Cervical Intraepithelial Neoplasia grade 2 biopsy: Do p16INK4a and Ki-67 biomarkers contribute to the decision to treat? A cross-sectional study
São Paulo med. j
; 142(1): e2022527, 2024. tab, graf
Artigo
em Inglês
|
LILACS-Express
| LILACS
| ID: biblio-1509214
Biblioteca responsável:
BR1.1
ABSTRACT
ABSTRACT BACKGROUND:
Managing cervical intraepithelial neoplasia grade 2 (CIN2) is challenging, considering the CIN2 regression rate, perinatal risks associated with excisional procedures, and insufficient well-established risk factors to predict progression.OBJECTIVES:
To determine the ability of p16INK4a and Ki-67 staining in biopsies diagnosed with CIN2 to identify patients with higher-grade lesions (CIN3 or carcinoma). DESIGN ANDSETTING:
Cross-sectional study conducted at a referral center for treating uterine cervical lesions.METHODS:
In 79 women, we analyzed the correlation of p16INK4a and Ki-67 expression in CIN2 biopsies with the presence of a higher-grade lesions, as determined via histopathology in surgical specimens from treated women or via two colposcopies and two cytological tests during follow-up for untreated women with at least a 6-month interval. The expression of these two biomarkers was verified by at least two independent pathologists and quantified using digital algorithms.RESULTS:
Thirteen (16.8%) women with CIN2 biopsy exhibited higher-grade lesions on the surgical excision specimen or during follow-up. p16INK4a expression positively and negatively predicted the presence of higher-grade lesions in 17.19% and 86.67% patients, respectively. Ki-67 expression positively and negatively predicted the presence of higher-grade lesions in 40% and 88.24% patients, respectively.CONCLUSIONS:
Negative p16INK4a and Ki67 immunohistochemical staining can assure absence of a higher-grade lesion in more than 85% of patients with CIN2 biopsies and can be used to prevent overtreatment of these patients. Positive IHC staining for p16INK4a and Ki-67 did not predict CIN3 in patients with CIN2 biopsies.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Tipo de estudo:
Estudo observacional
/
Estudo de prevalência
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Estudo prognóstico
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Fatores de risco
Idioma:
Inglês
Revista:
São Paulo med. j
Assunto da revista:
Cirurgia Geral
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Cincia
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Ginecologia
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Medicina
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Medicina Interna
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Obstetr¡cia
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Pediatria
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Sa£de Mental
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Sa£de P£blica
Ano de publicação:
2024
Tipo de documento:
Artigo
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Documento de projeto
País de afiliação:
Brasil
Instituição/País de afiliação:
Center for the Clinical and Surgical Care of Women/BR
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Instituto Nacional de Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira (IFF), Fundação Oswaldo Cruz (FIOCRUZ)/BR
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Laboratory of Diagnosis Pathology and Cytopathology/BR
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Researcher in Laboratory of Diagnosis Pathology and Cytopathology/BR