Nicotine is a potent extracellular polysaccharide inducer in Fusobacterium nucleatum biofilms
Braz. j. oral sci
; 22: e231137, Jan.-Dec. 2023. ilus
Artigo
em Inglês
| LILACS, BBO - Odontologia
| ID: biblio-1523140
Biblioteca responsável:
BR218.1
ABSTRACT
The purpose of this in vitro study was to analyze the influence of nicotine on the extracellular polysaccharides in Fusobacterium nucleatum biofilm. Methods: F. nucleatum (ATCC 10953) biofilms supplemented with different concentrations of nicotine (0, 0.5, 1, 2, 4, and 8 mg/mL) were grown in two different BHI broth conditions [no sucrose and 1% sucrose]. Extracellular polysaccharides assay, pH measurements, and a spectrophotometric assay were performed. Data were submitted for ANOVA and Tukey honestly significant difference analyses (HSD) tests (α =.05). Results: Extracellular polysaccharides synthesis was influenced by an interaction between nicotine concentrations and growth medium solution containing sucrose (P<.05). The pH values declined in the sucrose-exposed biofilm were greater than in the group exposed only to nicotine (P<.05). The biofilm exposed to sucrose and nicotine had a higher total biofilm growth (P<.05) than the nicotine-treated biofilm without sucrose. Conclusions: Regardless of sucrose exposure, biofilms exposed to different nicotine concentrations influenced the amount of extracellular polysaccharides
Assuntos
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
BBO - Odontologia
/
LILACS
Assunto principal:
Polissacarídeos Bacterianos
/
Fusobacterium nucleatum
/
Biofilmes
/
Nicotina
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. oral sci
Assunto da revista:
Odontologia
Ano de publicação:
2023
Tipo de documento:
Artigo
País de afiliação:
Brasil
/
Estados Unidos
Instituição/País de afiliação:
Indiana University/US
/
University of Campinas/BR
/
University of Detroit Mercy/US