Effect of low power laser in biomodulation of cultured osteoblastic cells of Wistar rats
Acta cir. bras
; 34(2): e201900210, 2019. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-989058
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract Purpose:
To analyze aspects of the biomodulating effect of light in biological tissues, bone cells from surgical explants of the femur of rats were irradiated with low intensity laser.Methods:
Bone cells were cultured and irradiated with LASER light (GaAlAs). Growth, cell viability, mineralized matrix formation, total protein dosage, immunostimulatory properties, cytochemical analysis, gene expression of bone proteins were examined using live cell imaging and cell counting by colorimetric assay. The gene expression of alkaline phosphatase (ALP), type 1 collagen, osteocalcin and osteopontin through the real-time polymerase chain reaction.Results:
At 8 days, the viability of the irradiated culture was 82.3% and 72.4% in non-irradiated cells. At 18 days, the cellular viability (with laser) was 77.42% and 47.62% without laser. At 8 days, the total protein concentration was 21.622 mg / mol in the irradiated group and 16, 604 mg / mol in the non-irradiated group and at 18 days the concentration was 37.25 mg / mol in the irradiated group and 24, 95 mg / mol in the non-irradiated group.Conclusion:
The laser interfered in the histochemical reaction, cell viability, matrix mineralization, and maintained the cellular expression of proteins
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Osteoblastos
/
Diferenciação Celular
/
Sobrevivência Celular
/
Terapia com Luz de Baixa Intensidade
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Brasil
/
Portugal
Instituição/País de afiliação:
UNIFESP/BR
/
Universidade Federal de São Paulo/BR
/
Universidade de Aveiro/PT
/
Universidade do Porto/PT