The role of atenolol in the modulation of the expression of genes encoding pro- (caspase-1) and anti- (Bcl2L1) apoptotic proteins in endothelial cells exposed to intestinal ischemia and reperfusion in rats
Acta cir. bras
; 33(12): 1061-1066, Dec. 2018. tab
Artigo
em Inglês
| LILACS
| ID: biblio-973491
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract Purpose:
To investigate the role of atenolol in the gene expression of caspase 1 (Casp1) and Bcl2L1 on vascular endothelium of rat intestine after ischemia and reperfusion (IR).Methods:
Eighteen adult male Wistar rats were randomly divided into 3 groups (n=6) SG (Sham group) no clamping of the superior mesenteric artery; IRG IR plus saline group IRG+At IR plus Atenolol group. Rats from IRG and IRG+At were subjected to 60 min of intestinal ischemia and 120 min of reperfusion. Atenolol (2mg/kg) or saline were injected in the femoral vein 5 min before ischemia, 5 min and 55 min after reperfusion. Thereafter, intestinal segments were appropriately removed and processed for Endothelial Cell Biology Rat RT2 Profiler PCR Array.Results:
the anti-apoptotic Bcl2L1 gene expression was significantly down-regulated (-1.10) in the IRG and significantly up-regulated in the IRG+At (+14.15). Meanwhile, despite Casp1 gene expression was upregulated in both groups, it was significantly higher in the IRG (+35.06) than the IRG+At (+6.68).Conclusions:
Atenolol presents antiapoptotic effects on rat intestine subjected to IR partly by the up-regulation of the anti-apoptotic Bcl2L1 gene expression. Moreover, atenolol can mitigate the pro-apoptotic and pro-inflammatory effects of Casp1 gene on rat intestine after IR.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
Problema de saúde:
Doença Cardiovascular
/
Doenças do Sistema Digestório
/
Tromboembolismo Venoso
Base de dados:
LILACS
Assunto principal:
Atenolol
/
Traumatismo por Reperfusão
/
Expressão Gênica
/
Substâncias Protetoras
/
Caspase 1
/
Proteína bcl-X
/
Intestino Delgado
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2018
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
UNIFESP/BR
/
Universidade Anhembi Morumbi/BR
/
Universidade Federal de São Paulo/BR