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A novel gene from the acidophilic bacterium Leptospirillum sp. CF-1 and its role in oxidative stress and chromate tolerance
Rivera Araya, Javier; Riveros, Matías; Ferrer, Alonso; Chávez, Renato; Levicán, Gloria.
Afiliação
  • Rivera Araya, Javier; University of Santiago of Chile (USACH). Faculty of Chemistry and Biology. Biology Department. Santiago. CL
  • Riveros, Matías; University of Santiago of Chile (USACH). Faculty of Chemistry and Biology. Biology Department. Santiago. CL
  • Ferrer, Alonso; Universidad Mayor. Facultad de Ciencias. Santiago. CL
  • Chávez, Renato; University of Santiago of Chile (USACH). Faculty of Chemistry and Biology. Biology Department. Santiago. CL
  • Levicán, Gloria; University of Santiago of Chile (USACH). Faculty of Chemistry and Biology. Biology Department. Santiago. CL
Biol. Res ; 55: 19-19, 2022. ilus, tab, graf
Artigo em Inglês | LILACS | ID: biblio-1383921
Biblioteca responsável: CL1.1
ABSTRACT
BACKGROUND: Acidophilic microorganisms like Leptospirillum sp. CF 1 thrive in environments with extremely low pH and high concentrations of dissolved heavy metals that can induce the generation of reactive oxygen species (ROS). Several hypothetical genes and proteins from Leptospirillum sp. CF 1 are known to be up regulated under oxidative stress conditions. RESULTS: In the present work, the function of hypothetical gene ABH19_09590 from Leptospirillum sp. CF 1 was studied. Heterologous expression of this gene in Escherichia coli led to an increase in the ability to grow under oxidant conditions with 5 mM K2CrO4 or 5 mM H2O2. Similarly, a significant reduction in ROS production in E. coli transformed with a plasmid carrying ABH19_09590 was observed after exposure to these oxidative stress elicitors for 30 min, compared to a strain complemented with the empty vector. A co transcriptional study using RT PCR showed that ABH19_09590 is contained in an operon, here named the "och" operon, that also contains ABH19_09585, ABH19_09595 and ABH19_09600 genes. The expression of the och operon was significantly up regulated in Leptospirillum sp. CF 1 exposed to 5 mM K2CrO4 for 15 and 30 min. Genes of this operon potentially encode a NADH:ubiquinone oxidoreductase, a CXXC motif containing protein likely involved in thiol/disulfide exchange, a hypothetical protein, and a di hydroxy acid dehydratase. A comparative genomic analysis revealed that the och operon is a characteristic genetic determinant of the Leptospirillum genus that is not present in other acidophiles. CONCLUSIONS: Altogether, these results suggest that the och operon plays a protective role against chromate and hydrogen peroxide and is an important mechanism required to face polyextremophilic conditions in acid environments.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Contexto em Saúde: Doenças Negligenciadas Problema de saúde: Doenças Negligenciadas / Zoonoses Base de dados: LILACS Assunto principal: Cromatos / Peróxido de Hidrogênio Idioma: Inglês Revista: Biol. Res Assunto da revista: Biologia Ano de publicação: 2022 Tipo de documento: Artigo País de afiliação: Chile Instituição/País de afiliação: Universidad Mayor/CL / University of Santiago of Chile (USACH)/CL

Texto completo: Disponível Coleções: Bases de dados internacionais Contexto em Saúde: Doenças Negligenciadas Problema de saúde: Doenças Negligenciadas / Zoonoses Base de dados: LILACS Assunto principal: Cromatos / Peróxido de Hidrogênio Idioma: Inglês Revista: Biol. Res Assunto da revista: Biologia Ano de publicação: 2022 Tipo de documento: Artigo País de afiliação: Chile Instituição/País de afiliação: Universidad Mayor/CL / University of Santiago of Chile (USACH)/CL
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