Electropherotypes and Subgroups of Group A Rotaviruses Circulating among Diarrhoeic Children in Kano; Nigeria
Ann. afr. med
; 7(4): 163-167, 2008.
Article
em En
| AIM
| ID: biblio-1258993
Biblioteca responsável:
CG1.1
ABSTRACT
Background:
It is estimated that about 600?000 children die annually as a result of severe dehydrating diarrhea caused by rotaviruses. The virus is a double stranded RNA (dsRNA) virus with 11 segments. Group A rotaviruses show a characteristic 4-2-3-2 pattern following electrophoresis. The VP6 subgroups; I and II exist. This work was carried out to study the prevalence of rotavirus infection among children 0-5 years with diarrhea in Kano; and to determine the circulating subgroups and electropherotypes and of the rotavirus isolates.Methods:
Two hundred and eighteen stool specimens from children 0-60 months (198 diarrheic and 20 non-diarrheic) were collected from different hospitals and health care centers in Kano and subjected to group A rotavirus enzyme linked immunosorbent assay (ELISA) to determine presence of group A rotavirus; subgroup ELISA to determine the VP6 subgroups and polyacrylamide gel electrophoresis (PAGE) to determine the electropherotypes present.Results:
The long electropherotypes (47.05) of four variations dominated over the short electropherotype (17.64). About 11.76of the isolates were of mixed infection. Dominance of subgroup II (45) over subgroup I (25); and the presence of both subgroups I and II (10) and neither subgroup I nor II (15) was observed in this study.Conclusion:
Information on the genomic diversity of the RNA electropherotypes in this region; Kano; is reported in this study
Texto completo:
1
Base de dados:
AIM
Assunto principal:
Infecções por Rotavirus
/
Criança
/
Diarreia
/
Eletroforese
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Revista:
Ann. afr. med
Ano de publicação:
2008
Tipo de documento:
Article