Inhibition of severe acute respiratory syndrome coronavirus 2 replication by hypertonic saline solution in lung and kidney epithelial cells
ACS Pharmacol Transl Sci, v. 5, n. 4, p. 1514–1527, set. 2021
Article
em En
| SES-SP, SESSP-IBPROD, SES-SP
| ID: bud-3992
Biblioteca responsável:
BR78.1
ABSTRACT
An unprecedented global health crisis has been caused by a new virus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We performed experiments to test if a hypertonic saline solution was capable of inhibiting virus replication. Our data show that 1.2% NaCl inhibited virus replication by 90%, achieving 100% of inhibition at 1.5% in the nonhuman primate kidney cell line Vero, and 1.1% of NaCl was sufficient to inhibit the virus replication by 88% in human epithelial lung cell line Calu-3. Furthermore, our results indicate that the inhibition is due to an intracellular mechanism and not to the dissociation of the spike SARS-CoV-2 protein and its human receptor. NaCl depolarizes the plasma membrane causing a low energy state (high ADP/ATP concentration ratio) without impairing mitochondrial function, supposedly associated with the inhibition of the SARS-CoV-2 life cycle. Membrane depolarization and intracellular energy deprivation are possible mechanisms by which the hypertonic saline solution efficiently prevents virus replication in vitro assays.
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1
Coleções:
06-national
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BR
Base de dados:
SES-SP
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SESSP-IBPROD
Idioma:
En
Revista:
ACS Pharmacol Transl Sci
Ano de publicação:
2021
Tipo de documento:
Article