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Ultrastructural analysis and residual DNA evaluation of rabbit vein scaffold
Acta cir. bras ; 32(9): 706-711, Sept. 2017. graf
Artigo em Inglês | LILACS-Express | ID: biblio-886236
Biblioteca responsável: BR1.1


To investigate the ultrastructural characteristics and analysis of residual DNA in scaffold models, produced with decellularized vena cava in an experimental model with rabbits.


Three groups were created for ultrastructural and residual DNA analysis: group 1 - control, consisting of samples of vena cava in natura; group 2 - SD, consisting of vein fragments submitted to 2% sodium deoxycholate decellularization by shaking (160rpm - Shaker News Brunswick Scientific®) for 1 hour at controlled temperature shaker at 37°C; group 3 - SDS, consisting of vein fragments submitted to 1% sodium dodecyl sulfate decellularization under the same previous condition, for 2 hours.


The ultrastructural matrix of the blood vessel maintained its vintegrity after either decellularization models. The results of the two quantification methods demonstrated a significant decrease in the DNA content of the decellularized vena cava samples as compared to the control samples and, differed statistically from each other, p <0.05.


The 2% DS protocol for vein decellularization, in this experimental model, was considered the best protocol because it presented less amount of residual DNA without causing substantial destruction of the extracellular matrix.





Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Idioma: Inglês Revista: Acta cir. bras Assunto da revista: CIRURGIA GERAL Ano de publicação: 2017 Tipo de documento: Artigo / Documento de projeto País de afiliação: Brasil Instituição/País de afiliação: Universidade Estadual Paulista/BR