Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice
Biol. Res
; 50: 29, 2017. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-950883
Biblioteca responsável:
CL1.1
ABSTRACT
BACKGROUND:
Obsessive-compulsive disorder (OCD) is a severe neuropsychiatric condition affecting 1-3% of the worldwide population. OCD has a strong genetic component, and the SLC1A1 gene that encodes neuronal glutamate transporter EAAT3 is a strong candidate for this disorder. To evaluate the impact of reduced EAAT3 expression in vivo, we studied male EAAT3 heterozygous and wild-type littermate mice using a battery of behavioral paradigms relevant to anxiety (open field test, elevated plus maze) and compulsivity (marble burying), as well as locomotor activity induced by amphetamine. Using high-performance liquid chromatography, we also determined tissue neurotransmitter levels in cortex, striatum and thalamus-brain areas that are relevant to OCD.RESULTS:
Compared to wild-type littermates, EAAT3 heterozygous male mice have unaltered baseline anxiety-like, compulsive-like behavior and locomotor activity. Administration of acute amphetamine (5 mg/kg intraperitoneally) increased locomotion with no differences across genotypes. Tissue levels of glutamate, GABA, dopamine and serotonin did not vary between EAAT3 heterozygous and wild-type mice.CONCLUSIONS:
Our results indicate that reduced EAAT3 expression does not impact neurotransmitter content in the corticostriatal circuit nor alter anxiety or compulsive-like behaviors.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Ácido Glutâmico
/
Transportador 3 de Aminoácido Excitatório
/
Transtorno Obsessivo-Compulsivo
Limite:
Animais
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2017
Tipo de documento:
Artigo
País de afiliação:
Chile
/
Estados Unidos
Instituição/País de afiliação:
National Institute of Mental Health/US
/
Universidad Andres Bello/CL
/
Universidad de Valparaíso/CL