Sequence analysis of the cDNA encoding for SpCTx: a lethal factor from scorpionfish venom ( Scorpaena plumieri )
J. venom. anim. toxins incl. trop. dis
; 24: 1-15, 2018. ilus, tab
Artigo
em Inglês
| LILACS, VETINDEX
| ID: biblio-1484755
Biblioteca responsável:
BR68.1
Localização: BR68.1
ABSTRACT
Background:
Lethal factors are multifunctional oligomeric proteins found in the venomous apparatus of Scorpaeniformes fish. These toxins elicit not only an array of biological responses in vitro but also cardiovascular disorders and strong hemolytic, nociceptive and edematogenic activities in vivo. This work describes the cloning and molecular identification of two toxin subunits, denominated Sp-CTx- and Sp-CTx-, from scorpionfish venom ( Scorpaena plumieri ).Methods:
The primary structures were deduced after cDNA amplification by PCR with primers from conserved sequences described in Scorpaeniformes toxins. Following DNA sequencing and bioinformatic analysis, the tridimensional structures of both subunits were modeled.Results:
The translated sequences (702 amino acids, each subunit) show homology with other lethal factors, while alignment between Sp-CTx- and Sp-CTx- shows 54% identity. The subunits lack N-terminal signal sequences and display masses of approximately 80 kDa each. Both Sp-CTx subunits display a B30.2/SPRY domain at the C-terminal region with typically conserved motifs as described in these toxins. Secondary structure prediction identified six -helices 18 residues long in both and subunits, some of them amphiphilic with their N-terminal flanked by many basic residues, creating a cationic site associated with the cytolytic activity of these toxins. Antimicrobial potential sites were identified in Sp-CTx and share some features with other peptides presenting variable and broad-spectrum activity...
Texto completo:
Disponível
Base de dados:
LILACS
/
VETINDEX
Assunto principal:
Peixes Venenosos
/
DNA Complementar
/
Venenos de Peixe
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
J. venom. anim. toxins incl. trop. dis
Ano de publicação:
2018
Tipo de documento:
Artigo
/
Documento de projeto
Instituição/País de afiliação:
Universidade Federal de Minas Gerais/BR
/
Universidade Federal do Espírito Santo/BR