Dendropsophin 1, a novel antimicrobial peptide from the skin secretion of the endemic Colombian frog Dendropsophus columbianus
Nat Prod Res, v. 32, n. 12, p. 1383-1389, 2018
Article
em En
| SES-SP, SESSP-IBPROD, SES-SP
| ID: bud-2459
Biblioteca responsável:
BR78.1
ABSTRACT
In efforts to find new antimicrobial peptides (AMPs), we studied the skin secretion of the endemic Colombian frog Dendropsophus columbianus belonging to a genus that has not been investigated previously. From HPLC-fractionated secretion, we identified one peptide with slightly antibacterial activity. Its peptide sequence showed no sequence similarity to current annotated peptides. We named this novel peptide dendropsophin 1 (Dc1). Afterward, two analogues were designed (Dc1.1 and Dc1.2) to improve the cationic and amphipathic features. Then, their antiproliferative and cytotoxic properties were evaluated against several pathogens including bacteria, fungi, protozoa and also mammalian cells. Dc1 and its two analogues exhibited moderate antibacterial activities and no hemolytic and cytotoxic effects on mammalian cells. Analogue Dc1.2 showed slightly improved antibacterial properties. Their secondary structures were characterised using CD spectroscopy and Dc1.2 displayed a higher -helix content and thermal stability compared to Dc1 and Dc1.1 in hydrophobic experimental conditions.
Texto completo:
1
Coleções:
06-national
/
BR
Base de dados:
SES-SP
/
SESSP-IBPROD
País/Região como assunto:
America do sul
/
Colombia
Idioma:
En
Revista:
Nat Prod Res
Ano de publicação:
2018
Tipo de documento:
Article