Effects of concentrated growth factors on the angiogenic properties of dental pulp cells and endothelial cells: an in vitro study
Braz. oral res. (Online)
; 32: e48, 2018. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-952159
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract The aim was to investigate the angiogenic effects of concentrated growth factors on human dental pulp cells and human umbilical vein endothelial cells. Cells were treated with concentrated growth factor extracts. The CCK-8 assay and cell cycle assay were conducted to evaluate cell growth. Cell migration was evaluated by the Transwell migration assay. Angiogenesis-associated mRNA and protein expression levels were determined using quantitative real-time PCR and Western blotting, respectively. A tube formation assay was conducted to evaluate the angiogenic capacity in vitro. The data showed that compared with the control, concentrated growth factor extracts significantly promoted dental pulp cell proliferation and differentiation and endothelial cell proliferation and migration in a dose-dependent manner (p < 0.05). Concentrated growth factor extracts also promoted the tube-like structure formation of endothelial cells in vitro. The RT-PCR and Western blot results showed that concentrated growth factor extracts upregulated the expression of angiogenesis-related genes - chemokine receptor-4, platelet-derived growth factor, and vascular endothelial growth factor - in dental pulp cells. In conclusion, concentrated growth factors showed proangiogenic effects on dental pulp cells and endothelial cells and have good application potential for dental pulp revascularization.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Neovascularização Fisiológica
/
Peptídeos e Proteínas de Sinalização Intercelular
/
Polpa Dentária
/
Células Endoteliais da Veia Umbilical Humana
Tipo de estudo:
Estudo de avaliação
Limite:
Adulto
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Braz. oral res. (Online)
Assunto da revista:
Odontologia
Ano de publicação:
2018
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
Chongqing Medical University/CN