PCOS patients correlated with the decline in the PRs expression, and impaired oocyte quality may cause lower oocyte recovery, maturation, and fertilization rate.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Oócitos
/
Síndrome do Ovário Policístico
/
Proteína ADAMTS4
/
Proteína ADAMTS5
Limite:
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Arch. endocrinol. metab. (Online)
Assunto da revista:
Endocrinologia
/
Metabolismo
Ano de publicação:
2021
Tipo de documento:
Artigo
País de afiliação:
Irã
Instituição/País de afiliação:
Hamadan University of Medical Sciences/IR
/
School of Medicine/IR